Rosa26{228.TOP.CFP} mouse ESC line
A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create 7xTcf/Lef binding site using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line was generated by RMCE in the Rosa26{LCA} allele. The Rosa26 gene sequence (from -228 to +81) was replaced by a Wnt response element (7xTcf/Lef binding site) fused to a TATA-Cerulean (CFP) reporter. This ES cell line may be useful for assessing canonical Wnt responsiveness.
Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler
Vanderbilt University Medical Center
stem cell derived cell line