mouse strain
Rosa26{228.3TF.GFP-CreMgn} mouse strain
A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create ROSA26{228.3TF.GFP-Cre} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse strain contains TetO-regulated genes inserted into the Rosa 26{LCA} allele by RMCE. TetO/miniCMV promoter is placed at -228 position upstream of putative ROSA26 transcription start site and drives the expression of a polycistronic mRNA with transcription factors MafA, Pdx1, and Ngn3 together with GFP-Cre fusion protein linked by 2A peptide cleavage sequences. These mice will be able to simultaneously over-express all three transcription factors and GFP-Cre upon administration of doxycyline when the effector protein rtTA is expressed.
Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler
Rosa26{228.3TF.GFP-CreMgn}
Vanderbilt University Medical Center