]> Lead Creator: Carrine E. Blank, University of Montana, fall 2013 through spring 2016. Co-contributors and advisors: Hong Cui (University of Arizona), Lisa Moore (University of Southern Maine), and Ramona Walls (University of Arizona). MicrO (An Ontology of Prokaryotic Phenotypic and Metabolic Characters). Version 1.5.1 released 6/14/2018. Includes terms and term synonyms extracted from > 3000 prokaryotic taxonomic descriptions, collected from a large number of taxonomic descriptions from Archaea, Cyanobacteria, Bacteroidetes, Firmicutes and Mollicutes. The ontology and the synonym lists were developed to facilitate the automated extraction of phenotypic data and character states from prokaryotic taxonomic descriptions using a natural language processing algorithm (MicroPIE). MicroPIE was developed by Hong Cui, Elvis Hsin-Hui Wu, and Jin Mao (University of Arizona) in collaboration with Carrine E. Blank (University of Montana) and Lisa R. Moore (University of Southern Maine). Descriptions and links to MicroPIE can be found at http://avatol.org/ngp/nlp/overview-2/. https://github.com/biosemantics/micropie2 The most current version of MicrO can be downloaded from https://github.com/carrineblank/MicrO. Many thanks to Chris Mungall (LBNL), Elvis Hsin-Hui Wu (University of Arizona), Gail Gasparich (Towson University), and Gordon Burleigh (University of Florida) for comments and/or assistance with ontology construction and compilation of taxonomic descriptions and term definitions. Thanks to Oliver He (University of Michigan) for technical assistance with OntoBee and OntoFox, and Gareth Owen (ChEBI project leader, head curator) and other curators at ChEBI for assistance in the incorporation of microbial-specific chemical terms and synonyms into ChEBI. Thanks also to the instructors (Melissa Haendel, Matt Yoder, Jim Baihoff) and students of the 2013 NESCent Ontologies for Evolutionary Biology workshop, and to Karen Cranston (NESCent director) and the support staff at NESCent. This work was supported by a grant from the National Science Foundation Assembling the Tree of Life Program (DBI-1208534), and by a travel grant to attend the 2013 NESCent Ontologies for Evolutionary Biology workshop. is an assay using the culture medium Relationship between an assay (x) and a microbiological culture medium (y), where the medium is needed to carry out the assay. Carrine Blank has shape Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a shape (y). Carrine Blank has position Carrine Blank Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a position quality (y). has structure Carrine Blank Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a structure (y). has morphology Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a morphology (y). Carrine Blank has angle Relationship between two connected entities that are connected and have a spatial relationship defined by an angle that exists between the two connected entities. Carrine Blank is an assay using the chemical reagent Relationship between an assay (x) and a chemical entity (y), where the chemical entity is used as a chemical reagent or substance used to carry out the assay. Carrine Blank has size Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a size (y). Carrine Blank has physical quality Carrine Blank Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a physical quality (y). has texture Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a texture (y). Carrine Blank has organismal quality Carrine Blank Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and an organismal quality (y). has physical object quality Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a physical object quality (y). Carrine Blank is an enzymatic hydrolytic extract derived from Relationship between a material entity (x) and a cellular organism or environmental material (y) where the material entity is created as a result of a chemical extraction procedure performed via the process of enzymatic hydrolysis. Carrine Blank is an acid hydrolytic extract derived from Carrine Blank Relationship between a material entity (x) and a cellular organism or environmental material (y) where the material entity is created as a result of a chemical extraction procedure performed via the process of acid hydrolysis. is the quality assayed by Relationship between a quality (x) and an assay (y). Carrine Blank is culture medium used by Relationship between a microbiological culture medium (x) and a cellular organism (y) where the culture medium is used to support the growth of cellular organism y. Carrine Blank is an extract derived from Carrine Blank Relationship between a material entity (x) and a cellular organism or environmental material (y) where the material entity is created as a result of a chemical or physical extraction procedure. Carrine Blank is an oleaginous extract derived from Carrine Blank Relationship between an oleoginous (oily) material entity (x) and a cellular organism or environmental material (y) where the material entity is created as a result of a chemical extraction procedure performed from material derived from the organism. Carrine Blank is an aqueous extract derived from Carrine Blank Relationship between a material entity (x) and a cellular organism or environmental material (y) where the material entity is created as a result of a chemical extraction procedure performed via the process of an aqueous extraction (an extraction with water). Carrine Blank is an assay for the quality Carrine Blank Relationship between an assay (x) and a quality (y). uses chemical entity Relationship between a prokaryotic metabolically differentiated cell or prokaryotic metabolic process (x) and a chemical entity (y). Carrine Blank is an assay for the enzymatic substrate Carrine Blank Relationship between an assay (x) and a chemical entity (y), where the chemical entity used is a chemical enzymatic reactant in the designated process (namely a chemical reaction catalyzed by an enzyme). is an assay for the enzymatic product Carrine Blank Relationship between an assay (x) and a chemical entity (y), where the chemical entity is a chemical enzymatic product of the designated process (namely a chemical reaction catalyzed by an enzyme). is an assay for the metabolic substrate Carrine Blank Relationship between an assay (x) and a chemical entity (y), where the chemical entity is a metabolic substrate used in the designated process (generally a metabolic process deriving from a combination of enzymatic processes). is an assay for the metabolic product Carrine Blank Relationship between an assay (x) and a chemical entity (y), where the chemical entity is a metabolic product in the designated process (generally a metabolic process deriving from a combination of enzymatic processes). is metabolic substrate assayed by Carrine Blank Relationship between a chemical entity (x) and an assay (y), where the chemical entity is a metabolic substrate (generally a metabolic process deriving from a combination of enzymatic processes) assayed by the diagnostic assay. is enzymatic substrate assayed by Relationship between a chemical entity (x) and an assay (y), where the chemical entity is a chemical enzymatic reactant (namely a chemical reaction catalyzed by an enzyme) being assayed in the diagnostic assay. Carrine Blank is metabolic product assayed by Relationship between a chemical entity (x) and an assay (y), where the chemical entity is a metabolic product of the process (a metabolic process deriving from a combination of enzymatic processes) which is being assayed by the diagnostic assay. Carrine Blank is enzymatic product assayed by Relationship between a chemical entity (x) and an assay (y), where the chemical entity is a chemical enzymatic product of the enzymatic process (a chemical reaction catalyzed by an enzyme) assayed by the diagnostic assay. Carrine Blank is the enzymatic activity assayed by Carrine Blank A relationship between a molecular function (x), namely the enzymatic activity being tested and an assay (y), namely an analytical investigation/test for the presence of an enzymatic activity. is an assay for the enzymatic activity of Carrine Blank A relationship between an assay (x), namely an analytical investigation/test for the presence of an enzymatic activity, and a molecular function (y), namely the enzymatic activity being tested. is an assay for the fermentation substrate Relationship between an assay (x) and a chemical entity (y), where the chemical entity used is a fermentation substrate in the designated process (generally a metabolic fermentation process deriving from a combination of enzymatic processes). Carrine Blank is fermentation product assayed by Relationship between a chemical entity (x) and an assay (y), where the chemical entity is a fermentation product (generally a metabolic fermentation process deriving from a combination of enzymatic processes) assayed by the diagnostic assay. Carrine Blank is fermentation substrate assayed by Relationship between a chemical entity (x) and an assay (y), where the chemical entity is a fermentation substrate (generally a metabolic fermentation process deriving from a combination of enzymatic processes) being assayed by the diagnostic assay. Carrine Blank is assayed by A relationship between a process, quality, cellular component, molecular function, or a chemical entity (x) and an assay (y). Carrine Blank is an assay for Carrine Blank A relationship between a microbiological diagnostic test (x) and a biological process, quality, cellular component, molecular function, or a chemical entity (y). is an assay for the biological process of Carrine Blank A relationship between an assay (x), namely an analytical investigation/test for the presence of a physical biological process, and a biological process (y). is the biological process assayed by Carrine Blank A relationship between a biological process (x), and an assay (y) which is an analytical investigation/test for the presence of a physical biological process. is an assay for the biosynthetic production of Carrine Blank A relationship between a n assay (x), namely an analytical investigation/test for the presence of a metabolic product, and a chemical entity (y). biosynthetic production is assayed by Carrine Blank A relationship between a chemical entity (x), which is a biosynthetic product, that is assayed by an assay (y). Differentiated from 'is metabolic production of' which has the same domain and ranges, but is where an assay is used to detect a product of metabolism (i.e. energy generation). is prokaryotic physiological quality of Carrine Blank A relationship between a prokaryotic physiological quality (x) and a prokaryotic physiologically differentiated cell (y). Carrine Blank is an assay for the cellular component Carrine Blank A relationship between an assay (x), namely an analytical investigation/test for the presence of a physical cellular component, and a cellular component (y). is the cellular component assayed by A relationship between a cellular component (x), and an assay (y) which is an analytical investigation/test for the presence of a cellular component. Carrine Blank has ingredient Relationship between a material entity (x), which is a man-made material entity such as a chemical solution or a culture medium, and a chemical entity (y), where the chemical entity forms part of a mixture of chemicals that make up the material entity. Carrine Blank Wikipedia:ingredient An ingredient is a substance that forms part of a mixture (in a general sense). For example, in cooking, recipes specify which ingredients are used to prepare a specific dish. Many commercial products contain a secret ingredient that is purported to make them better than competing products. In the pharmaceutical industry, an active ingredient is that part of a formulation that yields the effect required by the customer. National laws usually require prepared food products to display a list of ingredients, and specifically require that certain additives be listed. In most developed countries, the law requires that ingredients be listed according to their relative weight[1] in the product. If an ingredient itself consists of more than one ingredient (such as the cookie pieces which are a part of "cookies and cream" flavor ice cream), then that ingredient is listed by what percentage of the total product it occupies, with its own ingredients displayed next to it in brackets. The term constituent is often chosen when referring to the substances that constitute the tissue of living beings such as plants and people, because the word ingredient in many minds connotes a sense of human agency (that is, something that a person combines with other substances), whereas the natural products present in living beings were not added by any human agency but rather occurred naturally ("a plant doesn't have ingredients"). Thus all ingredients are constituents, but not all constituents are ingredients. is an ingredient of Relationship between a chemical entity (x) and a mixture, culture medium, or chemical solution (y), where y is artificial created by human activity. Carrine Blank has susceptibility towards the chemical Carrine Blank Relationship between a prokaryotic cell or cellular component (x) and a chemical solution or chemical entity (y). develops into Relationship between an independent continuant (x) and another independent continuant (y), where x develops (or transforms) into y through a developmental process. Carrine Blank has salinity Carrine Blank Relationship between a microbiological culture medium (x) and a salinity quality (y). has pH Carrine Blank Relationship between a microbiological culture medium (x) and a pH quality (y). has redox Carrine Blank Relationship between a microbiological culture medium (x) and a redox quality (y). Carrine Blank culture medium has quality Carrine Blank Relationship between a microbiological culture medium (x) and a quality (y). has chemical composition Relationship between a material entity (x) and a material entity (y), where the material entity is a natural substance (such as a chemical entity) that has a mixture of chemical substances in it. Carrine Blank is an assay using the material entity Relationship between an assay (x) and a material entity (y), where the material entity is needed to carry out the assay. Carrine Blank is quality of the culture medium Carrine Blank Relationship between a quality (x), such as a pH quality, redox quality, or salinity quality, and a microbiological culture medium (y) which is an independent continuant. Relationship between a material entity (x) and a cellular organism or environmental material (y) where the material entity is derived from the starting complex entity y. is chemical entity derived from Carrine Blank has cell wall Carrine Blank Relationship between a particular cellular organism (x) and an external encapuslating structure (y). is an assay using the cellular organism Relationship between an assay (x) and cellular organisms (y), where the organism(s) is needed to carry out the assay. Carrine Blank uses carbon source Carrine Blank Relationship between a prokaryotic metabolically differentiated cell or prokaryotic metabolic process (x) and a chemical entity (y), where the chemical entity is a carbon source. uses energy source Relationship between a prokaryotic metabolically differentiated cell or prokaryotic metabolic process (x) and a chemical entity or sunlight (y), where the chemical entity or sunlight is a energy source. Carrine Blank uses electron donor Relationship between a prokaryotic metabolically differentiated cell or prokaryotic metabolic process (x) and a chemical entity (y), where the chemical entity is a electron donor. Carrine Blank uses electron acceptor Carrine Blank Relationship between a prokaryotic metabolically differentiated cell or prokaryotic metabolic process (x) and a chemical entity (y), where the chemical entity is an electron acceptor. working-temp obj props Carrine Blank has symmetry Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a symmetry quality (y). Carrine Blank has spatial pattern Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a spatial position quality (y). Carrine Blank contains prokaryotic metabolic process Carrine Blank Relationship between a prokaryotic metabolically differentiated cell (x) and a prokaryotic metabolic process (y). has prokaryotic physiological quality A relationship between a prokaryotic physiologically differentiated cell (x) and a prokaryotic physiological quality (y). Carrine Blank has measurement Carrine Blank xxxx is prokaryotic metabolic process carried out in the presence of Carrine Blank A relationship between a prokaryotic metabolic process (x) and an inorganic molecular entity, an organic molecular entity or sunlight (y). is prokaryotic metabolic process occuring in Relationship between a prokaryotic metabolic process (x) and a prokaryotic metabolically differentiated cell (y). Carrine Blank has prokaryotic metabolic quality Carrine Blank Relationship between a prokaryotic metabolically differentiated cell (x) and a prokaryotic metabolic quality (y). is prokaryotic metabolic quality of Relationship between a prokaryotic metabolic quality (x) and a prokaryotic metabolically differentiated cell (y) which is an independent continuant. Carrine Blank is prokaryotic metabolic quality of a cell carrying out the process A relationship between a prokaryotic metabolic quality (x) and a prokaryotic metabolic process (y). Carrine Blank is prokaryotic metabolic process which makes a cell Carrine Blank Relationship between a prokaryotic metabolic process (x) and a prokaryotic metabolic quality (y). has colony margin symmetry Relationship between a prokaryotic colony (x) and a symmetry quality (y), pertaining specifically to the symmetry of the prokaryotic colony margin. Carrine Blank contains biological process A relationship between a prokaryotic differentiated cell (x) and a biological process (y). Carrine Blank is an assay for the fermentation product Carrine Blank Relationship between an assay (x) and a chemical entity (y), where the chemical entity is a fermentation product in the designated process (generally a metabolic fermentation process deriving from a combination of enzymatic processes). has sodium chloride percentage of Carrine Blank Describes the percentage of sodium chloride concentration in which an organism is capable of growth. has cell width has pH value Carrine Blank Describes the pH (hydrogen ion concentration) in which an organism is capable of growth. zzzz Note that there is an error with this class (sulfate can't be used as an electron donor) flagellar quality Prokaryotic cell quality where a cell has either a bacterial-type flagellum (or flagella) or a periplasmic flagellum. Carrine Blank branch perpendicular to trichome Filament branch angle, where the angle between the cylindrical cells of the branching filament and the cylindrical cells of the main trichome is perpendicular. lateral branches branches perpendicular to trichome Carrine Blank branches lateral branches perpendicular to the long trichome axis filament branch size Carrine Blank branch length Filament branch morphological quality relating to the size (length, and hence the number of cells in the branch, and thickness) of a filament branch, relative to the size of the main trichome. branch size morphologically differentiated branches Carrine Blank branching morphology Filament branch physical object quality defining the morphology (shape, size, length) of branches in relationship to the main filament. straight filament branch filament branch straight Carrine Blank branches straight The nature of branches in a microbial filament to be straight (i.e. not curved). arcuate filament branch branches later curve to the direction of the original trichome Carrine Blank branches arcuate filament branch arcuate The nature of branches in a microbial filament to be arcuate (curved). solitary branches irregularly branched branches solitary Filament branch spatial pattern where the filament exhibits branching (either false or true branching). Filaments occur singularly (solitary), and do not occur in pairs (are offset from one another). Carrine Blank filaments irregularly branched paired branches Carrine Blank branches paired Filament branch spatial pattern where the filament exhibits branching (either false or true branching). Filaments occur paired, opposite from one another. heterocyte position relative to branches Carrine Blank Differentiated filament branching relating the distribution of heterocytes in the filament and their spatial relationship to the branches. branches thinner than main filament branches thinner secondary filaments thinner secondary lateral branches generally thinner thinner branches Filament branch size where the diameter of cylindrical cells in the filament branches are thicker than the diameter of cylindrical cells in the main trichome. Carrine Blank branches usually slightly thinner heterocytes basal in branches The physical relationship of branches in a microbial filament to heterocytes, being physically adjacent to heterocytes wherein branches initiate at the heterocytes. branches initiating at the heterocytes branches initiate at heterocytes Carrine Blank lateral branches initiating at the heterocytes branches initiate usually at originally intercalary heterocytes heterocytes apical in branches branches terminated by heterocytes The physical relationship of branches in a microbial filament to heterocytes, where heterocytes are located at the end of branches. Carrine Blank heterocytes not in branches branches distant from heterocytes branches distant from heterocytes distant from the heterocytes Heterocyte position where the heterocyte is not located in the filament branch. Carrine Blank heterocytes in branches Carrine Blank Heterocyte position where the heterocyte is located in the filament branch. Heterocyte location within the filament branch has apical-basal polarity. paired false branching binary false branched Carrine Blank double false branching Paired filament branches where the filament exhibits false branching. paired true branching paired true branching Paired filament branches where the filament exhibits true branching. Carrine Blank solitary false branching Solitary filament branches where the filament exhibits false branching. single false branching Carrine Blank solitary true branching Carrine Blank Solitary filament branches where the filament exhibits true branching. differentiated apical cell Filament differentiation quality, relating to the morphology and differentiation of the apical cell in the filament. Carrine Blank sub-apical cell A trichome cell that is located subterminally in a trichome, and which has undergone differentation. Found in trichomes with apical-basal polarity. Carrine Blank short filament branch Carrine Blank branches short Filament branch length where the branches are short and contain few cells (relative to the length and number of cells in the main filament). long filament branch Carrine Blank Filament branch length where the branches are long and contain many cells (relative to the length and number of cells in the main filament). branches many-celled branches long prokaryotic nucleobase quality The molar percentage of nucleobases in the DNA of a prokaryotic chromosome and/or plasmid. Carrine Blank bacilloid cell Carrine Blank bacilloid A prokaryotic cell that is bacillus-like (some variation of cylindrical). amphilophotrichous cell 2 bipolar polytrichous flagella tufts of polar flagella Flagellated cell where the cell has multiple flagella, located in two tufts at both ends (poles) of the cell. Carrine Blank polar tufts of flagella prokaryotic acidic environment acidic Carrine Blank An acidic environment that is the environment inhabited by a prokaryotic cell. prokaryotic alkaline environment An alkaline environment that is the environment inhabited by a prokaryotic cell. Carrine Blank alkaline optical quality assay An assay to determine macroscopic or microscopic optical quality of a prokaryotic cell, or aggregation of prokaryotic cells. Carrine Blank fermentation product Prokaryotic metabolite where fermentation produces are produced as a result of fermentation. cyanobacterial filament A type of filament only found in some filamentous cyanobacterial taxa. Is comprised by a trichome and an extracellular sheath. May or may not have branches. Carrine Blank isopolar symmetric An isopolar trichome morphology quality where heterocysts are only present at the poles, or terminals, of the trichome such that the trichome morphology is symmetric. Carrine Blank isopolar metameric An isopolar trichome morphology quality where heterocysts are regularly dispersed along the length of the trichome such that the trichome morphology is symmetric. Carrine Blank differentiated isopolar trichome Carrine Blank Trichome differentiation quality of isopolar trichomes with respect to the location of heterocytes and the symmetrical arrangement of the heterocytes. meristematic zone A part of a trichome where rapid cell division occurs. Meristematic zones, which arise terminally or subterminally, are found in the trichomes of Scytonemataceae, Rivulariaceae, and Microchaetaceae. Zone involves several cells along the trichome. Carrine Blank diffluent sheath A sheath that is easily dissolving and indistinct (unlocalized). Carrine Blank casein agar https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/CaseinAgar.html FORMULA Ingredients per liter of deionized water:* Dry Milk, Instant Nonfat 50.0gm Pancreatic Digest of Casein 5.0gm Yeast Extract 2.5gm Glucose 1.0gm Agar 12.5gm Final pH 6.8 +/- 0.3 at 25 degrees C. An organic-rich, solid microbiological culture medium that contains nonfat dry milk, pancreatic digest of casein (casitone), yeast extract, glucose, and agar. Used for the differentiation of prokaryotes based on their ability to degrade casein. Carrine Blank cell filament trichomous filaments filament A group of prokaryotic cells where the cells are attached to one another end-to-end, forming long threads. Formed when cell division occurs in a single plane (at right angles to the filament axis) and when cells do not detach from one another. Carrine Blank chains filamentous mycelium-like filament-forming true branch Carrine Blank A trichome part formed when there is transient cell division in another plane other than crosswise to the length of the trichome axis. This results in the formation of a branch at right angles to the axis of the length of the trichome. Found only in the Stigonematales (Cyanobacteria). false branch A trichome part formed when there is interruption of the axis of a trichome. Are initiated when necritic cells form, breaking the trichome. Or are initiated at heterocytes. At first both ends of the interrupted trichome grow aside one another, later on the two new trichome ends grow in different directions. Occurs in the Oscillatoriales, Nostocales, and Stigonematales (Cyanobacteria). Carrine Blank filament branch A filament part formed when a new trichome begins to grow at an angle to the axis of the original length of the filament, also enclosed in sheath. Carrine Blank firm sheath A sheath that is firm and distinct (localized, not easily dissolving and not indistinct). Carrine Blank sheath having distinctive spatial pattern Carrine Blank Morphologically differentiated filament part, where the sheath has a distinctive spatial pattern. thylakoid-containing cell Carrine Blank A prokaryotic cell containing thylakoids membranes. epilithic environment Carrine Blank An environment that exists on the surface of a rock (or a rocky substrate). epilithic epipelic environment epipelic Carrine Blank A submerged environment consisting of a rocky substrate comprised of sediments, clays, or silt, colonized by a microorganisms or a microbial community. submerged stones epiphytic environment Wikipedia: Epiphyte An epiphyte is a plant that grows harmlessly upon another plant (such as a tree) and derives its moisture and nutrients from the air, rain, and sometimes from debris accumulating around it. Epiphytes differ from parasites in that epiphytes grow on other plants for physical support and do not necessarily negatively affect the host. An epiphytic organism that is not a plant is called an epibiont.[1] Epiphytes are usually found in the temperate zone (e.g., many mosses, liverworts, lichens, and algae) or in the tropics (e.g., many ferns, cacti, orchids, and bromeliads).[2] Many houseplants are epiphyte species due to their minimal water and soil requirements. Epiphytes provide a rich and diverse habitat for other organisms including animals, fungi, bacteria, and myxomycetes.[3] Carrine Blank An environment that exists on a terrestrial plant (Embryophyte). epiphytic endophytic environment An environment that exists within a plant (Embryophyte). Carrine Blank endophytic Wikipedia: Endophyte An endophyte is an endosymbiont, often a bacterium or fungus, that lives within a plant for at least part of its life cycle without causing apparent disease.[1][2][3][4] Endophytes are ubiquitous and have been found in all species of plants studied to date; however, most of these endophyte/plant relationships are not well understood.[5][6] Endophytes are also known to occur within lichens[7] and algae. Many economically important grasses (e.g., Festuca spp. and Lolium spp. ) carry fungal endophytes in genus Epichloë,[8] some of which may enhance host growth[9] and may improve the plant's ability to tolerate abiotic stresses, such as drought, and enhance resistance to insects, plant pathogens and mammalian herbivores.[10][11][12][13] lives inside a plant periphytic environment periphytic Carrine Blank periphytically An environmental system that is submerged, and attached to submerged rocks, plants, animals, or other objects. calyptra Modified apical cell, having a thickened or enlarged morphology. Has a hood-like, lid-like, or cap-like form. Little is known about the role, although presence is environmentally influenced. Not all trichomes in a natural population will exhibit a calyptra. Found in the Oscillatoriales and Nostocales. Carrine Blank end cells aways with calyptra http://huey.colorado.edu/cyanobacteria/taxa/calyptra.php Definition of Calyptra Thickened or enlarged tip of a cyanobacterial filament, a hoodlike, lidlike, or caplike structure. morphologically distinct colony A prokaryotic colony that has a distinct morphology (size, shape, texture, or structure). Carrine Blank tapered by apical and basal widening trichomes narrowed in the middle part and clearly widened at the ends Carrine Blank branches dilated terminal portions Tapered trichome quality where the trichome is wider at the apical and basal ends, and narrower toward the center of the trichome (hour-glass shaped). prokaryotic cell part quality Prokaryotic quality (morphology, symmetry, position) relating to a cell part or cellular component. Carrine Blank tapered by apical and basal narrowing attenuated trichome ends trichomes attenuated towards the ends trichomes attenuated toward both ends continually attenuated to the ends trichomes attenuated at the ends of developed trichomes Carrine Blank tapering to the ends cells narrowed at both ends trichomes narrowed to the ends attenuated toward the ends Tapered trichome quality where the trichome is narrower at the apical and basal ends, and widened toward the center of the trichome (fusiform-shaped). trichomes narrows towards the ends trichomes attenuated to the ends widened spaces between thylakoids Carrine Blank intrathylakoidal spaces Thylakoid-containing cell where there are widened spaces between the thylakoid membranes. Function is not clear and are likely influenced by environmental factors. Frequency and location are taxa-specific. susceptibility to lysis by detergent Carrine Blank Prokaryotic cell wall lysis susceptibility that defines how susceptible the cell wall is to lysis when the cell is placed in the presence of an environment with an altered chemical composition (detergent). Berkefeld N filter A gravity-driven water filter made by the British Berkefeld® company, with an intermediate pore size N (for Normal). Pore sizes average 0.43 microns in diameter. Carrine Blank 216L marine medium An organic rich, liquid marine microbiological culture medium containing acetate, tryptone, yeast extract, citrate, ammonium nitrate, with a seawater base. Used for the cultivation of Marispirillum. From: Lai, Q., Yuan, J., Gu, L. & Shao, Z. (2009). Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. Int J Syst Evol Microbiol 59, 1278–1281. Recipe: sodium acetate, 1.0 g Tryptone, 10.0 g yeast extract, 2.0 g sodium citrate, 0.5 g NH4NO3, 0.2 g seawater, 1L pH 7.5 Carrine Blank 216L 216L broth 216L marine agar From: Lai, Q., Yuan, J., Gu, L. & Shao, Z. (2009). Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. Int J Syst Evol Microbiol 59, 1278–1281. Recipe: sodium acetate, 1.0 g Tryptone, 10.0 g yeast extract, 2.0 g sodium citrate, 0.5 g NH4NO3, 0.2 g seawater, 1L pH 7.5 Agar is added to the medium to solidify. Carrine Blank 216L agar An organic rich, solid marine microbiological culture medium containing acetate, tryptone, yeast extract, citrate, ammonium nitrate, with a seawater base. Used for the cultivation of Marispirillum. ZoBell marine medium ZoBell 2216e broth ZoBell 2216e ZoBell medium MA2216 Zobell's medium Marine broth 2216 An organic-rich, mineral-salts, liquid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Used for the cultivation of heterotrophic marine microorganisms. ZoBell broth From: http://himedialabs.com/TD/M384.pdf Zobell Marine Agar 2216 Zobell Marine Agar 2216 is recommended for cultivation, isolation and enumeration of heterotrophic marine bacteria. Composition Ingredients (Gms/Litre) Peptic digest of animal tissue 5.000 Yeast extract 1.000 Ferric citrate 0.1000 Sodium chloride 19.450 Magnesium chloride 8.800 Sodium sulfate 3.240 Calcium chloride 1.800 Potassium chloride 0.550 Sodium bicarbonate 0.160 Potassium bromide 0.080 Strontium chloride 0.034 Boric acid 0.022 Sodium silicate 0.004 Sodium fluorate 0.0024 Ammonium nitrate 0.0016 Disodium phosphate 0.008 Final pH (25˚C) 7.6+/-0.2 MB Carrine Blank ZoBell's microbiological culture medium A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. growth medium growth media culture media Carrine Blank media medium defined microbiological culture medium defined mineral medium defined medium Carrine Blank A chemically defined microbiological culture medium where all chemicals used are known. Does not contain any plant, animal, or microbiological cell extracts. mineral media defined media mineral medium liquid microbiological culture medium liquid media broth A microbiological culture medium that is liquid. Carrine Blank liquid medium solid microbiological culture medium solid medium solid media Carrine Blank A microbiological culture medium that is solidified with a gelling agent, such as agar, agarose, gelatin, or pectin. agar medium undefined microbiological culture medium basal media complex medium basal medium complex media A microbiological culture medium that has components that are chemically heterogeneous, unknown, or uncharacterized mixtures or cellular extracts. Carrine Blank Balch medium 1 pH is not noted in the original publication. However, pH of the medium is reported to be 7.0 in The Prokaryotes: Vol 3: Archaea. Bacteria: Firmicutes, Actinomycetes, p. 213, copyrighted 2006. mineral medium and H2 + CO2 A mineral-salts liquid microbiological culture medium containing acetate, formate, yeast extract, trypticase, cysteine and sulfide with H2 and CO2 in the headspace gas mixture. Used for the growth of methanogenic archaea. A mineral-salts, liquid microbiological culture medium containing acetate, formate, yeast extract, trypticase, cysteine and sulfide with hydrogen and carbon dioxide in the headspace gas mixture. Used for the growth of methanogenic archaea. Carrine Blank mineral medium with H2-CO2 From: Balch WE et al, 1979. Methanogens: reevaluation of a unique biological group. Microbiol Rev 43(2):260. Ingredients are added to distilled water to give a final volume of 1L. Cysteine and Na2S are added after boiling the medium under an 80% N2-20% CO2 gas mixture, the final gas phase of tubed medium being an 80% H2-CO2 gas mixture at two atmospheres of pressure. 50 mL mineral 1 solution (6 g K2HPO4 per liter) 50 mL mineral 2 solution (per liter: KH2PO4, 6 g; (NH4)2SO4, 6 g, NaCl, 12 g; MgSO4x7H2O, 2.6 g; CaCl2x2H2O, 0.16 g) 10 mL trace minerals (pH to 7.0 with KOH; per liter: nitrilotriacetic acid, 1.5 g; MgSO4x7H2O, 3.0 g; MnSO2x2H2O, 0.5 g; NaCl, 1.0 g; FeSO4x7H2O, 0.1g; CoSO4 or CoCl2, 0.1 g; CaCl2x2H2O, 0.1 g; ZnSO4, 0.1 g; CuSO4x5H2O, 0.01 g; AlK(SO4)2, 0.01 g; H3BO3, 0.01 g; Na2MoO4x2H2O, 0.01g); dissolve nitrilotriacetic acid with KOH to pH 6.5 then proceed to add minerals) 10 mL trace vitamines (contains in milligrams per liter: biotin, 2; folic acid, 2; pyridoxine hydrochloride, 10; thiamine hydrochloride, 5; riboflavin, 5; nicotinic acid, 5; DL-calcium pantothenate, 5; vitamin B12, 0.1; p-aminobenzoic acid, 5; lipoic acid, 5) 0.002 g FeSO4x7H2O 5.0 g NaHCO3 2.5g sodium acetate 2.5 g sodium formate 2.0 g yeast extract 2.0 g trypticase (BBL) 0.5 g L-Cysteine hydrochloridexH2O 0.5 g Na2Sx9H2O minerals salts-H2/CO2 medium artificial kinneret water medium artificial kinneret water A dilute, mineral-salts liquid microbiological culture medium formulated to mimic Lake Kinneret (Sea of Galilee) water. Used to cultivate freshwater autotrophic microorganisms. Could not find in any references the pH of AKW, however the pH of Lake Kinneret varies from about 8.6 at the surface to 7.8 at depth. http://kinneret.ocean.org.il/ar_grp.aspx artificial lake kinneret water AKW AKW media Carrine Blank From Table 1 in: Sherr BF, Sherr EB, Berman T. 1983. Grazing, growth, and ammonium excretion rates of a heterotrophic microflagellate fed with four species of bacteria. Appl Environ Microbiol 45(4):1196-1201. Salt Amt (mg L-1 of distilled water) NaHCO3 197 NaCl 155 CaCl2 130 MgCl2 124 MgSO4 65 KCl 13 CaSO4 9.5 Total salts, 693.5 mg L-1. Total solids in Lake Kinneret water range from 640 to 714 mg L-1. Gram stain quality Carrine Blank Prokaryotic cell part quality, describing the nature of the cell wall. Determined from the results of the Gram stain assay. MGM medium MGM liquid medium A hypersaline, liquid microbiological culture medium that contains mineral salts, peptone, and yeast extract. Used for the cultivation of Haloarchaeobium iranensis. Carrine Blank MGM broth From:Makhdoumi-Kakhki et al 2011. Haloarchaeobium iranensis gen.nov., sp. nov., an extremeley halophilic archaeon isolated from the saline lake Aran-Bidgol, Iran. Int J. Syst. Evol. MIcrobiol. doi:10.1099/ijs.0.033167-0 and Dyall-Smith. 2009. Halohandbook. http://www.haloarchaea.com/resources/halohandbook/ Add the following to a large beaker: Salt water (30% stock), 767 Pure water, 200 Peptone (Oxoid), 5 yeast extract, 1 Stir to dissolve, adjust pH up to 7.5 with 1M Tris pH 7.5, using 5 mL per liter. Adjust to 1L with pure water. For solid medium add 15g Difco Bacto-agar. 30% Salt water stock solution (the CaCl2 is not added until just before pouring plates and some TrisHCl pH 7.5 is added): per liter: H2O, 850 mL NaCl, 240 g MgCl2x6H2O, 30g KCl, 7g 1M TrisHCL pH 7.5, 20 mL modified 9K medium From: Zhou H et al. 2008. Isolation and characterization of Ferroplasma thermophilum sp. nov., a novel extremely acidophilic, moderately thermophilic archaeon and its role in bioleaching of chalcopyrite. J Appl Microbiol 105:591. Modified 9K medium contained (per litre): 3.0 g (NH4)2SO4, 0.5 g KH2PO4, 0.1 g KCl, 0.5 g MgSO4x7H2O, 0.01 g Ca(NO3)2. Trace elements (per litre) comprised: 11.0 mg FeCl3x6H2O, 0.5 mg CuSO4x5H2O, 50 mg Na2SO4, 2.0 mg H3BO3, 2.0 mg MnSO4, 0.8 mg Na2MoO4x2H2O, 0.6 mg CoCl2x6H2O, 0.9 mg ZnSO4x7H2O and 0.1 mg Na2SeO4. The pH of the medium was adjusted to 1.0 by adding 50% (v ⁄ v) H2SO4, was autoclaved, and then filter-sterilized (0.2 um filter paper) trace elements and FeSO4x7H2O were added in the medium. Carrine Blank An acidic, liquid, mineral-salts microbiological culture medium, high in ferrous iron. Used for the cultivation of Ferroplasma thermophilum. NOM-1 medium NOM-1 liquid medium From: Heng-Lin Cui, Xin-Yi Li, Xia Gao, Xue-Wei Xu, Yu-Guang Zhou, Hong-Can Liu, Aharon Oren and Pei-Jin Zhou. 2010. Halopelagius inordinatus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. IJSEM 60:2089-2093. per liter: yeast extract, 0.2 g fish peptone, 0.2 g sodium pyruvate, 2.0 g sodium lactate, 2.0 g KCl, 5.4 g K2HPO4, 0.3 g NH4Cl, 0.5 g MgSO4x7H2O, 20.0 g NaCl, 200.0 g pH 7.0-7.2. neutral oligotrophic haloarchaeal medium A hypersaline, liquid microbiological culture medium containing mineral-salts, magnesium sulfate, pyruvate, lactate, peptone, and yeast extract. Used for the cultivation of Halopelagius inordinatus. Carrine Blank modified R2A medium A hypersaline, liquid microbiological culture medium containing mineral-salts, magnesium sulfate, pyruvate, glucose, glutamate, citrate, and peptones. Used for the cultivation of Halogranum rubrum and Halosarcina limi. CM2 medium CM2 liquid medium From: Cui H-L, et al. 2010. Halogranum rubrum gen. nov., sp. nov., a halophilic archaeon isolated from a marine solar saltern. Int J Syst Evol Microbiol 60:1366. Modified R2A medium: Contains the following ingredients per liter: Casamino acids 0.5g yeast extract, 0.5g sodium pyruvate, 0.5 g fish peptone, 0.5g glucose, 0.5g sodium glutamate, 0.5g trisodium citrate, 3.0g KCl, 2.0g K2HPO4, 0.3g CaCl2, 0.5g MgSO4x7H2O, 20.0g NaCl, 200.0 g pH 7.0-7.2 Carrine Blank MR2A From: Cui H-L et al. 2010. Halosarcina limi sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halosarcina. Int J. Syst. Evol. Microbiol. 60:2462. CM2 Recipe: Per liter: Casamino acids (Difco), 0.5 g yeast extract (Difco), 0.5 g sodium pyruvate, 0.5 g fish peptone, 0.5 g glucose, 5.0 g sodium glutamate, 0.5 g trisodium citrate, 3.0 g KCl, 2.0 g K2HPO4, 0.3 g CaCl2, 0.5 g MgSO4x7H2O, 20 g NaCl, 230.0 g pH 7.0–7.2 MR2A liquid medium defined inorganic chemical mixture Inorganic compounds or mixtures of inorganic compounds added to culture media to support growth or metabolism of a microorganism. Because the exact composition of the mixture is known, it is referred to as "defined". Carrine Blank DSMZ Medium 954 Halococcus dombrowskii medium DSM strains: 19505 Haladaptatus cibarius 18796 Halalkalicoccus jeotgali B3 14522 Halococcus dombrowskii 19504 Halorubrum cibi 18794 Haloterrigena jeotgali A29 18795 Natronococcus jeotgali DSM 18795 Medium for Halococcus dombrowskii Carrine Blank From: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium954.pdf 954. Medium for Halococcus dombrowskii. Casamino acids 5.00 g Yeast extract 5.00 g TRIS 12.10 g KCl 2.00 g MgCl2x6H2O 20.00 g CaCl2x2H2O 0.20 g NaCl 200.00 g Agar 20.00 g Distilled water 1000.00 mL Adjust pH to 7.4. Add the agar after dissolving all ingredients in the water and adjustment of pH. © 2007 DSMZ GmbH - All rights reserved A hypersaline, organic-rich, solid microbiological culture medium containing mineral-salts, tris, casamino acids, and yeast extract. Used to cultivate Halococcus dombrowskii (DSM 14522). Hayflick medium From:http://www.atcc.org/~/media/B61DC4844DBE4D51867A06D49DE22564.ashx ATCC Medium: 2820 HAYFLICK Medium Yeast Extract…………………………………..19.6 g PPLO…………………………………………...17.7 g Phenol Red……………………………………24 mg DNA sodium salt (Sigma, D1501)………...…0.24 g Horse serum (aseptically add)……………….157 ml DI Water………………………………………..843 ml Combine all ingredients except for the horse serum. Adjust pH to 7.8 +/- 0.2. Autoclave at 121C and let medium cool to 55C. Aseptically add horse serum and dispense as required. Carrine Blank An organic-rich, liquid microbiological culture medium containing beef heart infusion, peptone, and sodium chloride. Developed for the growth of Mycoplasma pneumoniae. nutrient broth From: Nutrient Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Nutrient Broth has the formula originally designed for use in the Standard Methods for the Examination of Water and Wastewater. It is not a recommended bacteriological medium in later editions of this publication. It is one of several nonselective media recommended for use in the Most Probable Number (MPN) technique of estimating the density of viable organisms in food samples1 and is useful in routine cultivation of microorganisms. Principles of the Procedure This relatively simple formulation supports the growth of nonfastidious microorganisms due to its content of peptone and beef extract. Formula Difco™ Nutrient Broth Approximate Formula* Per Liter Beef Extract.................................................................. 3.0 g Peptone....................................................................... 5.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.8 ± 0.2 Carrine Blank nutrient medium NB An organic-rich, liquid culture medium comprised of beef extract and peptone. nutrient broth nutrient broth media ZoBell phytagel From: http://himedialabs.com/TD/M384.pdf Zobell Marine Agar 2216 Zobell Marine Agar 2216 is recommended for cultivation, isolation and enumeration of heterotrophic marine bacteria. Composition Ingredients (Gms/Litre) Peptic digest of animal tissue 5.000 Yeast extract 1.000 Ferric citrate 0.1000 Sodium chloride 19.450 Magnesium chloride 8.800 Sodium sulfate 3.240 Calcium chloride 1.800 Potassium chloride 0.550 Sodium bicarbonate 0.160 Potassium bromide 0.080 Strontium chloride 0.034 Boric acid 0.022 Sodium silicate 0.004 Sodium fluorate 0.0024 Ammonium nitrate 0.0016 Disodium phosphate 0.008 Final pH (25˚C) 7.6+/-0.2 Barbeyron T, L'Haridon S, Corre E, Kloareg B & Potin P. 2001. Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. IJSEM 51:985-997.: When it was desirable to avoid attack of the substratum, strains were grown on ZoBell solidified with 0.7% (w/v) Phytagel (a gellan gum; Sigma). Carrine Blank An organic-rich, mineral-salts, liquid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Solidified using Phytagel (gellan gum) instead of agar. Used for the cultivation of heterotrophic marine microorganisms capable of degrading agar. ZoBell phytagel plates ZoBell phytagel agar medium containing ruminal fluid Carrine Blank A microbiological culture medium that contains bovine rumen fluid (or ruminal fluid), used for the culture of microorganisms that live in the rumen. Sehgal and Gibbons medium Sehgal and Gibbons, 1960, Effect of some metal ions on the growth of Halobacterium cutirubrum. Can J Microbiol 6:165. A hypersaline, organic-rich liquid medium containing casamino acids, yeast extract, and salts. Used for the growth of Halobacterium cutirubrum. Original citation: Sehgal SN, Gibbons NE (1960) Effect of some metal ions on the growth of Halobacterium cutirubrum. Can J Microbiol 6:165–169. The routine medium contained 7.5 g casamino acids (Difco), 10 g yeast extract (Difco), 3 g trisodium citrate, 2 g potassium chloride, 20 g magnesium sulphate heptahydrate, 250 g sodium chloride, aid distilled water to 1 liter. The ingredients were dissolved in 800 ml water, the pH adjusted to 7.5-7.8 with N potassium hydroxide, and the medium autoclaved 5 minutes at 120˚C. It was then filterecl to remove the precipitate, the pH mas adjusted to 7.4 with N hydrochloric acid, and the medium made up to final volume. S-G medium From: Schneegurt MA, Chapter 2, Media and conditions for the growth of halophilic and halotolerant bacteria and archaea. pg. 39, Table 2.1. In Vreeland RH (ed) Advances in Understanding the Biology of Halophilic Microorganisms. DOI 10.1007/978-94-007-5539-0_2 Medium composition in grams per liter: NaCl, 250 MgSO4x7H2O, 20 FeCl2, 0.023 Na-citrate, 3 Casamino acids, 7.5 yeast extract, 10 Carrine Blank Brucella agar with sheep blood From: Brucella Agar with 5% Sheep Blood, Hemin and Vitamin K1 (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Brucella Agar with 5% Sheep Blood, Hemin and Vitamin K1 is used for the isolation and cultivation of fastidious, obligately anaerobic microorganisms. BRU Carrine Blank An organic-rich, solid microbiological culture medium, containing peptones, yeast extract, glucose, and sodium bisulfite as a reducing agent. Supplemented with 5% sheep blood, hemin, and vitamin K. Used for the growth of Brucella. Zobell phytagel starch Starch phytagel plates Carrine Blank Zobell phytagel starch agar An organic-rich, mineral-salts, liquid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Solidified using Phytagel instead of agar. Supplemented with starch. Used for the cultivation of heterotrophic marine microorganisms capable of degrading agar. Barbeyron T, L'Haridon S, Corre E, Kloareg B & Potin P. 2001. Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. IJSEM 51:985-997. The strain was assayed for amylase activity using starch at a concentration of 0.2 % (w/v) in ZoBell agar or in ZoBell Phytagel plates. BRN medium Balch et al. 1979. Methanogens: reevaluation of a unique biological group. Microbiol Rev 43(2):260. A liquid, mineral-salts microbiological culture medium containing acetate, formate, yeast extract, trypticase, cysteine and sulfide with H2 and CO2 in the headspace gas mixture. Supplemented with additional 0.1% NH4Cl, 10% rumen fluid, 2% agar, and with clindamycin and cephalotin (cefalotin). Used for the growth of Methanobrevibacter from feces. Miller, 2001. In Bergey's Manual of Systematic Bacteriology, v. 1, 2nd ed. p.219. Carrine Blank Miller TL et al, 1982. Isolation of Methanobrevibacter smithii from human feces. Appl Environ Microbiol 43(1):227. MGM agar From:Makhdoumi-Kakhki et al 2011. Haloarchaeobium iranensis gen.nov., sp. nov., an extremeley halophilic archaeon isolated from the saline lake Aran-Bidgol, Iran. Int J. Syst. Evol. MIcrobiol. doi:10.1099/ijs.0.033167-0 and Dyall-Smith. 2009. Halohandbook. http://www.haloarchaea.com/resources/halohandbook/ Add the following to a large beaker: Salt water (30% stock), 767 Pure water, 200 Peptone (Oxoid), 5 yeast extract, 1 Stir to dissolve, adjust pH up to 7.5 with 1M Tris pH 7.5, using 5 mL per liter. Adjust to 1L with pure water. For solid medium add 15g Difco Bacto-agar. 30% Salt water stock solution (the CaCl2 is not added until just before pouring plates and some TrisHCl pH 7.5 is added): per liter: H2O, 850 mL NaCl, 240 g MgCl2x6H2O, 30g KCl, 7g 1M TrisHCL pH 7.5, 20 mL A hypersaline, organic-rich, solid microbiological culture medium that contains mineral-salts, tris, peptone, and yeast extract. Used for the cultivation of Haloarchaeobium iranensis. Carrine Blank microbiological culture medium containing blood blood agar BAP Blood agar plates A microbiological culture medium that contains blood, treated blood, lysed blood cells, blood serum, or treated blood serum. Carrine Blank citrated blood Carrine Blank From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Animal Blood, Citrated is blood that has been washed and treated with sodium citrate as the anticoagulant. It serves as a source of erythrocytes for serological procedures. Blood medium ingredient comprised of animal blood where citrate has been added to prevent coagulation. Used in the cultivation of microorganisms. equine serum-containing microbiological culture medium Carrine Blank media containing 1% serum fraction A liquid microbiological culture medium that contains horse (equine) serum. horse serum containing agar media containing 20% horse serum microbiological culture medium, containing procine serum Carrine Blank A microbiological culture medium (a blood serum agar), made with porcine (pig) blood serum. PPLO medium PPLO serum fraction PPLO media PPLO broth An organic-rich, liquid microbiological culture medium containing various peptones and sodium chloride. Developed for the growth of mycoplasmas. From: PPLO Media (Mycoplasma Media) (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use PPLO (Mycoplasma) agars and broths, when supplemented with nutritive enrichments, are used for isolating and cultivating Mycoplasma. Mycoplasma Broth Base (Frey) is used for the cultivation of avian mycoplasmas. Principles of the Procedure Meat digests, peptones, beef extract and yeast extract provide the nitrogen, vitamins, amino acids and carbon in these media. Sodium chloride maintains the osmotic balance of these formulations. Agar, the solidifying agent, is used in PPLO (Mycoplasma) Agar at a concentration slightly reduced from usual to ensure formation of the largest possible colonies because the organisms grow into the agar with only slight surface growth.13 The base media are supplemented with Mycoplasma Supplement or Mycoplasma Enrichment w/o Penicillin because Mycoplasma spp. are fastidious in their growth requirements. 14 Mycoplasma Supplement contains fresh yeast extract and horse serum. Yeast extract provides the preformed nucleic acid precursors that are required by Mycoplasma spp.14 Horse serum supplies cholesterol, a growth stimulant.14 Mycoplasma Enrichment without Penicillin is a selective enrichment containing the inhibitor thallium acetate, to which a penicillin of choice (penicillin G or a broad-spectrum semisynthetic penicillin) can be added at the time of use to make it selective against gram-positive and gram-negative bacteria. Formulae Difco™ PPLO Agar Approximate Formula* Per Liter Beef Heart, Infusion from 50 g..................................... 6.0 g Peptone..................................................................... 10.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 14.0 g Difco™ PPLO Broth Consists of the the same ingredients without the agar. pH 7.8 ± 0.2 BBL™ Mycoplasma Agar Base (PPLO Agar Base) Approximate Formula* Per Liter Beef Heart, Infusion from (solids).................................. 2.0 g Pancreatic Digest of Casein.......................................... 7.0 g Beef Extract.................................................................. 3.0 g Yeast Extract................................................................ 3.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 14.0 g pH 7.8 ± 0.2 BBL™ Mycoplasma Broth Base (PPLO Broth Base) Consists of the same ingredients without the agar. BBL™ Mycoplasma Broth Base (Frey) Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 7.5 g Papaic Digest of Soybean Meal..................................... 2.5 g Yeast Extract................................................................ 5.0 g Sodium Chloride.......................................................... 5.0 g Potassium Chloride...................................................... 0.4 g Magnesium Sulfate...................................................... 0.2 g Disodium Phosphate.................................................... 1.6 g Monopotassium Phosphate.......................................... 0.1 g pH 7.7 ± 0.2 Difco™ Mycoplasma Supplement Approximate Formula* Per 30 mL Vial Yeast Extract................................................................ 0.09 g Horse Serum.............................................................. 22.8 mL BBL™ Mycoplasma Enrichment without Penicillin Approximate Formula* Per 30 mL Vial Horse Serum.............................................................. 20.0 mL Yeast Extract (fresh autolysate)................................... 10.0 mL Thallium Acetate........................................................ 50.0 mg *Adjusted and/or supplemented as required to meet performance criteria. Carrine Blank SP-4 medium An organic-rich, liquid microbiological culture medium containing pancreatic digests of casein and gelatin, PPLO broth (without CV, i.e. without crystal violet), fetal bovine serum (fetal calf serum), CMRL 1066 medium (a mixture of amino acids, vitamins, co-factors, and organic micronutrients), yeast extract, and polymyxin B, Amphotericin B, and penicillin. Used for the growth of mycoplasmas. Carrine Blank SP-4 media From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/SP4Media.htm SP4 Broth, Plain**: Pancreatic Digest of Casein 10.0gm Pancreatic Digest of Gelatin 5.0gm PPLO Broth without CV 3.5gm Polymyxin B 50.0mg Amphotericin B 5.0mg Fetal Bovine Serum 170.0ml CMRL 1066 Medium (10X) 50.0ml Yeast Extract 35.0ml Yeastolate 10% 20.0ml Penicillin 1,000,000U Final pH at 25 degrees C.: SP4 Broth, Plain SP4 Broth with Arginine 7.0 +/- 0.2 horse blood agar Carrine Blank A solid microbiological culture medium (a blood agar), containing horse blood. horse blood agar slant culture medium A slant culture is where liquified (melted) solid medium has been added to a sterile test tube and allowed to solidify (cool) at an angle with respect to the horizontal, such that the solid medium surface is at an angle with respect to the sides of the test tube. The slant culture allows for a larger surface of the agar to be exposed to oxygen. blue green medium blue-green medium Carrine Blank BG media A mineral-salts, marine, liquid microbiological culture medium containing soil extract. Used for the growth of photoautotrophic microorganisms. http://www-cyanosite.bio.purdue.edu/media/table/bg.html From: Moss,B. 1972. The influence of environmental factors on the distribution of freshwater algae: an experimental study. J. Ecol. 60:917-932. Blue-Green (BG) Medium This medium is made up in 2 parts. Autoclave parts 1 and 2 separately at 15 psi, allow to cool them then mix aseptically. For agar plates, add 15 g non-nutrient agar per L. Part 1: Tricine 0.50 g Soil extract (SE1) 25.00 mL Extra nutrient salts 3.75 mL Filtered natural seawater to 1.0 L Adjust pH to 7.6-7.8 with 1M NaOH or HCl. Part 2: NaNO3 1.500 g K2HPO4x3H2O 0.040 g MgSO4x7H2O 0.075 g CaCl2x2H2O 0.036 g Citric acid 0.006 g Ammonium ferric citrate green 0.006 g EDTANa2 0.001 g Na2CO3 0.020 g Trace metal solution 1.00 mL Distilled water to 1.0 L Adjust pH to 7.4. Extra nutrient salts: NaNO3 3.00 g Na2HPO4 0.12 g K2HPO4 0.10 g Distilled water to 100.0 mL Trace metal solution: H3BO3 0.286 g MnCl2x4H2O 0.022 g Na2MoO4x2H2O 0.039 g CuSO4x5H2O 0.008 g Co(NO3)2x6H2O 0.005 g Distilled water to 100 mL BG medium Lindstrom medium L-medium No. 16 Lindstrom media A mineral-salts, liquid microbiological culture medium comprised mainly of sodium bicarbonate. Used to cultivate autotrophic microorganisms from lakes. L 16 Carrine Blank From: Lindstrom, K. 1983. Selenium as a growth factor for plankton algae in laboratory experiments and in some Swedish lakes. Hydrobiologia 101:35-48. Macronutrients (all values mg per L) NaHCO3 84.00 NaNO3 12.75 CaCl2x2H2O 14.70 MgSO4x7H2O 12.32 K2HPO4 1.74 Na2SiO3x9H2O 1.14 Micronutrients FeCl3x6H2O 0.108 Na2MoO4x2H2O 0.048 ZnCl2 0.027 MnCl2x4H2O 0.010 CoCl2x6H2O 0.0096 Na2SeO3x5H2O 0.000184 EDTA 0.558 pH 8.1 Aa medium A mineral-salts, liquid microbiological culture medium containing sodium acetate, potassium phosphate, sodium carbonate, ammonium sulfate, cysteine hydrochloride, sodium sulfide, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, nitrilotriacetic acid, manganese sulfate, cobalt chloride, zinc sulfate, resazurin, copper sulfate, potassium aluminum sulfate, boric acid, sodium molybdate, pyridoxine hydrochloride, thiamine hydrochloride, riboflavin, nicotinic acid, p-aminobenzoic acid (4-aminobenzoic acid), lipoic acid, biotin, folic acid, and vitamin B12 (cobalamin). Prepared under an atmosphere of dinitrogen and carbon dioxide. Used for the growth of Methanosaeta concillii. Carrine Blank From: Patel GB. 1984. Characterization and nutritional properties of Methanothrix concilii sp. nov., a mesophilic, aceticlastic methanogen. Can J Microbiol 30:1383-1396. The composition of the Aa medium used for isolation and stock culture maintenance was as follows (in milligrams per litre): CH3COONa·3H2O, 6800; K2HPO4, 2190; KH2PO4, 1500; Na2CO3, 480; (NH4)2SO4, 450; cysteine-HCl, 125; Na2S·9H2O, 125; MgSO4·7H2O, 120; CaCl2·2H2O, 60; NaCl, 54; FeSO4 ·7H2O, 21; nitrilotriacetic acid, 15; MnSO4 · 2H2O, 5; CoCl2-6H2O, 1; ZnSO4·7H2O, I; resazurin, I; CuSO4·5H2O, 0.1; AIK(SO4)2- 12H2O, 0.1; H3BO3, 0.1; Na2Mo04·2H2O, 0.1; pyridoxine-HCl, 0.1; thiamine-HCl, riboflavin, nicotinic acid, p-aminobenzoic acid, lipoic acid, each 0.05; biotin and folic acid, each 0.02; vitamin B-12, 0.005. The Aa medium ingredients, except Na2CO3 and cysteine- Na2S, were 'mixed and adjusted to pH 7.8. The medium was then reduced with cysteine- Na2S, and Na2CO3 was added, using the Hungate (1950) technique. The reduced medium was dispensed in 10-mL aliquots into 60-mL serum vials (Miller and Wolin 1974) under 80% N2: 20% CO2 gas phase. The postautoclave (121°C, 15 min) pH was 7.2 ± 0.1. If the same medium contained 10000 mg/L of CH3COONa·3H20, it was referred to as AA medium. prokaryotic quality Carrine Blank Quality that inheres in a prokaryotic cell, group of cells, prokaryotic colony, or part of a prokaryotic cell. L-proline arylamidase assay using nitroanilide L-proline 4-nitroanilide Carrine Blank L-proline arylamidase that uses the substrate L-proline 4-nitroanilide (L-proline-p-nitroanilide). Proline arylamidase activity (which could be from proline aminopeptidase as well as other dipeptidase enzymes) cleaves the substrate, releasing 4-nitroaniline which is bright yellow in color. A positive test is yellow; a negative test is colorless. Proline p-nitroanilide PRO L-proline-p-nitroanilide water filter A filter with a specified pore size that filters solid material above the specified pore size out of a liquid medium. Carrine Blank Berkefeld filter candle A gravity-driven water filter made by the British Berkefeld® company. Carrine Blank prokaryotic cell quality Physical object quality of a prokaryotic cell. Carrine Blank trichome pole Carrine Blank A trichome part, refering to the poles (terminal ends) of a prokaryotic trichome. May involve one cell or multiple cells along the trichome. mesophilic Carrine Blank Temperature optimum quality, where growth rates at moderate temperatures (20-40˚C) are higher than growth rates at other temperatures. ZoBell marine agar MA From: http://himedialabs.com/TD/M384.pdf Zobell Marine Agar 2216 Zobell Marine Agar 2216 is recommended for cultivation, isolation and enumeration of heterotrophic marine bacteria. Composition Ingredients (Gms/Litre) Peptic digest of animal tissue 5.000 Yeast extract 1.000 Ferric citrate 0.1000 Sodium chloride 19.450 Magnesium chloride 8.800 Sodium sulfate 3.240 Calcium chloride 1.800 Potassium chloride 0.550 Sodium bicarbonate 0.160 Potassium bromide 0.080 Strontium chloride 0.034 Boric acid 0.022 Sodium silicate 0.004 Sodium fluorate 0.0024 Ammonium nitrate 0.0016 Disodium phosphate 0.008 Final pH (25˚C) 7.6+/-0.2 MA2216 agar MA plates ZMA MA solid medium An organic-rich, mineral-salts, solid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Used for the cultivation of heterotrophic marine microorganisms. Carrine Blank Marine agar MA medium marine agar 2216 Marine 2216 agar Zobell marine agar Zobell 2216E agar tryptic soy broth Trypticase soy broth From: Bacto™ Tryptic Soy Broth/Trypticase™ Soy Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Tryptic (Trypticase) Soy Broth (Soybean-Casein Digest Medium) is a general purpose medium used in qualitative procedures for the cultivation of fastidious and nonfastidious microorganisms from a variety of clinical and nonclinical specimens. Principles of the Procedure Enzymatic digests of casein and soybean provide amino acids and other complex nitrogenous substances. Dextrose is an energy source. Sodium chloride maintains the osmotic equilibrium. Dibasic potassium phosphate acts as a buffer to control pH. The addition of 6.5% sodium chloride to Trypticase Soy Broth permits the differentiation of salt-tolerant enterococci, which are resistant to the high salt content, from the salt-intolerant S. bovis group and other streptococcal species. At this concentration, the sodium chloride is a selective agent that interferes with membrane permeability and osmotic and electrokinetic equilibria.4 Fildes Enrichment is a peptic digest of sheep blood that supplies the X (hemin) and V (nicotinamide adenine dinucleotide, NAD) factors necessary for the growth of H. influenzae. Dextrose is omitted from the formula for Tryptic Soy Broth without Dextrose to permit use of the medium in fermentation studies. The carbohydrate concentration used most frequently in fermentation reactions is 0.5% or 1%. Tryptic Soy Broth and Trypticase Soy Broth are provided as prepared media in a variety of bottle styles. In addition, Tryptic Soy Broth is provided as a Sterile Pack Bottle; i.e., the bottle has been terminally sterilized inside of autoclavable double-bags. All varieties of bottled TSB conform with requirements for Ready- To-Use Media as described in the USP. Formulae Bacto™ Tryptic Soy Broth (Soybean-Casein Digest Medium) Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 17.0 g Papaic Digest of Soybean............................................. 3.0 g Dextrose...................................................................... 2.5 g Sodium Chloride.......................................................... 5.0 g Dipotassium Phosphate................................................ 2.5 g pH 7.3 ± 0.2 BBL™ Trypticase™ Soy Broth (Soybean-Casein Digest Broth) Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 17.0 g Papaic Digest of Soybean............................................. 3.0 g Sodium Chloride.......................................................... 5.0 g Dipotassium Phosphate................................................ 2.5 g Dextrose...................................................................... 2.5 g pH 7.3 ± 0.2 Bacto™ Tryptic Soy Broth without Dextrose Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 17.0 g Enzymatic Digest of Soybean Meal............................... 3.0 g Sodium Chloride.......................................................... 5.0 g Dipotassium Phosphate................................................ 2.5 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.2 Soya broth media Tryptone soya broth An organic-rich, liquid culture medium comprised of pancreatic digest of casein (casitone), papaic digest of soybean (soy peptone), dextrose (D-glucose), sodium chloride, and dipotassium phosphate (potassium dibasic phosphate). Tryptic soy broth Wikipedia:Tryptic_soy_broth Tryptic soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic bacteria. It is frequently used in commercial diagnostics in conjunction with the additive Thio which promotes growth of anaerobes. Soya broth tryptic soy medium TSB Carrine Blank tryptic soy agar TSBA plates Tryptone soy agar TSA Tryptone Soya Agar TSA agar Tryptic soy broth agar Trypticase soy broth agar Trypticase soy agar TSBA Tryptic soy agar Trypticase-Soy agar An organic-rich, solid medium containing casitone (pancreatic digest of casein), Phytone peptone (papaic digest of soybean), and sodium chloride. Used for the cultivation of aerobic heterotrophic microorganisms. From: Tryptic Soy Agar/Trypticase™ Soy Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Tryptic (Trypticase) Soy Agar (TSA) is used for the isolation and cultivation of nonfastidious and fastidious microorganisms. It is not the medium of choice for anaerobes. Principles of the Procedure The combination of casein and soy peptones in TSA renders the medium highly nutritious by supplying organic nitrogen, particularly amino acids and longer-chained peptides. The sodium chloride maintains osmotic equilibrium. Agar is the solidifying agent. Haemophilus species may be differentiated by their requirements for X and V factors. Paper strips impregnated with these factors are placed on the surface of the medium after inoculation with the test organism. Following incubation, a zone of growth around the strip indicates a requirement for the factor(s). Formulae Difco™ Tryptic Soy Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 15.0 g Papaic Digest of Soybean............................................. 5.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g pH 7.3 ± 0.2 BBL™ Trypticase™ Soy Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 15.0 g Papaic Digest of Soybean............................................. 5.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. Carrine Blank prokaryotic metabolic process Carrine Blank A single-organism metabolic process that is present in a prokaryotic metabolically differentiated cell. chemical mixture, serving as microbiological medium ingredients Carrine Blank A solid or liquid mixture of chemicals which are used as ingredients in microbiological culture media used to grow and propogate prokaryotic microorganisms. sub-cylindrical A flattened prokaryotic cell where the shape is partially spherical. sub-cylindrical thermophilic Carrine Blank Temperature optimum quality, where growth rates at elevated temperatures (40-85˚C) are higher than growth rates at ambient temperatures. filament septation Carrine Blank A filament of prokaryotic cells relating to the presence or absence of septa separating the individual cells that make up the filament. cyanobacterial filament part Carrine Blank Cell part of a cyanobacterial filament. filamentous distinctively shaped filament Carrine Blank A cell filament that has a distinctive shape. spatially differentiated filaments A filament morphological quality where the filaments have a particular spatial orientation with respect to a substrate or surface. Carrine Blank scytonematoid-branched filaments loop-formation loop formation Scytonema-like false branching filaments in loop-like formations common lateral loops Scytonema-type false scytonematoid branching S-type scytonematoid false branching False-branched filaments, where branches initiate at a necridic cell. Branches often appear as pairs. Carrine Blank filaments make sometimes typical loop-like lateral formations tolypothrixoid-branched filaments False-branched filaments, where branches initiate at a heterocyte. Branches only sometimes appear as pairs. tolypotrichoid branched Tolypothrix-type false branching Tolypothrix-like false branching Carrine Blank Tolypothrix-type Tolypothrix-like false branched T-type X-branched filaments X-branching Carrine Blank Chlorogloeopsis-type branching True-branched filaments, where the cells divide irregularly in different planes and form clusters of cells. The cells remain physiologically attached. irregular clustering T-branched filaments T-type branching Carrine Blank perpendicular branching true branching lateral (usually unilateral) laterally true branched T-type of branching branching type "T" type of branching: T-type branching of T-type erect true branches develop usually unilaterally after the lengthwise cell division true branching lateral Westiella-type lateral T-branching irregularly lateral true branched lateral T-type branching lateral Hapalosiphon-type branching lateral (T-type) Stigonema-type Fischerella-type zzzz 'inheres in' some (Stigonema or Fischerella or Hapalosiphon or Westiella) Westiella is in NCBI Tax browser, but not in OntoFox. True-branched filaments, where a cell in the trichome divides longitudinally (parallel to the axis of the filament) and the trichome branch starts to grow perpendicular to one side of the main trichome. T-branching V-branched filaments pseudodichotomous branches arise by longitudinal division of the apical cells Hyphomorpha-type branching type "V" Loriella-type pseudodichotomously true branched pseudodichotomously divaricated branches Carrine Blank zzzz 'inheres in' some (Hyphomorpha or Pulvinularia or Colteronema or Loriella or Mastigocoleopsis) - no NCBI entries. pseudodichotomously branched pseudodichotomously divaricated at the ends V-branching Mastigocoleopsis-type pseudodichotomous branchings pseudodichotomously divaricated V-type V-type of branching pseudodichotomous V-type True-branched filaments, where an apical cell divides longitudinally, from which grows obliquely two equivalent branches. branching lateral (V-type) pseudodichotomously divaricated free filaments pseudodichotomous reverse Y-branched filaments Carrine Blank reverse Y-like branching Y-type True-branched filaments, where branches arise in the intercalary (medial) cells after oblique cell division at an intercalary (medial cell). reverse Y-type Y-branching zzzz 'inheres in' some (Scytonema or Brachytrichia or Mastigocladus) Brachytrichia is in NCBI Tax Browser, but not in OntoFox. reverse Y-branching biseriate filament 2 2 Filament with one trichome in the sheath, where the trichome is two cells in width. Arises as a result of cell division occuring in two planes,one perpendicular to the axis of the trichome and one parallel to the axis of the trichome. bi-seriate Carrine Blank with 2 cells aside extracellular sheath An extracellular sheath in cyanobacteria is typically a laminated (lamellated, laminar), fibrillar, extracellular structure made of polysaccharides (glycan). It plays a role in desiccation tolerance and often contains pigments (such as scytonemin) which protect from photodamage caused by uv light. It also has proteins (including water stress proteins, pectases). Often multilayered and colored. Carrine Blank sheath trichome Wikipedia: trichome Algal trichomes Certain, usually filamentous, algae have the terminal cell produced into an elongate hair-like structure called a trichome. The same term is applied to such structures in some cyanobacteria, such as Spirulina and Oscillatoria. Cyanobacteria trichomes may be unsheathed, as in Oscillatoria, or sheathed, as in Calothrix.[1] These structures play an important role in preventing soil erosion, particularly in cold desert climates.[citation needed] The filamentous sheaths form a persistent sticky network that helps maintain soil structure. Carrine Blank A prokaryotic filament part, comprised of a chain of cells that has undergone binary fission in a single plane, where the cells remain attached after cell division. Found in the Nostocales and Stigonematales (Cyanobacteria). isopolar trichome apical cells not differentiated A prokaryotic trichome where the two poles of the trichome have the cell diameter (i.e., are symmetrical and not tapered). Caused by the development of a basal heterocyte. trichomes isopolar morphologically not diversified in "main" filaments and branches simple trichomes trichomes not distinctly diversified filaments simple Carrine Blank simple filaments apical cells morphologically not different from other cells heteropolar trichome filaments polarized A prokaryotic trichome where the two poles of the trichome have different cell diameters (i.e. trichomes are tapered on end end and asymmetrical). Caused by the development of a basal heterocyte. trichomes heteropolar filaments differentiated into basal and apical parts trichomes, oriented by their bases with heterocytes to their substrate and by their apical hir-like parts to the surface Carrine Blank free apical end filaments distinctly divided into basal end with a heterocyte and the apical end differentiating into basal and apical parts apical cell Carrine Blank free apical end A trichome cell that is located terminally in a trichome, and which has undergone differentiation. Found in trichomes with apical-basal polarity. susceptibility to lysis by hypotonic solution Prokaryotic cell wall lysis susceptibility that defines how susceptible the cell wall is to lysis when the cell is placed in the presence of an environment with an altered chemical composition (hypotonic solution). Carrine Blank basal prostrate filaments Filament orientation quality where erect filaments and prostrate filaments are present, refers to the basal filaments that are prostrate to the substrate. Carrine Blank necritic cell nectrotic cells Carrine Blank trichomes disintegrating in separated cells within filaments trichomes often disintegrate soon after heterocyte formation between heterocytes A trichome cell comprised of a cell that has undergone differentiation such that it is dead (nectritic). chroococcoid cell cluster chroococcoidal chroococcoid clusters of cells old trichomes change often in chroococcoid stages chroococcoid stage known chroococcal stages chroococcoid cell clusters arise Cell clusters, where the cells are chroococcoid (spherical). Carrine Blank differentiated meristematic zone not all cells capable of dividing not all cells capable to divide distinct merstematic zones Filament differentiation quality with respect to the positioning of the meristematic zone along the length of the trichome. not all cells capable of division Carrine Blank tapered trichome trichomes variable width Carrine Blank trichomes tapered Heteropolar trichome where the width of the trichome varies such that the trichome is tapered. trichome tapering hair cell apical hairs An apical cell or cells in a heteropolar filament that is differentiated to form an elongated hair-like extension. long tapering to the hair-like ends hair-like apical ends long hairs hair-like ends terminal hairs hyaline hairs in the apical part narrowed in elongated, cellular hair elongated hyaline cells hair formation hyaline cells cellular hair hairs Carrine Blank trichomes elongated in cellular hair-like ends apical hair-like ends hair-cells vacuolized apical cell vacuolized cells at the ends apical cells vacuolated vacuolized apical cells terminal cells vacuolized end cells sometimes vacuolated Carrine Blank Apical cell that is vacuolized (has gas vacuoles). morphologically distinctive apical cell Cyanobacterial filament part, where the apical cell is morphologically distinctive. Carrine Blank conical-rounded apical cell end cells conical-rounded rounded-conical apical cells Carrine Blank apical cells conical-rounded Apical cell that is conical-rounded (conical-blunt). conical apical cell apical cells conical end cells conical terminal cells conical Apical cell that is conical. terminal cells are conical Carrine Blank pointed apical cell pointed at the ends apical cells pointed Apical cell that is pointed (tapered). Carrine Blank pointed terminal cells rounded apical cell club-shaped ends of trichomes and branches end cells spherical terminal cells are rounded rounded apical cells rounded at the ends rounded terminal cell end cells rounded terminal cells rounded apical cells rounded Apical cell that is rounded (blunt). Carrine Blank rounded at the apex rounded end cells rounded apical cell end cells widely spherical end cells widely rounded end cells widened-rounded end cells rounded at apex elongated apical cell Apical cell that is elongated. apical cells long apical cells cylindrical terminal cells elongated apical cells elongated end cells cylindrical apical part elongated Carrine Blank narrowed apical cell apical cells narrowed terminal cells are narrowed end cells narrowed Carrine Blank apical part narrowed Apical cell that is narrowed (has decreased width). terminal cells narrow apical cell develops into nanocytes terminal cells of branches divide often repeatedly in nanocytes Apical cell quality related the developmental transformation of the apical cell into nanocytes. Carrine Blank transforms into nanocytes hyaline apical cell apical cells hyaline Apical cell that is hyaline (spindle-shaped). Carrine Blank elongated-rounded apical cell Apical cell that is elongated-rounded (elongated-blunt). Carrine Blank terminal cells elongated-rounded bluntly pointed apical cell Carrine Blank Pointed apical cell that is bluntly pointed (attenuate). end cells bluntly pointed terminal cells are bluntly pointed apical cells bluntly pointed sharply pointed apical cell apical cells acuminate apical cells sharply pointed Pointed apical cell that is sharply pointed (acuminate). Carrine Blank vacuolized sub-apical cell vacuolized subapical cells Carrine Blank Sub-apical cell quality where the sub-apical cell is vacuolized (has gas vacuoles). subterminal meristematic zones subapical meristematic zones Carrine Blank apical cells are not able to divide Meristematic zone position quality where the position is subterminal. terminal meristematic zones in the apical part sometimes with meristematic zones meristematic zons near the ends of branches Meristematic zone position quality where the position is terminal. Carrine Blank meristematic (mainly apical) zones tapered by apical widening branches dilated terminal portions end cells enlarged trichomes sometimes with slightly widened apical parts end cells widened-rounded end cells widened Tapered trichome quality where the trichome is wider at the apical end, and narrower at the basal end (cuneate-shaped). apical cell is larger than the other ones Carrine Blank tapered by apical narrowing apical cells attenuated Tapered trichome quality where the trichome is narrower at the apical end, and wider at the basal end (attenuate-shaped). trichomes widened at the base Carrine Blank trace elements solution TES Carrine Blank From: Irgens RL. 1977. Meniscus, a new genus of aerotolerant, gas-vacuolated bacteria. Int J Syst Bacteriol 27(1):38-43. The TES, modified from Pfennig’s formula (personal communication), contained (per liter): ZnSO4x7H2O, 0.10 g; MnCl2x4H2O, 0.03 g; H3BO3, 0.3 g; CoCl2x6H20, 0.2 g; CuCl2x2H2O,0.01 g ; NiCl2x6H2O, 0.02 g; Na2MoO4x 2H2O, 0.03g; pH3 to 4. A trace elements solution containing zinc sulfate, manganese chloride, boric acid, cobalt chloride, copper chloride, nickel chloride, and sodium molybdate. Balch vitamin solution Carrine Blank From: Balch WE et al, 1979. Methanogens: reevaluation of a unique biological group. Microbiol Rev 43(2):260. Trace vitamines (contains in milligrams per liter: biotin, 2; folic acid, 2; pyridoxine hydrochloride, 10; thiamine hydrochloride, 5; riboflavin, 5; nicotinic acid, 5; DL-calcium pantothenate, 5; vitamin B12, 0.1; p-aminobenzoic acid, 5; lipoic acid, 5) Also reported in Wolin EA, Wolin MJ, Wolfe RS. 1963. Formation of methane by bacterial extracts. J Biol Chem 238(8):2882-2886. A solution of vitamins containing biotin, folic acid, pyridoxine, thiamine, riboflavin, nicotinic acid, calcium pantothenate, cobalamin (vitamin B12), 4-aminobenzoic acid (p-aminobenzoic acid), and lipoic acid. Used to support growth of methanogenic archaea. Balch trace vitamines prokaryotic metabolite Carrine Blank A chemical entity produced by a prokaryotic cell, as a result of metabolism. organic chemical mixture Carrine Blank Organic compounds or mixtures of organic compounds added to culture media to support growth or metabolism of a microorganism. methane produced by methanogenesis methane production formation of methane methane formed CH4 formation Prokaryotic metabolite where methane is produced as a result of methanogenesis. CH4 formed forming CH4 produce methane CH4 as the product CH4 production methane generated methane as the product methanogens formation of CH4 production of CH4 CH4 generated methane formation Wikipedia:methanogenesis Methanogenesis or biomethanation is the formation of methane by microbes known as methanogens. Organisms capable of producing methane have been identified only from the domain Archaea, a group phylogenetically distinct from both eukaryotes and bacteria, although many live in close association with anaerobic bacteria. The production of methane is an important and widespread form of microbial metabolism. In most environments, it is the final step in the decomposition of biomass. methanogenic CO2 reduction to methane produces CH4 CO2 reduction to CH4 methane as the end product CH4 as the end product methanogenesis produce CH4 methane produced CH4 produced Carrine Blank forming methane production of methane produces methane obligate methanogen Prokaryotic metabolically differentiated cell, specialized for the production of methane. Found only in the archaeal domain of life. obligately methanogenic Carrine Blank undefined organic chemical mixture Carrine Blank A mixture of dry or liquid organic compounds added to culture media to support growth or metabolism of a microorganism. Because the exact composition of the mixture is unknown or uncharacterized, it is referred to as "undefined". tetrathionate reduction Carrine Blank The process in which tetrathionate is reduced to sulfide. microbiological medium ingredient, derived from aqueous extracts of animal tissues or fluids Undefined mixture of complex organic compounds deriving from the aqueous extraction (an extraction using water, such as hot water or steam) of animal organs, animal tissues, or animal secretions. Used in the cultivation of microorganisms. Carrine Blank microbiological medium ingredient, derived from extracts of microbial organisms Undefined mixtures of complex organic compounds deriving from the aqueous extraction (an extraction using water, such as hot water or steam) of microbial (prokaryotic or microbial eukaryotic) cells. Used in the cultivation of microorganisms. Carrine Blank microbiological medium ingredient, derived from oil Undefined mixtures of complex organic compounds derived from hydrophobic substances including lipids, fatty acids, and triacylglycerols, derived from the chemical or physical extraction of oil from an organism, the part of an organism, or a naturally occurring substance (such as crude oil). Carrine Blank microbiological medium ingredient, derived from extracts of proteinaceous material An undefined mixture of complex organic compounds (peptides, sometimes with additional undefined vitamins and cofactors) derived from enzymatic, acid, or base digests of protein from plant or animal sources. Used in the cultivation of microorganisms. Carrine Blank microbiological medium ingredient, derived from chemical hydrolysis of protein Undefined mixtures of complex organic compounds derived (typically amino acids) from the acid hydrolysis of animal or plant protein added to culture media. Carrine Blank microbiological medium ingredient, derived from enzymatic hydrolysis of protein BD Bionutrients Technical Manual (3rd edition revised). Carrine Blank Protein hydrolysates, also called peptones, are the result of the enzymatic hydrolysis of protein. Trypticase peptone Biotrypticase Trademarked commercial name for pancreatic digest of casein (milk protein from cow's milk, Bos taurus), used for the culturing of microorganisms. Reference for Bio-trypticase: "Rapport de Mission au Centre National de l'Elevage et de Recherches Veterinaires de Nouakchott du 12/07 au 02/08/1999"; Institute Senegalais de Recherches Agricoles. Retired name for "peptone pancreatique du caseine"; produced by Bio-Merieux (www.biomerieux.com) or Oxoid (www.remel.com/Microbiology). From BD Bionutrients Technical Manual (3rd edition revised): BBL™ Trypticase™ Peptone is a pancreatic digest of casein and is the primary nitrogen source in Trypticase Soy Broth and Agar. Ash content is 5.7% NaCl content is 0.1% Carrine Blank TrypticaseTM Peptone bio-trypticase Trypticase meat peptone An enzymatic hydrolysis of meat (muscle tissue) proteins from mixed animal (mammalian) sources, used for the culturing of microorganisms. From BD Bionutrients Technical Manual (3rd edition revised): Meat peptones are proteins from animal sources that have been hydrolyzed, or broken down into amino acids and peptides, to provide nitrogen for microorganisms. Meat peptones can be tailored to specific nutritive needs of microorganisms by controlling the quality and origin of the protein, the quality and source of the enzyme used to digest the protein, and the methods used for hydrolysis, concentration and drying the peptone. Peptone manufacture methods are discussed in the section titled Hydrolysis to Hydrolysate. Sources of animal protein include meat from muscle tissue or offal (waste parts, entrails) and gelatin. Muscular tissue and offal are utilized fresh, frozen or dried. Gelatin is extracted by boiling collagen, the fibrous protein found in connective tissue, bone and cartilage. A variety of proteolytic enzymes, or proteases, may be used to accomplish enzymatic hydrolysis of animal protein. Pepsin and trypsin are widely used for animal peptone manufacture. Pepsin is isolated from porcine or other animal stomach. Trypsin, along with chymotrypsin, carboxypeptidase A, carboxypeptidase B, and elastase, are enzymes isolated from animal pancreas. Carrine Blank neopeptone Carrine Blank From BD Bionutrients Technical Manual (3rd edition revised): Bacto™ Neopeptone is an enzymatic digest of protein. Neopeptone contains a wide variety of peptide sizes in combination with vitamins, nucleotides and minerals. Ash content is 6.9% NaCl content is 1.4% An enzymatic digest of meat, used for the culturing of microorganisms. peptone bacterial peptone An enzymatic digest of animal (mammalian) protein (of unspecified orgin), used for the culturing of microorganisms. peptones From BD Bionutrients Technical Manual (3rd edition revised).: Bacto™ Peptone is an enzymatic digest of animal protein. Bacto Peptone was first introduced in 1914 and became the standard Peptone for the preparation of bacteriological culture media. The nutritive value of Bacto Peptone is largely dependent on the amino acid content that supplies essential nitrogen. Bacto Peptone contains only a negligible quantity of proteoses and more complex constituents. Ash content is 6.9% NaCl content is 1.7% Carrine Blank Bacto-peptone Bacto Peptone polypeptone Carrine Blank A mixture of peptones (pancreatic digest of casein and peptic digest of animal/mammalian tissue - meat), used for the culturing of microorganisms. From BD Bionutrients Technical Manual (3rd edition revised).: BBL™ Polypeptone™ Peptone is a mixture of peptones made up of equal parts of pancreatic digest of casein and peptic digest of animal tissue. Polypeptone Peptone includes the high content of amino acids and small polypeptides characteristic of pancreatic digest of casein and the larger polypeptides characteristic of peptic digest of animal tissue. Ash content is 9.7% NaCl content is 2.7% proteose peptone From BD Bionutrients Technical Manual (3rd edition revised). The Bacto™ Proteose Peptones are enzymatic digests of protein. Studies of peptic digests of animal tissue prepared under varying digestion parameters led to the development of Proteose Peptone, Proteose Peptone No. 2 and Proteose Peptone No. 3. Data accumulated during these studies demonstrated that no one peptone is the most suitable nitrogen source for every microbiological application. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone. BiTek™ Proteose Peptone and BiTek Proteose Peptone No. 3 are enzymatic digests of protein, developed to offer alternatives to the Bacto Proteose Peptones for scale-up to production applications. Ash content is 7.8% NaCl content is 4.9% Peptic digest of animal tissue A peptic digest of protein derived from animal (mammalian) tissue, used for the culturing of microorganisms. meat peptic digest Carrine Blank Bacto(TM) Proteose Peptone bacto proteose peptone soy peptone soytone soya peptone bacto soytone A mixed enzymatic digest of flour made of the seed of the soybean (Hordeum vulgare), used for the culturing of microorganisms. Bacto™ Soytone papaic digest of soybean soytone Carrine Blank From BD Bionutrients Technical Manual (3rd edition revised): Enzymeatic digests of soy flour. Soy contains several heat labile protease inhibitors. The most common way of eliminating these factors is to heat or toast the defatted soy beans in a processing plant under controlled conditions. Soy flour, the principle substrate in a soy peptone, is rich in high-quality protein, carbohydrates, calcium and B vitamins. The enzymes used in the digestion of soy flour are typically from animal-free sources or from microorganisms that have been grown in animal-free media. Ash content is 12% NaCl content is 0.2% tryptone Proteose Tryptone Bacto-Tryptone From: Wikipedia:Tryptone Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin. An enzymatic hydrolysate of casein (a milk protein from cow's milk, Bos taurus) made using the hydrolytic enzyme trypsin, used for the culturing of microorganisms. bacto-tryptone Carrine Blank From BD Bionutrients(TM) Techical Manual (3rd edition, revised): Bacto™ Tryptone is a pancreatic digest of casein. It was developed by Difco Laboratories while investigating a peptone particularly suitable for the elaboration of indole by bacteria. It is also notable for the absence of detectable levels of carbohydrates. Ash content is 6.6% NaCl content is 0.0% Bacto Tryptone tryptose From BD Bionutrients Technical Manual (3rd edition revised).: Bacto™ Tryptose is a mixed enzymatic hydrolysate with distinctive nutritional properties. The digestive process of Tryptose results in assorted peptides of higher molecular weight suitable for long-chain amino acid requirements. Ash content is 8.8% NaCl content is 3.2% Carrine Blank A mixed enzymatic hydrolysate of protein (animal meat of unspecified origin), used for the cultivation of microorganisms. casamino acids From BD Bionutrients Technical Manual (3rd edition revised): Bacto™ Casamino Acids is an acid hydrolysate of casein, prepared according to the method described by Mueller and Miller. The method described, reduces the sodium chloride and iron content of the hydrolyzed casein. This hydrolyzed casein, supplemented with inorganic salts, growth factors, cystine, maltose and an optimum amount of iron was used by Mueller and Miller to prepare diptheria toxin. Bacto Casamino Acids duplicate this specially treated hydrolyzed casein. Ash content is 18.3% NaCl content is 12.1% Casein Hydrolysate acid digest of casein Carrine Blank An acid hydrolysate of casein (milk protein from cow's milk, Bos taurus), used for the culturing of microorganisms. crude oil A complex mixture of unrefined petrolium hydrocarbons, pumped from subsurface geologic formations, used in the cultivation of microorganisms. Carrine Blank egg yolk oil Carrine Blank From Wikipedia: Yolk: The yolk of one large egg (50 g total, 17 g yolk) contains approximately: 2.7 g protein, 210 mg cholesterol, 0.61 g carbohydrates, and 4.51 g total fat. All of the fat-soluble vitamins (A, D, E, and K) are found in the egg yolk. Egg yolk is one of the few foods naturally containing vitamin D. The composition (by weight) of the most prevalent fatty acids in egg yolk is typically as follows: Unsaturated fatty acids: Oleic acid, 47% Linoleic acid, 16% Palmitoleic acid, 5% Linolenic acid, 2% Saturated fatty acids: Palmitic acid, 23% Stearic acid, 4% Myristic acid, 1% Oil extracted from yolk of chicken eggs, used in the cultivation of microorganisms. disproportionation of thiosulfate The process by which thiosulfate undergoes disproportionation to sulfate and hydrogen sulfide. Carrine Blank olive oil Carrine Blank Oil extracted from olives (the fruit of Olea europaea), used in the cultivation of microorganisms. From Wikipedia:Olive_oil: Olive oil is a fat obtained from the olive (the fruit of Olea europaea; family Oleaceae), a traditional tree crop of the Mediterranean Basin. The oil is produced by pressing whole olives. Olive oil is composed mainly of the mixed triglyceride esters of oleic acid and palmitic acid and of other fatty acids, along with traces of squalene (up to 0.7%) and sterols (about 0.2% phytosterol and tocosterols). glutamate deaminase assay glutamate dehydrogenase The purpose of this assay is to determine if a microbial isolate is capable of metabolizing glutamate. This enzyme is important for the synthesis of urea and in nitrogen assimilation. Glutamate is deaminated via a hydrolysis reaction to produce 2-oxoglutarate, ammonia, and either NADH or NADPH. Activity can be assayed using colorimetric assays for NADH production. 