Rosa26{EN-GFP-Neo} mouse ESC line
A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{EN.GFP} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line contains eGFP inserted into the Rosa26 gene locus. These cells were used to identify an optimal combination of regulatory elements for fluorescent protein expression from a single genomic copy and can serve as a reference cell line for fluorescent cell sorting.
Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler
Vanderbilt University Medical Center
stem cell derived cell line