Rosa26{Non-EN-CFP-Neo} mouse ESC line
A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{LCA} using the Loxed Cassette Acceptor allele Rosa26{tm1.8(Non-EN-CFP)}). This ES cell line contains CFP (Cerulean) inserted into a Rosa26{LCA} allele by recombinase mediated cassette exchange. These cells were used to identifythe optimal arrangement of regulatory elements for fluorescent protein expression from a single genomic copy.
Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler
Vanderbilt University Medical Center
stem cell derived cell line