2-oxoglutarate is also known as alpha-ketoglutarate. Activity could also be determined using a pH sensitive indicator dye. Glutamate dehydrogenase catalyzes the following reaction: L-Glutamate + H2O <-> 2-oxoglutarate + NH3 + NADH/NADHP + H+ L-Glutamate + H2O <-> alpha-ketoglutarate + NH3 + NADH/NADHP + H+ Carrine Blank archaea cell extract An extract made from archaeal cells, used for culturing microorganisms. Carrine Blank bacterial cell extract An extract made from bacterial cells, used for culturing microorganisms. Carrine Blank beef heart infusion From http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM1032&cat=&sec=1: Heart Infusion Broth has been developed to give the same performance characteristics as Brain Heart Infusion (BHI) Broth. However, as bovine brain is a specified risk material, the exclusion of it from the Heart Infusion Broth means that the regulatory requirements when using it are lower. The medium has been developed to give equivalent performance to Brain Heart Infusion but the exclusion of calf brain infusion means that Heart Infusion Broth carries a lower regulatory burden. Simple additions may be made to the medium to make it suitable for the cultivation of yeasts and moulds and for use in blood culture. A highly nutritious infusion medium recommended for the cultivation of streptococci, pneumococci, meningococci and other fastidious organisms. heart infusion Carrine Blank A water extract of bovine (Bos taurus) heart, used in the cultivation of microorganisms. bovine heart infusion brain heart infusion From Wikipedia:Brain_heart_infusion_broth: Brain-heart infusion broth (BHI broth or BHIB) is a highly nutritious general-purpose growth medium for culturing fastidious and nonfastidious microorganisms, such as streptococci, pneumococci and meningococci. It is made by boiling cow or porcine hearts and brains. Boiling releases soluble factors into the broth. The broth can then be turned into powder for easy distribution. BHI broth contains sodium chloride which is used to differentiate enterococci from nonenterococcal group D streptococci. BHI broth is often used in food safety, water safety, and antibiotic sensitivity tests. Carrine Blank Hot water extract of bovine (Bos taurus) or porcine (Sus scrofa) hearts and brains, used in the cultivation of microorganisms. Chlorella cell extract Carrine Blank An extract made from cellular paste from the culture of Chlorella spp., used for the culturing of microorganisms. Chlorella is a microscopic green alga in the Trebouxiophyceae. fecal extract From "Materials and Methods in the Study of Protozoa", pg. 21, by Harold Kirby, University of California Press, 1950: "One part of caecal contents is mixed with 9 parts of Ringer solution, and the mixture strained through a sieve and then through a funnel with a thick pad of absorbent cotten." Carrine Blank A water extract of fecal material derived from some animal (mammal), used in the cultivation of microorganisms. malt extract From BD Bionutrients Technical Manual (3rd edition revised): Bacto™ Malt Extract is the water-soluble portion of malted barley. The extraction process breaks down the polysaccharides into simple sugars. After the malting process is complete, the extract is prepared from the malted barley by cracking the grain in a mill and then extracting the grain with a warm liquor. The resulting “wort” is filtered and evaporated or dried under vacuum. Ash content is 0.3% NaCl content is 0.2% Water-soluble extract of malted (i.e. germinated) seeds of barley (Hordeum vulgare), used for the culturing of microorganisms. Prepared by malting the seed, cracking it (grinding) using a mill, heating it in water, then filtering the extract and drying it. Carrine Blank horse manure extract Carrine Blank manure extract A water extract of horse (Equus caballus) manure, used for the culturing of microorganisms. From "Report of the New York State College of Agriculture at Cornell University, Ithaca, and of the Cornell University Agricultural Experiment Station", v. 25, Part 1, 1913, pg. 431: Manure extract agar. - Prepared as follows: To 100 grams of well-rotted horse manure was added 500 cc. of distilled water. This was allowed to stand for twenty-four hours at room temperature and then filtered. The filtrate was used as a stock solution. meat extract A water extract of animal (mammalian) meat, used for the culturing of microorganisms. From Wikipedia:Meat_extract: Meat extract is highly concentrated meat stock, usually made from beef. Carrine Blank pine needle extract From Jeaon, J-R & Kim, J-Y. 2006. Effects of pine needle extract on differentiation of 3T3-L1 preadipocytes and obesity in high-fat diet fed rats. Biol. Pharm. Bull. 29(10):2111-2115: Pine needles were collected from Kyungbook Province, Republic of Korea, and were extracted using 20 kg of water for 2 h at 80 °C. The extract was filtered, and was then freeze-dried at 40 °C. Pine needle water extract (PNE) was stored at 4 °C until use. A hot water extract of pine needles (the adult leaves of Pinus spp.), used for the culturing of microorganisms. Formed as result of heating pine needles in warm water, filtering the extract and then drying it. Carrine Blank soya extract From Roubos-van den Hil, Dalmas, Nout & Abee. 2009. Soya bean tempe extracts show antibacterial activity against Bacillus cerus cells and spores. J. Appl. MIcrobiol. 109(1):137-145: Fermented soya beans (tempe) and cooked soya beans, were freeze-dried and grounded passing through a 0·5-mm sieve (Ultra Centrifugal Mill ZM 200, Retsch GmbH, Haan, Germany) and were stored at −20°C until further processing. Freeze-dried products were suspended in distilled water (60 g l−1) and stirred with a magnetic stirrer for 3 h at room temperature. The pH was continually checked and adjusted to pH 8·0 with 1 mol l−1 NaOH. To obtain clear supernatants, the soluble extract was obtained by three consecutive centrifugation steps (10 min, 10 000 g, 20°C). Supernatants were freeze-dried and soluble dry matter was stored at −20°C and used as soya bean soluble dry matter in experiments. A water extract of ground beans (the seed) of soya (Glycine max), used for the culturing of microorganisms. Formed by fermenting the beans, heating (cooking) them, filtering the material and freeze dyring it. Next, the material is hydrated, treated with sodium hydroxide, centrifuged and freeze-dried. Soybean extract Soy extract Soya bean extract Carrine Blank yeast extract Yeast extrakt From www.SigmaAldrich.com: A water soluble extract of autolyzed yeast cells. Yeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates and can be used as additive for culture media. A water-soluble extract of autolyzed yeast (Saccharomyces cerevisiae), used for the culturing of microorganisms. Carrine Blank From BD Bionutrients Technical Manual (3rd edition revised): Bacto™ Yeast Extract is one of the most complete and versatile fermentation bionutrients available. It is an important ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia sp., can be isolated from yeast extract Ash content is 11.2% NaCl content is 0.1% Yeastrel beef extract A water extract of beef (Bos taurus) protein, used for the culturing of microorganisms. beef infusion Carrine Blank From BD Bionutrients Technical Manual (3rd edition revised).: Beef Extract is derived from infusion of beef and provides an undefined source of nutrients. Beef Extract is not exposed to the harsh treatment used for protein hydrolysis, so it can provide some of the nutrients lost during peptone manufacture. Beef Extract is a mixture of peptides and amino acids, nucleotide fractions, organic acids, minerals and some vitamins. “Its function can therefore be described as complementing the nutritive properties of peptone by contributing minerals, phosphates, energy sources and those essential factors missing from peptone.” Beef Extract Powder is a meat extract dried to powder form. Bacto™ Beef Extract, Desiccated, is the dried form of Beef Extract paste. Ash content is 9.3% NaCl content is 0.3% Gram stain assay Cell staining assay where a cell staining assay, performed by staining a smear of prokaryotic cells with two stains (Crystal Violet or Safranin). Delineates prokaryotes into two major groups (Gram positive and Gram negative, although results can be ambiguous in some taxa) that results from the different staining of cell wall types. Wikipedia:Gram_staining Gram staining, also called Gram's method, is a method of differentiating bacterial species into two large groups (gram-positive and gram-negative). The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique. Gram staining differentiates bacteria by the chemical and physical properties of their cell walls by detecting peptidoglycan, which is present in a thick layer in gram-positive bacteria.[1] In a Gram stain test, gram-positive bacteria retain the crystal violet dye, while a counterstain (commonly safranin or fuchsine) added after the crystal violet gives all Gram-negative bacteria a red or pink coloring. Carrine Blank zzzz has_participant some [ChEBI] safranin - Safranin is not in ChEBI yet. morphologically distinct prokaryotic cell A prokaryotic cell, or group of cells, having a distinct morphology (shape or size). Carrine Blank prokaryotic colony quality Carrine Blank Physical object quality of a prokaryotic colony. triple sugar iron agar TSI An organic-rich, liquid microbiological culture medium that contains peptones, three sugars (glucose, lactose, and sucrose), ferric iron, thiosulfate, and phenol red (a pH indicator). If the sugars are fermented, the pH indicator will turn red indicating a lowered pH. If hydrogen sulfide is produced, the ferric iron will be reduced to ferrous sulfide, producing a black color. From: http://www.microbelibrary.org/component/resource/laboratory-test/2842-triple-sugar-iron-agar-protocols RECIPE Pancreatic digest of casein USP (see Note) 10.0 g Peptic digest of animal tissue USP (see Note) 10.0 g Glucose 1.0 g Lactose 10.0 g Sucrose 10.0 g Ferrous sulfate or ferrous ammonium sulfate 0.2 g NaCl 5.0 g Sodium thiosulfate 0.3 g Phenol red 0.024 g Agar 13.0 g Distilled water 1,000 mL Note: The following combination of ingredients can substitute for the first two components listed: beef extract, 3.0 g; yeast extract, 3.0 g; and peptone, 20.0 g. Combine ingredients, and adjust the pH to 7.3. Boil to dissolve the agar, and dispense into tubes. Sterilize by autoclaving at 121°C for 15 min. Cool in a slanted position to give a 2.5-cm butt and a 3.8-cm slant. Carrine Blank triple sugar iron agar TSIA Carrine Blank Triple sugar iron agar An organic-rich, solid microbiological culture medium that contains peptones, three sugars (glucose, lactose, and sucrose), ferric iron, thiosulfate, and phenol red (a pH indicator). If the sugars are fermented, the pH indicator will turn red indicating a lowered pH. If hydrogen sulfide is produced, the ferric iron will be reduced to ferrous sulfide, producing a black color. From:http://www.microbelibrary.org/component/resource/laboratory-test/2842-triple-sugar-iron-agar-protocols RECIPE Pancreatic digest of casein USP (see Note) 10.0 g Peptic digest of animal tissue USP (see Note) 10.0 g Glucose 1.0 g Lactose 10.0 g Sucrose 10.0 g Ferrous sulfate or ferrous ammonium sulfate 0.2 g NaCl 5.0 g Sodium thiosulfate 0.3 g Phenol red 0.024 g Agar 13.0 g Distilled water 1,000 mL Note: The following combination of ingredients can substitute for the first two components listed: beef extract, 3.0 g; yeast extract, 3.0 g; and peptone, 20.0 g. Combine ingredients, and adjust the pH to 7.3. Boil to dissolve the agar, and dispense into tubes. Sterilize by autoclaving at 121°C for 15 min. Cool in a slanted position to give a 2.5-cm butt and a 3.8-cm slant. macroscopic optical quality assay An optical quality assay of a single prokaryotic cell, determined by visualization of a liquid culture of a prokaryotic microorganism (i.e., a clonal liquid suspension in a liquid culture medium). Carrine Blank pigmented cell Carrine Blank A prokaryotic cell that is pigmented (in that it has a color imparted by a chemical pigment compound). N-benzoyl-L-leucine peptidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate N-benzoyl-L-leucine-beta-naphthylamide. opalescent turbidity irridescent Carrine Blank irridescence opalescence The optical quality of a liquid culture of clonal prokaryotic organisms, which exhibits opalescent turbidity (refracting different wavelengths of light under white light illumination, or iridescence) after gentle shaking. tyrosine agar Carrine Blank From: http://www.atcc.org/~/media/DF06C418BB3A44189AD1A3CEB59B8D6E.ashx ATCC Medium: 1776 Tyrosine Agar (ISP Medium 7) Glycerol 15.0 g L-Tyrosine 0.5 g L-Asparagine 1.0 g K2HPO4 0.5 g MgSO4x7H2O 0.5 g NaCl 0.5 g FeSO4x7H2O 10.0 mg Trace elemetns solution Ho-Le (see below) 1.0 mL Agar 20.0 g Distilled water 1.0 L Adjust medium for final pH 7.3 +/- 0.1. Autoclave at 121C for 15 minutes. Trace Elements Solution Ho-Le: H3BO3 2.85 g MnCl2x4H2O 1.8g FeSO4 1.36 g Sodium tartrate 1.77 g CuCl2x2H2O 26.9 mg ZnCl2 20.8 mg CoCl2x6H2O 40.4 mg Na2MoO4x2H2O 25.2 mg Distilled water 1.0 L L-tyrosine agar An organic-rich, mineral-salts, solid microbiological culture medium containing glycerol, tyrosine, asparagine, and minerals salts. Used for the growth of Streptoalloteichus. endospore Carrine Blank sporulating spore-forming From Wikipedia:endospore: An endospore is a dormant, tough, and non-reproductive structure produced by certain bacteria from the Firmicute phylum. The name "endospore" is suggestive of a spore or seed-like form (endo means within), but it is not a true spore (i.e., not an offspring). It is a stripped-down, dormant form to which the bacterium can reduce itself. Endospore formation is usually triggered by a lack of nutrients, and usually occurs in Gram-positive bacteria. In endospore formation, the bacterium divides within its cell wall. One side then engulfs the other. Endospores enable bacteria to lie dormant for extended periods, even centuries. Revival of spores millions of years old has been claimed. When the environment becomes more favorable, the endospore can reactivate itself to the vegetative state. Most types of bacteria cannot change to the endospore form. Examples of bacteria that can form endospores include Bacillus and Clostridium. A differentiated prokaryotic cell, found in the Firmicutes, which is specialized for extreme tolerance to desiccation, radiation, and exposure to ultraviolet light. Characterized by thick cell walls. spore-former gas vacuole Wikipedia:bacterial_cell_structure Gas vacuoles are membrane-bound, spindle-shaped vesicles, found in some planktonic bacteria and Cyanobacteria, that provides buoyancy to these cells by decreasing their overall cell density. Positive buoyancy is needed to keep the cells in the upper reaches of the water column, so that they can continue to perform photosynthesis. They are made up of a shell of protein that has a highly hydrophobic inner surface, making it impermeable to water (and stopping water vapour from condensing inside) but permeable to most gases. Because the gas vesicle is a hollow cylinder, it is liable to collapse when the surrounding pressure becomes too great. aerotopes gas vesicles vacuolized gas vacuole gas-vacuolated An intracellular non-membran-bound organelle (cytoplasmic vesicle) made of protein which functions to provide buoyancy to the cell in the water column. Carrine Blank intracellular granule Cytoplasmic part which forms a distinctive granular particle, which may or may not be visible using light microscopy. granule granulated globule granular central granule intracellular globule Wikipedia:granule_(cell biology) In cell biology, a granule is a small particle. It can be any structure barely visible by light microscopy. Carrine Blank granulated magnetosome Wikipedia:magnetosome Magnetosome chains are membranous prokaryotic structures present in magnetotactic bacteria. They contain 15 to 20 magnetite crystals that together act like a compass needle to orient magnetotactic bacteria in geomagnetic fields, thereby simplifying their search for their preferred microaerophilic environments. Each magnetite crystal within a magnetosome is surrounded by a lipid bilayer, and specific soluble and transmembrane proteins are sorted to the membrane. Recent research has shown that magnetosomes are invaginations of the inner membrane and not freestanding vesicles. Magnetite-bearing magnetosomes have also been found in eukaryotic magnetotactic algae, with each cell containing several thousand crystals. Carrine Blank Intracellular membrane-bound organelle that form chains, comprised of magnetic iron minerals that function to orient the cell axis along the Earth's magnetic field lines. Found in magnetotactic prokaryotes. isopolar subsymmetric An isopolar trichome morphology quality where heterocysts are rarely present along the length of the trichome such that the trichome morphology is asymmetric. Carrine Blank xylose minimal medium XeMM From:Humphry DR, George, A, Black GW, Cumming& s SP. 2001. Flavobacterium frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from Antarctica. IJSEM 51:1235-1243. Minimal medium (MM) containing the following (w/v): 0.02% FeSO4, 0.02% MgSO4, 0.075% KNO3, 0.05% K2HPO4 and 0.004% CaCl2. The pH was adjusted to 7.2 with 1 M NaOH or 1M HCl. A mineral-salts, liquid microbiological culture medium comprised of ferrous sulfate, magnesium sulfate, potassium nitrate, potassium phosphate, and calcium chloride, supplemented with 0.5% w/v xylose. Used for the cultivation of Flavobacterium frigidarium. Carrine Blank dispersed thylakoids irregular thylakoids Thylakoid-containing cell where thylakoids are dispered irregularly (asymmetrically) throughout the interior of the cell. dispersed thylakoids Carrine Blank parietal thylakoids peripherally stacked thylakoid radially arranged thylakoids concentrically arranged thylakoids concentric thylakoid membranes Carrine Blank Thylakoid-containing cell where thylakoids are located around the inside periphery of the interior of the cell. parietal thylakoids radial thylakoids radial thylakoids Carrine Blank Thylakoid-containing cell where thylakoids are distributed throughout the inside of the cell and have a radial (like spokes on a wheel) arrangement. greigite magnetosome Carrine Blank Intracellular membrane-bound organelle that form chains, comprised of magnetic iron minerals made of greigite that function to orient the cell axis along the Earth's magnetic field lines. Found in magnetotactic prokaryotes. Wikipedia:magnetosome Magnetotactic bacteria usually mineralize either iron oxide magnetosomes, which contain crystals of magnetite (Fe3O4), or iron sulfide magnetosomes, which contain crystals of greigite (Fe3S4). magnetite magnetosome Intracellular membrane-bound organelle that form chains, comprised of magnetic iron minerals made of magnetite that function to orient the cell axis along the Earth's magnetic field lines. Found in magnetotactic prokaryotes. Wikipedia:magnetosome Magnetotactic bacteria usually mineralize either iron oxide magnetosomes, which contain crystals of magnetite (Fe3O4), or iron sulfide magnetosomes, which contain crystals of greigite (Fe3S4). Carrine Blank granulated cell Carrine Blank A prokaryotic cell which has intracellular granules. gas vacuole quality A prokaryotic cell part quality that has gas vacuoles (gas vesicles). Carrine Blank intracellular carbon storage granule Carrine Blank An intracellular granule that is specialized for the storage of carbon. intracellular nitrogen storage granule An intracellular granule that is specialized for the storage of nitrogen. Carrine Blank intracellular phosphorus storage granule An intracellular granule that is specialized for the storage of nitrogen. Carrine Blank intracellular sulfur granule intracellular sulfur globule Intracellular granule composed of elemental sulfur. sulfur globule sulphur granule Wikipedia:Chromatiaceae The Chromatiaceae are the main family of purple sulfur bacteria. Many members conduct an anoxygenic photosynthesis. They are distinguished from the Ectothiorhodospiraceae by producing sulfur globules and storing them within their cells. sulphur globule Carrine Blank intracellular sulphur globule cell having small gas vacuole small gas vacuole A gas vacuolated cell, were gas vacuoles are small (decreased) in size. small vesicles Carrine Blank small vacuole cell having large gas vacuole large vacuole A gas vacuolated cell, were gas vacuoles are large (increased) in size. Carrine Blank large vesicles large gas vacuole gas vacuolated cell vacuolated vesicles vaculoes Carrine Blank vesiculated A prokaryotic cell that has gas vacuoles (gas vesicles). polyphosphate granule volutin granule metachromatic granule Intracellular phosphorus storage granule composed of polyphosphate. Wikipedia:volutin_granules Volutin granules are an intracytoplasmic (inside the cytoplasm of a cell) storage form of complexed inorganic polyphosphate, the production of which is used as one of the identifying criteria when attempting to isolate Corynebacterium diphtheriae on Löffler's medium. Polyphosphate granules are called metachromatic granules due to their displaying the metachromatic effect; they appear red or blue when stained with the blue dyes methylene blue or toluidine blue. Carrine Blank cyanophycin granule Wikipedia:cyanophycin Cyanophycin, or multi-L-arginyl-poly (L-aspartic acid), is a non-protein, non-ribosomally produced amino acid polymer composed of an aspartic acid backbone and arginine side groups. Carrine Blank Intracellular carbon and nitrogen storage granule composed of cyanophycin macromolecules. yeast water medium Filippini M, Svercel M, Laczko E, Kaech A, Ziegler U & Bagheri HC. 2011. Fibrella aestuarina gen. nov., sp. nov., a filamentous bacterium of the family Cytophagaceae isolated from a tidal flat, and emended description of the genus Rudanella Weon et al. 2008. IJSEM 61:184-189. 0.5% yeast water Carrine Blank An organic-rich, liquid microbiological culture medium consisting of a solution of yeast extract (0.5%) in water. From: Filippini M, Kaech A, Ziegler U & Bagheri HC. 2011. Fibrisoma limi gen. nov., sp. nov., a filamentous bacterium isolated from tidal flats. IJSEM 61:1418-1424. 0.5% yeast water poly-beta-hydroxyalkanoate granule Carrine Blank Wikipedia:polyhydroxyalkanoates Polyhydroxyalkanoates or PHAs are linear polyesters produced in nature by bacterial fermentation of sugar or lipids. They are produced by the bacteria to store carbon and energy. More than 150 different monomers can be combined within this family to give materials with extremely different properties. These plastics are biodegradeable and are used in the production of bioplastics. PHA polyhydroxyalkanoate Intracellular carbon storage granule composed of poly-beta-hydroxyalkanoate. poly-beta-hydroxybutyric acid granule polyhydroxybutyric acid Intracellular carbon storage granule composed of poly-beta-hydroxybutyrate. Carrine Blank PHB polyhydroxybutyrate poly-beta-hydroxybutyrate Wikipedia:polyhydroxybutyrate Polyhydroxybutyrate (PHB) is a polyhydroxyalkanoate (PHA), a polymer belonging to the polyesters class that are of interest as bio-derived and biodegradable plastics. The poly-3-hydroxybutyrate (P3HB) form of PHB is probably the most common type of polyhydroxyalkanoate, but other polymers of this class are produced by a variety of organisms: these include poly-4-hydroxybutyrate (P4HB), polyhydroxyvalerate (PHV), polyhydroxyhexanoate (PHH), polyhydroxyoctanoate (PHO) and their copolymers. starch granule starch Intracellular carbon storage granule composed of starch. Carrine Blank coursely granulated course granules large granule Carrine Blank Granulated cell, where the granual size is coarse (has an increased size). finely granulated fine granules Carrine Blank Granulated cell, where the granule size is fine (has a decreased size). numerously granulated 2 numerous granules Granulated cell, where there are many granules (an increased number) per cell. Carrine Blank singularly granulated 1 single granule solitary granules Granulated cell, where there are one granule per cell. Carrine Blank singular granule central endospore Carrine Blank An endospore that forms in the middle of the cell. lateral endospore Carrine Blank An eEndospore that forms laterally to the long axis of a cell. subterminal endospore Carrine Blank An endospore that forms near the ends (or the poles) of the cell, but not at the extreme ends of the cell. terminal endospore Carrine Blank An endospore that forms at the pole (end) of the cell. filaments Amorphously described generic term for filament- or pili-like structures. tuft of filaments pili-like structures Carrine Blank akinete Carrine Blank A differentiated prokaryotic cell, found in the Nostocales (Cyanobacteria), which play a role in dormancy (such as overwintering in lake sediments). Characterized by thick cell walls. From Wikipedia:akinete: An akinete is a thick-walled dormant cell derived from the enlargement of a vegetative cell. It serves as a survival structure. It is a resting cell of cyanobacteria and unicellular and filamentous green algae. Under magnification, akinetes appear thick walled with granular-looking cytoplasms. akinete, adjacent to heterocyte akinetes adjacent to heterocysts Rippka, Castenholz & Herdman, 2001, in Bergeys Manual of Systematic Bacteriology, v. 1, 2nd ed., pg 563. An akinete which is proximally located to a heterocyst. paraheterocytic Carrine Blank akinete, distal to heterocyte Carrine Blank apoheterocytic An akinete that isdistally located from the heterocytes. May occur multiply in chains that, in the absence of combined nitrogen, are initiated at a site that is equidistant from two intercalary or terminal heterocysts. akinetes distant from heterocysts Rippka, Castenholz & Herdman, 2001, in Bergeys Manual of Systematic Bacteriology, v. 1, 2nd ed., pg 563. yeast extract tryptone medium Carrine Blank YT YT broth An organic-rich, liquid microbiological culture medium containing pancreatic digest of casein, yeast extract, and sodium chloride. Used for the cultivation of Escherichia coli. From: 2x Yeast Extract Tryptone (2X YT) Medium (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use 2x YT Medium is used for cultivating recombinant strains of Escherichia coli. Principles of the Procedure Peptone and yeast extract provide the necessary nutrients and cofactors required for excellent growth of E. coli. Sodium chloride is included to provide a suitable osmotic environment. 2x YT Medium formula per Liter: Pancreatic digest of casein 16.0 g Yeast extract 10.0 g Sodium chloride 5.0 g pH 7.0 ± 0.2 yeast extract tryptone agar Carrine Blank An organic-rich, solid microbiological culture medium containing pancreatic digest of casein, yeast extract, and sodium chloride. Used for the cultivation of Escherichia coli. From: 2x Yeast Extract Tryptone (2X YT) Medium (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use 2x YT Medium is used for cultivating recombinant strains of Escherichia coli. Principles of the Procedure Peptone and yeast extract provide the necessary nutrients and cofactors required for excellent growth of E. coli. Sodium chloride is included to provide a suitable osmotic environment. 2x YT Medium formula per Liter: Pancreatic digest of casein 16.0 g Yeast extract 10.0 g Sodium chloride 5.0 g Agar pH 7.0 ± 0.2 YT agar Todd-Hewitt medium Carrine Blank Todd-Hewitt broth Todd Hewitt Broth From: http://www.neogen.com/Acumedia/pdf/ProdInfo/7161_PI.pdf Intended Use Todd Hewitt Browth is used for the cultivation of steptococci and other fastidious microorganisms. Formula / Liter: Heart infusion (dehydrate) 3.1 g Yeast enriched peptone 20 g Dextrose 2 g Sodium chloride 2 g Disodium phosphate 0.4 g Sodium carbonate 2.5 g Final pH: 7.8 +/- 0.2 at 25˚C. Directions: 1. Dissolve 30 g of the medium in one liter of purified water. 2. Heat with frequent agitation to compeltely dissolve the medium. 3. Autoclave at 121 ˚C for 15 minutes. An organic-rich, liquid microbiological culture medium containing heart infusion (beef heart infusion), yeast enriched peptone (Biosate peptone), dextrose (D-glucose), sodium chloride, disodium hydrogenphosphate, and sodium carbonate. Used for the cultivation of Streptococci. sheath sheath Carrine Blank mucilaginous slime mucilage Castenholz, 2001, in Bergey's Manual of Systematic Bacteriology, vol 1, 2nd ed., pg. 474. An "envelope" outside of the outer membrane.... variously called the sheath, glycocalyx, or capsule, or depending on the consistency, may be referred to as gel, mucilage, or slime. Many sheaths show a microfibrillar substructure. The sheaths of cyanobacteria are predominantly composed of polysaccharide, but in some strains >20% of the weight may consist of polypeptides. ... pigments may accumulate and mask the color of the cells. capsule extracellular polymeric material Part of the glycocalyx, or envelope of a prokaryotic cell, that lies outside the outer membrane. gel gelatinous matrix mucous extracellular polymeric substance ensheathed EPS exopolysaccharide laminated sheath layered sheath striated steath lamillated sheath A sheath having a layered, laminated (i.e., laminar) structure. striated sheathed structure Carrine Blank pigmented sheath A sheath that is pigmented (contains pigmented compounds). colored sheath Carrine Blank proteinaceous sheath A sheath comprised largely of protein. Carrine Blank tryptone glucose extract agar Carrine Blank From: Tryptone Glucose Extract Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Tryptone Glucose Extract Agar is used for cultivating and enumerating microorganisms in water and dairy products. Principles of the Procedure Nutrients, including amino acids, carbon compounds, carbohydrates, minerals and trace substances, are supplied by the tryptone, beef extract and dextrose. Agar is the solidifying agent in Tryptone Glucose Extract Agar. Formulae Difco™ Tryptone Glucose Extract Agar Approximate Formula* Per Liter Beef Extract.................................................................. 3.0 g Tryptone...................................................................... 5.0 g Dextrose (Glucose)....................................................... 1.0 g Agar.......................................................................... 15.0 g pH 7.0 ± 0.2 Difco™ m TGE Broth Approximate Formula* Per Liter Beef Extract.................................................................. 6.0 g Tryptone.................................................................... 10.0 g Dextrose (Glucose)....................................................... 2.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2 An organic-rich, solid microbiological culture medium containing beef extract, tryptone, and glucose. Used for cultivation of microorganisms from water and dairy products. TGE agar pseudopeptidoglycan-based cell wall pseudomurein Wikipedia:pseudopeptidoglycan Pseudopeptidoglycan (also known as pseudomurein) is a major cell wall component of some archaea that differs from bacterial peptidoglycan in chemical structure, but resembles eubacterial peptidoglycan in morphology, function, and physical structure. The basic components are N-acetylglucosamine and N-acetyltalosaminuronic acid (peptidoglycan has N-acetylmuramic acid instead), which are linked by β-1,3-glycosidic bonds. Lysozyme, a host defense mechanism, is ineffective against organisms with pseudopeptidoglycan cell walls. Lysozyme can break β-1,4-glycosidic bonds to degrade peptidoglycan; however, pseudopeptidoglycan has β-1,3-glycosidic bonds, rendering lysozyme useless. Carrine Blank Cell wall composed a polymer of N-acetylglucosamine and N-acetyltalosaminuronic acid (2-amino-2-deoxy-L-taluronic acid), linked by beta-1,3-glycosidic bonds. Found in the archaeal domain of life. Biosate peptone yeast enriched peptone From BD Bionutrients Technical Manual (3rd edition revised): BBL™ Biosate™ Peptone is a mixed hydrolysate comprised of 65% pancreatic digest of casein and 35% yeast extract. Ash content is 7.7% NaCl content is 0.3% BBL(TM) Biosate(TM) Peptone A mixed hydrolysate of pancreatic digest of casein (65%) and yeast extract (35%). Biosate is a Trademark of BD. Carrine Blank teichoic acid-based cell wall Carrine Blank Wikipedia:teichoic acid Teichoic acids (cf. Greek τεῖχος, teīkhos, "wall", more specifically a fortification wall, as opposed to τοῖχος, toīkhos, a regular wall) are bacterial polysaccharides of glycerol phosphate or ribitol phosphate linked via phosphodiester bonds. Teichoic acids are found within the cell wall of Gram-positive bacteria such as species in the genera Staphylococcus, Streptococcus, Bacillus, Clostridium, Corynebacterium and Listeria, and appear to extend to the surface of the peptidoglycan layer. Teichoic acids are not found in Gram-negative bacteria. They can be covalently linked to N-acetylmuramic acid of the peptidoglycan layer, to the lipids of the cytoplasmic membrane, or to a terminal D-alanine in the tetrapeptide crosslinkage between N-acetylmuramic acid units. Teichoic acids that remain anchored to lipids are referred to as lipoteichoic acids, while teichoic acids that are covalently bound to peptidoglycan are referred to as wall teichoic acids. Cell wall composed of polysaccharides of glycerol phosphate or ribitol phosphate linked via phosphodiester bonds, found in the peptidoglycan cell walls of Gram-positive bacteria. TBBP medium An organic-rich, liquid medium containing animal and plant peptones and sodium chloride. Supplemented with sheep blood, yeast extract, bacitracin, and polymyxin B. Used for the cultivation of Capnocytophaga spp. Carrine Blank From: Mashimo PA, Yamamoto Y, Nakamura M & Slots J. 1983. Selective recovery of oral Capnocytophaga spp. with sheep blood agar containing bacitracin and polymyxin B. J Clin Microbiol 17:187-91. TBBP medium consists of 4% Trypticase soy agar (BBL Microbiology Systems, Coceysville, Md.), 5% sheep blood, 0.1 % yeast extract, 50 micrograms of bacitracin per mL, and 100 micrograms of polymyxin B per mL. TBBP agar Carrine Blank From: Mashimo PA, Yamamoto Y, Nakamura M & Slots J. 1983. Selective recovery of oral Capnocytophaga spp. with sheep blood agar containing bacitracin and polymyxin B. J Clin Microbiol 17:187-91. TBBP medium consists of 4% Trypticase soy agar (BBL Microbiology Systems, Coceysville, Md.), 5% sheep blood, 0.1 % yeast extract, 50 micrograms of bacitracin per mL, and 100 micrograms of polymyxin B per mL. An organic-rich, solid medium containing animal and plant peptones and sodium chloride. Supplemented with sheep blood, yeast extract, bacitracin, and polymyxin B. Used for the cultivation of Capnocytophaga spp. diazocyte Carrine Blank A prokaryotic differentiated cell capable of nitrogen fixation, found in Trichodesmium species (Cyanobacteria). Diazocytes are mostly found in the center cells of Trichodesmium filaments. From Sandh, Xu & Bergman, 2012, Diazocyte development in the marine diazotrophic cyanobacterium Trichodesmium. Microbiology 158:345-352.: The establishment of non-diazotrophic cultures of the filamentous marine cyanobacterium Trichodesmium erythraeum IMS101 enabled the first detailed investigation of the process leading to the development of its unique nitrogen-fixing cell type, the diazocyte. extracellular mineral precipitates Carrine Blank Crystalline, microcystalline, nanocrystalline, or amorphous inorganic chemical precipitates that form outside the cell as a result of the local presence of the cell or the activities of the cell. extracellular iron precipitates Carrine Blank extracellular iron Crystalline, microcystalline, nanocrystalline, or amorphous inorganic chemical precipitates of iron minerals that form outside the cell as a result of the local presence of the cell or the activities of the cell. extracellular sulfur globule Crystalline, microcystalline, nanocrystalline, or amorphous inorganic chemical precipitates of elemental sulfur that forms outside the cell as a result of the local presence of the cell or the activities of the cell. Produced by Ectothiorhodospiraceae. extracellular sulphur granule extracellular sulfur globule Wikipedia:Ectothiorhodospiraceae The Ectothiorhodospiraceae are a family of purple sulfur bacteria, distinguished by producing sulfur globules outside of their cells. Carrine Blank fimbriae Carrine Blank A pilus, that is thin and short. fimbriated Wikipedia:Fimbria_(bacteriology) In bacteriology, a fimbria [(plural fimbriae); also referred to as a pilus (plural pili) by some scientists] is an appendage composed of curlin proteins that can be found on many Gram-negative and some Gram-positive bacteria that is thinner and shorter than a flagellum. This appendage ranges from 3-10 nanometers in diameter and can be up to several micrometers long. Fimbriae are used by bacteria to adhere to one another and to adhere to animal cells and some inanimate objects. A bacterium can have as many as 1,000 fimbriae. Fimbriae are only visible with the use of an electron microscope. They may be straight or flexible. fimbria SWC medium A dilute organic-containing, liquid microbiological culture medium containing tryptone, yeast extract, beef extract, and acetate, in a base of half-strength artifical seawater. From: Irgens Rl, Suzuki I, Staley JT. 1989. Gas vacuolate bacteria obtained from marine waters of Antarctica. Curr Microbiol 18:261-5. SWC contained (per liter of 1/2 strength artificial seawater [ASW]): tryptone, 0.5 g; yeast extract, 0.5 g; beef extract, 0.2 g; sodium acetate, 0.2 g. The pH was adjusted to 7.6 with a dilute solution of NaOH. ASW, 1/2 strength, contained (per liter): NaCl, 12.0 g; MgSO4x7H2O, 3.5 g; MgCl2x6H2O, 2.6 g; CaCl2x2H2O, 0.55 g; KCl, 0.35 g. Carrine Blank tetrazolium reduction assay An assay for the ability of a microorganism to reduce a compound that contains a tetrazoleum moiety. Tetrazolium compounds are redox indicators of microbial oxidation and reduction, and hence microbial respiration. Carrine Blank multiply flagellated 1 Flagellated cell where the cell has more than one flagellum. numerous flagella Carrine Blank polytrichous flagellated cell flagellated Carrine Blank Prokaryotic cell which has either a bacterial-type flagellum (or flagella) or a periplasmic flagellum. singly flagellated 1 Carrine Blank Flagellated cell where the cell has only one flagellum. monotrichous cell http://microbeonline.com/bacterial-flagella-structure-importance-and-examples-of-flagellated-bacteria/ Monotrichous: Single polar flagellum e.g. Vibrio cholerae (Mneomonics: Mono means one) single flagellum single flagella monopolar flagella polar flagellum monopolar flagellum Flagellated cell where the cell has a single flagellum located at the end (pole) of the cell. monotrichous monotrichous flagella Carrine Blank amphitrichous cell 2 Carrine Blank amphitrichous Flagellated cell where the cell has two flagella, each located at the ends (poles) of the cell. http://microbeonline.com/bacterial-flagella-structure-importance-and-examples-of-flagellated-bacteria/ Amphitrichous: Single flagellum at both ends e.g. Alcaligenes faecalis (Mneomonics: Remember: the characteristics of Amphibians: live both in land and water) lophotrichous cell 2 Carrine Blank tuft of polar flagella Flagellated cell where the cell has multiple flagella, located in a tuft at the end (pole) of the cell. bundles of flagella http://microbeonline.com/bacterial-flagella-structure-importance-and-examples-of-flagellated-bacteria/ Lophotrichous: Tuft of flagella at one or both ends e.g. Spirilla polar flagella polar bundle of flagella polar tuft of flagella lophotrichous monopolar polytrichous flagella cell having periplasmic flagella axial fibrils axial filaments periplasmic fibrils Flagellated cell where the cell has multiple flagella, each located in tufts in the periplasmic space. http://microbewiki.kenyon.edu/index.php/Spirochaeta The morphology and cellular structure of Spirochaeta spp. (and most other Spirochetes) is unique among prokaryotes. The cells are helical in shape and consist of an outer membrane, axial filaments (ultrastructurally similar to bacterial flagella), and a protoplasmic cylinder. The outer membrane or the outer sheath surrounds all of the structures including the axial filaments and the protoplasmic cylinder. The protoplasmic cylinder is the cell body of the organism. It is coiled and is composed of the cytoplasm, the nuclear region and the peptidoglycan-cytoplasmic membrane complex. The axial filaments are usually called the periplasmic flagella but are also known as periplasmic fibrils, axial fibrils and endoflagella. Carrine Blank periplasmic flagella endoflagella peritrichous cell 2 lateral flagella peritrichously http://microbeonline.com/bacterial-flagella-structure-importance-and-examples-of-flagellated-bacteria/ Peritrichous: Flagella surrounding the cell, e.g. Typhoid bacilli (Mneomonic: Remember Periphery) Flagellated cell where the cell has multiple flagella, all located at the periphery of the cell. peritrichous Carrine Blank tumbling motility Wikipedia:Listeria Prokaryotic motility that is characterized microscopically by end-over-end tumbling and employs a flagellum. This type of motility is typical of Listeria. Carrine Blank tumbling heterocyte heterocystous heterocytous Carrine Blank heterocysts From Wikipedia:heterocyst: Heterocysts are specialized nitrogen-fixing cells formed during nitrogen starvation by some filamentous cyanobacteria, such as Nostoc punctiforme, Cylindrospermum stagnale, and Anabaena sphaerica. They fix nitrogen from dinitrogen (N2) in the air using the enzyme nitrogenase, in order to provide the cells in the filament with nitrogen for biosynthesis. Nitrogenase is inactivated by oxygen, so the heterocyst must create a microanaerobic environment. A prokaryotic differentiated cell capable of nitrogen fixation, found in the filamentous Nostocales (Cyanobacteria). Heterocytes (which differentiate into heterocysts) have thick cell walls which exclude oxygen in the air and allow nitrogen fixation to occur. Are terminally differentiated cells, incapable of carrying out photosynthesis. intercalary heterocyte bipored heterocyte Carrine Blank A heterocyte which develops at the middle or near the end (but not the end) of a filament (trichome). intercalary heterocyst From Rippka, Castenholz & Herdman, 2001, in Bergeys Manual of Systematic Bacteriology, v. 1, 2nd ed., pg 563.: In the absence of combined nitrogen (ammonium or nitrate), 5-10% of the vegetative cells differentiate into heterocysts. These specialized cells are the sites of aerobic nitrogen fixation and may occupy terminal or intercalary positions in the trichomes. lateral heterocyte terminal heterocyte terminal heterocyst Carrine Blank From Rippka, Castenholz & Herdman, 2001, in Bergeys Manual of Systematic Bacteriology, v. 1, 2nd ed., pg 563.: In the absence of combined nitrogen (ammonium or nitrate), 5-10% of the vegetative cells differentiate into heterocysts. These specialized cells are the sites of aerobic nitrogen fixation and may occupy terminal or intercalary positions in the trichomes. A heterocyte which develops at the end of a filament (trichome). unipored heterocyte hormogonium Prokaryotic differentiated cell specialized for asexual reproduction comprised of short chains of motile filaments, found in the Nostocales, Stigonematales/Stigonemataceae, and Oscillatoriales (Cyanobacteria). Hormogonia are shorter than the typical length of vegetative filaments. Carrine Blank hormogonia From Wikipedia:hormogonium: Hormogonia are motile filaments of cells formed by some cyanobacteria in the order Nostocales and Stigonematales. They are formed during asexual reproduction in unicellular, filamentous cyanobacteria some contain heterocysts and akinetes. SWC-m medium SWCm salts Carrine Blank A dilute organic-containing, liquid microbiological culture medium containing tryptone, yeast extract, beef extract, and acetate, in a base of full-strength artifical seawater. From: Irgens Rl, Suzuki I, Staley JT. 1989. Gas vacuolate bacteria obtained from marine waters of Antarctica. Curr Microbiol 18:261-5. SWC-m contained (per liter of full strength ASW): KH2PO4, 0.01 g; ferric citrate, 0.001 g; NH4Cl, 0.4 g; yeast extract, 0.4 g; beef extract, 0.4 g; tryptone, 0.4 g; vitamins, 10 mL; trace elements solution (TES), 2.0 mL; carbon source, 0.2 g; pH 7.0. The following macromolecular carbon sources were tested for hydrolysis on plates: starch, 1.0%; tributyrin, 1.0%; chitin, 1.0%; casein, 0.5%. xxxxxxx From: Irgens RL. 1977. Meniscus, a new genus of aerotolerant, gas-vacuolated bacteria. Int J Syst Bacteriol 27(1):38-43. The TES, modified from Pfennig’s formula (personal communication), contained (per liter): ZnSO4x7H2O, 0.10 g; MnCl2x4H2O, 0.03 g; H3BO3, 0.3 g; CoCl2x6H20, 0.2 g; CuCl2x2H2O,0.01 g ; NiCl2x6H2O, 0.02 g; Na2MoO4x 2H2O, 0.03g; pH3 to 4. xxxxxxx From: Staley JT. 1981. The genera Prosthecomicrobium and Ancalomicrobium. In: Starr MP, Stolp H, Truper HG, Balows A,Schlegel HG, (eds). The prokaryotes. Berlin: Springer-Verlag. Pg 68. Vitamin Solution B12 0.1 mg Biotin 2 mg Calcium pantothenate 5 mg Folic acid 2 mg nicotinamide 5 mg pyridoxine HCl 10 mg riboflavin 5 mg thiamine HCL 5 mg Add distilled water up to 1 L and sotre in 4˚C in a dark container. conjugative pili Pilus involved in the transfer of DNA from one bacterium (the donor) to another (the recipient). Wikipedia:pilus Conjugative pili allow the transfer of DNA between bacteria, in the process of bacterial conjugation. They are sometimes called "sex pili", in analogy to sexual reproduction, because they allow for the exchange of genes via the formation of "mating pairs". Perhaps the most well-studied is the F pilus of Escherichia coli, encoded by the F plasmid or fertility factor. Carrine Blank SWC-m agar Carrine Blank A dilute organic-containing, liquid microbiological culture medium containing tryptone, yeast extract, beef extract, and acetate, in a base of full-strength artifical seawater. From: Irgens Rl, Suzuki I, Staley JT. 1989. Gas vacuolate bacteria obtained from marine waters of Antarctica. Curr Microbiol 18:261-5. Agar slants containted 1.5 % agar, agar plates contained 2.0 % agar, and soft agar tubes contained 0.2 % agar. SWCM agar From: Irgens Rl, Suzuki I, Staley JT. 1989. Gas vacuolate bacteria obtained from marine waters of Antarctica. Curr Microbiol 18:261-5. SWC-m contained (per liter of full strength ASW): KH2PO4, 0.01 g; ferric citrate, 0.001 g; NH4Cl, 0.4 g; yeast extract, 0.4 g; beef extract, 0.4 g; tryptone, 0.4 g; vitamins, 10 mL; trace elements solution (TES), 2.0 mL; carbon source, 0.2 g; pH 7.0. The following macromolecular carbon sources were tested for hydrolysis on plates: starch, 1.0%; tributyrin, 1.0%; chitin, 1.0%; casein, 0.5%. xxxxxxx From: Irgens RL. 1977. Meniscus, a new genus of aerotolerant, gas-vacuolated bacteria. Int J Syst Bacteriol 27(1):38-43. The TES, modified from Pfennig’s formula (personal communication), contained (per liter): ZnSO4x7H2O, 0.10 g; MnCl2x4H2O, 0.03 g; H3BO3, 0.3 g; CoCl2x6H20, 0.2 g; CuCl2x2H2O,0.01 g ; NiCl2x6H2O, 0.02 g; Na2MoO4x 2H2O, 0.03g; pH3 to 4. xxxxxxx From: Staley JT. 1981. The genera Prosthecomicrobium and Ancalomicrobium. In: Starr MP, Stolp H, Truper HG, Balows A,Schlegel HG, (eds). The prokaryotes. Berlin: Springer-Verlag. Pg 68. Vitamin Solution B12 0.1 mg Biotin 2 mg Calcium pantothenate 5 mg Folic acid 2 mg nicotinamide 5 mg pyridoxine HCl 10 mg riboflavin 5 mg thiamine HCL 5 mg Add distilled water up to 1 L and sotre in 4˚C in a dark container. distinctly shaped colony Carrine Blank http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol elevated A colony that has a distinct morphology manifested in its 2D or 3D shape. physically distinct colony http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol A prokaryotic colony that has distinct physical characteristics (qualities). Carrine Blank colony having distinct spatial pattern A colony having distinct physical quality that manifests in the spatial pattern of the elements of the colony. Carrine Blank colony having distinct optical quality A prokaryotic colony that manifests in how light (visible electromagnetic radiation) passes through the colony. Carrine Blank texturally distinct colony Carrine Blank A colony with a texture that manifests in the appearance of the surface characteristics. satellite colonies Carrine Blank satellite colonies satellite behavior A prokaryotic colony where small colonies surround larger colonies on an agar (or other solid) surface. http://www.yourdictionary.com/satellite#americanheritage Satellite colonies occur when tiny colonies are found to surround a larger central colony. satellites dense center colony Carrine Blank A colony with distinct spatial pattern where the center part of the colony is tight (densely compacted) and the outer part of the colony is sparse (not densely compacted). dense center diffuse center colony dry colony dry colony Carrine Blank A colony having distinct physical quality that is dry (has a brittle, friable texture that breaks apart). friable dry dull colony Carrine Blank not shiny A colony with distinct optical quality that is dull (not shiny, with a low saturation) in its appearance. dull dull colony granular center colony granular center colony A colony with a structure that has a grainy, or granular, appearance in its center. fine granulation granular granular centers Carrine Blank glistening colony moist shiny surface wet shining aspect glistening shiny A colony with distinct EM radiation quality that has a wet appearance. glistening colony Carrine Blank shining glossy mucoid colony http://www.medilexicon.com/medicaldictionary.php?t=19041 Carrine Blank mucoid mucous A colony with a structure that is mucoid (has a sticky appearance due to the presence of exopolysaccharide capsular material). mucoid colony rough colony rough colony A colony with a texture that is rough (i.e. not smooth). Carrine Blank rough surface smooth colony smooth smooth-surfaced smooth colony A colony with a texture that is smooth. Carrine Blank dendritic colony dendritic colony Carrine Blank A colony with a shape that has raised, branching features starting in the center of the colony, with an appearance that resembles veins or branches of a tree. veined dendiritic wrinkled colony rugose A colony with a texture that is wrinkled (has a shriveled appearance). shriveled Carrine Blank wrinkled colony cloudy colony A colony with distinct optical quality that is cloudy (semi-translucent or semi-opaque, whitish in color). semi-translucent cloudy colony cloudy Carrine Blank semi-opaque iridescent colony A colony having a distinct process quality where the colony is iridescent (appears to change color as the angle of illumination changes). iridescent http://en.wikipedia.org/wiki/Iridescence opalescent irridescent colony Carrine Blank pigmented colony A colony with a structure that contains coloration due to the presence of pigment compounds that absorb particular wavelengths of light. coloured colored Carrine Blank pigmented colony opaque colony Carrine Blank opaque colony A colony with distinct optical quality that is opaque (where light does not pass through). opaque bovine albumin Carrine Blank From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Bovine Albumin contains bovine albumin fraction V, 0.2% in 0.85% saline solution. A blood medium ingredient comprised of bovine (Bos taurus) albumin (a mixture of protein derived from blood). Used in the cultivation of microorganisms. translucent colony translucent colony Carrine Blank translucent semi-transparent almost clear A colony with distinct optical quality that is translucent, where much light is able to pass through it without absorption of particular wavelengths of light. transparent colony transparent Carrine Blank clear transparent colony A colony with distinct optical quality that is completely or nearly completely clear or transparent, where nearly all light is able to pass through it. anaerobic respiration, using sulfate as electron acceptor The process of anaerobic respiration, where sulfate is reduced to sulfite, tetrathionate, thiosulfate, elemental sulfur, or hydrogen sulfide. Carrine Blank pyrazinamidase activity A hydrolase activity, which hydrolyzes pyrazinamide (a drug used to treat tuberculosis) into pyrazinoic acid (the active agent against tuberculosis). Pyrazinamidase catalyzes the following reaction: Pyrazinamide + H2O <=> pyrazinoic acid (pyrazine-2-carboxylic acid) + NH3. E.C. 3.5.1.b15 Carrine Blank anaerobic respiration, using elemental sulfur as electron acceptor The process of anaerobic respiration, where elemental sulfur reduced to hydrogen sulfide. Carrine Blank aerobic respiration, using hydrogen sulfide as electron donor The process of aerobic respiration, where hydrogen sulfide is oxidized (using oxygen) to elemental sulfur, thiosulfate, tetrathionate, sulfite, or sulfate. Carrine Blank aerobic respiration, using elemental sulfur as electron donor The process of aerobic respiration, where elemental sulfur is oxidized (using oxygen) to thiosulfate, tetrathionate, sulfite, or sulfate. Carrine Blank anaerobic respiration, using thiosulfate as electron acceptor The process of anaerobic respiration, where thiosulfate is reduced to elemental sulfur or hydrogen sulfide. Carrine Blank anaerobic respiration, using sulfite as electron acceptor Carrine Blank The process of anaerobic respiration, where sulfite is reduced to tetrationate, thiosulfate, elemental sulfur, or hydrogen sulfide. chymotrypsin assay using BTEE Chymotrypsin assay that uses the substrate N-Benzoyl-L-tyrosine ethyl ester (BTEE). Chymotrypsin activity will cleave the substrate, releasing N-Benzoyl-L-tyrosine. The presence of this produce is measured by recording the increase in absorbance at 256 nm. Carrine Blank chymotrypsin assay using N-benzoyl-Phe-pNA Carrine Blank N-benzoyl-D-L-phenylalanine 2-naphthylamide Chymotrypsin assay that uses the substrate N-benzoyl-D-L-phenylalanine-2-naphthylamide at pH 7.1. Trypsin activity will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color. trypsin assay using NA Uses the substrate N-benzoyl-D-L-arginine 2-naphthylamide at pH 8.5. Trypsin activity will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. N-benzoyl-arginine-2-naphthylamide Carrine Blank N-benzoyl-DL-arginine-2-naphthylamide alanyl alanine arylamidase activity Carrine Blank An aminopeptidaseeptidase activity which cleaves the chromogenic substrate L-alanyl-L-alanine-2-naphthylamide. alanyl phenylalanyl proline arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-alanyl-L-phenylalanyl-L-proline-2-naphthylamide. Carrine Blank glutamyl glutamic acid arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-glutamyl-L-glutamic acid-2-naphthylamide. Carrine Blank glutamyl glycine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-glutamyl-glycine-2-naphthylamide. glutamyl glycyl arginine arylamidase activity Carrine Blank GGAA An aminopeptidase activity which cleaves the chromogenic substrate L-glutamyl-glycyl-L-arginine-2-naphthylamide. glutaryl phenylalanine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate glutaryl-L-phenylalanine-2-naphthylamide. glycyl phenylalanine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate glycyl-L-phenylalanine-2-naphthylamide. Carrine Blank glycyl proline arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate glycyl-L-proline-2-naphthylamide. glycyl tryptophan arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate glycyl-L-tryptophan-2-naphthylamide. Carrine Blank glycyl glycine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate glycyl-glycine-2-naphthylamide. leucyl glycine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-leucyl-L-glycine-2-naphthylamide. Carrine Blank seryl tyrosine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-seryl-L-tyrosine-2-naphthylamide. Carrine Blank N-carbobenzoyl-glycyl-glycyl-L-arginine peptidase activity An aminopeptidase activity which cleaves the chromogenic substrate N-carbobenzoyl-glycyl-glycyl-arginine-beta-naphthylamide. Carrine Blank irregular cell highly irregular irregular Carrine Blank Prokaryotic cell where the shape is irregular. irregular shape microscopic optical quality assay Carrine Blank An optical quality assay of a single prokaryotic cell, determined by visualization under a microscope. N-benzoyl-L-valyl-glycyl-L-arginine-4-methoxy peptidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate N-benzoyl-L-valyl-glycyl-4-methoxy-beta-naphthylamide. asymmetrical colony margin irregular margin irregular form http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol A prokaryote colony where the shape of the colony margin is asymmetrical (i.e. irregular). Carrine Blank irregular edge diffuse colony A colony with distinct spatial pattern where the colony is diffuse (sparse, where the colony is very thin). diffuse colony Carrine Blank diffuse asymmetrical filamentous colony asymmetrical filamentous colony irregular filamentous A filamentous colony with a shape that is fuzzy, or filamentous and where the colony margin is asymmetrical (i.e. irregular). http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol undulate colony undulate margin Carrine Blank A colony with a shape that is undulate (wave-like) and where the colony margin is asymmetrical (i.e. irregular). http://www.microbelibrary.org/component/resource/laboratory-test/3114-colony-morphology?limit=0&limitstart=0 undulate colony http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol undulate symmetrical colony margin A prokaryotic colony where the shape of the colony margin is symmetrical (i.e. regular). regular margin regular edge http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol Carrine Blank symmetrical colony margin curled colony http://www.ehow.com/list_6938999_types-bacteria-colonies.html curled http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol A colony with a shape that has a curled edge, and where the colony margin is symmetrical (i.e. regular). wavy margin Carrine Blank curled colony entire colony Carrine Blank http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol entire colony entire A colony with a shape which is circular (round), and where the colony margin is symmetrical (i.e. is regular). erose colony Carrine Blank http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol erose A colony with a shape which is erose (i.e. has a notched, toothed, or indented appearance) and where the colony margin is symmetrical (i.e. regular). erose colony symmetrical filamentous colony A filamentous colony with a shape that is fuzzy, or filamentous and where the colony margin is symmetrical (i.e. regular). regular filamentous Carrine Blank symmetrical filamentous colony http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol lobed colony lobate margin A colony with a shape that is lobed and where the colony margin is asymmetrical (i.e. irregular). lobel colony http://www.microbelibrary.org/component/resource/laboratory-test/3114-colony-morphology?limit=0&limitstart=0 Carrine Blank http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol circular colony round http://www.microbelibrary.org/component/resource/laboratory-test/3114-colony-morphology?limit=0&limitstart=0 http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol A colony with a shape that is circular (round). circular colony is round circular colony Carrine Blank effuse colony effuse Carrine Blank effuse colony A colony with distinct spatial pattern where the colony is effuse (i.e. thin, unlocalized without defined margins, with a tendancy to spread). filamentous colony http://www.microbelibrary.org/component/resource/laboratory-test/3114-colony-morphology?limit=0&limitstart=0 A colony with a shape that is fuzzy, or filamentous. filamentous colony Carrine Blank http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol fried-egg colony fried-egg colony Fallon RJ. 1967. Mycoplasmas and their role as rodent pathogens. Lab Anim 1:43. DOI: 10.1258/002367767781006794. http://medical-dictionary.thefreedictionary.com/Fried+Egg+Appearance A colony having distinct physical quality that has differential density. The center part of the colony is opaque and granular, while the surrounding perifery is flat and transulcent, giving the colony the appearance of a fried egg. This shape is typical of Mycoplasma species, and is due to colonies having a thicker center than edge. This appearance is typical of older (mature) colonies of Mycoplasma. fried egg fried eggs Carrine Blank colony having distinct electromagnetic (EM) radiation quality Carrine Blank A colony having distinct physical quality that manifests in the electromagnetic (EM) radiation appearance of the colony. mulberry colony mulberry appearance mulberry colony Carrine Blank Fallon RJ. 1967. Mycoplasmas and their role as rodent pathogens. Lab Anim 1:43. DOI: 10.1258/002367767781006794. A colony having distinct physical quality that is shaped like a mulberry (acinar), sunken, and opaque with little or no pale periphery. Is typical of young colonies of Mycoplasma species that will develop into colonies that have a fried egg appearance when more mature. mulberry punctiform colony A colony with a shape that is very tiny, like a point (where colonies may have a diameter of less than 1 mm). punctiforme colony http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol punctiform Carrine Blank rhizoidal colony http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol irregular rhizoid form http://www.microbelibrary.org/component/resource/laboratory-test/3114-colony-morphology?limit=0&limitstart=0 rhizoid colony rhizoid form A colony having distinct physical quality that is rhizoidal (has margins or edges that resemble the roots of a tree, radiating out from the center of the colony). Carrine Blank rhizoidal rhizoid raised rhizoid growth spindle colony A colony with a shape that is spindle-shaped (i.e. is thickest in the middle, tapering to both ends). Carrine Blank http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol spindle colony fusiform spindle-shaped crateriform colony A colony with a shape that has a depression (or crater) in the center of the colony, and thus has a 2D profile that is cup-shaped. cup-shaped crateriform colony crateriform dimpled collapsed center crater-shaped Carrine Blank flat colony A colony with a shape that is flat (has a flat profile). flat colony flat elevation http://www.microbelibrary.org/component/resource/laboratory-test/3114-colony-morphology?limit=0&limitstart=0 Carrine Blank http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol raised colony http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol raised colony Carrine Blank A colony with a shape that is raised, or elevated from the surface of the agar. pulvinate colony pulvinate colony pulvinate elevation Carrine Blank http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol A colony with a shape that is pulvinate (is strongly elevated with a domed profile being shaped like a cushion). pulvinate http://www.microbelibrary.org/component/resource/laboratory-test/3114-colony-morphology?limit=0&limitstart=0 convex colony http://www.microbelibrary.org/component/resource/laboratory-test/3114-colony-morphology?limit=0&limitstart=0 convex Carrine Blank A colony with a shape that has a convex, or domed, profile. convex colony http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol convex elevation domed umbonate colony Carrine Blank raised at the centres raised at the center umbonate colony umbonate elevation A colony having distinct physical quality that is umbonate (having a mushroom-like profile with a higher center and a lower margin). http://www.microbelibrary.org/component/resource/laboratory-test/3136-colony-morphology-protocol http://www.microbelibrary.org/component/resource/laboratory-test/3114-colony-morphology?limit=0&limitstart=0 raised in the center prokaryotic cell autofluorescence The optical assay of a single prokaryotic cell, having the quality of autofluorescence when illuminated with a particular wavelength of light. Carrine Blank blue-green autofluorescence blue-green fluorescence Autofluorescence of a prokaryotic cell that is blue-green in nature, occurring when the cells are illuminated with radiation at 420 nm. Occurs due to the presence of a particular fluorescent compound in the cell (e.g., Factor 420, also known as Coenzyme F420). Carrine Blank lipase C10 activity Carrine Blank A lipase that hydrolyzes C10 compounds. Assayed using the chromogenic substrate 5-bromo-3-indoxyl-caprate (5-bromo-3-indolyl decanoate; blue-caprate). lipase C14 activity A lipase that hydrolyzes C14 compounds. Assayed using the chromogenic substrate 2-naphthyl myristate. Carrine Blank lipase C8 activity A lipase that hydrolyzes C8 compounds. Assayed using the chromogenic substrate 2-naphthyl caprylate. Carrine Blank coccobacillus Carrine Blank coccobacillary coccobacilli sub-coccoidal Wikipedia:Coccobacillus A coccobacillus (plural coccobacilli) is a type of bacterium with a shape that is intermediate between cocci (spherical bacteria) and bacilli (rod-shaped bacteria). Coccobacilli are therefore in essence very short rods which may be mistaken for cocci. Haemophilus influenzae, Gardnerella vaginalis, and Chlamydia trachomatis are coccobacilli. Aggregatibacter actinomycetemcomitans is a gram-negative coccobacillus that is prevalent in subgingival plaques. Acinetobacter strains may grow on solid media as coccobacilli. Bordetella pertussis is a gram-negative coccobacillus responsible for causing whooping cough. A prokaryotic cell, being a bacillus where the long axis of the cell is only slightly longer than the short axis of the cell. ellipsoidal short rods subspherical oval ovoid subcoccoidal coccobacillus spirillum Wikipedia:Spirillum Spirillum in microbiology refers to a bacterium with a cell body that twists like a spiral. It is the third distinct bacterial cell shape type besides coccus and bacillus cells. Carrine Blank A prokaryotic cell, where the long axis of the cell twists in a spiral. spiral coiled helical spirillum helix wavy prokaryotic cell having distinct cell arrangement aggregates Prokaryotic cells and the physical relationships between the cells (whether they are physically attached, or connected, to one another). Carrine Blank aggregations paired cells 2 Carrine Blank doublets diploid A multicellular relationship of prokaryotic cells where two cells are attached to one another in pairs. pairs morphologically differentiated filament part A filament of prokaryotic cells, where the parts of the filament are morphologically differentiated. Carrine Blank sarcina 8 12 16 sarcinae Carrine Blank A multicellular relationship of prokaryotic cells where cells are attached to one another in multiples of four (8,12, or 16). This morphology forms as a result of cell division occurring in three planes. sarcinoid cell cluster cell clusters A multicellular relationship of prokaryotic cells where cells are attached to one another end-to-end and side-to-side to form clumps or sheets after dividing. This morphology forms as a result of cell division taking place in multiple planes. clusters packet-like Carrine Blank packets clumps cell chain Carrine Blank chains A multicellular relationship of prokaryotic cells where cells are attached to one another end-to-end to form chains. Forms when cell division occurs in one plane and cells become detached after cell division takes place. short chains tetrad 4 tetrads Carrine Blank A multicellular prokaryotic morphological quality where cells are attached to one another in multiples of four. Forms when cell division occurs in two planes at right angles to one another. unicellular prokaryote A prokaryotic cellularity where cells are isolated and singular, where the daughter cells detach from one another after cell division and septation occurs. unicellular Carrine Blank singly singular streptobacillus Wikipedia:Streptobacillus Streptobacillus is a genus of aerobic, gram-negative facultative anaerobe bacteria, which grow in culture as rods in chains. A multicellular relationship of prokaryotic cells where the shape of the cell is elongated (forming a bacillus, or short rod) and the cells are attached to one another end-to-end to form chains. streptobacilli Carrine Blank streptococcus Wikipedia:Streptococcus Streptococcus is a genus of spherical Gram-positive bacteria belonging to the phylum Firmicutes and the lactic acid bacteria group. Cellular division occurs along a single axis in these bacteria, and thus they grow in chains or pairs, hence the name—from Greek στρεπτος streptos, meaning easily bent or twisted, like a chain (twisted chain). Contrast this with staphylococci, which divide along multiple axes and generate grape-like clusters of cells. Most streptococci are oxidase- and catalase-negative, and many are facultative anaerobes. streptococci Carrine Blank A multicellular relationship of prokaryotic cells where the shape of the cells is round (forming a coccus) and the cells are attached to one another end-to-end to form chains. staphylococcus A multicellular relationship of prokaryotic cells where the shape of the cells is round (forming a coccus) and the cells are attached to one another end-to-end and side-to-side to form clumps or clusters. staphylococci Wikipedia:Staphylococcus Staphylococcus (from the Greek: σταφυλή, staphylē, "grape" and κόκκος, kókkos, "granule") is a genus of Gram-positive bacteria. Under the microscope, they appear round (cocci), and form in grape-like clusters. Carrine Blank differentiated filament branching branching of filaments Carrine Blank branching branches A differentiated cyanobacterial filament where the filaments are branched. branched filaments false-branched filaments commonly false branched false branched false branching growing usually at first parallel with original trichome, later diverging Carrine Blank false branching Differentiated filament branching, where filaments have "false" branches. Initiated by the interruption of a trichome between two vegetative cells, either at the location of a necridic cell or a heterocyte. Later on, one or both ends of the interrupted trichomes grow aside from the axis of the main trichome. Found in the Oscilaltoriales and Nostocales and Stigonematales. pseudobranched single false branching falsely branched false ramification pseudobranchings lateral false branches http://www.merriam-webster.com/dictionary/false%20branching A branched arrangement of the cells of certain filamentous bacteria and algae resulting from a slipping of the end of one cell past that of another following cell division, from continued growth of the free end of a trichome through the sheath in various blue-green algae, or esp. from continued growth of parts of a filament separated by one or more intervening dead cells or by heterocysts. pseudobranchings adpressed with each other and only in the upper parts divaricate true-branched filaments Differentiated filament branching, where filaments have "true" branches. Formed when one cell occasionally divides longitudinally (with the cell division plane parallel to the plane of the filament axis) or obliquely with respect to the main axis of the filament. Branches remain attached to the main trichome. true branching true-branching true branches true branching Carrine Blank branching true true branched true-branching filaments true branches grow initially perpendicular to the mother trichome true-branched branches arise after lengthwise cell division diplobacillus A multicellular prokaryotic morphological quality where the shape of the cells are elongated (i.e. bacillus or rod-shaped) and two cells are attached to one another in pairs. Carrine Blank http://medical-dictionary.thefreedictionary.com/diplobacillus A short, rod-shaped organism occurring in pairs, joined end-to-end; diplobacterium. diplobacilli diplococcus dumbbell dumbbell-shaped diplococci Wikipedia:Diplococcus A diplococcus (plural diplococci) is a round bacterium (a coccus) that typically occurs in the form of two joined cells. Examples are gram-negative Neisseria sp., and gram-positive Streptococcus sp. and Staphylococcus sp.. Its name comes from diplo, meaning double, and coccus, meaning berry. A multicellular prokaryotic morphological quality where the shape of the cells are round and two cells are attached to one another in pairs. Carrine Blank erect filaments A filament orientation quality where filaments are lying upright (perpendicular) to a substrate or surface. filaments erect Carrine Blank prostrate filaments Carrine Blank filaments prostrate A filament orientation quality where filaments are lying along (parallel to) a substrate or surface. straight filament filaments straight Carrine Blank A filament shape where the filament has a straight appearance (is not bent, curved, coiled, or torulose). trichomes straight curved filament filaments flexous filaments curved Carrine Blank A filament shape where the filament has a curved appearance along a gradual angle (not straight or bent). filaments flexuous branches waved branches arcuated filaments arcuated trichomes curved trichomes arcuate coiled filament Carrine Blank coiled filaments trichomes coiled A filament shape where the filament has a regularly coiled (helical) appearance. filaments coiled bent filament filaments bent filaments crooked filaments bent in the middle A filament shape where the filament has an appearance of being bent at an angle. Carrine Blank torulose filament filaments roughened trichomes torulose A torulose filament shape where the filament has a non-uniformly beaded appearance, where parts of the filament are swollen and constricted. Carrine Blank filaments torulose http://www.thefreedictionary.com/torulose Adj. 1. torulose - of a cylindrical or ellipsoid body; swollen and constricted at intervals moniliform filament Carrine Blank A filament shape where the filament has a uniformly regular, beaded appearance. Wikipedia: Moniliform Having a form resembling a string of beads, where the component parts or segments are more or less uniform in size and are spherical or rounded in shape. filaments resembling a string of beads trichomes moniliform filaments moniliform trichome part Carrine Blank Any consituent part of a prokaryotic heteropolar filament. basal heterocyte A trichome part that is comprised of the terminal (basal) cell in a trichome, which has differentiated to form a heterocyte (heterocyst). Found in trichomes with apical-basal polarity that have an erect positionality. Carrine Blank tight filament coiling filaments forming tight, regular spirals A coiled filament shape where the shape of the coiling is tight, having increased coiling. densely coiled Carrine Blank tight filament coiling loose filament coiling loose filament coiling filaments slightly coiled A coiled filament shape where the shape of the coiling is loose, having decreased coiling. filaments loosely coiled Carrine Blank irregular filament coiling trichomes irregularly coiled irregular filament coiling trichomes irregularly waved trichomes irregularly screw-like or spirally coiled A coiled filament shape where the shape of the coiling is irregular. Carrine Blank irregularly arranged coiled filaments irregularly coiled coils irregular filaments irregularly spirally coiled regular filament coiling Carrine Blank filaments screw-like coiled A coiled filament shape where the shape of the coiling is regular. helical trichomes coils regular regular filament coiling screw-like coiled cyanobacterial thylakoid Carrine Blank Bacterial thylakoid that is present in Cyanobacteria. differentiated cyanobacterial filament part Carrine Blank A cyanobacterial filament part (e.g. apical cell, meristematic zone) which is differentiated. trichome_differentiation distinctively shaped prokaryotic cell Carrine Blank Prokaryotic cell having a distinctive shape. bacillus rod-shaped rod-like bacillus rods Carrine Blank Wikipedia:Bacillus_(shape) The word bacillus (plural bacilli) may be used to describe any rod-shaped bacterium, and such bacilli are found in many different taxonomic groups of bacteria. However, the name Bacillus, capitalized and italicized, refers to a specific genus of bacteria. The name Bacilli, capitalized but not italicized, can also refer to a more specific taxonomic class of bacteria that includes two orders, one of which contains the genus Bacillus. Bacilli are usually solitary, but can combine to form diplobacilli, streptobacilli, and palisades. bacilli rod shaped A prokaryotic cell, where one axis (the long axis, the length) of the cell is longer than the other axis (the short axis, the width) of the cell. coccus Carrine Blank coccoidal strongly lobed spherical polygonal-rounded spheroids isodiametric coccoid sphere-shaped semi-globose Wikipedia:Coccus Coccus (plural cocci or coccuses) can be used to describe any bacterium that has a spherical shape. It is one of the three distinct types of bacteria shapes, the other two being bacillus (rod-shaped) and spirillum (spiral-shaped) cells. Coccus is an English loanword of a Neolatin noun, which in turn stems from the Greek masculine noun kokkos (κόκκος) meaning "berry". lobed multi-lobed coccus globose sphaerical spheroidal lobes spheres A prokaryotic cell that is spherical with no apparent long axis. cocci cylindrical cell Carrine Blank Wikipedia:Cylinder_(geometry) A cylinder (from Greek κύλινδρος – kulindros, "roller, tumbler") is one of the most basic curvilinear geometric shapes, the surface formed by the points at a fixed distance from a given line segment, the axis of the cylinder. The solid enclosed by this surface and by two planes perpendicular to the axis is also called a cylinder. The surface area and the volume of a cylinder have been known since deep antiquity. cylindrical barrel-shaped barreliform A prokaryotic cell, being a bacillus where the long axis of the cell is much longer than the short axis of the cell. discoid disk disk shaped plate-shaped discoidal Carrine Blank disc A flattened prokaryotic cell where the shape is partially spherical. discoid disc-shaped flattened cell flat flattened A prokaryotic cell where one part of the cell has been made flat so that the shape of the cell is asymmetrical or irregular. flattened cell Carrine Blank compressed pear-shaped cell pear shaped flask shaped flask A prokaryotic cell where one end end of the cell are wider than the middle (pear shaped). flask-like pear-like Carrine Blank pearshaped pear-shaped flaskshaped polygonal cell polygonal shape polygonal Carrine Blank A prokaryotic cell where the surface of the cell is not rounded but is polygonal (has angular edges). prosthecate cell Carrine Blank A prokaryotic cell where the cell has appendages or stalks that are extensions of the cell surface (including the cell wall and cell membrane). appendaged stalked prosthecate Wikipedia:Prosthecate_bacteria Prosthecate bacteria are a non-phylogenetically related group of Gram-negative bacteria that possess appendages, termed prosthecae. These cellular appendages are neither pili nor flagella, as they are extensions of the cellular membrane and contain cytosol. One notable group of prosthecates is the genus Caulobacter. cuboidal cell Carrine Blank square A prokaryotic cell where the surface of the cell is polygonal and the angles of the cell meet at right angles forming a box. Wikipedia:Haloquadratum Haloquadratum ("salt square") is a genus of the family Halobacteriaceae. The first species to be identified in this group, Haloquadratum walsbyi, is highly unusual since its cells are shaped like flat, square boxes. cuboidal pyramidal cell Carrine Blank triangles pyramidal A prokaryotic cell where the surface of the cell is polygonal and the angles of the cell meet at angles that are less than 90 degrees giving the cell a triangular or pyramidal appearance. triangular hemispherical Carrine Blank A sub-cylindrical, flattened prokaryotic cell where the shape is hemispherical (flattened on one side of the sphere). hemisphaerical hemispherical nanocytes Prokaryotic cells that are very small formed as a result of rapid cell division. Carrine Blank diphtheroid cell club-shaped shape regularity diptheroid Carrine Blank diphtheroid A prokaryotic cell, being a bacillus where one one end is wider and more rounded than the other end (i.e. being club-shaped, or cudgel-shaped). The cannonical morphology of Corynebacterium diphtheriae. corneform vibrioid cell crooked Carrine Blank Wikipedia:Vibrio Vibrio is a genus of Gram-negative bacteria possessing a curved rod shape (comma shape), several species of which can cause foodborne infection, usually associated with eating undercooked seafood. A prokaryotic cell, being a bacillus where the elongated axis of the cells is curved (but not spiral). cresent-shaped vibrioid curved multiseriate filament 2 2 multiserial Seong-Joo L & Golubic S. 1998. Multi-trichomous cyanobacterial microfossils from the Mesoproterozoic Gaoyuzhuang Formation, China: Paleoecological and taxonomic implications. Lethaia 31:169-184. Multi-trichomous filamentous cyanobacteria of the genera Schizothrix and Microcoleus are the most commonly reported constructive elements in modern microbial mat ecosystems. .... A few specimens within the studied assemblage contain two to several parallel rows of evenly spaced internal bodies within a tube (Fig. 5C, D0, corresponding to a multi-trichomous arrangement. Castenholz, 2001, in Bergey's Manual of Systematic Bacteriology, v. 1, 2nd ed., pg. 473. polyseriate filaments with 2-3 joined trichomes multi-seriate multi-trichomous Cyanobacterial filament with one trichome in the sheath, where the trichome is greater than two cells in width. Arises as a result of cell division occuring in two planes,one perpendicular to the axis of the trichome and one parallel to the axis of the trichome. multitrichomous Carrine Blank uniseriate filament 1 1 uniserial trichomes uniseriate Cyanobacterial filament with one trichome in the sheath, where the trichome is one cell in width. Arises as a result of cell division occuring in only one plane. sheaths envelop always only one trichome Carrine Blank sheaths containing a single trichome trichomes in pairs in one sheath Wiktionary:uniseriate Having one line or series; uniserial, unbranched distinctively sized prokaryotic cell Prokaryotic cell having a distinctive size (width). Carrine Blank filterable cell filterable pass through a filter Carrine Blank A distinctively-sized prokaryotic cell, where the size is small enough to pass through a filter with a defined pore size. filterability pass through filters filtered nanophytoplankton Carrine Blank From: Wikipedia: Nanophytoplankton Nanophytoplankton are particularly small phytoplankton with sizes between 2 ­and 20 µm. They are the autotrophic part of nanoplankton. Like other phytoplankton, nanophytoplankton are microscopic organisms that obtain energy through the process of photosynthesis and must therefore live in the upper sunlit layer of ocean or other bodies of water. These microscopic free-floating organisms, including algae, protists, and cyanobacteria, fix large amounts of carbon which would otherwise be released as carbon dioxide. Photosynthetic prokaryotes, or single-celled eukaryotes, that have a cell size between 2 and 20 micrometers in diameter. picoplankton Carrine Blank From: Wikipedia: Picoplankton Picoplankton is the fraction of plankton composed by cells between 0.2 and 2 μm that can be either : Some species can also be mixotrophic. Picoplankton are responsible for the most primary productivity in oligotrophic gyres, and are distinguished from nanoplankton and microplankton. Because they are small, they have a greater surface to volume ratio, enabling them to obtain the scarce nutrients in these ecosystems. Note that the SI prefix pico- is used quite loosely here, as nanoplankton and microplankton are only 10 and 100 times larger, respectively. Prokaryotes or single-celled eukaryotes that have a cell size between 0.2 and 2 micrometers in diameter. heterotrophic picoplankton Prokaryotes or single-celled eukaryotes that have a cell size between 0.2 and 2 micrometers in diameter and that are not photosynthetic, but rather use organic carbon as a source of energy. From: Wikipedia: Heterotrophic_picoplankton Heterotrophic picoplankton is the fraction of plankton composed by cells between 0.2 and 2 μm that do not perform photosynthesis. Carrine Blank photosynthetic picoplankton picophytoplankton From: Wikipedia: Photosynthetic_picoplankton Photosynthetic picoplankton is the fraction of the phytoplankton performing photosynthesis composed by cells between 0.2 and 2 µm (picoplankton). It is especially important in the central oligotrophic regions of the world oceans that have very low concentration of nutrients. Prokaryotes or single-celled eukaryotes that have a cell size between 0.2 and 2 micrometers in diameter and that are photosynthetic (use light as a source of energy). Carrine Blank picocyanobacteria Prokaryotes or single-celled eukaryotes that have a cell size between 0.2 and 2 micrometers in diameter, that are photosynthetic (use light as a source of energy), and that are members of the genus Prochlorococcus and iin some members of the genus Synechococcus (both Cyanobacteria). Carrine Blank From: Scanlan DJ et al. 2009. Ecological genomics of marine picocyanobacteria. Microbiol Mol Biol Rev 73(2):249-299. Marine picocyanobacteria of the genera Prochlorococcus and Synechococcus numerically dominate the picophytoplankton of the world ocean, making a key contribution to global primary production. septate filaments septa septate Carrine Blank Filament septation, where septa separating the individual cells that make up the filament are present. septum multi-trichomous filament 1 1 Cyanobacterial filament one or more than one trichome in the sheath, with the trichomes oriented parallel to one another, where each trichome is one cell in width. Arises as a result of cell division mostly occurring in only one plane, and occasionally occurring in a second plane. filaments contain one or more trichomes placed together and more or less parallel within one sheath filaments composed of one to more parallel arranged trichomes filaments single or many together sheaths enveloping one (rarely 2) trichomes sheaths containing 1 to several trichomes Carrine Blank one or more trichomes per filament tapered trichome taper A heteropolar trichome that is tapered (has a larger cell diameter at one end of the filament than the other). Carrine Blank tapering cells tapered pleomorphic cell A prokaryotic cell that is irregular in shape, where the shapes of the cells are irregular and variable (pleomorphic). Cell shapes may be variable in size and shape, depending on the environmental conditions. pleiomorphic polymorphic pleomorphic polymorphous Carrine Blank ZoBell starch agar An organic-rich, mineral-salts, solid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Supplemented with starch. Used for the cultivation of heterotrophic marine microorganisms. Starch agar Carrine Blank ZoBell starch agar From: Barbeyron T, L'Haridon S, Corre E, Kloareg B & Potin P. 2001. Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. IJSEM 51:985-997. The strain was assayed for amylase activity using starch at a concentration of 0.2 % (w/v) in ZoBell agar or in ZoBell Phytagel plates. indole assay Wikipedia:Indole_test indole test Carrine Blank Indole formed indole test positive tryptophanase IND Kovac's reagent Metabolic test for tryptophanase [L-tryptophan indole-lyase (deaminating)] activity. Measures the formation of indole from tryptophan using the enzyme Tryptophanase. Indole is detected by the addition of Kovac's reagent (isoamy alchohol, para-dimethylaminobenzaldehyde, and concentrated HCl). Indole forms a complex with para-dimethylaminobenzaldehyde, resulting a red color. A negative result is yellow. In some API tests, James' reagent (a proprietary mixture of HCl, water, and 2-methoxy-4-dimethylamino benzaldehyde) is used instead of Kovac's reagent, as it has a longer shelf life. Tryptophanase catalyzes the following reaction: Tryptophan + H2O <=> indole + pyruvate + ammonia TRP Kovacs reagent indole indole production prokaryotic cell wall lysis susceptibility Prokaryotic cell wall quality that defines how susceptible the cell wall is to lysis when the cell is placed in the presence of an environment with an altered chemical composition (detergent, water, hypotonic solution). Carrine Blank susceptibile to lysis by detergent sensitive to SDS lyse rapdily in SDS susceptible to lysis by SDS cell wall is SDS sensitive Carrine Blank easily lysed by detergent sensitive to lysis by detergent SDS-sensitive susceptible to lysis by sodium dodecyl sulfate Disintegrated by SDS lysis in SDS lysis with SDS sensitive to sodium dodecyl sulfate lysed on addition of SDS sensitive to lysis by SDS lysed by sodium dodecyl sulfate lyse in SDS lyse completely in SDS lyse completely in sodium dodecyl sulfate SDS-soluble Susceptibility to lysis by detergent where the cell shows increased susceptibility toward lysis by detergent. lysis by SDS sensistive to lysis by sodium dodecyl sulfate lysed in SDS lysed with sodium dodecyl sulfate lysed by SDS susceptibile to lysis by hypotonic solution lysis occurs in hypotonic solution lysed by hypotonic solution osmotically fragile Carrine Blank lyse in hypotonic distilled water lyse rapidly in hypotonic solution Susceptibility to lysis by hypotonic solution where the cell shows increased susceptibility toward lysis by hypotonic solution. lysed in low osmolal solutions susceptible to lysis by hypotonic solution lyse in hypotonic distilled water susceptible to lysis by water lyse immediately in distilled water lyse after transfer into demineralized water lyse in distilled water lyse rapidly in water lyse completely in distilled water lyse in water Carrine Blank sensitive to distilled water lysis in distilled water lysed in H2O lysed in distilled water lysed on addition of freshwater lyse rapidly in distilled water lysed in water Susceptibility to lysis by water where the cell shows increased susceptibility toward lysis by water. DNA Guanosine and Cytidine Content The molar percentage of guanosine and cytidine nucleobases ([molG+molC]/[molG+molC+molA+molT)) in the DNA of a prokaryotic chromosome and/or plasmid. mol% guanine plus cytosine mol% GC DNA base composition mol% G+C G+C content of the DNA moles guanine+cytosine mol % G+C GC content G+C mol % Carrine Blank mol % guanine plus cytosine G+C content DNA mol G+C content guanine plus cytosine content guanine-plus-cytosine content nitrate reduction The process in which nitrite is reduced to more reduced nitrogen compounds, including nitrite, nitric oxide, nitrous oxide, and dinitrogen. Carrine Blank gliding motility twitching gliding bacterial gliding Cell gliding, characterized by bacterial movement that does not involve use of a flagellum. Gliding is typically observed when a particular genus or species of microorganism encounters a surface. twitching motility Wikipedia:bacterial_gliding Carrine Blank nitrite reduction The process in which nitrite is reduced to more reduced nitrogen compounds, including nitric oxide, nitrous oxide, and dinitrogen. Carrine Blank anaerobic respiration, using tetrathionate as electron acceptor Carrine Blank The process of anaerobic respiration, through the reduction of tetrathionate to thiosulfate, elemental sulfur, or hydrogen sulfide. Berkefeld W filter A gravity-driven water filter made by the British Berkefeld® company, with a wider pore size W (for Wenig). Pore sizes average 0.45 microns in diameter. Carrine Blank prokaryote cytopathogenicity cytopathogenic Carrine Blank Pathogen role of a pathogenic prokaryotic cell. http://www.merriam-webster.com/dictionary/cytopathogenic Berkefeld V filter Carrine Blank A gravity-driven water filter made by the British Berkefeld® company, with a narrow pore size V (for Viel). Pore sizes average 0.38 microns in diameter. This is the most common filter size used to study the filterability of microorganisms. intracellular storage of carbon Single-organism metabolic process, where the prokaryotic microorganism uses enzymes to synthesize a storage molecule (usually a polymer) inside the cell that is used to store carbon. Carrine Blank microbiological reactivity to lymphatic materials assay An assay to determine if a particular microbial taxon or isolate has reactivity towards blood, blood plasma, or blood cells (including clotting). Carrine Blank intracellular storage of nitrogen Single-organism metabolic process, where the prokaryotic microorganism uses enzymes to synthesize a storage molecule (usually a polymer) inside the cell that is used to store nitrogen. Carrine Blank egg yolk emulsion From: McClung-Toabe Agar (Atlas, The Handbook of Microbiological Media for the Examination of Food, 2nd ed., pgs 221-222) Egg Yolk Emulsion, 50%: Composition per 100.0 mL: Chicken egg yolks 11 Whole chicken egg 1 NaCl (0.9% solution) 50.0 mL Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100 dilution of saturated mercuric chloride solution for 1 min. Crack eggs and separate yolks from whites. Mix egg yolks with 1 chicken egg. Measure 50.0 mL of egg yolk emulsion and add to 50.0 mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize. Warm to 45-50˚C. Carrine Blank An emulsion of chicken egg and chicken egg yolks, created by heating an homogenized mixture of egg and egg yolks with sodium chloride. erythrocyte hemadsorption assay hamadsorption haemadsorb A microbiological assay for binding to blood cells, where the cell of a microorganism adheres to red blood cells. hemadsorb HAd haemadsorption http://medical-dictionary.thefreedictionary.com/hemadsorption\ hemadsorption /he·mad·sorp·tion/ (hem″ad-sorp´shun) the adherence of red cells to other cells, particles, or surfaces.hemadsor´bent Carrine Blank hemolysis assay A microbiological assay of lysis of blood cells, where the assay is for cytolysis of red blood cells. Hemolysis assays are carried out using some formulation of blood agar, and the assay involves determination of whether lysis has occured and the nature of the lysis. Carrine Blank hemolysis serum digestion liquifaction of coagulated serum alpha-hemolysis assay alpha-haemolysis alpha hemolysis green hemolysis incomplete hemolysis viridands-type hemolysis Wikipedia:hemolysis_(microbiology) Alpha-haemolysis occurs when an organism secretes alpha-haemolysin, a protein which forms a pore in the membranes of red blood cells. This leads to the formation of colonies on blood agar that have a dark greenish appearance. The green color is caused by peroxidation of hemoglobin (presence of biliverdin). A hemolysis assay for the process of alpha-hemolysis, where a protein secreted by a microorganism results in the formation of a pore in the membranes of erythrocytes (red blood cells) and thus causing the cells to lyse. Alpha hemolysis partially breaks down the red blood cells leaving a greenish color due to the presence of biliverdin, a by-product of hemoglobin. partial hemolysis Carrine Blank viridans type haemolysis green haemolysis incomplete haemolysis alpha-hemolysis partial haemolysis beta-hemolysis assay beta hemolysis Carrine Blank beta-haemolysis complete haemolysis A hemolysis assay for the process of beta-hemolysis, where a protein (beta-haemolysin) secreted by a microorganism results in the hyrolysis of sphingomyelin in erythrocytes (red blood cells) and thus causing the cells to lyse. Beta-hemolysis breaks down the red blood cells and hemoglobin completely, leaving a clear zone in the agar around the growing cells or colonies. complete hemolysis betahemolysis Wikipedia:hemolysis_(microbiology) Beta hemolysis (β-hemolysis), is caused by the secretion of beta-hemolysin by a pathogenic bacterium. Beta-hemolysin is a protein which catalyzes the hydrolysis of sphingomyelin, releasing phosphoryl choline and resulting in lysis fo the red blood cell. The result are colonies on blood agar that are surrounded by a yellow, transparent zone. gamma-hemolysis assay Carrine Blank A hemolysis assay for the process of gamma-hemolysis, where a protein (beta-haemolysin) secreted by a microorganism results in the hydrolysis of sphingomyelin in erythrocytes (red blood cells) and thus causing the cells to lyse. Gamma-hemolysis does not break dow the red blood cells, and thus no clearing is seen. Wikipedia:hemolysis_(microbiology) Gamma hemolysis, caused by the secretion of gamma-hemolysin, results in the lysis of leucocytes (white blood cells). Red blood cells are unaffected, and therefore do not show hemolytic activity on blood agar plates. erythrocyte hemagglutination assay HA haemagglutinated Carrine Blank Wikipedia:hemagglutination Hemagglutination, or haemagglutination, is a specific form of agglutination that involves red blood cells (RBCs). It has two common uses in the laboratory: blood typing and the quantification of virus dilutions. hemagglutinated A microbiological assay for binding to blood cells, where a microorganism causes red blood cells to clump (agglutinate). haemagglutinates hemagglutinates haemagglutination cytadsorption cytadherence Heterotypic cell-cell adhesion where a prokaryotic cell is able to adhere to another cell. Carrine Blank Staley vitamin solution From: Staley JT. 1981. The genera Prosthecomicrobium and Ancalomicrobium. In: Starr MP, Stolp H, Truper HG, Balows A,Schlegel HG, (eds). The prokaryotes. Berlin: Springer-Verlag. Pg 68. Vitamin Solution B12 0.1 mg Biotin 2 mg Calcium pantothenate 5 mg Folic acid 2 mg nicotinamide 5 mg pyridoxine HCl 10 mg riboflavin 5 mg thiamine HCL 5 mg Add distilled water up to 1 L and store in 4˚C in a dark container. A solution of B vitamins including cobalamin (B12), biotin (B7), pantothenic acid (B5), folic acid (B9), nicotinamide (B3), pyridoxine (B6), riboflavin (B2), and thiamine (B1). Used to support the growth of microorganisms. Carrine Blank trace elements solution Carrine Blank A solution of trace elements (or trace metals), sometimes also having an organic chelator of metal ions (such as EDTA or nitrilotriacetic acid). Used to support the growth of microorganisms. trace metals 44 metals "44" metals 44 A trace elements solution containing disodium EDTA, zinc sulfate, ferrous sulfate, manganese sulfate, copper sulfate, cobalt nitrate, and sodium tetraborate. Carrine Blank From http://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=149 Metals ''44'': EDTA·2Na 250.0 mg ZnSO4·7H2O 1095.0 mg FeSO4·7H2O 500.0 mg MnSO4·xH2O 154.0 mg CuSO4·5H2O 39.2 mg Co(NO3)2·6H2O 24.8 mg Na2B4O7·10H2O 17.7 mg Distilled water 1.0 L soil extract Carrine Blank A water extract of soil. Soil extract is often prepared by sieving air dried soil through a coarse sieve and autoclaving distilled water. Used to support the growth of microorganisms. chopped lean ground beef Chopped and ground meat (muscle tissue) containing a low fat content from beef (Bos taurus). Used to culture microorganisms. lean ground beef Carrine Blank microbiological gelling agent A polymeric organic compound or mix of organic compounds which are liquid under autoclave conditions but solidify under the growth conditions used to cultivate a microorganism. Used to make solid microbiological culture media. Carrine Blank vitamin solution Defined organic solution comprised of a mixture of vitamins (used as enzyme co-factors) added in small amounts to microbiological culture medium. Used to support the growth of microorganisms. Carrine Blank homogenized chicken egg Microbiological medium ingredient, derived from chicken egg, where the chicken egg has been homogenized (mixed). Carrine Blank fish peptone An enzymatic hydrolysis of protein derived from fish (of unspecified origin), used for the cultivation of microorganisms. Carrine Blank microbiological medium ingredient, derived from enzymatic hydrolysis of mammalian protein Carrine Blank Protein hydrolysates, also called peptones, are the result of the enzymatic hydrolysis of animal protein (mammal meat, muscle tissue). heart pancreatic digest Carrine Blank A pancreatic digest of heart tissue derived from animal (mammalian) tissue, used for the culturing of microorganisms. microbiological medium ingredient, derived from enzymatic hydrolysis of milk protein Protein hydrolysates, also called peptones, are the result of the enzymatic hydrolysis of milk proteins (such as casein or lactalbumin). Carrine Blank peptone from casein pancreatic digest of gelatin pancreatic digest of gelatin peptone gelatine peptone A pancreatic digest of gelatin, extracted from collagen derived from bone, cartilage, and connective tissue (of unspecified origin). Carrine Blank gelatin peptone gelatine peptone, pancreatic Gelysate(TM) peptone microbiological culture medium, promotes growth of autotrophs A microbiological culture medium that lacks organic carbon compounds and therefore promotes the growth of autotrophic microorganisms. Carrine Blank mineral salts media Mineral salts growth medium microbiological culture medium, promotes growth of heterotrophs A microbiological culture medium that contains organic carbon compounds and therefore promotes the growth of heterotrophic microorganisms. Carrine Blank Carboxymethyl cellulose agar A mineral-salts, solid microbiological culture medium with yeast extract, ammonium phosphate, and carboxymethyl cellulose. Used for the cultivation of microorganisms capable of metabolizing cellulose. CMC-containing plates From: BugwoodWiki:CMCA (Carboxymethylcellulose agar) Medium contains: Part 1: Distilled water, 1 L NH4H2PO4, 1 g KCl, 0.2 g MgSO4*7H2O, 1 g Yeast extract, 1 g Part 2: Carboxymethyl cellulose, 26 g Agar, 3g Mix ingredients from Part 1 and add ingredients from Part 2 while stirring Autoclave 20 minutes. cellulose gum agar Carrine Blank carboxymethylcellulose plates CMC plates CMCA agar plate medium Carrine Blank A solid microbiological culture medium that has been solidified by agar and poured into a flat surface in a Petri dish. hypersaline microbiological culture medium A microbiological culture medium that has a salinity of greater than 5% salt. Carrine Blank marine microbiological culture medium Carrine Blank A microbiological culture medium that has a salinity of 3-5 % salts. brackish microbiological culture medium Carrine Blank A microbiological culture medium that has a salinity of greater than 0.05 % salts and less than 3% salts. prokaryotic physiological quality Carrine Blank A prokaryotic quality relating the optimal physiological preference (pressure, salinity, temperature) of a particular strain of prokaryotes. When these preferred conditions are met, the organism will exhibit cell division. barophilic Wikipedia:piezophile Barophilic microorganisms grow at high pressures. They are typically isolated from deep subsurface or deep sea habitats. Pressure optimum quality, where growth rates at elevated pressures are higher than growth rates at atmospheric pressure. Generally a characteristic of prokaryotic microorganisms that grow in the deep oceans. piezophile Carrine Blank piezophilic barophile requires magnesium for growth Magnesium required Magnesium is required Mg2+ required Magnesium (II) ions are required to be present in order for growth of a particular microorganism to occur. Carrine Blank microbiological diagnostic assay An assay used to identify or characterize a microorganism. Is used to determine the biological process, quality, cellular component, molecular function, or a chemical entity that is associated with the particular microorganism. Carrine Blank urease assay urease URE urea hydrolysis urease test An enzymatic assay which tests for the presence of urease in a microorganism. Urease hydrolyzes urea to produce CO2 and ammonia, which increases the pH of the media: (NH2)2CO + H2O -> CO2 + 2 NH3 The urease test uses phenol red as a pH indicator. An increase in alkalinity results in a color from yellow to pink/red. A positive result is pink/orange/red; a negative test result is yellow. The test employs either Christensen's Urea Agar or Stuart's Urea Broth. http://www.microbelibrary.org/library/laboratory-test/3223-urease-test-protocol Carrine Blank barotolerant Wikipedia:piezophlie Barotolerant microorganisms are able to grow or survive at high pressures, but can also grow and survive at atmospheric pressures. Pressure optimum quality, where some growth occurs at elevated pressures, but growth is fastest at atmospheric pressures. facultative barophile piezotolerant Carrine Blank facultatively barophilic obligately barophilic Barophilic, and can only grow at elevated pressures. These organisms require elevated pressure to grow and survive. Wikipedia:piezophile Carrine Blank obligate piezophile aerophilicity The metabolic quality of a microorganism with respect to its relationship to oxygen. Carrine Blank aerobicity fermentation metabolic process Carrine Blank fermentation fermenter ferment ferments fermentatively fermentative Wikipedia:fermentation Fermentation is a metabolic process of microorganisms which converts a carbohydrate carbon and energy substrate into acids, gases, and/or alcohols. fermenting fermented fermentable A prokaryotic metabolic process where the carbon and energy sources are organic compounds. Is an anaerobic process which does not require an electron transport system. respiratory metabolic process A prokaryotic metabolic process which involves the oxidation and reduction of chemical compounds and involves an electron transport chain. Carrine Blank aerobic Carrine Blank Wikipedia:aerobic organism An aerobic organism or aerobe is an organism that can survive and grow in an oxygenated environment. Facultative anaerobes grow and survive in an oxygenated environment and so do aerotolerant anaerobes. aerobic A prokaryotic metabolic quality where the organism grows in the presence of oxygen. aerobically anaerobic anoxic Carrine Blank anaerobic Wikipedia:anaerobic_organism An anaerobic organism or anaerobe is any organism that does not require oxygen for growth. It may react negatively or even die if oxygen is present, which means that it can perform its bodily functions better in the absence of oxygen. An anaerobic organism may be unicellular or multicellular (like metazoa or more complex organisms like deep sea worms). A few parasites like Trichinella spiralis (pork worm) respire anaerobically in nurse cells (infected cell playing host to the juvenile parasite). Some largely unicellular anaerobic microbes are protozoans, but most of the anaerobic microbes are bacteria or Archaea. For practical purposes there are three categories: obligate anaerobes, which are harmed by the presence of oxygen aerotolerant organisms, which cannot use oxygen for growth, but tolerate the presence of it facultative anaerobes, which can grow without oxygen but can utilize oxygen if it is present anaerobically A prokaryotic metabolic quality where the organism grows in the absence of oxygen. chemotrophy Wikipedia:Chemotroph Chemotrophs are organisms that obtain energy by the oxidation of electron donors in their environments. These molecules can be organic (chemoorganotrophs) or inorganic (chemolithotrophs). The chemotroph designation is in contrast to phototrophs, which utilize solar energy. Chemotrophs can be either autotrophic or heterotrophic. A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source. Carrine Blank mixotrophy Wikipedia:Mixotroph A mixotroph is an organism that can use a mix of different sources of energy and carbon. Possible alternations are between photo- and chemotrophy, between litho- and organotrophy, between auto- and heterotrophy or a combination of it. Mixotrophs can be either eukaryotic or prokaryotic. They can take advantage of different environmental conditions. If a trophic mode is obligate, then it is always necessary for sustaining growth and maintenance; if facultative, it can be used as a supplemental source. Some organisms have incomplete Calvin cycles, so they are incapable of fixing carbon dioxide and must use organic carbon sources. A prokaryotic respiratory metabolic process where phototrophy and heterotrophy co-occur at the same instance in time. Carrine Blank phototrophy A prokaryotic respiratory metabolic process where light is the energy source. Carrine Blank Wikipedia:Phototroph Phototrophs are the organisms that carry out photon capture to acquire energy. They use the energy from light to carry out various cellular metabolic processes. Most of the well-recognized phototrophs are autotrophs, also known as photoautotrophs, and can fix carbon. photolithotrophy A prokaryotic respiratory metabolic process where light is the energy source, and the electron donor is an inorganic compound. Wikipedia:Lithotroph Photolithotrophs obtain energy from light and therefore use inorganic electron donors only to fuel biosynthetic reactions (e.g., carbon dioxide fixation in lithoautotrophs). These bacteria are photosynthetic; photolithotrophic bacteria are found in the purple bacteria (e. g., Chromatiaceae), green bacteria (Chlorobiaceae and Chloroflexi) and Cyanobacteria. Purple and green bacteria oxidize sulfide, sulfur, sulfite, iron or hydrogen. Cyanobacteria extract reducing equivalents from water, i.e., they oxidise water to oxygen. The electrons obtained from the electron donors are not used for ATP production (as long as there is light); they are used in biosynthetic reactions. Some photolithotrophs shift over to chemolithotrophic metabolism in the dark. Carrine Blank photolithoautotrophy Carrine Blank Wikipedia:Microbial metabolism Photolithoautotrophs obtain energy from light and carbon from the fixation of carbon dioxide, using reducing equivalents from inorganic compounds. Examples: Cyanobacteria (water (H2O) as reducing equivalent donor), Chlorobiaceae, Chromatiaceae (hydrogen sulfide (H2S) as reducing equivalent donor), Chloroflexus (hydrogen (H2) as reducing equivalent donor). A prokaryotic respiratory metabolic process where light is the energy source, the electron donor is an inorganic compound, and the carbon source is carbon dioxide photofermentation A prokaryotic metabolic process that involves fermentation in the light. Is carried out by photosynthetic prokaryotic microorganisms. Carrine Blank Wikipedia: Photofermentation Photofermentation is the fermentative conversion of organic substrate to biohydrogen manifested by a diverse group of photosynthetic bacteria by a series of biochemical reactions involving three steps similar to anaerobic conversion. Photofermentation differs from dark fermentation because it only proceeds in the presence of light. For example photo-fermentation with Rhodobacter sphaeroides SH2C (or many other purple non-sulfur bacteria[1]) can be employed to convert small molecular fatty acids into hydrogen[2] and other products. photofermentative aerotolerant Wikipedia:anaerobic_organism An anaerobic organism or anaerobe is any organism that does not require oxygen for growth. For practical purposes there are three categories: obligate anaerobes, which are harmed by the presence of oxygen aerotolerant organisms, which cannot use oxygen for growth, but tolerate the presence of it facultative anaerobes, which can grow without oxygen but can utilize oxygen if it is present Carrine Blank A prokaryotic metabolic quality where the organism grows in the absence of oxygen. Can grow or live in the presence of oxygen. facultatively anaerobic facultative anaerobe facultatively anaerobic facultatively aerobic Wikipedia:facultative_anaerobic_organism A facultative anaerobic organism is an organism, usually a bacterium, that makes ATP by aerobic respiration if oxygen is present but is also capable of switching to fermentation. In contrast, obligate anaerobes die in the presence of oxygen. Carrine Blank facultative anaerobe A prokaryotic metabolic quality where the organism grows in the presence of oxygen or in the absence of oxygen. facultative aerobe obligately anaerobic obligate anaerobic Wikipedia:obligate_anaerobe Obligate anaerobes are microorganisms that live and grow in the absence of molecular oxygen; some of these are killed by oxygen. strictly anoxic strictly anaerobic obligately anaerobic A prokaryotic metabolic quality where the organism grows in the absence of oxygen. Cannot grow or live in the presence of oxygen. obligatory anaerobic Carrine Blank chemolithotrophy Carrine Blank A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source, and the electron donor is an inorganic compound. Wikipedia:Lithotroph A chemolithotroph (named after the process of chemolithotropy) is a type of extremophile that is able to use inorganic reduced compounds as a source of energy. This process is accomplished through oxidation and ATP synthesis. Most chemolithotrophs are able to fix carbon dioxide (CO2) through the Calvin Cycle, a metabolic pathway in which carbon enters as CO2 and leaves as glucose. This group of organisms includes sulfur oxidizers, nitrifying bacteria, iron oxidizers, and hydrogen oxidizers. The term chemolithotropy refers to a cell’s acquisition of energy from the oxidation of inorganic compounds, also known as electron donors, in the absence of light. Chemolithotropy is a form of metabolism, and the extreme conditions under which it can take place leads to its classification as a branch of the extremophiles. This process only occurs in prokaryotes, and was first characterized by the Russian microbiologist Sergej Winogradsky. chemoorganotrophy A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source, and the electron donor is an organic compound. Wikipedia:Primary nutritional groups Chemoorganotrophs are organisms which oxidize the chemical bonds in organic compounds as their energy source. Chemoorganotrophs also attain the carbon molecules that they need for cellular function from these organic compounds. The organic compounds that they oxidize include sugars (i.e. glucose), fats and proteins).