gene variant mutant
133 variant of FhlA
PMID:19581479
colombos:fhlA_b2731.133_VARIANT
a FhlA variant mutant with six amino acid replacements at Q11H, L14V, Y177F, K245R, M288K, and I342F. Created through random mutagenesis using error-prone PCR over the whole gene, as well as over the fhlA region encoding the first 388 amino acids of the 692-amino-acid protein. It was evolved to increase H2 production of E. coli JW2701-1(pVSC133). This is achieved by increasing transcription of all of the genes activated by FhlA (the FHL complex).