two hybrid bait and prey pooling approach
barcode fusion genetics two hybrid
BGF-2h
2016-05-05T14:33:32Z
PSI-MI
This method allows screening of a full matrix of protein pairs in a single multiplexed strain pool. A doubly engineered clone pool is prepared so that each clone bears two distinct DNA barcodes flanked by site specific recombination sequences. Positive bait-prey combinations allow activation of reporter genes and their respective barcodes undergo recombination, creating unique barcode combinations. Recombined barcode tags are then fused, extracted and sequenced for identification of interacting pairs.
ppm
bfg-2h
bfg two hybrid
MI:2215
barcode fusion genetics two hybrid