[2] Carrine Blank photolithoheterotrophy Carrine Blank A prokaryotic respiratory metabolic process where light is the energy source, the electron donor is an inorganic compound, and the carbon source is an organic compound. photoorganotrophy A prokaryotic respiratory metabolic process where light is the energy source, and the electron donor is an organic compound. photoheterotroph Wikipedia:Photoheterotroph Photoheterotrophs are heterotrophic organisms that use light for energy, but cannot use carbon dioxide as their sole carbon source. Consequently, they use organic compounds from the environment to satisfy their carbon requirements; these compounds include carbohydrates, fatty acids, and alcohols. Examples of photoheterotrophic organisms include purple non-sulfur bacteria, green non-sulfur bacteria, and heliobacteria. Carrine Blank photoorganoautotrophy Carrine Blank A prokaryotic respiratory metabolic process where light is the energy source, the electron donor is an organic compound, and the carbon source is an carbon dioxide. photoorganoheterotrophy Wikipedia:Microbial metabolism Photoorganoheterotrophs obtain energy from light, carbon and reducing equivalents for biosynthetic reactions from organic compounds. Some species are strictly heterotrophic, many others can also fix carbon dioxide and are mixotrophic. Examples: Rhodobacter, Rhodopseudomonas, Rhodospirillum, Rhodomicrobium, Rhodocyclus, Heliobacterium, Chloroflexus (alternatively to photolithoautotrophy with hydrogen). Carrine Blank A prokaryotic respiratory metabolic process where light is the energy source, the electron donor is an organic compound, and the carbon source is an organic compound. chemolithoautotrophy Wikipedia:Microbial metabolism Chemolithoautotrophs obtain energy from the oxidation of inorganic compounds and carbon from the fixation of carbon dioxide. Examples: Nitrifying bacteria, Sulfur-oxidizing bacteria, Iron-oxidizing bacteria, Knallgas-bacteria. Carrine Blank A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an inorganic compound, and the carbon source is carbon dioxide. chemolithoheterotrophy Wikipedia:Microbial metabolism Chemolithoheterotrophs obtain energy from the oxidation of inorganic compounds, but cannot fix carbon dioxide (CO2). Examples: some Thiobacillus, some Beggiatoa, some Nitrobacter spp., Wolinella (with H2 as reducing equivalent donor), some Knallgas-bacteria, some sulfate-reducing bacteria. Carrine Blank A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an inorganic compound, and the carbon source is an organic compound. chemoorganoautotrophy A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an organic compound, and the carbon source is carbon dioxide. Carrine Blank chemoorganoheterotrophy A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an organic compound, and the carbon source is an organic compound. Carrine Blank Wikipedia:Primary nutritional groups A chemoorganoheterotrophic organism is one that requires organic substrates to get its carbon for growth and development, and that produces its energy from oxido-reduction of an organic compound. This group of organisms may be further subdivided according to what kind of organic substrate and compound they use. Decomposers are examples of Chemoorganoheterotrophs which obtain carbon and electron reactions from dead organic matter. Herbivores and carnivores are examples of organisms that obtain carbon and electron reactions from living organic matter. heterotroph microaerophilic A prokaryotic metabolic quality where the organism grows in the presence of oxygen, where the oxygen concentration is less than atmospheric levels. Wikipedia:microaerophile A microaerophile is a microorganism that requires oxygen to survive, but requires environments containing lower levels of oxygen than are present in the atmosphere (~20% concentration). Many microphiles are also capnophiles, as they require an elevated concentration of carbon dioxide. In the laboratory they can be easily cultivated in a candle jar. A candle jar is a container into which a lit candle is introduced before sealing the container's airtight lid. The candle's flame burns until extinguished by oxygen deprivation, which creates a carbon dioxide-rich, oxygen-poor atmosphere in the jar. microaerobically Microaerophilically microaerobic microaerophilic Carrine Blank microoxic obligately aerobic A prokaryotic metabolic quality where the organism grows in the presence of oxygen. Is incapable of anaerobic growth (growth in the absence of oxygen). Wikipedia:obligate_aerobe An obligate aerobe is an aerobic organism that requires oxygen to grow. Through cellular respiration, these organisms use oxygen to oxidize substances, like sugars or fats, in order to obtain energy. During respiration, they use oxygen as the terminal electron acceptor. They have the advantage of yielding more energy than obligate anaerobes, but face high levels of oxidative stress. obligatory aerobic obligately aerobic Carrine Blank strictly aerobic microbiological medium ingredient, derived from extracts of plants Undefined mixtures of complex organic compounds deriving from the aqueous extraction (an extraction using water, such as hot water or steam) of macroscopic plants. Used in the cultivation of microorganisms. Carrine Blank defined organic chemical mixture Organic compounds or mixtures of organic compounds added to culture media to support growth or metabolism of a microorganism. Because the exact composition of the mixture is known, it is referred to as "defined". Carrine Blank clarified rumen fluid Undefined organic chemical mixture, comprised of ruminal fluid derived from the rumen of a cow, which has been clarified (through the processes of heating/autoclaving and then centrifugation). Used to cultivate microorganisms. rumen fluid Tureen fluid Carrine Blank sludge fluid From Wikipedia:Sludge: Sludge is a semi-solid slurry and can be produced as sewage sludge from wastewater treatment processes or as a settled suspension obtained from conventional drinking water treatment and numerous other industrial processes. Carrine Blank Fluid derived from the liquid component of municipal sludge, used for the cultivation of microorganisms. Yeastolate Carrine Blank The water-soluble portion of autolyzed yeast (Saccharomyces cereviseiae), used to support the growth of microorganisms. Yeastolate is a branch name for a yeast extract sold by BD. TC Proteose Yeastolate From BD Bionutrients Technical Manual (3rd edition revised): TC Yeastolate products are animal-free and water-soluble portions of autolyzed yeast or Saccharomyces cerevisiae. TC Yeastolate is a mixture of peptides, amino acids, carbohydrates, simple and complex as well as vitamins. Ash content is 11.7% NaCl content is 0.6% Bacto(TM) TC Yeastolate multicellular prokaryote Carrine Blank multicellular A prokaryotic cellularity where cells are physically attached, or connected, to one another after cell division occurs. Septation may or may not occur. The connections between the cells may be loose and transient, or may be long-lasting and permanent. chocolatized defibrinated blood Carrine Blank From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Chocolatized Defibrinated Blood is defibrinated blood that has been heated to release additional growth factors that would otherwise remain unavailable within intact red blood cells. Heat stable hemin (X-factor) and heat labile nicotinamide adenine dinucleotide (V-factor) are the growth factors readily available in chocolatized media. Blood medium ingredient comprised of defibrinated blood that has been heated. Used in the cultivation of microorganisms. inspissated serum Serum medium ingredient comprised of blood serum from a mammal that has been inspissated (dehydrated, i.e. has a decreased water composition). Used to support the growth of microorganisms which need inspissated blood serum to grow. From Wikipedia:Inspissation: Inspissation is the process of thickening by dehydration. Carrine Blank Spissated serum prokaryotic differentiated cell Carrine Blank Prokaryotic cells which have a morphological shape and a role that is different from vegetative (undifferentiated cells). prokaryotic differentiated cell, specialized for nitrogen fixation Prokaryotic differentiated cell that has a role in the fixation of gaseous dinitrogen (N2) into ammonia and thus enhance survival in nitrogen-deficient environments. Carrine Blank prokaryotic differentiated cell, specialized for asexual reproduction Prokaryotic differentiated cell that has a role in asexual reproduction. Carrine Blank prokaryotic differentiated cell, specialized for dormancy Prokaryotic differentiated cell which contributes to enhanced dormancy and thus survival during times unsuitable for growth. Shioi marine medium An organic-rich, marine, liquid microbiological culture medium containing yeast extract, polypeptone, casamino acids, and glycerol in an artificial sea water base. Used for the growth Erythrobacter. Shioi Y. 1986. Growth characteristics and substrate specificity of aerobic photosynthetic bacterium, Erythrobacter sp. Och-114. Plant Cell Physiol 27:567-572. Shioi (1986) used a medium which contains in 1 liter of distilled water: 20 g NaCl, 5.0 g MgCl2x6H2O, 2.0 g Na2SO4, 0.5 g KCl, 0.5 g CaCl2x2H2O, 0.2 g NaHCO3, 0.1 g ferric citrate, 2.0 g yeast extract (Difco), 1.0 g polypeptone, 1.0 g Casamino acids, and 1.0 mL glycerol. The pH is not in any available references online or in Bergey's Manual. However is likely slightly alkaline due to the buffering of sodium bicarbonate. Shioi's marine medium SMM medium Carrine Blank SMM media Shiba T & Imhoff JF. 2005. Genus XVIII. Roseobacter in Bergey's Manual of Systematic Bacteriology, 2nd Ed. Vol 2, Part C, p. 213. SM medium Carrine Blank From: http://cshprotocols.cshlp.org/content/2008/12/pdb.rec11558.full?text_only=true SM Broth Composition: Reagent Quantity (for 1 L) Final concentration Glucose 10 g 56 mM KH2PO4 1.9 g 14 mM K2HPO4 0.6 g 3.4 mM NH4Cl 1 g 20 mM Proteose peptone 2 ( Difco 212120) 10 g 1% Yeast extract (Oxoid LP0021) 1 g 0.1% Dissolve in 1 L of H2O. Adjust the pH to 6.0–6.4 with KOH. SM broth A liquid microbiological culture medium containing gelatin, magnesium sulfate, and sodium chloride. Used for the storage and dilution of Escherichia coli bacteriophage lambda. CMRL-1066 medium with glutamine From: https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/c0422dat.pdf CMRL-1066 With L-Glutamine, Without Sodium Bicarbonate Components g/L L-Alanine 0.025 L-Arginine 0.05787 L-Aspartic Acid 0.03 L-Cysteine•HCl• H2O 0.26 L-Cystine 0.02 L-Glutamic Acid 0.075 L-Glutamine 0.1 Glycine 0.05 L-Histidine HCl• H2O 0.02 Trans-4-Hydroxy-L-Proline 0.01 L-Isoleucine 0.02 L-Leucine 0.06 L-Lysine•HCl 0.07 L-Methionine 0.015 L-Phenylalanine 0.025 L-Proline 0.04 L-Serine 0.025 L-Threonine 0.03 L-Tryptophan 0.01 L-Tyrosine 0.04 L-Valine 0.025 L-Ascorbic Acid 0.05 PABA 0.00005 D-Biotin 0.00001 Choline Chloride 0.0005 Coenzyme A•Na 0.0025 Cocarboxylase 0.001 2'-Deoxyadenosine 0.01 2'-Deoxyguanosine 0.01 2'-Deoxycytidine•HCl 0.0116 Flavin Aadenine Dinucleotide•2Na 0.000106 Folic Acid 0.00001 myo-Inositol 0.00005 5-Methyldeoxycytidine 0.0001 beta-NAD 0.007 beta-NADP•Na 0.001 Niacinamide 0.000025 Nicotinic Acid 0.000025 D-Pantothenic Acid [hemicalcium] 0.00001 Pyridoxal•HCl 0.000025 Pyridoxine•HCl 0.000025 Riboflavin 0.00001 Thiamine•HCl 0.00001 Thymidine 0.01 Uridine-5-Triphosphate•Na 0.001 Calcium Chloride [Anhydrous] 0.2 Magnesium Sulfate [Anhydrous] 0.09769 Potassium Chloride 0.4 Sodium Acetate [Anhydrous] 0.05 Sodium Chloride 6.8 Sodium Phosphate Monobasic [Anhydrous] 0.122 D-Glucose 1.0 Phenol Red•Na 0.02124 Glutathione 0.01 D-Glucuronic Acid•Na 0.00388 Cholesterol 0.0002 Tween 80 0.005 Sodium bicarbonate is added during the preparation step and the pH is adjusted to 7.0 ± 0.3. A defined liquid microbiological culture medium containing glucose, a pH indicator, a mixture of amino acids, vitamins, co-factors, and organic micronutrients. Used for tissue cell culture. skim milk medium From: Skim Milk Medium (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Skim Milk Medium may be used for the cultivation and differentiation of microorganisms based on the coagulation and proteolysis of casein. Principles of the Procedure Skim Milk is a source of lactose and casein and other nutrients required for the growth of lactobacilli.4 Clostridial species can be differentiated based on their ability to enzymatically degrade proteins to peptones (peptonization) or coagulate milk.5 It may be used to detect the stormy fermentation produced by Clostridium perfringens. Formula Difco(TM) Skim Milk Approximate Formula* Per Liter Skim Milk Powder 100.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.3 ± 0.2 Carrine Blank An organic-rich, liquid microbiological culture medium containing skim milk powder. Used to cultivate microorganisms that can metabolize casein. skim milk agar From: Skim Milk Medium (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Skim Milk Medium may be used for the cultivation and differentiation of microorganisms based on the coagulation and proteolysis of casein. Principles of the Procedure Skim Milk is a source of lactose and casein and other nutrients required for the growth of lactobacilli.4 Clostridial species can be differentiated based on their ability to enzymatically degrade proteins to peptones (peptonization) or coagulate milk.5 It may be used to detect the stormy fermentation produced by Clostridium perfringens. Formula Difco(TM) Skim Milk Approximate Formula* Per Liter Skim Milk Powder 100.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.3 ± 0.2 An organic-ric, solid microbiological culture medium containing skim milk powder. Used to cultivate microorganisms that can metabolize casein. Skim milk media containing agar. Columbia sheep blood agar Carrine Blank Sheep blood agar Columbia blood agar CSB agar CBA Columba-based sheep blood agar An organic-rich, solid microbiological culture medium, containing peptones, starch, and sheep blood. Used for the growth of heterotrophic microorganisms that require blood. Heart infusion agar supplemented with sheep blood CSB From: Columbia Agar with 5% Sheep Blood (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Columbia Agar Base, without or with the addition of 5% (or 10%) sheep blood, is a highly nutritious, general-purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical materials. Difco™ Columbia Blood Agar Base Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 10.0 g Proteose Peptone No. 3................................................ 5.0 g Yeast Extract................................................................ 5.0 g Beef Heart, Infusion from 500 g................................... 3.0 g Corn Starch.................................................................. 1.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g Directions for Preparation from Dehydrated Product 1. Suspend the powder in 1 L of purified water: Difco™ Columbia Blood Agar Base – 44 g; BBL™ Columbia Agar Base – 42.5 g; Difco™ Columbia Blood Agar Base EH – 39 g. Mix thoroughly. 2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3. Autoclave at 121°C for 15 minutes. 4. For preparation of blood agar, cool the base to 45-50°C and add 5% sterile, defibrinated blood. Mix well. 5. Test samples of the finished product for performance using stable, typical control cultures. Columbia Agar Base An organic-rich, solid microbiological culture medium, containing peptones and starch. Used for the growth of heterotrophic microorganisms. Carrine Blank From: Columbia Agar Base (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Columbia Agar Base, without or with the addition of 5% (or 10%) sheep blood, is a highly nutritious, general-purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical materials. Principles of the Procedure Columbia Agar Base supplemented with sheep, rabbit or horse blood derives its superior growth-supporting properties from the combination of peptones prepared from pancreatic digest of casein, meat peptic digest and heart pancreatic digest. Yeast extract and corn starch are also included in the formulation and serve as energy sources with yeast extract being a supplier of the B-complex vitamins. Sodium chloride maintains osmotic balance in the medium. It should be noted that Columbia Sheep Blood Agar has a relatively high carbohydrate content and, therefore, beta-hemolytic streptococci may produce a greenish hemolytic reaction that may be mistaken for alpha hemolysis. Fildes enrichment is prepared by the action of the enzyme pepsin on defibrinated sheep blood. Bacitracin is a polypeptide antibiotic that is active mainly against gram-positive bacteria. BBL™ Columbia Agar Base Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 10.0 g Meat Peptic Digest....................................................... 5.0 g Yeast Extract................................................................ 5.0 g Heart Pancreatic Digest................................................ 3.0 g Corn Starch.................................................................. 1.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 13.5 g pH 7.3 ± 0.2 seawater agar Humphry DR, George A, Black GW & Cummings SP. 2001. Flavobacteirum frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminariolytic bacterium from Antarctica. IJSEM 51:1235-1243. From: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium246.pdf DSMZ Medium 246 246. SEA WATER AGAR Beef extract 10.0 g Peptone 10.0 g Agar 20.0 g Tap water 250.0 ml Sea water* 750.0 ml Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Directly after autoclaving, add warm (55°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature. *Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. Artificial sea water: NaCl 28.13 g KCl 0.77 g CaCl2 x 2 H2O 1.60 g MgCl2 x 6 H2O 4.80 g NaHCO3 0.11 g MgSO4 x 7 H2O 3.50 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved An organic-rich, marine, solid microbiological culture medium containing beef extract and peptone, in a diluted artificial sea water base. Used for the growth of marine Flavobacterium. Carrine Blank ZoBell medium, NaCl-free From: Yi H, Cho J-C, & Chun J. 2012. Flavivirga jejuensis gen. nov., sp. nov., and Flavivirga amylovorans sp. nov., new members of the family Flavobacteriaceae isolated from seawater, and emended descriptions of the genera Psychroserpens and Lacinutrix. IJSEM 62:1061-1068. Growth in synthetic ZoBell broth was tested at 5-50˚C (at 5˚C intervals) and in the presence of 0, 0.5, 1, 2, 3, 4, 5, 7, 10, 12, 15 and 20% (w/v) NaCl or sea salts (Sigma). Sea salts-free Zobell's medium An organic-rich, mineral-salts, liquid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Made without sodium chloride. Used to test the salinity preference of marine heterotrophic microorganisms. Carrine Blank rabbit laked blood agar An organic-rich, solid microbiological culture medium containing peptones, yeast extract, sodium chloride, glucose, sodium sulfite, hemin, vitamin K, and laked rabbit blood. Used for the growth of heterotrophic microorganisms that require blood. Carrine Blank From: Atlas RM, Handbook of Microbiological Media, 3rd edition, pg. 1472 Composition per liter: Agar 15.0 g Pancreatic digest of casein 10.0 g Peptic digest of animal tissue 10.0 g NaCl 5.0 g Yeast extract 2.0 g Glucose 1.0 g NaHSO3 0.1 g Rabbit blood, laked 50.0 mL Hemin solution 1.0 mL Vitamin K1 solution 1.0 mL pH 7.0 +/- 0.2 at 25˚C Hemin solution: Composition per 100.0 mL: Hemin 0.5 g NaOH (1N solution) 10.0 mL Preparation of Hemin Solution: Add hemin to 10.0 mL of NaOH solution. Mix thoroughly. Vitamin K1 solution: Composition per 20.0 mL: Vitamin K1 (phytomenadione) 0.2 g Ethanol (95% solution) 20.0 mL Preparation of Vitamin K1 Solution: Add vitamin K1 to 20.0 mL of ethanol. Mix thoroughly. Preparation of Medium: Add components, except vitamin K1 solution and laked rabbit blood, to distilled/deionized water and bring volume to 849.0 mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121˚C. Cool to 45-55˚C. Aseptically add 1.0 mL of sterile vitamin K1 solution and 50.0 mL of sterile laked rabbit blood. Laked blood is prepared by freezing whole blood overnight and thawing to room temperature. Mix thoroughly. Pour into steril Petri dishes or distribute into sterile tubes. Use: For the cultivation and enhancement of pigment production of a variety of anaerobic bacteria. R2A medium An organic-rich, liquid microbiological culture medium containing yeast extract, proteose peptone, casamino acids, glucose, starch, and pyruvate. Used for the growth of heterotrophic bacteria from potable (fresh) water. Reasoner's 2A From: Wikipedia: R2a agar R2A agar (Reasoner´s 2A agar) is a culture medium developed to study bacteria which normally inhabit potable water. These bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium. Since its development in 1979, it has been found to allow the culturing of many other bacteria that will not readily grow on fuller, complex organic media. Typical Composition (g/l) Proteose peptone, 0.5 Casamino acids, 0.5 Yeast extract, 0.5 Dextrose, 0.5 Soluble starch, 0.5 Dipotassium phosphate, 0.3 Magnesium sulfate 7H2O, 0.05 Sodium pyruvate, 0.3 Agar, 15 Final pH 7 ± 0.2 R2A broth Carrine Blank R3A medium Carrine Blank From: Reasoner DJ & Geldreich EE. 1985. A new medium for the enumeration and subculture of bacteria from potable water. AEM 49(1):1-7. Composiotn of R3A Experimental Media (From Table 1): Yeast extract, 1.0 g/L Difco Proteose Peptone No. 3, 1.0 g/L Casamino Acids, 1.0 g/L Glucose, 1.0 g/L Soluble starch, 1.0 g/L Sodium pyruvate, 0.5 g/L K2HPO4, 0.6 g/L MgSO4x7H2O, 0.1 g/L Agar, 15.0 g/L Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C and 15 lb/in2. An organic-rich, liquid microbiological culture medium containing yeast extract, proteose peptone, casamino acids, glucose, starch, and pyruvate. Concentration of organic and inorganic constituents are twice that in R2A medium. Used for the growth of heterotrophic bacteria from potable (fresh) water. R2A agar An organic-rich, solid microbiological culture medium containing yeast extract, proteose peptone, casamino acids, glucose, starch, and pyruvate. Used for the growth of heterotrophic bacteria from potable (fresh) water. Carrine Blank R2A plates From: Wikipedia: R2a agar R2A agar (Reasoner´s 2A agar) is a culture medium developed to study bacteria which normally inhabit potable water. These bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium. Since its development in 1979, it has been found to allow the culturing of many other bacteria that will not readily grow on fuller, complex organic media. Typical Composition (g/l) Proteose peptone, 0.5 Casamino acids, 0.5 Yeast extract, 0.5 Dextrose, 0.5 Soluble starch, 0.5 Dipotassium phosphate, 0.3 Magnesium sulfate 7H2O, 0.05 Sodium pyruvate, 0.3 Agar, 15 Final pH 7 ± 0.2 YPD broth GYEA An organic-rich, liquid microbiological culture medium containing yeast extract, peptone, and dextrose (D-glucose). Used for the cultivation of yeasts. PYG medium peptone yeast glucose broth peptone/yeast-extract/glucose broth From: Yeast Extract-Peptone-Dextrose Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use YPD Agar and YPD Broth are used for maintaining and propagating yeasts in molecular microbiology procedures. Principles of the Procedure YPD Agar and YPD Broth contain peptone as a source of carbon, nitrogen, vitamins and minerals. Yeast extract supplies B-complex vitamins which stimulate bacterial growth. Dextrose is the carbohydrate source. YPD Agar contains agar as the solidifying agent. Formulae Difco™ YPD Agar Approximate Formula* Per Liter Yeast Extract.............................................................. 10.0 g Peptone..................................................................... 20.0 g Dextrose.................................................................... 20.0 g Agar.......................................................................... 15.0 g Difco™ YPD Broth Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 6.5 ± 0.2 Carrine Blank peptone-yeast extract-glucose Glucose-yeast extract agar pH is 6.5 PYG broth YPD agar PYG plates Carrine Blank An organic-rich, solid microbiological culture medium containing yeast extract, peptone, and dextrose (D-glucose). Used for the cultivation of yeasts. pH is 6.5 PYG agar From: Yeast Extract-Peptone-Dextrose Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use YPD Agar and YPD Broth are used for maintaining and propagating yeasts in molecular microbiology procedures. Principles of the Procedure YPD Agar and YPD Broth contain peptone as a source of carbon, nitrogen, vitamins and minerals. Yeast extract supplies B-complex vitamins which stimulate bacterial growth. Dextrose is the carbohydrate source. YPD Agar contains agar as the solidifying agent. Formulae Difco™ YPD Agar Approximate Formula* Per Liter Yeast Extract.............................................................. 10.0 g Peptone..................................................................... 20.0 g Dextrose.................................................................... 20.0 g Agar.......................................................................... 15.0 g Difco™ YPD Broth Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 6.5 ± 0.2 peptone-yeast extract-glucose agar YPD-Tween medium Carrine Blank An organic-rich, liquid microbiological culture medium containing yeast extract, peptone, and glucose. Supplemented with Tween 80 (0.02%, aka polysorbate 80). Used for the cultivation of Bacteroides spp. PYG-Tween Peptone-yeast extract-tween basal medium From: Cato EP, Holdeman LV, & Moore WEC. 1979. Proposal of neotype strains for seven non-saccharolytic Bacteroides species. IJSB 29(4):427-434. Growth was stimulated by the addition of ca. 0.02% Tween 80 to the media. PYE medium Peptone-yeast extract broth PY Carrine Blank PYE broth From: Kampfer P, Busse H-J, Longaric I, Rossello-Mora R, Galatis H, & Lodders N. 2012. Pseudarcicella hirudinis gen. nov., sp. nov., isolated from the skin of the medical leech Hirudo medicinalis. IJSEM 62:2247-2251. 0.3% (w/v) peptone from casein 0.3% (w/v) yeast extract pH 7.2 at 28˚C An organic-rich, liquid microbiological culture medium containing casitone and yeast extract. Used for the growth of Pseudarcicella sp. peptone water medium peptone-water medium Carrine Blank From: Peptone Water (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Peptone Water is used for cultivating nonfastidious organisms, for studying carbohydrate fermentation patterns and for performing the indole test. Principles of the Procedure Peptone Water contains peptone as a source of carbon, nitrogen, vitamins and minerals. Sodium chloride maintains the osmotic balance of the medium. Formula Difco™ Peptone Water Approximate Formula* Per Liter Peptone..................................................................... 10.0 g Sodium Chloride.......................................................... 5.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.2 ± 0.2 An organic-rich, liquid microbiological culture medium containing peptone and sodium chloride. peptone water plate count agar PCA An organic-rich, solid microbiological culture medium containing pancreatic digest of casein, yeast extract, and dextrose. Used for the enumeration of microorganisms that grow in dairy products. Standard methods agar Tryptone Glucose Yeast Extract Agar PCA agar Tryptone glucose yeast agar Carrine Blank From: Plate Count Agar/Standard Methods Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Plate Count Agar and Standard Methods Agar (Plate Count Agar; Tryptone Glucose Yeast Agar) are used for obtaining microbial plate counts from milk and dairy products, foods, water and other materials of sanitary importance. Principles of the Procedure Enzymatic digest of casein provides the amino acids and other complex nitrogenous substances necessary to support bacterial growth. Yeast extract primarily supplies the B-complex vitamins, and dextrose is an energy source. TTC is reduced to the insoluble formazan inside the bacterial cell producing red-colored colonies. Formula Difco™ Plate Count Agar or BBL™ Standard Methods Agar Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 5.0 g Yeast Extract................................................................ 2.5 g Dextrose...................................................................... 1.0 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2 TGY agar pca medium Pseudomonas isolation agar PIA An organic-rich, solid microbiological culture medium containing peptone, potassium sulfate, magnesium chloride, and Irgasan (aka triclosan; a broad spectrum antibiotic). Is a selective medium used for the isoaltion and growth of Pseudomonas. From: Pseudomonas Isolation Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Pseudomonas Isolation Agar is used with added glycerol in isolating Pseudomonas and differentiating Pseudomonas aeruginosa from other pseudomonads based on pigment formation. Principles of the Procedure Peptone provides the carbon and nitrogen necessary for bacterial growth. Magnesium chloride and potassium sulfate promote production of pyocyanin. Irgasan, an antimicrobial agent, selectively inhibits gram-positive and gram-negative bacteria other than Pseudomonas spp. Agar is the solidifying agent. Glycerol serves as an energy source and also helps to promote pyocyanin production. Formula Difco™ Pseudomonas Isolation Agar Approximate Formula* Per Liter Peptone..................................................................... 20.0 g Magnesium Chloride.................................................... 1.4 g Potassium Sulfate....................................................... 10.0 g Irgasan™..................................................................... 25.0 mg Agar.......................................................................... 13.6 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2 Carrine Blank OF basal agar O/F medium Leifson's O/F medium Hugh and Leifson's OF basal medium oxidation fermentation medium Leifson's O-F medium Hugh and Leifson's Carrine Blank OF basal medium An organic-rich, liquid microbiological culture medium containing peptone, glucose, sodium chloride, and a pH indicator (bromthymol blue). Used to test for the ability of a microorganism to metabolize specific carbohydrates, which are added to the basal medium. From: http://www.microbelibrary.org/component/resource/laboratory-test/3151-oxidative-fermentative-test-protocol RECIPE Hugh and Leifson’s OF basal medium (6, 8) Peptone (tryptone) 2.0 g Sodium chloride 5.0 g Glucose (or other carbohydrate)a 10.0 g Bromthymol blue 0.03 g Agar 3.0 g Dipotassium phosphate 0.30 g Bring to 1 liter with distilled water. The pH should be adjusted to 7.1 prior to autoclaving (7). After the medium is autoclaved at 121°C for 15 minutes, a filter sterilized solution of 10% solution of carbohydrate (6, 8) is aseptically added to the medium to a final concentration of 1%. The sterile medium containing the carbohydrate is aliquoted aseptically into sterile test tubes and cooled unslanted as stabs (5). Some procedures call for the addition of 10 g/liter of carbohydrate to the medium prior to sterilization. The medium is then dissolved by heating to a boil on a hot plate or by steaming for 20 minutes prior to aliquoting into test tubes. The tubed medium is then steamed for 20 minutes in place of autoclaving to prevent breaking down of the carbohydrate (7). OF basal medium is commercially available in a premixed form from biological supply companies. The carbohydrate source is not included and must be added as stated above. OF agar with MCS From: Marine Cations Supplement 1558 (From: Farmer, J. J., III & Hickman-Brenner, F. W. (2006). The genera Vibrio and Photobacterium. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 6, pp. 508–563. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer., pages 532 and 535). This medium is useful for increasing the salt content of bacteriological media to enhance the growth of marine bacteria. It contains Na+, K+, Mg++, and Ca++ at 10 times the in-use concentration. It is our formulation which was modified from the “electrolyte supplement” of Furniss et al. (1978), and it is added in the ratio of one volume of supplement to nine volumes of medium. Sodium chloride (NaCl) 150 g Potassium chloride (KCl) 3.7 g Magnesium chloride (MgCl2 · 6H2O) 51 g Calcium chloride (CaCl2 · 2H20) 7.4 g Water 912 ml Dissolve the ingredients in the order listed. All should dissolve readily, and a crystal-clear, colorless solution should result. The volume of the solution will expand to 1 liter after the salts dissolve. Dispense and autoclave at 121°C for 15 minutes. It should remain crystal clear after autoclaving, but a slight amount of fine precipitate may form and settle to the bottom of the container. To use this supplement in other media: aseptically add one volume of marine cations, supplement 1558 to nine volumes of the sterile medium, and mix thoroughly. An organic-rich, solid microbiological culture medium containing peptone, glucose, sodium chloride, and a pH indicator (bromthymol blue). Supplemented with MCS (marine cations supplement), which includes sodium chloride, potassium chloride, magnesium chloride, and calcium chloride. Used to test for the ability of a marine microorganism to metabolize specific carbohydrates, which are added to the basal medium. Carrine Blank O/F medium (with MCS) Lucena T, Pascual J, Giordano A, Gambacorta A, Garay E, Arahal DR, Macian MC, & Pujalte MJ. 2010. Euzebyella saccharophila gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae. IJSEM 60:2871-2876. MCS = marine cations supplement nutrient agar 1.5% From: Nutrient Agar 1.5 % (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Nutrient Agar 1.5% is used for cultivating a variety of microorganisms and with the addition of blood or other enrichment can be used for cultivating fastidious microorganisms. Principles of the Procedure Beef extract and peptone provide the nitrogen, vitamins, amino acids and carbon sources in Nutrient Agar 1.5%. Sodium chloride maintains the osmotic balance so that red blood cells will not rupture when blood is added as supplement.1 Agar is the solidifying agent. Formula Difco™ Nutrient Agar 1.5% Approximate Formula* Per Liter Beef Extract.................................................................. 3.0 g Peptone....................................................................... 5.0 g Sodium Chloride.......................................................... 8.0 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.2 Carrine Blank An organic-rich, solid microbiological culture medium containing beef extract, peptone and sodium chloride (8g/L). Used for the cultivation of general heterotrophic microorganisms. MRS agar A slightly-acidic, organic-rich, solid microbiological culture medium containing peptones, egg extract, yeast extract, glucose, Tween 80, and minerals salts. Used for the cultivation of Lactobacilli. Carrine Blank de Man-Rogosa-Sharpe medium From: Wikipedia:MRS_agar Often abbreviated to MRS, this type of bacterial growth medium is so-named by its inventors: de Man, Rogosa and Sharpe. Developed in 1960, this medium was designed to favour the luxuriant growth of Lactobacilli for lab study. It contains sodium acetate, which suppresses the growth of many competing bacteria (although some other Lactobacillales, like Leuconostoc and Pediococcus, may grow). This medium has a clear brown colour.[1] MRS agar typically contains(w/v):[2] 1.0 % peptone 0.8 % egg extract 0.4 % yeast extract 2.0 % glucose 0.5 % sodium acetate trihydrate 0.1 % polysorbate 80 (also known as Tween 80) 0.2 % dipotassium hydrogen phosphate 0.2 % triammonium citrate 0.02 % magnesium sulfate heptahydrate 0.005 % manganese sulfate tetrahydrate 1.0 % agar pH adjusted to 6.2 at 25°C MM1 medium Carrine Blank A dilute organic carbon-containing, solid microbiological culture medium with glucose, yeast extract, and divalent mineral-salts. Used for the cultivation of Mucilagibacter sp. The pH of the medium is not specified, and the medium itself is unbuffered. The optimal pH of growth for the organism in the study, however, was pH 6.0-6.5. From: Pankratov TA, Tindall BJ, Liesack W, & Dedysh SN. 2007. Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. IJSEM 57:2349-2354. All tests were carried out using medium MM1 containing (per liter distilled water) 0.5 g glucose, 0.04 g MgSO4.7H2O, 0.02 g CaCl2.2H2O and 0.1 g yeast extract. filtered tomato juice An undefined organic chemical mixture derived from the juice of a tomato (the fruit of Solanum lycopersicum), formed through the process of maceration (homogenization) followed by filtration. Carrine Blank Mueller-Hinton agar MHA Carrine Blank From: Mueller Hinton Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Each lot of Mueller Hinton Agar and Mueller Hinton II Agar has been tested according to, and meets the acceptance limits of, the current M6 protocol published by the CLSI. Mueller Hinton Agar is recommended for antimicrobial disc diffusion susceptibility testing of common, rapidly growing bacteria by the Bauer-Kirby method, as standardized by the Clinical and Laboratory Standards Institute (CLSI). Summary and Explanation Mueller Hinton Agar was originally developed for the cultivation of pathogenic Neisseria.6 However, these organisms are now commonly isolated on selective media. Principles of the Procedure Acid hydrolysate (digest) of casein and beef extract supply amino acids and other nitrogenous substances, minerals, vitamins, carbon and other nutrients to support the growth of microorganisms. Starch acts as a protective colloid against toxic substances that may be present in the medium. Hydrolysis of the starch during autoclaving provides a small amount of dextrose, which is a source of energy. Agar is the solidifying agent. The Bauer-Kirby procedure is based on the diffusion through an agar gel of antimicrobial substances which are impregnated on paper discs.16 In contrast to earlier methods which used discs of high and low antimicrobial concentrations and which used the presence or absence of inhibition zones for their interpretation, this method employs discs with a single concentration of antimicrobial agent and zone diameters are correlated with minimal inhibitory concentrations (MIC).1,2,4,7,16 In the test procedure, a standardized suspension of the organism is swabbed over the entire surface of the medium. Paper discs impregnated with specified amounts of antibiotic or other antimicrobial agents are then placed on the surface of the medium, the plate is incubated and zones of inhibition around each disc are measured. The determination as to whether the organism is susceptible, intermediate or resistant to an agent is made by comparing zone sizes obtained to those in the CLSI Document M100(M2).4 Various factors have been identified as influencing disc diffusion susceptibility tests. These include the medium, excess surface moisture on the medium, agar depth, disc potency, inoculum concentration, pH and β-lactamase production by test organisms. 7,13,16 Formulae Difco™ Mueller Hinton Agar Approximate Formula* Per Liter Beef Extract Powder..................................................... 2.0 g Acid Digest of Casein................................................. 17.5 g Starch.......................................................................... 1.5 g Agar.......................................................................... 17.0 g pH 7.3 ± 0.1 BBL™ Mueller Hinton II Agar Approximate Formula* Per Liter Beef Extract.................................................................. 2.0 g Acid Hydrolysate of Casein......................................... 17.5 g Starch.......................................................................... 1.5 g Agar.......................................................................... 17.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.1 An organic-rich, solid microbiological culture medium containing beef extract, casein hydrolysate, and starch. Used for the cultivation of Neisseria. MacConkey agar MAC Macconkey agar Maccongkey agar Carrine Blank An organic-rich, selective solid microbiological culture medium that contains peptones, lactose, bile salts, crystal violet, and a pH indicator (neutral red). The bile salts inhibit swarming in Proteus spp., and the crystal violet inhibits growth of some Gram-positive bacteria. Lactose fermentation lowers the pH, resulting in colonies that are red or pink in color, and causes the bile salts to precipitate. Non-lactose fermenters that can deaminate amino acids in the peptone release ammonia, which causes the pH to increase (and thus the colonies appear white). MacConkey medium Mac-Conkey agar From MacConkey Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use MacConkey agars are slightly selective and differential plating media mainly used for the detection and isolation of gram-negative organisms from clinical,1-3 dairy,4 food,5-7 water,8 pharmaceutical, 9-11 cosmetic,6,7 and other industrial sources. MacConkey Agar is used for isolating and differentiating lactose-fermenting from lactose-nonfermenting gram-negative enteric bacilli. Principles of the Procedure Peptones are sources of nitrogen and other nutrients. Yeast extract is a source of trace elements, vitamins, amino acids and carbon. Lactose is a fermentable carbohydrate. When lactose is fermented, a local pH drop around the colony causes a color change in the pH indicator (neutral red) and bile precipitation. Bile salts, bile salts no. 3, oxgall and crystal violet are selective agents that inhibit growth of gram-positive organisms. Sodium chloride maintains osmotic balance in the medium. Magnesium sulfate is a source of divalent cations. Agar is the solidifying agent. Formulae Difco™ MacConkey Agar Approximate Formula* Per Liter Pancreatic Digest of Gelatin....................................... 17.0 g Peptones (meat and casein).......................................... 3.0 g Lactose...................................................................... 10.0 g Bile Salts No. 3............................................................. 1.5 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 13.5 g Neutral Red.................................................................. 0.03 g Crystal Violet............................................................... 1.0 mg pH 7.1 ± 0.2 Difco™ MacConkey Agar Base Consists of the same ingredients without the lactose. BBL™ MacConkey Agar Approximate Formula* Per Liter Pancreatic Digest of Gelatin....................................... 17.0 g Peptones (meat and casein).......................................... 3.0 g Lactose...................................................................... 10.0 g Bile Salts...................................................................... 1.5 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 13.5 g Neutral Red.................................................................. 0.03 g Crystal Violet............................................................... 1.0 mg pH 7.1 ± 0.2 litmus lactose agar An organic-rich, solid microbiological culture medium that contains meat peptone, lactose and litmus (a pH indicator). Lactose fermenting bacteria will turn the pH indicator red as the pH decreases; colonies will be surrounded by a red zone. Colonies that turn blue are the result of the deamination of amino acids, resulting when the pH increases. Vandamme P1, Segers P, Ryll M, Hommez J, Vancanneyt M, Coopman R, De Baere R, Van de Peer Y, Kersters K, De Wachter R, Hinz KH. 1998. Pelistega europaea gen. nov., sp. nov., a bacterium associated with respiratory disease in pigeons: taxonomic structure and phylogenetic allocation. IJSB 48(2):431-440. Carrine Blank From Fluka Analytical: 62628 LL Agar (Litmus Lactose Agar according to Drigalski, Drigalski Litmus Lactose Agar) Selective medium for the differentiation of several bacteria based on lactose fermentation, in the inspection of water, milk, meat and other foodstuffs. Composition: Ingredients Grams/Litre Meat peptone 7.0 Sodium chloride 5.0 Lactose 15.0 Litmus 1.2 Agar 13.0 Final pH 7.4+/-0.2 at 37°C Store prepared media below 8°C, protected from direct light. Store dehydrated powder, in a dry place, in tightly-sealed containers at 2-25°C. Directions: Dissolve 41 g in 1 litre distilled water. Autoclave at 121°C for 15 minutes. Principle and Interpretation: LL Agar was formulated by Drigalski for the differentiation of Iactose fermenting and Iactose nonfermenting bacteria. It is importante for the detection of enteric pathogens in the inspection of water, milk, meat and other foodstuffs. Meat peptone provide nitrogenous nutrients to the organisms. Lactose is fermented by lactose fermenting bacteria with acid production. Litmus is the pH indicator which turns red at acidic pH. Colonies of the Iactose fermenting bacteria are surrounded by a red zone which distinguishes them from colonies of other organisms that either do not change the surrounding medium or make it blue due to production of ammonia. Inoculate culture from primary fermentation tubes (e.g. with Lactose Broth; Fluka 70142) showing gas production either by surface inoculation (using inoculation loop) or by pour plate method of serially diluted culture. Cultural characteristics after 24-72 hours at 35ºC . lecithovitellin agar Carrine Blank Lecithovitellin Solution (Methods in Microbiology, v. 6, pt. 1, Chapter 1, Routine Biochemical Tests, pg 18): ...."which is made by adding 1 egg yolk to 225 mL of saline buffered with 0.1 M borate buffer at pH 7.2-7.4 with 0.005 M CaCl2; 10 g of a filtration aid such as Hyflo Supercel (Johns Manville Co., London) is added and the mixture shaken for 1 h before filtering twice through Whatman No. 1 papers and finally Seitz-filtering with negative pressure." An organic-rich, solid microbiological culture medium based on nutrient agar, where lecithovitellin (a substance derived from the chemical treatment of chicken egg yolks) is added. Recipe (from Cowan and Steel's Manual for the Identification of Medial Bacteria, 1993, ed. Barrow & Feltham, 3rd ed, pg. 205): Lecithovitellin Agar: Lecithovitellin solution (or egg yolk emulsion) 100 mL Nutrient agar 900 mL Melt the nutrient agar and cool to about 55˚C. Add the lecithovitellin solution aseptically, mix and pour plates. LV agar LBM medium An organic-rich, solid microbiological culture medium containing tryptone, yeast extract, in a base of synthetic sea water. Used to cultivate Tenacibaculum sp. Carrine Blank From: Suzuki M, Nakagawa Y, Harayama S, & Yamamoto S. 2001. Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. IJSEM 51:1639-1652. 2.0 g tryptone and 1.0 g yeast extract in 1000 mL Jamarin S synthetic sea water (Jamarin Laboratory) at pH 7.2 LB medium LB broth Luria broth From: LB Broth, Miller (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use LB Agar, Miller and LB Broth, Miller (Luria-Bertani) are used for maintaining and propagating Escherichia coli in molecular microbiology procedures. Principles of the Procedure Peptone provides nitrogen and carbon. Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Agar is the solidifying agent in LB Agar, Miller. Formulae Difco™ LB Agar, Miller Approximate Formula* Per Liter Tryptone.................................................................... 10.0 g Yeast Extract................................................................ 5.0 g Sodium Chloride........................................................ 10.0 g Agar.......................................................................... 15.0 g Difco™ LB Broth, Miller Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2 An organic-rich, liquid microbiological culture medium containing tryptone, yeast extract, and sodium chloride. Used to cultivate Escherichia coli. Carrine Blank LB agar From: LB Agar, Miller (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use LB Agar, Miller and LB Broth, Miller (Luria-Bertani) are used for maintaining and propagating Escherichia coli in molecular microbiology procedures. Principles of the Procedure Peptone provides nitrogen and carbon. Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Agar is the solidifying agent in LB Agar, Miller. Formulae Difco™ LB Agar, Miller Approximate Formula* Per Liter Tryptone.................................................................... 10.0 g Yeast Extract................................................................ 5.0 g Sodium Chloride........................................................ 10.0 g Agar.......................................................................... 15.0 g Difco™ LB Broth, Miller Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2 Carrine Blank An organic-rich, solid microbiological culture medium containing tryptone, yeast extract, and sodium chloride. Used to cultivate Escherichia coli. LBA laked blood agar From: http://medical-dictionary.thefreedictionary.com/laked+blood laked blood [lākt] Etymology: Fr, laque, a deep red color blood that is clear, red, and homogenous because of hemolysis of the red blood cells, as may occur in poisoning and severe extensive burns. A solid microbiological culture medium (a blood agar), made with laked blood (where the red blood cells have been treated so that they have undergone haemolysis). Haemolysed blood agar Laked BAP Laked blood agar plates Carrine Blank ISP 2 agar Carrine Blank From: Shirling, E. B. & Gottlieb, D. (1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340. Medium 2: Yeast-extract - malt extract agar (Pridham et al., 1956-57) Bacto-Yeast Extract (Difco) 4.0g Bact-Malt Extract (Difco) 10.0 g Bacto-Dextrose (Difco) 4.0 g Distilled water 1.0 liter Adjust to pH 7.3, then add -- Bacto agar 20.0 g Liquefy agar by steaming at 100˚C for 15-20 minutes. Dispense appropriate amount for a slanting into at least 6 tubes for each culture. Sterilize by autoclaving; cool tubes as slants. Use the agar slants for preparation of stock cultures. Also sterilize medium 2 in flasks for pouring the sterilized medium into Petri dishes. ISP medium 2 Yeast extract-malt extract agar An organic-rich, solid microbiological culture medium, containing yeast extract, malt extract, and dextrose. Used to culture Streptomyces sp. brain heart infusion agar Carrine Blank An organic-rich, solid microbiological culture medium containing water infusions of calf brains and beef hearts, proteose peptone, and dextrose (i.e. D-glucose). From: Brain Heart Infusion Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Brain Heart Infusion (BHI) Agar is a general-purpose medium suitable for the cultivation of a wide variety of organism types, including bacteria, yeasts and molds. With the addition of 5% or 10% sheep blood, it is used for the isolation and cultivation of a wide variety of fungal species, including systemic fungi(1) from clinical and nonclinical sources. Principles of the Procedure BHI Agar derives its nutrients from the brain heart infusion, peptone and dextrose components. The peptones and infusion are sources of organic nitrogen, carbon, sulfur, vitamins and trace substances. Dextrose is a carbohydrate source that microorganisms utilize by fermentative action. The medium is buffered through the use of disodium phosphate. When defibrinated sheep blood is added to the basal medium, it provides essential growth factors for the more fastidious fungal organisms. Formulae Difco™ Brain Heart Infusion Agar Approximate Formula* Per Liter Calf Brains, Infusion from 200 g................................... 7.7 g Beef Heart, Infusion from 250 g................................... 9.8 g Proteose Peptone....................................................... 10.0 g Dextrose...................................................................... 2.0 g Sodium Chloride.......................................................... 5.0 g Disodium Phosphate.................................................... 2.5 g Agar.......................................................................... 15.0 g pH 7.4 ± 0.2 BBL™ Brain Heart Infusion Agar Approximate Formula* Per Liter Brain Heart, Infusion from (solids)................................. 8.0 g Peptic Digest of Animal Tissue...................................... 5.0 g Pancreatic Digest of Casein........................................ 16.0 g Dextrose...................................................................... 2.0 g Sodium Chloride.......................................................... 5.0 g Disodium Phosphate.................................................... 2.5 g Agar.......................................................................... 13.5 g pH 7.4 ± 0.2 BBL™ Brain Heart Infusion Agar, Modified Approximate Formula* Per Liter Brain Heart, Infusion from (solids)................................. 3.5 g Peptic Digest of Animal Tissue.................................... 15.0 g Pancreatic Digest of Casein........................................ 10.0 g Dextrose...................................................................... 2.0 g Sodium Chloride.......................................................... 5.0 g Disodium Phosphate.................................................... 2.5 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.4 ± 0.2 heart infusion agar An organic-rich, solid microbiological culture medium that contains (beef) heart infusion and tryptose. Used to grow a wide range of heterotrophic microorganisms. From: Heart Infusion Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Heart Infusion Agar is a general-purpose medium used in the cultivation of a wide range of microorganisms from a variety of clinical and nonclinical specimens. Principles of the Procedure Heart Infusion Agar derives its nutrients from heart muscle infusion and peptone, which supply nitrogenous and carbonaceous compounds, sulfur, vitamins and trace ingredients. Sodium chloride maintains osmotic equilibrium. Agar is the solidifying agent. The addition of 5% sheep blood provides additional growth factors and is used to determine hemolytic reactions. Formula Difco™ Heart Infusion Agar Approximate Formula* Per Liter Beef Heart, Infusion from 500 g................................. 10.0 g Tryptose..................................................................... 10.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.4 ± 0.2 Carrine Blank heart infusion medium Carrine Blank An organic-rich, liquid microbiological culture medium that contains (beef) heart infusion and tryptose. Used to grow a wide range of heterotrophic microorganisms. From: Heart Infusion Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Bacto™ Heart Infusion Broth is used for cultivating fastidious microorganisms. Principles of the Procedure Heart Infusion Agar derives its nutrients from heart muscle infusion and peptone, which supply nitrogenous and carbonaceous compounds, sulfur, vitamins and trace ingredients. Sodium chloride maintains osmotic equilibrium. Agar is the solidifying agent. The addition of 5% sheep blood provides additional growth factors and is used to determine hemolytic reactions. Formula Bacto™ Heart Infusion Broth Approximate Formula* Per Liter Beef Heart, Infusion from 500 g................................. 10.0 g Tryptose..................................................................... 10.0 g Sodium Chloride.......................................................... 5.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.4 ± 0.2 brain heart infusion medium brain heart infusion broth From: Brain Heart Infusion Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Brain Heart Infusion (BHI) Agar is a general-purpose medium suitable for the cultivation of a wide variety of organism types, including bacteria, yeasts and molds. With the addition of 5% or 10% sheep blood, it is used for the isolation and cultivation of a wide variety of fungal species, including systemic fungi(1) from clinical and nonclinical sources. Principles of the Procedure BHI Agar derives its nutrients from the brain heart infusion, peptone and dextrose components. The peptones and infusion are sources of organic nitrogen, carbon, sulfur, vitamins and trace substances. Dextrose is a carbohydrate source that microorganisms utilize by fermentative action. The medium is buffered through the use of disodium phosphate. When defibrinated sheep blood is added to the basal medium, it provides essential growth factors for the more fastidious fungal organisms. Formulae Difco™ Brain Heart Infusion Agar Approximate Formula* Per Liter Calf Brains, Infusion from 200 g................................... 7.7 g Beef Heart, Infusion from 250 g................................... 9.8 g Proteose Peptone....................................................... 10.0 g Dextrose...................................................................... 2.0 g Sodium Chloride.......................................................... 5.0 g Disodium Phosphate.................................................... 2.5 g Agar.......................................................................... 15.0 g pH 7.4 ± 0.2 BBL™ Brain Heart Infusion Agar Approximate Formula* Per Liter Brain Heart, Infusion from (solids)................................. 8.0 g Peptic Digest of Animal Tissue...................................... 5.0 g Pancreatic Digest of Casein........................................ 16.0 g Dextrose...................................................................... 2.0 g Sodium Chloride.......................................................... 5.0 g Disodium Phosphate.................................................... 2.5 g Agar.......................................................................... 13.5 g pH 7.4 ± 0.2 BBL™ Brain Heart Infusion Agar, Modified Approximate Formula* Per Liter Brain Heart, Infusion from (solids)................................. 3.5 g Peptic Digest of Animal Tissue.................................... 15.0 g Pancreatic Digest of Casein........................................ 10.0 g Dextrose...................................................................... 2.0 g Sodium Chloride.......................................................... 5.0 g Disodium Phosphate.................................................... 2.5 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.4 ± 0.2 Carrine Blank An organic-rich, liquid microbiological culture medium containing water infusions of calf brains and beef hearts, proteose peptone, and dextrose (i.e. D-glucose). Czapek-Dox medium Carrine Blank Czapek-Dox broth From: Czapek-Dox Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Czapek-Dox Broth and Czapek Solution Agar are used for cultivating fungi and bacteria capable of using inorganic nitrogen. Principles of the Procedure Saccharose is the sole carbon source, and sodium nitrate is the sole nitrogen source in Czapek-Dox Broth and Czapek Solution Agar. Dipotassium phosphate is the buffering agent, and potassium chloride contains essential ions. Magnesium sulfate and ferrous sulfate are sources of cations. Agar is the solidifying agent in Czapek Solution Agar. Formulae Difco™ Czapek-Dox Broth Approximate Formula* Per Liter Saccharose................................................................. 30.0 g Sodium Nitrate............................................................. 3.0 g Dipotassium Phosphate................................................ 1.0 g Magnesium Sulfate...................................................... 0.5 g Potassium Chloride...................................................... 0.5 g Ferrous Sulfate............................................................. 0.01 g pH 7.3 ± 0.2 An organic-rich, liquid microbiological culture medium used for the cultivation of fungi and bacteria that can use saccharose (i.e. sucrose) as a sole carbon source and nitrate as a sole nitrogen source. inorganic chemical mixture Inorganic compounds or mixtures of inorganic compounds added to culture media to support growth or metabolism of a microorganism. Carrine Blank Czapek-Dox agar From: Czapek-Dox Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Czapek-Dox Broth and Czapek Solution Agar are used for cultivating fungi and bacteria capable of using inorganic nitrogen. Principles of the Procedure Saccharose is the sole carbon source, and sodium nitrate is the sole nitrogen source in Czapek-Dox Broth and Czapek Solution Agar. Dipotassium phosphate is the buffering agent, and potassium chloride contains essential ions. Magnesium sulfate and ferrous sulfate are sources of cations. Agar is the solidifying agent in Czapek Solution Agar. Formulae Difco™ Czapek Solution Agar Approximate Formula* Per Liter Saccharose................................................................. 30.0 g Sodium Nitrate............................................................. 2.0 g Dipotassium Phosphate................................................ 1.0 g Magnesium Sulfate...................................................... 0.5 g Potassium Chloride...................................................... 0.5 g Ferrous Sulfate............................................................. 0.01 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.2 Carrine Blank An organic-rich, solid microbiological culture medium used for the cultivation of fungi and bacteria that can use saccharose (i.e. sucrose) as a sole carbon source and nitrate as a sole nitrogen source. chopped meat carbohydrate medium An organic-rich, liquid microbiological culture medium containing pancreatic digest of casein, yeast extract, phosphate buffer, glucose, maltose, cellobiose, starch, hemin and Vitamin K1. Used for the growth of anaerobes such as Clostridia. Chopped meat carbohydrate culture CMC From: Item AS-823, http://www.anaerobesystems.com/Home/pras-tubed-media/chopped-meat-carbohydrate-broth Formula Lean Ground Beef, 500.0 g Sodium Hydroxide (1N), 25.0 ml Pancreatic Digest of Casein, 30.0 g Yeast Extract, 5.0 g Potassium Phosphate, Dibasic, 5.0 g L-Cysteine, 0.5 g Hemin (0.1% Soln), 5.0 ml Vitamin K1 (1% Soln), 0.1 ml Dextrose, 4.0 g Maltose, 1.0 g Cellobiose, 1.0 g Starch, 1.0 g Distilled Water, 1000.0 ml Final pH 7.1 +/- 0.4 at 25 degrees C. Final volume 7.0 mL +/- 0.7. Carrine Blank Chopped meat carbohydrate broth tryptose medium An organic-rich, liquid microbiological culture medium containing typtose, dextrose (D-glucose), and sodium chloride. Used for the cultivation of Brucella. Carrine Blank From: Tryptose Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Tryptose Broth is used for cultivating Brucella and other fastidious microorganisms. Principles of the Procedure Tryptose peptone is a source of nitrogen and carbon. Dextrose is a source of carbohydrate. Sodium chloride maintains osmotic balance. Agar is the solidifying agent in Tryptose Agar. Formulae Difco™ Tryptose Agar Approximate Formula* Per Liter Tryptose..................................................................... 20.0 g Dextrose...................................................................... 1.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g Difco™ Tryptose Broth Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.2 ± 0.2 tryptose agar Carrine Blank From: Tryptose Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Tryptose Agar is used for cultivating a wide variety of fastidious microorganisms, particularly for isolating Brucella according to Huddleson and Castañeda. Principles of the Procedure Tryptose peptone is a source of nitrogen and carbon. Dextrose is a source of carbohydrate. Sodium chloride maintains osmotic balance. Agar is the solidifying agent in Tryptose Agar. Formulae Difco™ Tryptose Agar Approximate Formula* Per Liter Tryptose..................................................................... 20.0 g Dextrose...................................................................... 1.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.2 ± 0.2 An organic-rich, solid microbiological culture medium containing typtose, dextrose (D-glucose), sodium chloride and agar. Used for the cultivation of Brucella. basic fuchsin agar Carrine Blank From Morgan WJB. 1961. The use of the thionin blue sensitivity test in the examination of Brucella. J Gen Microbiol 25:135-139. "Serum glucose agar was used for the proposation of cultures and as basal medium for the addition of dyes. The composition of this medium has been described (Morgan, 1960). Basic fuchsin (supplied by Pharmaceutical Laboratories National Aniline Division, Allied Chemical and Dye Corporation, New York) was added to the melted and cooled medium just before pouring plate to give a concentration of 1/25,000. Thionin (Allied Chemical and Dye Corp.) was used at a concentration of 1/50,000. Initially, three concentrations of thionin blue (British Drug Houses Ltd., London) were used, namely, 1/500,000, 1/1,000,000, 1/2,000,000. Later however only the 1/500,000 concentration was used for routine use. Stock solutions of the dyes were made and steam sterilized. All new batches were checked for activity against known strains. All plates were incubated overnight at 37˚ before use and no plate was used which had been stored for longer than one week in the refrigerator." Huddleson's basic fuchsin medium A tyrosine agar (or liver infusion agar, or glucose agar) supplemented with the pH indicator Basic Fuchsin (aka rosanilin, Basic Fuchsine, Rosaniline Hydrochloride, Magenta). If acid is produced, the basic fuchsin will turn deep red/pink. From Huddleson IF. 1961. Emergence during growth of Brucella strains on dye-agar media of cells that show changes in sulfur metabolism. Bull Wld Hlth Org 24:91-102. "Initial differences in the growth of strains on agar containing thionin and basic fuchsin were determined by lightly inoculating the surface of tryptose agar in Petri plates containing separately 1 mg thionin/100 mL (basic aqueous solution 100 mg/100 mL) and 1 mg basic fuchsin/100 mL (basic ethanol solution 100 mg/100 mL). " pg. 94. thionin agar Tyrosine agar (or liver infusion agar, or glucose agar) with thionin blue stain (akas Thionin, Thionine, Thionine acetate, or Lauth's Violet) added. From: Huddleson IF. 1961. Emergence during growth of Brucella strains on dye-agar media of cells that show changes in sulfur metabolism. Bull Wld Hlth Org 24:91-102. "Initial differences in the growth of strains on agar containing thionin and basic fuchsin were determined by lightly inoculating the surface of tryptose agar in Petri plates containing separately 1 mg thionin/100 mL (basic aqueous solution 100 mg/100 mL) and 1 mg basic fuchsin/100 mL (basic ethanol solution 100 mg/100 mL). " pg. 8. Huddleson's thionin agar Carrine Blank Huddleson's thionin medium HCY medium Carrine Blank From: Zaichikova MV, Berestovskaya YY, Kuznetsov BB, & Vasileva LV. 2013. Spirosoma xylofaga sp. nov., an oligotrophic pleomorphic bacterium from a myco-bacterial community of freshwater ecosystems. Microbiology 82(4):459-465. Pure culture was maintained in liquid HCY medium containing Hutner's basal salt solution (20 mL/L), a vitamin mixture [5], xylose (0.5 g/L) as a substrate, and yeast extract (0.01 g/L). [Ref 5]: Zaichikova, M.V., Berestovskaya, Akimov, V.N., Kizilova, A.K., and Vasileva, L.V., Xantobacter xylophilus sp. nov., a member of the xylotrophic myco-bacterial community of low mineral oligotrophic waters, Microbiology, 2010, vol. 79, no. 1, pp. 83-88. From http://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=631 Hunter's basal salts: Nitrilotriacetic acid 10.0 g MgSO4·7H2O 14.45 g CaCl2·2H2O 4.42 g (NH4)6Mo7O24·4H2O 9.25 mg FeSO4·7H2O 99.0 mg Nicotinic acid 50.0 mg Thiamine·HCl 25.0 mg Biotin 0.5 mg Metals ''44'' (see Medium No. 149) 50.0 ml Distilled water 950.0 ml Dissolve and neutralized nitrilotriacetic acid in 500.0 ml distilled water with the addition of KOH. Add the remaining chemicals in order listed. Adjust pH to 6.8 and bring volume to 1.0 L with distilled water. From http://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=149 Metals ''44'': EDTA·2Na 250.0 mg ZnSO4·7H2O 1095.0 mg FeSO4·7H2O 500.0 mg MnSO4·xH2O 154.0 mg CuSO4·5H2O 39.2 mg Co(NO3)2·6H2O 24.8 mg Na2B4O7·10H2O 17.7 mg Distilled water 1.0 L A mineral-salts, liquid microbiological culture medium containing magnesium sulfate and nitrilotriacetic acid. Used to support the growth of Spirosoma and Xanthobacter. HCY agar From http://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=631 Hunter's basal salts: Nitrilotriacetic acid 10.0 g MgSO4·7H2O 14.45 g CaCl2·2H2O 4.42 g (NH4)6Mo7O24·4H2O 9.25 mg FeSO4·7H2O 99.0 mg Nicotinic acid 50.0 mg Thiamine·HCl 25.0 mg Biotin 0.5 mg Metals ''44'' (see Medium No. 149) 50.0 ml Distilled water 950.0 ml Dissolve and neutralized nitrilotriacetic acid in 500.0 ml distilled water with the addition of KOH. Add the remaining chemicals in order listed. Adjust pH to 6.8 and bring volume to 1.0 L with distilled water. From http://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=149 Metals ''44'': EDTA·2Na 250.0 mg ZnSO4·7H2O 1095.0 mg FeSO4·7H2O 500.0 mg MnSO4·xH2O 154.0 mg CuSO4·5H2O 39.2 mg Co(NO3)2·6H2O 24.8 mg Na2B4O7·10H2O 17.7 mg Distilled water 1.0 L Carrine Blank From: Zaichikova MV, Berestovskaya YY, Kuznetsov BB, & Vasileva LV. 2013. Spirosoma xylofaga sp. nov., an oligotrophic pleomorphic bacterium from a myco-bacterial community of freshwater ecosystems. Microbiology 82(4):459-465. Pure culture was maintained in liquid HCY medium containing Hutners basal salt solution (20 mL/L), a vitamin mixture (5), xylose (0.5 g/L) as a substrate, and yeast extract (0.01 g/L). Ref 5: Zaichikova, M.V., Berestovskaya, Akimov, V.N., Kizilova, A.K., and Vasileva, L.V., Xantobacter xylophilus sp. nov., a member of the xylotrophic myco-bacterial community of low mineral oligotrophic waters, Microbiology, 2010, vol. 79, no. 1, pp. 83-88. HCY agar medium A mineral-salts, solid microbiological culture medium containing magnesium sulfate and nitrilotriacetic acid. Used to support the growth of Spirosoma and Xanthobacter. Zobell marine medium, half-strength Carrine Blank An organic-rich, mineral-salts, liquid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Diluted by half with water. Used for the cultivation of heterotrophic marine or brackish microorganisms. dextrose broth From: Dextrose Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Dextrose Agar is used for cultivating a wide variety of microorganisms with or without added blood. Dextrose Broth is used for cultivating fastidious microorganisms and for detecting gas from enteric bacilli. Principles of the Procedure Beef extract and peptones provide nitrogen, amino acids and vitamins. Dextrose is a carbon source, and the increased concentration is a distinguishing characteristic of this medium from other formulations used as blood agar bases. Agar is the solidifying agent. Supplementation with 5% blood provides additional growth factors for fastidious microorganisms. Formulae Difco™ Dextrose Broth Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 5.0 g Proteose Peptone No. 3................................................ 2.0 g Pancreatic Digest of Gelatin......................................... 3.0 g Beef Extract.................................................................. 3.0 g Dextrose...................................................................... 5.0 g Sodium Chloride.......................................................... 5.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.2 ± 0.2 Glucose broth An organic-rich, liquid microbiological culture medium containing peptones and glucose. Used to culture a variety of microorganisms. Carrine Blank broth is pH 7.2 dextrose agar An organic-rich, solid microbiological culture medium containing peptones and glucose. Used to culture a variety of microorganisms. Carrine Blank Glucose agar From: Dextrose Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Dextrose Agar is used for cultivating a wide variety of microorganisms with or without added blood. Dextrose Broth is used for cultivating fastidious microorganisms and for detecting gas from enteric bacilli. Principles of the Procedure Beef extract and peptones provide nitrogen, amino acids and vitamins. Dextrose is a carbon source, and the increased concentration is a distinguishing characteristic of this medium from other formulations used as blood agar bases. Agar is the solidifying agent. Supplementation with 5% blood provides additional growth factors for fastidious microorganisms. Formulae Difco™ Dextrose Agar Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 5.0 g Proteose Peptone No. 3................................................ 2.0 g Pancreatic Digest of Gelatin......................................... 3.0 g Beef Extract.................................................................. 3.0 g Dextrose.................................................................... 10.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g pH 7.3 ± 0.2 pH is 7.3 Flexibacter maritimus medium agar An organic-rich, solid microbiological culture medium containing peptone, yeast extract, and acetate in a seawater base. Used to culture Flexibacter maritimus. Flexibacter maritimus medium Carrine Blank FMM agar medium From Table 2 in: Pazos F, Santos Y, Macias AR, Nunez S, & Toranzo AE. 1996. Evaluation of media for the successful culture of Flexibacter maritimus. Journal of Fish Diseases 19:196-197. Components (g/L) Peptone 5.0 Yeast extract 0.5 Sodium acetate 0.01 Agar 15 In sea water as the diluent pH 7.2-7.4 FMM agar McClung-Toabe agar egg-yolk agar From: McClung-Toabe Agar (Atlas, The Handbook of Microbiological Media for the Examination of Food, 2nd ed., pgs 221-222) Composition per liter: Proteose peptone 40.0g Agar 25.0g Na2HPO4 5.0 g Glucose 2.0 g NaCl 2.0 g KH2PO4 1.0 g MgSO4x7H2O 0.1 g Egg yolk emulsion, 50% 100.0 mL pH 7.3 +/- 0.2 at 25˚C Source: This medium is available as a pre-mixed powder from BD Diagnostic Systems. Egg Yolk Emulsion, 50%: Composition per 100.0 mL: Chicken egg yolks 11 Whole chicken egg 1 NaCl (0.9% solution) 50.0 mL Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100 dilution of saturated mercuric chloride solution for 1 min. Crack eggs and separate yolks from whites. Mix egg yolks with 1 chicken egg. Measure 50.0 mL of egg yolk emulsion and add to 50.0 mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize. Warm to 45-50˚C. Preparation of Medium: Add components, except egg yolk emulsion, 50%, to distilled/deionized water and bring volume to 900.0 mL. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure -121˚C. Cool to 50-55˚C. Aseptically add 100.0 mL of sterile egg yolk emulsion, 50%. Mix thoroughly. Pour into sterile Petri dishes in 15.0 mL volumes. Use: For the isolation and cultivation of Clostridium perfringens in foods. An organic-rich, liquid microbiological culture medium contining peptone, mineral-salts, glucose, and egg yolk emulsion. Used for the isolation and growth of Clostridium perfringens. Carrine Blank Eggerth-Gagnon agar An organic-rich, solid microbiological culture medium containing peptones, phosphate buffer, blood, and glucose. Used for the growth of Bacteroides sp. EG agar Carrine Blank EG blood agar From: Eggerth AH & Gagnon BH. 1932. The bacteroides of human feces. J Bacteriol 35(4):389-413. "Our basic medium was beef infusion agar, containing 1.5 per cent of agar, 1 per cent of Parke Davis peptone, and 0.4 per cent of di-sodium phosphate. The pH was 7.6 to 7.8. Before pouring the plates, about 5 per cent of sterile blood and 0.15 per cent of sterile glucose (in the form of a 10 per cent solution) were added." DNase test agar From: DNase Test Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use DNase Test Agar, DNase Test Agar with Methyl Green and DNase Test Agar with Toluidine Blue are differential media used for the detection of deoxyribonuclease activity to aid in the identification of bacteria isolated from clinical specimens. Summary and Explanation The DNase test is used to detect the degradation of deoxyribonucleic acid (DNA). The test is useful for differentiating Serratia from Enterobacter, Staphylococcus aureus from coagulase-negative staphylococci, and Moraxella catarrhalis from Neisseria species. Principles of the Procedure Peptones provide amino acids and other complex nitrogenous substances to support bacterial growth. Sodium chloride maintains osmotic equilibrium. DNA is the substrate for DNase activity. DNase is an extracellular enzyme that breaks the DNA down into subunits composed of nucleotides. The depolymerization of the DNA may be detected by flooding the surface of the medium with 1 N HCl and observing for clear zones in the medium surrounding growth. In the absence of DNase activity, the reagent reacts with the intact nucleic acid, resulting in the formation of a cloudy precipitate. The HCl reagent is not needed to detect DNase activity on DNase Agar with Methyl Green. Methyl green forms a complex with intact (polymerized) DNA to form the green color of the medium. DNase activity depolymerizes the DNA, breaking down the methyl green-DNA complex, which results in the formation of colorless zones around colonies of the test organism. A negative test is indicated by the absence of a colorless zone around the colonies. The HCl reagent is not needed to detect DNase activity on DNase Agar with Toluidine Blue. Toluidine blue forms a complex with intact (polymerized) DNA. In the intact DNA complex, the toluidine blue has the normal blue color. DNase activity depolymerizes the DNA, breaking down the dye-DNA complex. In the presence of nucleotides produced from the DNase depolymerization, the dye takes on its metachromatic color, forming pink to red zones around bacterial growth. A negative test is indicated when the medium remains blue. Formulae Difco™ DNase Test Agar Approximate Formula* Per Liter Tryptose..................................................................... 20.0 g Deoxyribonucleic Acid.................................................. 2.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g pH 7.3 ± 0.2 BBL™ DNase Test Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 15.0 g Papaic Digest of Soybean Meal..................................... 5.0 g Deoxyribonucleic Acid.................................................. 2.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g pH 7.3 ± 0.2 DNase agar Carrine Blank An organic-rich, solid microbiological culture medium containing tryptose and DNA. Used to test for the ability of a microorganism to degrade DNA. deoxycholate lactose agar Carrine Blank An organic-rich, solid microbiological culture medium containing peptone, lactose, citrate, deoxycholate, and neutral red. Used for the isolation of microorganisms that grow in milk (or other dairy products). Deoxycholate (a weak anionic detergent and bile acid) is used to lyse cells and solublize some cell membrane components. Inhibits growth of Gram positive bacteria, and therefore selects for Gram negative bacteria such as Salmonella. From: Deoxycholate Lactose Agar (M066, HiMedia) Deoxycholate Lactose Agar is a differential and slightly selective medium used for the isolation and enumeration of coliforms in water, wastewater, milk and dairy products. Composition** Ingredients Gms / Litre Peptone, special 10.000 Lactose 10.000 Sodium chloride 5.000 Sodium citrate 2.000 Sodium deoxycholate 0.500 Neutral red 0.030 Agar 15.000 Final pH ( at 25°C) 7.1±0.2 **Formula adjusted, standardized to suit performance parameters Directions Suspend 42.53 grams in 1000 ml distilled water. Mix well and heat to boiling to dissolve the medium completely. The medium requires no autoclaving if it is to be used at once. If the medium is to be stored, it should be sterilized at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING. deoxycholate lactose medium DSMZ Carrine Blank DSM strains: From: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf xxxx CSY-3 medium CSY-3 From: Sawabe T, Makino H, Tatsumi M, Nakano K, Tajima K, Iqbal MM, Yumoto I, Ezura Y & Christen R. 1998. Pseudoalteromonas bacteriolytica sp. nov., a marine bacterium that is the causative agent of red spot disease of Laminaria japonica. IJSB 48:769-774. "These isolated of Pseudoalteromonas bacteriolytica were maintained on CSY-3 agar medium containing casitone (Difco) 1.0 g, Difco bacto-soytone 1.0 g, Difco yeast extract 1.0 g, ferric ammonium citrate 0.1 g, and 1000 ml natural seawater, pH 7.5 (19). The stock cultures were maintained in CSY-3 broth containing 20 % glycerol (v/v)." Carrine Blank CSY-3 broth An organic-carbon enriched, marine, liquid microbiological culture medium containing peptones (casitone, soytone, yeast extract) and ferric ammonium citrate in a seawater base. Used for the cultivation of Pseudoalteromonas. medium containing cellulose or cellulose derivatives Carrine Blank A microbiological culture medium containing carboxymethyl cellulose. Used to screen a culture for cellulolytic activity (the ability to hydrolyze cellulose, particularly those with endoglucanases). chocolate agar http://medical-dictionary.thefreedictionary.com/Chocolate+Agar Blood agar that has been heated to open the pyrrole ring, forming hemin, a required growth factor for bacteria lacking hemolysins. Chocolate agars are usually incubated in a microaerophilic—3–10% CO2—environment, providing ideal growth conditions for H influenzae, Neisseria spp, and fastidious anaerobes Carrine Blank Wikipedia:Chocolate_agar Chocolate agar (CHOC) or chocolate blood agar (CBA) - is a non-selective, enriched growth medium. [1] [2] It is a variant of the blood agar plate, containing red blood cells that have been lysed by slowly heating to 80 °C. Chocolate agar is used for growing fastidious respiratory bacteria, such as Haemophilus influenzae and Neisseria meningitidis.[3] In addition, some of these bacteria, most notably H. influenza, need growth factors such NAD (factor V) and hemin (factor X), which are inside red blood cells; thus, a prerequisite to growth for these bacteria is lysis of the red blood cells. The heat also inactivates enzymes which could otherwise degrade NAD. The agar is named for the color and contains no actual chocolate. A solid microbiological culture medium where blood has been been treated by heating in order to lyse the blood cells. cetrimide agar Carrine Blank An organic-rich, solid microbiological culture medium that contains pancreatic digest of gelatin, potassium sulfate, and cetrimide (cetyl trimethyl ammonium bromide). A medium selective for Pseudomonas aeruginosa. From: Cetrimide Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Cetrimide (Pseudosel) Agar is used for the selective isolation and identification of Pseudomonas aeruginosa. Principles of the Procedure Gelatin peptone supplies the nutrients necessary to support growth. The production of pyocyanin is stimulated by the magnesium chloride and potassium sulfate in the medium. Cetrimide is a quaternary ammonium, cationic detergent compound, which is inhibitory to a wide variety of bacterial species including Pseudomonas species other than P. aeruginosa. Agar is a solidifying agent. Cetrimide Agar Base is supplemented with 1% glycerol as a source of carbon. Formula Difco™ Cetrimide Agar Base Approximate Formula* Per Liter Pancreatic Digest of Gelatin....................................... 20.0 g Magnesium Chloride.................................................... 1.4 g Potassium Sulfate....................................................... 10.0 g Cetrimide (Tetradecyltrimethylammonium Bromide)...... 0.3 g Agar.......................................................................... 13.6 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.2 ± 0.2 Directions for Preparation from Dehydrated Product 1. Suspend 45.3 g of the powder in 1 L of purified water containing 10 mL of glycerol. Mix thoroughly. 2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3. Autoclave at 121°C for 15 minutes. 