clip
iclip
iclip
MI:2193
iCLIP
PSI-MI
iCLIP allows for the stringent purification of UV cross-linked protein-RNA complexes, using immunoprecipitation followed by SDS-PAGE and membrane transfer. The radiolabelled protein-RNA complexes are then excised from the membrane, and treated with proteinase to release the RNA. This leaves one or two amino acids at the RNA cross-link site. The RNA is then reverse transcribed using barcoded primers. Because reverse transcription stops prematurely at the cross-link site, iCLIP allows RNA-protein interaction sites to be identified at high resolution.
https://en.wikipedia.org/wiki/ICLIP
individual-nucleotide resolution cross-linking and immunoprecipitation
ppm
2015-06-15T11:17:22Z