is rich medium for
synH medium
an artificial medium with a defined chemical composition designed to mimic AFEX-pretreated corn stover hydrolysate (ACSH)
synthetic hydrolisate
colombos:MEDIUM.SynH
Water (∼700 ml)
6.25 ml of 1.6 M KPO4 buffer
pH 7.2
20 ml of 1.5 M ammonium sulfate
20 ml of 2.25 M KCl
1.25 M NaCl
20 ml of a 50X amino acid stock (except tyrosine)
20 ml of 8.75 mM tyrosine dissolved in 50 mM HCl
50 ml of 1 mM each adenine, guanine, cytosine and uracil dissolved in 10 mM KOH
10 ml of vitamin stock (1 mM each thiamine, calcium pantothenate, p-aminobenzoic acid, p- hydroxybenzoic acid, and 2,3-dihydroxybenzoic acid)
1 ml of a 1000X stock of micronutrients (ZnCl2 , MnCl2 , CuCl2 , CoCl2 , H3 BO3 , (NH4 )6 Mo7 O24 , and FeCl3 )
1 ml of 1 M magnesium chloride
1ml of 90mM CaCl2
10ml of 1M sodium formate
10mM sodium nitrate
50 mM sodium succinate
1 ml of 3 M glycerol
1 ml of 500 mM lactic acid
1 ml of 700 mM glycine betaine
700 mM choline chloride
200 mM DL-carnitine (osmolytes)
5.61 g acetamide
2.71 g sodium acetate
3.3 g sodium pyruvate
2.94 g sodium citrate
1.34 g DL-malic acid
60 g D-glucose
30 g D-xylose
5.1 g D-arabinose
1.48 g D-fructose
1.15 g D- galactose
468 mg D-mannose
After adjusting to pH 7 with 10 N NaOH, the final volume is adjusted to 1L.
PMID: 25177315
microbiological culture medium
Escherichia coli K-12