4. Test samples of the finished product for performance using stable, typical control cultures. trace elements solution ho-le Carrine Blank A trace elements solution containing boric acid, manganese chloride, ferrous sulfate, sodium tartrate, copper chloride, zinc chloride, cobalt chloride, and sodium molybdate. From: http://www.atcc.org/~/media/DF06C418BB3A44189AD1A3CEB59B8D6E.ashx ATCC Medium: 1776 Tyrosine Agar (ISP Medium 7) Trace Elements Solution Ho-Le: H3BO3 2.85 g MnCl2x4H2O 1.8g FeSO4 1.36 g Sodium tartrate 1.77 g CuCl2x2H2O 26.9 mg ZnCl2 20.8 mg CoCl2x6H2O 40.4 mg Na2MoO4x2H2O 25.2 mg Distilled water 1.0 L Brucella medium From: Brucella Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Brucella Agar is a culture medium for the cultivation of Brucella organisms. With the addition of 5% horse blood, the medium is used in qualitative procedures for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical specimens. Brucella Broth is used for the cultivation of Brucella species and for the isolation and cultivation of a wide variety of fastidious and nonfastidious microorganisms. Principles of the Procedure Brucella Agar and Brucella Broth support the growth of fastidious microorganisms due to their content of peptones, dextrose and yeast extract. The peptones supply organic nitrogen. The yeast extract is a potent source of the B-complex vitamins. Dextrose is utilized as an energy source. Sodium bisulfite is a reducing agent, and sodium chloride maintains the osmotic equilibrium. Agar is the solidifying agent in Brucella Agar. Formulae BBL™ Brucella Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 10.0 g Peptic Digest of Animal Tissue.................................... 10.0 g Dextrose...................................................................... 1.0 g Yeast Extract................................................................ 2.0 g Sodium Chloride.......................................................... 5.0 g Sodium Bisulfite........................................................... 0.1 g Agar.......................................................................... 15.0 g BBL™ Brucella Broth Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2 BM BM medium An organic-rich, liquid microbiological culture medium, containing pancreatic digest of casein (casitone), peptic digest of animal tissue (proteose peptone), dextrose (D-glucose), yeast extract, sodium chloride, and sodium bisulfite (sodium hydrogensulfite) as a reducing agent. Used for the growth of Brucella. Carrine Blank Brucella agar An organic-rich, solid microbiological culture medium, containing pancreatic digest of casein (casitone), peptic digest of animal tissue (proteose peptone), dextrose (D-glucose), yeast extract, sodium chloride, sodium bisulfite (sodium hydrogensulfite) as a reducing agent, and agar. Used for the growth of Brucella. From: Brucella Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Brucella Agar is a culture medium for the cultivation of Brucella organisms. With the addition of 5% horse blood, the medium is used in qualitative procedures for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical specimens. Brucella Broth is used for the cultivation of Brucella species and for the isolation and cultivation of a wide variety of fastidious and nonfastidious microorganisms. Principles of the Procedure Brucella Agar and Brucella Broth support the growth of fastidious microorganisms due to their content of peptones, dextrose and yeast extract. The peptones supply organic nitrogen. The yeast extract is a potent source of the B-complex vitamins. Dextrose is utilized as an energy source. Sodium bisulfite is a reducing agent, and sodium chloride maintains the osmotic equilibrium. Agar is the solidifying agent in Brucella Agar. Formulae BBL™ Brucella Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 10.0 g Peptic Digest of Animal Tissue.................................... 10.0 g Dextrose...................................................................... 1.0 g Yeast Extract................................................................ 2.0 g Sodium Chloride.......................................................... 5.0 g Sodium Bisulfite........................................................... 0.1 g Agar.......................................................................... 15.0 g BBL™ Brucella Broth Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2 Carrine Blank BM agar Ayers casein agar Ayers' agar medium Ayer's agar An organic-rich, solid microbiological culture medium containing casein and agar. Used to study microorganisms capable of growth in milk. Ayer's casein agar Carrine Blank From: Ayers, SH. 1911. CASEIN MEDIA ADAPTED TO THE BACTERIAL EXAMINATION OF MILK. in U.S. Department of Agriculture. 28th annual report of the Bureau of Animal Industry. PREPARATION OF CASEIN-AGAR The method of preparation of casein-agar is as follows: PREPARATION OF ONE LITER. Casein solution 300 c.c. distilled water, 10 grams casein (Eimer & Amend C.P. casein prepared according to Hammarstan), 7 c.c. normal sodium hydroxid. After dissolving casein make up to 500 cubic centimeters. Agar solution. 500 c.c. distilled water, 10 grams agar. To 300 c.c. of water (distilled) add 10 grams casein (Eimer & Amend C.P. casein prepared according to Hammarsten) and 7 c.c. normal sodium hydroxid. Dissolve casein by heating to boiling. It is desirable to let this stand for several hours to get a perfect solution. This is not necessary, however. Make up volume to 500 c.c., and bring the reaction of the solution to between +0.1 and +0.2, Fuller's scale. Do not allow solution to become alkaline to phenolphthalein or over +0.2. If the casein is weighed accurately and the normal solution is accurate the reaction will be about +0.2. The agar solution is prepared by dissolving 10 grams of agar in 500 c.c. of water. Both casein and agar solutions should be filtered, then mixed. Tube and sterilize in autoclave under pressure for 20 minutes; then cool the tubes quickly in cold water or ice water. The final reaction of the medium will be about +0.1, Fuller's scale. IF the medium is alkaline the bacterial growth will be restricted. If the medium is more than +0.1 some of the casein may be precipitated during sterilization. The casein-agar should be clear and almost colorless when poured into a Petri dish. Sometimes the casein will be slightly precipitated during sterilization or the cooling, but this is of no consequence, since on pouring into plates the precipitate on account of its finely divided condition, becomes invisible. Anacker and Ordal medium Carrine Blank A dilute, organic-carbon-containing liquid microbiological culture medium containing acetate, tryptone, and extracts of yeast and beef. Used for the cultivation and maintenance of Flexibacter psychrophilus. Anacker and Ordal broth AOB ATCC medium 1750: Anacker and Ordal medium Tryptone (BD 211705).........0.5 g Yeast extract................0.5 g Sodium acetate...............0.2 g Beef extract.................0.2 g Agar........................10.0 g Distilled water..............1.0 L Adjust medium for final pH 7.3 +/- 0.1. Autoclave at 121C for 15 minutes. (For the broth the agar would be omitted). Anacker-Ordal broth Anacker and Ordal agar Carrine Blank AOA Anacker & Ordal's agar A dilute, organic-carbon-containing solid microbiological culture medium containing acetate, tryptone, and extracts of yeast and beef. Used for the cultivation and maintenance of Flexibacter psychrophilus. Anacker-Ordal agar ATCC medium 1750: Anacker and Ordal medium Tryptone (BD 211705).........0.5 g Yeast extract................0.5 g Sodium acetate...............0.2 g Beef extract.................0.2 g Agar........................10.0 g Distilled water..............1.0 L Adjust medium for final pH 7.3 +/- 0.1. Autoclave at 121C for 15 minutes. (For the broth the agar would be omitted). ammonium mineral salts agar Carrine Blank A mineral-salts, solid microbiological culture medium for the growth of freshwater and weakly brackish microorganisms that utilize methanol as a source of carbon. From Handbook of Microbiological Media, 3rd edition: Composition per liter: Agar, 15.0 g MgSO4*7H2O, 1.0 g K2HPO4, 0.7 g KH2PO4, 0.54 g NH4Cl, 0.5 g CaCl2*2H2O, 0.2 g FeSO4*7H2O,4.0 mg H3BO4, 0.3 mg CoCl2*6H2O, 0.2 mg ZnSO4*7H2O, 0.1 mg Na2MoO4*4H2O, 0.06 mg MnCl2*4H2O, 0.03 mg NiCl2*6H2O, 0.02 mg CuCl2*2H2O, 0.01 mg pH 6.8 +/- 0.2 at 25˚C Preparation of Medium: Add components to distilled/deinionezed water and bring volume to 1.0 L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121˚C. Add sterile methanol to a concentration of 0.5% aseptically to cooled basal medium. Use: for the cultivation and mantenance of bacteria that can utilize methanol as a carbon source, such as Methylobacterium species, Methylomonas species, and Methylophilus species. AMS agar blood agar with washed red cells Agar plates made up with washed red cells A solid microbiological culture medium containing blood, where the red blood cells have been washed with a sterile saline solution. From: http://medical-dictionary.thefreedictionary.com/washed+red+cells Washed Red Cells Transfusion medicine RBCs that have been washed in sterile saline to remove WBCs, lytic mediators, non-self antigens; WRCs are most useful in IgA-deficient Pts who have circulating anti-IgA antibodies, used to ↓ febrile, urticarial and anaphylactic reactions. See Blood filters. Agar made up with washed red cells Carrine Blank Berkefeld filterable cell Wikipedia:Berkefeld_filter A Berkefeld filter[1] is a oil filter made of diatomaceous earth or Kieselguhr. It was invented in Germany in 1891, and by 1922 was being marketed in the United Kingdom by the Berkefeld Filter Co.[2] Berkefeld was the name of owner of the mine in Hanover, Germany, where the ceramic material was obtained. The Berkefeld is a good bacterial water filter used in microbiological laboratories, in homes and out in the field.[3][4] Types The filters are classified according to the diameter of the pores in the ceramic material: V (Viel) - Coarsest pores N (Normal) - Intermediate sized pores W (Wenig) - Finest pores Usefulness The Berkefeld is a cheap, portable and efficient bacterial filter in general, though it does not remove viruses like Hepatitis A and some bacteria such as mycoplasma.[6] Some companies claim that they filter out from between 100% of particles above 0.9 micrometre to 98% of particles above 0.5 micrometre in diameter.[5] These are very durable filters and the filter elements may be cleaned over 100 times before requiring replacement. Some of the first Berkefeld filters were used during the 1892 cholera epidemic in Hamburg.[7] A prokaryotic cell which has a filterable cell size that is small enough to pass through a Berkefeld filter candle with a defined pore size (e.g. V, N, or W), the largest of which is 0.45 microns. Carrine Blank From: Mudd S. 1923. The penetration of bacteria through capillary spaces. I. Motility and Size as Influencing Filterability through Berkefeld Candles. J Bacteriol 8(5):459-481. Table 4 (pg. 471) FILTER CALCULATED DIAMETER AVERAGE DIAMETER FOR FILTER TYPE micron micron V (10) 0.414 V (11) 0.380 V (16) 0.373 0.38 V (17) 0.376 V (18) 0.371 N (X) 0.461 N (XI) 0.437 0.45 W (1) 0.524 W (2) 0.47A W (X) 0.369 0.43 W (XI) 0.343 Berkefeld filter Berkefeld V filterable cell 0.38 0.38 A Berkefeld filterable cell that is small enough to pass through a Berkefeld filter candle with a smaller pore size V (for Viel). Pore sizes average 0.38 microns in diameter. This is the most common filter size used to study the filterability of microorganisms. Berkefeld V Carrine Blank Berkefeld V filter Berkefeld N filterable cell 0.43 0.43 Carrine Blank Berkefeld N A Berkefeld filterable cell that is small enough to pass through a Berkefeld filter candle with an intermediate pore size N (for Normal). Pore sizes average 0.43 microns in diameter. Berkefeld N filter Berkefeld W filterable cell 0.45 0.45 Carrine Blank Berkefeld W filter A Berkefeld filterable cell that is small enough to pass through a Berkefeld filter candle with a wider pore size W (for Wenig). Pore sizes average 0.45 microns in diameter. Berkefeld W bile esculin agar Aesulin-ferric citrate An organic-rich, solid microbiological culture medium containing pancreatic digest of gelatin, beef extract, oxgall, esculin, and ferric iron. Used for the differentiation of enterococci, Streptococcus bovis, and other streptococci, Bile aesculin agar Carrine Blank Esculin ferric citrate From: Bile Esculin Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Bile Esculin Agar is used to differentiate enterococci and the Streptococcus bovis group from other streptococci. Principles of the Procedure Enterococci and certain streptococci hydrolyze the glycoside esculin to esculetin and dextrose. Esculetin reacts with an iron salt to form a dark brown or black complex.6 Ferric citrate is incorporated into the medium as an indicator of esculin hydrolysis and resulting esculetin formation. Oxgall is used to inhibit gram-positive bacteria other than enterococci. Formula BBL™ Bile Esculin Agar Approximate Formula* Per Liter Pancreatic Digest of Gelatin......................................... 5.0 g Beef Extract.................................................................. 3.0 g Oxgall........................................................................ 20.0 g Ferric Citrate............................................................... 0.5 g Esculin......................................................................... 1.0 g Agar ......................................................................... 14.0 g pH 6.8 ± 0.2 From: Wikipedia:Bile_esculin_agar Bile Esculin Agar (BEA) is a selective differential agar used to isolate and identify members of the genus Enterococcus, also known as "group D streptococci". Composition and process Bile salts are the selective ingredient, while esculin is the differential component. Enterococcus hydrolyze esculin to products that react with ferric citrate in the medium to produce insoluble iron salts, resulting in the blackening of the medium. Test results must be interpreted in conjunction with gram stain morphology. Uses Bile Esculin Agar is used primarily to differentiate Enterococcus from Streptococcus. Members of the genus Enterococcus are capable of growing in the presence of 4% bile (oxgall) and hydrolyzing esculin to glucose and esculetin. Esculetin combines with ferric ions to produce a black complex. For some purposes, certain bacteria are able to hydrolyze aesculin. A plate containing aesculin will fluoresce a pale blue under UV radiation. Some bacteria can hydrolise this, leading to UV dark colonies, as opposed to UV light ones. When new techniques are produced to identify enterococci, they are often compared to the use of bile esculin agar. casitone casein peptone, tryptic digest Bacto Casitone pancreatic digest of casein casein peptone A pancreatic digest of casein (milk protein from cow's milk, Bos taurus), used for the culturing of microorganisms. Carrine Blank From Bacto™ Casitone (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Bacto™ Casitone is a pancreatic digest of casein. The manufacturing process for an enzymatic digest of casein is not as destructive as an acid hydrolysis. Thus, the casein is not broken down as completely into its constituent components. In many cases this makes for a more nutritious hydrolysate, especially for those organisms that prefer peptides to amino acids. Ash content is 6.4% NaCl content is 0.0% meat infusion agar From: http://himedialabs.com/TD/M883.pdf Meat Infusion Agar (Standard Infusion Agar) M883 (HiMedia Laboratories) Meat Infusion Agar is a nutritive medium used for mass cultivation of organisms for vaccine or toxin production. Composition** Ingredients Gms/Litre Peptic digest of animal tissue 10.000 Beef, infusion from 500.000 Sodium chloride 5.000 Agar 25.000 Final pH (at 25˚C) 7.5 +/- 0.2 ** Formula adjusted, standardized to suit performance parameters. Standard infusion agar An organic-rich, solid microbiological culture medium containing peptic digest of animal tissue, beef infusion, and sodium chloride. Carrine Blank ox gall Gall (bile stored in the gall bladder) derived from an ox (a castrated adult male bovine; Bos taurus), used for the culturing of microorganisms. From Wikipedia:Ox_gall: Ox gall is gall, usually obtained from cows, that is mixed with alcohol and used as the wetting agent in paper marbling, engraving, lithography, and watercolor painting. It is a greenish-brown liquid mixture containing cholesterol, lecithin, taurocholic acid, and glycocholic acid. Carrine Blank Oxgall ox bile salts Carrine Blank Bile salts derived from the bile of an ox (a castrated adult male bovine; Bos taurus). Used in the cultivation of microorganisms. Ox-bile salts ox bile Carrine Blank Bile derived from an ox (a castrated adult bovine; Bos taurus). Used for the culturing of microorganisms. chopped meat meat Undefined mixtures of complex organic compounds derived from the chopped meat (muscle and fat) of mammals, used in the cultivation of microorganisms. Carrine Blank corn oil Oil extracted from the germ (the plant embryo) of corn (Zea mays), used in the cultivation of microorganisms. Carrine Blank homogenized chicken egg yolk Microbiological medium ingredient, derived from chicken egg, where the yolks of chicken eggs have been homogenized (mixed). Carrine Blank Loeffler blood serum medium An organic-rich, liquid microbiological culture medium that contains beef serum, heart infusion, whole egg, and dextrose. Used for the cultivation of Corynebacteria. https://www.bd.com/ds/technicalCenter/inserts/L007463(07)(0107).pdf pH is 7.6 +/- 0.2. Lijffler's blood serum Loeffler's medium Loeffler blood serum Wikipedia:Loeffler's_medium Löffler's medium is a special substance used to grow diphtheria bacilli to confirm the diagnosis. History In 1887, Friedrich Loeffler devised a culture medium containing horse serum, meat infusion, and dextrose for use in the cultivation of corynebacteria and for differentiating them from other organisms.[1] Perry and Petran suggested modification of the original formulation.[2] Buck, in 1949, described a modified Loeffler's medium for cultivating Corynebacterium diphtheriae.[3] Uses This medium has a variety of uses in microbiological investigations. The current formulation incorporated these later modifications: The primary value of Loeffler medium is in the growth and morphological characterization of members of the genus Corynebacterium. This formulation enhances the formation of metachromatic granules within the cells of the organisms. Due to its serum content, Loeffler medium can be used for the determination of proteolytic activities of microorganisms. The gray-white surface of the medium provides an excellent background for the detection and observation of colonial pigmentation. If all extraneous moisture is removed aseptically from the slants and the upper part of the slant is heated until the slant ruptures, this medium can be used for the detection of ascospores. Principles of the procedure Heart muscle and animal tissue peptone provide the amino acids and other complex nitrogenous substances necessary to support growth of corynebacteria. Sodium chloride supplies essential ions. Dextrose is a source of fermentable carbohydrate. The eggs and beef serum cause the medium to coagulate during the sterilization process and are sources of protein which are used for metabolism of the corynebacteria and other organisms. Approximate formula Per liter purified water Beef serum 70.0 g Heart muscle, infusion from (solids) 0.72 g Peptic digest of animal tissue 0.71 g Sodium chloride 0.36 g Dextrose 0.71 g Egg (whole, dried) 7.5 g Carrine Blank anaerobic respiration, by disproportionation of thiosulfate Carrine Blank The process of anaerobic respiration, where thiosulfate is both oxidized to sulfate and reduced to hydrogen sulfide. Gram stain positive Wikipedia: Gram-positive bacteria A gram stain quality where microorganisms stain purple with crystal violet used in the Gram stain. Carrine Blank Gram stain positive Gram reaction positive Gram positive Gram staining positive Gram stain results are positive Gram stain negative Gram stain results are negative Gram negative Wikipedia:Gram-negative bacteria Gram-staining-negative A gram stain quality where microorganisms do not stain purple with Crystal Violet used in the Gram stain, but stain red due to uptake of the counter stain (Safranin or Fuchsin). Fuchsin is also called Magenta. Gram stain negative Carrine Blank Gram reaction negative Gram stain variable Gram reaction variable Gram indeterminate Microorganisms that are not definitively stained Gram positive or Gram negative; may stain variably as either Gram positive or Gram negative. Gram stain results are variable Carrine Blank Gram-staining-variable Gram stain variable Gram staining variable Gram variable magnetotaxis Wikipedia:Magnetotaxis Carrine Blank Movement (taxis) of a microorganism along the Earth's magnetic field lines. methyl red assay methyl red test Wikipedia: IMViC The methyl red test detects production of acids formed during metabolism using mixed acid fermentation pathway using pyruvate as a substrate. The pH indicator Methyl Red is added to one tube and a red color appears at pH's lower than 4.2, indicating a positive test (mixed acid fermentation is used). The solution remaining yellow (pH = 6.2 or above) indicates a negative test, meaning the butanediol fermentation is used. methyl red Carrine Blank Assays for the production of acids (e.g. pyruvate, lactate, acetate, formate) during the fermentation of glucose. The dye methyl red is red below pH 4.4 and yellow above pH 6.2. Acidic fermentation products result in a positive MR test giving a red result. A negative result is yellow. Wikipedia: Mixed acid fermentation Mixed acid fermentation is the biological process by which a six-carbon sugar e.g. glucose is converted into a complex and variable mixture of acids. It is an anaerobic fermentation reaction that is common in bacteria. It is characteristic for members of the Enterobacteriaceae, a large family of Gram-negative bacteria that includes E. coli.[3] The mixture of end products produced by mixed acid fermentation includes lactate, acetate, succinate, formate, ethanol and the gases H2 and CO2. The formation of these end products depends on the presence of certain key enzymes in the bacterium. The proportion in which they are formed varies between different bacterial species.[4] The mixed acid fermentation pathway differs from other fermentation pathways, which produce fewer end products in fixed amounts. The end products of mixed acid fermentation can have many useful applications in biotechnology and industry. For instance, ethanol is widely used as a biofuel.[5] Therefore, multiple bacterial strains have been metabolically engineered in the laboratory to increase the individual yields of certain end products.[2] This research has been carried out primarily in E. coli and is ongoing. MR test methylene blue assay An assay to determine if methylene blue can act as an artificial donor of electrons to cytochrome oxidase. When methylene blue is reduced it turns from oxidized blue form to the reduced colorless form. Methylene blue inhibits the growth of gram-positive bacteria, therefore inclusion in agar media is used to select for gram-negative bacteria. Carrine Blank Wikipedia:Methylene_blue http://www.microbelibrary.org/component/resource/laboratory-test/2869-eosin-methylene-blue-agar-plates-protocol phenylethyl alcohol agar An organic-rich, solid microbiological culture medium containing peptones, sodium chloride, and phenylethyl alcohol. Is a selective media that inhibits the growth of Gram-negative bacteria, and thus is selective for Gram-positive bacteria. From: http://www.microbelibrary.org/library/laboratory-test/3653-phenylethyl-alcohol-agar-protocol Phenylethyl alcohol (bezylcarbinol) inhibits most gram-negative bacteria and fungi. From: Phenylethyl Alcohol Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Phenylethyl Alcohol (PEA) Agar is a selective medium for the isolation of gram-positive organisms, particularly gram-positive cocci, from specimens of mixed gram-positive and gram-negative flora.1 The medium, when supplemented with 5% sheep blood, should not be used for determination of hemolytic reactions since atypical reactions may be observed. Principles of the Procedure Phenylethyl Alcohol Agar and Phenylethyl Alcohol Agar with 5% Sheep Blood support the growth of gram-positive bacterial species, due to the content of peptones, which supply nitrogen, carbon, sulfur and trace nutrients. Sodium chloride maintains osmotic equilibrium. Sheep blood is a source of growth factors. Phenylethyl alcohol is bacteriostatic for gramnegative bacteria since it selectively and reversibly inhibits DNA synthesis.3 Formula BBL™ Phenylethyl Alcohol Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 15.0 g Papaic Digest of Soybean Meal..................................... 5.0 g Sodium Chloride.......................................................... 5.0 g β-Phenylethyl Alcohol................................................... 2.5 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.2 PEA Carrine Blank PAA mannitol salt agar From: Mannitol Salt Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Mannitol Salt Agar is used for the selective isolation and enumeration of staphylococci from clinical and nonclinical materials. Principles of the Procedure Mannitol Salt Agar is a nutritive medium due to its content of peptones and beef extract, which supply essential growth factors, such as nitrogen, carbon, sulfur and trace nutrients. The 7.5% concentration of sodium chloride results in the partial or complete inhibition of bacterial organisms other than staphylococci. Mannitol fermentation, as indicated by a change in the phenol red indicator, aids in the differentiation of staphylococcal species. Agar is a solidifying agent. Formula BBL™ Mannitol Salt Agar Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 5.0 g Peptic Digest of Animal Tissue...................................... 5.0 g Beef Extract.................................................................. 1.0 g Sodium Chloride........................................................ 75.0 g D-Mannitol................................................................ 10.0 g Phenol Red................................................................ 25.0 mg Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.4 ± 0.2 A hypersaline, organic-rich, solid microbiological culture medium that contains peptones, 7.5% sodium chloride, D-mannitol, and a pH indicator (phenol red). Mannitol fermentation lowers the pH, causing the red pH indicator to turn yellow. Used to distinguish between strains of Staphylococcus. Carrine Blank MSA methylene blue reduction assay methylene blue reduction Methylene blue acts as an artificial donor of electrons to cytochrome oxidase. When reduced it turns from oxidized blue form to the reduced colorless form. Carrine Blank methylene blue inhibition assay methylene blue inhibition Carrine Blank Methylene blue inhibits the growth of gram-positive bacteria, therefore inclusion in agar media is used to select for gram-negative bacteria. eosin methylene blue agar Carrine Blank From: Eosin Methylene Blue Agar, Levine (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Eosin Methylene Blue Agar, Levine is a slightly selective and differential plating medium for the isolation of gram-negative enteric bacteria. EMB Agar, Levine, without Lactose is provided for convenience in genetic studies of enteric bacilli. Principles of the Procedure The eosin Y and methylene blue dyes in Levine EMB Agar render the medium slightly selective in that they inhibit gram-positive bacteria to a limited degree. These dyes also play a role in differentiating between lactose fermenters and lactose nonfermenters due to the presence or absence of dye uptake in the bacterial colonies. Coliforms, as lactose-fermenting organisms, are visualized as blue-black colonies, whereas colonies of Salmonella and Shigella, as lactose nonfermenters, appear colorless, transparent or amber. Some gram-positive bacteria, such as fecal streptococci, staphylococci and yeasts, will grow on this medium and usually form pinpoint colonies. A number of nonpathogenic, lactose-nonfermenting gram-negative bacteria will grow on this medium and must be distinguished from the pathogenic strains by additional biochemical tests. Formulae BBL™ Eosin Methylene Blue Agar, Levine Approximate Formula* Per Liter Pancreatic Digest of Gelatin....................................... 10.0 g Lactose...................................................................... 10.0 g Dipotassium Phosphate................................................ 2.0 g Eosin Y........................................................................ 0.4 g Methylene Blue.......................................................... 65.0 mg Agar.......................................................................... 15.0 g BBL™ EMB Agar, Levine, without Lactose Consists of the same ingredients without the lactose. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.1 ± 0.2 EMB agar An organic-rich, solid microbiological culture medium containing pancreatic digest of gelatin, lactose, and dyes (Eosin Y and Methylene Blue) as selective agents. The dyes slightly inhibit the growth of Gram-positive bacteria. They are also used to differentiate lactose fermenters from those that do not ferment lactose based on the uptake of dye (lactose fermenters take up dye and appear blue-black). IMViC test Carrine Blank Wikipedia:IMViC A group of diagnostic metabolic tests used to differentiate coliform microorganisms. Includes indole test, methyl red test, Voges-Proskauer test, and citrate test. Voges-Proskauer assay Wikipedia:Voges–Proskauer_test v-p VP acetylmethylcarbinol VP acetoin Carrine Blank Voges-Proskauer An assay used to test for the production of acetoin by a microorganism as a product of glucose metabolism. Voges-Proskauer broth (containing alpha-naphthol and KOH) is inoculated with the test strain. A red color indicates a positive result for acetoin; absence of acetoin yields a yellow-brown color and indicates a negative result. acetoin production Voges-Proskauer test citrate fermentation/oxidation assay citrate utilization test Simmons citrate test citrate test Carrine Blank Simmons' citrate test Uses Simmons' citrate agar containing citrate. Microorganisms able to metabolize citrate change the pH of the medium through the production of sodium bicarbonate and ammonia, resulting in an alkaline pH (resulting in a change in the pH indicator, bromthymol blue). A positive reaction (alkaline pH) results in a blue (or blue-green) color; a negative reaction (neutral pH) results in a green (or pale green/yellow) color. CIT Wikipedia:Citrate_test citrate utilization MR-VP medium Methyl-red Voges-Proskauer broth An organic-rich, liquid medium that contains peptones, glucose, and a phosphate pH buffer. After growth, the Methyl Red test is performed by adding methyl red and looking for a color change. If glucose is fermented to acid sufficient to overcome the pH buffer, a red color will result. After growth, the Voges-Proskauer test is performed by adding KOH and alpha-naphthol to the culture. If acetoin was produced from glucose fermentation, a red color will result. Voges-Proskaeur Broth From: MR-VP Medium (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use MR-VP Medium and MR-VP Broth (Methyl Red-Voges Proskauer Medium/Broth, also known as Buffered Peptone- Glucose Broth) are used for the differentiation of bacteria by means of the methyl red and Voges-Proskauer reactions. Principles of the Procedure Methyl red-positive organisms produce high levels of acid during fermentation of dextrose, overcome the phosphate buffer system and produce a red color upon the addition of the methyl red pH indicator. In the Voges-Proskauer test, the red color produced by the addition of potassium hydroxide to cultures of certain microbial species is due to the ability of the organisms to produce a neutral end product, acetoin (acetylmethylcarbinol), from the fermentation of dextrose.3 The acetoin is oxidized in the presence of oxygen and alkali to produce a red color.3 This is a positive Voges-Proskauer reaction. Formulae Difco™ MR-VP Medium Approximate Formula* Per Liter Buffered Peptone......................................................... 7.0 g Dipotassium Phosphate................................................ 5.0 g Dextrose...................................................................... 5.0 g pH 6.9 ± 0.2 BBL™ MR-VP Broth Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 3.5 g Peptic Digest of Animal Tissue...................................... 3.5 g Potassium Phosphate................................................... 5.0 g Dextrose...................................................................... 5.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.9 ± 0.2 Methyl-red Voges-Proskauer medium Carrine Blank Simmons citrate agar Simmons' medium A mineral-salts, solid microbiological culture medium containing phosphate buffer, sodium chloride, sodium citrate, magnesium sulfate, and a pH indicator (bromthymol blue). Used to test for the metabolism of citrate. Carrine Blank Simmons' citrate medium From: Simmons Citrate Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Simmons Citrate Agar is used for the differentiation of gramnegative bacteria on the basis of citrate utilization. Principles of the Procedure Organisms able to utilize ammonium dihydrogen phosphate and sodium citrate as the sole sources of nitrogen and carbon, respectively, will grow on this medium and produce an alkaline reaction as evidenced by a change in the color of the bromthymol blue indicator from green (neutral) to blue (alkaline). Formula BBL™ Simmons Citrate Agar Approximate Formula* Per Liter Ammonium Dihydrogen Phosphate.............................. 1.0 g Dipotassium Phosphate................................................ 1.0 g Sodium Chloride.......................................................... 5.0 g Sodium Citrate............................................................. 2.0 g Magnesium Sulfate...................................................... 0.2 g Agar.......................................................................... 15.0 g Bromthymol Blue......................................................... 0.08 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.9 ± 0.2 Simmons medium freshwater microbiological culture medium Carrine Blank A microbiological culture medium that has a salinity of less than 0.05 % salts. sulfide indole motility agar motility medium An organic-rich, liquid microbiological culture medium containing peptones, ferrous iron, and thiosulfate. Used to test for the production of sulfide and indole, and motility in a microorganism. SIM tube sulfide indole motility medium Carrine Blank SIM medium From: http://www.microbelibrary.org/library/2-associated-figure-resource/3645-sim-medium Sulfide Indole Motility Medium Sulfide indole motility (SIM) medium is a semisolid agar used to determine hydrogen sulfide (H2S) production, indole formation, and motility. SIM medium is used to differentiate members of the family Enterobacteriaceae. Haziness that spreads from the stab line indicates a positive test for motility. Tubes must be compared to an uninoculated tube to discriminate between faint haziness and motility. A red color development after addition of Kovács reagent indicates indole production. A black precipitate indicates H2S production. Test tubes: (A) uninoculated tube, (B) contains the nonmotile and indole-negative bacterium Klebsiella pneumoniae, (C) contains the motile and indole-positive bacterium Escherichia coli, and (D) contains the motile, indole-negative, and H2S-producing bacterium Proteus mirabilis. (Renee Wilkins, University of Mississippi Medical Center, Jackson, MS) From: SIM Medium (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use SIM Medium is used to differentiate enteric bacilli on the basis of sulfide production, indole formation and motility. Principles of the Procedure The ingredients in SIM Medium enable the determination of three activities by which enteric bacteria can be differentiated. Sodium thiosulfate and ferrous ammonium sulfate are indicators of hydrogen sulfide production. The ferrous ammonium sulfate reacts with H2S gas to produce ferrous sulfide, a black precipitate.1 The casein peptone is rich in tryptophan, which is attacked by certain microorganisms resulting in the production of indole. The indole is detected by the addition of chemical reagents following the incubation period. Motility detection is possible due to the semisolid nature of the medium. Growth radiating out from the central stab line indicates that the test organism is motile. Formula BBL™ SIM Medium Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 20.0 g Peptic Digest of Animal Tissue...................................... 6.1 g Ferrous Ammonium Sulfate.......................................... 0.2 g Sodium Thiosulfate...................................................... 0.2 g Agar............................................................................ 3.5 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.2 sulfide indole mobility assay SIM test mobility agar An assay to determine hydrogen sulfide production, indole production, and motility of a microorganism. For the assay, an inoculum is made into sulfide indole motility (SIM) medium, an agar medium allowing three simultaneous assays to be performed: 1) hydrogen sulfide production due to cyteine desulfurase (indicated by a black precipitate) - the hydrogen sulfide test 2) indole production due to tryptophanase (indicated by red color development due to Kovacs reagent in the indole test) - the indole test 3) motility (spread of microbial cells away from the inoculum) Carrine Blank filament branch shape Carrine Blank Filament branch quality defining the shape of the filament branch. branch diameter thickness relative to main filament Filament branch length defining the filament branch thickness relative to that of the main filament. Carrine Blank branches thicker than main filament Carrine Blank Filament branch size where the diameter of cylindrical cells in the filament branches are thinner than the diameter of cylindrical cells in the main trichome. spatially differentiated branches Filament branch physical object quality relating to the spatial pattern (symmetry and position) of branches in relation with each other and with the long axis of the main filament, imparting some symmetry or asymmetry to the filament. Carrine Blank angled branches Carrine Blank Filament branch spatial pattern defining the angle between a filament (i.e., the main trichome) and a filament branch. branch subperpendicular to trichome Filament branch angle, where the angle between the cylindrical cells of the branching filament and the cylindrical cells of the main trichome is subperpendicular (forming an obtuse angle). Carrine Blank branch subparallel to trichome Carrine Blank Filament branch angle, where the angle between the cylindrical cells of the branching filament and the cylindrical cells of the main trichome is subparallel (forming an acute angle). urea medium From: Urea Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Urea Agar and Urease Test Broth are used for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production. Principles of the Procedure The urea medium of Rustigian and Stuart3 is particularly suited for the differentiation of Proteus species from other gramnegative enteric bacilli capable of utilizing urea;1 the latter are unable to do so in Urease Test Broth because of limited nutrients and the high buffering capacity of the medium. To provide a medium with greater utility, Urea Agar was devised by Christensen1 with peptone and dextrose included and reduced buffer content to promote more rapid growth of many of the Enterobacteriaceae and permit a reduction in incubation time. The complete Urea Agar contains 15.0 g/L of agar in addition to the ingredients in the base medium. When organisms utilize urea, ammonia is formed during incubation which makes the reaction of these media alkaline, producing a red-pink color. Consequently, urease production may be detected by the change in the phenol red indicator. Formulae Difco™ Urea Broth Approximate Formula* Per Liter Yeast Extract................................................................ 0.1 g Monopotassium Phosphate.......................................... 9.1 g Dipotassium Phosphate................................................ 9.5 g Urea........................................................................... 20.0 g Phenol Red.................................................................. 0.01 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.8 ± 0.1 Carrine Blank Stuart's urea broth An organic-rich, liquid microbiological culture medium containing pancreatic digest of gelatin, dextrose (D-glucose), sodium chloride, phosphate, urea, and a pH indicator (phenol red). urea broth urea agar An organic-rich, solid microbiological culture medium containing pancreatic digest of gelatin, dextrose (D-glucose), sodium chloride, phosphate, urea, a pH indicator (phenol red) and agar. Christensen's urea agar Carrine Blank From: Urea Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Urea Agar and Urease Test Broth are used for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production. Principles of the Procedure The urea medium of Rustigian and Stuart3 is particularly suited for the differentiation of Proteus species from other gramnegative enteric bacilli capable of utilizing urea;1 the latter are unable to do so in Urease Test Broth because of limited nutrients and the high buffering capacity of the medium. To provide a medium with greater utility, Urea Agar was devised by Christensen1 with peptone and dextrose included and reduced buffer content to promote more rapid growth of many of the Enterobacteriaceae and permit a reduction in incubation time. The complete Urea Agar contains 15.0 g/L of agar in addition to the ingredients in the base medium. When organisms utilize urea, ammonia is formed during incubation which makes the reaction of these media alkaline, producing a red-pink color. Consequently, urease production may be detected by the change in the phenol red indicator. Formulae BBL™ Urea Agar Base Approximate Formula* Per Liter Pancreatic Digest of Gelatin......................................... 1.0 g Dextrose...................................................................... 1.0 g Sodium Chloride.......................................................... 5.0 g Potassium Phosphate................................................... 2.0 g Urea........................................................................... 20.0 g Phenol Red................................................................ 12.0 mg pH 6.8 ± 0.1 oxidase assay cytochrome c oxidase OX Carrine Blank Assay for the presence of cytochrome oxidase (aka indophenol oxidase) in a microbiological culture. Uses a colorometric dye (tetra-methyl-p-phenylenediamine) as an electron donor for cytochrome c. When reduced the dye is colorless, when oxidized by cytochrome oxidase it becomes dark blue/purple. There are many variations of the test. It can be done on treated filter paper, on plates, or in a test tube using a fresh culture grown in broth. cytochrome c oxidase test http://www.microbelibrary.org/library/laboratory-test/3229-oxidase-test-protocol cytochrome oxidase Kovacs oxidase Kovac's oxidase kovacs oxidase test oxidase indophenol oxidase nitrate reduction assay NO3a Test for the presence of nitrate reductase in a microbial culture. Nitrite reacts with sulfanilic acid under acidic conditions (5N acetic acid) to produce (colorless) diazotized sulfanilic acid. This sulfanilic acid is then reacted to (colorless) alpha-naphthylamine (or N,N-dimethyl-alpha-naphthylamine) producing a red water soluble azo dye (p-sulfobenzene-azo alpha-naphthylamine). Presence of nitrite then results in a pink/red color (a positive test result). Zinc dust can be used to chemically (abiotically) reduce any nitrate remaining in the test to nitrite (to test for the presence of unreduced nitrate in the medium). If nitrate has been completely reduced to nitrogen or ammonia, zinc dust will result in no color development. This is a postive test result. However, if nitrate was not reduced and remains in the media, zinc will reduce it to nitrite resulting in a red color. This is a negative test result. NO3 Carrine Blank nitrate assimilation nitrate reduction NIT catalase assay CAT http://www.microbelibrary.org/library/laboratory-test/3226-catalase-test-protocol Carrine Blank catalase An enzymatic assay which tests for the presence of catalase in a microorganism. The catalase reaction converts hydrogen peroxide into water and oxygen: 2H2O2 -> 2 H2O + O2 For the catalase test a drop of 3% or 15% hydrogen peroxide is in contact with cells and the appearance of oxygen bubbles indicates a positive test result. No bubbles indicates a negative test result. There are several variants of the test: on a slide (where cells are smeared onto the glass), in a test tube where cells are placed into a hydrogen peroxide solution), an agar slant, or a colony on a plate. caseinase hydrolysis assay Carrine Blank Assays for the ability of an organism to break down the protein casein. Usually tested by incorporating casein into agar. A positive test will result in clearing of the agar due to degradation of the opaque casein. Commonly uses Milk Agar or Casein Agar. has component some casein casein hydrolysis digitonin inhibition assay Wikipedia:Digitonin Carrine Blank A chemcial sensitivity assay for the ability to grow in the presence of digitonin, a glycoside compound that solubilizes lipids and cell membranes. gelatinase assay liquifies gelatin Carrine Blank Wikipedia:Gelatinase gelatin hydrolysis gelatinase GEL An assay for the presence of gelatinase in a microorganism. Gelatinase is a proteolytic enzyme that breaks down gelatin (a partially hydrolyzed form of collagen derived from animal tissue that is comprised of peptides and proteins) via a hydrolysis reaction. Gelatin hydrolysis (a positive reaction) is found when the liquefaction of a gelatin-containing substrate is observed. phosphatase assay p-nitrophenyl phosphate p-nitrophenyl-phosphate indoxylphosphate An enzymatic assay which tests for the presence of phosphatases in a microorganism, using various chromogenic substrates. nitrophenylphosphate PO4 indoxyl phosphatase PHOS Carrine Blank phosphatase PHS resazurin reduction assay An assay for the ability of a microorganism to reduce resazurin. Resazurin is a dye in which under oxidizing conditions it is blue, however when reduced becomes pink (it is also strongly fluorescent). It functions as a redox indicator, and in viability tests. resazurin reduction resazurin RES Carrine Blank tellurite reduction assay In the tellurite reduction test, tellurite is reduced to metallic tellurium, which is dark grey. Some microorganisms are resistant to tellurite, and thus tellurite reduction in those organisms is an assay of redox (respiratory) metabolism. The extent of tellurite reduction is also a measure of the extent of respiratory metabolism. Tellurite added to plates, when reduced, generates colonies that are dark grey in color. Tellurite is toxic, and so may be inhibitory at concentrations ranging from 40-120 mg/L. Nagai S. 1965. Differential reduction of tellurite by growing colonies of normal yeast and respiration-deficient mutans. J Bact 90(1):220-222. Carrine Blank API 32 Staph API® 32 Staph; Identification system for staphylococci ; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. Carrine Blank suite of microbiological diagnostic tests A set of assays that are sold as diagnostic/identification kits that are purchased that can identify commonly isolated microorganisms. Carrine Blank API microbial identification test kit Wikipedia:Analytical_profile_index Test kits old by bioMerieux, Inc. (www.biomerieux.com/en) Carrine Blank API = analytical profile index, a system for the identification of microorganisms. API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. API ZYM Humble MW, King A, Phillips I. 1977. API ZYM: a simple rapid system for the detection of bacterial enzymes. J. Clin. Path. 30:275-277. API ZYM® – Semiquantification of enzymatic activities; http://www.biomerieux-usa.com; System for the research of enzymatic activity API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. Carrine Blank API 20E Carrine Blank API® 20E – 18-24 hour identification of Enterobacteriacae and other non-fastidious gram negative bacteria; http://www.biomerieux-usa.com API, and API 20E, are a registered trademark belonging to bioMerieux SA or one of its subsidiaries. API 50CH Carrine Blank API® 50 CH – Performance of carbohydrate metabolism tests; http://www.biomerieux-usa.com API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. API Rapid ID-32A Carrine Blank API® Rapid ID 32 A – 4-hour identification of anaerobes; http://www.biomerieux-usa.com; Identification system for anaerobes API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. API 20A API 20A® – 24-hour identification of anaerobes; http://www.biomerieux-usa.com; System for the identification of anaerobes API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. Carrine Blank API ID-32GN API® ID 32 GN; Automatic identification system for Gram-negative rods; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. Carrine Blank API 20NE Carrine Blank API 20NE® – 24 to 48-hour identification of Gram negative non-Enterobacteriaceae; http://www.biomerieux-usa.com API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. N-acetyl-beta-glucosaminidase assay with naphthol N-acetyl-b-glucosaminidase N-acetyl-b-D-glucosaminidase 1-naphthyl-N-acetyl-beta-D-glucosaminide Carrine Blank N-acetyl-beta-D-glucosaminidase 1-naphthyl-N-acetyl-b-D-glucosaminide beta-N-acetylglucosaminidase N-acetyl-beta-glucosaminidase bNAG An assay for the activity of N-acetyl-beta-glucosaminidase in a microorganism. Uses the substrate 1-naphthyl-N-acetyl-beta-D-glucosaminide at pH 5.4. N-acetyl-beta-glucosaminidase activity (catalyzing the hydrolysis of terminal beta-N-acetylglucosamine residues from oligosaccharides) will cleave the substrate, producing 1-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is brown in color. zzzz naphthol is not napthyl beta-mannosidase assay using BrNaphthol Carrine Blank 6-bromo-2-naphthyl-a-D-mannopyranoside A carbohydrate hydrolysis assay for the activity of beta-mannosidase in a microorganism using the substrate 6-bromo-2-naphthyl-beta-D-mannopyranoside at pH 5.4. beta-Mannosidase activity (catalyzing the hydrolysis of terminal non-reducing beta-D-mannose residues in beta-D-mannosides) will cleave the substrate, producing 6-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (a bromo substituted azo dye) that is purple in color. 6-Br-2-naphthyl-alpha-D-mannopyranoside 6-Br-2-naphthyl-a-D-mannopyranoside 6-bromo-2-naphthyl-alpha-D-mannopyranoside Wikipedia:Beta-mannosidase beta-glucosidase assay with BrNapthol 6-Br-2-naphthyl-b-D-glucopyranoside Carrine Blank 6-bromo-2-naphthyl-beta-D-glucopyranoside An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate 6-bromo-2-naphthyl-beta-D-glucopyranoside at pH 5.4. beta-Glucosidase activity will cleave the substrate, producing 6-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (a bromo subsituted azo dye) that is purple in color. 6-Br-2-naphthyl-beta-D-glucopyranoside 6-bromo-2-naphthyl-b-D-glucopyranoside beta-glucosidase assay with naphthol Carrine Blank 2-naphthyl-beta-D-glucopyranoside An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate 2-naphthyl-beta-D-glucopyranoside at pH 5.4. beta-Glucosidase activity will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is purple in color. 2-naphthyl-b-D-glucopyranoside beta-glucuronidase assay with naphthol AS-BI An assay for the activity of beta-glucuronidase in a microorganism. Uses the substrate 2-naphthol AS bis-beta-D-glucuronide (naphthol AS-BI beta-D-glucuronide, O-beta-D-glucuronosyl-naphtol AS-BI, naphthol ASBI-glucuronic acid) at pH 5.4. beta-Glucuronidase will cleave the substrate, producing 6-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (a bromo subsituted azo dye) that is blue in color. A positive test yields a blue color; a negative test is colorless, pale gray, or pale beige. Carrine Blank naphthol-AS-B1-b-D-glucuronic acid naphthol-AS-BI-b-D-glucuronide naphthol-AS-BI-beta-D-glucuronide bGUR naphthol-AS-B1-beta-D-glucuronic acid beta-galactosidase assay with naphthol 2-naphthyl-b-D-galactopyranoside Carrine Blank 2-naphthyl-beta-D-galactopyranoside An assay for the activity of beta-galactosidase in a microorganism. Uses the substrate 2-naphthyl-beta-D-galactopyranoside at pH 5.4. beta-Galactosidase will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is purple in color. bGAL beta-galactosidase assay with BrNaphthol 6-Br-2-naphthyl-b-D-galactopyranoside 6-bromo-2-naphthyl-beta-D-galactopyranoside BNGAL 6-Br-2-naphthyl-beta-D-galactopyranoside Carrine Blank An assay for the activity of beta-galactosidase in a microorganism. Uses the substrate 6-bromo-2-naphthyl-beta-D-galactopyranoside at pH 5.4. beta-Galactosidase will cleave the substrate, producing 6-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (a bromo subsituted azo dye) that is purple in color. 6-bromo-2-naphthyl-b-D-galactopyranoside phosphoamidase assay phosphohydrolase naphthol phosphohydrolase napthol-AS-BI-phosphohydrolase naphthol-phosphohydrolase naphthol-AS-BI-phosphodiamide naphthol-AS-BI-phosphate naphthol-AS-BIphosphohydrolase naphthol-AS-BI-phosphoamidase phosphoamidase naphthol-AS-B1-phosphodiamide naphthol-AS-BI-phosphohydrolase Uses the substrate naphthol AS BI-phosphate (aka naphthol AS bis-phosphodiamide, or 7-bromo-3-hydroxy-2-naphthoic-o-anisidide phosphate) at pH 5.4. Naphthol-AS-BI-phosphohydrolase will cleave the substrate, producing 7-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (a bromo subsituted azo dye) that is blue in color. A phosphoamidase enzyme is a hydrolase enzyme that acts on phosphorus-nitrogen bonds, releasing phosphate. For example: N-phosphocreatine + H2O <=> creatine + phosphate naphtol-AS-BI-phosphohydrolase Wikipedia:Phosphoamidase Carrine Blank acid phosphatase assay with naphthol 2-naphthyl-phosphate 2-naphthyl phosphate 2-naphthyl phosphate at pH 5.4 Carrine Blank Uses the substrate 2-naphthyl phosphate at pH 5.4. Acid phosphatase will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color. chymotrypsin assay An assay for the presence of chymotrypsin in a microorganism. When the chemical substrate of chymotrypsin is cleaved by the enzyme, a colored produce is released which is then measured using spectrophotometry. Wikipedia:Chymotrypsin a-chymotrypsin chymotrypsin Carrine Blank trypsin assay Carrine Blank trypsin Wikipedia:trypsin An assay for the presence of trypsin in a microorganism. When the chemical substrate of trypsin is cleaved by the enzyme, a colored produce is released which is then measured using spectrophotometry. L-cystine arylamidase assay Carrine Blank zzzz di-napthylamide is not napthylamide cystine aminopeptidase L-cystyl-2-naphthylamide L-cystine-2-naphthylamide L-cystyl-b-naphthylamide L-cystine-beta-naphthylamide An alpha-amino acid arylamidase assay that uses the substrate L-cystine-di-2-naphthylamide at pH 7.5. Cystine arylamidase activity (which could be from cystine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. The presence of thiols converts this compound to L-cysteine-2-naphthylamide. A positive reaction is orange; a negative reaction is colorless. L-cystine-b-naphthylamide cystine arylamidase L-cystyl-beta-naphthylamide L-valine arylamidase assay Carrine Blank valine aminopeptidase L-Valine AMC L-valine-2-naphthylamide L-valyl-b-naphthylamide An alpha-amino acid arylamidase assay that uses the substrate L-valyl-2-naphthylamide at pH 7.5. Valine arylamidase activity (which could be from valine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-valyl-2-naphthylamide valine arylamidase L-Valine 7-amido-4-methylcoumarin L-valyl-beta-naphthylamide L-leucine arylamidase assay L-leucyl-beta-naphthylamide An alpha-amino acid arylamidase assay that uses the substrate L-leucyl-2-naphthylamide (L-leucine-beta-naphthylamide) at pH 7.5. Leucine arylamidase activity (which could be from leucine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. leucine aminopeptidase leucine arylamidase Carrine Blank LeuA L-leucyl-b-naphthylamide lAP L-leucyl-2-naphthylamide LEU L-leucine-2-naphthylamide lipase C14 assay Uses the substrate 2-naphthyl myristate at pH 7.1. Esterases that can hydrolyze C14 compounds will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color. lipase C14 Wikipedia:Lipase 2-naphthyl myristate 2-naphthyl myristic acid Carrine Blank lipase C8 assay esterase lipase (C8) 2-naphthyl caprite ester lipase (C8) 2-naphthyl caprylate ester lipase C8 Uses the substrate 2-naphthyl caprylate at pH 7.1. Esterases that can hydrolyze C8 compounds will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color. lipase C8 Wikipedia:Lipase 2-naphthyl capric acid Carrine Blank esterase lipase alkaline phosphatase assay with naphthol Carrine Blank 2-naphthyl phosphate 2-naphthyl-phosphate Alkaline phosphatase assay that uses the substrate 2-naphthyl phosphate at pH 8.5. Alkaline phosphatase will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color. PAL esterase C4 assay esterase (C4) Test for C4 esterase in a microorganism. Uses the substrate 2-naphthyl butyrate at pH 7.1. Esterases that can hydrolyze C4 compounds will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color. esterase C4 Carrine Blank 2-naphthyl butyrate Wikipedia:Esterase beta-L-fucosidase assay with naphthol Carrine Blank 2-naphthyl-beta-L-fucoside 2-naphthyl-beta-L-fucopyranoside 2-naphthyl-b-L-fucopyranoside 2-naphthyl-b-L-fucoside An assay for the activity of beta-L-fucosidase in a microorganism. Uses the substrate 2-naphthyl-beta-L-fucoside at pH 5.4. beta-Fusosidase activity (catalyzing the hydrolysis of terminal non-reducing beta-L-fucose residues in beta-fucosides) will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is purple in color. alanyl phenylalanyl proline arylamidase assay APPA Carrine Blank L-alanyl-L-phenylalanyl-L-proline-beta-naphthylamide alanyl phenylalanyl proline aminopeptidase An assay for alanyl phenylalanyl proline arylamidase activity. Uses the substrate L-alanyl-L-phenylalanyl-L-proline-2-naphthylamide at pH 7.5. Alanyl phenylalanyl proline arylamidase activity (which could be from alanyl phenylalanyl proline aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-alanyl-L-phenylalanyl-L-proline-2-naphthylamide alanyl phenylalanyl proline arylamidase L-alanyl-L-phenylalanyl-L-proline-b-naphthylamide Ala-Phe-Pro arylamidase beta-galactosidase assay with ONPG o-nitrophenol-b-D-galactopyranoside o-nitrophenyl-beta-D-galactopyranoside o-NP-beta-D-galactopyranoside Carrine Blank o-NP-b-D-galactopyranoside ONPG PNPG o-nitrophenyl-b-galactoside o-nitrophenyl-b-D-galactopyranoside o-nitrophenyl-b-D-galactoside ONP-beta-D-galactopyranoside 2-nitrophenyl-beta-D-galactoside 2-nitrophenyl-beta-D-galactopyranoside 2-nitrophenyl-b-D-galactoside ONP-b-D-galactopyranoside An assay for the activity of beta-galactosidase in a microorganism. This assay measures beta-galactosidase activity using the colorless substrate ONPG (ortho-nitrophenyl-beta-D-galactoside, 2-nitrophenyl beta-D-galactopyranoside). beta-Galactosidase cleaves the substrate producing galactose and ortho-nitrophenol (yellow). A positive test yields a yellow color; a negative test is colorless. This test requires an additional inducer of transcription of the lac operon (such as IPTG; isopropyl beta-D-1-thiogalactopyranoside). Wikipedia:ortho-Nitrophenyl-β-galactoside 2-nitrophenyl-b-D-galactopyranoside bGAL arginine dihydrolase assay decarboxylation of the amino acid arginine by arginine dihydrolase arginine deiminase The purpose of this assay is to determine if a microbial isolate is capable of metabolizing arginine under anaerobic conditions. When arginine is metabolized, the pH of the medium increases due to the accumulation of ammonia and organic amines (e.g. putrescine). This turns color of the pH indicator (e.g. bromcresol purple, cresol red) to red/orange. A positive test yields a red/orange color; a negative test is yellow. Arginine dihydrolase (arginine deiminase) catalyzes the reaction: L-arginine + H2O <=> L-citrulline + NH3 Wikipedia:Arginine_deiminase Carrine Blank arginine dihydrolase ADH2s arginine dehydrolase arginine dihydroxylase ARG ADH arginine hydrolysis lysine decarboxylase assay lysine dihydrolase As assay to determine if a microbial isolate is capable of metabolizing lysine under anaerobic conditions. When lysine is metabolized, the pH of the medium increases due to the accumulation of ammonia and organic amines (e.g. cadaverine). This turns color of the pH indicator (e.g. bromcresol purple, cresol red) to red/orange. A positive test yields a red/orange color; a negative test is yellow. Lysine decarboxylase catalyzes the following reaction: L-lysine <=> cadaverine + CO2 Wikipedia:Lysine_decarboxylase LDC "Lysine dihydrolase" test results found in a small number of prokaryotic taxonomic descriptions is likely an error - it is more likely to be "lysine decarboxylase" test results. Carrine Blank lysine decarboxylase decarboxylation of the amino acid lysine by lysine decarboxylase (sic) ornithine decarboxylase assay ORN Carrine Blank L-orgnithine decarboxylase decarboxylations of the amino acid ornithine by ornithine decarboxylase (sic) ODC Wikipedia:Ornithine_decarboxylase ornithine carboxy-lyase The purpose of this test is to determine if a microbial isolate is capable of metabolizing ornithine under anaerobic conditions. When ornithine is metabolized, the pH of the medium increases due to the accumulation of ammonia and organic amines (e.g. putrescine). This turns color of the pH indicator (e.g. bromcresol purple, cresol red) to red/orange. A positive test yields a red/orange color; a negative test is yellow. Ornithine decarboxylase catalyzes the reaction: L-ornithine <=> putrescine + CO2 ornithine decarboxylase tryptophan deaminase assay L-tryptophan transaminase tryptophane deaminase tryptophan aminotransferase tryptophan deaminase Carrine Blank The purpose of this assay is to determine if a microbial isolate is capable of metabolizing tryptophan under anaerobic conditions, via the enzyme L-tryptophan aminotransferase (L-tryptophan transaminase). Tryptophan is deamined to form indolepyruvic acid (indole-3-pyruvate, an alpha keto acid) and ammonia. When FeCl3 (ferric chloride) is added, it reacts with the indolepyruvic acid with the presence of hydrazine compounds in the culture media to produce a brown/red precipitate. A positive test yields a brown/red color; a negative test is yellow. L-tryptophan aminotransferase catalyzes the following reaction: L-tryptophan + 2-oxoglutarate <-> indole-3-pyruvate + L-glutamate TDA gelatinase assay using charcoal beads gelatinase GEL API commercial test for the presence of gelatinase in a microorganism. Charcoal is added to gelatin beads. If gelatinase is present, the gelatin will be liquified and the charcoal released in to the solution. A positive result shows diffusion of black pigment (charcoal); a negative result shows no diffusion of charcoal, with intact gelatin beads. Carrine Blank liquifies gelatin organic molecular entity fermentation/oxidation assay Organic carbon metabolism assay where an organic molecular entity is tested to determine if it can be fermented or oxidized by a microorganism. The bottom of the reaction chamber becomes anaerobic where fermentation can occur, whereas the top of the chamber is in contact with oxygen and therefore is aerobic. Fermentation of a neutral carbohydrate substrate results in acidic condition, or a yellow color. A positive result is yellow; a negative result is blue or blue-green. --------------------------------------- Metabolism of an organic acid results in a transition from acidic to neutral pH. A positive result is green to blue; a negative result is yellow. Carrine Blank requires particular salts for growth Sodium, Potassium, Calcium, or Magnesium ions are required to be present in order for growth of a particular microorganism to occur. Carrine Blank D-glucose fermentation/oxidation assay GLU Carrine Blank Assays for the ability of a microorganism to ferment or oxidize D-glucose. D-mannitol fermentation/oxidation assay Carrine Blank MAN Assays for the ability of a microorganism to ferment or oxidize D-mannitol. inositol fermentation/oxidation assay INO Assays for the ability of a microorganism to ferment or oxidize inositol. Carrine Blank D-glucitol fermentation/oxidation assay SOR Carrine Blank Assays for the ability of a microorganism to ferment or oxidize D-sorbitol (D-glucitol). L-rhamnose fermentation/oxidation assay Carrine Blank Assays for the ability of a microorganism to ferment or oxidize L-rhamnose. RHA sucrose fermentation/oxidation assay Carrine Blank SAC Assays for the ability of a microorganism to ferment or oxidize sucrose. beta-melibiose fermentation/oxidation assay Carrine Blank Assays for the ability of a microorganism to ferment or oxidize D-melibiose (beta-melibiose). MEL amygdalin fermentation/oxidation assay AMY Assays for the ability of a microorganism to ferment or oxidize amygdalin. Carrine Blank L-arabinose fermentation/oxidation assay ARA Carrine Blank Assays for the ability of a microorganism to ferment or oxidize L-arabinose. triphenyl tetrazolium reduction assay TTZ TTC tetrazolium reduction A test using triphenyl tetrazolium chloride (2,3,5-triphenyl-2H-tetrazolium chloride, tetrazolium chloride, TTC) is a redox indicator used to quantify respiration. Actively respiring cells will reduce TTC (clear/white) to insoluble TPF (1,3,5-triphenylformazan) which is a red color. A positive test result is pink red (or results in a deposit in the base of the cupule); a negative test result is colorless to pale pink. tetrazolium chloride Carrine Blank aerobic triphenyl tetrazolium reduction assay Assays the ability of a microorganism to reduce triphenyl tetrazolium chloride under aerobic conditions (using aerobic respiration). The color change (production of the red reduced product will occur near the top of the cupule where oxygen is present. A positive test result is pink red (or results in a deposit in the base of the cupule); a negative test result is colorless to pale pink. Carrine Blank aerobic triphenyl tetrazolium reduction anaerobic triphenyl tetrazolium reduction assay Carrine Blank Assays the ability of a microorganism to reduce triphenyl tetrazolium chloride under anaerobic conditions (using anaerobic respiration). The color change (production of the red reduced product will occur near the bottom of the cupule where oxygen is absent. A positive test result is pink red (or results in a deposit in the base of the cupule); a negative test result is colorless to pale pink. anaerobic triphenyl tetrazolium reduction lipase assay Carrine Blank LLIP lipolytic activity lipase A hydrolase assay for the presence of lipase activity in a microorganism. Lipase enzymes split longer-chained esters into an acid and an alcohol via a hydrolysis reaction. lipase activity fatty acid ester lipolysis LIP hydrogen sulfide assay sulfide production H2S production H2S lead acetate is blackened lead acetate An assay for the production of hydrogen sulfide in a microorganism from sodium thiosulfate under anaerobic conditions. After incubation, FeCl3 (ferric chloride) is added. If H2S is formed, it will react with the ferric chloride to form a black/brown ferrous sulfide precipitate. A positive test results in a black deposit (or thin line); a negative test results in a colorless or greyish color. Can also be performed using a strip of filter paper soaked in saturated lead acetate and dried. When suspended above the medium in the presence of hydrogen sulfide the paper will turn black. hydrogen sulfide production Carrine Blank sulfur reduction test organic molecular entity fermentation assay methyl red test acidification fermentable sugars fermentation phenol red decolorized ferment phenol red test fermentative metabolism methyl red fermentable carbohydrate cultures decolorizes the sulphonphthalein dyes brom cresol purple and phenol red fermentable carbohydrate Carrine Blank acid production Organic carbon metabolism assay where an organic molecular entity is tested to determine if it can be fermented by a microorganism. For fermentation of substrates in the presence of BROMCRESOL PURPLE (bromocresol purple), acidification of the medium results in a color change of the indicator to yellow or yellow-green. Absence of fermentation results in a neutral pH, or purple color. A positive reaction is purple; a negative reaction is yellow or yellow-green. ---------------------------------- For fermentation of substrates in the presence of PHENOL RED acidification of the medium results in a color change of the indicator to yellow. Absence of fermentation results in a neutral pH, or red color. A positive reaction is yellow; a negative reaction is red. ----------------------------------- For fermentation of substrates in the presence of BROMOTHYMOL BLUE, acidification of the medium results in a color change of the indicator to yellow or green. Absence of fermentation results in a neutral pH, or blue color. A positive reaction is green to yellow; a negative reaction is blue. ---------------------------------- fermentable substrates bromcresol purple decolorized fermentation acids aerobic acid production D-glucose fermentation assay D-glucose acidification dextrose dGLU Assays for the ability of a microorganism to ferment D-glucose. Carrine Blank Dglucose D-glucose fermentation OFF D-glucose GLU D-mannitol fermentation assay MAN D-mannitol acidification D-mannitol Dmanniol D-mannitiol Dmannitol MNL dMAN D-mannitol fermentation Carrine Blank dulcite Assays for the ability of a microorganism to ferment D-mannitol. beta-lactose fermentation assay D-lactose acidification Carrine Blank D-lactose fermentation LAC D-lactose Assays for the ability of a microorganism to ferment beta-lactose. sucrose fermentation assay SUC saccharose sucrose acidification Dsucrose D-saccharose fermentation SAC Carrine Blank Assays for the ability of a microorganism to ferment sucrose. D-saccharose acidification sucrose D-saccharose D-sucrose sucrose fermentation beta-maltose fermentation assay Assays for the ability of a microorganism to ferment beta-maltose. Carrine Blank beta-maltose D-maltose acidification dMAL D-maltose fermentation D-maltose D-malose MAL salicin fermentation assay saticin salicine Dsalicin sugar aldehyde salicin acidification KSF L-salicin D-salicin SAL Assays for the ability of a microorganism to ferment salicin. salicin salicin fermentation Carrine Blank sulicin D-xylose fermentation assay D-xylose acidification dXYL Assays for the ability of a microorganism to ferment D-xylose. D-xyloze XYL Dxylose Carrine Blank D-xylose fermentation D-xylose L-arabinose fermentation assay L-arabinose lARA ARA L-arabinose fermentation Carrine Blank Larabinose L-arabinose acidification L-arabinol Assays for the ability of a microorganism to ferment L-arabinose. glycerol fermentation assay glycerol fermentation glycerol acidification Carrine Blank glucerol glycerol glycerin GLY gkycerol Assays for the ability of a microorganism to ferment glycerol. beta-cellobiose fermentation assay beta-cellobiose Dcellobiose Carrine Blank Assays for the ability of a microorganism to ferment beta-cellobiose. dCEL D-celiobiose D-cellobiose acidification D-cellobiose fermentation D-cellobiose CEL Dcellubiose D-mannose fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment D-mannose. D-mennose Dmannose D-mannose D-mannose acidification dMNE MNE D-mannose fermentation melezitose fermentation assay MLZ dMLZ D-melezitose fermentation melezitose acidification a-melezitose Carrine Blank Dmelezitose melezitose fermentation meleziose melezitose melezitol melizitol Assays for the ability of a microorganism to ferment melezitose. D-melezitose melizitose D-melezitose acidification raffinose fermentation assay raffinose acidification D-raffinose fermentation alpha-raffinose D-raffinose Assays for the ability of a microorganism to ferment D-raffinose (raffinose). dRAF beta-raffinose D-rafnose raffinose D-melitose raflinose rafnose Carrine Blank L-raffinose Draffinose D-raffinose acidification RAF raffinose fermentation D-glucitol fermentation assay D-sorbitol acidification D-dorbitol Carrine Blank D-sorbitol Assays for the ability of a microorganism to ferment D-glucitol. SOR dSOR Dsorbiol D-sorbitol fermentation Dsorbitol L-rhamnose fermentation assay RHA lRHA L-rhamnose fermentation Lrhamnose Carrine Blank L-rhamnose Assays for the ability of a microorganism to ferment L-rhamnose. L-rhamnose acidification alpha,alpha-trehalose fermentation assay D-trehalose D-trehalose acidification Assays for the ability of a microorganism to ferment alpha,alpha-trehalose. dTRE Carrine Blank Dtrehalose TRE alpha,alpha-trehalose D-trehalose fermentation D-tehalose esculin ferric citrate assay esculin hydrolysis test aesculin ferric citrate aesculin ferric citrate test An assay for the hydrolysis of esculin in a microorganism. Esculin is a coumarin glycoside. Many species of microorganisms can hydrolyze esculin into glucose and aesculetin (6,7-dihydroxycoumerin). In the presence of ferric iron, aesculetin reacts to form a dark brown to black phenolic iron compound. A positive result generates a dark brown/black precipitate; a negative result generates no precipitate (yellow color). Because esculin is fluorescent, under uv illumination, a positive result generates no fluorescence; a negative result generates fluorescence. Ferric iron may be reduced to hydrogen sulfide (resulting in a dark brown to black precipitate), resulting in a false positive reaction. Sulfide will be produced only under anaerobic conditions, so the precipitate will only be seen at the bottom of the cupule, away from oxygen. Confirmation of the test results should therefore be made using uv illumination. Carrine Blank esculin hydrolyse aesculin hydrolase esculin hydrolase esculin hydrolysis esculin ferric citrate aesculin hydrolysis ESC esculin ferric citrate test erythritol fermentation assay erythrol ERY erythrotol erythitol mesoerythritol DL-erythritol Carrine Blank erythritol fermentation Assays for the ability of a microorganism to ferment erythritol. erythritol erythritol acidification beta-galactosidase assay with PNPG PNPGAL 4-nitrophenyl-b-D-galactopyranoside para-nitrophenyl-b-D-galactopyranoside p-nitrophenyl-b-D-galactoside p-NP-b-D-galactopyranoside 4-nitrophenyl-beta-D-galactopyranoside p-NP-beta-D-galactopyranoside p-nitrophenyl galactoside p-nitrophenyl-beta-D-galactopyranoside bGAL p-nitrophenyl-b-D-galactopyranoside Carrine Blank An assay for the activity of beta-galactosidase in a microorganism. An assay for beta-galactosidase activity using para-nitrophenyl-beta-D-galactopyranoside; 4-nitrophenyl-beta-D-galactopyranoside; PNPG). Beta-galactosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. PNPG has been found in some strains to have a higher hydrolysis rate than ONPG, and beta-galactosidase has a higher substrate preference for PNPG. Lee E-G, Kim S, Oh D-B, Lee SY & Kwon O. 2012. Distinct roles of beta-galactosidase paralogues of the rumen bacterium Mannheimia succiniciproducens. J Bacterol 194(2):426-436. para-nitrophenyl-beta-D-galactopyranoside PNPG D-glucose assimilation assay D-glucose assimilation Carrine Blank Assays for the ability of a microorganism to assimilate D-glucose as a sole source of carbon and energy. dextrose D-glucose GLU dGLUa Dglucose dGLU L-arabinose assimilation assay L-arabinol Assays for the ability of a microorganism to assimilate L-arabinose as a sole source of carbon and energy. lARAa L-arabinose L-arabinose assimilation Carrine Blank ARA Larabinose D-mannose assimilation assay D-mannose MNE dMNEa Assays for the ability of a microorganism to assimilate D-mannose as a sole source of carbon and energy. D-mennose Dmannose Carrine Blank D-mannose assimilation D-mannitol assimilation assay Assays for the ability of a microorganism to assimilate D-mannitol as a sole source of carbon and energy. D-mannitol dulcite D-mannitiol Dmanniol D-mannitol assimilation MAN Carrine Blank Dmannitol N-acetyl-beta-D-glucosamine assimilation assay N-acetyl-betaglucosanme N-acetyl-beta-glucosamine N-acetyl-beta-D-glucosamine assimilation Carrine Blank N-acetyl-beta-D-glucosamine Assays for the ability of a microorganism to assimilate N-acetyl-beta-D-glucosamine as a sole source of carbon and energy. beta-maltose assimilation assay dMALa D-maltose assimilation D-maltose Assays for the ability of a microorganism to assimilate beta-maltose as a sole source of carbon and energy. beta-maltose assimilation Carrine Blank beta-maltose D-malose MAL D-gluconic acid assimilation assay Carrine Blank D-gluconate Assays for the ability of a microorganism to assimilate D-gluconate as a sole source of carbon and energy. D-gluconic acid assimilation dGNTa D-gluconate assimilation D-gluconic acid decanoic acid assimilation assay Assays for the ability of a microorganism to assimilate decanoate (decanoic acid) as a sole source of carbon and energy. capric acid assimilation decanoate decanoate assimilation Carrine Blank CAP n-capric acid caprate capric acid decanoic acid caprate assimilation adipic acid assimilation assay adipate assimilation ADI Carrine Blank adipinate Assays for the ability of a microorganism to assimilate adipate (adipic acid) as a sole source of carbon and energy. adipic acid adipic acid assimilation adipate (S)-malic acid assimilation assay sodium L-malate Carrine Blank lMLTa L-malic acid L-malate assimilation (S)-malate Assays for the ability of a microorganism to assimilate (S)-malate(2-) as a sole source of carbon and energy. L-malate citric acid assimilation assay fe citrate citric acid citrate (sodium) assimilation citrate sodium citrate trisodium citrate citric acid assimilation citrate utilization Carrine Blank ferric citrate CIT citrate assimilation titanium citrate trisodium citrate assimilation iron citrate CITa Assays for the ability of a microorganism to assimilate citrate (citric acid) as a sole source of carbon and energy. sodium citrate assimilation phenylacetic acid assimilation assay phenylacetate assimilation phenylacetic acid assimilation phenylacetic acid PAC phenyl-acetate o-toluic acid Carrine Blank phenyl acetic acid o-toluate phenyl acetate Assays for the ability of a microorganism to assimilate phenylacetate (phenylacetic acid) as a sole source of carbon and energy. phenylacetate organic molecular entity assimilation assay Organic carbon metabolism assay where an organic molecular entity is tested to determine if it can be assimilated or oxidized by a microorganism, using it both as a carbon source and as a source of energy. Assimilation of the substrate in the presence of a single organic carbon compound results in turbidity due to growth, under oxic conditions. Oxidation means that the compound is oxidized (which could be followed by the oxidation of a terminal electron acceptor dye, such as resazurin or tetrazolium-derivative). Note: this definition does not include fermentation. A positive result is the presence of turbidity (opaque); a negative result is transparent. Or a positive result is the presence of a change in color of a redox dye; a negative result is no change in color. Carrine Blank D-arabinose fermenation assay D-arabinose Carrine Blank DARA D-arabinose fermentation Darabinose D-arabinose acidification Assays for the ability of a microorganism to ferment D-arabinose. D-ribose fermentation assay D-ribose dRIB2 Carrine Blank D-ribose fermentation Dribose RIB D-ribose acidification dRIB Assays for the ability of a microorganism to ferment D-ribose. L-xylose fermentation assay L-xylose fermentation Assays for the ability of a microorganism to ferment L-xylose. L-xylose acidification LXYL Lxylose Carrine Blank L-xylose D-ribitol fermentation assay D-ribitol D-adonito D-adonitol acidification Dadonitol D-ribitol acidification ADO Carrine Blank Assays for the ability of a microorganism to ferment D-ribitol. D-adonitol fermentation D-ribitol fermentation D-adonitol methyl beta-D-xylopyranoside fermentation assay methyl-D-xylopyranoside methyl beta-D-xyloside fermentation MDX Assays for the ability of a microorganism to ferment methyl-beta-D-xylopyranoside. methyl-D-xyloside methyl-beta-D-xylose methyl-beta-xylopyranoside beta-methyl-D-xylose methyl-beta-D-xylopyranside methyl beta-D-xylopyranoside fermentation Carrine Blank D-methyl-beta-D-xylopyranoside methyl-beta-D-xyloside methyl beta-D-xylopyranoside methyl beta-D-xylopyranoside acidification methyl-beta-Dxylopyranoside beta-methyl-D-xyloside methyl-beta-Dxyloside methyl-D-xyloside fermentation methyl-beta-D-xylopyranoside MdX methyl beta-D-xyloside D-galactose fermentation assay GAL D-galatose Dgalacose D-galactose galactose fermenation D-galactose fermentation galactose acidification Dsalactose Carrine Blank D-galactose acidification galactose Assays for the ability of a microorganism to ferment D-galactose. Dgalactose dGAL D-fructose fermenation assay Carrine Blank D-fructose acidification Assays for the ability of a microorganism to ferment D-fructose. D-frucose Dfructose FRU Dfrucose D-levulose D-fructose D-fructose fermentation L-sorbose fermentation assay L-sorbose acidification Carrine Blank L-sorbose Assays for the ability of a microorganism to ferment L-sorbose. SBE L-sorbose fermentation Lsorbose galactitol fermentation assay galactitol dulcitol acidification dulcitol fermentation Assays for the ability of a microorganism to ferment galactitol. dulcital dulcit dulcitole dulicitol DUL D-dulcitol dulcitol dulciol meso-dulcitol Carrine Blank inositol fermentation assay inositol inosin inositol acidification Carrine Blank inositol fermentation inosiol INO Assays for the ability of a microorganism to ferment inositol. inosite methyl alpha-D-mannoside fermentation assay methyl-a-D-mannose methy a-D-mannopyanoside methyl-a-D-mannotpyranside methyl-aD-mannopyranoside methyl-a-Dmannoside methyl-D-mannoside a-methyl-D-mannose glycosides a-methyl-D-mannose methyl-D-mannopyranoside Carrine Blank methyl-a-Dmannopyrnaoside methyl-alpha-D-mannoside a-methyl-D-mannoside methyl alpha-D-mannopyranoside acidification methyl-a-D-mannoside methyl a-D-mannopyranoside methyl alpha-D-mannopyranoside a-methyl mannoside a-methylmannoside methyl a-D-mannoside assimilation methyl alpha-D-mannopyranoside fermentation MDM methyl a-D-mannoside acidification methyl-a-mannoside methyl alpha-D-mannoside Assays for the ability of a microorganism to ferment methyl-alpha-D-mannoside. methyl alpha-D-glucopyranoside fermentation assay a-methyl-glucoside methyl-a-D-glucoside methyl-aD-glucopyranoside methyl alpha-D-glucopyranoside fermentation MDG Assays for the ability of a microorganism to ferment methyl-alpha-D-glucopyranoside. MdG methyl-a-D-glucose methyl alpha-D-glucopyranoside acidification a-methyl-D-glycoside methyl-a-Dglucopyranoside methyl-alpha-D-glucoside alpha-methyl glucoside methyl-a-Dglucoside a-methylglucoside Carrine Blank methyl-a-D-glucopyranoside acidification methyl alpha-D-glucopyranoside methyl-a-D-glycoside methyl-a-D-glucopyranosdie methyl a-D-glucopyranoside methyl-a-D-glucopyranoside methyl-a-glucoside a-methyl glucoside a-methyl-D-glucoside N-acetyl-beta-D-glucosamine fermentation assay Carrine Blank N-acetyl-b-glucosamine N-acetyl-beta-D-glucosamine acidification N-acetyl-beta-D-glucosamine fermentation N-acetyl-beta-D-glucosamine N-acetyl-betaglucosamine Assays for the ability of a microorganism to ferment N-acetyl-beta-D-glucosamine. D-amygdalin fermentation assay D-amygdalin Carrine Blank AMY D-amygdalin acidification D-amygdalin fermentation Assays for the ability of a microorganism to ferment D-amygdalin. hydroquinone O-beta-D-glucopyranoside fermentation assay arubutin Carrine Blank arbutine fermentation L-arbutin arbutine acidification arbutin arbutin acidification ARB arbutine arubtin rirbutin arbutin fermentation Assays for the ability of a microorganism to ferment hydroquinone O-beta-D-glucopyranoside. D-arbutin p-arbutin esculin fermentation assay Assays for the ability of a microorganism to ferment esculin. ESC esculin ferric citrate aesculin ferric citrate aesculin acidification aesculin fermentation esculin acidification esculin fermentation Carrine Blank aesculin esculin esculine beta-melibiose fermentation assay b-melibiose a-D-melibiose D-melibiose Carrine Blank a-melibiose D-melibiose fermentation beta-melibiose D-melibiose acidfication D-meliboise Assays for the ability of a microorganism to ferment D-melibiose (beta-melibiose). inulobiose fermentation assay inulose BMC Plant Biol. 2009; 9: 14. Published online Jan 28, 2009. doi: 10.1186/1471-2229-9-14 PMCID: PMC2660907 Construction of 12 EST libraries and characterization of a 12,226 EST dataset for chicory (Cichorium intybus) root, leaves and nodules in the context of carbohydrate metabolism investigation Nicolas Dauchot, Dominique Mingeot, Bénédicte Purnelle, Céline Muys, Bernard Watillon, Marc Boutry, and Pierre Van Cutsem. INU Carrine Blank inulose acidification inulin Assays for the ability of a microorganism to ferment inulose (inulobiose). Inulin is a fructooligosaccharide polymer, found in some plants, which often has a terminal glucose. Glucose-free inulin is inulose. Think it is more likely that it is a mistaken representation for inulobiose. inulose fermentation starch fermentation assay AMD Assays for the ability of a microorganism to ferment starch. starch acidification starch fermentation Carrine Blank starch sarch potato starch amidon glycogen fermentation assay glycogene fermentation Assays for the ability of a microorganism to ferment glycogen. glycogene glycogen glycogen fermentation D-glycogen glycogen acidification glycogene acidification Carrine Blank GLYG Dglycogen xylitol fermentation assay Assays for the ability of a microorganism to ferment xylitol. xylitol Carrine Blank xylitol acidification xylitol fermentation D-xylitol XLT gentiobiose fermentation assay GEN gentiobiose acidification b-gentibiose gentiobiose Carrine Blank gentiobiose fermentation gentobiose b-gentobiose genitobiose beta-gentibiose b-gentiobiose Assays for the ability of a microorganism to ferment gentiobiose. beta-gentiobiose gentibiose beta-gentobiose turanose fermentation assay D-turanose Carrine Blank D-turanose fermentation D-turanose acidification turanose Dturanose TUR turanose fermentation D-tutanose turanose acidification Assays for the ability of a microorganism to ferment D-turanose. D-lyxose fermentation assay Carrine Blank D-lyxose acidification Dlyxose Assays for the ability of a microorganism to ferment D-lyxose. LYX D-iyxose D-lyxose fermentation D-lyxose D-tagatose fermentation assay Assays for the ability of a microorganism to ferment D-tagatose. D-tagatose acidification Dtagatose dTAG TAG D-tagatose Carrine Blank D-tagatose fermentation D-fucose fermentation assay D-fucose acidification Assays for the ability of a microorganism to ferment D-fucose. D-fucose fermentation DFUC D-fucose Dfucose Carrine Blank L-fucose fermentation assay LFUC L-fucose acidification L-fucose L-fucose fermentation Lfucose Assays for the ability of a microorganism to ferment L-fucose. Carrine Blank D-arabinitol fermentation assay D-arabinitol fermentation D-arabinitol Assays for the ability of a microorganism to ferment D-arabinitol. D-arabitol fermentation Carrine Blank D-arabinitol acidification D-arabitol DARL D-arabitol acidification L-arabinitol fermentation assay L-arabinitol Assays for the ability of a microorganism to ferment L-arabinitol. L-arabitol acidification LARL Carrine Blank L-arabinitol fermentation L-arabitol L-arabitol fermentation L-arabinitol acidification gluconic acid fermentation assay gluconate acidification Assays for the ability of a microorganism to ferment gluconate (gluconic acid). Carrine Blank GNT sodium gluconate gluconate fermentation gluconate potassium gluconate gluconic acid gluconic acid sodium salt 2-dehydro-D-gluconic acid fermentation assay 2-ketogluconate 2-keto-gluconate 2-dehydro-D-gluconic acid 2-ketogluconate acidification 2KG 2-cetogluconate Carrine Blank potassium 2-ketogluonate 2-ketogluconate fermentation Assays for the ability of a microorganism to ferment 2-ketogluconate (2-dehydro-D-gluconate; 2-dehydro-D-gluconic acid). potassium 2-ketogluconate potassium 2-cetogluconate 2-keto gluconate 2-dehydro-D-gluconate 2-keto-D-gluconate 5-dehydro-D-gluconic acid fermentation assay potassium 5-ketogluconat Assays for the ability of a microorganism to ferment 5-dehydro-D-gluconate (5-dehydro-D-gluconic acid). 5-ketogluconate Carrine Blank 5-dehydro-D-gluconate 5-ketogluconate fermentation 5-keto-D-gluconate potassium 5-ketogluconate 5-keto-Dgluconate 5-dehydro-D-gluconic acid 5KG 5-ketogluconate acidification potassium-5-ketogluconate potassium-5-cetogluconate 5-keto-gluconate 2-dehydro-D-gluconic acid assimilation assay 2-ketogluconate Carrine Blank 2-dehydro-D-gluconate 2-keto-D-gluconate assimilation potassium 2-ketogluconate 2-keto-gluconate 2-dehydro-D-gluconic acid potassium 2-ketogluonate 2-keto-gluconate assimilation 2KGa Assays for the ability of a microorganism to assimilate 2-dehydro-D-gluconate (2-dehydro-D-gluconic acid) as a sole source of carbon and energy. 2-keto gluconate 2-ketogluconate assimilation potassium 2-cetogluconate 2KGa 2KG 2-cetogluconate 2-keto-D-gluconate 3-hydroxybutyric acid assimilation assay b-hydroxy butyric acid hydroxy-b-DL-butyric aicd 3-hydroxybutyrate assimilation DL-3-hydroxybutyrate 3-hydroxybutyric acid 3OBU b-hydroxybutyrate 3-hydroxybutyric acid assimilation Assays for the ability of a microorganism to assimilate 3-hydroxybutyrate (3-hydroxybutyric acid) as a sole source of carbon and energy. zzzz check that carnitine is a synonym beta-hydroxybutyric acid DL-b-hydroxybutyrate 3-hydroxybutyrate b-hydroxy-L-butyric acid beta-hydroxybutyric acid assimilation Carrine Blank beta-hydroxybutyrate assimilation beta-hydroxybutyrate b-hydroxybutyric acid DL-carnitine 5-dehydro-D-gluconic acid assimilation assay 5-ketogluconic acid assimilation 5-ketogluconic acid 5-ketogluconate assimilation potassium 5-ketogluconat Assays for the ability of a microorganism to assimilate 5-dehydro-D-gluconate (5-dehydro-D-gluconic acid) as a sole source of carbon and energy. potassium 5-ketogluconate 5-dehydro-D-gluconic acid potassium-5-cetogluconate 5-ketogluconate 5KG 5-keto-gluconate Carrine Blank 5-keto-Dgluconate 5-dehydro-D-gluconate assimilation potassium-5-ketogluconate 5-keto-D-gluconate acetic acid assimilation assay acetate assimilation ACE acetic acid ACEa potassium acetate Assays for the ability of a microorganism to assimilate acetate (acetic acid) as a sole source of carbon and energy. sodium acetate actate acetic acetate acetic acid assimilation Carrine Blank L-alanine assimilation assay Carrine Blank L-alanine assimilation L-alanine Assays for the ability of a microorganism to assimilate L-alanine as a sole source of carbon and energy. L-fucose assimilation assay L-fucose Carrine Blank FUC L-fucose assimilation Assays for the ability of a microorganism to assimilate L-fucose as a sole source of carbon and energy. glycogen assimilation assay glycogen Dglycogen glycogen assimilation glycogene Carrine Blank GLYG glycogene assimilation Assays for the ability of a microorganism to assimilate glycogen as a sole source of carbon and energy. D-glycogen L-histidine assimilation assay Carrine Blank HIS lHISa Assays for the ability of a microorganism to assimilate L-histidine as a sole source of carbon and energy. L-histidine assimilation L-histidine inositol assimilation assay INO inosite inositol inositol assimilation Assays for the ability of a microorganism to assimilate inositol as a sole source of carbon and energy. inosin inosiol Carrine Blank itaconic acid assimilation assay Assays for the ability of a microorganism to assimilate itaconate (itaconic acid) as a sole source of carbon and energy. itaconic acid itaconate ITA itaconic acid assimilation itaconate assimilation Carrine Blank (S)-lactic acid assimilation assay i-lactic acid sodium L-lactate (S)-lactate L-lactate assimilation L-lactate Assays for the ability of a microorganism to assimilate (S)-lactate as a sole source of carbon and energy. L-lactic acids L-lactate isomer Llactic acid L-lactic acid (S)-lactic acid L-lactic acid assimilation lLATa Carrine Blank beta-melibiose assimilation assay dMELa b-melibiose Assays for the ability of a microorganism to assimilate beta-melibiose as a sole source of carbon and energy. beta-melibiose Carrine Blank MEL D-melibiose a-D-melibiose D-meliboise a-melibiose D-melibiose assimilation malonic acid assimilation assay malonate MNT malonic acid Assays for the ability of a microorganism to assimilate malonate (malonic acid) as a sole source of carbon and energy. malonate utilization Carrine Blank malonic acid assimilation sodium malonate malonate assimilation 3-hydroxybenzoic acid assimilation assay 3-hydroxybenzoic acid assimilation m-hydroxybenzoate Carrine Blank Assays for the ability of a microorganism to assimilate 3-hydroxybenzoate (3-hydroxybenzoic acid) as a sole source of carbon and energy. 3-hydroxybenzoic acid mOBE 3-hydroxybenzoate 3-hydroxybenzoate assimilation 4-hydroxybenzoic acid assimilation assay p-hydroxybenzoate 4-hydroxybenzoate pOBE Assays for the ability of a microorganism to assimilate 4-hydroxybenzoate (4-hydroxybenzoic acid) as a sole source of carbon and energy. 4-hydroxybenzoic acid 4-hydroxybenzoic acid assimilation 4-hydroxybenzoate assimilation Carrine Blank L-proline assimilation assay PRO Assays for the ability of a microorganism to assimilate L-proline as a sole source of carbon and energy. L-proline L-proline assimilation Carrine Blank lPROa propionic acid assimilation assay propionate assimilation PROP propionic acid assimilation Assays for the ability of a microorganism to assimilate propionate (propionic acid) as a sole source of carbon and energy. Carrine Blank propionic acid propionic sodium propionate propionate L-rhamnose assimilation assay Assays for the ability of a microorganism to assimilate L-rhamnose as a sole source of carbon and energy. lRHAa L-rhamnose assimilation Lrhamnose Carrine Blank L-rhamnose RHA D-ribose assimilation assay D-ribose RIB Dribose D-ribose assimilation Assays for the ability of a microorganism to assimilate D-ribose as a sole source of carbon and energy. Carrine Blank sucrose assimilation assay Dsucrose SAC SACa saccharose Assays for the ability of a microorganism to assimilate sucrose as a sole source of carbon and energy. D-saccharose assimilation D-sucrose saccharose assimilation sucrose assimilation sucrose Carrine Blank D-saccharose salicin assimilation assay L-salicin salicin saticin Assays for the ability of a microorganism to assimilate salicin as a sole source of carbon and energy. Dsalicin Carrine Blank salicin assimilation sulicin D-salicin assimilation salicine SAL D-salicin L-serine assimilation assay SER Carrine Blank Assays for the ability of a microorganism to assimilate L-serine as a sole source of carbon and energy. L-serine assimilation L-serine Lserine D-glucitol assimilation assay D-glucitol assimilation dSORa D-sorbitol assimilation Dsorbitol SOR Carrine Blank Assays for the ability of a microorganism to assimilate D-glucitol as a sole source of carbon and energy. D-glucitol Dsorbiol D-dorbitol D-sorbitol suberic acid assimilation assay Carrine Blank SUB Assays for the ability of a microorganism to assimilate suberate (suberic acid) as a sole source of carbon and energy. suberate suberic acid assimilation suberate assimilation suberic acid valeric acid assimilation assay valeric acid n-valeric acid valerate assimilation vaerate n-valerate valerate VALT Assays for the ability of a microorganism to assimilate valerate (valeric acid) as a sole source of carbon and energy. valeric acid assimilation Carrine Blank pentanoate enzymatic assay Carrine Blank An assay for the presence of an enzyme or enzymatic capability of a microorganism. alpha-galactosidase assay with pNP p-nitrophenyl-a-D-galactopyranoside 4-nitrophenyl-alpha-D-galactopyranoside An assay for the activity of alpha-galactosidase in a microorganism. Uses the substrate 4-nitrophenyl-alpha-D-galactopyranoside. Alpha-galactosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. p-NP-a-D-galactopyranoside p-nitrophenyl-alpha-D-galactopyranoside 4-nitrophenyl-a-D-galactopyranoside p-NP-alpha-D-galactopyranoside aGAL GAL para-nitrophenyl-a-D-galactopyranoside para-nitrophenyl-alpha-D-galactopyranoside Carrine Blank 6-phospho-beta-galactosidase assay with pNP para-nitrophenyl-beta-D-galactopyranoside-6-phosphate Carrine Blank p-NP-beta-D-galactopyranoside-6-phosphate p-NP-b-D-galactopyranoside-6-phosphate para-nitrophenyl-b-D-galactopyranoside-6-phosphate bGP 4-nitrophenyl-beta-D-galactopyranoside-6-phosphate An assay for the activity of 6-phospho-beta-galactosidase in a microorganism. Uses the substrate 4-nitrophenyl beta-D-galactopyranoside-6-phosphate. 6-phospho-beta-galactosidase (beta-galactosidase 6-phosphate) will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. p-nitrophenyl-beta-D-galactopyranoside-6-phosphate p-nitrophenyl-b-D-galactopyranoside-6-phosphate 4-nitrophenyl-b-D-galactopyranoside-6-phosphate alpha-glucosidase assay with pNP p-nitrophenyl-a-D-glucopyranoside para-nitrophenyl-alpha-D-glucopyranoside p-NP-a-D-glucopyranoside para-nitrophenyl-a-D-glucopyranoside 4-nitrophenyl-alpha-D-glucopyranoside An assay for the activity of alpha-glucosidase in a microorganism. Uses the substrate 4-nitrophenyl-alpha-D-glucopyranoside. Alpha-glucosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. GLU p-NP-alpha-D-glucopyranoside Carrine Blank aGLU p-nitrophenyl-alpha-D-glucopyranoside 4-nitrophenyl-a-D-glucopyranoside beta-glucosidase assay with pNP p-nitrophenyl-beta-D-glucopyranoside bGLU An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate 4-nitrophenyl-beta-D-glucopyranoside. Beta-glucosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. Carrine Blank p-nitrophenyl-b-D-glucopyranoside 4-nitrophenyl-beta-D-glucopyranoside p-NP-b-D-glucopyranoside p-NP-beta-D-glucopyranoside para-nitrophenyl-beta-D-glucopyranoside 4-nitrophenyl-b-D-glucopyranoside para-nitrophenyl-b-D-glucopyranoside sheath having distinctive features Morphologically differentiated filament part, where the sheath has distinctive features. Carrine Blank beta-glucuronidase assay with pNP p-nitrophenyl-beta-D-glucuronide p-NP-beta-D-glucuronide 4-nitrophenyl-b-D-glucuronide para-nitrophenyl-beta-D-glucuronide para-nitrophenyl-b-D-glucuronide Carrine Blank p-NP-b-D-glucuronide An assay for the activity of beta-glucuronidase in a microorganism. Uses the substrate 4-nitrophenyl-beta-D-glucuronide. Beta-glucuronidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. GUR PNPGLR bGUR 4-nitrophenyl-beta-D-glucuronide p-nitrophenyl-b-D-glucuronide N-acetyl-beta-glucosaminidase assay with pNP NAG p-NP-N-acetyl-b-D-glucosaminide Carrine Blank para-nitrophenyl-N-acetyl-beta-D-glucosaminide 4-nitrophenyl-N-acetyl-beta-D-glucosaminide p-nitrophenyl-N-acetyl-b-D-glucosaminide para-nitrophenyl-N-acetyl-b-D-glucosaminide An assay for the activity of N-acetyl-beta-glucosaminidase in a microorganism. Uses the substrate 4-nitrophenyl-N-acetyl-beta-D-glucosaminide. N-acetyl-beta-glucosaminidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. bNAG p-nitrophenyl-N-acetyl-beta-D-glucosaminide NAGA bNAP 4-nitrophenyl-N-acetyl-b-D-glucosaminide p-NP-N-acetyl-beta-D-glucosaminide novobiocin resistance assay An antibiotic resistance assay for the ability of a microorganism to utilize sugars in the presence of novobiocin. NOVO Carrine Blank glutamic acid decarboxylase assay The purpose of this test is to determine if a microbial isolate is capable of metabolizing glutamic acid under anaerobic conditions. When glutamic acid is metabolized, the pH of the medium decreases due to the accumulation of H+ and organic acids (e.g. glutamate, succinate). This turns color of the pH indicator (e.g. 4-nitrophenol) to yellow. A positive test yields a yellow color; a negative test is colorless. Glutamate decarboxylase carries out the following reaction: L-glutamate <=> 4-aminobutanoate (gamma-aminobutyrate) + CO2 Carrine Blank glutamic acid decarboxylase glutamic acid is decarboxylated GDC WIkipedia:Glutamate decarboxylase glutamate decarboxylase alpha-L-fucosidase assay with pNP aFUC para-nitrophenyl-a-L-fucopyranoside p-nitrophenyl-alpha-L-fucopyranoside 4-nitrophenyl-alpha-L-fucopyranoside An assay for the activity of alpha-fucosidase in a microorganism. Uses the substrate 4-nitrophenyl-alpha-L-fucopyranoside. Alpha-fucosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. Carrine Blank p-nitrophenyl-a-L-fucopyranoside 4-nitrophenyl-a-L-fucopyranoside para-nitrophenyl-alpha-L-fucopyranoside p-NP-a-L-fucopyranoside p-NP-alpha-L-fucopyranoside L-arginine arylamidase assay using NA L-arginyl-2-naphthylamide An L-arginine arylamidase assay that uses the substrate L-arginine-2-naphthylamide (L-arginine-beta-naphthylamide) at pH 7.5. Arginine arylamidase activity (which could be from arginine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-arginine arylamidase L-arginine aminopeptidase argininic arylamidase Carrine Blank ArgA L-arginyl-b-naphthylamide L-arginine-2-naphthylamide arginine-beta-naphthylamide L-arginyl-beta-naphthylamide L-proline arylamidase assay using NA Uses the substrate L-proline-2-naphthylamide at pH 7.5. Proline arylamidase activity (which could be from proline aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-prolyl-2-naphthylamide L-prolyl-beta-naphthylamide L-prolyl-b-naphthylamide Carrine Blank L-proline-2-naphthylamide leucyl glycine arylamidase assay leucyl glycine arylamidase LGA L-leucyl-glycyl-beta-naphthylamide L-leucyl-L-glycine-2-naphthylamide leucyl-glycine-beta-naphthylamide LGLY An amino acid arylamidase assay that assays for leucyl glycine arylamidase activity. Uses the substrate L-leucyl-L-glycine-2-naphthylamide at pH 7.5. Leucyl glycine arylamidase activity (which could be from leucyl glycine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. Carrine Blank leucyl glycine aminopeptidase L-leucyl-glycyl-b-naphthylamide L-phenylalanine arylamidase assay An alpha-amino acid arylamidase assay that uses the substrate L-phenylalanine-2-naphthylamide at pH 7.5. Phenylalanine arylamidase activity (which could be from phenoylalanine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-phenylalanine AMC phenylalanine arylamidase L-phenylalanyl-beta-naphthylamide phenylalanine aminopeptidase Carrine Blank L-phenylalanyl-b-naphthylamide L-phenylalanine 7-amido-4-methylcoumarin PheA L-phenylalanyl-2-naphthylamide L-phenylalanine-2-naphthylamide L-pyrrolidonyl arylamidase assay L-pyrrolidonyl-beta-naphthylamide PYRA L-pyroglutamic acid AMC L-pyrrolidonyl-b-naphthylamide An alpha-amino acid arylamidase assay that uses the substrate pyroglutamic acid-2-naphthylamide (pyroglutamic acid-beta-naphthylamide) at pH 7.5. Pyroglutamic acid arylamidase activity (which could be from pyroglutamic acid aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. pyrrolidonyl arylamidase PYR L-Pyroglutamic acid 7-amido-4-methylcoumarin L-pyrrolydonyl arylamidase Carrine Blank pyrrolidonyl aminopeptidase L-tyrosine arylamidase assay tyrosine aminopeptidase L-tyrosyl-beta-naphthylamide An alpha-amino acid arylamidase assay that uses the substrate L-tyrosine-2-naphthylamide at pH 7.5. Tyrosine arylamidase activity (which could be from tyrosine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. tyrosine arylamidase L-tyrosyl-b-naphthylamide L-tyrosine-2-naphthylamide TyrA L-tyrosyl-2-naphthylamide Carrine Blank TYR glycine arylamidase assay glycine aminopeptidase glycyl-beta-naphthylamide glycine-2-naphthylamide Carrine Blank An alpha-amino acid arylamidase assay that uses the substrate glycine-2-naphthylamide at pH 7.5. Glycine arylamidase activity (which could be from glycine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-glycine AMD glycine arylamidase glycine-beta-naphthylamide glycyl-b-naphthylamide L-glycine-2-naphthylamide GlyA glycyl-2-naphthylamide L-glycine 7-amino-4-coumarin L-histidine arylamidase assay L-histidine-2-naphthylamide L-histidyl-2-naphthylamide L-histidyl-b-naphthylamide histidine aminopeptidase L-histidine 7-amino-4-methylcoumarin Carrine Blank L-histidine AMD An alpha-amino acid arylamidase assay that uses the substrate L-histidine-2-naphthylamide at pH 7.5. Histidine arylamidase activity (which could be from histidine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. HisA L-histidyl-beta-naphthylamide histidine arylamidase glutamyl glutamic acid arylamidase assay Glutamyl-glutamic acid-2-naphthylamide glutamyl glutamic acid arylamidase L-glutamyl-L-glutamic acid-2-naphthylamide An amino acid arylamidase assay that assays for Glutamyl glutamic arylamidase activity. Uses the substrate L-glutamyl-L-glutamic acid-2-naphthylamide at pH 7.5. Glutamyl glutamic arylamidase activity (which could be from glutamyl glutamic aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. GGA glutamyl glutamic acid aminopeptidase Carrine Blank L-serine arylamidase assay L-seryl-beta-naphthylamide Carrine Blank L-seryl-b-naphthylamide L-seryl-2-naphthylamide L-serine-2-naphthylamide serine arylamidase An alpha-amino acid arylamidase assay that uses the substrate L-serine-2-naphthylamide at pH 7.5. Serine arylamidase activity (which could be from serine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. SerA serine aminopeptidase alanyl alanine arylamidase assay L-alanyl-L-alanine-b-naphthylamide L-alanyl-L-alanine-beta-naphthylamide L-alanyl-L-alanine-2-naphthylamide Carrine Blank alanyl alanine aminopeptidase An assay for alanyl alanine arylamidase activity. Uses the substrate L-alanyl-L-alanine-2-naphthylamide at pH 7.5. Alanyl alanine arylamidase activity (which could be from alanyl alanine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. AlaA alanyl alanine arylamidase beta-galactosidase assay bGAL beta-galactopyranosidase beta-D-galactosidase b-galactosidase beta-galactosidase Wikipedia:Beta-galactosidase Carrine Blank BGAR b-galactoside A carbohydrate hydrolysis assay for the activity of beta-galactosidase in a microorganism using various chromogenic substrates. beta-galactoside beta-glucosidase assay An assay for the activity of beta-glucosidase in a microorganism using various chromogenic substrates. Beta-glucosidase hydrolyses oligosaccharides (such as cellobiose) into monosaccharides. cellobiase beta-1,6-glucosidase Wikipedia:Beta-glucosidase beta-glucosidase beta-D-GLU BGLU b-D-GLU Carrine Blank beta-D-glucosidase arbutinase beta-D-glucoside b-glucosidase gentiobiase b-D-glucoside EC:3.2.1.21 beta-glucuronidase assay bGUR beta-glucuronidase b-glucoronidase Wikipedia:Beta-glucuronidase b-glucuronidase beta-glucoronidase Carrine Blank A carbohydrate hydrolysis assay for the activity of beta-glucuronidase in a microorganism using various chromogenic substrates. N-acetyl-beta-glucosaminidase assay b-N-acetyl-glucosaminidase Carrine Blank bNAG N-acetyl-glucosaminidase beta-N-acetyl-glucosaminidase N-acetyl-b-glucosaminidase N-acetyl-beta-glucosaminidase A carbohydrate hydrolysis assay for the activity of N-acetyl-beta-glucosaminidase in a microorganism using various chromogenic substrates. BNAG API 20 Strep API® 20 Strep; Identification system for Streptococcaceae and related organisms; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. Carrine Blank hippurate assay hippuric acid Carrine Blank HIP hippurate hydrolysed hippuric acid test hippurate test An assay for the hydrolysis of hippuric acid (benzoylaminoethanoic acid; N-benzoylglycine) in a microorganism. The test uses ninhydrin as a color indicator. Hippuric acid hydrolysis releases glycine, which reacts with ninhydrin (colorless) to form a purple color. A positive test result is dark blue/violet; a negative test result is colorless/pale blue/blueish-grey. hippurate hydrolysis API NH API® NH; System for the identification of Neisseria and Haemophilus; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. Carrine Blank lipase C10 assay Carrine Blank 5-bromo-3-indoxyl-caprate Uses the substrate 5-bromo-3-indoxyl-caprate (5-bromo-3-indolyl decanoate; blue-caprate). Lipases that can hydrolyze C10 compounds will cleave the substrate, producing 5-bromo-3-hydroxyindole. The latter will spontaneously dimerize and oxidize to 5,5'-dibromo-indigo, an intensely blue insoluble product. A postive reaction is blue with a precipitate; a negative reaction is colorless to pale grey. LIP 5-bromo-3-indoxyl-caproic acid Kiernan JA. 2007. Indigogenic substrates for detection and localization of enzymes. Biotechnic & Histochemistry 82(2):73-103. penicillinase assay Carrine Blank PEN An antibiotic resistance assay for the activity/presence of penicillinase using potassium benzylpenicillin (penicillin G) and starch iodide. Penicillin hydrolysis results in the decolorization of starch iodide due to the reduction of iodine. A positive reaction (penicillinase presence) is yellow, yellow-green, or yellow-blue. A negative reaction is blue. Perret CJ. 1954. Iodometric assay of penicillinase. Nature 174:1012-1013. gamma glutamyl transferase assay using methoxy-NA Carrine Blank gamma-glutamyl-4-methoxy-beta-naphthylamide gamma-glutamyl-4-methoxy-2-naphthylamide g-glutamyl-4-methoxy-beta-naphthylamide g-glutamyl-4-methoxy-2-naphthylamide g-glutamyl-4-methoxy-b-naphthylamide An amino acid arylamidase assay that measures the activity of gamma glutamyl transferase using gamma-glutamyl-4-methoxy-beta-naphthylamide. Gamma glutamyl transferase activity reacts with the substrate releasing beta-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive test is orange/dark orange; a negative test is colorless, yellow to pale orange (may be yellow orange if the organism is positive for alkaline phosphatase). gamma-glutamyl-4-methoxy-b-naphthylamide GGT malonate fermentation/oxidation assay MNT Carrine Blank Assays for the ability of a microorganism to utilize malonate. API RapiD 20 E API® Rapid ID 20 E – System for the identification of Enterobacteriaceae in 4 hours; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. Carrine Blank para-phenylalanine deaminase assay Measures the presence of para-phenylalanine deaminase activity using the substrate 4-nitrophenylalanine. Para-phenylalanine deaminase activity cleaves the compound releasing ammonia and 3-(4-nitrophenyl)propanoic acid (yellow to brown). A positive reaction is yellow to brown; a negative reaction is colorless. Carrine Blank PPA para-phenylalanine deaminase API Coryne API® Coryne; Identification system for coryneform bacteria ; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. Carrine Blank pyrazinamidase assay pyrazinamidase An enzymatic assay which tests for the presence of pyrazinamidase activity using the substrate pyrazinecarboxamide. Pyrazinamidase hydrolyzes the substrate pyrazinamide (pyrazinecarboxamide) to form pyrazinoic acid (pyrazine-2-carboxylic acid). In the presence of ferrous ammonium sulfate a complex forms witht he product to form a "rusty pink color complex". A positive test is brown orange; a negative test is colorless/very pale brown/very pale orange. Pyrazinamidase catalyzes the following reaction: Pyrazinamide + H2O <=> pyrazinoic acid (pyrazine-2-carboxylic acid) + NH3. Revathi G & Talwar V. 1995. A biochemical test based on pyrazinamidase activity for rapid differentiation of Corynebacteria. Indian J Clin Biochem 10(1):39-41. pyrazinamidase test PYZ EC:3.5.1.B15 Carrine Blank alpha-glucosidase assay a-glucosidase aGLU An assay for the activity of alpha-glucosidase in a microorganism using various chromogenic substrates. Alpha glucosidase acts on 1,4-alpha bonds in polymers of glucose. alpha-D-glucoside alpha-glucosidase Carrine Blank a-D-glucoside alpha-glucosidase assay with naphthol 2-naphthyl-alpha-D-glucopyranoside aGLU An assay for the activity of alpha-glucosidase in a microorganism. Uses the substrate 2-naphthyl alpha-D-glucopyranoside. Alpha-glucosidase will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is purple in color. A positive result is purple; a negative result is colorless, beige-pale purple, pale green. 2-naphthyl-a-D-glucopyranoside Carrine Blank API Campy Carrine Blank API® Campy; Identification system for Campylobacter; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. esterase C2 assay Carrine Blank 5-bromo-4-chloro-3-indoxyl-acetate Test for C2 esterase activity in a microorganism using the substrate 5-bromo-4-chloro-3-indoxyl-acetate. Esterase that can hydrolyze C2 compounds will cleave the substrate, producing 5-bromo-4-chloro-3-hydroxyindole. The later will spontaneously dimerize and oxidize to 5,5'-dibromo-4,4'-dichloro-indigo, an intensely blue insoluble product. A postive reaction is blue/turquiose with a precipitate; a negative reaction is colorless to pale blue. Kiernan JA. 2007. Indigogenic substrates for detection and localization of enzymes. Biotechnic & Histochemistry 82(2):73-103. EST L-aspartate arylamidase assay L-aspartate-2-naphthylamide Carrine Blank ApsA L-aspartyl-2-naphtylamide An alpha-amino acid arylamidase assay that uses the substrate L-aspartate-2-naphthylamide (L-aspartic acid-beta-naphthylamide) at pH 7.5. Aspartate arylamidase activity (which could be from aspartate aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-aspartyl-beta-naphthylamide L-aspartyl-b-naphthylamide L-aspartate arylamidase aspartate aminopeptidase aspartate arylamidase succinic acid assimilation assay SUT succinic acid assimilation sodium succinate succinic succinic acids succinate utilization disodium succinate Assays for the ability of a microorganism to assimilate succinate (succinic acid) as a sole source of carbon and energy. succinate assimilation succinate succinic acid utilization succinic acid Carrine Blank antibiotic resistance assay Carrine Blank An assay for the ability of a microorganism to grow or utilize sugars in the presence of an antibiotic (an antimicrobial compound). nalidixic acid resistance assay nalidixic acid An antibiotic resistance assay for the ability of a microorganism to grow in the presence of nalidixic acid. Carrine Blank NAL cefazolin resistance assay Carrine Blank cefazoline An antibiotic resistance assay for the ability of a microorganism to grow in the presence of (sodium) cefazolin. CFZ cefazolin erythromycin resistance assay An antibiotic resistance assay for the ability of a microorganism to grow in the presence of erythromycin. Carrine Blank ERO alpha-galactosidase assay Carrine Blank a-galactosidase alpha-D-GAL a-D-GAL alpha-D-galactosidase A carbohydrate hydrolysis assay for the activity of alpha-galactosidase in a microorganism using various chromogenic substrates. a-D-galactosidase alpha-galactosidase aGAL AGAL alpha-galactosidase assay with BrNaphthol aGAL 6-Br-2-naphthyl-a-D-galactopyranoside 6-bromo-2-naphthyl-alpha-D-galactopyranoside An assay for the activity of alpha-galactosidase in a microorganism. Uses the substrate 6-bromo-2-naphthyl-alpha-D-galactopyranoside. Alpha-galactosidase will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is purple in color. A positive result is purple; a negative result is colorless. Carrine Blank 6-bromo-2-naphthyl-a-D-galactopyranoside 6-Br-2-naphthyl-alpha-D-galactopyranoside N-acetyl-beta-glucosaminidase assay with oNP 2-nitrophenyl-N-acetyl-b-D-glucosaminide o-NP-N-acetyl-b-D-glucosaminide An assay for the activity of N-acetyl-beta-glucosaminidase in a microorganism. Uses the substrate 2-nitrophenyl-N-acetyl-beta-D-glucosaminide (o-nitrophenyl-N-acetyl-beta-D-glucosaminide). N-acetyl-beta-glucosaminidase will cleave the substrate, producing 2-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. o-NP-N-acetyl-beta-D-glucosaminide ortho-nitrophenyl-N-acetyl-b-D-glucosaminide 2-nitrophenyl-N-acetyl-beta-D-glucosaminide o-nitrophenyl-N-acetyl-b-D-glucosaminide ortho-nitrophenyl-N-acetyl-beta-D-glucosaminide Carrine Blank o-nitrophenyl-N-acetyl-beta-D-glucosaminide amino acid metabolism assay An enzymatic assay which tests for the ability of a microorganism to metabolize (for example, by hydrolysis or decarboxylation) or amino acid-containing substrates. Carrine Blank amino acid arylamidase assay Amino acid metabolism assay which tests for the ability of a microorganism to hydrolyze amino acid substrates coordinated with a beta-naphthylamide (2-naphthylamide) moiety. Arylamidases are classes of enzymes with the ability to hydrolyze amino acid-beta-naphtylamide substrates. Sheahan JP, Eitenmiller RR, & Carpenter JA. 1975. Arylamidase activity of Salmonella species. Appl Microbiol 29(6):726-728. aminopeptidase arylamidase Carrine Blank amino acid decarboxylase assay acid acids decarboxylated Carrine Blank Amino acid metabolism assay which tests for the ability of a microorganism to grow/metabolize using an amino acid as a source of energy. electromagnetic radiation Carrine Blank Wikipedia: Electromagnetic radiation Electromagnetic radiation (EM radiation or EMR) is a form of radiant energy released by certain electromagnetic processes. Visible light is one type of electromagnetic radiation, other familiar forms are invisible electromagnetic radiations such as X-rays and radio waves. Radiant energy released by some electromagnetic processes, including visible light. amino acid deaminase assay Amino acid metabolism assay which tests for the ability of a microorganim to gain energy by the deamination of an amino acid. Carrine Blank carbohydrate hydrolysis assay Carrine Blank An enzymatic assay which tests for the ability of a microorganism to hydrolyze carbohydrates or carbohydrate derivatives. GLY1 protein metabolism assay An enzymatic assay which tests for the ability of a microorganism to hydrolyze proteins or oligopeptides. Carrine Blank redox assay An assay for the ability of a microorganism to oxidize or reduce specific chemical compounds. Carrine Blank milk agar Carrine Blank An organic-rich, solid microbiological culture medium that contains tryptone, yeast extract, skim milk powder, and glucose. Used for the cultivation of microorganisms in dairy products. From: Milk Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Milk Agar is recommended by the British Standards Institute1 for the enumeration of microorganisms in liquid milk, ice cream, dried milk and whey. Principles of Procedure Peptone and yeast extract provide essential nutrients while skim milk powder is a source of casein. Dextrose is the carbon energy source. Agar is the solidifying agent. Proteolytic bacteria will be surrounded by a clear zone from the conversion of casein into soluble nitrogenous compounds.1 Formula Difco™ Milk Agar Approximate Formula* Per Liter Tryptone...................................................................... 5.0 g Yeast Extract................................................................ 2.5 g Dextrose...................................................................... 1.0 g Skim Milk Powder (antibiotic free)................................ 1.0 g Agar.......................................................................... 12.5 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.9 ± 0.1 L-alanine arylamidase assay L-alanyl-b-naphthylamide ALA An alpha-amino acid arylamidase assay that uses the substrate alanine-2-naphthylamide (L-alanine-beta-naphthylamide) at pH 7.5. Alanine arylamidase activity (which could be from alanine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. Test is also performed using L-alanine-p-nitroanilide. Alanine arylamidase activity cleaves the substrate, releasing p-nitroaniline which is bright yellow in color. A positive test is yellow; a negative test is colorless. Carrine Blank L-alanine 7-amido-4-methylcoumarin L-alanyl-beta-naphthylamide AlaA alanine aminopeptidase alanine arylamidase L-alanine-2-naphthylamide L-alanine-p-nitroanilide L-alanine-p-NA L-alaninyl-2-naphthylamide L-alanine AMD L-asparagine arylamidase assay L-asparagyl-b-naphthylamide L-asparagyl-beta-naphthylamide An alpha-amino acid arylamidase assay that uses the substrate L-asparagine-2-naphthylamide (L-asparaginyl-beta-naphthylamide) at pH 7.5. Asparagine arylamidase activity (which could be from asparagine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. asparagine aminopeptidase asparagine arylamidase L-asparagine-2-naphthylamide Carrine Blank L-asparaginyl-2-naphthylamide L-alpha-glutamate arylamidase assay alpha-glutamate aminopeptidase a-L-glutamyl-b-naphthylamide Uses the substrate L-alpha-glutamate-2-naphthylamide (L-alpha-glutamyl-beta-naphthylamide) at pH 7.5. Alpha-glutamate arylamidase activity (which could be from alpha-glutamate aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. alpha-glutamate arylamidase alpha-glutamic acid arylamidase L-alpha-glutamyl-beta-naphthylamide L-alpha-glutamyl-b-naphthylamide alpha-L-glutamyl-beta-naphthylamide a-L-glutamyl-beta-naphthylamide Carrine Blank alpha-glutamic acid aminopeptidase alpha-L-glutamyl-b-naphthylamide gamma-glutamyl arylamidase assay using NA GGT Carrine Blank gamma-glutamyl-2-naphthylamide gamma-glutamyl-beta-naphthylamide gamma-glutamyl-b-naphthylamide Uses the substrate gamma-glutamate-2-naphthylamide (gamma-glutamyl-beta-naphthylamide) at pH 7.5. Gamma-glutamate arylamidase activity (which could be from gamma-glutamate aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-hydroxy proline arylamidase assay L-hydroxyprolyl-2-naphthylamide Carrine Blank An alpha-amino acid arylamidase assay that uses the substrate L-hydroxyproline-2-naphthylamide (L-hydroxyprolyl-beta-naphthylamide) at pH 7.5. Hydroxyproline arylamidase activity (which could be from hydroxyproline aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. hydroxy proline aminopeptidase HPR L-hydroxyprolyl-beta-naphthylamide L-hydroxyprolyl-b-naphthylamide hydroxy proline arylamidase L-hydroxyproline-2-naphthylamide L-lysine arylamidase assay An alpha-amino acid arylamidase assay that uses the substrate L-lysine-2-naphthylamide (L-lysyl-beta-naphthylamide) at pH 7.5. Lysine arylamidase activity (which could be from lysine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. lysine aminopeptidase LysA Carrine Blank L-lysyl-2-naphthylamide L-lysyl-b-naphthylamide L-lysyl-beta-naphthylamide lysine arylamidase LYS L-lysine-2-naphthylamide L-methionine arylamidase assay L-methionyl-b-naphthylamide Carrine Blank Uses the substrate L-methionine-2-naphthylamide (L-methionyl-beta-naphthylamide) at pH 7.5. Methionine arylamidase activity (which could be from methionine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-methionine aminopeptidase L-methionyl-beta-naphthylamide L-methionine arylamidase L-methionyl-2-naphthylamide L-methionine-2-naphthylamide 4-methoxy leucine arylamidase assay methoxy leucine aminopeptidase Uses the substrate L-leucine-4-methoxy-2-naphthylamide (4-methoxy-leucyl-beta-naphthylamide, L-leucine 4-methoxy-beta-naphthylamide) at pH 7.5. Methoxy leucine arylamidase activity (which could be from methoxy leucine aminopeptidase, aminopeptidase M, or leucine aminopeptidase, as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. 4-methoxy-leucyl-b-naphthylamide 4-methoxy-leucyl-beta-naphthylamide L-leucine-4-methoxy-b-naphthylamide L-leucine-4-methoxy-beta-naphthylamide methoxy leucine arylamidase L-leucine-4-methoxy-2-naphthylamide Carrine Blank L-ornithine arylamidase assay ornithine aminopeptidase L-ornithine-2-naphthylamide L-ornithyl-b-naphthylamide L-ornithyl-beta-naphthylamide An alpha-amino acid arylamidase assay that uses the substrate L-ornithine-2-naphthylamide (L-ornithyl-beta-naphthylamide) at pH 7.5. Ornithine arylamidase activity (which could be from ornithine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. ornithine arylamidase L-ornithyl-2-naphthylamide Carrine Blank L-threonine arylamidase assay L-threonyl-2-naphthylamide An alpha-amino acid arylamidase assay that uses the substrate L-threonine-2-naphthylamide (L-threonyl-beta-naphthylamide) at pH 7.5. Threonine arylamidase activity (which could be from threonine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-threonine-2-naphthylamide threonine aminopeptidase L-threonyl-beta-naphthylamide threonine arylamidase Carrine Blank L-threonyl-b-naphthylamide L-tryptophan arylamidase assay L-tryptophan-2-naphthylamide An alpha-amino acid arylamidase assay that uses the substrate L-tryptophan-2-naphthylamide (L-tryptophyl-beta-naphthylamide) at pH 7.5. Tryptophan arylamidase activity (which could be from tryptophan aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. TRY L-tryptophyl-b-naphthylamide L-tryptophyl-2-naphthylamide Carrine Blank tryptophan aminopeptidase L-tryptophyl-beta-naphthylamide tryptophan arylamidase L-glutamine arylamidase assay glutamine arylamidase glutamine aminopeptidase Carrine Blank L-glutaminyl-2-naphthylamide AGLTp L-glutaminyl-b-naphthylamide L-glutamine-b-naphthylamide L-glutamine-2-naphthylamide L-glutamine-beta-naphthylamide L-glutaminyl-beta-naphthylamide An alpha-amino acid arylamidase assay that uses the substrate L-glutamine-2-naphthylamide (L-glutaminyl-beta-naphthylamide) at pH 7.5. Glutamine arylamidase activity (which could be from glutamine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-isoleucine arylamidase assay isoleucine aminopeptidase An alpha-amino acid arylamidase assay that uses the substrate L-isoleucyl-2-naphthylamide (L-isoleucine-beta-naphthylamide) at pH 7.5. Isoleucine arylamidase activity (which could be from isoleucine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-isoleucyl-b-naphthylamide isoleucine arylamidase L-isoleucyl-beta-naphthylamide L-isoleucyl-2-naphthylamide Carrine Blank L-isoleucine-2-naphthylamide seryl tyrosine arylamidase assay L-serine-L-tyrosine-2-naphthylamide L-seryl-L-tyrosyl aminopeptidase L-seryl-L-tyrosyl-beta-naphthylamide L-seryl-L-tyrosyl arylamidase Carrine Blank L-seryl-L-tyrosyl-b-naphthylamide An amino acid arylamidase assay that assays for seryl tyrosine arylamidase activity. Uses the substrate L-seryl-L-tyrosine-2-naphthylamide at pH 7.5. Seryl tyrosine arylamidase activity (which could be from seryl tyrosine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-seryl-L-tyrosine-2-naphthylamide glycyl proline arylamidase assay An amino acid arylamidase assay that assays for glycyl proline arylamidase activity. Uses the substrate glycyl-L-proline-2-naphthylamide at pH 7.5. Glycyl proline arylamidase activity (which could be from glycyl proline aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. glycyl-L-proline-2-naphthylamide Carrine Blank glycyl proline aminopeptidase glycyl-L-prolyl-b-naphthylamide glycyl proline arylamidase glycyl-L-prolyl-beta-naphthylamide glycyl-L-prolyl-2-naphthylamide glycyl phenylalanine arylamidase assay glycyl-L-phenylalanine-2-naphthylamide glycyl phenylalanine arylamidase glycyl-L-phenylalanyl-beta-naphthylamide glycyl-L-phenylalanyl-b-naphthylamide glycyl-L-phenylalanyl-2-naphthylamide Carrine Blank glycyl phenylalanine aminopeptidase An amino acid arylamidase assay that assays for glycyl phenylalanine arylamidase activity. Uses the substrate glycyl-L-phenylalanine-2-naphthylamide at pH 7.5. Glycyl phenylalanine arylamidase activity (which could be from glycyl phenylalanine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. DL-methionine arylamidase assay methionine aminopeptidase An alpha-amino acid arylamidase assay that uses the substrate DL-methionine-2-naphthylamide (DL-methionyl-beta-naphthylamide) at pH 7.5. DL-Methionine arylamidase activity (which could be from methionine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. DL-methionine-2-naphthylamide DL-methionyl-2-naphthylamide DL-methionyl-b-naphthylamide methionine arylamidase DL-methionyl-beta-naphthylamide Carrine Blank glutaryl phenylalanine arylamidase assay Carrine Blank N-glutaryl-L-phenylalanine-2-naphthylamide glutaryl-L-phenylalanyl-beta-naphthylamide N-glutaryl-phenylalanine-2-naphthylamide glutaryl-L-phenylalanyl-2-naphthylamide glutaryl phenylalanine aminopeptidase glutaryl-L-phenylalanyl-b-naphthylamide glutaryl-L-phenylalanine-2-naphthylamide glutaryl phenylalanine arylamidase An amino acid arylamidase assay that assays for glutaryl phenylalanine arylamidase activity. Uses the substrate glutaryl-L-phenylalanine-2-naphthylamide at pH 7.5. Glutaryl phenylalanine arylamidase activity (which could be from glutaryl phenylalanine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. N-carbobenzoyl-glycyl-glycyl-L-arginine peptidase assay N-carbobenzoyl-glycyl-glycyl-arginyl-2-naphthylamide Carrine Blank An amino acid arylamidase assay that assays for the presence of N-carbobenzoyl-glycyl-glycyl-arginine-beta-naphthylamide peptidase in a microorganism. Uses N-carbobenzoyl-glycyl-glycyl-arginine-beta-naphthylamide as a substrate. Hydrolysis of the substrate releases 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. N-carbobenzoxy-glycyl-glycyl-L-arginine-beta-naphthylamide N-carbobenzoyl-glycyl-glycyl-L-arginine-2-naphthylamide N-carbobenzoyl-glycyl-glycyl-arginyl-b-naphthylamide N-carbobenzoyl-glycyl-glycyl-arginyl-beta-naphthylamide N-carbobenzoyl-glycyl-glycyl-arginine-2-naphthylamide N-benzoyl-L-valyl-glycyl-L-arginine-4-methoxy peptidase assay N-benzoyl-L-valyl-glycyl-4-methoxy-beta-naphthylamide N-benzoyl-L-valyl-glycyl-4-methoxy-2-naphthylamide An amino acid arylamidase assay that assays for the presence of N-benzoyl-L-valyl-glycyl-4-methoxy-beta-naphthylamide peptidase in a microorganism. Uses N-benzoyl-L-valyl-glycyl-4-methoxy-beta-naphthylamide as a substrate. Hydrolysis of the substrate releases 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. N-benzoyl-L-valyl-glycyl-4-methoxy-b-naphthylamide peptidase assay protease protease test Carrine Blank protein hydrolysis proteinase test An assay for the ability of a microorganism to hydrolyze proteins. L-fucosidase assay alpha-fucosidase alpha-L-fucosidase Carrine Blank An assay for the activity of alpha-fucosidase in a microorganism using various chromogenic substrates which carries out the following reaction: alpha-L-fucoside + H2O <=> L-fucose + an alcohol aFUC a-fucosidase N-benzoyl-L-leucine peptidase assay An amino acid arylamidase assay that assays for the presence of N-benzoyl-L-leucine-beta-naphthylamide peptidase in a microorganism. Uses N-benzoyl-L-leucine-beta-naphthylamide as a substrate. Hydrolysis of the substrate releases 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. N-benzoyl-L-leucyl-2-naphthylamide N-benzoyl-L-leucyl-beta-naphthylamide N-benzoyl-L-leucyl-b-naphthylamide N-benzoyl-L-leucine-beta-naphthylamide Carrine Blank alpha-L-fucosidase assay with naphthol 2-naphthyl-a-L-fucopyranoside 2-naphthyl-alpha-L-fucopyranoside An assay for the activity of alpha-fucosidase in a microorganism. Uses the substrate 2-naphthyl-alpha-L-fucopyranoside. Alpha-fucosidase will cleave the substrate, producing 2-napthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is purple in color. A positive result is purple; a negative result is colorless. Carrine Blank N-acetyl-alpha-D-galactosaminidase assay alpha-N-acetyl-galactosaminidase N-acetyl-a-D-galacotsaminidase N-acetyl-alpha-galactosaminidase A carbohydrate hydrolysis assay for the activity of N-acetyl-alpha-galactosaminidase in a microorganism using various chromogenic substrates. Carrine Blank N-acetyl-alpha-D-galactosaminidase assay with pNP para-nitrophenyl-N-acetyl-alpha-D-galactosaminide Carrine Blank p-nitrophenyl-N-acetyl-alpha-D-galactosaminide 4-nitrophenyl-N-acetyl-a-D-galactosaminide p-nitrophenyl-N-acetyl-a-D-galactosaminide 4-nitrophenyl-N-acetyl-alpha-D-galactosaminide para-nitrophenyl-N-acetyl-a-D-galactosaminide An assay for the activity of N-acetyl-alpha-galactosaminidase in a microorganism. Uses the substrate 4-nitrophenyl-N-acetyl-alpha-D-galactosaminide (p-nitrophenyl-N-acetyl-alpha-D-galactosaminide). N-acetyl-alpha-galactosaminidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. N-acetyl-alpha-D-galactosaminidase assay with oNP Carrine Blank o-NP-N-acetyl-a-D-galactosaminide 2-NP-N-acetyl-alpha-D-galactosaminide An assay for the activity of N-acetyl-alpha-galactosaminidase in a microorganism. Uses the substrate 2-nitrophenyl-N-acetyl-alpha-D-galactosaminide (o-nitrophenyl-N-acetyl-alpha-D-galactosaminide). N-acetyl-alpha-galactosaminidase will cleave the substrate, producing 2-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. o-nitrophenyl-N-acetyl-alpha-D-galactosaminide 2-nitrophenyl-N-acetyl-alpha-D-galactosaminide o-nitrophenyl-N-acetyl-a-D-galactosaminide 2-nitrophenyl-N-acetyl-a-D-galactosaminide o-NP-N-acetyl-alpha-D-galactosaminide 2-NP-N-acetyl-a-D-galactosaminide beta-xylosidase assay using pNP p-NP-beta-D-xylopyranoside bXYL BXYL beta-D-xylopyranosidase 4-nitrophenyl-b-D-xylopyranoside para-nitrophenyl-beta-D-xylopyranoside 4-nitrophenyl-beta-D-xylopyranoside pNP-beta-D-xyloside p-nitrophenyl-b-D-xylopyranoside pNP-b-D-xyloside beta-xylopyranosidase p-NP-b-D-xylopyranoside b-xylosidase para-nitrophenyl-b-D-xylopyranoside p-nitrophenyl-beta-D-xylopyranoside A carbohydrate hydrolysis assay for the activity of beta-xylosidase in a microorganism using the substrate 4-nitrophenyl-beta-D-xylopyranoside. Beta-xylosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. beta-xylosidase alpha-xylosidase assay p-nitrophenyl-a-D-xylopyranoside A carbohydrate hydrolysis assay for the activity of alpha-xylosidase in a microorganism using the substrate 4-nitrophenyl-alpha-D-xylopyranoside. Alpha-xylosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. 4-nitrophenyl-alpha-D-xylopyranoside a-xylosidase p-NP-a-D-xylopyranoside p-nitrophenyl-alpha-D-xylopyranoside 4-nitrophenyl-a-D-xylopyranoside 4-NP-a-D-xylopyranoside p-NP-alpha-D-xylopyranoside alpha-xylosidase alpha-xylopyranosidase alpha-D-xylopyranosidase 4-NP-alpha-D-xylopyranoside beta-L-fucosidase assay Carrine Blank An assay for the activity of beta-L-fucosidase in a microorganism using various chromogenic substrates. beta-L-fucosidase beta-L-fucopyranosidase beta-L-fucosidase assay with pNP 4-nitrophenyl-b-L-fucoside 4-nitrophenyl-beta-L-fucopyranoside 4-nitrophenyl-b-L-fucopyranoside An assay for the activity of beta-L-fucosidase in a microorganism. Uses the substrate 4-nitrophenyl-beta-L-fucopyranoside. Beta-L-fucosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. p-nitrophenyl-b-L-fucopyranoside p-NP-b-L-fucopyranoside Carrine Blank p-nitrophenyl-beta-L-fucopyranoside p-NP-beta-L-fucopyranoside 4-nitrophenyl-beta-L-fucoside acid phosphatase assay Wikipedia:Acid_phosphatase Carrine Blank Acid phosphatase catalyzes the following reaction (under acidic pH conditions): A phosphate monoester + H2O <=> an alcohol + phosphate acid phosphatase D-fucosidase assay beta-fucosidase b-fucosidase Carrine Blank An assay for the activity of beta-fucosidase in a microorganism using various chromogenic substrates. beta-D-fucosidase assay using pNP Carrine Blank p-nitrophenyl-beta-D-fucopyranoside 4-nitrophenyl-beta-D-fucopyranoside 4-nitrophenyl-b-D-fucopyranoside An assay for the activity of beta-D-fucosidase in a microorganism. Uses the substrate 4-nitrophenyl-beta-D-fucopyranoside. Beta-D-fucosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. p-nitrophenyl-b-D-fucopyranoside acid phosphatase assay with pNP bis-(p-nitrophenyl)phosphate Uses the substrate bis-para-nitrophenyl-phosphate (para-nitrophenylphosphate, 4-nitrophenyl phosphate). Under acidic conditions, acid phosphatase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. bis-para-nitrophenyl-phosphate bis-pNP-phosphate PNPP Carrine Blank para-nitrophenylphosphate bis-p-nitrophenyl-phosphate alkaline phosphatase assay Carrine Blank Wikipedia:Alkaline_phosphatase PAL An alkaline phosphatase catalyzes the following reaction (under alkaline pH conditions): A phosphate monoester + H2O <=> an alcohol + phosphate alkaline phosphatase alkaline phosphatase assay with pNP Carrine Blank bis-para-nitrophenyl-phosphate bis-p-nitrophenyl-phosphate PNPP bis-(p-nitrophenyl)phosphate bis-pNP-phosphate para-nitrophenylphosphate Alkaline phosphatase assay that uses the substrate bis-para-nitrophenyl-phosphate (para-nitrophenylphosphate, 4-nitrophenyl phosphate). Under alkaline conditions, alkaline phosphatase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. PAL phosphodiesterase assay Carrine Blank Wikipedia:Phosphodiesterase p-nitrophenyl-phosphoester phosphodiesterase phosphoric monoester hydrolase test An enzymatic assay which tests for the presence of phosphodiesterase - an enzyme that hydrolyzes a phosphodiester bonds (two ester bonds between a phosphate group and two carbohydrates, i.e. sugar-phosphate-sugar). EC:3.1.3 phosphonate hydrolase assay with pNP Carrine Blank OPS NPPP phenylphosphonate phosphonate monoester hydrolase p-NP-phenyl-phosphonate bis-p-nitrophenyl-phenyl-phosphonate phosphonate hydrolase Phosphonate hydrolase is an esterase that hydrolyzes a phosphonate compound (an organophosphorus compound with multiple alklyl or aryl groups). p-nitrophenyl phenyl phosphonate is a substrate (NPPP) for phosphonate hydrolase enzymes. Hydrolysis of this compound releases 4-nitrophenol (p-nitrophenol), which is yellow. A positive result is yellow; a negative result is colorless. beta-glucosidase assay with pNP-lactoside p-nitrophenyl-lactoside 4-nitrophenyl-b-D-lactopyranoside 4-nitrophenyl-beta-D-lactoside Carrine Blank 4-nitrophenyl-b-D-lactoside An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate 4-nitrophenyl-beta-D-lactopyranoside (lactopyranoside is a disaccharide comprised of lactose, or gal-glu). Beta-glucosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. p-nitrophenyl-beta-D-lactopyranoside 4-nitrophenyl-beta-D-lactopyranoside phospholipase C assay bis-p-nitrophenyl-phosphoryl-choline phosphoryl choline O-(4-nitrophenylphosphoryl)choline NPPC Carrine Blank pNP-phosphoryl-choline PHC phosphorylcholine p-nitrophenyl phosphoryl choline As assay for the activity of phospholipase C (phosphorylcholine hydrolase); an enzyme that catalyzes the hydrolysis of the glycerophosphate bond of phospholipids. Uses the substrate bis-p-nitrophenyl-phosphoryl-choline. Phospholipase C will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. arylsulfatase assay with pNC para-nitrocatechol sulphate EC:3.1.6.1 sulphatase p-nitrocatechol-sulfate pNCS para-nitrocatechol sulfate Carrine Blank 4-nitrocatechol sulphate Test for the presence of arylsulfatase (sulfatase) in a microorganism using the chromogenic substrate 4-nitrocatechol sulfate (pNCS, 2-hydroxy-5-nitrophenyl sulfate). The substrate is cleaved (via hydrolysis reaction) to form p-nitrocatechol + sulfate. p-nitrocatechol (4-nitrocatechol), under alkaline conditions, is red. A positive test is red; a negative test is yellow. p-nitrocathecol-sulfate sulfatase 2-hydroxy-5-nitrophenyl sulfate arylsulfatase arylsulphatase 2-hydroxy-5-nitrophenyl sulphate 4-nitrocatechol sulfate arylsulfatase assay Test for the presence of arylsulfatase (sulfatase) in a microorganism using a chromogenic substrate that contains an aryl group. Carrine Blank sulfatase assay An enzymatic assay which tests for the presence of sulfatase in a microorganism using a chromogenic substrate. Sulfatases (EC 3.1.6) are esterases that catalyze the hydrolysis of sulfate esters. Carrine Blank sulfatase test glucosidase assay A carbohydrate hydrolysis assay for the activity of alpha-glucosidase or beta-glucosidase in a microorganism using various chromogenic substrates. glucosidase Carrine Blank alpha-mannosidase assay Carrine Blank a-mannosidase AMAN alpha-mannosidase A carbohydrate hydrolysis assay for the activity of alpha-mannosidase in a microorganism using various chromogenic substrates. amylase assay amylase test Carrine Blank amylolytic amylase An assay for the activity of amylase in a microorganism using various chromogenic substrates. DNase assay Carrine Blank deoxyribonuclease DNase DNA hydrolysis An enzymatic assay which tests for the presence of DNase activity. fructose-1,6-bisphosphate aldolase assay A carbohydrate hydrolysis assay for the activity of fructose-1,6-bisphosphate aldolase (aldolase) using various chromogenic substrates. Catalyzes the reaction: fructose 1,6-bisphosphate <=> dihydroxyacetone phosphate (DHAP) + glyceraldehyde-3-phosphate (GAP) fructose-1,6-bisphosphate aldolase Carrine Blank aldolase organic molecular entity metabolic assay buffered single substrate test An assay for the assimilation, fermentation, oxidation, or hydrolysis of an organic molecular entity by a microorganism. bss test bss medium Carrine Blank bss uridine phosphorylase assay An enzymatic assay which tests for the preence of uridine phosphorylase activity in a microorganism. Uridine phosphorylase catalyzes the reaction: uridine + phosphate <=> uracil + alpha-D-ribose-1-phosphate uridine phosphorylase Carrine Blank esterase assay esterase A hydrolase assay for the presence of esterase activity (carboyxlic ester hydrolase activity) in a microorganism. Esterase enzymes split short-chained esters into an acid and an alcohol via a hydrolysis reaction. Carrine Blank 6-phosphogluconate dehydrogenase assay 6-phosphogluconate dehydrogenase A carbohydrate hydrolysis assay for the presence of 6-phosphogluconate dehydrogenase (EC: 1.1.1.43) in a microorganism. 6-phosphogluconate dehydrogenase is an enzyme in the pentose phosphate pathway and catalyzes the reaction: 6-phospho-D-gluconate + NAD(P) <=> 6-phospho-2-dehydro-D-gluconate + NAD(P)H + H+ Carrine Blank maltase assay Carrine Blank Assays for the presence of maltase in a microorganism. Maltase is an alpha-glucosidase that hydrolyzes the alpha,1-4 bond in maltose (a disaccharide comprised of glucose monomers). maltase alpha-maltosidase EC:3.2.1.20 agarase assay agar hydrolysis Wikipedia:Agarase A carbohydrate hydrolysis assay for the presence of agarase activity in a microorganism. Agarase (4-glycanohydrolase) hydrolizes alpha or beta linkages in agarose, produing oligosaccharides. Agar is a purified form of the polysaccharide agarose (a polymer of D-galactose and 3,6-anhydro-L-galactopyranose, linked by alpha 1->3 and beta 1-> 4 bonds). Carrine Blank hydrolysis of agar agarase beta-alanine arylamidase assay beta-alanine arylamidase Carrine Blank beta-alanine arylamidase-p-nitroanilide beta-alanine-p-nitroanilide An amino acid arylamidase assay that uses the substrate beta-alanine-2-naphthylamide (beta-alanine beta-naphthylamide) at pH 7.5. Beta-alanine arylamidase activity (which could be from beta-alanine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. Test is also performed using beta-alanine-p-nitroanilide. Beta-alanine arylamidase activity cleaves the substrate, releasing p-nitroaniline which is bright yellow in color. A positive test is yellow; a negative test is colorless. BAlap beta-alanine arylamidase pNA b-alanine arylamidase-p-niroanilide zzzz note: nitroanilide is not naphthylamide VITEK 2 test card Carrine Blank VITEK® 2 is a fully automated system that performs bacterial identification and antibiotic susceptibility testing; http://www.biomerieux-usa.com VITEK® is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. GN ID card Gram negative bacterial identification; http://www.biomerieux-usa.com VITEK® is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. Vitek 2GN system Carrine Blank GP ID card Gram positive bacterial identification; http://www.biomerieux-usa.com VITEK® is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. Carrine Blank YST ID card Carrine Blank Yeast identification; http://www.biomerieux-usa.com VITEK® is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. NH ID card Carrine Blank Neisseria, Haemophilus and other fastidious Gram negative bacteria identification; http://www.biomerieux-usa.com VITEK® is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. ANC ID card Carrine Blank Anaerobic bacteria and coryneform bacteria identification; http://www.biomerieux-usa.com VITEK® is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. erythritol assimilation assay i-erythritol erythrol DL-erythritol ERYa erythritol assimilation meso-erythritol erythritol Carrine Blank erythrotol mesoerythritol erythitol Assays for the ability of a microorganism to assimilate erythritol as a sole source of carbon and energy. glycerol assimilation assay Carrine Blank gkycerol GLYLa glycerol glycerol assimilation Assays for the ability of a microorganism to assimilate glycerol as a sole source of carbon and energy. glycerin glucerol hydroquinone O-beta-D-glucopyranoside assimilation assay Carrine Blank arubtin arubutin ARBa p-arbutin arbutine assimilation arbutine L-arbutin arbutin assimilation 4-hydroxyphenyl-beta-D-glucopyranoside Assays for the ability of a microorganism to assimilate hydroquinone O-beta-D-glucopyranoside as a sole source of carbon and energy. rirbutin D-arbutin hydroquinone O-beta-D-glucopyranoside arbutin amygdalin assimilation assay amygdaline Assays for the ability of a microorganism to assimilate amygdalin as a sole source of carbon and energy. AMYa amygdalin Carrine Blank amygdalin assimilation D-amygdaline amygladin laetrile D-amygdalin amygdaline assimilation D-galactose assimilation assay Carrine Blank D-galactose Assays for the ability of a microorganism to assimilate D-galactose as a sole source of carbon and energy. dGALa D-galactose assimilation D-galatose Dgalactose Dsalactose Dgalacose gentiobiose assimilation assay gentibiose beta-gentibiose gentiobiose assimilation gentiobiose gentobiose beta-gentiobiose b-gentiobiose beta-gentobiose b-gentibiose Assays for the ability of a microorganism to assimilate gentiobiose as a sole source of carbon and energy. genitobiose GENa b-gentobiose Carrine Blank alpha-lactose assimilation assay D-lactose Carrine Blank a-D-lactose assimilation alactose Dlactose alpha-D-lactose assimilation alpha-D-lactose Assays for the ability of a microorganism to assimilate alpha-lactose as a sole source of carbon and energy. a-D-lactose a-lactose assimilation a-lactose methyl alpha-D-glucopyranoside assimilation assay methyl-a-D-glycoside methyl-alpha-D-glucopyranoside assimilation Assays for the ability of a microorganism to assimilate methyl-alpha-D-glucopyranoside as a sole source of carbon and energy. a-methylglucoside methyl-alpha-D-glucopyranoside methyl-a-Dglucoside a-methyl-D-glycoside a-methyl-D-glucoside a-methyl glucoside methyl-alpha-D-glucoside assimilation methyl-a-D-glucoside assimilation methyl-alpha-D-glucoside methyl-a-D-glucopyranoside assimilation alpha-methyl glucoside methyl-a-D-glucose methyl-a-Dglucopyranoside MAdGa methyl-a-D-glucoside a-methyl-glucoside methyl-aD-glucopyranoside methyl-a-D-glucopyranoside Carrine Blank methyl-a-glucoside beta-cellobiose assimilation assay Assays for the ability of a microorganism to assimilate beta-cellobiose as a sole source of carbon and energy. Dcellobiose dCELa D-cellobiose Carrine Blank Dcellubiose D-celiobiose D-cellobiose assimilation raffinose assimilation assay D-rafnose alpha-raffinose Assays for the ability of a microorganism to assimilate raffinose as a sole source of carbon and energy. raffinose D-raffinose assimilation raflinose rafnose Draffinose raffinose assimilation dRAFa Carrine Blank D-melitose L-raffinose D-raffinose beta-raffinose melezitose assimilation assay D-melezitose assimilation meleziose dMLZa Assays for the ability of a microorganism to assimilate melezitose as a sole source of carbon and energy. D-melezitose melezitose assimilation melezitose melizitose Carrine Blank melizitol Dmelezitose a-melezitose melezitol L-sorbose assimilation assay Carrine Blank L-sorbose Lsorbose Assays for the ability of a microorganism to assimilate L-sorbose as a sole source of carbon and energy. L-sorbose assimilation lSBEa cis-aconitic acid assimilation assay cis-aconitate cis-aconitate assimilation cis-aconitic acid cis-aconitic acid assimilation cisaconitate Carrine Blank Assays for the ability of a microorganism to assimilate cis-aconitate (cis-aconitic acid) as a sole source of carbon and energy. xylitol assimilation assay xylitol assimilation Assays for the ability of a microorganism to assimilate xylitol as a sole source of carbon and energy. Carrine Blank XLTa xylitol D-xylitol turanose assimilation assay turanose assimilation Dturanose D-tutanose Carrine Blank Assays for the ability of a microorganism to assimilate turanose as a sole source of carbon and energy. dTURa turanose D-turanose D-turanose assimilation alpha,alpha-trehalose assimilation assay Carrine Blank alpha,alpha-trehalose dTREa D-tehalose D-trehalose assimilation D-trehalose Assays for the ability of a microorganism to assimilate alpha,alpha-trehalose as a sole source of carbon and energy. Dtrehalose 1-phenylethylamine fermentation assay Carrine Blank 1-phenylethylamine acidification Assays for the ability of a microorganism to ferment 1-phenylethylamine. 1-phenylethylamine 1-phenylethylamine fermentation alpha-D-galacturonic acid assimilation assay D-galacturonate assimilation Assays for the ability of a microorganism to assimilate D-galacturonate (D-galacturonic acid) as a sole source of carbon and energy. Carrine Blank D-galacturonic acid D-galacturonate D-galacturonic acid assimilation L-glutamic acid assimilation assay L-glutamic acid L-glutamate assimilation Assays for the ability of a microorganism to assimilate L-glutamate (L-glutamic acid) as a sole source of carbon and energy. sodium L-glutamate L-glutamate lGLTa Carrine Blank D-xylose assimilation assay D-xyloze D-xylose Dxylose Assays for the ability of a microorganism to assimilate D-xylose as a sole source of carbon and energy. Carrine Blank dXYLa D-xylose assimilation D-glucuronic acid assimilation assay Assays for the ability of a microorganism to assimilate D-glucuronate (D-glucuronic acid) as a sole source of carbon and energy. D-glucuronate Carrine Blank D-glucuronic acid assimilation D-glucuronic acid GRTas D-glucuronate assimilation arginine decarboxylase assay arginine decarboxylase L-arginine decarboxylase Carrine Blank The purpose of this assay is to determine if a microbial isolate is capable of metabolizing arginine under anaerobic conditions. When arginine is metabolized, the pH of the medium increases due to the accumulation of CO2 and organic amines (e.g. agmatine, putrescine). This turns color of the pH indicator (e.g. bromcresol purple, cresol red) to red/orange. A positive test yields a red/orange color; a negative test is yellow. Arginine decarboxylase catalyzes the following reaction: L-arginine <=> agmatine + CO2 Wikipedia:Arginine_decarboxylase glutamyl glycyl arginine arylamidase assay An amino acid arylamidase assay that assays for glutamyl glycyl arginine arylamidase activity. Uses the substrate L-glutamyl-glycyl-L-arginine-2-naphthylamide at pH 7.5. Glutamyl glycyl arginine arylamidase activity (which could be from glutamyl glycyl arginine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. GGAA L-glutamyl-glycyl-L-arginine-b-naphthylamide Glu-Gly-Arg arylamidase L-glutamyl-glycyl-L-arginine-2-naphthylamide Carrine Blank glutamyl glycyl arginine arylamidase glutamyl glycyl arginine aminopeptidase L-glutamyl-glycyl-L-arginine-beta-naphthylamide L-glutamate arylamidase assay glutamate arylamidase An alpha-amino acid arylamidase assay that uses the substrate L-glutamate-2-naphthylamide (L-glutamyl-beta-naphthylamide) at pH 7.5. Glutamate arylamidase activity (which could be from glutamate aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. Test is also performed using L-glutamate-p-nitroanilide. Glutamate arylamidase activity cleaves the substrate, releasing p-nitroaniline which is bright yellow in color. A positive test is yellow; a negative test is colorless. zzzz napthylamide is not nitroanilide AGLTp L-glutamate-beta-naphthylamide L-glutamate-b-naphthylamide L-glutamate arylamidase Carrine Blank L-glutamate 2-naphthylamide L-glutamic acid arylamidase L-glutamate-2-naphthylamide L-glutamyl-2-naphthylamide glutamate aminopeptidase palisade Carrine Blank palisades A multicellular prokaryotic morphological quality where cells so that their long axes are stacked parallel to one another (like in a fence). N-acetyl-beta-galactosaminidase assay Carrine Blank NAGA beta-N-acetyl-galactosaminidase N-acetyl-b-galactosaminidase b-N-acetylgalactosaminidase N-acetyl-beta-galactosaminidase A carbohydrate hydrolysis assay for the activity of N-acetyl-beta-galactosaminidase in a microorganism using various chromogenic substrates. beta-lactamase assay beta-lactamase b-lactamase A carbohydrate hydrolysis assay for the activity of beta-lactamase in a microorganism using various chromogenic substrates. Beta-lactams have at the core of their structure cyclic amides comprised of four-membered ring where one of the atoms is N and the neighboring carbon atom is attached to oxygen via a carbonyl group. Beta lactamase hydrolyzes the beta-lactam ring, resulting in the resistance of the microorganism to beta-lactam antibiotics. Carrine Blank Wikipedia:Beta-lactamase xylanase assay A carbohydrate hydrolysis assay for the activity of xylanase in a microorganism using various chromogenic substrates. Xylanase catalyzes the hydrolysis of beta(1->4) bonds in beta-1,4-xylan polysaccharides (such as hemicellulose), producing xylose (a 5 carbon sugar). Wikipedia:Xylanase xylanase Carrine Blank N-acetyl-beta-galactosaminidase assay with pNP 4-nitrophenyl-N-acetyl-b-D-galactosaminide p-nitrophenyl-N-acetyl-beta-D-galactosaminide para-nitrophenyl-N-acetyl-b-D-galactosaminide pNP-N-acetyl-b-d-galactosaminidase p-nitrophenyl-N-acetyl-b-D-galactosaminide An assay for the activity of N-acetyl-beta-galactosaminidase in a microorganism. Uses the substrate 4-nitrophenyl-N-acetyl-beta-D-galactosaminide (p-nitrophenyl-N-acetyl-beta-D-galactosaminide). N-acetyl-beta-galactosaminidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. para-nitrophenyl-N-acetyl-beta-D-galactosaminide pNP-N-acetyl-b-d-galactosaminidase 1 Carrine Blank 4-nitrophenyl-N-acetyl-beta-D-galactosaminide NAGA1 galactitol assimilation assay dulcit Carrine Blank Assays for the ability of a microorganism to assimilate galactitol as a sole source of carbon and energy. galactitol dulciol dulcitole dulicitol meso-dulcitol dulcitol D-dulcitol dulcitol assimilation dulcital myo-inositol assimilation assay INO inosite myo-inositol Assays for the ability of a microorganism to assimilate myo-inositol as a sole source of carbon and energy. myoinositol m-inositol myo-inositol assimilation i-inositol Carrine Blank meso-inositol myo inositol mesoinositol commercial suite of microbiological diagnostic tests Carrine Blank 6-O-alpha-D-glucopyranosyl-D-fructofuranose assimilation assay PLE isomaltulose Carrine Blank palatinose assimilation palatinose LE Assays for the ability of a microorganism to assimilate 6-O-alpha-D-glucopyranosyl-D-fructofuranose as a sole source of carbon and energy. pyruvic acid assimilation assay Assays for the ability of a microorganism to assimilate pyruvate (pyruvic acid) as a sole source of carbon and energy. pyruvate Carrine Blank pyruvate assimilation sodium pyruvate pyruvic acid PVATE (R)-malic acid fermentation assay dMLT malate fermentation malate acidification malate D-malate fermentation Carrine Blank Assays for the ability of a microorganism to ferment (R)-malate or (R)-malic acid. D-malate acidification D-malic acid (R)-malate (R)-malic acid D-malate maltotriose fermentation assay Carrine Blank maltotriose Assays for the ability of a microorganism to ferment maltotriose. maltotriose fermentation MTE maltotriose assimilation maltotriose assimilation assay MTE Assays for the ability of a microorganism to assimilate maltotriose as a sole source of carbon and energy. maltotriose assimilation maltotriose Carrine Blank L-glutamine assimilation assay Assays for the ability of a microorganism to assimilate L-glutamine as a sole source of carbon, nitrogen, and energy. L-glutamine assimilation L-glutamine lGLM Carrine Blank lecithinase assay http://amrita.vlab.co.in/?sub=3&brch=73&sim=974&cnt=1 lecithinase test lecithinase precipitate is formed on egg-yolk agar An enzymatic assay which tests for the ability of a microorganism to break down lecithin (a generic class fo fatty substances in animal and plant tissues, including phosphrylcholine, fatty acids, glycolipids, triglycerides and phospholipids). Measured by observing a precipitate (an opaque halo) around a microbial colony on egg yolk agar. Carrine Blank precipitate formed on egg yolk agar xylan fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment xylan. oat spelt xylan xylan fermentation XYL xylan xylan acidification beta-glucosidase assay with indoxyl Carrine Blank 5-bromo-4-chloro-3-indoxyl-beta-glucoside An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate 5-bromo-4-chloro-3-indolyl-beta-glucoside. beta-Glucosidase activity will cleave the substrate, producing 5-bromo-4-chloro-3-indoxyl. BGLUi beta-glucuronidase assay with indoxyl BGURi An assay for the activity of beta-glucuronidase in a microorganism. Uses the substrate 5-bromo-4-chloro-3-indoxyl-beta-glucuronide. Beta-glucuronidase will cleave the substrate, producing 5-bromo-4-chloro-3-hydroxyindole, which is oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, which is intensely blue. A positive result is intensely blue; a negative result is colorless. X-GLR Carrine Blank beta-galactosidase assay with indoxyl beta-galactopyranosidase indoxyl BGALi An assay for the activity of beta-galactosidase in a microorganism. An assay for beta-galactosidase activity using X-gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside). Beta-galactosidase will cleave the substrate, producing 5-bromo-4-chloro-3-hydroxyindole, which is oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, which is intensely blue. A positive result is intensely blue; a negative result is colorless. Carrine Blank alpha-galactosidase assay with indoxyl 5-bromo-4-chloro-3-indoxyl-alpha-galactoside Carrine Blank AGALi An assay for the activity of alpha-galactosidase in a microorganism. Uses the substrate 5-bromo-4-chloro-3-indoxyl-alpha-galactoside. Alpha-galactosidase will cleave the substrate, producing 5-bromo-4-chloro-3-hydroxyindole, which is oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, which is intensely blue. A positive result is intensely blue; a negative result is colorless. N-acetyl-beta-glucosaminidase assay with indoxyl 5-bromo-4-chloro-3-indoxyl-beta-N-acetyl-glucosamine BNAGi An assay for the activity of N-acetyl-beta-glucosaminidase in a microorganism. Uses the substrate 5-bromo-4-chloro-3-indoxyl-beta-N-acetyl-glucosamine. N-acetyl-beta-glucosaminidase will cleave the substrate, producing 5-bromo-4-chloro-3-hydroxyindole, which is oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, which is intensely blue. A positive result is intensely blue; a negative result is colorless. Carrine Blank alpha-mannosidase assay with indoxyl 5-bromo-4-chloro-3-indoxyl-alpha-mannoside AMANi An assay for the activity of alpha-mannosidase in a microorganism. An assay for alpha-mannosidase activity using 5-bromo-4-chloro-3-indoxyl-alpha-mannoside. Alpha-mannosidase will cleave the substrate, producing 5-bromo-4-chloro-3-hydroxyindole, which is oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, which is intensely blue. A positive result is intensely blue; a negative result is colorless. Carrine Blank lactic acid assimilation assay DL-lactate D,L-iactate lactate both L- and D-lactate lactic acid lactic acid assimilation D,L-lactate DL-lactate assimilation D- and L-lactic acid DLLactate LATa DL-lactic acid D- or L-lactate D,L-lactic acid lactate assimilation Assays for the ability of a microorganism to assimilate lactate (lactic acid, 2-hydroxypropanoic acid, DL-lactate) as a sole source of carbon and energy. DL-iactate Carrine Blank lactic acids LAT D-fructose assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate D-fructose as a sole source of carbon and energy. D-fructose assimilation D-fructose inulin fermentation assay inulin fermentation inulin chicory inulin D-inulin inuline Carrine Blank Assays for the ability of a microorganism to ferment inulin. inulin acidification INU denitrification assay http://www.microbelibrary.org/library/laboratory-test/3660-nitrate-and-nitrite-reduction-test-protocols Carrine Blank A test or series of tests for enzymes in the denitrification pathway. Nitrate (NO3-) may be reduced to nitrite (NO2-) and then to nitrous oxide (N2O), nitric oxide (NO), nitrogen (N2), or ammonia. nitrite reduction assay nitrite reduction Test for the presence of nitrite reductase in a microbial culture. Nitrite reacts with sulfanilic acid under acidic conditions (5N acetic acid) to produce (colorless) diazotized sulfanilic acid. This sulfanilic acid is then reacted to (colorless) alpha-naphthylamine (or N,N-dimethyl-alpha-naphthylamine) producing a red water soluble azo dye (p-sulfobenzene-azo alpha-naphthylamine). Presence of nitrite then results in a pink/red color (a negative test result). Carrine Blank NO2 nitrogen production from nitrate assay Carrine Blank nitrogen production from nitrate Test for the presence of nitrite reductase in a microbial culture. Complete nitrate reduction to dinitrogen gas will result in the production of gas bubbles. Ellmans assay Carrine Blank Ellman An assay for measuring the presence of low molecular weight thiols (such as glutatione) in liquid solutions, blood, or microbiological cultures. Uses Ellman's reagent (5-5'-dithiobis-(2-nitrobenzoic acid); DTNB) - a chromogenic agent containing a disulfide bond. The substrate reacts with small molecule thiols (e.g. glutathione) and reduce the disulfide bonds, releasing 3-thio-6-nitrobenzoate. At neutral or alkaline pH the product spontaneously ionizes, resulting in a yellow color. A positive result is yellow; a negative result is colorless. ELLM Wikipedia:Ellman's_reagent Ellman's reagent Ellman's assay alpha-L-arabinofuranosidase assay alpha-arabinosidase An L-arabinofuranosidase assay for the activity of alpha-L-arabinofuranosidase in a microorganism using various chromogenic substrates which carries out the hydrolysis of terminal alpha-L-arabinoside residues in alpha-L-arabinosides. Carrine Blank alpha-L-arabinofuranosidase a-arabinosidase alpha-N-arabinofuranosidase Wikipedia:Alpha-N-arabinofuranosidase AARAF phosphonate hydrolase assay Dotson,S.B., Smith,C.E., Ling,C.S., Barry,G.F. and Kishore,G.M. (1996) Identification, characterization, and cloning of a phosphonate monoester hydrolase from Burkholderia caryophilli PG2982. J. Biol. Chem. 271 (42), 25754-25761. phenylphosphonate Carrine Blank Phosphonate hydrolase is an esterase that hydrolyzes a phosphonate compound (an organophosphorus compound with multiple alklyl or aryl groups). Assays for the presence of this enzyme using various chromogenic substrates. OPS cyclodextrin fermentation assay cyclodextrin acidification cyclodextrin Assays for the ability of a microorganism to ferment cyclodextrin (a family of cyclic oligosaccharides) that are intermediates in the breakdown of starch. Carrine Blank cyclo-dextrin CDEX cyclodextrine cyclodextrin fermentation cyclodextrins putrescine assimilation assay putrescine assimilation putrescine Assays for the ability of a microorganism to assimilate putrescine as a sole source of carbon and energy. PSCNa Carrine Blank pyruvic acid fermentation assay pyruvate acidification pyruvate Carrine Blank pyruvic acid Assays for the ability of a microorganism to ferment pyruvate (pyruvic acid). pyruvate fermentation PVATE sodium pyruvate kanamycin resistance assay kanamycin resistance KAN An antibiotic resistance assay for the ability of a microorganism to grow in the presence of kanamycin. Carrine Blank oleandomycin resistance assay An antibiotic resistance assay for the ability of a microorganism to grow in the presence of oleandomycin. Carrine Blank OLD oleandomycin resistance polymixin B resistance assay POLYB_R POLYB An antibiotic resistance assay for the ability of a microorganism to grow in the presence of polymixin B. Carrine Blank polymixin B resistance O/129 resistance assay Carrine Blank O/129 resistance An antibiotic resistance assay for the ability of a microorganism to grow in the presence of O/129 (vibriostatic agent, 2,4-diamino-6,7-diisopropylpteridine). O129R coumaric acid fermentation assay Carrine Blank An assay for the ability of an organism to ferment coumarate (coumaric acid). CMT coumaric acid courmarate phosphatidylinositol phospholipase C assay Carrine Blank As assay for the activity of phosphatidylinositol phospholipase C; an enzyme that catalyzes the hydrolysis of the phosphatidylinositol into inositol triphosphate and diacylglycerol. Assay is made using various chromogenic and fluorogenic substrates. PIPLC bacitracin resistance assay An antibiotic resistance assay for the ability of a microorganism to grow in the presence of bacitracin. BACI Carrine Blank bacitracin sensitivity testing Taxos A optochin resistance assay OPTO Taxos P optochin sensitivity testing Carrine Blank An antibiotic resistance assay for the ability of a microorganism to grow in the presence of optochin (ethylhydrocupreine). methyl beta-D-glucopyranoside fermentation assay methyl-beta-Dglucopyranoside methyl-beta-D-glucoside methyl-beta-D-glucopyranoside 1-O-methyl-b-D-glucopyranoside methyl-beta-glucoside beta-methyl-D-glucoside methyl-b-D-glucopyranoside methyl-b-D-glucopyranoside fermentation Assays for the ability of a microorganism to ferment methyl-beta-D-glucopyranoside. methyl-beta-D-glucose methyl-b-D-glucopyranoside acidification methyl-beta-D-glucopyranoside fermentation Carrine Blank methyl-beta-Dglucoside methyl-D-glucoside methyl-beta-D-glucopyranoside acidification 1-O-methyl-beta-D-glucopyranoside MBdG pullulan fermentation assay PUL Assays for the ability of a microorganism to ferment pullulan (a polysaccharide polymer of maltotriose subunits). pullulan fermentation pullulan acidification Carrine Blank pullan pullulan arginase assay arginine argiamidase ADH2s Amino acid metabolism assay which tests for the presence of arginase (arginine amidinase; canavanase; L-arginase; arginine transamidinase; arginine argiamidase) in a microorganism. When arginine is metabolized, urea is produced. Urea reacts with the colorometric substrates (o-phthalaldehyde and N-(1-naphthyl)ethylenediamine) resulting in a colored pigment whose adsorption is measured at 430 nm. A positive test yields a red/orange color; a negative test is yellow. Arginase catalyzes the reaction: L-arginine + H2O <=> L-ornithine + urea Zawada RJX, Kwan P, Olszewski KL, Llinas M & Huang S-G. 2009. Quantitative determination of urea concentrations in cell culture medium. Biochem Cell Biol 87(3):541-544. ADH1 ARG arginine dihydrolase 2 Carrine Blank BCL ID card Bacillus identification; http://www.biomerieux-usa.com VITEK® is a registered trademark belonging to bioMerieux SA or one of its subsidiaries. Carrine Blank salinity growth assay Carrine Blank An assay for the ability of a microorganism to grow (i.e. undergo cell division) in microbiological culture medium that has various concentrations of sodium chloride (NaCl). growth in 6.5% NaCl assay 6.5 An assay for the ability to grow in the presence of 6.5% sodium chloride (NaCl). Carrine Blank NaCl 6.5% NC6.5 organic acid alkalinization assay Carrine Blank Organic carbon metabolism assay where the ability of a microorganism to increase the pH of the medium as the result of the utlization/metabolism of organic acids (carboxylic acid anions) is assayed. coagulase activity Wikipedia: Coagulase Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. In the laboratory, it is used to distinguish between different types of Staphylococcus isolates. Importantly, S. aureus is generally coagulase-positive, meaning that coagulase negativity usually excludes S. aureus. However it is now known that not all S. aureus are coagulase-positive.[1] [2] It is also produced by Yersinia pestis.[3] Coagulase reacts with prothrombin in the blood. The resulting complex is called staphylothrombin, which enables the enzyme protease to convert fibrinogen, a plasma protein produced by the liver, to fibrin. This results in clotting of the blood. Coagulase is tightly bound to the surface of the bacterium S. aureus and can coat its surface with fibrin upon contact with blood. The fibrin clot may protect the bacterium from phagocytosis and isolate it from other defenses of the host.[4] It has been proposed that fibrin-coated staphylococci resist phagocytosis, making the bacteria more virulent. Bound coagulase is part of the larger family of MSCRAMM. Carrine Blank Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, where the polypeptide chain is prothrombin. succinate alkalinization assay Assays for the ability of a microorganism to assimilate succinate (succinic acid) as a sole source of carbon. Assimilation of succinate results in an increase in pH (alkalinization) as a result of decarboxylation of succinate into propionate and CO2 via succinate decarboxylase. Involves the following enzymes: Succinate-CoA ligase (succinate + CoA + NTP = NDP+ Pi + succinyl-CoA) Methylmalonyl-CoA mutase (succinyl-CoA = methylmalonyl-CoA) Propionyl-CoA carboxylase (methylmalonyl-CoA + Pi + ADP = CO2 + ATP + propanoyl-CoA) Carrine Blank Macy JM, Ljungdahl LG, Gottschalk G. 1978. Pathway of succinate and propionate formation in Bacteroides fragilis. J Bact 134(1):84-91. SUCT succinate alkalinization succinate alkalinisation benzidine assay An assay for the presence of iron-phorphyrin compounds in a microorganism or cell culture. In the presence of hydrogen peroxide and iron-porphyrins, benzidine (4,4'-diaminobiphenyl) forms quinoidic rings (6 membered rings with double bonds) that impart a blue color. Benzidine and other aromatic amine dyes are no longer used because they are carcinogenic. Deibel RH & Evans JB. 1960. Modified benzidine test for the detection of cytochrome-containing respiratory systems in microorganisms. J Bacteroil 79(3):356-360. Carrine Blank bi-polar Gram stain outcome bipolar staining bi-polar Gram stain A phenomenon in Gram staining where the ends of some rods or bacilli stain very little in the center, staining preferentially at the ends of the cells. Bipolar staining is common in enteric Gram-negative bacilli, and can make rods falsely appear to be diplococci. Carrine Blank bi-polar staining chondroitin sulfatase assay chondroitin sulfatase Carrine Blank chondroitinase N-acetylgalactosamine-6-sulfatase test An enzymatic assay which tests for the presence of chondroitin sulfatase (N-acetylgalactosamine-6-sulfatase) in a microorganism. Catalyzes the (hydrolysis) reaction of removing 6-sulfate groups from N-acetyl-D-galactosamine 6-sulfate subunits of the polymer chondroitin sulfate. Also catalyzes the reaction of removing sulfate from D-galactose 6-sulfate groups from keratan sulfate. [chondroitin]-6-O-sulfo-N-acetylgalactosamine + H2O = [chondroitin]-N-acetyl-galactosamine + SO42- + H+ litmus milk assay A milk reactivity assay using to distinguish species of bacteria based on the increase or decrease of pH, protein degradation or denaturation, or lactose fermentation. Contains skim milk powder (which contains lactose and casein) and a pH indictor (litmus). Reactions: Blue color - indicates an alkaline pH (decreased acidity) Red color - indicates acidic pH due to fermentation of lactose (increased acidity) Clearing - indicates peptonization (dissolution of protein due to production of proteases; protein catabolic process) White precipitate (curd formation, clot formation) at the bottom of the tube - indicates milk coagulation (casein denaturation) Gas formation - indicates fermentation resulting in gas production This test yields a complex variation of results, and therefore is not very reliable. Best used as a confirmatory test, not as a diagnostic test. milk is acidified Carrine Blank litmus milk differentiated heteropolar trichome Filament differentiation quality of heteropolar trichomes with respect to the shape of the trichome. Carrine Blank milk coagulation upon boiling assay coagulates immediately on boiling A milk reactivity assay which is used to determine if microorganisms produce acid when grown on milk. Milk (containing whey and casein) does not normally coagulate upon heating or boiling, however if acid (as a result of microbial fermentation) is present the casein will denature and curdle (i.e. coagulate). milk coagulates promptly on boiling Sommer HH, Hart EB. 1919. The heat coagulation of milk. J Biol Chem 40:137-151. From Summary and Conclusions: The main factor in the heat coagulation of fresh milk is the composition of the milk salts. Apparently casein requires a definite optimum calcium content for its maximum stability. the calcium content of casein is largley controlled by the magnesium, citrates, and phosphates present. Acid fermentation in milk lowers the coaguating point by changing the reaction and by lowering the citric acid content. coagulates promptly on boiling Carrine Blank collagenase assay Carrine Blank An assay for the ability of a microorganism to make collagenase. Collagenase ia a protease enzyme that hydrolyzes peptide bonds in collagen (a protein in connective tissues of animals). Are secreted enzymes by some pathogens. L-cysteine arylamidase assay Carrine Blank L-cysteine arylamidase DL-cysteine-beta-naphthylamide cysteine aminopeptidase L-cysteine aminopeptidase L-cysteine-b-naphthylamide L-cysteine-2-naphthylamide cysteine arylamidase An alpha-amino acid arylamidase assay that uses the substrate L-cysteine-2-naphthylamide at pH 7.5. Cysteine arylamidase activity (which could be from cysteine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. phenylalanine deaminase assay phenylalanine deaminase Is an assay for the presence of phenylalanine deaminase activity in a microorganism. Uses phenylalanine agar which contains DL-phenylalanine and ferric chloride. If phenylpyruvic acid is produced, the ferric chloride will turn green. A posivite test is dark green; a negative test is straw in color. L-phenylalanine:2-oxoglutarate aminotransferase activity catalyzes the following: L-phenylalanine + 2-oxoglutarate = phenylpyruvate (phenylpyruvic acid) + L-glutamate phenylalanine dehydrogenase deamination of phenylalanine phenylalanine deamination Carrine Blank cellulase assay A carbohydrate hydrolysis assay for the ability of an organism to utilize/breakdown cellulose. Typically involves synergistic activity of many different types of endo- and exo-cellulases. Carrine Blank endo-cellulase assay An assay for the ability of an organism to break down cellulose, or other beta-D-glucans. Cellulase (endo-1,4-beta-D-glucanose, beta-1,4-glucanse, beta-1,4-endoglucan hydrolase, celludextrinase, endoglucanase D) is a hydrolytic enzyme that catalyzes the breakdown of beta 1,4 linkages in cellulose, licenin, and cereal beta-D-glucans. Carrine Blank EC:3.2.1.4 endo-cellulase activity endo-cellulase exo-cellulase assay 4-methylumbelliferylcellobiopyranoside cellulose 1,4-beta-cellobiosidase Carrine Blank 4-methylumbelliferyl-cellobiopyranoside exo-1,4-beta-D-glucanase 1,4-beta-bellobiohydrolase Test for the presence of exocellulase. Exocellulase is a hydrolytic enzyme that cleaves two or four units from the ends of exposed chains of beta-D-glucan (such as cellulose). Uses 4-methylumbelliferyl beta-D-glucopyranoside as a substrate. Results in the formation of tetra-, or di-saccharides and 4-methylumbelliferone. fucosidase assay A carbohydrate hydrolysis assay for the activity of alpha-fucosidase or beta-fucosidase in a microorganism using various chromogenic substrates. Carrine Blank fucosidase fibrinolysis assay Carrine Blank An assay for the presence of an enzyme in a microorganism that can degrade fibrin ( a protein found in blood clots). fibrinolytic activity Wikipedia: Fibrinolysis Fibrinolysis is a process that prevents blood clots from growing and becoming problematic.[1] This process has two types: primary fibrinolysis and secondary fibrinolysis. The primary type is a normal body process, whereas secondary fibrinolysis is the breakdown of clots due to a medicine, a medical disorder, or some other cause.[1] In fibrinolysis, a fibrin clot, the product of coagulation, is broken down.[2] Its main enzyme plasmin cuts the fibrin mesh at various places, leading to the production of circulating fragments that are cleared by other proteases or by the kidney and liver. fibrinogenolysis assay An assay for the presence of an enzyme in a microorganism that can degrade fibrinogen (a protein which is required for blood clot formation). fibrinogenolytic activity Carrine Blank Wikipedia: Fibrinogenolysis Primary fibrinogenolysis is a medical condition that appears with abnormal production of fibrinogen/fibrin degradation products (FDP), degradation of coagulation factors V, VIII, IX, XI and/or degradation of the fibrin present in any pre-existing localized thrombi and hemostatic clots.[1][2] glutamyl glycine arylamidase assay glutamyl glycine arylamidase glutamyl glycine aminopeptidase An amino acid arylamidase assay that assays for glutamyl glycine arylamidase activity. Uses the substrate L-glutamyl-glycine-2-naphthylamide at pH 7.5. Glutamyl glycine arylamidase activity (which could be from glutamyl glycine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. Carrine Blank glycyl tryptophan arylamidase assay GTA Carrine Blank glycyl tryptophan aminopeptidase glycyl tryptophan arylamidase An amino acid arylamidase assay that assays for glycyl tryptophan arylamidase activity. Uses the substrate glycyl-L-tryptophan-2-naphthylamide at pH 7.5. Glycyl tryptophan arylamidase activity (which could be from glycyl tryptophan aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. glycyl glycine arylamidase assay glycyl-glycyl-2-naphthylamide Carrine Blank glycyl-glycyl-beta-naphthylamide glycyl-glycyl-b-naphthylamide glycyl glycine arylamidase glycyl glycine aminopeptidase An amino acid arylamidase assay that assays for glycyl glycine arylamidase activity. Uses the substrate glycyl-glycine-2-naphthylamide at pH 7.5. Glycyl glycine arylamidase activity (which could be from glycyl glycine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. oxidative-fermentative assay http://www.microbelibrary.org/component/resource/laboratory-test/3151-oxidative-fermentative-test-protocol O/F test Hugh-Leifson test Assay to determine how a microorganism metabolizes glucose, and uses Hugh and Leifson's OF basal medium. Allows for the distinguishing of microorganisms that can ferment glucose anaerobically versus those that metabolize it aerobically (i.e. oxidatively). Organisms that can't metabolize the glucose are consitered to be nonsaccharolytic. The medium contains glucose, agar, with smaller amounts of potassium phosphate, peptone and sodium chloride. It also contains a pH indicator (such as bromthymol blue, which is yellow below pH 6.0 and blue above pH 7.6). The medium is inoculated by stabbing a culture with an inoculating needle half way to the bottom of two test tubes. In one tube, mineral oil is layered onto the agar to prevent oxygen from diffusing down into the culture medium. The culture is incubated. Fermentation of the carbohydrate in the anaerobic tube (and perhaps the aerobic tube) results in a yellow color due to the production of acid. Non-fermenting bacteria can metabolize the glucose aerobically. This is seen as the production of acid in the aerobic tube, but not in the anaerobic tube. Acid production in the aerobic tube should be near the top of the tube. Non-saccharolytic bacteria will result in a negative test - there will be no color change in either tube. In some cases a blue color may be seen in the top layer of the aerobic tube due to an increase in pH caused by the deamination of peptides in the peptone. Instead of glucose, other carbohydrates can be used. Carrine Blank O-F test hyaluronidase assay hyaluronidase hyaluronoglucosaminidase hyaluronoglucosidase chondroitinase Carrine Blank An enzymatic assay which tests for the ability of a microorganism to produce hyaluronidase, and can therefore degrade (hydrolyze) hyaluronic acid (an anionic glycosaminoglycan, found in animal tissues). Assay is performed by adding an acidic albumin solution (pH 3.75 or 4.2). Undegraded hyaluronic acid will become turbid under these conditions, and the turbidity can be measured at 540 or 600 nm using an standard curve of hyaluronic acid that has been dissolved by boiling. EC 3.2.1.35 bathochromic shift assay bathochromic shift KOH test turns red upon addition of 20% KOH Carrine Blank A microbiological diagnostic assay for the presence of flexirubin-type pigments. Upon flooding a colony with 20% KOH, a bathochromic shift (a shift in the absorbance of a compound) occurs resulting in the yellow (or orange) flexirubin pigment turning red/brown/purple. When flooded with an acidic solution, the color will revert back to the original color. Reichenbach, H. 1989. Order I: Cytophagales Leadbetter 1974. In: J. T. Staley, M. P. Bryant, N. Pfennig, and J. G. Holt (Eds.) Bergey’s Manual of Systematic Bacteriology. Williams and Wilkins. Baltimore, MD. 3:2011–2013. lysine iron agar From: Lysine Iron Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Lysine Iron Agar is used for the differentiation of enteric organisms based on their ability to decarboxylate or deaminate lysine and to form hydrogen sulfide. Principles of the Procedure Dextrose serves as a source of fermentable carbohydrate. The pH indicator, bromcresol purple, is changed to a yellow color at or below pH 5.2 and is purple at or above pH 6.8.8 Ferric ammonium citrate and sodium thiosulfate are indicators of hydrogen sulfide formation. Lysine is the substrate for use in detecting the enzymes, lysine decarboxylase and lysine deaminase. Cultures of enteric bacilli that produce hydrogen sulfide cause blackening of the medium due to the production of ferrous sulfides. Those that produce lysine decarboxylase produce an alkaline reaction (purple color) or neutral reaction in the butt of the medium. Organisms that deaminate the lysine cause the development of a red slant over an acid butt. Gas may be formed but its formation is often irregular or suppressed. Formulae Difco™ Lysine Iron Agar Approximate Formula* Per Liter Peptone....................................................................... 5.0 g Yeast Extract................................................................ 3.0 g Dextrose...................................................................... 1.0 g L-Lysine HCl............................................................... 10.0 g Ferric Ammonium Citrate............................................. 0.5 g Sodium Thiosulfate...................................................... 0.04 g Bromcresol Purple........................................................ 0.02 g Agar.......................................................................... 15.0 g pH 6.7 ± 0.2 BBL™ Lysine Iron Agar Approximate Formula* Per Liter Pancreatic Digest of Gelatin......................................... 5.0 g Yeast Extract................................................................ 3.0 g Dextrose...................................................................... 1.0 g L-Lysine...................................................................... 10.0 g Ferric Ammonium Citrate............................................. 0.5 g Sodium Thiosulfate...................................................... 0.04 g Bromcresol Purple........................................................ 0.02 g Agar.......................................................................... 13.5 g pH 6.7 ± 0.2 Carrine Blank An organic-rich, solid microbiological culture medium containing peptones, yeast extract, glucose, L-lysine, ferric iron, thiosulfate, and a pH indicator (bromcresol purple). Lysine decarboxylation (an anaerobic process) that occurrs in the butt of the culture tube. Decarboxylation causes the color indicator (bromcresol purple) to turn purple due to the production of amines at the bottom of the tube. Posivite lysine decarboxylation: purple tube butt; negative reaction: yellow tube butt. Lysine deamination (an aerobic process) results in the production of ammonia throughout the slant, which will result in an overall red color (due to the reaction of ammonia with ferric ammonium citrate). Positive lysine deamination: red throughout the slant; negative reaction: purple slant. Hydrogen sulfide formation (from thiosulfate reduction) results in the presence of a black precipitate. Positive hydrogen sulfide: black precipitate; negative rection: no precipitate. pectinase assay pectinase pectinase test Carrine Blank An enzymatic assay which tests for the presence of pectinase in a microorganism. Pectinase is a hydrolase enzyme that degrades pectin, a polysaccharide found in the cell walls of plants. Pectin is a heteropolysaccharide comprised mostly of galacturonic acid. lysine deaminase assay lysine deaminase It is not clear which enzyme is being referred to as "lysine deaminase"; here is no such enzyme in GenBank. A putative enzyme in MetaCyc include D-lysine oxidase (studied in Pseudomonas), however the homologs in other Enterobacteriaceae have not been defined. In addition, the distribution of putative orthologs of D-lysine oxidase appear to have a different taxonomic distribution than reported lysine deaminase activity. Another putative enzyme is Lysine dehydrogenase, however homologs in the Enterobacteriaceae are only found in the genomes of Photorhabdus and Yersinia. Another putative enzymatic pathway includes the degradation of lysine to 5-amiopentanamide and 5-aminopentanoate via the enzymes L-lysine monooxygenase and delta-aminovaleramidase. However these enzymes appear to be widely spread in the Enterobacteriaceae, including those that are negative for lysine deaminase activity. Carrine Blank Test for the ability of a microorganism to deaminate lysine. Lysine deamination is an aerobic process. malate dehydrogenase assay malate dehydrogenase Carrine Blank An enzymatic assay which tests for the presence of malate dehydrogenase in a microorganism. MDH methyl alpha-D-mannoside assimilation assay methyl-a-Dmannopyranoside methyl a-D-mannoside methyl-D-mannopyranoside a-methyl-D-mannose glycosides methyl alpha-D-mannopyranoside methyl-a-D-mannotpyranside methyl-aD-mannopyranoside Assays for the ability of a microorganism to assimilate methyl alpha-D-mannoside as a sole source of carbon and energy. methyl a-D-mannopyranoside alpha-methylmannoside a-methyl-D-mannose Carrine Blank a-methyl-D-mannopyranside methy a-d-mannopyranoside a-methylmannoside assimilation methyl-alpha-D-mannoside methyl a-D-mannopyranoside assimilation methyl-D-mannoside methyl alpha-D-mannopyranoside assimilation methyl-a-mannoside a-methyl-mannoside alpha-methylmannoside assimilation methyl-a-Dmannoside methyl-a-D-mannose methyl alpha-D-mannoside assimilation methyl a-D-mannoside assimilation a-methyl-D-mannoside methyl-a-D-mannopyranoside a-methylmannoside methyl-a-D-mannoside methyl alpha-D-mannoside methyl beta-D-xylopyranoside assimilation assay methyl-D-xyloside b-methylxyloside Assays for the ability of a microorganism to assimilate methyl beta-D-xylopyranoside as a sole source of carbon and energy. methyl b-D-xyloside assimilation D-methyl-beta-D-xylopyranoside b-methylxyloside assimilation methyl beta-D-xyloside assimilation beta-methylxyloside methyl-D-xylopyranoside methyl b-D-xylopyranoside methyl-beta-D-xylopyrnaoside Carrine Blank methyl-beta-Dxylopyranoside methyl-beta-D-xyloside methyl-beta-D-xylopyranside methyl beta-D-xylopyranoside assimilation beta-methylxyloside assimilation methyl b-D-xylopyranoside assimilation methyl b-D-xyloside methyl beta-D-xyloside methyl beta-D-xylopyranoside methyl-beta-D-xylose methyl-beta-Dxyloside methyl-betaD-xylopyranoside beta-methyl-D-xyloside beta-methyl-D-xylose alpha-amino acid arylamidase assay Assays for the ability of a microorganism to hydrolyze alpha-amino acid substrates coordinated with a beta-naphthylamide (2-naphthylamide) moiety. Arylamidases are classes of enzymes with the ability to hydrolyze amino acid-beta-naphtylamide substrates. Sheahan JP, Eitenmiller RR, & Carpenter JA. 1975. Arylamidase activity of Salmonella species. Appl Microbiol 29(6):726-728. Carrine Blank phosphogluconate dehydrogenase assay An enzymatic assay which tests for the presence of phosphogluconate dehydrogenase in a microorganism. Phosphogluconate dehydrogenase (phosphogluconate 2-dehydrogenase, 6-phosphogluconic dehydrogenase, gluconate 6-phosphate dehydrogenase, 2-keto-6-phosphogluconate reductase) catalyzes the following reaction: 6-phospho-D-gluconate + NAD(P) <=> 6-phospho-2-dehydro-D-gluconate + NAD(P)H + H+ phosphogluconate dehydrogenase Carrine Blank N-acetylglucosamine-6-phosphate deacetylase assay N-acetylglucosamine-6-phosphate deacetylase Carrine Blank N-acetylglucosamine phosphate An enzymatic assay which tests for the presence of N-acetylglucosamine-6-phosphate deacetylase in a microorganism. N-acetylglucosamine-6-phosphate deacetylase catalyzes the following reaction: N-acetyl-D-glucosamine 6-phosphate + H2O <=> D-glucosamine 6-phosphate + acetate pyruvate decarboxylase assay pyruvate decarboxylase An enzymatic assay which tests for the presence of pyruvate decarboxylase in a microorganism. Pyruvate decarboxylase (also known as alpha-carboxylase, pyruvic decarboxylase, alpha-ketoacid carboxylase, 2-oxo-acid carboxy-lyase) catalyzes the following reactions: a 2-oxo acid <=> an aldehyde + CO2 Pyruvate <=> acetaldehyde + CO2 Pyruvate + thiamine diphosphate <=> 2-(alpha-hydroxyethyl)thiamine diphosphate + CO2 Acetaldehde + thiamine diphosphate <=> 2-(alpha-hydroxyethyl)thiamine diphosphate Carrine Blank tetrathionate reductase assay Carrine Blank EC:1.97.1.- TRD TET An assay for the presence of tetrathionate reductase in a microorganism. Tetrathionate reductase carries out the following set of reactions: tetrathionate + a reduced electron acceptor <=> 2 thiosulfate + an oxidized electron acceptor Tetrationate reduction generates acid, and therefore the pH drops. Some tests look for acid production in the presence of tetrathionate. aliphatic thiol non-septate filament coenocytic Carrine Blank Filament septation, where septa separating the individual cells that make up the filament are lacking or are incomplete. non-septate alpha-cyclodextrin assimilation assay a-cyclodextrin acyclodextrin alphacyclodextrin Assays for the ability of a microorganism to assimilate alpha-cyclodextrin as a sole source of carbon and energy. Carrine Blank cyclodextrin assimilation alpha-cyclodextrin assimilation alpha-cyclodextrin cyclodextrin a-cyclodextrin assimilation dextrin assimilation assay dextrin dex trin dextrin assimilation dexrin dextrin crystals Assays for the ability of a microorganism to assimilate dextrin as a sole source of carbon and energy. Carrine Blank polysorbate 40 assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate polysorbate 40 as a sole source of carbon and energy. Tween 40 assimilation polysorbate 40 polysorbate 40 assimilation Tween 40 polysorbate 80 assimilation assay Tween 80 assimilation Tween 80 polysorbate 80 Assays for the ability of a microorganism to assimilate polysorbate 80 as a sole source of carbon and energy. Carrine Blank polysorbate 80 assimilation N-acetyl-beta-D-galactosamine assimilation assay N-acetyl-beta-D-galactosamine N-acetyl-beta-D-galactosamine assimilation Carrine Blank Assays for the ability of a microorganism to assimilate N-acetyl-beta-D-galactosamine as a sole source of carbon and energy. ribitol assimilation assay adonitol adonite ribitol adonitol assimilation Assays for the ability of a microorganism to assimilate ribitol as a sole source of carbon and energy. Carrine Blank ribitol assimilation adoniol D-arabinitol assimilation assay D-arabito D-arabitol assimilation D-arabinitol assimilation Assays for the ability of a microorganism to assimilate D-arabinitol as a sole source of carbon and energy. D-arabinol Darabitol Carrine Blank D-arabitol D-arabinitol lactulose assimilation assay Assays for the ability of a microorganism to assimilate lactulose as a sole source of carbon and energy. lactulose assimilation Carrine Blank lactulose methyl beta-D-glucopyranoside assimilation assay methyl-b-D-glucopyranoside beta-methyl-D-glucoside assimilation methyl-b-D-glucopyranoside assimilation methyl-beta-D-glucoside Carrine Blank b-methyl-D-glucoside methyl-D-glucoside b-methyl-D-glucoside assimilation methyl-beta-D-glucopyranoside assimilation methyl-beta-D-glucose 1-O-methyl-beta-D-glucopyranoside Assays for the ability of a microorganism to assimilate methyl-beta-D-glucopyranoside as a sole source of carbon and energy. methyl-beta-glucoside methyl-beta-Dglucoside methyl-beta-D-glucopyranoside beta-methyl-D-glucoside D-psicose assimilation assay D-psicose D-psicose assimilation Carrine Blank Assays for the ability of a microorganism to assimilate D-psicose as a sole source of carbon and energy. DSMZ Medium 9.1 DSMZ Medium 9.1 -< for DSM 111163 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium9.pdf 9. VY/2 AGAR Baker's yeast 5.00 g CaCl2 x 2 H2O 1.36 g Vitamin B12 0.50 mg Agar (Difco) 15.00 g Distilled water 1000.00 ml Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50˚C (use pH-indicator paper). For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium MD1 (per liter: casiton 3.0 g; calciumchloride dihydrate 0.7 g; magnesiumsulphate heptahydrate 2.0 g) to the vial with freeze dried material. For DSM 11116 reduce amount of vitamin B12 to 0.05 mg/l. © 2007 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 9, except the concentration of cobalamin is decreased. Carrine Blank DSM 11116 is Hymenobacter gelipurpurascens monomethyl succinate assimilation assay succinic acid mono-methyl-ester succinic acid mono-methyl ester assimilation succinic acid mono methyl ester succinic acid mono-methylester Assays for the ability of a microorganism to assimilate monomethyl succinate as a sole source of carbon and energy. Carrine Blank monomethyl succinate monomethyl succinate assimilation succinic acid mono-methyl ester formic acid assimilation assay formic acid assimilation sodium formate formic acid Assays for the ability of a microorganism to assimilate formate (formic acid) as a sole source of carbon and energy. formate assimilation formate Carrine Blank D-galactonolactone assimilation assay D-galactono-1,4-lactone Carrine Blank D-galactonic acid lactone assimilation D-galactonic acid gamma-lactone Assays for the ability of a microorganism to assimilate D-galactono-1,4-lactone or D-galactono-1,5-lactone as a sole source of carbon and energy. D-galactonic acid gamma-lactone assimilation D-galactono-1,4-lactone assimilation D-galactonic acid lactone 2-amino-2-deoxy-D-gluconic acid assimilation assay D-glucosaminic acid D-glucosaminate assimilation glucosaminate 2-amino-2-deoxy-D-gluconic acid glucosaminic acid Assays for the ability of a microorganism to assimilate 2-amino-2-deoxy-D-gluconate (2-amino-2-deoxy-D-gluconic acid) as a sole source of carbon and energy. glucosaminic acid assimilation 2-amino-2-deoxy-D-gluconate Carrine Blank D-glucosaminic acid assimilation D-glucosaminate glucosaminate assimilation 2-hydroxybutyric acid assimilation assay Carrine Blank 2-hydroxybutyrate 2-hydroxybutyrate assimilation Assays for the ability of a microorganism to assimilate 2-hydroxybutyrate (2-hydroxybutyric acid, alpha-hydroxybutyric acid) as a sole source of carbon and energy. 2-hydroxybutyric acid assimilation a-hydroxybutyric acid alpha-hydroxybutyric acid assimilation a-hydroxy-butyric acid a-hydroxybutyrate alpha-hydroxybutyrate 2-hydroxybutyric acid alpha-hydroxybutyric acid ahydroxybutyric acid alpha-hydroxybutyrate assimilation 4-hydroxybutyric acid assimilation assay g-hydroxybutyric acid g-hydroxybutyrate gamma-hydroxybutyrate 4-hydroxybutyrate assimilation gamma-hydroxybutyric acid assimilation gamma-hydroxybutyric acid gamma-hydroxybutyric acids 4-hydroxybutyric acid assimilation 4-hydroxybutyric acid g-hydroxy butyric acid gamma-hydroxybutyrate assimilation 4-hydroxybutyrate Assays for the ability of a microorganism to assimilate 4-hydroxybutyrate (4-hydroxybutyric acid) as a sole source of carbon and energy. Carrine Blank 4-hydroxyphenylacetic acid assimilation assay Assays for the ability of a microorganism to assimilate 4-hydroxyphenylacetate (4-hydroxyphenylacetic acid) as a sole source of carbon and energy. Carrine Blank p-hydroxyphenylacetate assimilation p-hydroxy-phenylacetic acid 4-hydroxyphenylacetic acid assimilation 4-hydroxyphenylacetate 4-hydroxyphenylacetic acid p-hydroxyphenylacetate p-hydroxyphenylacetic acid p-hydroxy-phenylactic acid 4-hydroxyphenylacetate assimilation p-hydroxyphenylacetic acid assimilation 2-oxobutanoic acid assimilation assay a-ketobutyric acid a-ketobutyric acid assimilation Assays for the ability of a microorganism to assimilate 2-oxobutanoate (2-oxobutanoic acid) as a sole source of carbon and energy. alpha-ketobutyric acid assimilation 2-ketobutyric acid assimilation 2-ketobutyrate assimilation a-ketobutyrate assimilation alpha-ketobutyrate a-keto-butyric acid a-ketobutyrate alpha-ketobutyric acid alpha-ketobutyrate assimilation Carrine Blank 2-oxobutanoate 2-oxobutanoic acid 2-ketobutyrate 2-ketobutyric acid 2-oxoglutaric acid assimilation assay alpha-ketoglutaric acid alpha-ketoglutarate assimilation 2-ketoglutaric acid assimilation a-keto-glutaric acid a-ketoglutaric acid 2-oxoglutaric acid 2-oxoglutarate Carrine Blank Assays for the ability of a microorganism to assimilate 2-oxoglutaric acid (2-oxoglutarate) as a sole source of carbon and energy. alpha-ketoglutarate 2-ketoglutaric acid 2-ketoglutarate a-ketoglutarate assimilation a-ketoglutaric acid assimilation 2-ketoglutarate assimilation a-ketoglutarate alpha-ketoglutaric acid assimilation sodium a-ketoglutarate 2-oxopentanoic acid assimilation assay Assays for the ability of a microorganism to assimilate 2-oxopentanoate (2-oxopentanoic acid) as a sole source of carbon and energy. alpha-ketovalerate 2-oxopentanoate assimilation alpha-ketovaleric acid assimilation a-ketovalerate 2-oxopentanoic acid assimilation 2-ketovalerate a-ketovalerate assimilation a-ketovaleric acid assimilation alpha-ketovaleric acid a-ketovaleric acid 2-oxopentanoic acid 2-oxopentanoate alpha-ketovalerate assimilation Carrine Blank quinic acid assimilation assay quinic acid quinic acid assimilation quinate quinate assimilation Assays for the ability of a microorganism to assimilate quinate (quinic acid) as a sole source of carbon and energy. Carrine Blank D-glucaric acid assimilation assay D-glucaric acid assimilation D-saccharic acid Carrine Blank D-glucarate Assays for the ability of a microorganism to assimilate D-glucarate (D-glucaric acid) as a sole source of carbon and energy. D-saccharate D-glucaric acid D-glucarate assimilation sebacic acid assimilation assay Carrine Blank sebacic acid decanedioic acid sebacate 1,8-octanedicarboxylic acid sebacic acid assimilation Assays for the ability of a microorganism to assimilate sebacate (sebacic acid) as a sole source of carbon and energy. sebacate assimilation bromosuccinic acid assimilation assay bromosuccinate Carrine Blank bromosuccinic acid bromosuccinate assimilation bromosuccinic acid assimilation Assays for the ability of a microorganism to assimilate bromosuccinate (bromosuccinic acid) as a sole source of carbon and energy. succinamic acid assimilation assay succinamic acid Carrine Blank succinamate Assays for the ability of a microorganism to assimilate succinamic acid as a sole source of carbon and energy. succinamate assimilation succinamic acid assimilation beta-D-glucuronamide assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate glucuronamide (beta-D-glucuronamide) as a sole source of carbon and energy. glucuronamide beta-D-glucuronamide glucuronamide assimilation L-alaninamide assimilation assay Assays for the ability of a microorganism to assimilate L-alaninamide as a sole source of carbon and energy. alaninamide L-alaninamide L-alaninamide assimilation Carrine Blank alaninamide assimilation D-alanine assimilation assay D-alanine assimilation D-alanine Carrine Blank Assays for the ability of a microorganism to assimilate D-alanine as a sole source of carbon and energy. Ala-Gly assimilation assay Ala-Gly L-alanyl-glycine assimilation L-Ala-Gly L-alanyl-glycine Ala-Gly assimilation alanyl-glycine alanyl glycine assimilation Assays for the ability of a microorganism to assimilate L-alanyl-glycine as a sole source of carbon and energy. Carrine Blank L-asparagine assimilation assay L-asparagine L-asparagine assimilation Carrine Blank Assays for the ability of a microorganism to assimilate L-asparagine as a sole source of carbon and energy. L-aspartic acid assimilation assay L-aspartic acid L-aspartate Assays for the ability of a microorganism to assimilate L-aspartate (L-aspartic acid) as a sole source of carbon and energy. L-asparatate sodium L-aspartate Carrine Blank L-aspartate assimilation L-aspartic acid assimilation Gly-Asp assimilation assay glycyl-L-aspartic acid Gly-Asp assimilation Gly-Asp glycyl-L-aspartic acid assimilation glycyl aspartate assimilation glycyl-L-aspartate assimilation glycyl aspartic acid glycyl aspartate glycyl-L-aspartate glycyl aspartic acid assimilation Carrine Blank Assays for the ability of a microorganism to assimilate glycyl-L-aspartate (glycyl-L-aspartic acid) as a sole source of carbon and energy. Gly-Glu assimilation assay glycyl L-glutamic acid glycyl glutamic acid glycyl glutamate Carrine Blank glycyl glutamate assimilation Assays for the ability of a microorganism to assimilate glycyl-L-glutamate (glycyl-L-glutamic acid) as a sole source of carbon and energy. glycyl-L-glutamic acid assimilation glycyl-L-glutamate assimilation glycyl-L-glutamic acid Gly-Glu assimilation glycyl-L-glutamate Gly-Glu glycyl L-glutamate glycyl glutamic acid assimilation hydroxyproline assimilation assay hydroxyproline L-hydroxyproline hydroxy-L-proline hydroxyproline assimilation L-hydroxyproline assimilation Carrine Blank Assays for the ability of a microorganism to assimilate hydroxyproline as a sole source of carbon and energy. L-leucine assimilation assay Assays for the ability of a microorganism to assimilate L-leucine as a sole source of carbon and energy. Carrine Blank L-leucine L-leucine assimilation L-ornithine assimilation assay L-ornithine Carrine Blank L-ornithine assimilation Assays for the ability of a microorganism to assimilate L-ornithine as a sole source of carbon and energy. L-phenylalanine assimilation assay Carrine Blank L-phenylalanine assimilation Assays for the ability of a microorganism to assimilate L-phenylalanine as a sole source of carbon and energy. L-phenylalanine 5-oxoproline assimilation assay L-pyroglutamate 5-oxoprolinate L-pyroglutamic acid pyroglutamic acid Carrine Blank 5-oxoproline L-pyroglutamate assimilation pyroglutamate L-pyroglutamatic acid assimilation Assays for the ability of a microorganism to assimilate 5-oxoprolinate (5-oxoproline) as a sole source of carbon and energy. pyrrolidonyl D-serine assimilation assay D-serine assimilation Carrine Blank Assays for the ability of a microorganism to assimilate D-serine as a sole source of carbon and energy. D-serine L-threonine assimilation assay L-threonine assimilation Assays for the ability of a microorganism to assimilate L-threonine as a sole source of carbon and energy. L-threonine Carrine Blank carnitine assimilation assay DL-carnitine Carrine Blank D,L-carnitine DL-carnitine assimilation Assays for the ability of a microorganism to assimilate D,L-carnitine as a sole source of carbon and energy. carnitine assimilation carnitine gamma-aminobutyric acid assimilation assay gamma-aminobutyric acid assimilation 4-aminobutyric acid assimilation g-aminobutyrate 4-aminobutyric acid g-aminobutyrate assimilation 4-aminobutyrate assimilation g-aminobutyric acid Carrine Blank 4-aminobutyrate gamma-aminobutyrate Assays for the ability of a microorganism to assimilate gamma-aminobutyrate (gamma-aminobutyric acid) as a sole source of carbon and energy. gamma-aminobutyrate assimilation gamma-aminobutyric acid g-aminobutyric acid assimilation urocanic acid assimilation assay urocanate assimilation urocanate urocanic acid assimilation urocanic acid Carrine Blank Assays for the ability of a microorganism to assimilate urocanate (urocanic acid) as a sole source of carbon and energy. inosine assimilation assay inosine assimilation Assays for the ability of a microorganism to assimilate inosine as a sole source of carbon and energy. inosine Carrine Blank uridine assimilation assay uridine assimilation Carrine Blank uridine Assays for the ability of a microorganism to assimilate uridine as a sole source of carbon and energy. thymidine assimilation assay Assays for the ability of a microorganism to assimilate thymidine as a sole source of carbon and energy. Carrine Blank thymidine thymidine assimilation 1-phenylethylamine assimilation assay Assays for the ability of a microorganism to assimilate 1-phenylethylamine as a sole source of carbon and energy. 1-phenylethylamine phenylethylamine assimilation Carrine Blank 1-phenylethylamine assimilation ethanolamine assimilation assay monoethanolamine assimilation 2-aminoethanol Carrine Blank 2-aminoethanol assimilation Assays for the ability of a microorganism to assimilate ethanolamine as a sole source of carbon and energy. ethanolamine ethanolamine assimilation monoethanolamine butane-2,3-diol assimilation assay butanediol assimilation Assays for the ability of a microorganism to assimilate 2,3-butanediol (butane-2,3-diol) as a sole source of carbon and energy. 2,3-butanediol Carrine Blank 2,3-butanediol assimilation 2,3-buthanediol butanediol glycerol phosphate assimilation assay Assays for the ability of a microorganism to assimilate glycerol phosphate as a sole source of carbon and energy. glycerol phosphate glycerol PO4 assimilation DL-a-glycerol phosphate D,L-a-glycerol phosphate DL-glycerol phosphate glycerol PO4 Carrine Blank D,L-alpha-glycerol phosphate DL-glycerol PO4 glycerol phosphate assimilation alpha-D-glucose 1-phosphate assimilation assay Assays for the ability of a microorganism to assimilate alpha-D-glucose 1-phosphate as a sole source of carbon and energy. a-D-glucose 1-PO4 alpha-D-glucose 1-phosphate Carrine Blank a-D-glucose 1-phosphate alpha-D-glucose 1-phosphate assimilation D-glucose 6-phosphate assimilation assay glucose 6-phosphate assimilation D-glucose 6-phosphate glucose 6-PO4 Assays for the ability of a microorganism to assimilate D-glucose 6-phosphate as a sole source of carbon and energy. D-glucose-6-PO4 assimilation D-glucose 6-phosphate assimilation D-glucose-6-phosphate assimilation D-glucose-6-PO4 Carrine Blank glucose 6-phosphate beta-cyclodextrin assimilation assay beta-cyclodextrin assimilation Assays for the ability of a microorganism to assimilate beta-cyclodextrin as a sole source of carbon and energy. beta-cyclodextrin b-cyclodextrin assimilation b-cyclodextrin Carrine Blank Biolog test kit Biolog, Inc. is a company that designs cell-based phenotypic testing technologies and assays for microorganisms. Carrine Blank Biolog SF-N2 MicroPlate Biolog SF-N2 MicroPlate™ is designed for the metabolic testing of Sporulating and Filamentous (SF) microorganisms such as actinomycetes and fungi. Cells are resuspended in a gel (of gellan gum or carraghenan) to prevent clumping and uneven cell distributions. Cells also do not contain tetrazoleum violet. A positive reading is determined by growth of the organism on a single carbon source as determined by the appearance of turbidity. The tests performed are the same as those on the GN2 plate. Carrine Blank Biolog GN2 MicroPlate Biolog GN2 MicroPlate™ is designed to identify a wide variety of gram-negative bacteria. Tests for the ability of a microorganism to utilize or oxidize compounds from a preselected panel of different carbon sources. Tetrazolium voilet is used as a redox dye to colorimetrically indicate utilization of the carbon sources. The tests performed are the same as those on the Biolog SF-GN2 plate. Carrine Blank inulin assimilation assay Assays for the ability of a microorganism to assimilate inulin as a sole source of carbon and energy. Carrine Blank chicory inulin D-inulin inuline inulin inulin assimilation mannan assimilation assay Carrine Blank mannan assimilation Assays for the ability of a microorganism to assimilate mannan as a sole source of carbon and energy. mannan N-acetyl-D-mannosamine assimilation assay N-acetyl-D-mannosamine Assays for the ability of a microorganism to assimilate N-acetyl-D-mannosamine as a sole source of carbon and energy. N-acetyl-D-manosamine Carrine Blank N-acetyl-D-mannosamine assimilation methyl alpha-D-galactoside assimilation assay alpha-methyl-D-galactoside methyl alpha-D-galactoside Assays for the ability of a microorganism to assimilate methyl alpha-D-galactoside as a sole source of carbon and energy. methyl-a-D-galactoside methyl alpha-D-galactopyranoside assimilation a-methyl-D-galactoside assimilation methyl a-D-galactopyranoside assimilation Carrine Blank methyl-a-galactopyranoside alpha-methyl-D-galactoside assimilation methyl a-D-galactoside assimilation methyl a-D-galactopyranoside methyl alpha-D-galactopyranoside methyl alpha-D-galactoside assimilation a-methyl-D-galactoside methyl a-D-galactoside methyl beta-D-galactoside assimilation assay Assays for the ability of a microorganism to assimilate methyl beta-D-galactoside as a sole source of carbon and energy. beta-methyl-D-galactoside assimilation methyl beta-D-galactopyranoside assimilation methyl b-galctopyranoside Carrine Blank methyl b-D-galactopyranoside methyl b-D-galactoside beta-methyl-D-galactoside methyl beta-D-galactoside methyl b-D-galactopyranoside assimilation b-methyl-D-galactoside assimilation methyl beta-D-galactopyranoside methyl beta-D-galactoside assimilation b-methyl-D-galactoside methyl b-D-galactoside assimilation methyl beta-galctopyranoside 3-O-methyl-D-glucose assimilation assay methyl glucose assimilation Carrine Blank 3-methyl D-glucose 3-O-methyl-D-glucose 3-O-methylglucose assimilation 3-methyl glucose methyl glucose Assays for the ability of a microorganism to assimilate 3-O-methyl-D-glucose as a sole source of carbon and energy. 3-O-methyl-D-glucopyranose 3-methyl-D-glucose 3-methyl-D-glucose assimilation 3-O-methylglucose 3-methyl D-glucopyranoside 3-methyl glucose assimilation sedoheptulosan assimilation assay Assays for the ability of a microorganism to assimilate sedoheptulosan as a sole source of carbon and energy. sedoheptulosan Carrine Blank sedoheptulosan assimilation stachyose assimilation assay Carrine Blank stachyose Assays for the ability of a microorganism to assimilate stachyose as a sole source of carbon and energy. stachyose assimilation D-tagatose assimilation assay D-tagatose Dtagatose D-tagatose assimilation Assays for the ability of a microorganism to assimilate D-tagatose as a sole source of carbon and energy. Carrine Blank lactamide assimilation assay Assays for the ability of a microorganism to assimilate lactamide as a sole source of carbon and energy. lactamide assimilation lactamide Carrine Blank methyl (R)-lactate assimilation assay D-lactic acid methyl ester assimilation Assays for the ability of a microorganism to assimilate methyl (R)-lactate as a sole source of carbon and energy. methyl (R)-lactate D-lactic acid methyl ester D-lactate methyl ester assimilation Carrine Blank D-lactate methyl ester (R)-malic acid assimilation assay D-malic acid Carrine Blank (R)-malate D-malate assimilation Assays for the ability of a microorganism to assimilate (R)-malate(2-) as a sole source of carbon and energy. D-malic acid assimilation D-malate N-acetyl-L-glutamic acid assimilation assay N-acetylglutamate N-acetyl-L-glutamic acid N-acetyl-L-glutamic acid assimilation N-acetylglutamate assimilation Carrine Blank Assays for the ability of a microorganism to assimilate N-acetyl-L-glutamate (N-acetylglutamic acid) as a sole source of carbon and energy. N-acetyl-L-glutamate assimilation N-acetylglutamic acid N-acetylglutamic acid assimilation N-acetyl-L-glutamate adenosine assimilation assay adenosine adenosine assimilation Carrine Blank Assays for the ability of a microorganism to assimilate adenosine as a sole source of carbon and energy. 2'-deoxyadenosine assimilation assay 29-deoxyadenosine 2-deoxyadenosine deoxyadenosine 2'-deoxy adenosine deoxyadenosine utilization dA Assays for the ability of a microorganism to assimilate 2'-deoxyadenosine as a sole source of carbon and energy. deoxyadenosine assimilation Carrine Blank adenosine 5'-monophosphate assimilation assay AMP assimilation adenosine monophosphate adenosine monophosphate assimilation adenosine 5'-monosphosphate AMP Assays for the ability of a microorganism to assimilate adenosine 5'-monophosphate as a sole source of carbon and energy. adenosine 5'-monophosphate assimilation Carrine Blank thymidine 5'-monophosphate assimilation assay thymidine monophosphate assimilation Carrine Blank Assays for the ability of a microorganism to assimilate thymidine 5'-monophosphate as a sole source of carbon and energy. thymidine 5'-monosphosphate thymidine 5'-monophosphate assimilation thymidine monophosphate TMP TMP assimilation UMP assimilation assay uridine 5'-monophosphate assimilation uridine monophosphate assimilation Carrine Blank uridine 5'-monosphosphate UMP uridine monophosphate Assays for the ability of a microorganism to assimilate UMP as a sole source of carbon and energy. UMP assimilation D-fructose 6-phosphate assimilation assay D-fructose 6-phosphate D-fructose-6-PO4 Carrine Blank Assays for the ability of a microorganism to assimilate D-fructose 6-phosphate as a sole source of carbon and energy. D-fructose 6-phosphate assimilation D-fructose-6-PO4 assimilation Biolog SF-P2 MicroPlate Biolog SF-P2 MicroPlate™ is designed for the metabolic testing of Sporulating and Filamentous (SF) microorganisms such as actinomycetes and fungi. Cells are resuspended in a gel (of gellan gum or carraghenan) to prevent clumping and uneven cell distributions. Cells also do not contain tetrazoleum violet. A positive reading is determined by growth of the organism on a single carbon source as determined by the appearance of turbidity. The tests performed are the same as those on the GP2 plate. Carrine Blank Biolog GP2 MicroPlate Carrine Blank Biolog SF-P2 MicroPlate™ is designed to identify a broad range of gram positive bacteria Tests for the ability of a microorganism to utilize or oxidize compounds from a preselected panel of different carbon sources. Tetrazolium voilet is used as a redox dye to colorimetrically indicate utilization of the carbon sources. The tests performed are the same as those on the SF-P2 plate. pH growth assay Carrine Blank An assay for the ability of a microorganism to grow in a microbiological culture medium at distinct pH values. growth at pH 6.0 6.0 Carrine Blank An assay for the ability of a microorganism to grow at pH 6.0. pH 6 growth at pH 5.0 5.0 pH 5 An assay for the ability of a microorganism to grow at pH 5.0. Carrine Blank growth in 1% NaCl assay 1.0 NaCl 1% Carrine Blank 1% NaCl An assay for the ability to grow in the presence of 1.0% sodium chloride (NaCl). growth in 4% NaCl assay 4.0 Carrine Blank 4% NaCl An assay for the ability to grow in the presence of 4.0% sodium chloride (NaCl). growth in 8% NaCl assay 8.0 An assay for the ability to grow in the presence of 8.0% sodium chloride (NaCl). 8% NaCl Carrine Blank N-acetylneuraminic acid assimilation assay N-acetylneuraminate N-acetyl-neuraminic acid assimilation N-acetyl-neuraminate Carrine Blank N-acetylneuraminic acid N-acetylneuraminic acid assimilation Assays for the ability of a microorganism to assimilate N-acetylneuraminic (N-acetylneuraminate) acid as a sole source of carbon and energy. N-acetyl-neuraminic acid N-acetylneuraminate assimilation N-acetyl-neuraminate assimilation fusidic acid resistance assay Carrine Blank An antibiotic resistance assay for the ability of a microorganism to grow in the presence of fusidic acid. fusidic acid D-aspartic acid assimilation assay Assays for the ability of a microorganism to assimilate D-aspartate (D-aspartic acid) as a sole source of carbon and energy. D-aspartic acid D-aspartate D-aspartic acid assimilation Carrine Blank D-aspartate assimilation troleandomycin resistance assay An antibiotic resistance assay for the ability of a microorganism to grow in the presence of troleandomycin. Carrine Blank troleandomycin rifamycin SV resistance assay rifamycin SV Carrine Blank An antibiotic resistance assay for the ability of a microorganism to grow in the presence of rifamycin SV. minocycline resistance assay An antibiotic resistance assay for the ability of a microorganism to grow in the presence of minocycline. Carrine Blank minocycline gelatin assimilation assay gelatin Assays for the ability of a microorganism to assimilate gelatin as a sole source of carbon and energy. Carrine Blank gelatin assimilation gelatine Gly-Pro assimilation assay Gly-Pro assimilation glycyl-L-prolin Gly-Pro glycyl-L-proline assimilation Carrine Blank glycyl-L-proline glycyl proline assimilation glycyl proline Assays for the ability of a microorganism to assimilate glycyl-L-proline as a sole source of carbon and energy. L-arginine assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate L-arginine as a sole source of carbon and energy. L-arginine Largine L-arginine assimilation growth response to chemical assay An assay for the ability of a microorganism to grow in the presence of a chemical (microbiocides, surfactants, inorganic substance, organic substance, or antibiotics). Carrine Blank lincomycin resistance assay An antibiotic resistance assay for the ability of a microorganism to grow in the presence of lincomycin. Carrine Blank guanidine sensitivity assay guanidine HCl guanidine Carrine Blank A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of guanidine (guanidinium), a protein denaturant. guanidinium HCl guanidium organic chemical sensitivity assay Carrine Blank An assay for the ability of a microorganism to grow or utilize sugars in the presence of a potentially growth inhibiting chemical (an antimicrobial compound). Niaproof 4 sensitivity assay Niaproof 4 A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of Niaproof 4 (Tergitol® 4; sodium tetradecyl sulfate), a anionic detergent. Tergitol® 4 Carrine Blank pectin assimilation assay Carrine Blank pectin assimilation Assays for the ability of a microorganism to assimilate pectin as a sole source of carbon and energy. pectin pecin L-galactono-1,4-lactone assimilation assay L-galacutronic acid lactone L-galactono-1,4-lactone galactono-1,4-lactone Assays for the ability of a microorganism to assimilate L-galactono-1,4-lactone as a sole source of carbon and energy. L-galactono-1,4-lactone assimilation Carrine Blank galactaric acid assimilation assay galactarate galactartic acid mucic acid Assays for the ability of a microorganism to assimilate galactaric acid (galactarate) as a sole source of carbon and energy. mucate assimilation mucate galactarate assimilation Carrine Blank galactartic acid assimilation mucic acid assimilation vancomycin resistance assay An antibiotic resistance assay for the ability of a microorganism to grow in the presence of vancomycin. vancomycin Carrine Blank tetrazolium violet reduction assay An assay for the ability of a microorganism to reduce tetrazolium violet, a redox indicator of microbial growth. Tetrazolium violet is a yellow-green color, however, when reduced it forms a purple color. Carrine Blank tetrazolium violet tetrazolium blue reduction assay Carrine Blank tetrazolium blue An assay for the ability of a microorganism to reduce tetrazolium blue, a redox indicator of microbial growth. Tetrazolium blue is colorless, however, when reduced it forms a blue tetrazoleium diformazan. methyl pyruvate assimilation assay methyl pyruvic acid assimilation methylpyruvate pyruvatic acid methyl ester pyruvic acid methylester methyl pyruvate assimilation pyruvic acid methyl ester pyruvic acid methyl ester methyl pyruvate Carrine Blank Assays for the ability of a microorganism to assimilate methyl pyruvate as a sole source of carbon and energy. pyruvic acid methyl ester assimilation methyl pyruvic acid tellurite inhibition assay Carrine Blank tellurite A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of (potassium) tellurite. potassium tellurite acetoacetic acid assimilation assay b-ketoglutaric acid diactic acid acetoacetate Carrine Blank Assays for the ability of a microorganism to assimilate acetoacetate (acetoacetic acid) as a sole source of carbon and energy. 3-oxoglutaric acid beta-ketoglutaric acid acetonedicarboxylic acid acetoacetic acid assimilation acetoacetate assimilation acetoacetic acid lithium chloride inhibition assay Carrine Blank lithium chloride A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of lithium chloride. aztreonam resistance assay aztreonam An antibiotic resistance assay for the ability of a microorganism to grow in the presence of aztreonam. Carrine Blank butyric acid sensitivity assay butyric acid butyrate A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of sodium butyrate (butyric acid). Carrine Blank bromate sensitivity assay A chemcial sensitivity assay for the ability of a microorganism to grow int he presence of (sodium) bromate. Carrine Blank sodium bromate Biolog Gen III MicroPlate Carrine Blank MicroLog 3 Biolog Gen III MicroPlate™ is designed to identify a broad range of gram-negative and gram-positive bacteria. Tests for the ability of a microorganism to utilize or oxidize compounds from a preselected panel of different carbon sources. Tetrazolium voilet is used as a redox dye to colorimetrically indicate utilization of the carbon sources. D-fucose assimilation assay D-fucose Carrine Blank D-fucose assimilation Dfucose Assays for the ability of a microorganism to assimilate D-fucose as a sole source of carbon and energy. lactate sensitivity assay A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of 1% sodium lactate. 1% sodium lactate Carrine Blank D-serine sensitivity assay D-serine A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of D-serine. Carrine Blank Biolog AN MicroPlate Carrine Blank Biolog AN MicroPlate™ is designed to identify a very wide range of anaerobic bacteria. Tests for the ability of a microorganism to utilize or oxidize compounds from a preselected panel of different carbon sources. Tetrazolium voilet is used as a redox dye to colorimetrically indicate utilization of the carbon sources. fumaric acid assimilation assay fumaric acid assimilation Carrine Blank Assays for the ability of a microorganism to assimilate fumaric acid (fumarate) as a sole source of carbon and energy. fumaric acid fumarate assimilation sodium fumarate fumarate glyoxylic acid assimilation assay glyoxylic acid glyoxylic acid assimilation glyoxylate Assays for the ability of a microorganism to assimilate glyoxylic acid (glyoxylate) as a sole source of carbon and energy. Carrine Blank glyoxylate assimilation meso-tartaric acid assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate meso-tartrate (meso-tartaric acid) as a sole source of carbon and energy. meso-tartartic acid tartartic acid tartrate meso-tartrate assimilation meso-tartrate m-tartaric acid tartaric acid assimilation m-tartrate meso-tartaric acid assimilation m-tartrate assimilation tartrate assimilation m-tartaric acid assimilation L-alanyl-L-glutamic acid assimilation assay L-alanyl-L-glutamic acid L-alanyl-L-glutamate assimilation L-alanyl-L-glutamic acid assimilation alanyl glutamate assimilation Ala-Glu assimilation Ala-Glu Assays for the ability of a microorganism to assimilate L-alanyl-L-glutamate (L-alanyl-L-glutamic acid) as a sole source of carbon and energy. alanyl glutamate Carrine Blank L-alanyl-L-glutamate alanyl glutamic acid alanyl glutamic acid assimilation Ala-His assimilation assay Ala-His assimilation alanyl histidine Ala-His Carrine Blank L-alanyl-L-histidine Assays for the ability of a microorganism to assimilate L-alanyl-L-histidine as a sole source of carbon and energy. alanyl histidine assimilation L-alanyl-L-histidine assimilation Ala-Thr assimilation assay alanyl threonine assimilation Ala-Thr assimilation Ala-Thr Carrine Blank L-alanyl-L-threonine alanyl threonine Assays for the ability of a microorganism to assimilate L-alanyl-L-threonine as a sole source of carbon and energy. L-alanyl-L-threonine assimilation Gly-Met assimilation assay Gly-Met assimilation glycyl methionine glycyl-L-methionine Carrine Blank glycyl-L-methionine assimilation Assays for the ability of a microorganism to assimilate glycyl-L-methionine as a sole source of carbon and energy. Gly-Met glycyl methionine assimilation L-methionine assimilation assay Assays for the ability of a microorganism to assimilate L-methionine as a sole source of carbon and energy. L-methionine Carrine Blank L-methionine assimilation L-valine assimilation assay L-valine Carrine Blank L-valine assimilation Assays for the ability of a microorganism to assimilate L-valine as a sole source of carbon and energy. L-valine plus L-aspartate assimilation assay Assays for the ability of a microorganism to assimilate L-valine plus L-aspartate (L-valine plus L-aspartic acid) as a sole source of carbon and energy. L-valine plus L-aspartate assimilation L-valine plus L-aspartic acid assimilation Carrine Blank L-valine plus L-aspartate L-valine plus L-aspartic acid Gly-Gln assimilation assay Carrine Blank glycyl-L-glutamine assimilation glycyl glutamine Gly-Gln Gly-Gln assimilation Assays for the ability of a microorganism to assimilate glycyl-L-glutamine as a sole source of carbon and energy. glycyl-L-glutamine glycyl glutamine assimilation RapID test kit RapID™ microbiogy test kits. Manufactured and sold variously by Innovative Diagnostics Systems Inc., Remel Inc., and now ThermoScientific. Carrine Blank RapID ANA II system RapID™ ANA II System, designed to identify over 90 medically imporant anaerobes. Carrine Blank RapID ANA II RapID NH system RapID™ NH System, designed to identify over 30 taxa including Neisseria, Moraxella, Haemophilus, and related microorganisms. Carrine Blank RapID NH culture pigmentation assay Carrine Blank PIG The optical quality of a liquid culture of clonal prokaryotic organisms, where the culture medium has a distinctive color that is a result of the prokaryotic production of a pigment compound. RapID CB Plus system Carrine Blank RapID™ CB Plus System, designed to identify Corynebacteria. malonate alkalinization assay Schmid M, Berg M, Hilbi H, Dimroth P. 1996. Malonate decarboxylase of Klebsiella pneumoniae catalyses the turnover of acetyl and malonyl thioester residues on a coenzyme-A-like prosthetic group. Eur J Biochem 237(1):221-228. MAL malonate alkalinisation malonate utilization Assays for the ability of a microorganism to assimilate malonate (malonic acid) as a sole source of carbon. Assimilation of malonate results in an increase in pH (alkalinization) as a result of decarboxylation of malonate into acetate and CO2 via malonate decarboxylase. Involves two enzymes: Malonyl-CoA synthetase (malonate + ATP + CoA = malonyl-CoA + AMP + PPi) Malonyl-CoA decarboxylase (malonyl-CoA = acetyl-CoA + CO2) malonate alkalinization Carrine Blank RapID ONE system RapID™ ONE System, designed for the identification of over 70 medically important Enterobacteriaceae. Carrine Blank RapID STR system Carrine Blank RapID™ STR System, designed for the identification of Streptococci and related genera. thiosulfate reduction assay An assay for the presence of thiosulfate disproportionation, followed by reduction, in a microorganism. Thiosulfate reduction (for example; there are several pathways in microorganisms) can involve the following sets of reactions: thiosulfate -> sulfate + H2S + H+ Sulfate, in turn, may be reduced to sulfide, generating more H+ ions. Thiosulfate reduction generates acid, and therefore the pH drops. Some assays look for acid production in the presence of thiosulfate. Carrine Blank N-benzyl-arginine arylamidase assay An amino acid arylamidase assay that uses the substrate N-benzyl-arginine-2-naphthylamide (N-benzyl-arginine-beta-naphthylamide) at pH 7.5. Benzyl-arginine arylamidase activity (which could be from benzyl-arginine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. Carrine Blank BANA N-benzyl-arginine-beta-naphthylamide RapID NF Plus system Carrine Blank RapID™ NF Plus System, designed for the identification of over 70 medically imporant, oxidase-positive, gram-negative bacilli, including Neisseria, Haemophilus, and Vibrio spp. RapID Staph Plus system RapID™ Staph Plus System, designed for the identification of over 40 staphylococci and related genera. Carrine Blank ammonium salt-sugar medium A mineral-salts, liquid microbiological culture medium for the discrimination of sugar utilization (fermentation) in bacilli. Bacilli may not produce enough acids in conventional peptone-containing medium due to the production of ammonium from the deamination of peptides. Thus, ammonium salt sugar medium contains yeast extract. A positive assay for carbohydrate fermentation is yellow due to a low pH; a negative assay is purple. ammonium salt sugars ammonium salt medium ASS From: Snell JJS, Lapage SP. 1971. Comparison of four methods for demonstrating glucose breakdone in bacteria. J Gen Microbiol 68:221-225. "Ammonium salt sugar (ASS) (Smith, Gordon & Clark, 1952). (NH4),HP04, 1 g.; KCl, 0.2 g. ; MgSO4*7H,O, 0.2 g. ; Yeastrel, 0.2 g. ; agar, 12 g. ; bromocresol purple, 4 ml. of a 1 % (w/v) aqueous solution; glucose, 20 ml. of a 50 % (w/v) Seitz filtered solution; distilled water, 1 1. The solids were dissolved in the water by heating, the pH was adjusted to 7.0 and the indicator added. The medium was sterilized at 115˚ for 15 min. and after cooling to 50˚the sterile glucose solution was added." Note: Yeastrel was a brand of yeast extract (no longer manufactured). Carrine Blank ammonium salt-sugar broth growth factor requirement assay Carrine Blank A microbiological diagnostic assay for the requirement of growth factors (hemin, coenzyme I) to support growth by a microorganism. These growth factors are used for the differentiation of Haemophilus and Bordetella species. The growth factors can be added to the culture medium directly, or they can be added using growth factors impregnated into paper discs. X factor disc haemin Carrine Blank X-factor X growth factor Paper disc impregnated with the growth factor haemin (hemin). hemin V factor disc factor V Carrine Blank Paper disc impregnated with the growth factor NAD (nicotinamide adenine dinucleotide). coenzyme I V-factor X + V factor disc Carrine Blank Paper disc impregnated with the growth factors haemin (hemin) and NAD (nicotinamide adenine dinucleotide). hemin and coenzyme I XV discs CAMP assay http://www.microbelibrary.org/component/resource/laboratory-test/3086-camp-test-protocols Carrine Blank A beta-hemolysis assay used to identify Streptococcus agalactiae (Group B) and to differentiate it from Streptococcus pyrogenes (Group A) and other (Group C to Group H) streptococci. Group B streptococci are CAMP positive; Group A and Nongroup B streptococci are CAMP negative. The assay is performed on Trypticase Soy Agar plates with sheep blood. The test/unknown Streptococcus organism is streaked onto TSA plates at right angles to the beta-lysin positive Staphycoccus aureus. In a positive test (CAMP +) outcome, enhanced zones of clearing will be seen around the test organism when it is near the Staph. aureus. This means that there is a synergistic respones between the test organism and the beta-lysin secreted by the Staph. aureus. In a negative test (CAMP - ) outcome, no enhanced zone of clearing will be seen. indole nitrate medium From: Indole Nitrate Medium/Trypticase Nitrate Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Indole Nitrite Medium is used for the identification of microorganisms by means of the nitrate reduction and indole tests. Principles of the Procedure The casein peptone contains tryptophan, which is attacked by certain microorganisms, resulting in the production of indole, detectable by the addition of chemical reagents to 18- to 48-hour cultures. Potassium nitrate serves as the substrate for determining the ability of microorganisms to reduce nitrates to nitrites. Formula BBL™ Indole Nitrite Medium (Trypticase™ Nitrate Broth) Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 20.0 g Disodium Phosphate.................................................... 2.0 g Dextrose...................................................................... 1.0 g Agar............................................................................ 1.0 g Potassium Nitrate......................................................... 1.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.2 ± 0.2 indole nitrite broth nitrate broth An organic-rich, liquid microbiological culture medium containing pancreatic digest of casein, a phosphate buffer, glucose, and nitrate. Used to test for nitrate reduction and production of indole. indole nitrate broth Carrine Blank indole nitrite medium spirit blue agar Carrine Blank An organic-rich, solid microbiological culture medium containing pancreatic digest of casein, yeast extract, the dye Spirit Blue (a dye indicator of lipolysis), and Spirit Blue Lipase Reagent (contains tributyrin, a triglyceride, and polysorbate 80, an emulsifying agent). Microorganisms with lipolytic activity form colonies with halos. From: Spirit Blue Agar / Lipase Reagent (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Spirit Blue Agar is for use with Lipase Reagent or other lipid source for detecting and enumerating lipolytic microorganisms. Principles of the Procedure Spirit Blue Agar contains peptone as a source of carbon, nitrogen, vitamins and minerals. Yeast extract supplies B-complex vitamins which stimulate bacterial growth. Spirit blue is the indicator of lipolysis. Agar is the solidifying agent. Lipase Reagent contains tributyrin, a true fat and the simplest triglyceride occurring in natural fats and oils. It is a good substrate when testing for lipolytic microorganisms because some microorganisms that hydrolyze tributyrin will not hydrolyze other triglycerides or fats containing longer chain fatty acids.2 Formulae Difco™ Spirit Blue Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 10.0 g Yeast Extract ............................................................... 5.0 g Agar.......................................................................... 20.0 g Spirit Blue.................................................................... 0.15 g Difco™ Lipase Reagent A ready-to-use lipid suspension, containing a mixture of tributyrin and polysorbate 80. *Adjusted and/or supplemented as required to meet performance criteria. pH 6.8 ± 0.2 starch hydrolysis assay Carrine Blank starch hydrolysis test An assay for the ability of a microorganism to hydrolyze starch, using Starch Agar, a medium designed to test for the ability to produce alpha-amylase and oligo-1,6-glucosidase. The agar is flooded with iodine after colonies have developed on the plate, turning starch a dark color. Clearing around colonies indicates starch hydrolysis. motility assay An assay to determine whether a microorganism is motile. Organisms are inoculated into a semi-solid medium. Organisms that are motile will migrate away from the initial inoculum. Medium often includes a redox indicator (such as triphenyltetrazolium chloride) which is reduced producing a red dye which helps show the extend of microbial growth. Carrine Blank starch agar From: Starch Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Starch Agar is used for cultivating microorganisms being tested for starch hydrolysis. Principles of the Procedure Beef extract provides the nitrogen, vitamins, carbon and amino acids in Starch Agar. Starch reacts with Gram Iodine to give a blue color. Organisms hydrolyzing starch through amylase production will produce a clearing around the isolate while the remaining medium is blue. Agar is the solidifying agent. Formula Difco™ Starch Agar Approximate Formula* Per Liter Beef Extract.................................................................. 3.0 g Soluble Starch............................................................ 10.0 g Agar.......................................................................... 12.0 g *Adjusted and/or supplemented as required to meet performance criteria. Directions for Preparation from Dehydrated Product 1. Suspend 25 g of the powder in 1 L of purified water. Mix thoroughly. 2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3. Autoclave at 121°C for 15 minutes. 4. Test samples of the finished product. pH 7.5 ± 0.2 Procedure Starch Hydrolysis Test Flood the surface of a 48-hour culture on Starch Agar with Gram Iodine. Expected Results Starch hydrolysis (+) is indicated by a colorless zone surrounding colonies. A blue or purple zone indicates that starch has not been hydrolyzed (-). Carrine Blank An organic-rich, solid microbiological culture medium containing beef extract and starch. Used to cultivate heterotrophic microorganisms and test for the ability to hydrolyze starch. coagulase assay http://www.microbelibrary.org/library/laboratory-test/3220-coagulase-test-protocol Carrine Blank A microbiological assay of the promotion of blood clotting which assays for the ability of a microorganism to secrete the enzyme coagulase which converts fibrinogen to fibrin and causes clotting of blood plasma. slide coagulase assay slide coagulase test slide coagulation test Carrine Blank Coagulase assay performed on a glass slide, is used to identify the presence of coagulase that is bound to the cell wall of the test microorganism. Cells are emulsified into two drops of saline on a slide. A drop of plasma is added to one of the drops and mixed into the cell suspension. Coagulase reacts with fibrinogen in blood plasma, causing the cells to agglutinate or clump together after 10 seconds. The drop with no plasma acts as a negative control. A positive test shows cells clumping; a negative test shows no clumping. Clumping in the negative control is an indication of autoagglutination. tube coagulase assay Coagulase assay performed in a test tube, is used to identify the presence of coagulase that is bound to the cell wall of the test microorganism or is free of the cell. A microbial colony is suspended in EDTA-treated plasma and incubated for 1-2 hours to permit microbial growth in the plasma. Some species of microbes are able to secrete coagulase into the plasma, forming a clot. Clot formation can take up to 24 hours. A negative control tube is performed in the absence of cells. A positive test shows clot formation; a negative test shows no clot formation. tube coagulase test Carrine Blank tube coagulation test Kligler iron agar An organic-rich, solid microbiological culture medium that contains peptones, two sugars (glucose and lactose), ferric iron, thiosulfate, and phenol red (a pH indicator). If the sugars are fermented, the pH indicator will turn red indicating a lowered pH. If hydrogen sulfide is produced, the ferric iron will be reduced to ferrous sulfide, producing a black color. From: Kligler Iron Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Kligler Iron Agar is used for the differentiation of members of the Enterobacteriaceae on the basis of their ability to ferment dextrose and lactose and to liberate sulfides. Principles of the Procedure Kligler Iron Agar, in addition to casein and meat peptones, contains lactose and dextrose which enable the differentiation of species of enteric bacilli due to color changes of the phenol red pH indicator in response to the acid produced during the fermentation of these sugars. The dextrose concentration is only 10% of the lactose concentration. The combination of ferric ammonium citrate and sodium thiosulfate enables the detection of hydrogen sulfide production. Lactose nonfermenters (e.g., Salmonella and Shigella) initially produce a yellow slant due to acid produced by the fermentation of the small amount of dextrose. When the dextrose supply is exhausted in the aerobic environment of the slant, the reaction reverts to alkaline (red slant) due to oxidation of the acids. The reversion does not occur in the anaerobic environment in the butt, which remains acid (yellow butt). Lactose fermenters produce yellow slants and butts because enough acid is produced in the slant to maintain an acid pH under aerobic conditions. Organisms incapable of fermenting either carbohydrate produce red slants and butts. Hydrogen sulfide production is evidenced by a black color either throughout the butt, or in a ring formation near the top of the butt. Gas production (aerogenic reaction) is detected as individual bubbles or by splitting or displacement of the agar. Formula BBL™ Kligler Iron Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 10.0 g Peptic Digest of Animal Tissue.................................... 10.0 g Lactose...................................................................... 10.0 g Dextrose...................................................................... 1.0 g Sodium Chloride.......................................................... 5.0 g Ferric Ammonium Citrate............................................. 0.5 g Sodium Thiosulfate...................................................... 0.5 g Agar.......................................................................... 15.0 g Phenol Red................................................................ 25.0 mg *Adjusted and/or supplemented as required to meet performance criteria. pH 7.4 ± 0.2 Procedure To inoculate, carefully touch the center of an isolated colony on an enteric plated medium with a cool, sterile needle, stab into the medium in the butt of the tube, and then streak back and forth along the surface of the slant. Several colonies from each primary plate should be studied separately, since mixed infections may occur. Incubate tubes with loosened caps for 18-24 hours at 35 ± 2°C in an aerobic atmosphere.To enhance the alkaline condition in the slant, free exchange of air must be permitted through the use of a loose closure. If the tube is tightly closed, an acid reaction (caused solely by dextrose fermentation) will also involve the slant. Expected Results After incubation, record the reaction in the slant and butt, noting gas formation and hydrogen sulfide production. KIA Carrine Blank alpha-L-arabinofuranosidase assay with pNP An assay for the activity of alpha-arabinosidase in a microorganism. Uses the substrate 4-nitrophenyl-alpha-L-arabinofuropyranoside. Alpha-arabinosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. para-nitrophenyl-alpha-L-arabinofuropyranoside p-nitrophenyl-alpha-L-arabinofuropyranoside p-nitrophenyl-a-L-arabinofuropyranoside p-NP-alpha-L-arabinofuropyranoside Carrine Blank 4-nitrophenyl-alpha-L-arabinofuropyranoside para-nitrophenyl-a-L-arabinofuropyranoside p-NP-a-L-arabinofuropyranoside aARA 4-nitrophenyl-a-L-arabinofuropyranoside alpha-L-arabinofuranosidase assay with 4-MU An assay for the activity of alpha-L-arabinofuranosidase in a microorganism. Uses the substrate 4-methylumbelliferyl-alpha-L-arabinofuropyranoside. Alpha-L-arabinofurosidase will cleave the substrate, producing 4-methylumbelliferone which is highly fluorescent. A positive result is highly fluorescent; a negative result is non-fluorescent. 4-methylumbelliferylarabinopyranoside 4-methylumbelliferyl-alpha-L-arabinopyranoside Carrine Blank film assay Carrine Blank film reaction films A microbiological diagnostic assay for the ability of a microbial colony to develop an irridescent film when grown on egg yolk agar. The irridescence is due to the presence of lipase, which causes the breakdown of fats (triglycerides into free fatty acids) in the egg yolk. spot assay spots spot reaction An assay for the ability of a microbial colony to develop crystalline spots, or a fine crystalline deposit, when grown on egg yolk agar. The spots are due to precipitation of calcium and magnesium "soaps" that are usually associated with a positive film reaction (i.e. the relase of fatty acid anions by lipolytic activity that combine with free calcium and magnesium in the medium). Carrine Blank bile sensitivity assay Carrine Blank growth is inhibited on medium containing 20% bile grow on medium containing 20% bile A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of bile (bile salts). grows in the presence of bile salts growth not inhibited in the presence of 20% bile resistant to 20% bile cell staining assay An assay where cells from a culture or a colony are stained with a dye which will differentially stain (i.e. bind to and be retained in) various chemical components of the cell. Carrine Blank Congo Red absorption assay Carrine Blank congo red is adsorbed. congo red is absorbed. Cell staining assay where a test for how the stain Congo Red stains microbial cells or microbial cell components. Congo Red is adsorbed by cellulose and unusual lipopolysaccharide structures. Can be used to stain cells or colonies. Wikipedia:Congo_red citric acid fermentation assay Assays for the ability of a microorganism to ferment citrate (citric acid). citric acid acidification trisodium citrate citric acid citrate acidification fe citrate iron citrate citrate fermentation Carrine Blank ferric citrate sodium citrate titanium citrate citric acid fermentation citrate malonic acid fermentation assay malonate acidification malonic acid fermentation Carrine Blank malonic acid Assays for the ability of a microorganism to ferment malonate (malonic acid). sodium malonate malonic acid acidification malonate fermenation malonate amygdalin fermentation assay laetrile amygdaline D-amygdalin AMY D-amygdaline Assays for the ability of a microorganism to ferment amygdalin. amygladin amygdalin fermentation Carrine Blank amygdalin acidification amygdalin decarboxylase differential medium An organic-rich liquid medium intended to test for the ability of a microorganism to decarboxylate amino acids, through use of pH indicator compounds. Pyridoxal is added because it is an enzymatic co-factor for amino acid decarboxylases. Peptones and beef/yeast extract and dextrose (D-glucose) are added to promote growth. Lysine, ornithine, or arginine are added to test for decarboxylation. From: Decarboxylase Differential Media (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Decarboxylase media are used in the biochemical differentiation of gram-negative enteric bacilli based on the production of arginine dihydrolase and lysine and ornithine decarboxylase. Decarboxylase Medium Base, with added arginine, lysine or ornithine is used for the same purpose. Lysine Decarboxylase Broth is used for differentiating microorganisms based on lysine decarboxylation. Procedure Inoculate the broth media by transferring one or two colonies from the surface of a fresh culture with an inoculating loop or needle and mix to distribute the culture throughout the medium. Overlay the medium in each tube with 1 mL sterile mineral oil. Incubate the tubes with caps tightened at 35 ± 2°C. Examine for growth and decarboxylase reactions after 18-24, 48, 72 and 96 hours before reporting as negative. The medium will become yellow initially, if the dextrose is fermented, and then will gradually turn purple if the decarboxylase or dihydrolase reaction occurs and elevates the pH. Expected Results Compare the color of tubes of media containing the specific amino acids with the color of control tubes of basal media (without amino acid) that have been inoculated with the same isolate. If inoculated control tubes show an alkaline reaction, the test is invalid; i.e., either improperly performed or the test organisms can degrade the peptone sufficiently to produce an alkaline reaction in the absence of a specific amino acid. The medium becomes purple to violet if the reaction is positive (alkaline). A yellow color indicates a negative test; i.e., the organism does not produce the appropriate enzyme. Formulae Difco™ Decarboxylase Base Moeller Approximate Formula* Per Liter Peptone....................................................................... 5.0 g Beef Extract.................................................................. 5.0 g Dextrose...................................................................... 0.5 g Bromcresol Purple........................................................ 0.01 g Cresol Red................................................................... 5.0 mg Pyridoxal...................................................................... 5.0 mg pH 6.0 ± 0.2 BBL™ Moeller Decarboxylase Broth Base Approximate Formula* Per Liter Peptic Digest of Animal Tissue...................................... 5.0 g Beef Extract.................................................................. 5.0 g Dextrose...................................................................... 0.5 g Bromcresol Purple........................................................ 0.01 g Cresol Red................................................................... 5.0 mg Pyridoxal...................................................................... 5.0 mg Difco™ Decarboxylase Medium Base Approximate Formula* Per Liter Peptone....................................................................... 5.0 g Yeast Extract................................................................ 3.0 g Dextrose...................................................................... 1.0 g Bromcresol Purple........................................................ 0.02 g pH 6.8 ± 0.2 Difco™ Lysine Decarboxylase Broth Approximate Formula* Per Liter Peptone....................................................................... 5.0 g Yeast Extract................................................................ 3.0 g Dextrose...................................................................... 1.0 g L-Lysine........................................................................ 5.0 g Bromcresol Purple........................................................ 0.02 g * Adjusted and/or supplemented as required to meet performance criteria. pH 6.8 ± 0.2 Carrine Blank lysine decarboxylase broth Decarboxylase differential medium containing lysine. Designed to determine whether a microorganism has the ability to decarboxylate lysine. Carrine Blank Moeller decarboxylase broth with ornithine Carrine Blank Decarboxylase differential medium containing ornithine. Designed to determine whether a microorganism has the ability to decarboxylate ornithine. Moeller decarboxylase broth with arginine Carrine Blank Decarboxylase differential medium containing arginine. Designed to determine whether a microorganism has the ability to decarboxylate arginine. 4-methoxy leucine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-leucine-4-methoxy-2-naphthylamide (4-methoxy-leucyl-beta-naphthylamide, L-leucine 4-methoxy-beta-naphthylamide). Carrine Blank anaerobic respiration, using nitrate as electron acceptor The process of anaerobic respiration, where nitrate is reduced to nitrite, nitric oxide, nitrous oxide, or dinitrogen. Carrine Blank anaerobic respiration, using nitrite as electron acceptor The process of anaerobic respiration, where nitrite is reduced to nitric oxide, nitrous oxide, or dinitrogen. Carrine Blank lecithovitellin solution Carrine Blank A substance derived from the treatment of chicken egg yolks with a salt solution, followed by filtration. Lecithovitellin Solution (Methods in Microbiology, v. 6, pt. 1, Chapter 1, Routine Biochemical Tests, pg 18): ...."which is made by adding 1 egg yolk to 225 mL of saline buffered with 0.1 M borate buffer at pH 7.2-7.4 with 0.005 M CaCl2; 10 g of a filtration aid such as Hyflo Supercel (Johns Manville Co., London) is added and the mixture shaken for 1 h before filtering twice through Whatman No. 1 papers and finally Seitz-filtering with negative pressure." egg extract Carrine Blank An aqueous (water) extract of whole chicken egg, used in the cultivation of microorganisms. microbiological medium ingredient, derived from animal blood Carrine Blank Undefined mixture of complex organic compounds comprising a microbiological culture medium ingredient derived from the blood or blood plasma from a mammal. Used to support the growth of microorganisms which need blood to grow. rabbit laked blood Carrine Blank Blood medium ingredient comprised of blood from a rabbit (Orychtolagus cuniculus), which has been laked (treated so that the red blood cells have undergone haemolysis). Used to support the growth of microorganisms which need rabbit laked blood to grow. rabbit blood Whole blood from a rabbit (Oryctolagus cuniculus). Used to support the growth of microorganisms which need rabbit blood to grow. Carrine Blank sheep blood Whole blood from a sheep (Ovis aries). Used to support the growth of microorganisms which need sheep's blood to grow. Carrine Blank sheep's blood washed red blood cells Blood medium ingredient comprised of red blood cells from a mammal that have been washed. Used to support the growth of microorganisms which need washed red blood cells to grow. Carrine Blank lysed red blood cells Carrine Blank Blood medium ingredient comprised of the blood of a mammal that have been lysed (undergone the process of cytolysis). Used to support the growth of microorganisms which need lysed red blood cells to grow. blood medium ingredient A microbiological culture medium ingredient derived from the blood from a mammal. Used to support the growth of microorganisms which need blood to grow. Carrine Blank horse blood Carrine Blank Whole blood of a horse (Equus caballus). Used to support the growth of microorganisms which need horse blood to grow. horse serum Serum medium ingredient comprised of blood serum from a horse (Equus caballus). Used to support the growth of microorganisms which need horse blood serum to grow. Carrine Blank serum medium ingredient Carrine Blank A microbiological culture medium ingredient derived from the blood serum from a mammal. Used to support the growth of microorganisms which need blood serum to grow. From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Serum is the clear yellowish fluid obtained when whole blood is separated into its liquid and solid components. Blood is allowed to clot so that the serum separates from the blood cells. The liquid portion released from the clot is called serum and does not contain fibrinogen as the fibrinogen was utilized to form the fibrin threads of the blood clot. Serum usually is inactivated by heating to 56 degrees C. for 30 minutes to eliminate lipases that would cause degradation of lipids and inactivation of complement. laked blood Blood medium ingredient comprised of the blood from a mammal that has been laked (treated so that the red blood cells have undergone haemolysis). Used to support the growth of a microorganisms which needs laked blood to grow. Is an ingredient for blood agar. Carrine Blank From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Laked Blood is defibrinated blood that has been processed with freeze/thaw cycles to hemolyze the red blood cells. It is clear, red, and homogenous and provides many nutrients. It is useful for growing fastidious organisms due to its highly available nutrient contents. porcine serum Carrine Blank Serum medium ingredient comprised of blood serum from a pig (Sus scrofa). Used to support the growth of microorganisms which need porcine serum to grow. microbiological culture medium containing bile A microbiological culture medium that contains bile (fluid produced by the liver) or bile salts (such as deoxycholate). Used for the culture of microorganisms that require bile. Carrine Blank whole milk medium ingredient Carrine Blank Undefined mixtures of complex organic compounds derived from the whole milk of a mammal. Used in the cultivation of microorganisms. whole milk skim milk powder skim milk skimmed milk Difco™ Skim Milk Microbiological medium ingredient, derived from milk. Comprised of skimmed milk which has been dried to a powder. Carrine Blank skim cow's milk non-fat dry milk casein casein Microbiological medium ingredient, derived from milk. Comprised of a heterogeneous mixture of milk proteins (casein) of a mammal. Used in the cultivation of microorganisms. Carrine Blank microbiological medium ingredient, derived from milk Undefined mixtures of complex organic compounds derived from the milk of a mammal. Used in the cultivation of microorganisms. Carrine Blank alpha-amino acid arylamidase activity An aminopeptidase activity which cleaves alpha-amino acid substrates coordinated with a beta-naphthylamide (2-naphthylamide) moiety. Carrine Blank DL-methionine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate DL-methionine-2-naphthylamide (DL-methionyl-beta-naphthylamide). glycine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-glycine-2-naphthylamide. L-alanine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate alanine-2-naphthylamide (L-alanine-beta-naphthylamide). L-arginine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-arginine-2-naphthylamide (L-arginine-beta-naphthylamide). L-asparagine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-asparagine-2-naphthylamide (L-asparaginyl-beta-naphthylamide). Carrine Blank L-aspartate arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-aspartate-2-naphthylamide (L-aspartic acid-beta-naphthylamide). L-cysteine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-cysteine-2-naphthylamide. Carrine Blank L-cystine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-cystine-di-2-naphthylamide. L-alpha-glutamate arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-alpha-glutamate-2-naphthylamide (L-alpha-glutamyl-beta-naphthylamide). Carrine Blank L-glutamate arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-glutamate-2-naphthylamide (L-glutamyl-beta-naphthylamide). L-glutamine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-glutamine-2-naphthylamide. L-histidine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-histidine-2-naphthylamide. Carrine Blank L-hydroxy proline arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-hydroxyproline-2-naphthylamide (L-hydroxyprolyl-beta-naphthylamide). L-isoleucine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-isoleucyl-2-naphthylamide (L-isoleucine-beta-naphthylamide) . Carrine Blank L-leucine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-leucyl-2-naphthylamide (L-leucine-beta-naphthylamide). L-lysine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-lysine-2-naphthylamide (L-lysyl-beta-naphthylamide). Carrine Blank L-methionine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-methionine-2-naphthylamide (L-methionyl-beta-naphthylamide). Carrine Blank L-ornithine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-ornithine-2-naphthylamide (L-ornithyl-beta-naphthylamide). Carrine Blank L-phenylalanine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-phenylalanine-2-naphthylamide. Carrine Blank L-proline arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-proline-2-naphthylamide. Carrine Blank L-pyrrolidonyl arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate pyroglutamic acid-2-naphthylamide (pyroglutamic acid-beta-naphthylamide). Carrine Blank L-serine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-serine-2-naphthylamide. L-threonine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-threonine-2-naphthylamide (L-threonyl-beta-naphthylamide). L-tryptophan arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-tryptophan-2-naphthylamide (L-tryptophyl-beta-naphthylamide). L-tyrosine arylamidase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate L-tyrosine-2-naphthylamide. L-valine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-valyl-2-naphthylamide. Carrine Blank beta-alanine arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate beta-alanine-2-naphthylamide (beta-alanine beta-naphthylamide). Carrine Blank gamma glutamyl transferase activity Carrine Blank An aminopeptidase activity which cleaves the chromogenic substrate gamma-glutamyl-4-methoxy-beta-naphthylamide. L-gamma-glutamate arylamidase activity An aminopeptidase activity which cleaves the chromogenic substrate L-gamma-glutamate-2-naphthylamide (L-gamma-glutamyl-beta-naphthylamide. Carrine Blank lysine deaminase activity It is not clear which enzyme is being referred to as "lysine deaminase"; here is no such enzyme in GenBank. A putative enzyme in MetaCyc include D-lysine oxidase (studied in Pseudomonas), however the homologs in other Enterobacteriaceae have not been defined. In addition, the distribution of putative orthologs of D-lysine oxidase appear to have a different taxonomic distribution than reported lysine deaminase activity. Another putative enzyme is Lysine dehydrogenase, however homologs in the Enterobacteriaceae are only found in the genomes of Photorhabdus and Yersinia. Another putative enzymatic pathway includes the degradation of lysine to 5-amiopentanamide and 5-aminopentanoate via the enzymes L-lysine monooxygenase and delta-aminovaleramidase. However these enzymes appear to be widely spread in the Enterobacteriaceae, including those that are negative for lysine deaminase activity. A set of microbial enzymatic reactions that result in the deamination of lysine. Carrine Blank phenylalanine agar From: Phenylalanine Agar; Ferric Chloride Reagent (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Phenylalanine Agar is used for the differentiation of enteric bacilli on the basis of their ability to produce phenylpyruvic acid by oxidative deamination. Ferric Chloride Reagent is used to visualize the phenylalanine deamination reaction. Principles of the Procedure The phenylalanine serves as the substrate for enzymes which are able to deaminate it to form phenylpyruvic acid. The addition of 3-5 drops of a 10% aqueous ferric chloride solution (or a 12% aqueous ferric chloride solution acidified with 2.5 mL of concentrated HCl per 100 mL of reagent) to the cultures following incubation results in the appearance of a light to deep green color (positive reaction) or no color change (negative reaction). In a positive reaction, any phenylpyruvic acid present will react with the ferric salt in the reagent to give a green color. Formulae Difco™ Phenylalanine Agar Approximate Formula* Per Liter DL-Phenylalanine.......................................................... 2.0 g Yeast Extract................................................................ 3.0 g Sodium Chloride.......................................................... 5.0 g Dipotassium Phosphate................................................ 1.0 g Agar.......................................................................... 12.0 g pH 7.3 ± 0.2 BBL™ Phenylalanine Agar Approximate Formula* Per Liter DL-Phenylalanine.......................................................... 2.0 g Yeast Extract................................................................ 3.0 g Sodium Chloride.......................................................... 5.0 g Sodium Phosphate....................................................... 1.0 g Agar.......................................................................... 12.0 g pH 7.3 ± 0.2 Difco™/BBL™ Ferric Chloride Reagent Droppers Contain 0.5 mL of 10% ferric chloride in aqueous solution. An organic-rich, solid microbiological culture medium containing DL-phenylalanine, yeast extract and sodium chloride. Used to test for the formation of phenylpyruvic acid. Carrine Blank susceptibility to lysis by water Carrine Blank Prokaryotic cell wall lysis susceptibility that defines how susceptible the cell wall is to lysis when the cell is placed in the presence of an environment with an altered chemical composition (plain water). hypotonic solution Carrine Blank Wikipedia: Tonicity Hypotonicity Hypotonic refers to a lesser concentration. In biology, a hypotonic solution has a lower concentration of solutes outside the cell than inside the cell. In an attempt to balance the concentrations of solutes inside and outside the cell, water will rush into the cell, and can cause it to burst.[2] A chemical solution that has a lower concentration of solutes than the concentration inside a cell. inorganic salt solution A solution of an inorganic salt dissolved in water. Carrine Blank differentiated sub-apical cell Filament differentiation quality, relating to the morphology and differentiation of the sub-apical cell in the filament. Carrine Blank medial cell Trichome cell, which is in the medial region of the trichome, which has not undergone differentiation. Carrine Blank trichome cell Trichome part defined by a single individual cell, which may or may not have undergone differentiation, within the trichome. Carrine Blank thermotolerant Temperature optimum quality, growth rates at moderate temperatures (20-40˚C) are higher than growth rates at other temperatures, but will tolerate higher temperatures. Carrine Blank obligately thermophilic Carrine Blank Thermophilic, can only grow at elevated temperatures (40-85˚C). salinity quality Carrine Blank Culture medium quality with respect to the concentration of salt ions (salinity). freshwater salinity 0.05 0.05 Carrine Blank Culture medium salinity quality where the salinity is less than 0.05 % salts. brackish salinity 0.05 3.0 0.05 3.0 Culture medium salinity quality where the salinity is greater than 0.05 % salts and less than 3 % salts. Carrine Blank marine salinity 3.0 5.0 3.0 5.0 Culture medium salinity quality where the salinity is greater than 3 % salts and less than 5 % salts. Carrine Blank hypersaline salinity 5.0 5.0 Carrine Blank Culture medium salinity quality where the salinity is greater than 5 % salts. hyperthermophilic Temperature optimum quality, where growth rates at highly elevated temperatures (>85˚C) are higher than growth rates at lower temperatures. Carrine Blank cell division in one plane Carrine Blank A cell division pattern where the plane of cell division is always parallel to the previous plane of cell division. cell division in two planes Carrine Blank A cell division pattern where the plane of cell division can occur in two planes, both at right angles to one another. cell division in three planes Carrine Blank A cell division pattern where the plane of cell division can occur in three planes, all three being orthogonal (at right angles) to each other. cell division in random planes Carrine Blank A cell division pattern where the plane of cell division can occur in random planes. Haloferax complex agar Michelle A. Allen,Falicia Goh,Stefan Leuko,Akinobu Echigo,Toru Mizuki,Ron Usami,Masahiro Kamekura, Brett A. Neilan and Brendan P. Burns. 2008. Haloferax elongans sp. nov. and Haloferax mucosum sp. nov., isolated from microbial mats from Hamelin Pool, Shark Bay, Australia. IJSEM 28:798-802. A halophilic archaeal medium [containing (g/L): Casamino acids, 7.5; yeast extract, 10.0; trisodium citrate, 3.00; NaCl, 150; KCl, 2.00; MgSO4 . 7H2O, 20.0; MgCl2 . 6H2O, 7.23; CaCl2 . 2H2O, 2.70; FeSO4 . 7H2O, 0.05; MnSO4.H2O, 0.0002; adjusted to pH 7.4; Goh et al., 2006] solidified with agar complex agar medium Carrine Blank A hypersaline, organic-rich, liquid culture medium containing mineral-salts, magnesium sulfate, citrate, casamino acids and yeast extract. Used for the cultivation of Haloferax. NOM-3 medium A hypersaline, liquid microbiological culture medium containing mineral-salts, pyruvate, lactate, formate, acetate, peptone, and yeast extract. Used for the cultivation of Halolamina pelagica and Halopelagius longus. Carrine Blank From: Heng-Lin Cui, Xia Gao, Xin Yang and Xue-Wei Xu. 2011. Halolamina pelagica gen. nov., sp. nov., a new member of the family Halobacteriaceae. IJSEM 61:1617-1621. 1.0 g yeast extract 0.25 g fish peptone 0.25 g sodium pyruvate, 0.25 g sodium formate 0.25 g sodium acetate 0.25 g sodium lactate 5.4 g KCl, 0.3 g K2HPO4, 0.25 g CaCl2, 0.25 g NH4Cl, 26.8 g MgSO4 . 7H2O, 23.0 g MgCl2 . 6H2O and 184.0 g NaCl (adjusted to pH 7.0–7.2 with 1 M NaOH) cellulolytic rumen bacteria vitamin solution From: Scott HW, Dehority BA. 1965. Vitamin requirements of several cellulolytic rumen bacteria. J Bacteriol 89(5):1169-1175. Vitamins listed From Table 1 (mg per 100 mL) Pyridoxine hydrochloride 0.2 Riboflavine 0.2 Thiamine hydrochloride 0.2 Nicotinamide 0.2 Ca-D-pantothenate 0.2 p-Aminobenzoic acid 0.01 Folic acid 0.005 Biotin 0.005 B12 0.0005 Microbiological vitamin solution, used to support the growth of cellulolytic rumen bacteria. Contains pyridoxine hydrochloride, riboflavin, thiamine, nicotinamide, pathothenate, PABA (i.e. 4-aminobenzoic acid), biotin, and vitamin B12 (i.e. cobalalmin). Carrine Blank obligately hyperthermophilic Hyperthermophilic, can only grow at highly elevated temperatures (>85˚C). Carrine Blank psychrophilic Carrine Blank Temperature optimum quality, where growth rates at depressed temperatures (<20˚C) are higher than growth rates at ambient temperatures. obligately psychrophilic Carrine Blank Psychrophilic, can only grow at depressed temperatures (<20˚C). Allens medium Allen's medium Carrine Blank Allen, M. B. (1959). Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Arch Mikrobiol 32, 270– 277. Table 2. Culture medium for Cyanidium caldarium Macroelements (NH4)2SO4, 0.01 M KH2PO4, 0.002 M MgSO4, 0.001 M CaCl2, 0.0005 M H2SO4, 0.001 M Microelements Fe, 4 mg/L Mn 0.5 mg/L B mg/L Zn mg/L Cu mg/L Mo mg/L V mg/L pH brought to 2 with sulfuric acid. A mineral-salts, liquid microbiological culture medium containing sulfuric acid. For the autotrophic growth of Cyanidium. modified Allen medium From: Li-Jun Liu,3 Xiao-Yan You,3 Xu Guo, Shuang-Jiang Liu and Cheng-Ying Jiang. 2011. Metallosphaera cuprina sp. nov., an acidothermophilic, metal-mobilizing archaeon. IJSEM 61:2395-2400. Allen medium modified by the addition of (l-1): 5 g elemental sulfur, 5 mmol K2S4O6, 10mmol Na2S2O3, 13.9 g FeSO4 . 7H2O, 5 g pyrite or 5 g chalcopyrite. K2S4O6, Na2S2O3 or FeSO4 were added as filter sterilized solutions to the sterilized basal Allen medium (121 ˚C for 20 min). A mineral-salts, liquid microbiological culture medium containing sulfuric acid, ferrous sulfate, thiosulfate, elemental sulfur, tetrathionate, and a metal sulfide (pyrite or chalcopyrite). For the autotrophic growth of Cyanidium. Carrine Blank psychrotolerant Temperature optimum quality, growth rates at moderate temperatures (20-40˚C) are higher than growth rates at other temperatures, but will tolerate lower temperatures. Carrine Blank temperature optimum quality A prokaryotic physiological quality relating the optimal temperature of a particular strain of prokaryotes. When these preferred conditions are met, the organism will exhibit cell division. Carrine Blank salinity optimum quality Carrine Blank A prokaryotic quality relating the optimal salinity of a particular strain of prokaryotes. When these preferred conditions are met, the organism will exhibit cell division. YPS-V medium Carrine Blank An organic-rich, liquid microbiological culture medium containing yeast extract and peptone in a PIPES buffered artificial seawater base. Prepared under an atmosphere of dinitrogen. Used for the cultivation of Palaeococcus helgesonii. From: Jan P. Amend, D’Arcy R. Meyer-Dombard, Seema N. Sheth, Natalya Zolotova, Andrea C. Amend. Palaeococcus helgesonii sp. nov., a facultatively anaerobic, hyperthermophilic archaeon from a geothermal well on Vulcano Island, Italy. Arch Microbiol (2003) 179 : 394–401. contains (per l of water): 28.22 g NaCl, 11.53 g MgCl2·6H2O, 1.56 g MgSO4·7H2O, 0.70 g KCl, 0.20 g NaHCO3. 3 g yeast extract, 3 g peptone, 10 ml concentrated N/P solution (containing per l of water: 127.51 g NH4Cl, 60.50 g NaNO3, 4.61 g K2HPO4), 0.5 ml of a 2% resazurin solution as an oxygen indicator, 5 g of PIPES (piperazine-N,N′-bis[2-ethanesulfonic acid]) as pH buffer. The pH was adjusted to 6.5 with 1.0 N NaOH, and the medium was then autoclaved at 121 °C for 20 min. Subsequently, the following filtersterilized (0.2 μm) solutions were added: 10 ml trace element solution/l (containing per l of water: 11.424 g KBr, 1.024 g AlK(SO4)2·12H2O, 0.423 g MnSO4·H2O, 12.0 mg BaCl2·2H2O, 0.5 mg CoCl2·6H2O, 42.4 mg CuSO4·5H2O, 0.5 mg H2WO4, 0.4 mg Na2MoO4·2H2O, 15.8 mg NiCl2·6H2O, 8.5 mg VOSO4·3.5H2O, 36.5 mg ZnSO4·7H2O, 0.4 mg CdSO4·8/3H2O, 1.8 mg PbCrO4, 66.0 mg RbCl, 5.0 mg SrCl2·6H2O), 5 ml/l CaCl2 solution (containing per l of water: 13.25 g CaCl2·2H2O), and 2ml/l Fe-EDTA solution (containing per l of water: 1.54 g FeSO4·7H2O and 2.06 g Na2EDTA). The YPS-V medium was then heated to boiling and degassed under a steady stream of N2. Ten ml of anoxic medium were transferred to acid-washed Balch tubes (25 ml) containing ~0.3 g S˚ (sterilized by heating to 98 °C in an oven for at least 3 days with occasional stirring) under N2. Tubes were capped with butyl-rubber stoppers and Al crimp seals and pressurized with N2 to 3 bar. Prior to inoculation, growth tubes were reduced with 0.15 ml of a 2.5% Na2S solution. halophilic Salinity optimum quality, where growth rates at elevated salt concentrations are higher than growth rates at lower salt concentrations. Carrine Blank obligately halophilic Carrine Blank Halophilic, where growth occurs only at elevated salt concentrations. halotolerant Carrine Blank Salinity optimum quality, where some growth occurs at elevated salinity, but growth is fastest at lower salinity. pressure optimum quality Carrine Blank A prokaryotic physiological quality relating the optimal pressure of a particular strain of prokaryotes. When these preferred conditions are met, the organism will exhibit cell division. Brocks medium From: Brock TD, Brock KM, Belly RT, Weiss RL. 1972. Sulfolobus: A new genus of sulfur-oxidizing bacteria living at low pH and high temperature. Arch Mikrobiol 84:54-68. Culture Media. For most studies a basal salts medium modified from Allen (1959) was used. This medium contains (NH4)2SO4, 1.3g KH2PO4, 0.28 g MgSO4x7H2O, 0.25g CaCl2x2H2O, 0.07 g FeCl3x6H2O, 0.02g MnCl2x4H2O, 1.8 mg Na2B4O7x10H2O, 0.03 mg VOSO4x2H2O, 0.03 mg CoSO4, 0.01 mg The pH was adjusted with 10 N H2SO4 to pH 1 or 2. Organic supplements were added to the basal medim either as dry powder before autoclaving or from 100-fold concentrated sterile stock solutions (for sugars) after autoclaving. Elemental sulfur was sterilized by steaming in the dry state for 3 h on each of 3 successive days and added at a concentration of about 1 g/100 ml to sterile media. Brock's medium A mineral-salts, liquid microbiological culture medium containing sulfuric acid, elemental sulfur, and cobalt sulfate. For the growth of Sulfolobus spp. Carrine Blank obligately mesophilic Carrine Blank Psychrophilic, can only grow at moderate temperatures (20-45˚C). Berkefeld filter assay A prokaryotic size assay which uses a Berkefeld water filter with a particular pore size to determine the size of a prokaryotic cell. Carrine Blank prokaryotic size assay Carrine Blank A microbiological diagnostic assay which uses a water filter with a particular pore size to determine the size of a prokaryotic cell. Berkefeld N filter assay Carrine Blank A Berkefeld filter assay that tests whether a prokaryotic cell is small enough to pass through a Berkefeld filter candle with an intermediate pore size N (for Normal). Pore sizes average 0.45 microns in diameter. Berkefeld V filter assay Carrine Blank A Berkefeld filter assay that tests whether a prokaryotic cell is small enough to pass through a Berkefeld filter candle with a narrow pore size V (for Viel). Pore sizes average 0.38 microns in diameter. This is the most common filter size used to study the filterability of microorganisms. Allens medium supplemented Allen's medium supplemented with glucose and yeast extract Carrine Blank From: Ren-Long Jan, Jeffrey Wu, Shu-Miaw Chaw, Chien-Wei Tsai and Suh-Der Tsen. 1999. A novel species of thermoacidophilic archaeon, Sulfolobus yangmingensis sp. nov. IJSB 49:1809-1816. .....modified Allen's medium supplemented with glucose and yeast extract under aerobic conditions at low pH.... A mineral-salts, liquid microbiological culture medium containing sulfuric acid, glucose, and yeast extract. For the growth of Sulfolobus yangmingensis. modified R2A agar From: Cui H-L et al. 2010. Halosarcina limi sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halosarcina. Int J. Syst. Evol. Microbiol. 60:2462. CM2 Recipe: Per liter: Casamino acids (Difco), 0.5 g yeast extract (Difco), 0.5 g sodium pyruvate, 0.5 g fish peptone, 0.5 g glucose, 5.0 g sodium glutamate, 0.5 g trisodium citrate, 3.0 g KCl, 2.0 g K2HPO4, 0.3 g CaCl2, 0.5 g MgSO4x7H2O, 20 g NaCl, 230.0 g pH 7.0–7.2 A hypersaline, solid microbiological culture medium containing mineral-salts, magnesium sulfate, pyruvate, glucose, glutamate, citrate, and peptones. Used for the cultivation of Halogranum rubrum. Carrine Blank MR2A agar MH agar A hypersaline, organic-rich, solid microbiological culture medium containing mineral-salts, magnesium chloride and magnesium sulfate, sodium bromide, and yeast extract. Used for the cultivation of Halorubrum aquaticum. Carrine Blank From: M. C. Gutierrez, A. M. Castillo, P. Corral, M. Kamekura and A. Ventosa. 2011. Halorubrum aquaticum sp. nov., an archaeon isolated from hypersaline lakes. IJSEM 61:1144-1148. Water samples were plated on agar plates of halophilic medium (MH), containing (per litre distilled water) 195 g NaCl, 32.5 g MgCl2 . 6H2O, 50.8 g MgSO4 . 7H2O, 0.8 g CaCl2, 5 g KCl, 0.16 g NaHCO3, 0.6 g NaBr, 5 g yeast extract 20 g agar (pH 7.5) modified LPBM basal medium From: SUSUMU ASAKAWA, HIROYUKI MORII, MASAYO AKAGAWA-MATSUSHITA, YOSUKE KOGA, AND KOICHI HAYANO. 1993. Characterization of Methanobrevibacter arboriphilicus SA Isolated from a Paddy Field Soil and DNA-DNA Hybridization among M. arboriphilicus strains. IJSB 43(4):683-686. The basal medium used for enrichment and isolation of strain SA was a modification of LPBM (22), which contained (per liter of deionized water) 0.75 g of KH2P04, 0.75 g of K2HP04, 1.0 g of NH4Cl, 0.36 g of MgCl2.6H20, 9 ml of a trace mineral solution (13), 10 ml of a vitamin mixture solution (2), 0.5 ml of a 0.2% resazurin solution, 0.5 g of L-cysteine hydrochloride H2O, 0.5 g of Na2S.9H20, and 4.8 g of NaHCO3. For cultivation of Methanobrevibacter arboriphilicus strains, the basal medium was supplemented with 0.1% yeast extract (Difco). For roll tubes, we added 0.1% yeast extract (Difco), 0.1% Polypeptone (Daigo Eiyo Co., Osaka, Japan), and 1.7% agar to the basal medium. The gas phase was H2-C02 (4:l) pressurized to 203 kPa, and the pH was 7.0. ******* From: Hiroyuki Morii, Masateru Nishihara and Yosuke Koga. 1983. Isolation, characterization and physiology of a new formate-assimilable methanogenic strain (A2) of Methanobrevibacter arboriphilus. Agric. Biol. Chem. 47(12):2781-2789. The trace mineral solution was as described by Zeikus(4) except that 0.03 g/liter NiCl2 was included. An organic-rich, liquid, anaerobic microbiological culture medium containing L-cysteine, mineral-salts, yeast extract, resazurin, sulfide, sodium bicarbonate, carbon dioxide, and H2 gas as a substrate for methanogenesis. Used for the cultivation of Methanobrevibacter arboriphilicus. Modified by the addition of nickel (in the trace element solution). Carrine Blank Zeikus trace mineral solution A trace elements solution containing potassium hydroxide, nitrilotriacetic acid, ferrous chloride, manganese chloride, cobalt chloride, zinc chloride, calcium chloride, boric acid, and sodium molybdate. From: Zeikus JG. 1977. The biology of methanogenic bacteria. Bacteriol Rev 41:514. Table 4 Trace mineral solution Contains, in grams per liter of distilled water (pH to 7.0 with KOH): nitrilotriacetic acid, 4.5; FeCl2 *4H20, 0.4; MnCl2 * 4H20, 0.1; CoCl2 * 6H2O, 0.17; ZnCl2, 0.1; CaC12, 0.02; H3BO3, 0.019; and sodium molybdate, 0.01. Carrine Blank Berkefeld W filter assay A Berkefeld filter assay that tests whether a prokaryotic cell is small enough to pass through a Berkefeld filter candle with a wider pore size W (for Wenig). Pore sizes average 0.43 microns in diameter. Carrine Blank LPBM basal medium From: Zeikus JG. 1977. The biology of methanogenic bacteria. Bacteriol Rev 41:514. Table 4 Composition of basal medium (LPBM) used for selective enrichment and growth of some methanogenic species Component Amount KH2PO4 ........... ........... 0.75 g K2HPO4-3H2O ...................... 1.45 g NH4Cl ...................... 0.9 g MgCl2 H20 ................ ...... 0.2 g Na2S.9H2Ob ...................... 0.5 g Trace mineral solutionc ................. 9 ml Vitamin solutiond ................... 5 ml Resazurin solution (0.2%)e .............. 1 ml Distilled H20 ............ ....... 1,000 ml a Prepared anaerobically under a 95% N2-5% CO2 gas atmosphere. Medium adjusted to pH 7.4 prior to autoclaving. Basal medium requires the addition of an electron donor (H2, formate, or methanol) for enrichment or growth of methanogens. b Sulfide solution added after sterilization. e Contains, in grams per liter of distilled water (pH to 7.0 with KOH): nitrilotriacetic acid, 4.5; FeCl2 *4H20, 0.4; MnCl2 * 4H20, 0.1; CoCl2 * 6H2O, 0.17; ZnCl2, 0.1; CaC12, 0.02; H3BO3, 0.019; and sodium molybdate, 0.01. d Optional, some methanogens require a vitamin mixture (11, 113). e Optional, an oxidation-reduction indicator. ref 113: Wolin EA, Wolin MJ, Wolfe RS. 1963. Formation of methane by bacterial extracts. J Biol Chem 238(8):2882-2886. Carrine Blank An organic-rich, liquid, anaerobic microbiological culture medium containing mineral-salts, resazurin, sulfide, carbon dioxide, and either formate, methanol or H2 gas as a substrate for methanogenesis. Prepared under an atmosphere of dinitrogen and carbon dioxide. Used for the cultivation of Methanobrevibacter arboriphilicus. corn kernel Carrine Blank Plant embryo of corn (kernel). Halobacter agar A hypersaline, organic-rich, solid microbiological culture medium containing mineral-salts, magnesium sulfate, yeast extract and casamino acids, and sodium glutamate. Used to culture Halobacter spp. Halobacter medium Carrine Blank From ATCC Medium: 2170 Halobacter Medium Yeast Extract……………………………….5.0 g Casamino acids…………………………...5.0 g Sodium Glutamate………………………..1.0 g KCl………………………………………….2.0 g Sodium citrate (tri-Na)…………………….3.0 g MgSO4 x 7H2O…………………………...20.0 g NaCl………………………………………..200.0 g FeCl2 x 4H2O…………………………….36.0 mg MnCl2 x 4H2O……………………………0.36 mg Agar……………………………………….20.0 g DI Water…………………………………..1000 ml Adjust pH to 7.0-7.2. Autoclave at 121ºC. Caution: Due to high salt concentration, agar may not gel firmly. If this happens, agar and salt may have to be sterilized separately and combined. ATCC Medium 2170 Zeikus trace mineral solution with nickel Zeikus trace mineral solution with nickel chloride added. Carrine Blank acetate agar medium A mineral-salts, liquid microbiological culture medium containing resazurin and acetate as a carbon source. Prepared under an atmosphere of carbon dioxide and dinitrogen. For the growth of Methanosaeta (formerly Methanothrix). Carrine Blank From: S. H. Zinder, T. Anguish, and A. L. Lobo. 1987. Isolation and characterization of a thermophilic acetotrophic strain of Methanothrix. Arch Microbiol 146:315-322. Unless stated otherwise, the culture medium contained (g/l): NH4C1, 0.5; K2HPO4, 0.4; MgCI2x 6H2O, 0.1; resazurin, 0.001 ; trace metal solution (Zeikus 1977 except that 0.02 g/1 NiCI2x6H2O was added), 10 ml/1. The medium was either boiled under N2 (scrubbed of O2 by hot copper coils) or bubbled 20 min with N2 to make it anoxic, and was transferred into an anaerobic glove box (Coy Laboratory Products, Ann Arbor, MI, USA) where it was dispensed into 120 ml serum vials which were sealed with aluminum-crimp butyl rubber stoppers (Balch et al. 1979) purchased from Bellco Glass, Inc., Vineland, N J, USA. The vials were autoclaved at 121˚C for 20 minutes. The vial headspaces were flushed with sterile O2-scrubbed 70% N2/30% CO2 (Matheson Gas Products, Peoria, IL, USA) after which N2-flushed syringes were used to add the following sterile anoxic solutions to the following final concentrations: Na2S-9H20, 0.25 g/1 (ca. 1 mM); 2-mercaptoethane sulfonic acid, sodium salt (Sigma Chemical Corp., St. Louis, MO, USA), 1 mM; NaHCO3, 12 mM; CaCl2x2H2O, 0.1 g/l; sodium acetate, 40 mM; vitamin solution (Balch et al. 1979), 0.5ml/50ml. Cultures were routinely incubated at 60˚C, and the pH of the medium at 60˚C was 6.5. Bolds basal medium A mineral-salts, liquid microbiological culture medium. Used to support the autotrophic growth of Cyanobacteria. pH of the medium is 6.6 according to Algal Culturing Techniques, p. 437, copyright 2005. http://www-cyanosite.bio.purdue.edu/media/table/bb.html NaNO3 (5.0 g/200 ml) 10.0 ml (~ 0.25 g) MgSO4·7H2O (1.5 g/200 ml) 10.0 ml (~ 0.75 g) NaCl (0.5 g/200 ml) 10.0 ml (~ 0.025 g) K2HPO4 (1.5 g/200 ml) 10.0 ml (~ 0.075 g) KH2PO4 (3.5 g/200 ml) 10.0 ml (~ 0.175 g) CaCl2·2H2O (0.5 g/200 ml) 10.0 ml (~ 0.025g) H3BO3 (1.14 g/100 ml) 1.0 ml (~ 0.114g) Trace elements solution 1.0 ml EDTA stock 1.0 ml Fe solution 1.0 ml Distilled water to 1.0 L For agar, add 15.0 g/L Bacteriological Agar* (Oxoid L11). Autoclave at 15 psi for 15 minutes. *Supplier: Unipath Ltd, Wade Road, Basingstoke, Hants RG24 0PW, UK Trace elements solution: ZnSO4·7H2O 8.82 g MnCl2·4H2O 1.44 g MoO3 0.71 g CuSO4·5H2O 1.57 g Co(NO3)2·6H2O 0.49 g Distilled water to 1.0 L May need autoclaving to dissolve. EDTA stock: EDTANa2 5.0 g KOH 3.1 g Distilled water to 100 ml Fe solution: FeSO4·7H2O 4.98 g conc H2SO4 1.0 ml Distilled water to 1.0 L Carrine Blank Bold's basal medium Z-8 medium According to SCCAP the medium should have a final pH of 6-7. http://www.sccap.dk/media/freshwater/7.asp A dilute, mineral-salts, liquid microbiological medium comprised of magnesium sulfate, sodium nitrate, calcium nitrate, ammonium chloride, sodium carbonate, ferric chloride, disodium EDTA, and micronutrients. Used for the cultivation of autotrophic Cyanobacteria. Carrine Blank http://www-cyanosite.bio.purdue.edu/media/table/Z8.html MgSO4·7H2O 0.25 g NaNO3 0.467 g Ca(NO3)2·4H2O 59 mg NH4Cl 31 mg Na2CO3 0.02 g FeEDTA solution 10 ml Gaffron micronutrients 1.0 ml Deionized water to 1.0 L FeEDTA solution: Made in two solutions: Solution A - 2.8 g FeCl3 in 100 ml 0.1 N HCl Solution B - 3.9 g EDTANa2 in 100 ml 0.1 N NaOH Add 10 ml solution A and 9.5 ml solution B plus water to 1 L. Gaffron micronutrients: H3BO3 3.1 g MnSO4·4H2O 2.23 g ZnSO4·7H2O 0.22 g (NH4)6Mo7O24·4H2O 0.088 g Co(NO3)2·6H2O 0.146 g VOSO4·6H2O 0.054 g Al2(SO4)3K2SO4·2H2O 0.474 g NiSO4(NH4)2SO4·6H2O 0.198 g Cd(NO3)2·4H2O 0.154 g Cr(NO3)3·7H2O 0.037 g Na2WO4·2H2O 0.033 g KBr 0.119 g KI 0.083 g Deionized water to 1 L olive fruit The fruit of the olive tree (an olive). Carrine Blank Gaffron micronutrient solution Carrine Blank http://www-cyanosite.bio.purdue.edu/media/table/Z8.html Gaffron micronutrients: H3BO3 3.1 g MnSO4·4H2O 2.23 g ZnSO4·7H2O 0.22 g (NH4)6Mo7O24·4H2O 0.088 g Co(NO3)2·6H2O 0.146 g VOSO4·6H2O 0.054 g Al2(SO4)3K2SO4·2H2O 0.474 g NiSO4(NH4)2SO4·6H2O 0.198 g Cd(NO3)2·4H2O 0.154 g Cr(NO3)3·7H2O 0.037 g Na2WO4·2H2O 0.033 g KBr 0.119 g KI 0.083 g Deionized water to 1 L A trace elements solution containing boric acid, manganese sulfate, zinc sulfate, ammonium molybdate, cobalt nitrate, vanadyl sulfate, nickel ammonium sulfate, cadmium nitrate, chromium nitrate, sodium tungstate, potassium bromide, and potassium iodide. soy bean seed The seed of a soy bean. Carrine Blank intracellular storage of phosphorus Carrine Blank Single-organism metabolic process, where the prokaryotic microorganism uses enzymes to synthesize a storage molecule (usually a polymer) inside the cell that is used to store phosphorus. prokaryote specialized with respect to pressure Carrine Blank Prokaryotic physiologically differentiated cell, where the cell is specialized with resect to its optimal growth pressure. prokaryote specialized with respect to salinity Carrine Blank Prokaryotic physiologically differentiated cell, where the cell is specialized with resect to its optimal growth salinity. prokaryote specialized with respect to temperature Carrine Blank Prokaryotic physiologically differentiated cell, where the cell is specialized with resect to its optimal growth temperature. TSBA100 agar http://www.nccs.res.in/mcc/Medium_64a.html TSBA100 TSB (Medium No. 64) 1.0 ml Distilled water 100 ml Agar 1.50 g Carrine Blank Tryptic soy broth diluted 100 times with water, solidified with agar added. TSBA100 TSB100 medium http://www.nccs.res.in/mcc/Medium_64a.html TSB100 TSB (Medium No. 64) 1.0 ml Distilled water 100 ml Carrine Blank Tryptic soy both diluted 100 times with water. BG 11 medium Carrine Blank A liquid, mineral-salts microbiological culture medium. Used to support the autotrophic growth of cyanobacteria. http://www-cyanosite.bio.purdue.edu/media/table/BG11.html NaNO3 1.5 g K2HPO4 0.04 g MgSO4·7H2O 0.075 g CaCl2·2H2O 0.036 g Citric acid 0.006 g Ferric ammonium citrate 0.006 g EDTA (disodium salt) 0.001 g Na2CO3 0.02 g Trace metal mix A5 1.0 ml Agar (if needed) 10.0 g Distilled water 1.0 L The pH should be 7.1 after sterilization Trace metal mix A5: H3BO3 2.86 g MnCl2·4H2O 1.81 g ZnSO4·7H2O 0.222 g NaMoO4·2H2O 0.39 g CuSO4·5H2O 0.079 g Co(NO3)2·6H2O 49.4 mg Distilled water 1.0 L trace metal mix A5 A trace elements solution containing boric acid, manganese chloride, zinc sulfate, sodium molybdate, copper sulfate, and cobalt nitrate. Carrine Blank http://www-cyanosite.bio.purdue.edu/media/table/BG11.html Trace metal mix A5: H3BO3 2.86 g MnCl2·4H2O 1.81 g ZnSO4·7H2O 0.222 g NaMoO4·2H2O 0.39 g CuSO4·5H2O 0.079 g Co(NO3)2·6H2O 49.4 mg Distilled water 1.0 L Christensen citrate agar An organic-rich, solid microbiological culture medium containing L-cysteine, D-glucose, citrate, yeast extract, a pH buffer and a pH indicator. https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/3/c7595dat.pdf Product Description Christensen Citrate Agar is used for the differentiation of enteric pathogens and coliforms on the basis of citrate utilization. Organisms that metabolize citrate as a sole source of carbon cleave citrate to oxaloacetate and acetate via the citritase enzyme. Another enzyme, oxaloacetate decarboxylase, then converts oxaloacetate to pyruvate and CO2 . The CO2 combines with sodium and water to form sodium carbonate, an alkaline compound. As a result, the pH of the medium rises and the phenol red indicator changes from orange red to cerise. The yeast extract provides the necessary vitamins for the growth of the microorganisms. L- cysteine hydrochloride functions as a reducing agent. Dextrose is the fermentable carbohydrate. Sodium citrate is the energy source for the citrate utilizing organisms. Care should be taken while inoculating, as a too heavy inoculum may give a false positive result (4). Components Item g/L Yeast Extract 0.50 L-Cysteine Hydrochloride 0.10 Sodium Citrate 3.00 Dextrose 0.20 Monopotassium Phosphate 1.00 Sodium Chloride 5.00 Phenol Red 0.012 Agar 15.00 Final pH (at 25°C) 6.9 ± 0.2 Carrine Blank cetrimide nalidixic agar https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Fluka/Datasheet/11012dat.pdf 11012 Cetrimide Nalidixic acid Agar For the isolation and enumeration (MPN method) of Pseudomonas aeruginosa from food products. Recommended by the "Schweizerisches Lebensmittelbuch" 2001., chapter 56A. Composition: Gelatine peptone, pancreatic 16.0 Casein hydrolysate 10.0 Potassium sulfate 10.0 Magnesium chloride 1.4 Cetrimide 0.2 Agar 15.0 Final pH 7.3+/- 0.2 at 37 °C Directions: Dissolve 52.6 g in 950 ml distilled water, add 10 ml glycerol (Fluka 49769) and sterilize by autoclaving at 121°C for 15 minutes. After cooling to 45-50°C add the rehydrate content of 1 vial Cetrimide Nalidixic acid Agar Supplement (Fluka 50225). Mix well. The prepared agar may contain slight precipitates. Principle and Interpretation: Gelatine peptone and Casein hydrolysate provides amino acids and other complex nitrogenous substances. Cetrimide (Cetyltrimethylammonium bromide; Fluka 52370) is incorporated in the medium to inhibit bacteria other than Pseudomonas aeruginosa. lt acts as a quaternary ammonium compound and cationic detergent which causes nitrogen and phosphorus to be released from bacterial cells other than Pseudomonas aeruginosa. Nalidixic acid present in the supplement improve the inhibition of the accompanying microbial flora (3, 4). For the isolation of Pseudomonas aeruginosa, plates of Cetrimide Agar should be inoculated from non-selective medium such as Brain Heart Infusion Broth (Fluka 70138) or Tryptone Soya Broth (Fluka 22092). If the count is high the test sample can be directly inoculated onto this medium. According the Schweizerisches Lebensmittelbuch (2) it is recommended to to put membrane filter, with different dilutions, directly on the agar plates. Glycerol is the carbon source. Magnesium chloride and potassium sulfate enhance the production pycocyanin and fluorescein. Pseudomonas aeruginosa colonies appear pigmented blue, bluegreen, red or nonpigmented but are fluorescent under UV light (360nm). An organic-rich, solid microbiological culture medium that contains cetrimide and nalidixic acid. Carrine Blank nalidixic acid-cetrimide agar coagulated egg medium http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=6&ved=0CEEQFjAF&url=http%3A%2F%2Fwww.bd.com%2Feurope%2Fregulatory%2FAssets%2FIFU%2FHB%2FCE%2FBA%2FBA-257165.pdf&ei=L0_ZVJ6KCs-ryATu2YL4DQ&usg=AFQjCNH2fsKGxD4irxeJNurPKjLHCctpjA&sig2=T-2Ahw_G8MVq6GlpMrCnwQ&bvm=bv.85464276,d.aWw INTENDED USE BD Dorset Egg Medium, Modified is a prepared tubed, slanted, coagulated egg medium used for cultivation, maintenance and transport of pure cultures of mycobacteria and other fastidious and nonfastidious organisms. PRINCIPLES AND EXPLANATION OF THE PROCEDURE Microbiological method. BD Dorset Egg Medium, Modified is a modification of the whole egg medium described by Dorset.1 It is a nonselective medium well suited for the growth and maintenance of pure cultures of mycobacteria.2 More recently, it has also been used for the maintenance and transport of other bacterial species, such as Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influenzae, and enterotoxigenic E. coli at ambient temperature.3-5 Beef extract and peptone provide nutrients such as amino acids and organic phosphates. Whole egg mass contains complex nutrients necessary for bacterial and mycobacterial growth and, additionally, neutralizes toxic compounds. The inspissation process during the preparation of the medium provides the necessary solidity of the medium and inactivates bactericidal compounds contained in eggs such as lysozyme. REAGENTS BD Dorset Egg Medium, Modified Formula* Per Liter Purified Water Beef Extract 3.0 Peptone 5.0 Egg Mass (from whole fresh eggs) 750 ml pH 7.2 +/- 0.3 An organic-rich, liquid microbiological culture medium containing peptone, beef extract, and coagulated egg. Used to grow Mycobacteria. Carrine Blank coagulated egg http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=6&ved=0CEEQFjAF&url=http%3A%2F%2Fwww.bd.com%2Feurope%2Fregulatory%2FAssets%2FIFU%2FHB%2FCE%2FBA%2FBA-257165.pdf&ei=L0_ZVJ6KCs-ryATu2YL4DQ&usg=AFQjCNH2fsKGxD4irxeJNurPKjLHCctpjA&sig2=T-2Ahw_G8MVq6GlpMrCnwQ&bvm=bv.85464276,d.aWw INTENDED USE BD Dorset Egg Medium, Modified is a prepared tubed, slanted, coagulated egg medium used for cultivation, maintenance and transport of pure cultures of mycobacteria and other fastidious and nonfastidious organisms. PRINCIPLES AND EXPLANATION OF THE PROCEDURE Microbiological method. BD Dorset Egg Medium, Modified is a modification of the whole egg medium described by Dorset.1 It is a nonselective medium well suited for the growth and maintenance of pure cultures of mycobacteria.2 More recently, it has also been used for the maintenance and transport of other bacterial species, such as Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influenzae, and enterotoxigenic E. coli at ambient temperature.3-5 Beef extract and peptone provide nutrients such as amino acids and organic phosphates. Whole egg mass contains complex nutrients necessary for bacterial and mycobacterial growth and, additionally, neutralizes toxic compounds. The inspissation process during the preparation of the medium provides the necessary solidity of the medium and inactivates bactericidal compounds contained in eggs such as lysozyme. REAGENTS BD Dorset Egg Medium, Modified Formula* Per Liter Purified Water Beef Extract 3.0 Peptone 5.0 Egg Mass (from whole fresh eggs) 750 ml pH 7.2 +/- 0.3 Carrine Blank The homogenized whole egg of a chicken that has been coagulated by the process of inspissation (drying), used in the cultivation of microorganisms. Murashige and Skoog agar MS medium A minerals-salts, solid culture medium containing ammonium nitrate, calcium chloride, magnesium sulfate, potassium phosphate, and potassium nitrate as major salts. Used for the cultivation of plant cells (for plant cell culture). MS0 http://en.wikipedia.org/wiki/Murashige_and_Skoog_medium Murashige and Skoog medium or (MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture. MSO was invented by plant scientists Toshio Murashige and Folke K. Skoog in 1962 during Murashige's search for a new plant growth regulator. A number behind the letters MS is used to indicate the sucrose concentration of the medium. For example MS0 contains no sucrose and MS20 contains 20 g/l sucrose. Along with its modifications, it is the most commonly used medium in plant tissue culture experiments in laboratorium.[1] Ingredients Major salts (macronutrients) Ammonium nitrate (NH4NO3) 1,650 mg/l Calcium chloride (CaCl2 · 2H2O) 440 mg/l Magnesium sulphate (MgSO4 · 7H2O) 370 mg/l Potassium phosphate (KH2PO4) 170 mg/l Potassium nitrate (KNO3) 1,900 mg/l Minor salts (micronutrients) Boric acid (H3BO3) 6.2 mg/l Cobalt chloride (CoCl2 · 6H2O) 0.025 mg/l Cupric sulphate (CuSO4 · 5H2O) 0.025 mg/l Ferrous sulphate (FeSO4 · 7H2O) 27.8 mg/l Manganese sulphate (MnSO4 · 4H2O) 22.3 mg/l Potassium iodide (KI) 0.83 mg/l Sodium molybdate (Na2MoO4 · 2H2O) 0.25 mg/l Zinc sulphate (ZnSO4·7H2O) 8.6 mg/l Na2EDTA · 2H2O 37.2 mg/l Vitamins and organics i-Inositol 100 mg/l Niacin 0.5 mg/l Pyridoxine · HCl 0.5 mg/l Thiamine · HCl 0.1 mg/l IAA 1–30 mg/l Kinetin 0.04–10 mg/l Glycine (recrystallized) 2.0 mg/l Edamin (lactalbumin hydrolysate) 1.0 g/l Agar 10 g/l An optimum pH of 5.8 should be maintained. Carrine Blank MSO Gifu anaerobic medium Carrine Blank An organic-rich, liquid microbiological culture medium containing L-cysteine, D-glucose, starch, the extracts of liver, beef and yeast, peptones, digested serum, and sodium thioglycollate. Thioglycollate and L-cysteine are reducing agents to provide anaerobiosis. GAM broth http://www.himedialabs.com/TD/M1801.pdf Ingredients Gms / Litre Peptic digest of animal tissue 10.000 Papaic digest of soyabean meal 3.000 Proteose peptone 10.000 Digested serum 13.500 Yeast extract 5.000 Beef extract 2.200 Liver extract 1.200 Dextrose 3.000 Potassium dihydrogen phosphate 2.500 Sodium chloride 3.000 Starch, Soluble 5.000 L-Cysteine hydrochloride 0.300 Sodium thioglycollate 0.300 Final pH ( at 25°C) 7.3±0.1 Principle And Interpretation Gifu Anaerobic Medium (GAM Broth) is a liquid medium for anaerobic bacteria. As this medium contains the digested serum which has hemin, it is successfully used for cultivation of anaerobic organisms such as streptococci, pneumonococci and meningococci. This medium is also suitable for blood culture (1). Anaerobic organisms require reducing condition and an absence of dissolved oxygen in the medium. Strict anaerobes obtain its energy and intermediates through oxidation utilizing hydrogen acceptors other than oxygen. Pre-reducing the medium by boiling to drive off the oxygen can expel this (2). Sodium thioglycollate and L-Cysteine are the reducing agents added in this medium to provide adequate anaerobiosis. Anaerobic bacteria vary in their sensitivy to oxygen and nutritional requirements (3). Peptic digest of animal tissue and yeast extract provide nitrogen, carbon and vitamin source. Starch absorbs the toxic metabolites produced (4). Hemin serves as essential growth factor and Sodium chloride maintains osmotic equilibrium (5). Moeller KCN broth KCN broth Carrine Blank From: Moeller KCN Broth Base (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Moeller KCN Broth Base, when supplemented with a solution of potassium cyanide, is used in the differentiation of enteric bacilli on the basis of their ability to grow promptly in the presence of cyanide. Principles of the Procedure The addition of 0.15 mL of a 0.5% solution of potassium cyanide to each of the prepared tubes of the nutritive base enables differentiation of members of various genera within the Enterobacteriaceae family. Moeller KCN Broth Base Approximate Formula* Per Liter Purified Water Pancreatic Digest of Casein ........................................1.5 g Peptic Digest of Animal Tissue ..................................1.5 g Sodium Chloride ..........................................................5.0 g Monopotassium Phosphate ........................................0.225 g Disodium Phosphate ....................................................5.64 g pH 7.6 DSMZ Medium 1071 DSM strains: An organic-rich, liquid culture medium comprised of polypeptone, yeast extract, sodium chloride, magnesium chloride, calcium chloride, sodium molybdate, copper chloride, and ferric chloride. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1071.pdf 1071. PY-BROTH Polypepton 2.000 g Bacto yeast extract (Difco) 0.500 g NaCl 30.000 g MgCl2 x 6 H2O 5.000 g CaCl2 x 2 H2O 0.005 g Na2MoO4 x 7 H2O 0.005 g CuCl2 x 2 H2O 0.004 g FeCl3 x 6 H2O 0.006 g Agar 15.000 g Distilled water 1000.000 ml pH 8.0 © 2007 DSMZ GmbH - All rights reserved Carrine Blank peptone yeast broth PY medium PY broth Eugon agar From: Eugon Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Eugon Agar is a general-purpose medium used for cultivating a wide variety of microorganisms. Principles of the Procedure Peptones provide the nitrogen, vitamins and amino acids in Eugon Agar. The high concentration of dextrose is the energy source for rapid growth of bacteria. L-Cystine and sodium sulfite are added to stimulate growth. Sodium chloride maintains the osmotic balance of the media. The high carbohydrate content along with high sulfur (cystine) content improves growth with chromogenicity.2 Agar is the solidifying agent in Eugon Agar. Formula Difco™ Eugon Agar Approximate Formula* Per Liter Proteose Peptone No. 3................................................ 7.5 g Pancreatic Digest of Casein.......................................... 7.5 g Soy Peptone................................................................. 5.0 g Dextrose...................................................................... 5.5 g L-Cystine...................................................................... 0.7 g Sodium Chloride.......................................................... 4.0 g Sodium Sulfite.............................................................. 0.2 g Agar.......................................................................... 15.0 g pH 7.0 An organic-rich, solid microbiological culture medium containing L-cystine, D-glucose, peptones, and sodium sulfite. Carrine Blank barophile 1.0 1.0 Prokaryote specialized with respect to pressure, which grows optimally at elevated pressures. Carrine Blank Eugon broth Eugonbroth An organic-rich, liquid microbiological culture medium containing L-cystine, D-glucose, peptones, and sodium sulfite. Carrine Blank From: Eugon Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Eugon Broth (Eugonbroth™) is a general-purpose medium used for the cultivation of fastidious and nonfastidious bacteria from a variety of clinical and nonclinical specimens. Principles of the Procedure Peptones supply amino acids and other nitrogenous substances to support bacterial growth. L-cystine is an essential amino acid that improves growth. Dextrose is incorporated as a source of energy and sodium chloride provides osmotic equilibrium. Sodium sulfite along with the cystine content improves growth with chromogenicity. Formula Bacto™ Eugon Broth Approximate Formula* Per Liter Proteose Peptone No. 3................................................ 7.5 g Pancreatic Digest of Casein.......................................... 7.5 g Soy Peptone................................................................. 5.0 g Dextrose...................................................................... 5.5 g L-Cystine...................................................................... 0.7 g Sodium Chloride.......................................................... 4.0 g Sodium Sulfite.............................................................. 0.2 g pH 7.0 obligate barophile 1.0 1.0 Carrine Blank A barophile, which grows only at elevated pressures. digested serum Carrine Blank Microbiological medium ingredient, derived from the acid hydrolysis of proteins in (blood) serum. liver extract An aqueous (water) extract of the dried liver of a mammal (typically a cow), used in the cultivation of microorganisms. Carrine Blank liver infusion http://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=13 To prepare liver extract, put 10 g liver powder in 170 ml water, keep at 50 to 60C for 1 hr, boil for 5 min, adjust pH to 7.2 and filter. facultative barophile 1.0 1.0 Carrine Blank Prokaryote specialized with respect to pressure, which grows at elevated pressures and at atmospheric pressure. lactalbumin hydrolysate edamin An enzymatic hydrolysate of lactalbumin (a milk protein from cow's milk, Bos taurus) made using hydrolytic enzymes, used for the culturing of microorganisms. Carrine Blank http://www.neogen.com/Acumedia/pdf/ProdInfo/7465_PI.pdf Intended Use Lactalbumin Hydrolysate is an enzymatic digest of lactalbumin for use in preparing microbiological culture media. Principles of the Procedure Lactalbumin Hydrolysate provides nitrogen, amino acids, vitamins, and carbon in microbiological culture media. casein hydrolysate Microbiological medium ingredient, derived from enzymatic hydrolysis of milk protein. An enzymatic hydrolysate of casein (a milk protein from cow's milk, Bos taurus), used for the culturing of microorganisms. Carrine Blank halophile 1.7 1.7 Carrine Blank Prokaryote specialized with respect to salinity, which grows optimally at salinities of > 1.7%. Gs medium From: Hai-Qin Tan, Tian-Tian Li, Chu Zhu, Xin-Qi Zhang, Min Wu and Xu-Fen Zhu. 2012. Parabacteroides chartae sp. nov., an obligately anaerobic species from wastewater of a paper mill. IJSEM 62:2613-2617. The initial enrichment used medium Gs [containing l-1 distilled water: 10 g NaCl, 1.0 g MgCl2 . 6H2O, 0.5 g K2HPO4, 0.7 g KH2PO4, 0.025 g FeSO4 . 7H2O, 0.2 g CaCl2 . 2H2O, 1.0 g urea, 5 g yeast extract (Difco), 5 g tryptone, 1 ml trace element solution SL-10 (Widdel et al., 1983), 0.4 g L-cysteine and 0.001 g resazurin based on phosphate buffer pH table, the pH of this medium should be around 6.7. http://www.uslims.uthscsa.edu/po4buffers.php Carrine Blank An organic-rich, liquid microbiological culture medium containing urea, L-cysteine, resazurin, tryptone, and yeast extract. Used for the cultivation of Parabacteroides chartae. trace elements solution SL-10 A trace elements solution containing hydrochloric acid, ferrous chloride, cobalt chloride, manganese chloride, zinc chloride, boric acid, sodium molybdate, nickel chloride, and copper chloride. Carrine Blank From: ATCC Medium 2734 Trace element solution SL10 The components of the trace element solutions SL10 (Widdel et al. 1983) are added and dissolved in the order listed. 25% HCl……………………………………………….10 ml FeCl2x4H2O………………………………………….1.5 g CoCl2x6H2O………………………………………….190 mg MnCl2x4H2O………………………………………….100 mg ZnCl2…………………………………………………..70 mg H3BO3………………………………………………….6 mg Na2MoO4x2H2O……………………………………..36 mg NiCl2x6H2O…………………………………………..24 mg CuCl2x2H2O………………………………………….2 mg DI Water……………………………………………….1000 ml The trace element solution is autoclaved under air, in 25 ml aliquots in 50 ml screw-capped bottles. Large stocks do not need to be sterilized for storage. microbiological medium ingredient, derived from aqueous extracts Carrine Blank Undefined mixture of complex organic compounds derived from animal, fish, plant materials or soil, produced by the process of aqueous extraction (extraction using water, such as hot water). Used in the cultivation of microorganisms. Gram-positive bacteria Carrine Blank Gram-negative bacteria Carrine Blank obligate halophile Carrine Blank Halophilies, which only growt at salinities of > 1.7%. facultative halophile Prokaryote specialized with respect to salinity, which can grow at salinities of > 1.7% or at lower salinities. Carrine Blank facultative thermophile Prokaryote specialized with respect to temperature, which grows optimally at moderate temperatures (20-45˚C), but can tolerate higher temperatures. Carrine Blank DSMZ Medium 1071 agar DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1071.pdf 1071. PY-BROTH Polypepton 2.000 g Bacto yeast extract (Difco) 0.500 g NaCl 30.000 g MgCl2 x 6 H2O 5.000 g CaCl2 x 2 H2O 0.005 g Na2MoO4 x 7 H2O 0.005 g CuCl2 x 2 H2O 0.004 g FeCl3 x 6 H2O 0.006 g Agar 15.000 g Distilled water 1000.000 ml pH 8.0 © 2007 DSMZ GmbH - All rights reserved PY agar An organic-rich, solid culture medium comprised of polypeptone, yeast extract, sodium chloride, magnesium chloride, calcium chloride, sodium molybdate, copper chloride, ferric chloride and agar. Carrine Blank mildly reducing microbiological culture medium A microbiological culture medium containing mild reducing agents (sodium thioglycollate or L-cysteine) which maintain a moderately low redox and hence promote the growth of facultative anaerobes. Carrine Blank strongly reducing microbiological culture medium A microbiological culture medium containing strong reducing agents (such as sodium sulfide) which maintain a very low redox and hence promote the growth of anaerobes and strict anaerobes. Carrine Blank pH quality Culture medium quality with respect to the concentration of hydrogen ions, on a logarithmic scale. Carrine Blank strongly acidic pH Carrine Blank Culture medium where the pH value is below 4.0. acidic pH Carrine Blank Culture medium where the pH value is below 6.5. near neutral pH Carrine Blank Culture medium where the pH value is between 6.5 and 7.5. alkaline pH Carrine Blank Culture medium where the pH value is above 7.5. strongly alkaline pH Culture medium where the pH value is above 10.0. Carrine Blank strongly reducing redox Strongly reducing conditions dominated by the presence of cysteine, glutathione, 2-mercaptoethanol, dithiothreitol, sodium sulfide (hydrogen sulfide), dithionite, or titanium citrate. Carrine Blank mildly reducing redox Weakly reducing conditions dominated by the presence of organosulfides or thiosulfate as the reducing agent, but not sodium sulfide or hydrogen sulfide. zzzz Carrine Blank oxidizing redox Carrine Blank Oxidizing conditions due to the presence of oxygen. hyperacidophile 4.0 Carrine Blank Prokaryote specialized with respect to pH, which grows optimally at extremely acidic pH values (pH <4). acidophile 4.0 6.0 Prokaryote specialized with respect to pH, which grows optimally at moderately acidic pH values (pH 4-6). Carrine Blank hyperalkaliphile 10.0 Prokaryote specialized with respect to pH, which grows optimally at extremely alkaline pH values (pH >10). Carrine Blank obligate acidophile 4.0 6.0 Alcidophile, only grows between pH 4-6. Carrine Blank alkaliphile 8.0 10.0 Carrine Blank Prokaryote specialized with respect to pH, which grows optimally at moderately alkaline pH values (pH 8-10). prokaryote specialized with respect to pH Carrine Blank Prokaryotic physiologically differentiated cell, where the cell is specialized with resect to its optimal growth pH. oxidizing microbiological culture medium A microbiological culture medium lacking reducing agents (such as thioglycollate, L-cysteine, or sodium sulfide) while also having oxygen present. Promotes the growth of facultative anaerobes and aerobes. Carrine Blank redox quality Culture medium quality with respect to the oxidation-reduction potential of the medium. Carrine Blank obligate hyperacidophile 4.0 Hyperalcidophile, only grows below pH 4. Carrine Blank neutrophile 6.0 8.0 Carrine Blank Prokaryote specialized with respect to pH, which grows optimally at circumneutral pH values (pH 6-8). obligate alkaliphile 8.0 10.0 Carrine Blank Alkaliphile, only grows between pH 8-10. obligate hyperalkaliphile 10.0 Carrine Blank Hyperalkaliphile, only grows above pH 10. moderately alkaline pH Carrine Blank Culture medium where the pH value is between 8.5 and 10. slightly alkaline pH Culture medium where the pH value is between 7.5 and 8.5. Carrine Blank slightly acidic pH Carrine Blank Culture medium where the pH value is between 5.5 and 6.5. moderately acidic pH Carrine Blank Culture medium where the pH value between 5.5 and 4.0. strongly acidic microbiological culture medium Carrine Blank Microbiological culture medium with a pH value below 4.0. moderately acidic microbiological culture medium Microbiological culture medium that has a pH value between 5.5 and 4.0. Carrine Blank slightly acidic microbiological culture medium Carrine Blank Microbiological culture medium with a pH value between 5.5 and 6.5. near neutral pH microbiological culture medium Microbiological culture medium that has a pH value between 6.5 and 7.5. Carrine Blank slightly alkaline microbiological culture medium Microbiological culture medium with a pH value between 7.5 and 8.5. Carrine Blank moderately alkaline microbiological culture medium Microbiological culture medium with a pH value between 8.5 and 10. Carrine Blank strongly alkaline microbiological culture medium Carrine Blank Microbiological culture medium with a pH value above 10.0. alkaline microbiological culture medium Carrine Blank Microbiological culture medium that has a pH value less than 6.5. acidic microbiological culture medium Microbiological culture medium that has a pH value above 8.5. Carrine Blank aerotolerant prokaryote A prokaryote which is capable of growth in the absence of oxygen. Can grow or live in the presence of oxygen. Carrine Blank facultative psychrophile Carrine Blank Prokaryote specialized with respect to temperature, which grows optimally at moderate temperatures (20-45˚C), but can tolerate lower temperatures. microbiological assay of lysis of blood cells Microbiological reactivity to lymphatic materials assay, where the assay is for the cytolysis (lysis) of blood cells. Carrine Blank microbiological assay of inhibition of blood clotting Carrine Blank A microbiological diagnostic assay that tests for the inhibition of blood clotting using blood plasma. microbiological assay of promotion of blood clotting A microbiological ractivity to lymphatic materials assay, which assays for the promotion of blood clotting. Carrine Blank microbiological assay of binding to blood cells Carrine Blank A microbiological reactivity to blood assay, to determine whether a microorganism causes blood cells to clump or a microorganism can adhere to blood cells. hemolysis A process of cytolysis, where a microorganism secretes a protein(s) which results in the formation of a pore in the membranes of erythrocytes (red blood cells) or leucocytes (white blood cells) and thus causing the cells to lyse. Carrine Blank Wikipedia:hemolysis_(microbiology) An assay for the capability of a microorganism to lyse red blood cells (hemolysis, haemolysis). Hemolysis is induced by the secretion of various hemolysins by particular pathogenic strains of bacteria. Hemolysins can be proteins or lipids which act by disrupting the membranes of red blood cells. alpha-hemolysis Wikipedia:hemolysis_(microbiology) Alpha-haemolysis occurs when an organism secretes alpha-haemolysin, a protein which forms a pore in the membranes of red blood cells. This leads to the formation of colonies on blood agar that have a dark greenish appearance. The green color is caused by peroxidation of hemoglobin (presence of biliverdin). Carrine Blank A process of hemolysis, where a protein secreted by a microorganism results in the formation of a pore in the membranes of erythrocytes (red blood cells) and thus causing the cells to lyse. beta-hemolysis Wikipedia:hemolysis_(microbiology) Beta hemolysis (β-hemolysis), is caused by the secretion of beta-hemolysin by a pathogenic bacterium. Beta-hemolysin is a protein which catalyzes the hydrolysis of sphingomyelin, releasing phosphoryl choline and resulting in lysis fo the red blood cell. The result are colonies on blood agar that are surrounded by a yellow, transparent zone. A process of hemolysis, where a protein (beta-hemolysin) secreted by a microorganism results in the hydrolysis of sphingomyelin in erythrocytes (red blood cells) and thus causing the cells to lyse. Carrine Blank gamma-hemolysis Carrine Blank Wikipedia:hemolysis_(microbiology) Gamma hemolysis, caused by the secretion of gamma-hemolysin, results in the lysis of leucocytes (white blood cells). Red blood cells are unaffected, and therefore do not show hemolytic activity on blood agar plates. A process of hemolysis, where a protein (gamma-hemolysin) secreted by a microorganism results in the formation of a pore in the membranes of leukocytes (white blood cells) and thus causing the cells to lyse. gamma-hemolysin Wikipedia: hemolysin γ-Hemolysin Unlike beta-hemolysin, it has a higher affinity for phosphocholines with short saturated acyl chains, especially if they have a conical form, whereas cylindrical lipids (e.g., sphingomyelin) hinder its activity. The lytic process, most commonly seen in leucocytes, is caused by pore formation induced by an oligomerized octamer that organizes in a ring structure. Once the prepore is formed, a more stable one ensues, named β-barrel. In this final part, the octamer binds with phosphatidylcholine.[8] Carrine Blank A hemolysin protein secreted by Staphylococcus aureus. Form pores in target blood cells. gamma-haemolysin From: Dalla Serra M, Coraiola M, Viero G, Comai M, Potrich C, Ferreras M, Baba-Moussa L, Colin DA, Menestrina G, Bhakdi S, Prévost G. 2005. Staphylococcus aureus bicomponent gamma-hemolysins, HlgA, HlgB, and HlgC, can form mixed pores containing all components. J Chem Inf Model 45(6):1539-45. Staphylococcal gamma-hemolysins are bicomponent toxins forming a protein family with leucocidins and alpha-toxin. Two active toxins (AB and CB) can be formed combining one of the class-S components, HlgA or HlgC, with the class-F component HlgB. These two gamma-hemolysins form pores with marked similarities to alpha-toxin in terms of conductance, nonlinearity of the current-voltage curve, and channel stability in the open state. AB and CB pores, however, are cation-selective, whereas alpha-toxin is anion-selective. gamma-Hemolysins' pores are hetero-oligomers formed by three or four copies of each component (indicated as 3A3B and 3C3B or 4A4B and 4C4B). Point mutants located on a beta-strand of the class-S component that forms part of the protomer-protomer contact region can prevent oligomer assembly. Interestingly, these mutants inhibit growth of pores formed not only by their natural components but also by nonstandard components. This lead to the hypothesis that mixed ABC pores could also be formed. By studying the conductance of pores, assembled in the presence of all three components (in different ratios), it was observed that the magnitudes expected for mixed pores were, indeed, present. We conclude that the gamma-hemolysin/leucocidin bicomponent toxin family may form a larger than expected number of active toxins by cross-combining various S and F components. beta-hemolysin beta-haemolysin A hemolysin protein secreted by Group A Streptococci (Streptolysin O and Streptolysin S) which causes complete lysis of red blood cells, polymorphonuclear leukocytes (granulocytes), and lymphocytes. Also, a beta-hemolysin protein is secreted by Staphylococcus aureus which involves the hydrolysis of sphingomyelin, which causes cell death. Carrine Blank Wikipedia: Hemolysin β-hemolysin Upon investigating sheep erythrocytes, its toxic mechanism was discovered to be the hydrolysis of a specific membrane lipid, sphingomyelin, which accounts for 50% of the cell’s membrane. This degradation was followed by a noticeable rise of phosphoryl-choline due to the release of organic phosphorus from sphingomyelin and ultimately caused cell lysis.[7] alpha-hemolysin Hla A hemolysin protein secreted by Staphylococcus aureus, which forms a pore in the membranes of human immune cells, in particular T-cells, monocytes, and peripheral blood lymphocytes. Pore formation results in apoptosis of the immune cell, resulting in cell lysis and death. Wikipedia:Staphylococcus aureus alpha toxin Alpha-toxin, also known as alpha-hemolysin (Hla), is the major cytotoxic agent released by bacterium Staphylococcus aureus and the first identified member of the pore forming beta-barrel toxin family.[1] This toxin consists mostly of beta-sheets (68%) with only about 10% alpha-helices. The hla gene on the S. aureus chromosome encodes the 293 residue protein monomer, which forms heptameric units on the cellular membrane to form a complete beta-barrel pore. This structure allows the toxin to perform its major function, development of pores in the cellular membrane, eventually causing cell death. alpha-toxin alpha-haemolysin Carrine Blank hemolysin Wikipedia: Hemolysin Hemolysins (UK spelling: haemolysins) are lipids and proteins that cause lysis of red blood cells by destroying their cell membrane. Although the lytic activity of some microbe-derived hemolysins on red blood cells may be of great importance for nutrient acquisition, many hemolysins produced by pathogens do not cause significant destruction of red blood cells during infection. Although hemolysins are capable of doing this for red blood cells in vitro. As mentioned above, most hemolysins are protein compounds, but others are lipids biosurfactants.[1] Carrine Blank haemolysin Lipids or proteins that cause the lysis (cytolysis) of blood cells or immune cells. tryptic soy agar with sheep blood Carrine Blank An organic-rich, solid medium containing animal and plant peptones and sodium chloride. Supplemented with sheep blood. Streptolysin O Carrine Blank A beta protein found in Streptococcus, acts with Streptolysin S to form cell pores which cause hemolysis. Streptolysin S Carrine Blank A beta protein found in Streptococcus, acts with Streptolysin O to form cell pores which cause hemolysis. mesophile 20.0 45.0 20.0 45.0 Carrine Blank Prokaryote specialized with respect to temperature, which grows optimally at moderate temperatures (20-45˚C). HlgA protein Carrine Blank A gamma-hemolysin protein found in Staphylococcus aureus, acts with HlgB and HlgC to form cell pores which cause hemolysis. HlgB protein A gamma-hemolysin protein found in Staphylococcus aureus, acts with HlgA and HlgC to form cell pores which cause hemolysis. Carrine Blank HlgC protein Carrine Blank A gamma-hemolysin protein found in Staphylococcus aureus, acts with HlgA and HlgB to form cell pores which cause hemolysis. sunlight Electromagnetic radiation from the Sun that reaches the surface of the Earth. Wikipedia: Light Light is electromagnetic radiation within a certain portion of the electromagnetic spectrum. The word usually refers to visible light, which is visible to the human eye and is responsible for the sense of sight.[1] Visible light is usually defined as having a wavelength in the range of 400 nanometres (nm), or 400×10−9 m, to 700 nanometres – between the infrared (with longer wavelengths) and the ultraviolet (with shorter wavelengths).[2][3] Often, infrared and ultraviolet are also called light. The main source of light on Earth is the Sun. Sunlight provides the energy that green plants use to create sugars mostly in the form of starches, which release energy into the living things that digest them. This process of photosynthesis provides virtually all the energy used by living things. Historically, another important source of light for humans has been fire, from ancient campfires to modern kerosene lamps. With the invention of electricity, electric lighting has all but replaced firelight. Some species of animals generate their own light, called bioluminescence. For example, fireflies use light to locate mates, and vampire squids use it to hide themselves from prey. Carrine Blank heterotrophy A prokaryotic metabolic process where the carbon or energy sources are organic compounds. From: Worden AZ, Follows MJ, Giovannoni SJ, Wilken S, Zimmerman AE, Keeling PJ. 2015. Rethinking the marine carbon cycle: Factoring in the multifarious lifestyles of microbes. Science 347, DOI: 10.1126/science.1257594. (ii) Osmotrophy: Cells take up organic material from the external environment as small molecules or macromolecules. osmotrophy Carrine Blank autotrophy From: Worden AZ, Follows MJ, Giovannoni SJ, Wilken S, Zimmerman AE, Keeling PJ. 2015. Rethinking the marine carbon cycle: Factoring in the multifarious lifestyles of microbes. Science 347, DOI: 10.1126/science.1257594. (i) Primary producer: Generates organic carbon by photosynthesis and CO2 fixation (the role traditionally played by phytoplankton). A prokaryotic respiratory metabolic process where carbon dioxide is the source of carbon. Carrine Blank primary production prokaryotic metabolically differentiated cell Carrine Blank A prokaryotic differentiated cell that carries out a specific metabolic role, and is therefore metabolically differentiated. nano-sized cell 2.0 20.0 2.0 20.0 Carrine Blank nano-sized A prokaryotic cell, where the width of the cell is between 2 and 20 microns (um). autotroph A prokaryote capable of carrying out the role of autotrophy, where carbon dioxide is the source of carbon. Carrine Blank Wikipedia: Autotroph An autotroph[α] ("self-feeding", from the Greek autos "self" and trophe "nourishing") or "producer", is an organism that produces complex organic compounds (such as carbohydrates, fats, and proteins) from simple substances present in its surroundings, generally using energy from light (photosynthesis) or inorganic chemical reactions (chemosynthesis). They are the producers in a food chain, such as plants on land or algae in water, in contrast to heterotrophs as consumers of autotrophs. They do not need a living energy or organic carbon source. Autotrophs can reduce carbon dioxide to make organic compounds for biosynthesis and also create a store of chemical energy. Most autotrophs use water as the reducing agent, but some can use other hydrogen compounds such as hydrogen sulfide. Phototrophs (green plants and algae), a type of autotroph, convert physical energy from sunlight into chemical energy in the form of reduced carbon. pico-sized cell 0.2 2.0 0.2 2.0 pico-sized Carrine Blank A prokaryotic cell, where the width of the cell is between 0.2 and 2 microns (um). obligate mesophile 20.0 45.0 20.0 45.0 A mesophile which can only grow at moderate temperatures (20-40˚C). Carrine Blank psychrophile 20.0 20.0 Carrine Blank Prokaryote specialized with respect to temperature, which grows optimally at depressed temperatures (< 20˚C). heterotroph From: Worden AZ, Follows MJ, Giovannoni SJ, Wilken S, Zimmerman AE, Keeling PJ. 2015. Rethinking the marine carbon cycle: Factoring in the multifarious lifestyles of microbes. Science 347, DOI: 10.1126/science.1257594. (viii) Symbioses: Defined here as mutualistic relationships where one species lives on or within another species (Fig. 3 inset shows a diatom with N2-fixing cyanobacteria on its spines). Wikipedia: Heterotroph A heterotroph (/ˈhɛtərɵtroʊf/; ἕτερος heteros = "another", "different" and τροφή trophe = "nutrition") is an organism that cannot fix carbon and uses organic carbon for growth.[1][2] Heterotrophs can be further divided based on how they obtain energy; if the heterotroph uses light for energy, then it is considered a photoheterotroph, while if the heterotroph uses chemical energy, it is considered a chemoheterotroph. Carrine Blank A prokaryote capable of carrying out the role of heterotrophy where the carbon or energy sources are organic compounds. planktonic environment euplanktonic An environmental system occupied by a microorganism that floats in a column of water, where the organisms cannot actively swim against the current. planktic planktonic cells free floating Wikipedia: Plankton Plankton (singular plankter) are a diverse group of organisms that live in the water column and cannot swim against a current.[1] They provide a crucial source of food to many large aquatic organisms, such as fish and whales. These organisms include drifting animals, protists, archaea, algae, or bacteria that inhabit the pelagic zone of oceans, seas, or bodies of fresh water; that is, plankton are defined by their ecological niche rather than phylogenetic or taxonomic classification. Though many planktonic species are microscopic in size, plankton includes organisms covering a wide range of sizes, including large organisms such as jellyfish.[2] The adjective planktonic is widely used in both the scientific and popular literature, and is a generally accepted term. However, from the standpoint of formal grammar the less commonly used planktic is more strictly the correct adjective. When deriving English words from their Greek or Latin roots the gender specific ending (in this case "-on," which indicates the word is neuter) is normally dropped, using only the root of the word in the derivation.[7] Carrine Blank phototroph A prokaryote capable of carrying out the role of phototrophy, where light is the energy source. Carrine Blank lithotrophy A prokaryotic respiratory metabolic process where the oxidation and reduction of inorganic chemical compounds is the energy source. Wikipedia: Lithotroph Lithotrophs are a diverse group of organisms using inorganic substrate (usually of mineral origin) to obtain reducing equivalents for use in biosynthesis (e.g., carbon dioxide fixation) or energy conservation (i.e., ATP production) via aerobic or anaerobic respiration.[1] Known chemolithotrophs are exclusively microbes; no known macrofauna possesses the ability to utilize inorganic compounds as energy sources. Macrofauna and lithotrophs can form symbiotic relationships, in which case the lithotrophs are called "prokaryotic symbionts". An example of this is chemolithotrophic bacteria in giant tube worms or plastids, which are organelles within plant cells that may have evolved from photolithotrophic cyanobacteria-like organisms. Lithotrophs belong to either the domain Bacteria or the domain Archaea. The term "lithotroph" was created from the Greek terms 'lithos' (rock) and 'troph' (consumer), meaning "eaters of rock". Many lithoautotrophs are extremophiles, but this is not universally so. Different from a lithotroph is an organotroph, an organism which obtains its reducing agents from the catabolism of organic compounds. Carrine Blank mixotroph Wikipedia:Mixotroph A mixotroph is an organism that can use a mix of different sources of energy and carbon. Possible alternations are between photo- and chemotrophy, between litho- and organotrophy, between auto- and heterotrophy or a combination of it. Mixotrophs can be either eukaryotic or prokaryotic. They can take advantage of different environmental conditions. If a trophic mode is obligate, then it is always necessary for sustaining growth and maintenance; if facultative, it can be used as a supplemental source. Some organisms have incomplete Calvin cycles, so they are incapable of fixing carbon dioxide and must use organic carbon sources. Carrine Blank A prokaryote capable of carrying out the role of mixotrophy, where phototrophy and heterotrophy co-occur at the same instance in time. respirer Carrine Blank A prokaryote capable of carrying out the role of respiration, which involves the oxidation and reduction of chemical compounds and involves an electron transport chain. autotrophic A prokaryotic metabolic quality where carbon dioxide is the source of carbon. Carrine Blank phototrophic A prokaryotic metabolic quality where light is the energy source, and the electron donor is an inorganic compound. Carrine Blank chemotrophic A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source. Carrine Blank lithotrophic A prokaryotic metabolic quality where the oxidation and reduction of inorganic chemical compounds is the energy source. Carrine Blank filter paper Crystalline cellulose comprised of microcrystalline or nanocrystalline cellulose fibers, commercially purified and prepared as filter paper. Carrine Blank DSMZ Medium 167 A liquid minerals salt medium containing sodium nitrate, magnesium sulfate heptahydrate, potassium chloride, ferrous sulfate heptahydrate, dipotassium phosphate, potassium dibasic phosphate, yeast extract, and filter paper (a source of pure microcrystalline cellulose). Used for the cultivation of Sporocytophaga. Sporocytophaga medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium167.pdf 167. SPOROCYTOPHAGA MEDIUM NaNO3 2.00 g MgSO4 x 7 H2O 1.00 g KCl 0.50 g FeSO4 x 7 H2O 6.00 mg KH2PO4 0.14 g K2HPO4 1.20 g Yeast extract 0.02 g Distilled water 1000.00 ml Adjust pH to 7.2. Place a strip of sterile filter paper into a culture tube containing 5 ml of medium, thereby leaving 1 - 2 cm of the strip outside of the medium. For DSM 11118 it is recommended to use the following modified medium for the reactivation of freeze-dried samples: Prepare agar plates of medium 167 by supplementing medium with 1.5% (w/v) agar. Place 4 pieces of sterile filter paper on each agar plate. Suspend freeze-dried content of the inner vial of one ampoule with 0.5 ml of liquid medium 167 and inoculate each piece of the filter paper with 1 large drop of the cell suspension. © 2007 DSMZ GmbH - All rights reserved Carrine Blank heterotrophic Carrine Blank A prokaryotic metabolic quality where the carbon and energy sources are organic compounds. mixotrophic A prokaryotic metabolic quality where phototrophy and heterotrophy co-occur at the same instance in time. Carrine Blank chemolithotrophic Carrine Blank A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source, and the electron donor is an inorganic compound. chemolithoautotrophic Carrine Blank A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an inorganic compound, and the carbon source is carbon dioxide. chemolithoheterotrophic Carrine Blank A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an inorganic compound, and the carbon source is an organic compound. chemoorganotrophic A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source, and the electron donor is an organic compound. Carrine Blank chemo-organotrophic chemoorganoautotrophic A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an organic compound, and the carbon source is carbon dioxide. Carrine Blank chemoorganoheterotrophic A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an organic compound, and the carbon source is an organic compound. Carrine Blank photolithotrophic A prokaryotic metabolic quality where light is the energy source, and the electron donor is an inorganic compound. Carrine Blank photolithoautotrophic A prokaryotic metabolic quality where light is the energy source, the electron donor is an inorganic compound, and the carbon source is carbon dioxide Carrine Blank photolithoheterotrophic A prokaryotic metabolic quality where light is the energy source, the electron donor is an inorganic compound, and the carbon source is an organic compound. Carrine Blank photoorganotrophic Carrine Blank A prokaryotic metabolic quality where light is the energy source, and the electron donor is an organic compound. photoorganoautotrophic Carrine Blank A prokaryotic metabolic quality where light is the energy source, the electron donor is an organic compound, and the carbon source is an carbon dioxide. photoorganoheterotrophic A prokaryotic metabolic quality where light is the energy source, the electron donor is an organic compound, and the carbon source is an organic compound. Carrine Blank prokaryotic metabolic quality The metabolic quality of a prokaryotic microorganism. Carrine Blank respiratory metabolic quality Carrine Blank A prokaryotic metabolic quality which involves the oxidation and reduction of chemical compounds and involves an electron transport chain. fermentative metabolic quality A prokaryotic metabolic quality where the carbon and energy sources are organic compounds. Is an anaerobic process which does not require an electron transport system. Carrine Blank fermentative dark fermentation dark fermentative Carrine Blank facultative (photo)heterotroph A prokaryotic metabolic process that involves fermentation in the dark. Is carried out by photosynthetic prokaryotic microorganisms. dark fermentative Carrine Blank A prokaryotic metabolic quality that involves fermentation in the dark. Is carried out by photosynthetic prokaryotic microorganisms. photofermentative Carrine Blank A prokaryotic metabolic quality that involves fermentation in the light. Is carried out by photosynthetic prokaryotic microorganisms. chemotroph Carrine Blank A prokaryote capable of carrying out the role of chemotrophy, where the oxidation and reduction of chemical compounds is the energy source. lithotroph Carrine Blank A prokaryote capable of carrying out the role of lithotrophy, where the oxidation and reduction of inorganic chemical compounds is the energy source. fermenter A prokaryote capable of carrying out the role of fermentation, where the carbon and energy sources are organic compounds. Is an anaerobic process which does not require an electron transport system. Carrine Blank dark fermenter A prokaryote capable of carrying out the role of fermentation in the dark. Is carried out by photosynthetic prokaryotic microorganisms. Carrine Blank photofermenter A prokaryote capable of carrying out the role of fermentation in the light. Is carried out by photosynthetic prokaryotic microorganisms. Carrine Blank chemolithotroph Carrine Blank A prokaryote capable of carrying out the role of chemolithotrophy, where the oxidation and reduction of chemical compounds is the energy source, and the electron donor is an inorganic compound. chemolithoautotroph A prokaryote capable of carrying out the role of chemolithoautotrophy, where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an inorganic compound, and the carbon source is carbon dioxide. Carrine Blank chemolithoheterotroph Carrine Blank A prokaryote capable of carrying out the role of chemolithoheterotrophy, where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an inorganic compound, and the carbon source is an organic compound. chemoorganotroph Carrine Blank A prokaryote capable of carrying out the role of chemoorganotrophy, where the oxidation and reduction of chemical compounds is the energy source, and the electron donor is an organic compound. chemoorganoautotroph Carrine Blank A prokaryote capable of carrying out the role of chemoorganoautotrophy, where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an organic compound, and the carbon source is carbon dioxide. chemoorganoheterotroph Carrine Blank A prokaryote capable of carrying out the role of chemoorganoheterotrophy, where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an organic compound, and the carbon source is an organic compound. photolithotroph A prokaryote capable of carrying out the role of photolithotrophy, where light is the energy source, and the electron donor is an inorganic compound. Carrine Blank photolithoautotroph Carrine Blank A prokaryote capable of carrying out the role of photolithoautotrophy, where light is the energy source, the electron donor is an inorganic compound, and the carbon source is carbon dioxide photolithoheterotroph A prokaryote capable of carrying out the role of photolithoorganotrophy, where light is the energy source, the electron donor is an inorganic compound, and the carbon source is an organic compound. Carrine Blank photoorganotroph A prokaryote capable of carrying out the role of photoorganotrophy, where light is the energy source, and the electron donor is an organic compound. Carrine Blank photoorganoautotroph A prokaryote capable of carrying out the role of photoorganoautotrophy, where light is the energy source, the electron donor is an organic compound, and the carbon source is an carbon dioxide. Carrine Blank photoorganoheterotroph Carrine Blank A prokaryote capable of carrying out the role of photoorganoheterotrophy, where light is the energy source, the electron donor is an organic compound, and the carbon source is an organic compound. (an)aerophile Carrine Blank A prokaryote which is either an aerobe, anaerobe, or facultative anaerobe. aerobe A prokaryote which is capable of growth in the presence of oxygen. Carrine Blank aerobes microaerobe A prokaryote which is capable of growth in the presence of oxygen, where the oxygen concentration is less than atmospheric levels. Carrine Blank obligate aerobe obligately aerobe A prokaryote which is capable of growth in the presence of oxygen. Is incapable of anaerobic growth (growth in the absence of oxygen). Carrine Blank strict aerobe anaerobe A prokaryote which is capable of growth in the absence of oxygen. Carrine Blank anaerobes structurally distinct colony A colony having distinct morphology that manifests in the appearance of the relative position of the parts of the colony. Carrine Blank obligate anaerobe obligately anaerobe Carrine Blank obligately anaerobes A prokaryote which is capable of growth in the absence of oxygen. Cannot grow or live in the presence of oxygen. fastidious anaerobe strict anaerobe obligate anaerobe facultative anaerobe Carrine Blank A prokaryote which is capable of growth in the presence of oxygen or in the absence of oxygen. colony having distinct process quality A prokaryotic colony that has a distinct process quality characteristics. Carrine Blank (an)aerobiosis A prokaryotic metabolic process where a microorganism grows (undergoes cell division) in the presence or absence of oxygen. Carrine Blank facultative anaerobiosis Carrine Blank A prokaryotic metabolic process where the organism is capable of aerobic growth if oxygen is present and is capable of anaerobic growth if oxygen is absent. anaerobiosis A prokaryotic metabolic process where the organism grows in the absence of oxygen. Carrine Blank aerotolerance Carrine Blank A prokaryotic metabolic process where an anaerobic microorganism can grow in the presence of oxygen, but cannot use oxygen as an energy source. obligate anaerobiosis A prokaryotic metabolic process where the organism grows in the absence of oxygen. Cannot grow or live in the presence of oxygen. Carrine Blank aerobiosis A prokaryotic metabolic process where the organism grows in the presence of oxygen. Carrine Blank obligate aerobiosis A prokaryotic metabolic process where the organism grows in the presence of oxygen. Is incapable of anaerobic growth (growth in the absence of oxygen). Uses only oxygen as a terminal electron acceptor. Carrine Blank microaerobiosis A prokaryotic metabolic process where the organism grows in the presence of oxygen, where the oxygen concentration is less than atmospheric levels. Can use oxygen as a terminal electron acceptor through the process of aerobic respiration. Can also have anaerobic metabolic capabilities. Carrine Blank culture medium quality Quality of a microbiological culture medium. Carrine Blank ChEBI terms to be added and processed zzzz *-7 and above labels have not been submitted to ChEBI as new terms yet. The numbers at the end of the temporary class labels help map entities in logical axioms, once the classes have been entered into ChEBI. Carrine Blank obligate psychrophile 20.0 20.0 Carrine Blank A psychrophile which can only grow at depressed temperatures (<20˚C). thermophile 45.0 85.0 45.0 85.0 Prokaryote specialized with respect to temperature, which grows optimally at elevated temperatures (45-85˚C). Carrine Blank hyperthermophile 85.0 85.0 Carrine Blank Prokaryote specialized with respect to temperature, which grows optimally at elevated temperatures (>85˚C). obligate thermophile 45.0 85.0 45.0 85.0 A thermophile which can only grow at elevated temperatures (45-85˚C). Carrine Blank obligate hyperthermophile 85.0 85.0 Carrine Blank A hyperthermophile which can only grow at elevated temperatures (>85˚C). prokaryotic physiologically differentiated cell Carrine Blank Prokaryotic differentiated cell, where the cell is specialized to a particular physical condition with respect to temperature, salinity, or pressure. optimal growth temperature Carrine Blank The optimal growth temperature of a prokaryotic microorganism, which is the temperature at which it undergoes the most rapid pace of growth and cell division. differentiated akinete position relative to heterocytes Cyanobacterial filament where the position of akinetes is differentiated with respect to the physical position of heterocytes. Carrine Blank inorganic chemical sensitivity assay Carrine Blank An assay for the ability of a microorganism to grow in the presence of a potentially growth inhibiting inorganic chemical. pH optimum quality A prokaryotic physiological quality relating the optimal pH of a particular strain of prokaryotes. When these preferred conditions are met, the organism will exhibit cell division. Carrine Blank neutrophilic Carrine Blank pH optimum quality, where growth rates at circumneutral pH values (pH 6-8) are higher than growth rates at other pH values. alkaliphilic Carrine Blank pH optimum quality, where growth rates at moderately alkaline pH values (pH 8-10) are higher than growth rates at other pH values. hyperacidophilic pH optimum quality, where growth rates at highly acidic pH values (pH < 4) are higher than growth rates at other temperatures. Carrine Blank acidophilic pH optimum pH optimum quality, where growth rates at moderately acidic pH values (pH 4-6) are higher than growth rates at other pH values. Carrine Blank hyperalkaliphilic pH optimum quality, where growth rates at highly alkaline pH values (pH >10) are higher than growth rates at other temperatures. Carrine Blank acidotolerant pH optimum quality, where growth rates at circumneutral pH values (pH 6-8) are higher than growth rates at other temperatures, but will tolerate lower pH values. Carrine Blank alkalitolerant pH optimum quality, where growth rates at circumneutral pH values (pH 6-8) are higher than growth rates at other temperatures, but will tolerate higher pH values. Carrine Blank facultative acidophile Carrine Blank Prokaryote specialized with respect to pH, which grows optimally at circumneutral pH values (pH 6-8), but can tolerate lower pH values. facultative alkaliphile Prokaryote specialized with respect to pH, which grows optimally at circumneutral pH values (pH 6-8), but can tolerate higher pH values. Carrine Blank obligately neutrophilic Carrine Blank Neutrophilic, can only grow between pH 6-8. basal cellulolytic medium BC medium From: Robert C, Bernalier-Donadille A. 2003. The cellulolytic microflora of the human colon: evidence of microcrystalline cellulose-degrading bacteria in methane-excreting subjects. FEMS Microbiology Ecology 46:81-89. Cellulolytic bacteria were enumerated in the basal cellulolytic (BC) medium containing, per litre: 200 ml clarified rumen fluid, 75 ml mineral solution I, 75 ml mineral solution II, 10 ml trace element solution [17], 10 ml vitamin solution [18], 10 ml volatile fatty acid solution [18], 0.5 g yeast extract, 1.0 g tryptone, 1 ml resazurin (0.1%), 1 ml hemin (0.1%), 20 ml cysteine sulfide-reducing agent, 2.5 g NaHCO3. Mineral solution I was composed of 6.0 g l31 K2HPO4 and mineral solution II contained, per litre, 6.0 g KH2PO4, 12 g (NH4)2SO4, 12.0 g NaCl, 1.2 g MgSO4, 1.2 g CaCl2. The cysteine sulfide-reducing agent contained 1.25% (w/v) each cysteine-HCl*H2O and Na2S*9H2O. A piece of Whatman No. 1 filter paper cellulose (50 mg) was added to each tube before the addition of the prereduced BC medium (10 ml per tube). After incubation at 37˚C for 21 days, cellulose utilisation in the culture tubes of each dilution inoculated (10^3-10^9) was detected visually by noting degradation of the filter paper. [17] Balch, W.E., Fox, G.E., Magrum, L.J., Woese, C.R. and Wolfe, R.S. (1979) Methanogens: reevaluation of a unique biological group. Microbiol. Rev. 43, 260-296. [18] Scott, H.W. and Dehority, B.A. (1965) Vitamin requirements of several cellulolytic bacteria. J. Bacteriol. 89, 1169-1175. From: Scott HW, Dehority BA. 1965. Vitamin requirements of several cellulolytic rumen bacteria. J Bacteriol 89(5):1169-1175. Volatile Fatty Acids, from Table 1 (mg per 100 mL): Acetic acid 133.0 Isobutyric acid 6.6 Isovaleric acid 8.0 Valeric acid 8.0 A liquid, mineral-salts microbiological culture medium. Used to support the autotrophic growth of cellulolytic bacteria that also require rumen fluid. Contains rumen fluid, minerals salts, Balch trace element solution, vitamins, volatile fatty acids, yeast extract, tryptone, resazurin, hemin, cystine hydrochloride, sodium sulfide, sodium bicarbonate and filter paper (as a cource of cellulose). BCM Carrine Blank Balch trace elements solution Carrine Blank From: Balch WE et al, 1979. Methanogens: reevaluation of a unique biological group. Microbiol Rev 43(2):260. 10 mL trace minerals (pH to 7.0 with KOH; per liter: nitrilotriacetic acid, 1.5 g; MgSO4x7H2O, 3.0 g; MnSO4x2H2O, 0.5 g; NaCl, 1.0 g; FeSO4x7H2O, 0.1g; CoSO4 or CoCl2, 0.1 g; CaCl2x2H2O, 0.1 g; ZnSO4, 0.1 g; CuSO4x5H2O, 0.01 g; AlK(SO4)2, 0.01 g; H3BO3, 0.01 g; Na2MoO4x2H2O, 0.01g); dissolve nitrilotriacetic acid with KOH to pH 6.5 then proceed to add minerals) A trace elements solution containing KOH, nitrilotriacetic acid, magnesium sulfate heptahydrate, manganese sulfate dihydrate, sodium chloride, ferrous sulfate heptahydrate, cobalt sulfate (or cobalt chloride), calcium dichloride dihydrate, zinc sulfate, copper sulfate pentahydrate, aluminum potassium sulfate, boric acid, and disodium molybdate dihydrate. enriched tryptic soy agar A solid, organic rich culture medium based on trytic soy agar. Also contains additional yeast extract, potassium nitrate, sodium lactate, sodium succinate, sodium formate, hemin, menadione (vitamin K3), L-cysteine, dithiothreitol, glucose, sodium fumarate, sodium carbonate, and defibrinated sheep's blood. Prepared under an atmosphere of nitrogen, carbon dioxide, and hydrogen. enriched trypticase soy agar Carrine Blank ETSA ATCC medium 1257 ETSA medium ATCC medium: 1257 ETSA medium Trypticase Soy Agar (BD 211043)...40.0 g Yeast extract......................1.0 g Agar...............................4.0 g KNO3 ...............................0.5 g Sodium lactate, 60% syrup..........1.3 ml Sodium succinate...................0.5 g Sodium formate.....................0.5 g Hemin Solution (see below).........1.0 ml Distilled water..................924.0 ml Autoclave the above solution at 121C for 15 minutes. Cool to 55C. Aseptically add the following, freshly prepared, filter-sterilized solutions in the order listed: Menadione Solution (see below).....2.0 ml 4% L-Cysteine . HCl...............10.0 ml 0.5% Dithiothreitol (DTT).........10.0 ml 10% Glucose.......................10.0 ml 1% Sodium fumarate.................2.0 ml 4% Na2CO3 .........................10.0 ml Defibrinated sheep blood..........30.0 ml This medium solidifies very quickly and should be maintained at 50-55C while dispensing. Aseptically tube the sterile completed medium under an anaerobic atmosphere of 80% N2, 10% CO2, 10% H2. Plug the tubes with butyl rubber stoppers. A note of caution: Hydrogen gas can be explosive in the concentration used in preparing this medium. Gas tanks should be equipped with spark arrestors. Hemin Solution: KOH................................1.12 g 95% Ethanol......................100.0 ml Hemin............................200.0 mg Distilled water..................100.0 ml Dissolve KOH in water. Add ethanol and hemin. Menadione Solution: Menadione (Vitamin K3)............50.0 mg 95% Ethanol.......................50.0 ml Distilled water...................50.0 ml Dissolve menadione in ethanol; then add water. Filter-sterilize solution. optimal growth osmolarity Carrine Blank The optimal growth salinity (osmolarity) of a prokaryotic microorganism, which is the salinity (osmolarity) at which it undergoes the most rapid pace of growth and cell division. optimal growth pH Carrine Blank The optimal growth pH of a prokaryotic microorganism, which is the pH at which it undergoes the most rapid pace of growth and cell division. obligately alkaliphilic Carrine Blank Acialkaliphilic, can only grow between pH 8-10. obligately acidophilic Acidophilic, can only grow between pH 4-6. Carrine Blank obligately hyperalkaliphilic Hyperacialkaliphilic, can only grow above pH 10. Carrine Blank obligately hyperacidophilic Hyperacidophilic, can only grow below pH 4. Carrine Blank obligate neutrophile 6.0 8.0 Carrine Blank Neutrophile, only grows between pH 6-8. defibrinated sheep blood From:https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Sheep Blood, Defibrinated is sheep blood that has been treated to denature fibrinogen without causing cell lysis. It has been shown to be the most accurate blood preparation for the determination of hemolytic activity of streptococci.(3) Sheep blood also contains enzymes that hydrolyze V-factor, thereby inhibiting growth of V-factor dependent Haemophilus haemolyticus which potentially could be misidentified as hemolytic streptococci.(3) Defibrinated sheep blood is free of anticoagulant or preservative. Because washed cell suspensions could result in fragile, hemolyzed blood cells, defibrinated sheep blood is not recommended as a source of washed cells. From: http://medical-dictionary.thefreedictionary.com/defibrinated+blood defibrinated blood - whole blood from which fibrin has been separated during the clotting process. Blood medium ingredient comprised of sheep's (Ovis aries) blood that has been definbrinated (where fibrin has been removed from the blood). Used in the cultivation of microorganisms. Carrine Blank defibrinated sheep's blood anaerobe basal agar Carrine Blank A solid, organic rich microbiological medium for the growth of anaerobes. Contains peptone, yeast extract, sodium chloride, starch, dextrose (D-glucose), sodium pyruvate, arginine, sodium succinate, L-cysteine hydrochloride, sodium bicarbonate, ferric pyrophosphate, hemin, vitamin K, dithiothreitol, and agar. From: http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0972 Typical Formula* gm/litre Peptone 16.0 Yeast extract 7.0 Sodium chloride 5.0 Starch 1.0 Dextrose 1.0 Sodium pyruvate 1.0 Arginine 1.0 Sodium succinate 0.5 L-cysteine HCl 0.25 Sodium bicarbonate 0.4 Ferric pyrophosphate 0.5 Haemin 0.005 Vitamin K 0.0005 Dithiothreitol 0.25 Agar 12.0 pH 6.8 ± 0.2 defibrinated horse blood Carrine Blank Blood medium ingredient comprised of horse's (Equus caballus) blood that has been definbrinated (where fibrin has been removed from the blood). Used in the cultivation of microorganisms. From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Horse Blood, Defibrinated is horse blood that has been treated to denature fibrinogen without causing cell lysis. It is used to supplement blood agar bases. It does not release V-factor hydrolyzing enzymes, and supports the growth of the hemolytic Haemophilus species. Defibrinated horse blood is free of anticoagulant or preservative. Because defibrinated horse blood may result in "incorrect" hemolytic reactions as compared to reactions on sheep blood media, definitive tests are recommended for the differentiation of group D streptococci from group A.(3) PYG medium From: http://www.hopebiol.com/medium/p13HB0398.htm Formula:(g/L) peptone 20.0g glucose 5.0g Baptist yeast powder 10.0g NaCl 0.08g Cysteine hydrochloride 0.5g Calcium chloride 0.008g MgSO4 0.008g K2HPO4 0.04g KH2PO4 0.04g sodium bicarbonate 0.4g PH 7.1-7.3 Carrine Blank PYG PYG broth base An organic-rich, liquid microbiological culture medium containing peptone, glucose, and yeast powder. Also contains minerals salts. yeast powder An undefined organic chemical mixture, derived from deactivated, dehydrated yeast (cells or Saccharomyces cerevisiae). Wikipedia: Nutritional yeast Nutritional yeast is a deactivated yeast, often a strain of Saccharomyces cerevisiae, which is sold commercially as a food product. It is sold in the form of flakes or as a yellow powder and can be found in the bulk aisle of most natural food stores. It is popular with vegans and vegetarians and may be used as an ingredient in recipes or as a condiment.[1] Nutritional yeast is different from yeast extract, which has a very strong flavour and comes in the form of a dark brown paste. Carrine Blank LBM5 medium LBM medium diluted 1 to 5. Carrine Blank alpha-aminobutyric acid assimilation assay a-aminobutyric acid assimilation alpha-aminobutyrate 2-aminobutyrate a-aminobutyrate assimilation 2-aminobutyric acid assimilation alpha-aminobutyrate assimilation Assays for the ability of a microorganism to assimilate alpha-aminobutyrate (alpha-aminobutyric acid) as a sole source of carbon and energy. a-aminobutyric acid alpha-aminobutyric acid Carrine Blank a-aminobutyrate alpha-aminobutyric acid assimilation 2-aminobutyrate assimilation 2-aminobutyric acid poly(ethylene glycol) assimilation assay Carrine Blank PEG Assays for the ability of a microorganism to assimilate poly(ethylene) glycol as a sole source of carbon and energy. polyethylene glycol poly(ethylene) glycol beta-glucuronidase assay with 4-MU MUGLR Carrine Blank An assay for the activity of beta-glucuronidase in a microorganism. Uses the substrate 4-methylumbelliferyl-beta-D-glucuronide. Beta-glucuronidase will cleave the substrate, producing 4-MU (4-methylumbelliferone), which is fluorescent. A positive result is fluorescent; a negative result is not fluorescent. aconitic acid assimilation assay aconitate aconitate assimilation Carrine Blank aconitic acid Assays for the ability of a microorganism to assimilate aconitic acid (aconitate) as a sole source of carbon and energy. aconitic acid assimilation trans-aconitic acid assimilation assay Assays for the ability of a microorganism to assimilate trans-aconitate (trans-aconitic acid) as a sole source of carbon and energy. Carrine Blank transaconitate trans-aconitate trans-aconitate assimilation trans-aconitic acid assimilation trans-aconitic acid beta-galactosidase assay with 4-MU MUGAL An assay for the activity of beta-galactosidase in a microorganism. Uses the substrate 4-methylumbelliferyl-beta-D-galactopyranoside. Beta-galactosidase will cleave the substrate, producing 4-MU (4-methylumbelliferone), which is fluorescent. A positive result is fluorescent; a negative result is not fluorescent. Carrine Blank 6-phospho-beta-galactosidase assay beta-galactosidase 6-phosphate b-PGAL 6-phospho beta-galactosidase beta-PGAL Carrine Blank 6-phospho-b-galactosidase 6-phospho-beta-D-galactoside 6-phosphogalactohydrolase A carbohydrate hydrolysis assay for the activity of 6-phospho-beta-galactosidase in a microorganism which carries out the following reaction: 6-phospho-beta-D-galactoside + H2O <=> 6-phospho-D-galactoside + an alcohol 6-phospho-beta-galactosidase assay with oNP ortho-nitrophenyl-beta-D-galactopyranoside-6-phosphate ONP-beta-D-galactopyranoside-6-phosphate ortho-nitrophenyl-b-D-galactopyranoside-6-phosphate 2-nitrophenyl-b-D-galactopyranoside-6-phosphate oNP-b-D-galactopyranoside-6-phosphate oNP-beta-D-galactopyranoside-6-phosphate 2-nitrophenyl-beta-D-galactopyranoside-6-phosphate An assay for the activity of 6-phospho-beta-galactosidase in a microorganism. Uses the substrate 2-nitrophenyl-beta-D-galactopyranoside-6-phosphate. 6-phospho-beta-galactosidase (beta-galactosidase 6-phosphate) will cleave the substrate, producing 2-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. o-nitrophenyl-beta-D-galactopyranoside-6-phosphate Carrine Blank o-nitrophenyl-b-D-galactopyranoside-6-phosphate alpha-galactosidase assay with 4-MU An assay for the activity of alpha-galactosidase in a microorganism. Uses the substrate 4-methylumbelliferyl-alpha-D-galactopyranoside. Alpha-galactosidase will cleave the substrate, producing 4-MU (4-methylumbelliferone), which is fluorescent. A positive result is fluorescent; a negative result is not fluorescent. 4-MU-a-D-galactoside a-D-GAL 4-MU-alpha-D-galactoside Carrine Blank alpha-D-GAL beta-glucosidase assay with 4-MU 4-MU-b-D-glucoside 4-MU-beta-D-glucoside An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate 4-methylumbelliferyl-beta-D-glucoside. Beta-glucosidase will cleave the substrate, producing 4-MU (4-methylumbelliferone), which is fluorescent. A positive result is fluorescent; a negative result is not fluorescent. Carrine Blank butyrate esterase assay 4-MU-butyrate Carrine Blank Test for butyrate esterase activity in a microorganism using the substrate 4-methylumbelliferyl-butyrate. Esterase that can hydrolyze butyrate will cleave the substrate, producing 4-MU (4-methylumbelliferone), which is fluorescent. A positive result is fluorescent; a negative result is not fluorescent. butyryl-4-MU butyrate esterase activity Carrine Blank A carboxylic ester hydrolase activity, acting on buytrate derivatives. hydrolase assay Carrine Blank A carbohydrate hydrolysis assay, specifically analyzing for the presence of hydrolases (such as lipases or esterases) in a microorganism. DNAse assay with indoxyl A DNse assay with uses 5-bromo-4-chloro-3-indolyl-thymidine-3-phosphate as a substrate. DNase cleaves the substrate, producing 5-bromo-4-chloro-3-hydroxyindole, which is oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, which is intensely blue. A positive result is intensely blue; a negative result is colorless. Carrine Blank DNase assay with pNP Carrine Blank An assay for the activity of DNase in a microorganism. Uses the substrate 4-nitrophenol-thymidine-5-monophosphate. DNase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. thymidine-5-monophosphate-PNP ester prokaryotic cultured clonal cell population Carrine Blank A cultured clonal cell population comprised of prokaryotic cells. Could be manifested as a colony or a liquid culture derived from a single individual (a homogenous population founded by a single individual, or clone). indoxyl acetate hydrolysis assay Carrine Blank indoxyl acetate reaction An assay for bacterial hydrolytic assays which use indoxyl acetate as a substrate. In the presence of oxygen, released indoxyl will turn an indigo color indicating a positive reaction. Assays for the following reaction: Indoxyl acetate + H2O <=> Indoxyl + acetic acid culture tube Carrine Blank A test tube used to culture a microorganism. petri dish A device used to culture microorganisms on solid microbiological culture medium. Carrine Blank prokaryotic clonal liquid culture A prokaryotic cultured clonal cell population housed in a test tube which uses a culture tube with liquid culture medium as a substrate to support growth. Carrine Blank RapID 32 system Carrine Blank RapID™ ANA II System, a set of specialized identification kits that contain 32 reagents designed to identify particular types of microorganisms. fildes enrichment Blood medium ingredient comprised of a peptic digest of sheep (Ovis aries) or horse (Equus caballus) blood, serves as a source of hemin and NAD (nicotinamide adenine dinucleotide). Used in the cultivation of microorganisms. From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Fildes Enrichment is a peptic digest of sheep or horse blood that serves as a rich source of growth factors including hemin (X-factor) and nicotinamide adenine dinucleotide (V-factor). Carrine Blank hemin solution From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Hemin Solution is a mixture of hemin and NaOH. Carrine Blank A defined organic solution comprised of a solution of hemin (derived from blood) and sodium hydroxide. Used in the cultivation of microorganisms. dried bovine hemoglobin A blood medium ingredient comprised of bovine (Bos taurus) hemoglobin that has been dried (i.e. has a decreased water content), providing a source of hemin. Used in the cultivation of microorganisms. Carrine Blank From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Hemoglobin, Dried Bovine is used to provide hemin required by many fastidious microorganisms. hemoglobin solution From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Hemoglobin Solution, 2% provides hemin required by many fastidious microorganisms. A defined organic solution comprised of hemoglobin, providing a source of hemin. Used in the cultivation of microorganisms. Carrine Blank oxalated horse blood Blood medium ingredient comprised of horse (Equus caballus) blood, where potassium oxalate has been added to prevent coagulation. Used in the cultivation of microorganisms. Carrine Blank From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Horse Blood, Oxalated is horse blood that has been treated with potassium oxalate as an anticoagulant. horse blood with heparin Blood medium ingredient comprised of horse (Equus caballus) blood where heparin has been added to prevent coagulation. Used in the cultivation of microorganisms. Carrine Blank From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Horse Blood, with Heparin is whole horse blood treated with the anticoagulant heparin. Heparin-binding of Mycobacterium tuberculosis has been suggested to increase the organism's virulence in primary infection and in extrapulmonary dissemination(5). DSMZ Medium 1 NA An organic-rich, solid microbiological culture medium comprised of peptone, meat extract, and agar. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1.pdf 1. NUTRIENT AGAR Peptone 5.0 g Meat extract 3.0 g Agar, if necessary 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. © 2007 DSMZ GmbH - All rights reserved nutrient agar DSM strains: 29601 Achromobacter animicus nutrient agar medium NA agar DSMZ Medium 10 Carrine Blank Zymonomas medium An organic-rich, solid microbiological culture medium containing Bacto peptone, yeast extract and glucose. Used for the cultivation of Zymomonas. DSM strains: Zymomonas agar http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium10.pdf 10. ZYMOMONAS MEDIUM Bacto peptone 10.0 g Yeast extract 10.0 g Glucose 20.0 g Agar if required 15.0 g Distilled water 1000.0 ml © 2012 DSMZ GmbH - All rights reserved DSMZ Medium 101 agar An organic-rich, solid microbiological culture medium containing beef extract, peptone and sodium chloride (30g/L). Used for the cultivation of general heterotrophic microorganisms. Carrine Blank nutrient agar with sodium chloride nutrient agar with NaCl DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium101.pdf 101. NUTRIENT AGAR or BROTH WITH NaCl Peptone 5.0 g Meat extract 3.0 g NaCl 30.0 g Agar, if necessary 15.0 g Distilled water 1000.0 ml © 2010 DSMZ GmbH - All rights reserved DSMZ Medium 101 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium101.pdf 101. NUTRIENT AGAR or BROTH WITH NaCl Peptone 5.0 g Meat extract 3.0 g NaCl 30.0 g Agar, if necessary 15.0 g Distilled water 1000.0 ml © 2010 DSMZ GmbH - All rights reserved Carrine Blank nutrient broth medium with NaCl An organic-rich, liquid microbiological culture medium containing beef extract, peptone and sodium chloride (30g/L). Used for the cultivation of general heterotrophic microorganisms. nutrient broth with NaCl nutrient broth with sodium chloride DSM strains: DSMZ Medium 102 Flavobacterium aquatile medium An organic-rich, solid microbiological culture medium containing sodium caseinate, yeast extract, proteose peptone, potassium phosphate, and agar. Used for the cultivation of Flavobacterium aquatile. Carrine Blank Flavobacterium aquatile agar http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium102.pdf 102. FLAVOBACTERIUM AQUATILE MEDIUM Na-caseinate 2.0 g Yeast extract 0.5 g Proteose peptone 1.0 g K2HPO4 0.5 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.4. © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 1000 A minerals-salts, liquid culture medium comprised of sodium chloride, potassium phosphate, calcium chloride, ammonium chloride, magnesium sulfate, magnesium chloride, potassium chloride, ferric sulfate, nickel chloride, sotium selenate, sodium silicate, and trace minerals. Prepared under at atmosphere of hydrogen and carbon dioxide. Mjanhox-nitrate medium with supplement Mjanhox-NO3 medium with supplement Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1000.pdf 1000. MJANHOX-NO3 MEDIUM WITH SUPPLEMENT NaCl 3.000 g K2HPO4 0.014 g CaCl2 x 2 H2O 0.014 g NH4Cl 0.040 g MgSO4 x 7 H2O 0.340 g MgCl2 x 6 H20 0.418 g KCl 0.033 g Fe2(SO4)3 x X H2O 0.005 g NiCl2 x 6 H2O 0.005 mg Na2SeO3 x 5 H2O 0.005 mg Na2SiO3 x 9 H20 0.500 g Trace mineral solution (see below) 1.000 ml Distilled water 1000.000 ml Before autoclaving, the pH of the Medium is adjusted with NaOH to 7.5-8.0. After autoclaving, the filter-sterilized vitamin solution (0.1% volume), and each of the separately autoclaved, concentrated solutions are added to the medium at a final concentration described below. Then a mix gas (80% H2, 20% CO2) is purged for 5 min. Finally, the mix gas is compressed into gas phase (> 80% volume of the tube or bottle) at 3 atm. Na2S2O3 x 5 H2O 10 mM NaHCO3 0.20 % NaNO3 0.05 % Trace mineral solution: nitrilotriacetic acid 1.500 g MnSO4 x 2 H2O 0.500 g CoSO4 x 7 H2O 0.500 g ZnSO4 x 7 H2O 0.180 g CuSO4 x 5 H2O 0.010 g KAl(SO4)2 x 12 H2O 0.020 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.010 g © 2007 DSMZ GmbH - All rights reserved trace mineral solution for DSMZ Medium 1000 Carrine Blank A trace elements solution containing nitrilotriacetic acid, manganese sulfate, cobalt sulfate, zinc sulfate, copper sulfate, potassium aluminum sulfate, boric acid, and sodium molybdate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1000.pdf 1000. MJANHOX-NO3 MEDIUM WITH SUPPLEMENT NaCl 3.000 g K2HPO4 0.014 g CaCl2 x 2 H2O 0.014 g NH4Cl 0.040 g MgSO4 x 7 H2O 0.340 g MgCl2 x 6 H20 0.418 g KCl 0.033 g Fe2(SO4)3 x X H2O 0.005 g NiCl2 x 6 H2O 0.005 mg Na2SeO3 x 5 H2O 0.005 mg Na2SiO3 x 9 H20 0.500 g Trace mineral solution (see below) 1.000 ml Distilled water 1000.000 ml Before autoclaving, the pH of the Medium is adjusted with NaOH to 7.5-8.0. After autoclaving, the filter-sterilized vitamin solution (0.1% volume), and each of the separately autoclaved, concentrated solutions are added to the medium at a final concentration described below. Then a mix gas (80% H2, 20% CO2) is purged for 5 min. Finally, the mix gas is compressed into gas phase (> 80% volume of the tube or bottle) at 3 atm. Na2S2O3 x 5 H2O 10 mM NaHCO3 0.20 % NaNO3 0.05 % Trace mineral solution: nitrilotriacetic acid 1.500 g MnSO4 x 2 H2O 0.500 g CoSO4 x 7 H2O 0.500 g ZnSO4 x 7 H2O 0.180 g CuSO4 x 5 H2O 0.010 g KAl(SO4)2 x 12 H2O 0.020 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.010 g © 2007 DSMZ GmbH - All rights reserved sodium caseinate Undefined mixtures of complex organic compounds derived from the milk proteins (casein) of a mammal, where the casein has been complexed with sodium. Used in the cultivation of microorganisms. Na-caseinate Carrine Blank DSMZ Medium 1001 A minerals-salts, liquid culture medium comprised of sodium bicarbonate, ammonium chloride, sodium phosphate, potassium chloride, vitamin mix, and mineral mix. Prepared under an atmosphere of dinitrogen and carbon dioxide. Sodium acetate and iron-NTA are then added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1001.pdf 1001. BASAL MEDIUM NaHCO3 2.500 g NH4Cl 0.250 g NaH2PO4 x H2O 0.600 g KCl 0.100 g Vitamin Mix (see below) 10.000 ml Mineral Mix (see below) 10.000 ml Distilled water 980.000 ml This medium should not be exposed to direct sunlight! Add all basal medium ingredients. Bring the final volume of the medium to 1.0 liter. Dispense to appropriate culture containers. Bubble the medium with 80:20 N2:CO2 (final pH sould be 6.8 to 7.0) - approximately 10 ml of media (anaerobic culture tube) should be gassed for 5 min in the aqueous phase (bubbled) and the headspace gassed for one minute prior to sealing the container. Sterilize per usual procedure. Add electron donor (Acetate-final conc. of 10 mM-recipe below) and electron acceptor (Fe(III)NTA-final conc. of 10 mM-recipe below) from sterile, anaerobic stock solutions using a sterile syringe and needle flushed with anaerobic gas. Store out of direct light. 1.0 M Acetate Stock Solution: Add 13.6 g sodium acetate to ca. 80 ml distilled water. Bring to final volume of 100 ml. Bubble with anaerobic gas (nitrogen) for 45 min. Seal and sterilize. 500 mM Fe(III)NTA Stock solution: Add 8.2 g NaHCO3 to ca. 70 ml distilled water. Add 12.8 g of Na3Nitrilotriacetic acid (NTA) to ca. 70 ml distilled water. Add 13.5 g FeCl3 x 6 H2O to ca 70 ml distilled water. Adjust pH to 6.5 using 10N NaOH. Bring solution to final volume of 100 ml. Ingredients will go into solution after stirring for ca. 15 min. Bubble with anaerobic gas (N2) for 45 min and then filter sterilize (0.2 μm filter) into a sterile, anaerobic, serum bottle. Vitamin mixture: Biotin 2.000 mg Folic acid 2.000 mg Pyridoxine HCl 10.000 mg Riboflavin 5.000 mg Thiamine 5.000 mg Nicotinic acid 5.000 mg Pantothenic acid 5.000 mg Vitamin B12 0.100 mg p-aminobenzoic acid 5.000 mg Thioctic acid 5.000 mg Distilled water 1000.000 ml Mineral mixture: NTA 1.500 g MgSO4 3.000 g MnSO4 x H2O 0.500 g NaCl 1.000 g FeSO4 x 7 H2O 0.100 g CaCl2 x 2 H2O 0.100 g CoCl2 x 6 H2O 0.100 g ZnCl2 0.130 g CuSO4 x 5 H2O 0.010 g AlK(SO4)2 x 12 H2O 0.010 g H3BO3 0.010 g Na2MoO4 0.025 g NiCl2 x 6 H2O 0.024 g Na2WO4 x 2 H2O 0.025 g Distilled water 1000.000 ml © 2007 DSMZ GmbH - All rights reserved DSM strains: basal medium Carrine Blank basal medium mineral mixture http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1001.pdf 1001. BASAL MEDIUM NaHCO3 2.500 g NH4Cl 0.250 g NaH2PO4 x H2O 0.600 g KCl 0.100 g Vitamin Mix (see below) 10.000 ml Mineral Mix (see below) 10.000 ml Distilled water 980.000 ml This medium should not be exposed to direct sunlight! Add all basal medium ingredients. Bring the final volume of the medium to 1.0 liter. Dispense to appropriate culture containers. Bubble the medium with 80:20 N2:CO2 (final pH sould be 6.8 to 7.0) - approximately 10 ml of media (anaerobic culture tube) should be gassed for 5 min in the aqueous phase (bubbled) and the headspace gassed for one minute prior to sealing the container. Sterilize per usual procedure. Add electron donor (Acetate-final conc. of 10 mM-recipe below) and electron acceptor (Fe(III)NTA-final conc. of 10 mM-recipe below) from sterile, anaerobic stock solutions using a sterile syringe and needle flushed with anaerobic gas. Store out of direct light. 1.0 M Acetate Stock Solution: Add 13.6 g sodium acetate to ca. 80 ml distilled water. Bring to final volume of 100 ml. Bubble with anaerobic gas (nitrogen) for 45 min. Seal and sterilize. 500 mM Fe(III)NTA Stock solution: Add 8.2 g NaHCO3 to ca. 70 ml distilled water. Add 12.8 g of Na3Nitrilotriacetic acid (NTA) to ca. 70 ml distilled water. Add 13.5 g FeCl3 x 6 H2O to ca 70 ml distilled water. Adjust pH to 6.5 using 10N NaOH. Bring solution to final volume of 100 ml. Ingredients will go into solution after stirring for ca. 15 min. Bubble with anaerobic gas (N2) for 45 min and then filter sterilize (0.2 μm filter) into a sterile, anaerobic, serum bottle. Vitamin mixture: Biotin 2.000 mg Folic acid 2.000 mg Pyridoxine HCl 10.000 mg Riboflavin 5.000 mg Thiamine 5.000 mg Nicotinic acid 5.000 mg Pantothenic acid 5.000 mg Vitamin B12 0.100 mg p-aminobenzoic acid 5.000 mg Thioctic acid 5.000 mg Distilled water 1000.000 ml Mineral mixture: NTA 1.500 g MgSO4 3.000 g MnSO4 x H2O 0.500 g NaCl 1.000 g FeSO4 x 7 H2O 0.100 g CaCl2 x 2 H2O 0.100 g CoCl2 x 6 H2O 0.100 g ZnCl2 0.130 g CuSO4 x 5 H2O 0.010 g AlK(SO4)2 x 12 H2O 0.010 g H3BO3 0.010 g Na2MoO4 0.025 g NiCl2 x 6 H2O 0.024 g Na2WO4 x 2 H2O 0.025 g Distilled water 1000.000 ml © 2007 DSMZ GmbH - All rights reserved Carrine Blank A trace elements solution containing nitrilotriacetic acid, magnesium sulfate, manganese sulfate, sodium chloride, ferrous sulfate, calcium chloride, cobalt chloride, zinc chloride, copper sulfate, potassium aluminum sulfate, boric acid, sodium molybdate, nickel chloride, and sodium tungstate. basal medium vitamin mixture Carrine Blank A microbiological vitamin solution containing biotin, folic acid, pyridoxine hydrochloride, riboflavin, thiamine, nicotinic acid, pantothenic acid, vitamin B12 (cobalamin), p-aminobenzoic acid (4-aminobenzoic acid), and thioctic acid (lipoic acid). http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1001.pdf 1001. BASAL MEDIUM NaHCO3 2.500 g NH4Cl 0.250 g NaH2PO4 x H2O 0.600 g KCl 0.100 g Vitamin Mix (see below) 10.000 ml Mineral Mix (see below) 10.000 ml Distilled water 980.000 ml This medium should not be exposed to direct sunlight! Add all basal medium ingredients. Bring the final volume of the medium to 1.0 liter. Dispense to appropriate culture containers. Bubble the medium with 80:20 N2:CO2 (final pH sould be 6.8 to 7.0) - approximately 10 ml of media (anaerobic culture tube) should be gassed for 5 min in the aqueous phase (bubbled) and the headspace gassed for one minute prior to sealing the container. Sterilize per usual procedure. Add electron donor (Acetate-final conc. of 10 mM-recipe below) and electron acceptor (Fe(III)NTA-final conc. of 10 mM-recipe below) from sterile, anaerobic stock solutions using a sterile syringe and needle flushed with anaerobic gas. Store out of direct light. 1.0 M Acetate Stock Solution: Add 13.6 g sodium acetate to ca. 80 ml distilled water. Bring to final volume of 100 ml. Bubble with anaerobic gas (nitrogen) for 45 min. Seal and sterilize. 500 mM Fe(III)NTA Stock solution: Add 8.2 g NaHCO3 to ca. 70 ml distilled water. Add 12.8 g of Na3Nitrilotriacetic acid (NTA) to ca. 70 ml distilled water. Add 13.5 g FeCl3 x 6 H2O to ca 70 ml distilled water. Adjust pH to 6.5 using 10N NaOH. Bring solution to final volume of 100 ml. Ingredients will go into solution after stirring for ca. 15 min. Bubble with anaerobic gas (N2) for 45 min and then filter sterilize (0.2 μm filter) into a sterile, anaerobic, serum bottle. Vitamin mixture: Biotin 2.000 mg Folic acid 2.000 mg Pyridoxine HCl 10.000 mg Riboflavin 5.000 mg Thiamine 5.000 mg Nicotinic acid 5.000 mg Pantothenic acid 5.000 mg Vitamin B12 0.100 mg p-aminobenzoic acid 5.000 mg Thioctic acid 5.000 mg Distilled water 1000.000 ml Mineral mixture: NTA 1.500 g MgSO4 3.000 g MnSO4 x H2O 0.500 g NaCl 1.000 g FeSO4 x 7 H2O 0.100 g CaCl2 x 2 H2O 0.100 g CoCl2 x 6 H2O 0.100 g ZnCl2 0.130 g CuSO4 x 5 H2O 0.010 g AlK(SO4)2 x 12 H2O 0.010 g H3BO3 0.010 g Na2MoO4 0.025 g NiCl2 x 6 H2O 0.024 g Na2WO4 x 2 H2O 0.025 g Distilled water 1000.000 ml © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 220 DSM strains: Carrine Blank From: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium220.pdf 220. CASO AGAR (Merck 105458) Peptone from casein 15.0 g Peptone from soymeal 5.0 g NaCl 5.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid Cm131). For strain DSM 21449 und DSM 21450 NaCl 10.0 g/L MnSO4 10.0 mg/L For strain DSM 5746 Methanol © 2007 DSMZ GmbH - All rights reserved CASO agar An organic-rich, solid medium containing casitone (pancreatic digest of casein), Phytone peptone (papaic digest of soybean), and sodium chloride. Used for the cultivation of aerobic heterotrophic microorganisms. DSMZ Medium 1002 DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1002.pdf 1002. ECTOTHIORHODOSYNUS MEDIUM KH2PO4 0.5 g NH4Cl 0.5 g NaCl 20.0 g MgCl2 x 6 H2O 0.2 g CaCl2 0.1 g Na2CO3 5.0 g NaHCO3 5.0 g Sodium acetate 1.0 g Yeast extract 0.1 g Na2S2O3 x 5H2O 0.5 g Na2S x 9 H2O 0.1 g Trace elements SL6 (see medium 27) 1.0 ml Vitamin B12 20.0 μg Distilled water 1000.0 ml Prepare the NH4Cl, CaCl2, carbonate, bicarbonate and sulfide as a single sterile stock solution and add to the sterile medium after autoclaving. The pH should be adjust to 9-9.5. © 2007 DSMZ GmbH - All rights reserved Ectothiorhodosynus medium A minerals-salts, liquid microbiological culture medium comprised of sodium acetate, yeast extract, sodium thiosulfate, and sodium sulfide. Used for the cultivation of Ectothiorhodosynus. trace elements solution SL-6 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium27.pdf 27. RHODOSPIRILLACEAE MEDIUM (modified) Yeast extract 0.30 g Na2-succinate 1.00 g (NH4)-acetate 0.50 g Fe(III) citrate solution (0.1% in H2O) 5.00 ml KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g Vitamin B12 solution (10 mg in 100 ml H2O) 0.40 ml Trace element solution SL-6 (see below) 1.00 ml L-Cysteiniumchloride 0.30 g Resazurin(0,1%) 0.50 ml Distilled water 1000.00 ml Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121˚C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml © 2007 DSMZ GmbH - All rights reserved Carrine Blank A trace elements solution containing zinc sulfate, manganese chloride, boric acid, cobalt chloride, copper chloride, nickel chloride, and sodium molybdate. DSMZ Medium 27 A minerals-salts, mildly reducing liquid microbiological culture medium used for the cultivation of Rhodospirillaceae. Contains yeast extract, sodium succinate, ammonium acetate, iron citrate and cysteinium chloride as a reducing agent. Prepared under an atmosphere of dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium27.pdf 27. RHODOSPIRILLACEAE MEDIUM (modified) Yeast extract 0.30 g Na2-succinate 1.00 g (NH4)-acetate 0.50 g Fe(III) citrate solution (0.1% in H2O) 5.00 ml KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g Vitamin B12 solution (10 mg in 100 ml H2O) 0.40 ml Trace element solution SL-6 (see below) 1.00 ml L-Cysteinium chloride 0.30 g Resazurin(0,1%) 0.50 ml Distilled water 1000.00 ml Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121˚C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml © 2007 DSMZ GmbH - All rights reserved Rhodospirillaceae medium (modified) Carrine Blank DSM strains: DSMZ Medium 1003 hydrogen-oxidizing medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1003.pdf 1003. HYDROGEN-OXYDIZING MEDIUM Place 1000 ml of anaerobic water (CO2 water) in a flask. Add the following compounds: MgSO4 x 7 H2O 7.00 g NaS2O3 2.00 g CaCl2 x 2 H2O 0.40 g KCl 0.48 g MgCl2 0.78 g MES 1.95 g Add solutions A, B, and D (see recipes below): Solution A 2.00 ml Solution B 1.50 ml Solution D (Trace minerals) 10.00 ml pH the media to 6.0 and gas the flask containing the media with CO2 for 20 min at least. Gas first culture tube in rack and place 5 ml of media in previous tube. Place stopper on loaded culture tube, crimp tube cap onto stopper. Autoclave the medium for 20 min., 121˚C. Add 1 ml of O2 to each tube before inoculation. After the inoculation the tubes are pressurized with H2 (138 KP). Solution A (100x solution): NH4Cl 100.00 g MgCl2 x H2O 100.00 g CaCl2 x 2 H2O 40.00 g Distilled water 1000.00 ml Adjust the pH to 4 with HCl. Solution B (500x solution): K2HPO4 x 3 H2O 200.00 g Distilled water 1000.00 ml Trace mineral solution (100x): see next page Trace mineral solution (100x): Na-EDTA x 2 H2O 500.00 mg CoCl2 x 6 H2O 150.00 mg MnCl2 x 4 H2O 100.00 mg FeSO4 x 7 H2O 100.00 mg ZnCl2 100.00 mg AlCl3 x 6 H2O 40.00 mg Na-tungstate x 2 H2O 30.00 mg CuCl 20.00 mg Ni2SO4 x 6 H2O 20.00 mg Se-acid 10.00 mg HBO3 10.00 mg Na2MoO4 x 2 H2O 10.00 mg Distilled water 1000.00 ml Adjust the pH of the solution to 3.0 with HCl. © 2007 DSMZ GmbH - All rights reserved A minerals-salts, liquid medium containing magnesium sulfate, sodium thiosulfate, MES, hydrogen and oxygen. Used to support the growth of slighly acidophilic hydrogen-oxidizing bacteria. Carrine Blank trace mineral solution for DSMZ Medium 1003 A trace elements solution containing sodium EDTA, cobalt chloride, manganese chloride, ferrous sulfate zinc chloride, aluminum trichloride, sodium tungstate, copper chloride, nickel sulfate, selenic acid (se-acid), boric acid, and sodium molybdate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1003.pdf 1003. HYDROGEN-OXYDIZING MEDIUM Place 1000 ml of anaerobic water (CO2 water) in a flask. Add the following compounds: MgSO4 x 7 H2O 7.00 g NaS2O3 2.00 g CaCl2 x 2 H2O 0.40 g KCl 0.48 g MgCl2 0.78 g MES 1.95 g Add solutions A, B, and D (see recipes below): Solution A 2.00 ml Solution B 1.50 ml Solution D (Trace minerals) 10.00 ml pH the media to 6.0 and gas the flask containing the media with CO2 for 20 min at least. Gas first culture tube in rack and place 5 ml of media in previous tube. Place stopper on loaded culture tube, crimp tube cap onto stopper. Autoclave the medium for 20 min., 121˚C. Add 1 ml of O2 to each tube before inoculation. After the inoculation the tubes are pressurized with H2 (138 KP). Solution A (100x solution): NH4Cl 100.00 g MgCl2 x H2O 100.00 g CaCl2 x 2 H2O 40.00 g Distilled water 1000.00 ml Adjust the pH to 4 with HCl. Solution B (500x solution): K2HPO4 x 3 H2O 200.00 g Distilled water 1000.00 ml Trace mineral solution (100x): see next page Trace mineral solution (100x): Na-EDTA x 2 H2O 500.00 mg CoCl2 x 6 H2O 150.00 mg MnCl2 x 4 H2O 100.00 mg FeSO4 x 7 H2O 100.00 mg ZnCl2 100.00 mg AlCl3 x 6 H2O 40.00 mg Na-tungstate x 2 H2O 30.00 mg CuCl 20.00 mg Ni2SO4 x 6 H2O 20.00 mg Se-acid 10.00 mg HBO3 10.00 mg Na2MoO4 x 2 H2O 10.00 mg Distilled water 1000.00 ml Adjust the pH of the solution to 3.0 with HCl. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 1004 A minerals-salts liquid medium containing potassium phosphate, magnesium chloride, calcium chloride, ammonium chloride, trace elements, selenite-tungstate solution, yeast extract, resazurin, sodium bicarbonate, vitamin solution, D-glucose, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Used to support the growth of Anaerolinea. DSM strains: Anaerolinea medium Carrine Blank DSM strains: 16554 Anaerolinea thermolimosa 14523 Anaerolinea thermophila UNI-1 17877 Bellilinea caldifistulae 14535 Caldilinea aerophila DSM 14535 = NBRC 104270 22659 Caldilinea tarbellica 16556 Leptolinea tardivitalis 16555 Levilinea saccharolytica 23815 Ornatilinea apprima http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1004.pdf 1004. ANAEROLINEA MEDIUM KH2PO4 0.14 g MgCl2 x 6 H2O 0.20 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.54 g Trace element solution SL-11 (see medium 784) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.30 g Resazurin 1.00 mg NaHCO3 2.50 g Vitamins solution (see medium 141) 10.00 ml D-Glucose 2.20 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, glucose and reducing agents), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add solid bicarbonate and adjust pH to 7.0. Dispense under same gas atmosphere in culture vessels and autoclave. Add glucose, vitamins (sterilized by filtration), sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. The final pH should be 7.0. For DSM 16554, DSM 16555 and DSM 17877 replace glucose with 7.20 g/l sucrose and reduce amount of yeast extract to 0.10 g/l. For DSM 16556 omit glucose and reduce amount of yeast extract to 0.10 g/l. For DSM 22659 increase amount of glucose to 5.00 g/l. For DSM 23815 replace glucose with 2.00 g/l cellobiose added after autoclaving from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 1004.1 Carrine Blank DSMZ Medium 1004.1 -< for DSM 16554, DSM 16555, and DSM 17877 A minerals-salts liquid medium similar to DSMZ Medium 1004, except that glucose is omitted, sucrose is added, and the amount of yeast extract is reduced. DSM 16554 is Anaerolinea thermolimosa DSM 16555 is Levilinea saccharolytica DSM 17877 is Bellilinea caldifistulae http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1004.pdf 1004. ANAEROLINEA MEDIUM KH2PO4 0.14 g MgCl2 x 6 H2O 0.20 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.54 g Trace element solution SL-11 (see medium 784) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.30 g Resazurin 1.00 mg NaHCO3 2.50 g Vitamins solution (see medium 141) 10.00 ml D-Glucose 2.20 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, glucose and reducing agents), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add solid bicarbonate and adjust pH to 7.0. Dispense under same gas atmosphere in culture vessels and autoclave. Add glucose, vitamins (sterilized by filtration), sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. The final pH should be 7.0. For DSM 16554, DSM 16555 and DSM 17877 replace glucose with 7.20 g/l sucrose and reduce amount of yeast extract to 0.10 g/l. For DSM 16556 omit glucose and reduce amount of yeast extract to 0.10 g/l. For DSM 22659 increase amount of glucose to 5.00 g/l. For DSM 23815 replace glucose with 2.00 g/l cellobiose added after autoclaving from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 1004.1 DSMZ Medium 1004.2 A minerals-salts liquid medium similar to DSMZ Medium 1004, except that glucose is omitted and the amount of yeast extract is reduced. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1004.pdf 1004. ANAEROLINEA MEDIUM KH2PO4 0.14 g MgCl2 x 6 H2O 0.20 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.54 g Trace element solution SL-11 (see medium 784) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.30 g Resazurin 1.00 mg NaHCO3 2.50 g Vitamins solution (see medium 141) 10.00 ml D-Glucose 2.20 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, glucose and reducing agents), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add solid bicarbonate and adjust pH to 7.0. Dispense under same gas atmosphere in culture vessels and autoclave. Add glucose, vitamins (sterilized by filtration), sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. The final pH should be 7.0. For DSM 16554, DSM 16555 and DSM 17877 replace glucose with 7.20 g/l sucrose and reduce amount of yeast extract to 0.10 g/l. For DSM 16556 omit glucose and reduce amount of yeast extract to 0.10 g/l. For DSM 22659 increase amount of glucose to 5.00 g/l. For DSM 23815 replace glucose with 2.00 g/l cellobiose added after autoclaving from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 1004.2 -< for DSM 16556 DSM 16556 is Leptolinea tardivitalis DSMZ Medium 1004.2 Carrine Blank DSMZ Medium 1004.3 DSM 22659 is Caldilinea tarbellica Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1004.pdf 1004. ANAEROLINEA MEDIUM KH2PO4 0.14 g MgCl2 x 6 H2O 0.20 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.54 g Trace element solution SL-11 (see medium 784) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.30 g Resazurin 1.00 mg NaHCO3 2.50 g Vitamins solution (see medium 141) 10.00 ml D-Glucose 2.20 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, glucose and reducing agents), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add solid bicarbonate and adjust pH to 7.0. Dispense under same gas atmosphere in culture vessels and autoclave. Add glucose, vitamins (sterilized by filtration), sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. The final pH should be 7.0. For DSM 16554, DSM 16555 and DSM 17877 replace glucose with 7.20 g/l sucrose and reduce amount of yeast extract to 0.10 g/l. For DSM 16556 omit glucose and reduce amount of yeast extract to 0.10 g/l. For DSM 22659 increase amount of glucose to 5.00 g/l. For DSM 23815 replace glucose with 2.00 g/l cellobiose added after autoclaving from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 1004.3 A minerals-salts liquid medium similar to DSMZ Medium 1004, except that the amount of glucose is increased. DSMZ Medium 1004.3 -< for DSM 22659 DSMZ Medium 1004.4 DSM 23815 is Ornatilinea apprima DSMZ Medium 1004.4 A minerals-salts liquid medium similar to DSMZ Medium 1004, except that glucose is omitted and cellobiose added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1004.pdf 1004. ANAEROLINEA MEDIUM KH2PO4 0.14 g MgCl2 x 6 H2O 0.20 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.54 g Trace element solution SL-11 (see medium 784) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.30 g Resazurin 1.00 mg NaHCO3 2.50 g Vitamins solution (see medium 141) 10.00 ml D-Glucose 2.20 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, glucose and reducing agents), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add solid bicarbonate and adjust pH to 7.0. Dispense under same gas atmosphere in culture vessels and autoclave. Add glucose, vitamins (sterilized by filtration), sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. The final pH should be 7.0. For DSM 16554, DSM 16555 and DSM 17877 replace glucose with 7.20 g/l sucrose and reduce amount of yeast extract to 0.10 g/l. For DSM 16556 omit glucose and reduce amount of yeast extract to 0.10 g/l. For DSM 22659 increase amount of glucose to 5.00 g/l. For DSM 23815 replace glucose with 2.00 g/l cellobiose added after autoclaving from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 1004.4 -< for DSM 23815 Carrine Blank DSMZ Medium 784 Natroniella medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium784.pdf 784. NATRONIELLA MEDIUM KH2PO4 0.2 g MgCl2 x 6 H2O 0.1 g NH4Cl 1.0 g KCl 0.2 g NaCl 16.0 g Trace element solution SL-11 (see below) 1.0 ml Yeast extract 0.2 g Resazurin 0.5 mg Na2CO3 68.0 g NaHCO3 38.0 g Ethanol 5.0 ml Vitamin solution (see medium 141) 10.0 ml Na2S x 9 H2O 1.0 g Distilled water 1000.0 ml Trace element solution SL-11: Na2-EDTA 5.2 g FeCl2 x 4 H2O 1.5 g ZnCl2 70.0 mg MnCl2 x 4 H2O 100.0 mg H3BO3 6.0 mg CoCl2 x 6 H2O 190.0 mg CuCl2 x 2 H2O 2.0 mg NiCl2 x 6 H2O 24.0 mg Na2Mo4 x 2 H2O 36.0 mg Distilled water 1000.0 ml Adjust pH of solution to 6.0. Dissolve ingredients except carbonates, ethanol, vitamins and sulfide. Bring medium to the boil, then cool to room temperature while flushing with 100% N2 gas. Add and dissolve carbonates and sulfide while gassing the head space only. Dispense and autoclave under N2 gas atmosphere. Before use add ethanol and vitamins from sterile anoxic stock solution prepared under N2. Vitamins are sterilized by filtration. Adjust pH of the completed medium to 9.5 – 10.0. For DSM 24923 omit ethanol and add to the autoclaved medium 2.0 g/l Na2S2O3 x 5 H2O from a sterile anoxic stock solution. © 2013 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: 24912 Natranaerobaculum magadiense 9952 Natroniella acetigena A minerals-salts liquid medium containing potassium phosphate, magnesium chloride, ammonium chloride, potassium chloride, sodium chloride, trace elements, yeast extract, resazurin, sodium carbonate and sodium bicarbonate, ethanol, vitamin solution, and sodium sulfide. Prepared under an atmosphere of nitrogen. Used to support the growth of Natroniella. DSZM Medium 784.1 A minerals-salts liquid medium similar to DSMZ Medium 784, except that ethanol is omitted and sodium thiosulfate is added. DSM 24923 = Natranaerobaculum magadiense Z1001 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium784.pdf 784. NATRONIELLA MEDIUM KH2PO4 0.2 g MgCl2 x 6 H2O 0.1 g NH4Cl 1.0 g KCl 0.2 g NaCl 16.0 g Trace element solution SL-11 (see below) 1.0 ml Yeast extract 0.2 g Resazurin 0.5 mg Na2CO3 68.0 g NaHCO3 38.0 g Ethanol 5.0 ml Vitamin solution (see medium 141) 10.0 ml Na2S x 9 H2O 1.0 g Distilled water 1000.0 ml Trace element solution SL-11: Na2-EDTA 5.2 g FeCl2 x 4 H2O 1.5 g ZnCl2 70.0 mg MnCl2 x 4 H2O 100.0 mg H3BO3 6.0 mg CoCl2 x 6 H2O 190.0 mg CuCl2 x 2 H2O 2.0 mg NiCl2 x 6 H2O 24.0 mg Na2Mo4 x 2 H2O 36.0 mg Distilled water 1000.0 ml Adjust pH of solution to 6.0. Dissolve ingredients except carbonates, ethanol, vitamins and sulfide. Bring medium to the boil, then cool to room temperature while flushing with 100% N2 gas. Add and dissolve carbonates and sulfide while gassing the head space only. Dispense and autoclave under N2 gas atmosphere. Before use add ethanol and vitamins from sterile anoxic stock solution prepared under N2. Vitamins are sterilized by filtration. Adjust pH of the completed medium to 9.5 – 10.0. For DSM 24923 omit ethanol and add to the autoclaved medium 2.0 g/l Na2S2O3 x 5 H2O from a sterile anoxic stock solution. © 2013 DSMZ GmbH - All rights reserved DSMZ Medium 784.1 -< for DSM 24923 trace elements solution SL-11 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium784.pdf 784. NATRONIELLA MEDIUM KH2PO4 0.2 g MgCl2 x 6 H2O 0.1 g NH4Cl 1.0 g KCl 0.2 g NaCl 16.0 g Trace element solution SL-11 (see below) 1.0 ml Yeast extract 0.2 g Resazurin 0.5 mg Na2CO3 68.0 g NaHCO3 38.0 g Ethanol 5.0 ml Vitamin solution (see medium 141) 10.0 ml Na2S x 9 H2O 1.0 g Distilled water 1000.0 ml Trace element solution SL-11: Na2-EDTA 5.2 g FeCl2 x 4 H2O 1.5 g ZnCl2 70.0 mg MnCl2 x 4 H2O 100.0 mg H3BO3 6.0 mg CoCl2 x 6 H2O 190.0 mg CuCl2 x 2 H2O 2.0 mg NiCl2 x 6 H2O 24.0 mg Na2Mo4 x 2 H2O 36.0 mg Distilled water 1000.0 ml Adjust pH of solution to 6.0. Dissolve ingredients except carbonates, ethanol, vitamins and sulfide. Bring medium to the boil, then cool to room temperature while flushing with 100% N2 gas. Add and dissolve carbonates and sulfide while gassing the head space only. Dispense and autoclave under N2 gas atmosphere. Before use add ethanol and vitamins from sterile anoxic stock solution prepared under N2. Vitamins are sterilized by filtration. Adjust pH of the completed medium to 9.5 – 10.0. For DSM 24923 omit ethanol and add to the autoclaved medium 2.0 g/l Na2S2O3 x 5 H2O from a sterile anoxic stock solution. © 2013 DSMZ GmbH - All rights reserved A trace elements solution containing disodium EDTA, ferrous chloride, zinc chloride, manganese chloride, boric acid, cobalt chloride, copper chloride, nickel chloride, and sodium molybdate. washed sheep red blood cells From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Washed, Sheep Cell Suspensions are stabilized preparations of concentrated erythrocytes. A working solution is prepared by diluting the concentrated suspension. It is not recommended that the suspension be washed. Blood medium ingredient comprised of red blood cells from a sheep (Ovis aries) that have been washed. Used to support the growth of microorganisms which need washed red blood cells to grow. Carrine Blank DSMZ Medium 385 A minerals-salts, liquid medium containing sodium sulfate, potassium phosphate, ammonium chloride, magnesium chloride, potassium chloride, calcium chloride, resazurin, trace element solution, sodium bicarbonate, selenite-tungstate solution, sodium benzoate, pyrocatechol (i.e. catechol), hydrochloric acid, vitamin solution, and sodium sulfide. Prepared under an atmosphere of nitrogen and carbon dioxide. Used to support the growth of Desulfobacterium catecholicum. Desulfobacterium catecholicum medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium385.pdf 385. DESULFOBACTERIUM CATECHOLICUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 100.00 ml Solution D: Selenite-tungstate solution (see below) 1.00 ml Solution E: Na-benzoate 0.40 g Distilled water 10.00 ml Solution F: Pyrocatechol 0.06 g 1 N HCl 0.06 ml Distilled water 10.00 ml Solution G: Vitamin solution (see medium 141) 10.00 ml Solution H: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and H are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions E, F and G are prepared under N2 gas and sterilized by filtration. The pyrocatechol stock solution has to be prepared always freshly prior to use. Solution B to H are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 6.9 - 7.1. Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 10 - 20% inoculum. Selenite-tungstate solution: NaOH 0.5 g Na2SeO3 x 5 H2O 3.0 mg Na2WO4 x 2 H2O 4.0 mg Distilled water 1000.0 ml © 2014 DSMZ GmbH - All rights reserved DSM strains: selenite-tungate solution A trace element solution containing sodium hydroxide, sodium selenite, and sodium tungstate. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium385.pdf 385. DESULFOBACTERIUM CATECHOLICUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 100.00 ml Solution D: Selenite-tungstate solution (see below) 1.00 ml Solution E: Na-benzoate 0.40 g Distilled water 10.00 ml Solution F: Pyrocatechol 0.06 g 1 N HCl 0.06 ml Distilled water 10.00 ml Solution G: Vitamin solution (see medium 141) 10.00 ml Solution H: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and H are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions E, F and G are prepared under N2 gas and sterilized by filtration. The pyrocatechol stock solution has to be prepared always freshly prior to use. Solution B to H are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 6.9 - 7.1. Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 10 - 20% inoculum. Selenite-tungstate solution: NaOH 0.5 g Na2SeO3 x 5 H2O 3.0 mg Na2WO4 x 2 H2O 4.0 mg Distilled water 1000.0 ml © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 141 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141.pdf 141. METHANOGENIUM MEDIUM (H2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see below) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Vitamin solution (see below) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. If the medium is being used without gas mixture overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.18 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.18 g CuSO4 x 5 H2O 0.01 g KAl(SO4)2 x 12 H2O 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.03 g Na2SeO3 x 5 H2O 0.30 mg Na2WO4 x 2 H2O 0.40 mg Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml For DSM 1498, DSM 15558, and DSM 22353 adjust pH to 6.5. For DSM 2373 increase the amount of trypticase to 6.00 g/l. For DSM 4254 add a filter-sterilized, anoxic solution of L-histidine to a final concentration of 0.08 g/l. For DSM 7268, DSM 7466, and DSM 15558 use only one atmosphere overpressure of 80% H2 and 20% CO2. For DSM 15219, DSM 16458, DSM 18860, and DSM 21220 adjust pH to 7.5. For DSM 15558 and DSM 16458 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. Use only one atmosphere overpressure of 80% H2 and 20% CO2. © 2014 DSMZ GmbH - All rights reserved A minerals-salts, liquid medium containind potassium chloride, magnesium chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, sodium acetate, yeast extract, trypicase peptone, resazurin, sodium bicarbonate, vitamin solution, L-cysteine, and sodium sulfide. Prepared under an atmosphere of hydrogen, carbon dioxide, and nitrogen. Used to support the growth of Methanogenium. DSM strains: Methanogenium medium Methanogenium medium Carrine Blank trace elements solution for DSMZ Medium 141 A trace elements solution contining nitrilotriacetic acid, magnesium sulfate, manganese sulfate, sodium chloride, ferrous sulfate, cobalt sulfate, calcium chloride, zinc sulfate, copper sulfate, potassium aluminum sulfate, boric acid, sodium molybdate, nickel chloride, sodium selenite, and sodium tungstate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141.pdf 141. METHANOGENIUM MEDIUM (H2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see below) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Vitamin solution (see below) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. If the medium is being used without gas mixture overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.18 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.18 g CuSO4 x 5 H2O 0.01 g KAl(SO4)2 x 12 H2O 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.03 g Na2SeO3 x 5 H2O 0.30 mg Na2WO4 x 2 H2O 0.40 mg Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml For DSM 1498, DSM 15558, and DSM 22353 adjust pH to 6.5. For DSM 2373 increase the amount of trypticase to 6.00 g/l. For DSM 4254 add a filter-sterilized, anoxic solution of L-histidine to a final concentration of 0.08 g/l. For DSM 7268, DSM 7466, and DSM 15558 use only one atmosphere overpressure of 80% H2 and 20% CO2. For DSM 15219, DSM 16458, DSM 18860, and DSM 21220 adjust pH to 7.5. For DSM 15558 and DSM 16458 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. Use only one atmosphere overpressure of 80% H2 and 20% CO2. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 141.1 A minerals-salts liquid medium similar to DSMZ Medium 141, except that the pH has been lowered to 6.5. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141.pdf 141. METHANOGENIUM MEDIUM (H2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see below) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Vitamin solution (see below) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. If the medium is being used without gas mixture overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.18 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.18 g CuSO4 x 5 H2O 0.01 g KAl(SO4)2 x 12 H2O 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.03 g Na2SeO3 x 5 H2O 0.30 mg Na2WO4 x 2 H2O 0.40 mg Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml For DSM 1498, DSM 15558, and DSM 22353 adjust pH to 6.5. For DSM 2373 increase the amount of trypticase to 6.00 g/l. For DSM 4254 add a filter-sterilized, anoxic solution of L-histidine to a final concentration of 0.08 g/l. For DSM 7268, DSM 7466, and DSM 15558 use only one atmosphere overpressure of 80% H2 and 20% CO2. For DSM 15219, DSM 16458, DSM 18860, and DSM 21220 adjust pH to 7.5. For DSM 15558 and DSM 16458 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. Use only one atmosphere overpressure of 80% H2 and 20% CO2. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 141.1 -< for DSM 1498, DSM 15558, and DSM 22353 DSM 1498 is Methanoculleus marisnigri DSM 15558 is Methanogenium marinum DSM 22353 is Methanobacterium petrolearium Carrine Blank DSMZ Medium 141.2 DSM 2373 is Methanoculleus thermophilus DSM 2373 A minerals-salts liquid medium similar to DSMZ Medium 141, except that the concentration of trypicase peptone is increased. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141.pdf 141. METHANOGENIUM MEDIUM (H2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see below) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Vitamin solution (see below) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. If the medium is being used without gas mixture overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.18 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.18 g CuSO4 x 5 H2O 0.01 g KAl(SO4)2 x 12 H2O 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.03 g Na2SeO3 x 5 H2O 0.30 mg Na2WO4 x 2 H2O 0.40 mg Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml For DSM 1498, DSM 15558, and DSM 22353 adjust pH to 6.5. For DSM 2373 increase the amount of trypticase to 6.00 g/l. For DSM 4254 add a filter-sterilized, anoxic solution of L-histidine to a final concentration of 0.08 g/l. For DSM 7268, DSM 7466, and DSM 15558 use only one atmosphere overpressure of 80% H2 and 20% CO2. For DSM 15219, DSM 16458, DSM 18860, and DSM 21220 adjust pH to 7.5. For DSM 15558 and DSM 16458 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. Use only one atmosphere overpressure of 80% H2 and 20% CO2. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 141.2 -< for DSM 2373 DSMZ Medium 141.3 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141.pdf 141. METHANOGENIUM MEDIUM (H2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see below) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Vitamin solution (see below) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. If the medium is being used without gas mixture overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.18 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.18 g CuSO4 x 5 H2O 0.01 g KAl(SO4)2 x 12 H2O 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.03 g Na2SeO3 x 5 H2O 0.30 mg Na2WO4 x 2 H2O 0.40 mg Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml For DSM 1498, DSM 15558, and DSM 22353 adjust pH to 6.5. For DSM 2373 increase the amount of trypticase to 6.00 g/l. For DSM 4254 add a filter-sterilized, anoxic solution of L-histidine to a final concentration of 0.08 g/l. For DSM 7268, DSM 7466, and DSM 15558 use only one atmosphere overpressure of 80% H2 and 20% CO2. For DSM 15219, DSM 16458, DSM 18860, and DSM 21220 adjust pH to 7.5. For DSM 15558 and DSM 16458 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. Use only one atmosphere overpressure of 80% H2 and 20% CO2. © 2014 DSMZ GmbH - All rights reserved A minerals-salts liquid medium similar to DSMZ Medium 141, except that L-histidine has been added. Carrine Blank DSMZ Medium 141.3 -< for DSM 4254 DSM 4254 is Methanococcus voltae DSMZ Medium 141.4 DSM 7268 is Methanothermobacter thermoflexus DSM 7466 is Methanothermobacter defluvii DSM 15558 is Methanogenium marinum Carrine Blank DSMZ Medium 141.4 -< for DSM 7268, DSM 7466, and DSM 15558 A minerals-salts liquid medium similar to DSMZ Medium 141, except that a decreased pressure of H2 and CO2 is used. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141.pdf 141. METHANOGENIUM MEDIUM (H2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see below) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Vitamin solution (see below) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. If the medium is being used without gas mixture overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.18 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.18 g CuSO4 x 5 H2O 0.01 g KAl(SO4)2 x 12 H2O 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.03 g Na2SeO3 x 5 H2O 0.30 mg Na2WO4 x 2 H2O 0.40 mg Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml For DSM 1498, DSM 15558, and DSM 22353 adjust pH to 6.5. For DSM 2373 increase the amount of trypticase to 6.00 g/l. For DSM 4254 add a filter-sterilized, anoxic solution of L-histidine to a final concentration of 0.08 g/l. For DSM 7268, DSM 7466, and DSM 15558 use only one atmosphere overpressure of 80% H2 and 20% CO2. For DSM 15219, DSM 16458, DSM 18860, and DSM 21220 adjust pH to 7.5. For DSM 15558 and DSM 16458 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. Use only one atmosphere overpressure of 80% H2 and 20% CO2. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 141.5 Carrine Blank A minerals-salts liquid medium similar to DSMZ Medium 141, except that the pH has been increased to 7.5. DSM 15219 is Methanobacterium aarhusense DSM 16458 is Methanogenium frigidum DSM 18860 is Methanoculleus receptaculi DSM 21220 is not in www.dsmz. de, TaxBrowser, or StrainInfo. DSMZ Medium 141.5 -< for DSM 15219, DSM 16458, DSM 18860, and DSM 21220 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141.pdf 141. METHANOGENIUM MEDIUM (H2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see below) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Vitamin solution (see below) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. If the medium is being used without gas mixture overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.18 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.18 g CuSO4 x 5 H2O 0.01 g KAl(SO4)2 x 12 H2O 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.03 g Na2SeO3 x 5 H2O 0.30 mg Na2WO4 x 2 H2O 0.40 mg Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml For DSM 1498, DSM 15558, and DSM 22353 adjust pH to 6.5. For DSM 2373 increase the amount of trypticase to 6.00 g/l. For DSM 4254 add a filter-sterilized, anoxic solution of L-histidine to a final concentration of 0.08 g/l. For DSM 7268, DSM 7466, and DSM 15558 use only one atmosphere overpressure of 80% H2 and 20% CO2. For DSM 15219, DSM 16458, DSM 18860, and DSM 21220 adjust pH to 7.5. For DSM 15558 and DSM 16458 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. Use only one atmosphere overpressure of 80% H2 and 20% CO2. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 141.6 A minerals-salts liquid medium similar to DSMZ Medium 141, except that coenzyme M is added and a decreased pressure of H2 and CO2 is used. DSM 15558 is Methanogenium marinum DSM 16458 is Methanogenium frigidum Ace-2 Carrine Blank DSMZ Medium 141.6 -< for DSM 15558 and DSM 16458 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141.pdf 141. METHANOGENIUM MEDIUM (H2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see below) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Vitamin solution (see below) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. If the medium is being used without gas mixture overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.18 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.18 g CuSO4 x 5 H2O 0.01 g KAl(SO4)2 x 12 H2O 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.03 g Na2SeO3 x 5 H2O 0.30 mg Na2WO4 x 2 H2O 0.40 mg Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml For DSM 1498, DSM 15558, and DSM 22353 adjust pH to 6.5. For DSM 2373 increase the amount of trypticase to 6.00 g/l. For DSM 4254 add a filter-sterilized, anoxic solution of L-histidine to a final concentration of 0.08 g/l. For DSM 7268, DSM 7466, and DSM 15558 use only one atmosphere overpressure of 80% H2 and 20% CO2. For DSM 15219, DSM 16458, DSM 18860, and DSM 21220 adjust pH to 7.5. For DSM 15558 and DSM 16458 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. Use only one atmosphere overpressure of 80% H2 and 20% CO2. © 2014 DSMZ GmbH - All rights reserved vitamin solution for DSMZ Medium 141 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141.pdf 141. METHANOGENIUM MEDIUM (H2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see below) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Vitamin solution (see below) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. If the medium is being used without gas mixture overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.18 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.18 g CuSO4 x 5 H2O 0.01 g KAl(SO4)2 x 12 H2O 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.03 g Na2SeO3 x 5 H2O 0.30 mg Na2WO4 x 2 H2O 0.40 mg Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml For DSM 1498, DSM 15558, and DSM 22353 adjust pH to 6.5. For DSM 2373 increase the amount of trypticase to 6.00 g/l. For DSM 4254 add a filter-sterilized, anoxic solution of L-histidine to a final concentration of 0.08 g/l. For DSM 7268, DSM 7466, and DSM 15558 use only one atmosphere overpressure of 80% H2 and 20% CO2. For DSM 15219, DSM 16458, DSM 18860, and DSM 21220 adjust pH to 7.5. For DSM 15558 and DSM 16458 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. Use only one atmosphere overpressure of 80% H2 and 20% CO2. © 2014 DSMZ GmbH - All rights reserved A vitamin solution containing biotin, folic acid, pyridoxine hydrochloride, thiamine, riboflavin, nicotinic acid, calcium pantothenate, vitamin B12 (cobalamin), and lipoic acid. DSMZ Medium 1005 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1005.pdf 1005. THERMODESULFOBIUM MEDIUM K2HPO4 0.78 g KH2PO4 0.75 g Na3EDTA 0.04 g FeSO4 x 7 H2O 0.01 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.03 g NaCl 0.20 g NH4Cl 0.50 g Na2SO4 2.80 g Na-acetate 0.15 g Vitamin solution (see medium 141) 10.00 ml Trace element solution SL-9 (see medium 318) 10.00 ml L-Cysteine-HCl x H2O 0.25 g Distilled water 1000.00 ml Dissolve ingredients (except cysteine), boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere and adjust pH to 5.5 with 10N H2SO4. Dispense under same gas atmosphere in suitable culture vessels (e.g. 20 ml of the medium in 50 ml serum bottles) and autoclave. Prior to inoculation add cysteine from a sterile, anoxic stock solution prepared under N2. Adjust pH to 5.5-6.0, if necessary. After inoculation pressurize vials to 0.5 bar overpressure with 80% H2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved Thermodesulfobium medium A minerals-salts, liquid medium containing potassium phosphate, trisodium EDTA, ferrous sulfate, magnesium sulfate, calcium chloride, sodium chloride, ammonium chloride, sodium sulfate, sodium acetate, vitamin solution, trace elements, and L-cysteine hydrochloride. Prepared under an atmosphere of hydrogen, carbon dioxide, and nitrogen. Used to support the growth of Thermodesulfobium. Carrine Blank DSM strains: DSMZ Medium 318 A minerals-salts, liquid medium comprised of potassium phosphate, sodium chloride, magnesium chloride, calcium chloride, trace elements, ammonium chloride, yeast extract, trypticase (trypticase peptone), resazurin, potassium bicarbonate, vitamin solution, methanol, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of nitrogen and carbon dioxide. Used to support the growth of Methanosarcina. Methanosarcina medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium318.pdf 318. METHANOSARCINA (BCYT) MEDIUM KH2PO4 0.30 g NaCl 0.60 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.08 g Trace element solution (see below) 10.00 ml NH4Cl 1.00 g Yeast extract 0.50 g Trypticase (BBL) 0.50 g Resazurin 0.50 mg KHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml Methanol 5.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, methanol, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic tubes under the same gas atmosphere and autoclave. Add vitamins (sterilized by filtration), methanol, cysteine and sulfide from sterile anoxic stock solutions prepared under N2 and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 6.8. Trace elements: Nitrilotriacetic acid (NTA) 12.800 g FeCl3 x 6 H2O 1.350 g MnCl2 x 4 H2O 0.100 g CoCl2 x 6 H2O 0.024 g CaCl2 x 2 H2O 0.100 g ZnCl2 0.100 g CuCl2 x 2 H2O 0.025 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.024 g NaCl 1.000 g NiCl2 x 6 H2O 0.120 g Na2SeO3 x 5 H2O 0.026 g Distilled water 1000.000 ml First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. © 2014 DSMZ GmbH - All rights reserved BCYT medium BYCT DSM strains: Carrine Blank trace elements solution SL-9 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium318.pdf 318. METHANOSARCINA (BCYT) MEDIUM KH2PO4 0.30 g NaCl 0.60 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.08 g Trace element solution (see below) 10.00 ml NH4Cl 1.00 g Yeast extract 0.50 g Trypticase (BBL) 0.50 g Resazurin 0.50 mg KHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml Methanol 5.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, methanol, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic tubes under the same gas atmosphere and autoclave. Add vitamins (sterilized by filtration), methanol, cysteine and sulfide from sterile anoxic stock solutions prepared under N2 and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 6.8. Trace elements: Nitrilotriacetic acid (NTA) 12.800 g FeCl3 x 6 H2O 1.350 g MnCl2 x 4 H2O 0.100 g CoCl2 x 6 H2O 0.024 g CaCl2 x 2 H2O 0.100 g ZnCl2 0.100 g CuCl2 x 2 H2O 0.025 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.024 g NaCl 1.000 g NiCl2 x 6 H2O 0.120 g Na2SeO3 x 5 H2O 0.026 g Distilled water 1000.000 ml First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. © 2014 DSMZ GmbH - All rights reserved Carrine Blank A trace elements solution containing nitrilotriacetic acid, ferric trichloride, manganese chloride, cobalt chloride, calcium chloride, zinc chloride, copper chloride, boric acid, sodium molybdate, sodium chloride, nickel chloride, and sodium selenite. DSMZ Medium 1027 An organic rich, solid medium containing yeast extract, soluble starch, and agar. Carrine Blank yeast starch agar DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1027.pdf 1027. YEAST STARCH AGAR (A) yeast extract 2.0 g soluble starch 10.0 g agar 15.0 g distilled water 1.0 l adjust pH to 7.3 © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 1026 An organic-rich, solid medium containing xylan, yeast extract, and agar. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1026.pdf 1026. XED-AGAR Xylan 7.0 g Yeast extract 3.0 g Agar 18.0 g Distilled water 1000.0 ml pH 7.0 © 2007 DSMZ GmbH - All rights reserved XED-agar DSM strains: DSMZ Medium 1025 Carrine Blank DSM strains: An organic-rich, liquid culture medium containing sea salts, ammonium chloride, peptone, yeast extract, riboflavin, and glucose. Used for the cultivation of Maricaulis. Maricaulis medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1025.pdf 1025. MARICAULIS MEDIUM Sigma Sea salts 30.0 g NH4Cl 0.5 g Distilled water 1000.0 ml Autoclave and cool the medium. Add from sterile stock solutions, 20 ml 50xPYE and 5 ml 0.2 mg/ml riboflavin (filter sterilised), and 2 ml 50% glucose per liter of medium. 50xPYE: Peptone 100.0 g Yeast extract 50.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved sea salt marine salt Carrine Blank An undefined inorganic chemical mixture comprised of a mixture of salts derived from the evaporation of seawater. undefined inorganic chemical mixture Carrine Blank Inorganic compounds or mixtures of inorganic compounds added to culture media to support growth or metabolism of a microorganism. Because the exact composition of the mixture is unknown, it is referred to as "undefined". DSMZ Medium 1024 Nitratiruptor medium A minerals-salts, liquid medium comprised of sodium nitrate, sodium bicarbonate, sodium thiosulfate, elemental sulfur, vitamin solution, and synthetic seawater. Prepared under an atmosphere of dihydrogen and carbon dioxide. Nitratiruptor and Nitratifractor medium Carrine Blank Nitratifractor medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1024.pdf 1024. NITRATIRUPTOR AND NITRATIFRACTOR MEDIUM NaNO3 1.000 g NaHCO3 1.000 g Na2S2O3 x 5 H20 1.000 g Sulfur,powdered 3.000 g Trace vitamin solution (see medium 141) 10.000 ml DMJ synthetic seawater (see medium 997) 1000.000 ml Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding the vitamins and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, the NaHCO3 and vitamin solution. Increase the 80% H2 + 20% CO2 gas phase pressure to 300 kPa. The final pH is 7.0. © 2009 DSMZ GmbH - All rights reserved DSM strains: defined organic solution Carrine Blank Defined organic chemical mixture which is a solution of organic chemicals in water. trace mineral solution for DSMZ Medium 997 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium997.pdf 997. HNW MEDIUM NaNO3 1.000 g NaHCO3 1.000 g Na2WO4 x 2 H2O 0.100 mg Na2S 0.500 g Trace vitamin solution (see medium 141) 10.000 ml DMJ synthetic seawater (see below) 1000.000 ml To prepare the medium, all compounds of DMJ seawater were dissolved in 1 liter of distilled deionized water, and the pH was adjusted to around 7.0 with NaOH at room temperature prior to autoclaving. After autoclaving, filter-sterilized NaNO3 solution (100 g/l), NaHCO3 solution (100 g/l), Na2S solution (100 g/l); pH 7.5) and trace vitamin solution were added. Then the tubes were tightly sealed with butyl rubber stoppers under a gas phase of 80% H2 + 20% CO2 (300 kPa). Synthetic seawater: NaCl 30.000 g K2HPO4 0.140 g CaCl2 x 2 H2O 0.140 g NH4Cl 0.250 g MgSO4 x 7 H2O 3.400 g MgCl2 x 6 H2O 4.180 g KCl 0.330 g NiCl2 x 6 H2O 0.500 mg Na2SeO3 x 5 H2O 0.500 mg Fe(NH4)2(SO4)2 x 6 H2O 0.010 g Trace mineral solution (see below) 10.000 ml Trace mineral solution: C6H9NO6 1.500 g MgSO4 x 7 H2O 3.000 g MnSO4 x 2 H2O 0.500 g NaCl 1.000 g FeSO4 x 7 H2O 0.100 g CoSO4 x 7 H2O 0.180 g CaCl2 x 2 H2O 0.100 g ZnSO4 x 7 H2O 0.180 g CuSO4 x 5 H2O 0.010 g KAl(SO4)2 x 12 H2O 0.020 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.010 g NiCl2 x 6 H2O 0.025 g Na2SeO3 x 5 H2O 0.300 mg © 2007 DSMZ GmbH - All rights reserved A trace elements solution containing C6H9NO6 (nitrilotriacetic acid), magnesium sulfate, manganese sulfate, sodium chloride, ferrous sulfate, cobalt sulfate, calcium chloride, zinc sulfate, copper sulfate, potassium aluminum sulfate, boric acid, sodium molybdate, nickel chloride, and sodium selenite. Carrine Blank DMJ synthetic seawater solution for DSMZ Medium 997 An inorganic salts solution comprising a synthetic seawater. Comprised of sodium chloride, potassium phosphate, calcium chloride, ammonium chloride, magnesium sulfate, magnesium chloride, potassium chloride, nickel chloride, sodium selenite, ferrous ammonium sulfate, and trace minerals. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium997.pdf 997. HNW MEDIUM NaNO3 1.000 g NaHCO3 1.000 g Na2WO4 x 2 H2O 0.100 mg Na2S 0.500 g Trace vitamin solution (see medium 141) 10.000 ml DMJ synthetic seawater (see below) 1000.000 ml To prepare the medium, all compounds of DMJ seawater were dissolved in 1 liter of distilled deionized water, and the pH was adjusted to around 7.0 with NaOH at room temperature prior to autoclaving. After autoclaving, filter-sterilized NaNO3 solution (100 g/l), NaHCO3 solution (100 g/l), Na2S solution (100 g/l); pH 7.5) and trace vitamin solution were added. Then the tubes were tightly sealed with butyl rubber stoppers under a gas phase of 80% H2 + 20% CO2 (300 kPa). Synthetic seawater: NaCl 30.000 g K2HPO4 0.140 g CaCl2 x 2 H2O 0.140 g NH4Cl 0.250 g MgSO4 x 7 H2O 3.400 g MgCl2 x 6 H2O 4.180 g KCl 0.330 g NiCl2 x 6 H2O 0.500 mg Na2SeO3 x 5 H2O 0.500 mg Fe(NH4)2(SO4)2 x 6 H2O 0.010 g Trace mineral solution (see below) 10.000 ml Trace mineral solution: C6H9NO6 1.500 g MgSO4 x 7 H2O 3.000 g MnSO4 x 2 H2O 0.500 g NaCl 1.000 g FeSO4 x 7 H2O 0.100 g CoSO4 x 7 H2O 0.180 g CaCl2 x 2 H2O 0.100 g ZnSO4 x 7 H2O 0.180 g CuSO4 x 5 H2O 0.010 g KAl(SO4)2 x 12 H2O 0.020 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.010 g NiCl2 x 6 H2O 0.025 g Na2SeO3 x 5 H2O 0.300 mg © 2007 DSMZ GmbH - All rights reserved DMJ synthetic seawater Carrine Blank DSMZ Medium 997 DSM strains: 15103 Persephonella hydrogeniphila 16510 Hydrogenivirga caldilitoris A minerals-salts, liuid medium containing sodium nitrate, sodium bicarbonate, sodium tungstate, sodium sulfide, vitamin solution, and synthetic seawater. Prepared under an atmosphere of dihydrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium997.pdf 997. HNW MEDIUM NaNO3 1.000 g NaHCO3 1.000 g Na2WO4 x 2 H2O 0.100 mg Na2S 0.500 g Trace vitamin solution (see medium 141) 10.000 ml DMJ synthetic seawater (see below) 1000.000 ml To prepare the medium, all compounds of DMJ seawater were dissolved in 1 liter of distilled deionized water, and the pH was adjusted to around 7.0 with NaOH at room temperature prior to autoclaving. After autoclaving, filter-sterilized NaNO3 solution (100 g/l), NaHCO3 solution (100 g/l), Na2S solution (100 g/l); pH 7.5) and trace vitamin solution were added. Then the tubes were tightly sealed with butyl rubber stoppers under a gas phase of 80% H2 + 20% CO2 (300 kPa). Synthetic seawater: NaCl 30.000 g K2HPO4 0.140 g CaCl2 x 2 H2O 0.140 g NH4Cl 0.250 g MgSO4 x 7 H2O 3.400 g MgCl2 x 6 H2O 4.180 g KCl 0.330 g NiCl2 x 6 H2O 0.500 mg Na2SeO3 x 5 H2O 0.500 mg Fe(NH4)2(SO4)2 x 6 H2O 0.010 g Trace mineral solution (see below) 10.000 ml Trace mineral solution: C6H9NO6 1.500 g MgSO4 x 7 H2O 3.000 g MnSO4 x 2 H2O 0.500 g NaCl 1.000 g FeSO4 x 7 H2O 0.100 g CoSO4 x 7 H2O 0.180 g CaCl2 x 2 H2O 0.100 g ZnSO4 x 7 H2O 0.180 g CuSO4 x 5 H2O 0.010 g KAl(SO4)2 x 12 H2O 0.020 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.010 g NiCl2 x 6 H2O 0.025 g Na2SeO3 x 5 H2O 0.300 mg © 2007 DSMZ GmbH - All rights reserved HNW medium Carrine Blank inorganic salts solution A solution of inorganic (mostly non-metal) salts. Used to support the growth of microorganisms. Carrine Blank DSMZ Medium 1023 DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1023.pdf 1023. MANNING MEDIUM (NH4)2SO4 6.00 g KCl 0.20 g K2HPO4 0.20 g MgSO4 x 7 H2O 1.00 g Ca(NO3)2 0.02 g Distilled water 1000.00 ml Solution A: FeSO4 x 7 H2O 33.40 g/l Solution B: Yeast extract 0.20 g/l Solution A is separately autoclaved and added to the medium. The medium is supplemented with Solution B (yeast extract). Medium pH adjusted by 0.1 N H2SO4 to pH 2.5 - 2.7. © 2007 DSMZ GmbH - All rights reserved Manning medium A minerals-salts liquid medium that contains ammonium sulfate, potassium chloride, potassium phosphate, calcium nitrate, ferrous sulfate, and yeast extract. DSMZ Medium 1021 'has redox' some 'oxidizing redox' Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1021.pdf 1021. SODALIS GLOSSINIDIUS MEDIUM Solution A: Yeast extract 6.20 g Lactalbumin hydrolysate or Casein hydrolysate 8.10 g D-Glucose 5.00 g KCl 0.25 g MgCl2 x 6 H2O 0.12 g CaCl2 x 2 H2O 0.25 g NaCl 8.70 g NaHCO3 0.15 g Agar 17.00 g Distilled water 900.00 ml Adjust pH to 8.0. Solution B: NaH2PO4 x 2 H2O 0.28 g Distilled water 100.00 ml Sterilize solution A and B separately at 121˚C for 20 min. Cool to 50˚C, then combine. To 4 parts of the above, add 1 part sterile foetal or new borne calf serum. Liquid medium should be filter sterilized instead of autoclaved. © 2007 DSMZ GmbH - All rights reserved An organic-rich, solid medium containing yeast extract, lactalbumin hydrolysate or casein hydrolysate, D-glucose, potassium chloride, magnesium chloride, calcium chloride, sodium chloride, sodium bicarbonate, agar, sodium phosphate, and fetal calf serum or calf serum. Used for the cultivation of Sodalis glossinidius. DSM strains: Sodalis glossinidius medium DSMZ Medium 503 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved FWM medium Carrine Blank A minerals-salts, liquid medium containing potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, resazurin, trace elements, vitamin solution, selenite-tungstate solution, sodium bicarbonate, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: 25964 Aminivibrio pyruvatiphilus 19636 Ammonifex thiophilus 11045 Bilophila wadsworthia 11046 Castellaniella denitrificans 5847 Clostridium homopropionicum DSM 5847 11261 Lachnospiraceae bacterium 19gly4 15206 Clostridium tunisiense TJ 6779 Cytophaga xylanolytica 5651 Desulfococcus biacutus 16082 Desulfotomaculum sp. Ox39 11493 Desulfovibrio 11489 Geobacter 11263 Geovibrio thiophilus 21662 Magnetospirillum bellicus 15978 Methanomethylovorans hollandica DSM 15978 27305 Methanomethylovorans uponensis 14424 Opitutus sp. VeGlc2 15970 Parasporobacterium paucivorans DSM 15970 12018 Propionivibrio pelophilus DSM 12018 5849 Propionivibrio 24984 Seleniivibrio woodruffii 24856 Thermoanaerobaculum aquaticum 11262 Peptostreptococcaceae bacterium 19gly3 seven vitamins solution for DSMZ medium 503 A vitamin solution comprised of vitamin B12 (cobalamin), p-aminobenzoic acid (4-aminobenzoic acid), biotin, nicotinic acid, calcium pantothenate, pyridoxine hydrochloride, and thiamine hydrochloride. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved seven vitamins solution DSMZ Medium 503.1 DSMZ Medium 503.1 -< for DSM 5847 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 503, except adds 3-hydroxybutyrate or D-fructose as a substrate. DSM 5847 is Clostridium homopropionicum DSM 5847 Carrine Blank fetal calf serum foetal calf serum A serum medium ingredient, derived from the serum (coagulated blood) from a fetal calf of a slaughtered cow (Bos taurus). Carrine Blank fetal bovine serum Wikipedia: Fetal bovine serum Fetal bovine serum (FBS) or fetal calf serum is the blood fraction remaining after the natural coagulation of blood, followed by centrifugation to remove any remaining red blood cells.[1] Fetal bovine serum comes from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse. Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. This is due to it having a very low level of antibodies and containing more growth factors, allowing for versatility in many different cell culture applications. The globular protein, bovine serum albumin (BSA), is a major component of fetal bovine serum. The rich variety of proteins in fetal bovine serum maintains cultured cells in a medium in which they can survive, grow, and divide. foetal bovine serum DSMZ Medium 1016.1 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1016.pdf 1016. IDIOMARINA MEDIUM Glucose 10.0 g Proteose peptone 5.0 g Yeast extract 3.0 g Malt extract 3.0 g NaCl 30.0-60.0 g Agar (if required) 18.0 g Distilled water 1000.0 ml DSM 16139 and DSM 16140 have been grown in medium containing 45 g/l NaCl. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSM 16139 is Idiomarina fontislapidosi DSM 16140 is Idiomarina ramblicola An organic-rich, solid medium containing glucose, proteose peptone, yeast extract, malt extract, sodium chloride (45 grams per liter), and agar. DSMZ Medium 1016.1 -< for DSM 16139 and DSM 16140 DSMZ Medium 1016 An organic-rich, solid medium containing glucose, proteose peptone, yeast extract, malt extract, sodium chloride (variable amounts, from 30 to 60 grams per liter), and agar. Carrine Blank DSM strains: Idiomarina medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1016.pdf 1016. IDIOMARINA MEDIUM Glucose 10.0 g Proteose peptone 5.0 g Yeast extract 3.0 g Malt extract 3.0 g NaCl 30.0-60.0 g Agar (if required) 18.0 g Distilled water 1000.0 ml DSM 16139 and DSM 16140 have been grown in medium containing 45 g/l NaCl. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 1017 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1017.pdf 1017. YPG MEDIUM Yeast extract 10.0 g/l Peptone (Difco) 10.0 g/l Glucose 70.0 g/l Distilled water 1000.0 ml Adjust the pH to 6.0 with dilute HCl. Solid media may be prepared by adding 15g/l agar. © 2007 DSMZ GmbH - All rights reserved YPG medium DSM strains: An organic-rich, liquid medium containing yeast extract, peptone, and glucose. Carrine Blank DSMZ Medium 1018 DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1018.pdf 1018. HALOFERAX SULFURIFONTIS MEDIUM Yeast extract 5.0 g/l NaCl 150.0 g/l MgCl2 20.0 g/l K2SO4 0.5 g/l CaCl2 0.1 g/l Distilled water 1000.0 ml pH 7.0 The medium may be solidified by adding 20 g/l agar. © 2007 DSMZ GmbH - All rights reserved Haloferax sulfurifontis medium An organic rich, liquid medium containing yeast extract, sodium chloride, magnesium chloride, potassium sulfate, and calcium chloride. Carrine Blank trace elements solution for DSMZ Medium 1019 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1019.pdf 1019. ROUF'S MEDIUM Yeast extract 5.00 g Peptone 5.00 g MgSO4 x 7 H2O 0.20 g CaCl2 x 6 H2O 0.05 g Fe(NH3)citrate 0.015 g MnSO4 x 4 H2O 0.05 g FeCl3 x 4 H2O 0.01 g Vitamin solution (see medium 141) 10.00 ml Trace elements solution (see below) 1.00 ml Distilled water 1000.00 ml pH 7.1 (adjusted with NaOH if needed) The medium may be solifidied with 17 g/l agar. Trace elements solution: Nitrilotriacetic acid 12.80 g CoCl2 x 6 H2O 0.17 g CaCl2 x 2 H2O 0.10 g FeSO4 x 7 H20 0.10 g MnCl2 x 4 H2O 0.10 g NaCl 0.10 g Na2MoO4 x 2 H2O 0.10 g Distilled water 1000.00 ml First dissolve the nitrilotriacetic acid and adjust the pH to 6.5 with KOH, then add the minerals. The final pH is 7.0, adjusted with KOH. © 2007 DSMZ GmbH - All rights reserved A trace elements solution containing nitrilotriacetic acid, cobalt chloride, calcium chloride, ferrous sulfate, manganese chloride, sodium chloride, and sodium molybdate. Carrine Blank DSMZ Medium 1019 An organic-rich, liquid medium containing yeast extract, peptone, magnesium sulfate, calcium chloride, ferric ammonium citrate, manganese sulfate, iron trichloride, vitamin solution, and trace elements. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1019.pdf 1019. ROUF'S MEDIUM Yeast extract 5.00 g Peptone 5.00 g MgSO4 x 7 H2O 0.20 g CaCl2 x 6 H2O 0.05 g Fe(NH3)citrate 0.015 g MnSO4 x 4 H2O 0.05 g FeCl3 x 4 H2O 0.01 g Vitamin solution (see medium 141) 10.00 ml Trace elements solution (see below) 1.00 ml Distilled water 1000.00 ml pH 7.1 (adjusted with NaOH if needed) The medium may be solifidied with 17 g/l agar. Trace elements solution: Nitrilotriacetic acid 12.80 g CoCl2 x 6 H2O 0.17 g CaCl2 x 2 H2O 0.10 g FeSO4 x 7 H20 0.10 g MnCl2 x 4 H2O 0.10 g NaCl 0.10 g Na2MoO4 x 2 H2O 0.10 g Distilled water 1000.00 ml First dissolve the nitrilotriacetic acid and adjust the pH to 6.5 with KOH, then add the minerals. The final pH is 7.0, adjusted with KOH. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: Rouf's medium DSMZ Medium 1020 MBM Medium (modified) DSM strains: modified MBM medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1020.pdf 1020. MBM MEDIUM (modified) NaNO3 0.2 g KH2PO4 0.2 g NH4Cl 0.2 g MgCl2 x 6 H2O 0.4 g KCl 0.2 g CaCl2 x 2 H2O 0.1 g Na2S2O3 x 5 H2O 2.5 g SL-4 trace element solution (see medium 14) 2.0 ml Resazurin 1.0 mg Distilled water 1000.0 ml Dissolve ingredients, except SL-4 trace element solution and sodium thiosulfate, then flush solution with 80% N2 and 20% CO2 gas mixture to make it anoxic. Distribute medium under same gas atmosphere into culture vessels (e.g. 20 ml in 120 ml serum bottles) and autoclave. Filter-sterilize under anoxic conditions the SL-4 trace element solution and a stock solution of sodium thiosulfate. Prior to inoculation, add the trace element solution and sodium thiosulfate to the medium, and adjust pH to 7.0 if necessary. After inoculation, pressurize culture vessels to 0.5 bar overpressure with 100% H2 gas. © 2007 DSMZ GmbH - All rights reserved Carrine Blank A minerals-salts, liquid medium containing sodium nitrate, potassium phosphate, ammonium chloride, mangesium chloride, potassium chloride, calcium chloride, sodium thiosulfate, trace elements, and resazurin. Prepared under an atmosphere of nitrogen, carbon dioxide, and hydrogen. liver digest http://www.oxoid.com/uk/blue/prod_detail/prod_detail.asp?pr=LP0027&c=uk&lang=EN LIVER DIGEST NEUTRALISED Code: LP0027 A biologically standardised papaic digest of liver for use as a source of nutrients in microbiological culture media. The digest is water soluble and compatible with other culture media ingredients and may be sterilised by filtration or autoclaving; thus it is suitable for use as an integral part of many culture media or as a valuable supplement. Being derived from liver this product contains relatively high levels of iron. The profile shows the characteristic even spread of peptides obtained from papaic digests. Carrine Blank Papaic digest of mammalian liver. DSMZ Medium 503.2 Carrine Blank DSM 5849 is Propioniovibrio http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 503, except adds sodium succinate as a substrate. DSMZ Medium 503.2 -< for DSM 5849 DSMZ Medium 503.3 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 503, except adds sodium maleate as a substrate and adjusts the pH down to 6.7-6.8. Carrine Blank DSM 5885 is Propionivibrio dicarboxylicus DSM 5885 DSMZ Medium 503.3 -< for DSM 5885 DSMZ Medium 503.4 Carrine Blank DSMZ Medium 503.4 -< for DSM 6779 DSM 6779 is Cytophaga xylanolytica Similar to DSMZ Medium 503, except that xylan or xylose is added as a substate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 503.5 Carrine Blank DSMZ Medium 503.5 -< for DSM 10092 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSM 10092 is Syntrophobacter pfennigii Similar to DSMZ Medium 503, except that sodium sulfate is added and sodium propionate is added as a substrate. The amount of sodium sulfide is reduced and sodium dithionite is added. DSMZ Medium 503.6 DSMZ Medium 503.6 -< for DSM 11046 Similar to DSMZ Medium 503, except sodium nitrate is added and taurine is added as a substrate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 11046 is Castellaniella denitrificans DSMZ Medium 503.7 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 503, except yeast extract and sodium glycolate are added as substrates. DSMZ Medium 503.7 -< for DSM 11261 Carrine Blank DSM 11261 is Lachnospiraceae bacterium 19gly4 DSMZ Medium 503.8 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 503.8 -< for DSM 11262 Carrine Blank DSM 11262 is Peptostreptococcaceae bacterium 19gly3 Similar to DSMZ Medium 503, except yeast extract and 3-hydroxybutyrate are added as substrates. DSMZ Medium 503.9 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 503.9 -< for DSM 11263 and DSM 11489 Similar to DSMZ Medium 503, except sodium acetate and sodium fumarate are added as substrates. Carrine Blank DSM 11263 is Geovibrio thiophilus DSM 11489 is Geobacter sp. DSMZ Medium 503.10 DSMZ Medium 503.10 -< for DSM 11270 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 503, except traurine is added as a substrate. DSM 11270 is Desulfonispora thiosulfatigenes DSM 11270 DSMZ Medium 503.11 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 503.11 -< for DSM 11480 DSM 11480 is "unclassified bacterium". No sequences associated with this strain. Not in Tax Browser. Similar to DSMZ Medium 503, except D-glucose is added as a substrate. DSMZ Medium 503.12 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSM 12018 is Propionivibrio pelophilus DSM 12018 DSMZ Medium 503.12 -< for DSM 12018 Similar to DSMZ Medium 503, except yeast extract and L-aspartic acid are added as substrates. DSMZ Medium 503.13 Similar to DSMZ Medium 503, except yeast extract and D-fructose are added as substrates. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 503.13 -< for DSM 13305 Carrine Blank DSM 13305 is Propionispora vibrioides DSM 13305 DSMZ Medium 503.14 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 503, except glucose and yeast extract are added as substrates. DSMZ Medium 503.14 -< for DSM 14424 and DSM 28450 DSM 14424 is Opitutus sp. VeGlc2 DSM 28450 is Fusibacter sp. KhalAKB1 DSMZ Medium 503.15 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 503.15 -< for DSM 15206 Similar to DSMZ Medium 503, except yeast extract and trypticase peptone are added as substrates. Carrine Blank DSM 15206 is Clostridium tunisiense TJ DSMZ Medium 503.16 DSM 15978 is Methanomethylovorans hollandica DSM 15978 DSM 27305 is Methanomethylovorans uponensis DSMZ Medium 503.16 -< for DSM 15978 and DSM 27305 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 503, except trimethylamine hydrochloride, methanol, and ferrous sulfate heptahydrate are added as substrates, and the pH is lowered to 6.5-7.0. DSMZ Medium 503.17 DSMZ Medium 503.17 -< for DSM 16082 Similar to DSMZ Medium 503, except sodium sulfate, 2,2,4,4,6,8,8-heptamethylnonane, m-xylene, ferrous sulfate heptahydrate, and sulfuric acid are added. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSM 16082 is Desulfotomaculum sp. Ox39 DSMZ Medium 503.18 Carrine Blank DSMZ Medium 503.18 -< for DSM 21662 DSM 21662 is Magnetospirillum bellicus http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 503, except trace elements solution SL-10 and selenite-tungstate solution are replaced with the trace elements of medium 141, solution C with the vitamins solution of medium 141, and sodium sulfide is omitted. Added is sodium acetate and sodium perchlorate. mineral solution for DSMZ Medium 330 An inorganic salts solution containing potassium phosphate, sodium chloride, ammonium sulfate, calcium chloride, and magnesium sulfate. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium330.pdf 330. RUMEN BACTERIA MEDIUM Mineral solution (see below) 38.00 ml K2HPO4 0.30 g Trypticase peptone (BD BBL) 2.00 g Yeast extract 0.50 g Volatile fatty acid mixture (see below) 3.10 ml Haemin 1.00 mg Glycerol 0.50 g Resazurin 1.00 mg Na2CO3 4.00 g D-Glucose 0.50 g Maltose 0.50 g Cellobiose 0.50 g Starch, soluble 0.50 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 960.00 ml Dissolve ingredients (except carbonate, carbohydrates, cysteine, and sulfide), bring medium to the boil, then cool to room temperature while gassing with 100% CO2 gas. Add the carbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under CO2 gas atmosphere in rubber stoppered tubes (i.e. Hungate-type tubes) and autoclave. Thereafter, add carbohydrates, cysteine and sulfide from sterile stock solutions (prepared anoxically under 100% N2 gas and autoclaved). Adjust pH of final medium to 6.7 - 6.8, if necessary. Mineral solution: KH2PO4 6.00 g NaCl 12.00 g (NH4)2SO4 6.00 g CaCl2 x 2 H2O 1.60 g MgSO4 x 7 H2O 2.50 g Distilled water 1000.00 ml Volatile fatty acid mixture: Acetic acid 4.25 ml Propionic acid 1.50 ml Butyric acid 1.00 ml n-Valeric acid 0.25 ml iso-Butyric acid 0.25 ml DL-2-Methyl butyric acid 0.25 ml iso-Valeric acid 0.25 ml © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 330 Carrine Blank DSM strains: An organic rich, liquid medium containing mineral solution, potassium phosphate, trypticase peptone, yeast extract, volatile fatty acids, hemin, glycerol, resazurin, sodium carbonate, D-glucose, maltose, cellobiose, soluble starch, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of carbon dioxide and nitrogen. Used for the cultivation of rumen bacteria. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium330.pdf 330. RUMEN BACTERIA MEDIUM Mineral solution (see below) 38.00 ml K2HPO4 0.30 g Trypticase peptone (BD BBL) 2.00 g Yeast extract 0.50 g Volatile fatty acid mixture (see below) 3.10 ml Haemin 1.00 mg Glycerol 0.50 g Resazurin 1.00 mg Na2CO3 4.00 g D-Glucose 0.50 g Maltose 0.50 g Cellobiose 0.50 g Starch, soluble 0.50 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 960.00 ml Dissolve ingredients (except carbonate, carbohydrates, cysteine, and sulfide), bring medium to the boil, then cool to room temperature while gassing with 100% CO2 gas. Add the carbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under CO2 gas atmosphere in rubber stoppered tubes (i.e. Hungate-type tubes) and autoclave. Thereafter, add carbohydrates, cysteine and sulfide from sterile stock solutions (prepared anoxically under 100% N2 gas and autoclaved). Adjust pH of final medium to 6.7 - 6.8, if necessary. Mineral solution: KH2PO4 6.00 g NaCl 12.00 g (NH4)2SO4 6.00 g CaCl2 x 2 H2O 1.60 g MgSO4 x 7 H2O 2.50 g Distilled water 1000.00 ml Volatile fatty acid mixture: Acetic acid 4.25 ml Propionic acid 1.50 ml Butyric acid 1.00 ml n-Valeric acid 0.25 ml iso-Butyric acid 0.25 ml DL-2-Methyl butyric acid 0.25 ml iso-Valeric acid 0.25 ml © 2014 DSMZ GmbH - All rights reserved Rumen bacteria medium volatile fatty acid mixture for DSMZ Medium 330 Carrine Blank DSMZ Medium 330: 330. RUMEN BACTERIA MEDIUM Mineral solution (see below) 38.00 ml K2HPO4 0.30 g Trypticase peptone (BD BBL) 2.00 g Yeast extract 0.50 g Volatile fatty acid mixture (see below) 3.10 ml Haemin 1.00 mg Glycerol 0.50 g Resazurin 1.00 mg Na2CO3 4.00 g D-Glucose 0.50 g Maltose 0.50 g Cellobiose 0.50 g Starch, soluble 0.50 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 960.00 ml Dissolve ingredients (except carbonate, carbohydrates, cysteine, and sulfide), bring medium to the boil, then cool to room temperature while gassing with 100% CO2 gas. Add the carbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under CO2 gas atmosphere in rubber stoppered tubes (i.e. Hungate-type tubes) and autoclave. Thereafter, add carbohydrates, cysteine and sulfide from sterile stock solutions (prepared anoxically under 100% N2 gas and autoclaved). Adjust pH of final medium to 6.7 - 6.8, if necessary. Mineral solution: KH2PO4 6.00 g NaCl 12.00 g (NH4)2SO4 6.00 g CaCl2 x 2 H2O 1.60 g MgSO4 x 7 H2O 2.50 g Distilled water 1000.00 ml Volatile fatty acid mixture: Acetic acid 4.25 ml Propionic acid 1.50 ml Butyric acid 1.00 ml n-Valeric acid 0.25 ml iso-Butyric acid 0.25 ml DL-2-Methyl butyric acid 0.25 ml iso-Valeric acid 0.25 ml © 2014 DSMZ GmbH - All rights reserved A defined organic solution containing a mixture the following volatile fatty acids: acetic acid, propionic acid, butyric acid, valeric acid, isobutyric acid, 2-methylbutyric acid, and isovaleric acid. DSMZ Medium 1006 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1006.pdf 1006. ALLISONELLA MEDIUM K2HPO4 292.0 mg KH2PO4 292.0 mg (NH4)2SO4 480.0 mg NaCl 480.0 mg MgSO4 x 7 H2O 100.0 mg CaCl2 x 2 H2O 64.0 mg Trypticase 1.0 g Yeast extract 4.0 g DL-Histidine 7.8 g Resazurin 1.0 mg Na2CO3 4.0 g Volatile fatty acid mixture (see medium 330) 3.1 ml L-Cysteine-HCl x H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except carbonate, fatty acid mixture and cysteine), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate, fatty acid mixture and cysteine and adjust pH to 6.0. Dispense under same gas atmosphere in culture vessels and autoclave. © 2015 DSMZ GmbH - All rights reserved DSM strains: Allisonella medium An organic-rich, liquid medium containing potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, trypticase (trypticase peptone), yeast extract, histidine, resazurin, sodium carbonate, volatile fatty acid mixture, and L-cysteine hydrochloride. Prepared under an atmosphere of carbon dioxide. Carrine Blank DSMZ Medium 1007.4 DSM 24478 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSM 24479 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSM 24480 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSM 24481 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSM 24492 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSM 24493 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSM 24527 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSMZ Medium 1007.4 -< for DSM 24478, 24479, 24480, 24481, 24492, 24493, 24527 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1007.pdf 1007. MINERAL MEDIUM KNO3 250.0 mg KH2PO4 100.0 mg MgSO4 x 7 H2O 50.0 mg CaCl2 x 2 H2O 10.0 mg Trace elements 1.0 ml Distilled water 1000.0 ml Trace elements: EDTA 5.00 g CuCl2 x 5 H2O 0.10 g FeSO4 x 7 H2O 2.00 g ZnSO4 x 7 H2O 0.10 g NiCl2 x 6 H2O 0.02 g CoCl2 x 6 H2O 0.20 g Na2MoO4 0.03 g MnCl2 x 4 H2O 0.03 g Distilled water 1000.00 ml Final pH 5.5-6.0. DSM 15672 may be grown on either 0.5-1.0% methanol or under a gas phase of 20% methane in air. DSM 15673 supplied from the DSMZ has been grown on methanol. DSM 16984 has been grown on 20% methane in air. DSM 24478, 24479, 24480, 24481, 24492, 24493 and 24527 have been grown on 50% methane in air. Strains should be grown with shaking when grown on methane. © 2012 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 1007, except that methane (in higher amounts) is added. Carrine Blank DSMZ Medium 1007.3 DSM 16984 is Methylocystis heyeri DSMZ Medium 1007.3 -< for DSM 16984 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1007.pdf 1007. MINERAL MEDIUM KNO3 250.0 mg KH2PO4 100.0 mg MgSO4 x 7 H2O 50.0 mg CaCl2 x 2 H2O 10.0 mg Trace elements 1.0 ml Distilled water 1000.0 ml Trace elements: EDTA 5.00 g CuCl2 x 5 H2O 0.10 g FeSO4 x 7 H2O 2.00 g ZnSO4 x 7 H2O 0.10 g NiCl2 x 6 H2O 0.02 g CoCl2 x 6 H2O 0.20 g Na2MoO4 0.03 g MnCl2 x 4 H2O 0.03 g Distilled water 1000.00 ml Final pH 5.5-6.0. DSM 15672 may be grown on either 0.5-1.0% methanol or under a gas phase of 20% methane in air. DSM 15673 supplied from the DSMZ has been grown on methanol. DSM 16984 has been grown on 20% methane in air. DSM 24478, 24479, 24480, 24481, 24492, 24493 and 24527 have been grown on 50% methane in air. Strains should be grown with shaking when grown on methane. © 2012 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 1007, except that methane is added. DSMZ Medium 1007.2 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1007.pdf 1007. MINERAL MEDIUM KNO3 250.0 mg KH2PO4 100.0 mg MgSO4 x 7 H2O 50.0 mg CaCl2 x 2 H2O 10.0 mg Trace elements 1.0 ml Distilled water 1000.0 ml Trace elements: EDTA 5.00 g CuCl2 x 5 H2O 0.10 g FeSO4 x 7 H2O 2.00 g ZnSO4 x 7 H2O 0.10 g NiCl2 x 6 H2O 0.02 g CoCl2 x 6 H2O 0.20 g Na2MoO4 0.03 g MnCl2 x 4 H2O 0.03 g Distilled water 1000.00 ml Final pH 5.5-6.0. DSM 15672 may be grown on either 0.5-1.0% methanol or under a gas phase of 20% methane in air. DSM 15673 supplied from the DSMZ has been grown on methanol. DSM 16984 has been grown on 20% methane in air. DSM 24478, 24479, 24480, 24481, 24492, 24493 and 24527 have been grown on 50% methane in air. Strains should be grown with shaking when grown on methane. © 2012 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 1007, except that methanol is added. DSM 15673 is Methylocella tundrae DSMZ Medium 1007.2 -< for DSM 15673 DSMZ Medium 1007.1 DSM 15672 is Alteromonas stellipolaris LMG 21856 Carrine Blank Similar to DSMZ Medium 1007, except that methanol, methane, and air (oxygen) is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1007.pdf 1007. MINERAL MEDIUM KNO3 250.0 mg KH2PO4 100.0 mg MgSO4 x 7 H2O 50.0 mg CaCl2 x 2 H2O 10.0 mg Trace elements 1.0 ml Distilled water 1000.0 ml Trace elements: EDTA 5.00 g CuCl2 x 5 H2O 0.10 g FeSO4 x 7 H2O 2.00 g ZnSO4 x 7 H2O 0.10 g NiCl2 x 6 H2O 0.02 g CoCl2 x 6 H2O 0.20 g Na2MoO4 0.03 g MnCl2 x 4 H2O 0.03 g Distilled water 1000.00 ml Final pH 5.5-6.0. DSM 15672 may be grown on either 0.5-1.0% methanol or under a gas phase of 20% methane in air. DSM 15673 supplied from the DSMZ has been grown on methanol. DSM 16984 has been grown on 20% methane in air. DSM 24478, 24479, 24480, 24481, 24492, 24493 and 24527 have been grown on 50% methane in air. Strains should be grown with shaking when grown on methane. © 2012 DSMZ GmbH - All rights reserved DSMZ Medium 1007.1 -< for DSM 15672 DSMZ Medium 1007 A minerals-salts, liquid medium containing potassium nitrate, potassium phosphate, magnesium sulfate, calcium chloride, and trace elements. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1007.pdf 1007. MINERAL MEDIUM KNO3 250.0 mg KH2PO4 100.0 mg MgSO4 x 7 H2O 50.0 mg CaCl2 x 2 H2O 10.0 mg Trace elements 1.0 ml Distilled water 1000.0 ml Trace elements: EDTA 5.00 g CuCl2 x 5 H2O 0.10 g FeSO4 x 7 H2O 2.00 g ZnSO4 x 7 H2O 0.10 g NiCl2 x 6 H2O 0.02 g CoCl2 x 6 H2O 0.20 g Na2MoO4 0.03 g MnCl2 x 4 H2O 0.03 g Distilled water 1000.00 ml Final pH 5.5-6.0. DSM 15672 may be grown on either 0.5-1.0% methanol or under a gas phase of 20% methane in air. DSM 15673 supplied from the DSMZ has been grown on methanol. DSM 16984 has been grown on 20% methane in air. DSM 24478, 24479, 24480, 24481, 24492, 24493 and 24527 have been grown on 50% methane in air. Strains should be grown with shaking when grown on methane. © 2012 DSMZ GmbH - All rights reserved DSM strains: mineral medium DSMZ Medium 1008 Marine spirochete medium An organic-rich, liquid medium containing trypticase peptone, yeast extract, sodium thioglycolate, resazurin, charcoal-filtered seawater and cellobiose. Prepared under at atmosphere of dinitrogen. Used for the cultivation of marine spirochaetes. Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1008.pdf 1008. MARINE SPIROCHETE MEDIUM Trypticase Peptone (BD 211921) 2.0 g Yeast extract 1.0 g Na-Thioglycolate 1.0 g Resazurin 0.5 mg Charcoal-filtered, natural seawater 800.0 ml Distilled water 200.0 ml Dissolve ingredients (except thioglycolate), boil medium for 3 min., then cool to room temperature under N2 gas atmosphere. Add thioglycolate and adjust pH of medium to 7.5 with 10 N KOH. Dispense under N2 gas atmosphere in culture vessels and autoclave at 121 ˚C for 15 min. Bottled water from Biomaris GmbH can be used instead of filtered seawater. Prepare 10% cellobiose solution (10.0 g in 100 ml distilled water) under nitrogen atmosphere, filter-sterilize and add 0.2 ml to 10 ml autoclaved medium. © 2009 DSMZ GmbH - All rights reserved DSMZ Medium 1009 DSM strains: TCG medium An organic-rich, liquid medium comprised of tryptone, casitone, glucose, and artificial seawater. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1009.pdf 1009. TCG MEDIUM Tryptone 3.0 g Casitone 5.0 g Glucose 4.0 g Seawater (see below) 1000.0 ml Artifical seawater (ASW, eg., Instant Ocean) = 32 g seasalt disolved in 1000 ml distilled water. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 1010.1 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1010.pdf 1010. ARTIFICAL SEAWATER MEDIUM NaCl 26.40 g MgCl2 x 6 H2O 5.70 g MgSO4 x 7 H2O 6.80 g KCl 0.66 g CaCl2 x 2 H2O 1.47 g KBr 0.09 g Selenite-tungstate solution (see medium 385) 1.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg KH2PO4 0.20 g NH4Cl 0.25 g NaHCO3 2.50 g Na-lactate 2.30 g Vitamin solution (see medium 141) 10.00 ml Seven vitamins solution (see medium 503) 1.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except dihydrogenphosphate, ammonium chloride, bicarbonate, lactate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Dispense under same gas atmosphere in culture vessels and autoclave. Add dihydrogenphosphate, ammonium chloride, bicarbonate, lactate, vitamins and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas or 80% N2 and 20% CO2 gas mixture (bicarbonate). The vitamin solutions should be sterilized by filtration. Adjust the final pH of the medium to 7.2 - 7.5. For DSM 15769 replace lactate as substrate with 0.45 g/l 3-phenylpropionate, which is added to the medium after autoclaving from a sterile anoxic stock solution prepared under 100% N2. DSMZ Medium 1010.1 -< for DSM 15769 DSM 15769 is Delta proteobacterium EbS7 Carrine Blank Similar to DSMZ Medium 1010, except that lactate is omitted and 3-phenylpropionate is added. DSMZ Medium 1010 A minerals-salts, liquid medium containing sodium chloride, magnesium chloride, magnesium sulfate, potassium chloride, calcium chloride, potassium bromide, selenite-tungstate solution, trace elements, resazurin, potassium phosphate, ammonium chloride, sodium bicarbonate, sodium lactate, vitamin solution, seven vitamins solution, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1010.pdf 1010. ARTIFICAL SEAWATER MEDIUM NaCl 26.40 g MgCl2 x 6 H2O 5.70 g MgSO4 x 7 H2O 6.80 g KCl 0.66 g CaCl2 x 2 H2O 1.47 g KBr 0.09 g Selenite-tungstate solution (see medium 385) 1.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg KH2PO4 0.20 g NH4Cl 0.25 g NaHCO3 2.50 g Na-lactate 2.30 g Vitamin solution (see medium 141) 10.00 ml Seven vitamins solution (see medium 503) 1.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except dihydrogenphosphate, ammonium chloride, bicarbonate, lactate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Dispense under same gas atmosphere in culture vessels and autoclave. Add dihydrogenphosphate, ammonium chloride, bicarbonate, lactate, vitamins and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas or 80% N2 and 20% CO2 gas mixture (bicarbonate). The vitamin solutions should be sterilized by filtration. Adjust the final pH of the medium to 7.2 - 7.5. For DSM 15769 replace lactate as substrate with 0.45 g/l 3-phenylpropionate, which is added to the medium after autoclaving from a sterile anoxic stock solution prepared under 100% N2. artifical seawater medium Carrine Blank DSM strains: DSMZ Medium 1011.3 Carrine Blank DSMZ Medium 1011.3 -< for DSM 28671 Similar to DSMZ Medium 1011, except that dinitrogen and carbon dioxide are omitted DSM 28671 is described as Thiomicrospira crunogena strain SP-41 (strain is not in Tax Browser yet). Thiomicrospira crunogena http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1011.pdf 1011. MJ MEDIUM NaCl 30.00 g K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.18 g KCl 0.33 g NH4Cl 0.25 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml NaHCO3 1.50 g Na2S2O3 x 5 H2O 1.50 g Vitamin solution (see medium 141) 10.00 ml Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, thiosulfate and vitamins), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Dispense under same gas atmosphere in culture vessels (up to a volume of 20%) and autoclave. Add bicarbonate, thiosulfate and vitamins to the autoclaved medium from sterile anoxic stock solutions. Solutions of vitamins and thiosulfate are sterilized by filtration and stored under N2, whereas the solution of bicarbonate is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Adjust the final pH of the medium to 6.7. After inoculation pressurize vessels to 0.5 bar overpressure with 80% N2 and 20% CO2 gas mixture and add sterile air in an amount that is equivalent to a volume of 20% of the headspace. For DSM 23290 supplement medium with 2.00 g/l NaNO3. After inoculation pressurize vessels to 1 bar overpressure with 80% N2 and 20% CO2 gas mixture. Do not add sterile air! For DSM 24660 omit thiosulfate and supplement medium with 4.00 g/l yeast extract and 4.00 g/l Trypton peptone. After autoclaving the medium is reduced with 0.30 g/l Na2S x 9 H2O added from a sterile anoxic stock solution (3% w/v) prepared under 100% N2 gas and the pH adjusted to 6.5. After inoculation pressurize vessels to 1 bar overpressure with 80% N2 and 20% CO2 gas mixture. Do not add sterile air! For DSM 28671 omit pressurizing vials with 0.5 bar overpressure of 80% N2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 1011.2 Similar to DSMZ Medium 1011, except that sodium thiosulfate and air (oxygen) are omitted. Yeast extract, tryptone, and sodium sulfide is added. Finally, the pH is made slightly more acidic (6.5). http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1011.pdf 1011. MJ MEDIUM NaCl 30.00 g K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.18 g KCl 0.33 g NH4Cl 0.25 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml NaHCO3 1.50 g Na2S2O3 x 5 H2O 1.50 g Vitamin solution (see medium 141) 10.00 ml Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, thiosulfate and vitamins), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Dispense under same gas atmosphere in culture vessels (up to a volume of 20%) and autoclave. Add bicarbonate, thiosulfate and vitamins to the autoclaved medium from sterile anoxic stock solutions. Solutions of vitamins and thiosulfate are sterilized by filtration and stored under N2, whereas the solution of bicarbonate is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Adjust the final pH of the medium to 6.7. After inoculation pressurize vessels to 0.5 bar overpressure with 80% N2 and 20% CO2 gas mixture and add sterile air in an amount that is equivalent to a volume of 20% of the headspace. For DSM 23290 supplement medium with 2.00 g/l NaNO3. After inoculation pressurize vessels to 1 bar overpressure with 80% N2 and 20% CO2 gas mixture. Do not add sterile air! For DSM 24660 omit thiosulfate and supplement medium with 4.00 g/l yeast extract and 4.00 g/l Trypton peptone. After autoclaving the medium is reduced with 0.30 g/l Na2S x 9 H2O added from a sterile anoxic stock solution (3% w/v) prepared under 100% N2 gas and the pH adjusted to 6.5. After inoculation pressurize vessels to 1 bar overpressure with 80% N2 and 20% CO2 gas mixture. Do not add sterile air! For DSM 28671 omit pressurizing vials with 0.5 bar overpressure of 80% N2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 1011.2 -< for DSM 24660 DSM 24660 is Thermotomaculum hydrothermale DSMZ Medium 1011.1 DSMZ Medium 1011.1 -< for DSM 23290 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1011.pdf 1011. MJ MEDIUM NaCl 30.00 g K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.18 g KCl 0.33 g NH4Cl 0.25 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml NaHCO3 1.50 g Na2S2O3 x 5 H2O 1.50 g Vitamin solution (see medium 141) 10.00 ml Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, thiosulfate and vitamins), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Dispense under same gas atmosphere in culture vessels (up to a volume of 20%) and autoclave. Add bicarbonate, thiosulfate and vitamins to the autoclaved medium from sterile anoxic stock solutions. Solutions of vitamins and thiosulfate are sterilized by filtration and stored under N2, whereas the solution of bicarbonate is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Adjust the final pH of the medium to 6.7. After inoculation pressurize vessels to 0.5 bar overpressure with 80% N2 and 20% CO2 gas mixture and add sterile air in an amount that is equivalent to a volume of 20% of the headspace. For DSM 23290 supplement medium with 2.00 g/l NaNO3. After inoculation pressurize vessels to 1 bar overpressure with 80% N2 and 20% CO2 gas mixture. Do not add sterile air! For DSM 24660 omit thiosulfate and supplement medium with 4.00 g/l yeast extract and 4.00 g/l Trypton peptone. After autoclaving the medium is reduced with 0.30 g/l Na2S x 9 H2O added from a sterile anoxic stock solution (3% w/v) prepared under 100% N2 gas and the pH adjusted to 6.5. After inoculation pressurize vessels to 1 bar overpressure with 80% N2 and 20% CO2 gas mixture. Do not add sterile air! For DSM 28671 omit pressurizing vials with 0.5 bar overpressure of 80% N2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 1011, except that sodium nitrate is added and air (oxygen) is omitted. DSM 23290 is Sulfurovum lithotrophicum Carrine Blank DSMZ Medium 1011 DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1011.pdf 1011. MJ MEDIUM NaCl 30.00 g K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.18 g KCl 0.33 g NH4Cl 0.25 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml NaHCO3 1.50 g Na2S2O3 x 5 H2O 1.50 g Vitamin solution (see medium 141) 10.00 ml Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, thiosulfate and vitamins), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Dispense under same gas atmosphere in culture vessels (up to a volume of 20%) and autoclave. Add bicarbonate, thiosulfate and vitamins to the autoclaved medium from sterile anoxic stock solutions. Solutions of vitamins and thiosulfate are sterilized by filtration and stored under N2, whereas the solution of bicarbonate is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Adjust the final pH of the medium to 6.7. After inoculation pressurize vessels to 0.5 bar overpressure with 80% N2 and 20% CO2 gas mixture and add sterile air in an amount that is equivalent to a volume of 20% of the headspace. For DSM 23290 supplement medium with 2.00 g/l NaNO3. After inoculation pressurize vessels to 1 bar overpressure with 80% N2 and 20% CO2 gas mixture. Do not add sterile air! For DSM 24660 omit thiosulfate and supplement medium with 4.00 g/l yeast extract and 4.00 g/l Trypton peptone. After autoclaving the medium is reduced with 0.30 g/l Na2S x 9 H2O added from a sterile anoxic stock solution (3% w/v) prepared under 100% N2 gas and the pH adjusted to 6.5. After inoculation pressurize vessels to 1 bar overpressure with 80% N2 and 20% CO2 gas mixture. Do not add sterile air! For DSM 28671 omit pressurizing vials with 0.5 bar overpressure of 80% N2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved MJ medium A minerals-salts, liquid medium containing sodium chloride, potassium phosphate, calcium chloride, magnesium sulfate, magnesium chloride, potassium chloride, ammonium chloride, ferrous ammonium sulfate, trace elements, sodium bicarbonate, sodium carbonate, and vitamins. Prepared under an atmosphere of dinitrogen, carbon dioxide, and air (oxygen). Carrine Blank DSMZ Medium 1011a http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1011a.pdf 1011a. MJ MEDIUM FOR THIOBACTER SUBTERRANEUS NaCl 3.00 g K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g MgSO4 x 7 H2O 0.34 g MgCl2 x 6 H2O 0.42 g KCl 0.33 g NH4Cl 0.25 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml NaHCO3 1.50 g Na2S2O3 x 5 H2O 1.50 g Vitamin solution (see medium 141) 10.00 ml Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, thiosulfate and vitamins), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Dispense under same gas atmosphere in culture vessels (up to a volume of 20%) and autoclave. Add bicarbonate, thiosulfate and vitamins to the autoclaved medium from sterile anoxic stock solutions. Solutions of vitamins and thiosulfate are sterilized by filtration and stored under 100% N2 gas, whereas the solution of bicarbonate is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Adjust the final pH of the medium to 6.7. After inoculation add sterile air in an amount that is equivalent to a volume of 50% of the headspace. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 1011, except that reduced amounts of sodium chloride, magnesium sulfate, and magnesium chloride are added. MJ medium for Thiobacter subterraneus Thiobacter subterraneus medium Carrine Blank DSM strains: DSMZ Medium 1012 An organic-rich, liquid medium containing nutrient broth medium, casamino acids, yeast extract, calcium chloride, and magnesium chloride. Carrine Blank DN broth DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1012.pdf 1012. DN BROTH Bacto Nutrient Broth (Difco 0003) 0.8 g Casamino acids 0.5 g Yeast extract 0.1 g CaCl2 x 2 H2O 0.3 g MgCl2 x 6 H2O 0.6 g Distilled water 1000.0 ml Dissolve ingredients except calcium and magnesium chloride and adjust pH of the medium to 7.2 with NaOH. After autoclaving add calcium and magnesium from sterile stock solutions. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 1014 Carrine Blank Thioalkalivirbio halophilus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1014.pdf 1014. THIOALKALIVIBRIO HALOPHILUS MEDIUM NaCl 175.00 g K2HPO4 1.50 g NH4Cl 0.50 g Trace element solution SL-4 (see medium 14) 1.00 ml MgCl2 x 6 H2O 0.20 g MgSO4 x 7 H2O 0.25 g Na2S2O3 5.00 g NaHCO3 4.00 g Distilled water 1000.00 ml Dissolve ingredients except magnesium chloride, magnesium sulfate, thiosulfate and bicarbonat. Fill solution in screw capped Erlenmeyer flasks (1/10 volume) and autoclave at 110˚C for 30 min. Add remaining compounds from sterile stock solutions and adjust pH of the medium to 8.0 - 8.5. © 2015 DSMZ GmbH - All rights reserved DSM strains: A minerals-salts, liquid medium containing sodium chloride, potassium phosphate, ammonium chloride, trace elements, magnesium chloride, magnesium sulfate, sodium thiosulfate, and sodium bicarbonate. Used for the cultivation of Thioalkalkalivibrio halophilus. DSMZ Medium 1015 An organic rich, solid medium containing yeast extract, peptone, glucose, and agar. DSM strains: Carrine Blank YPGA http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1015.pdf 1015. YPGA Yeast extract 7.0 g Bactopeptone 7.0 g Glucose 7.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.3. © 2007 DSMZ GmbH - All rights reserved trace elements solution SL-4 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium14.pdf 14. CLOSTRIDIUM FORMICOACETICUM MEDIUM D-Fructose 5.00 g Yeast extract 5.00 g K2HPO4 10.00 g NaHCO3 10.00 g Trace element solution SL-4 (see below) 10.00 ml Pyridoxine-HCl 1.00 mg Resazurin 1.00 mg Na-thioglycolate 0.75 g Agar, if necessary 15.00 g Distilled water 1000.00 ml Dissolve ingredients (except frucose and bicarbonate) and sparge medium with 100% N2 gas to make it anoxic. Add fructose from a sterile anoxic stock solution prepared under N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust final pH to 8.0. Trace element solution SL-4: EDTA 0.50 g FeSO4 x 7 H2O 0.20 g Trace element solution SL-6 (see medium 27) 100.00 ml Distilled water 900.00 ml First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL–6. © 2009 DSMZ GmbH - All rights reserved Carrine Blank A trace elements solution containing EDTA, ferrous sulfate, and trace elements solution SL-6. DSMZ Medium 14 Carrine Blank An organic rich, solid medium containing D-fructose, yeast extract, potassium phosphate, sodium bicarbonate, trace elements, pyridoxine hydrochloride, resazurin, sodium thioglycollate, and agar. Prepared under an atmosphere of nitrogen and carbon dioxide. Used for the cultivation of Clostridium formicoaceticum. DSM strains: Clostridium formicoaceticum medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium14.pdf 14. CLOSTRIDIUM FORMICOACETICUM MEDIUM D-Fructose 5.00 g Yeast extract 5.00 g K2HPO4 10.00 g NaHCO3 10.00 g Trace element solution SL-4 (see below) 10.00 ml Pyridoxine-HCl 1.00 mg Resazurin 1.00 mg Na-thioglycolate 0.75 g Agar, if necessary 15.00 g Distilled water 1000.00 ml Dissolve ingredients (except frucose and bicarbonate) and sparge medium with 100% N2 gas to make it anoxic. Add fructose from a sterile anoxic stock solution prepared under N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust final pH to 8.0. Trace element solution SL-4: EDTA 0.50 g FeSO4 x 7 H2O 0.20 g Trace element solution SL-6 (see medium 27) 100.00 ml Distilled water 900.00 ml First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL–6. © 2009 DSMZ GmbH - All rights reserved calf serum https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/fbs/other-sera/bovine-serum.html Gibco® bovine (aka calf serum) serum is collected from prime cattle with an age range of 12-36 months, but typically less than 24 months. All lots are processed and manufactured in New Zealand. Each lot of bovine serum is tested for its ability to support the growth of VERO cells over three subcultures. At each passage, the cells subcultured to the original cell-inoculation density. Results are compared to parallel results obtained using control growth medium containing a previously characterized reference serum. Bovine serum is available in a heat inactivated and non-heat inactivated format. In addition, we offer a donor bovine serum and a donor bovine serum with iron. HI serum is heated for 30 minutes at 56°C while mixing to inactivate the complement Donor bovine serum with iron contains a Ferric citrate complex at 6.0 mg/dl bovine serum new borne calf serum Carrine Blank A serum medium ingredient, derived from the serum (coagulated blood) from a young calf, less than 36 months old, of a slaughtered cow (Bos taurus). DSMZ Medium 503.19 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSM 24856 is Thermoanaerobaculum aquaticum Similar to DSMZ Medium 503, except sodium nitrate, yeast extract, and sodium pyruvate are added as substrates. DSMZ Medium 503.19 -< for DSM 24856 Carrine Blank DSMZ Medium 503.20 DSMZ Medium 503.20 -< for DSM 24984 Similar to DSMZ Medium 503, except sodium acetate and disodium hydrogen arsenate are added as substrates. Carrine Blank DSM 24984 is Seleniivibrio woodruffii http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved trace elements solution for DSMZ Medium 1007 Carrine Blank A trace elements solution containing EDTA, copper chloride, ferrous sulfate, zinc sulfate, nickel chloride, cobalt chloride, sodium molybdate, and manganese chloride. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1007.pdf 1007. MINERAL MEDIUM KNO3 250.0 mg KH2PO4 100.0 mg MgSO4 x 7 H2O 50.0 mg CaCl2 x 2 H2O 10.0 mg Trace elements 1.0 ml Distilled water 1000.0 ml Trace elements: EDTA 5.00 g CuCl2 x 5 H2O 0.10 g FeSO4 x 7 H2O 2.00 g ZnSO4 x 7 H2O 0.10 g NiCl2 x 6 H2O 0.02 g CoCl2 x 6 H2O 0.20 g Na2MoO4 0.03 g MnCl2 x 4 H2O 0.03 g Distilled water 1000.00 ml Final pH 5.5-6.0. DSM 15672 may be grown on either 0.5-1.0% methanol or under a gas phase of 20% methane in air. DSM 15673 supplied from the DSMZ has been grown on methanol. DSM 16984 has been grown on 20% methane in air. DSM 24478, 24479, 24480, 24481, 24492, 24493 and 24527 have been grown on 50% methane in air. Strains should be grown with shaking when grown on methane. © 2012 DSMZ GmbH - All rights reserved charcoal filtered seawater An undefined inorganic chemical mixture comprised of seawater that has been passed through a charcoal filter. Carrine Blank charcoal filter Carrine Blank A water filter that uses charcoal to filter a liquid. artificial seawater An inorganic salts solution that mimics sea water. Carrine Blank synthetic seawater marine salts Instant Ocean sea salt Carrine Blank A defined inorganic chemical mixture made from a commercially available synthetic sea salt (Instant Ocean® sea salt). From: Instant Ocean Synthetic Sea Salt MSDS AQUARIUM SYSTEMS INC -- INSTANT OCEAN SYNTHETIC SEA SALT -- 6810-00N009578 ===================== Product Identification ===================== Product ID:INSTANT OCEAN SYNTHETIC SEA SALT MSDS Date:11/17/1986 FSC:6810 NIIN:00N009578 MSDS Number: BCSTP === Responsible Party === Company Name:AQUARIUM SYSTEMS INC Address:8141 TYLER BOULEVARD City:MENTOR State:OH ZIP:44060 Country:US Info Phone Num:216-255-1997 Emergency Phone Num:216-255-1997 CAGE:56932 === Contractor Identification === Company Name:AQUARIUM SYSTEMS INC Address:8141 TYLER BOULEVARD Box:City:MENTOR State:OH ZIP:44060 Phone:216-255-1997 CAGE:56932 ============= Composition/Information on Ingredients ============= Ingred Name:CALCIUM CHLORIDE CAS:10043-52-4 RTECS #:EV9800000 Fraction by Wt: Other REC Limits: OSHA PEL: ACGIH TLV: Ingred Name:POTASSIUM CHLORIDE CAS:7447-40-7 RTECS #:TS8050000 Fraction by Wt: Other REC Limits: OSHA PEL: ACGIH TLV: Ingred Name:SODIUM SULFATE CAS:7757-82-6 RTECS #:WE1650000 Fraction by Wt: Other REC Limits: OSHA PEL: ACGIH TLV: Ingred Name:NACL COMMON SALT CAS:7647-14-5 RTECS #:VZ4725000 Fraction by Wt: 70% Other REC Limits: OSHA PEL: ACGIH TLV: Ingred Name:MAGNESIUM CHLORIDE CAS:7786-30-3 RTECS #:OM2800000 Fraction by Wt: Other REC Limits: OSHA PEL: ACGIH TLV: ===================== Hazards Identification ===================== LD50 LC50 Mixture: Routes of Entry: Inhalation:YES Skin:NO Ingestion:NO Reports of Carcinogenicity:NTP:NO IARC:NO OSHA:NO Health Hazards Acute and Chronic:CHRONIC TOXICITY:MAY BE A MILD IRRITANT.ROUTE OF MOST DETRIMENTAL EXPOS:INHALATION,TARGET ORGANS:MUCUS MEMBRANES.SKIN,EYES. Explanation of Carcinogenicity:N/A Effects of Overexposure:NOT DETERMINED Medical Cond Aggravated by Exposure: ======================= First Aid Measures ======================= First Aid:EYE & SKIN CONTACT: RINSE WITH FRESH H*2O.INGESTION:DRINK WATER TO DILUTE.INHAL:CONTROL OF DUST TO COMFORT LEVELS IN WORK AREAS. ===================== Fire Fighting Measures ===================== Extinguishing Media:NON FLAMMABLE Fire Fighting Procedures: Unusual Fire/Explosion Hazard:NONE ================== Accidental Release Measures ================== Spill Release Procedures:SWEEP UP DRY & DISCARD.IF WET MOP UP & RINSE DOWN DRAIN. Neutralizing Agent: ====================== Handling and Storage ====================== Handling and Storage Precautions:STORE IN COOL,DRY AREA TO PREVENT MOISTURE PICK-UP. Other Precautions:NO SPECIAL PRECAUTIONS. ============= Exposure Controls/Personal Protection ============= Respiratory Protection:N/R Ventilation:N/R Protective Gloves:ADVISABLE.OVEREXPOS MAY CAUSE DRY SKIN Eye Protection:SAFETY GLASSES W/ SIDE SHIELDS Other Protective Equipment: Work Hygienic Practices:NORMAL GOOD HOUSEKEEPING PRACTICES Supplemental Safety and Health STATE:SOLID ================== Physical/Chemical Properties ================== HCC:N1 Boiling Pt:B.P. Text:NA Vapor Pres:NA Spec Gravity:1.2 pH:8.3-3% Solubility in Water:35GM/100ML Appearance and Odor:WHITE MIX,POWDER/CRYSTAL,ODORLESS Percent Volatiles by Volume: Corrosion Rate:NO ================= Stability and Reactivity Data ================= Stability Indicator/Materials to Avoid:YES Stability Condition to Avoid: Hazardous Decomposition Products:NONE Conditions to Avoid Polymerization: ==================== Disposal Considerations ==================== Waste Disposal Methods:DISPOSE OF IAW ALL FEDERAL,STATE & LOCAL REGULATIONS. Disclaimer (provided with this information by the compiling agencies): This information is formulated for use by elements of the Department of Defense. The United States of America in no manner whatsoever, expressly or implied, warrants this information to be accurate and disclaims all liability for its use. Any person utilizing this document should seek competent professional advice to verify and assume responsibility for the suitability of this information to their particular situation. Instant Ocean® sea salt DSMZ Medium 9 Carrine Blank An organic-rich, solid medium comprised of Baker's yeast, calcium chloride, vitamin B12 (cobalamin), and agar. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium9.pdf 9. VY/2 AGAR Baker's yeast 5.00 g CaCl2 x 2 H2O 1.36 g Vitamin B12 0.50 mg Agar (Difco) 15.00 g Distilled water 1000.00 ml Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50˚C (use pH-indicator paper). For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium MD1 (per liter: casiton 3.0 g; calciumchloride dihydrate 0.7 g; magnesiumsulphate heptahydrate 2.0 g) to the vial with freeze dried material. For DSM 11116 reduce amount of vitamin B12 to 0.05 mg/l. © 2007 DSMZ GmbH - All rights reserved VY/2 agar DSMZ Medium 2 Carrine Blank Bacillus pasteurii medium DSM strains: 276 Sporosarcina pasteurii 1 Sporosarcina pasteurii 317 Sporosarcina ureae L.E.1.1 320 Sporosarcina ureae L.E.P.4 2280 Sporosarcina ureae Gibson 229 40726 Sporosarcina ureae 2305 Sporosarcina ureae 2306 Sporosarcina ureae 2307 Sporosarcina ureae (none are sequenced) An organic-rich, solid microbiological culture medium containing meat extract, peptone, agar, and urea. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium2.pdf 2. BACILLUS PASTEURII MEDIUM To medium 1 add 20 g/l urea before autoclaving. Do not adjust pH; pH raises to about 8 due to heat degraded urea. For sporulation enhancement add 10 mg/l MnSO4 x H2O. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 3 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium3.pdf 3. AZOTOBACTER MEDIUM Glucose 5.00 g Mannitol 5.00 g CaCl2 x 2 H2O 0.10 g MgSO4 x 7 H2O 0.10 g Na2MoO4 x 2 H2O 5.00 mg K2HPO4 0.90 g KH2PO4 0.10 g FeSO4 x 7 H2O 0.01 g CaCO3 5.00 g Agar 15.00 g Distilled water 950.00 ml Adjust pH to 7.3. Sterilize glucose and mannitol separately (in 50 ml H2O) and add to the medium after autoclaving. Calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies. © 2007 DSMZ GmbH - All rights reserved Azotobacter medium DSM strains: 375 Azomonas agilis 89 Azomonas agilis 721 Azomonas macrocytogenes 722 Azomonas macrocytogenes 376 Azorhizophilus paspali 388 Azorhizophilus paspali 391 Azorhizophilus paspali 400 Azorhizophilus paspali 2283 Azorhizophilus paspali 88 Azorhizophilus paspali 2284 Azotobacter armeniacus 282 Azotobacter beijerinckii 373 Azotobacter beijerinckii 381 Azotobacter beijerinckii 1041 Azotobacter beijerinckii 281 Azotobacter chroococcum 328 Azotobacter chroococcum 368 Azotobacter chroococcum 369 Azotobacter chroococcum 374 Azotobacter chroococcum 397 Azotobacter chroococcum 393 Azotobacter chroococcum 398 Azotobacter chroococcum 2286 Azotobacter chroococcum 2288 Azotobacter nigricans subsp. nigricans 279 Azotobacter vinelandii 332 Azotobacter vinelandii 366 Azotobacter vinelandii 382 Azotobacter vinelandii 389 Azotobacter vinelandii 390 Azotobacter vinelandii 395 Azotobacter vinelandii 399 Azotobacter vinelandii 576 Azotobacter vinelandii 720 Azotobacter vinelandii 2289 Azotobacter vinelandii 2290 Azotobacter vinelandii 85 Azotobacter vinelandii 86 Azotobacter vinelandii 87 Azotobacter vinelandii 13529 Azotobacter vinelandii 44106 Saccharomonospora cyanea NA-134 An organic-rich, solid medium containing glucose, mannitol, calcium chloride, magnesium sulfate, sodium molybdate, potassium phosphate, ferrous sulfate, calcium carbonate, and agar. Carrine Blank DSMZ Medium 6 DSM strains: 277 Bacillus fastidiosus 278 Bacillus fastidiosus 280 Bacillus fastidiosus 283 Bacillus fastidiosus 324 Bacillus fastidiosus 325 Bacillus fastidiosus 326 Bacillus fastidiosus 1301 Bacillus fastidiosus 1305 Bacillus fastidiosus 72 Bacillus fastidiosus 73 Bacillus fastidiosus 76 Bacillus fastidiosus 77 Bacillus fastidiosus 78 Bacillus fastidiosus 82 Bacillus fastidiosus 83 Bacillus fastidiosus 91 Bacillus fastidiosus 1306 Bacillus sp. 1302 Bacillus sp. 1303 Bacillus sp. 1304 Bacillus sp. 43292 Mycobacterium vaccae 43113 Mycobacterium vaccae Carrine Blank Allantoin mineral medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium6.pdf 6. ALLANTOIN MINERAL MEDIUM K2HPO4 0.8 g KH2PO4 0.2 g MgSO4 x 7 H2O 0.5 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 0.01 g MnSO4 x H2O 1.0 mg Allantoin 20.0 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved A minerals-salts, solid culture medium comprised of potassium phospate, magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, allantoin, and agar. With the mix of potassium phosphates, the pH should be about 7.4. DSMZ Medium 7 DSM strains: An organic rich, solid medium containing glucose, peptone, yeast extract, and agar. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium7.pdf 7. ANCYLOBACTER - SPIROSOMA MEDIUM Glucose 1.0 g Peptone 1.0 g Yeast extract 1.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0 For DSM 27188 use washed agar and adjust to pH 5.0-5.5. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Ancylobacter-Spirosoma medium DSMZ Medium 7.1 DSM 27188 is not in StrainInfo, TaxBrowser, or www.dsmz.de. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium7.pdf 7. ANCYLOBACTER - SPIROSOMA MEDIUM Glucose 1.0 g Peptone 1.0 g Yeast extract 1.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0 For DSM 27188 use washed agar and adjust to pH 5.0-5.5. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 7.1 -< for DSM 27188 Similar to DSMZ Medium 7, except washed agar is used instead of agar and the pH is reduced to 5.0-5.5. washed agar Agar that has been purified/treated through the process of washing. Carrine Blank DSMZ Medium 8 DSM strains: An organic-rich, solid medium containing glucose, peptone, yeast extract, calcium carbonate, and agar. Bacillus racemilacticus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium8.pdf 8. BACILLUS "RACEMILACTICUS" MEDIUM Glucose 5.0 g Peptone 5.0 g Yeast extract 5.0 g CaCO3 5.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 6.8. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 220.2 Similar to DSMZ Medium 220, except methanol is added. DSM 5746 is Methylobacillus DSMZ Medium 220.2 -< for DSM 5746 From: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium220.pdf 220. CASO AGAR (Merck 105458) Peptone from casein 15.0 g Peptone from soymeal 5.0 g NaCl 5.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid Cm131). For strain DSM 21449 und DSM 21450 NaCl 10.0 g/L MnSO4 10.0 mg/L For strain DSM 5746 Methanol © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 220.1 Similar to DSMZ Medium 220, except sodium chloride is increased and manganese sulfate is added. DSM 21449 is not in www.dsmz.de, TaxBrowser, or StainInfo. DSM 21450 is not in www.dsmz.de, TaxBrowser, or StainInfo Carrine Blank From: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium220.pdf 220. CASO AGAR (Merck 105458) Peptone from casein 15.0 g Peptone from soymeal 5.0 g NaCl 5.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid Cm131). For strain DSM 21449 und DSM 21450 NaCl 10.0 g/L MnSO4 10.0 mg/L For strain DSM 5746 Methanol © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 220.1 -< for DSM 21449 and DSM 21450 DSMZ Medium 11 Carrine Blank An organic rich, liquid culture medium containing casein peptone (casitone), meat extract, yeast extract, glucose, Tween 80 (polysorbate 80), potassium phosphate, sodium acetate, ammonium citrate, magnesium sulfate, and manganese sulfate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium11.pdf 11. MRS MEDIUM Casein peptone, tryptic digest 10.00g Meat extract 10.00g Yeast extract 5.00g Glucose 20.00 g Tween 80 1.00g K2HPO4 2.00g Na-acetate 5.00g (NH4)2 citrate 2.00g MgSO4 x 7 H2O 0.20 g MnSO4 x H2O 0.05g Distilled water 1000.00 ml Adjust pH to 6.2 - 6.5. © 2007 DSMZ GmbH - All rights reserved MRS medium DSM strains: DSMZ Medium 12 soil extract medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium12.pdf 12. SOIL EXTRACT MEDIUM Sterilize 400.0 g of air-dried garden soil (with high content of organic matter) in 1000 ml tap water for one hour at 121˚C. Allow it to sediment for a few hours at room temperature. Centrifuge the supernatant. Add 15.0 g agar per 1000 ml to the clear supernatant solution thus obtained. Adjust pH to 6.8 - 7.0 and sterilize. © 2007 DSMZ GmbH - All rights reserved A liquid culture medium comprised of a soil extract and agar. DSM strains: DSMZ Medium 13 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium13.pdf 13. BACILLUS ACIDOCALDARIUS MEDIUM Solution A: Yeast extract 1.00g (NH4)2SO4 0.20g MgSO4 x 7 H2O 0.50g CaCl2 x 2 H2O 0.25g KH2PO4 0.60g Distilled water 500.00 ml Adjust pH to 3.0 - 4.0. Solution B: Glucose 1.00g Agar 20.00 g Distilled water 500.00 ml Sterilize solution A and B separately at 121˚C for 15 min. Cool to 50˚C combine. © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of yeast extract, ammonium sulfate, magnesium sulfate, calcium chloride, potassium phosphate, glucose, and agar. DSM strains: Carrine Blank Bacillus acidocaldarium medium DSMZ Medium 21 An organic-rich, liquid culture medium comprised of glucose, peptone, and yeast extract. Sarcina medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium21.pdf 21. SARCINA MEDIUM Glucose 30.0g Peptone 5.0g Yeast extract 5.0 g Distilled water 100.00 ml Adjust pH to 6.0. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: DSMZ Medium 25 Rhodopseudomonas globiformis medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium25.pdf 25. RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM Yeast extract 0.25 g Mannitol 1.50 g Na-gluconate 0.50 g KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NH4Cl 0.40 g NaCl 0.40 g CaCl2 x 2 H2O 0.05 g Trace element solution SL-6 (see medium 27) 1.00 ml Distilled water 1000.00 ml Adjust pH to 4.9, then add: Fe(III) citrate solution (0.1% in H2O) 5.00 ml Biotin solution (0.002% in H2O) 1.00 ml p-Aminobenzoic acid solution (0.01% in H2O) 1.00 ml Sterilize in screw-capped bottles. Cool to room temperature; to each 100 ml medium add 0.2 ml of a sterile 10% Na2S203 solution. Incubate in the light using a tungsten lamp. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank An organic-rich, liquid culture medium comprised of yeast extract, mannitol, sodium gluconate, potassium phosphate, magnesium sulfate, ammonium chloride, sodium chloride, calcium chloride, trace elements, ferric citrate, biotin, p-aminobenzoic acid (4-aminobenzoic acid), and sodium thiosulfate. DSMZ Medium 26 DSM strains: Similar to DSMZ Medium 27, except the yeast extract concentration is reduced and the pH is reduced to 5.7. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium26.pdf 26. ACID RHODOSPIRILLACEAE MEDIUM Use medium 27 and reduce the yeast extract concentration to 0.2 g/l. Adjust pH to 5.7. Incubate in the light using a tungsten lamp. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Acid Rhodospirillaceae medium DSMZ Medium 28 Pfennig's medium 1 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium28.pdf 28. PFENNIG'S MEDIUM I Solution A: CaCl2 x 2 H2O 0.25 g Yeast extrakt 0.25 g Distilled water 460.00 ml Fill 10x 46ml in 100 ml screw-cap bottles. Bubble with N2/CO2 and autoclave 121°C 15 min. (For marine or estuarine isolates add 100.0 g NaCl to this solution and increase the MgSO4 x 7 H2O to 15.0 g). Solution B: Na2S x 9 H2O 2.00 g Distilled water 135.00 ml Prepare in a screw-cap bottle, bubble with N2 to replace air, close tightly and autoclave. Solution C: NaHCO3 1.50 g H2O 50.00 ml Bubble with CO2 and filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle. Solution D Resazurin (0,1%) 0.5 ml Distilled water 450.00 ml Autoclave in a cotton-stoppered Erlenmeyer flask with an outlet tube for medium, connected to a glass outlet at the bottom of the vessel and has, at the other end, a silicon rubber tube with a pinch cock and a bell for aseptic dispensing of the medium into bottles. Cool to room temperature under an atmosphere of N2/CO2 in an ice bath. Solution E: Ammonium chloride 0.35 g Ammonium acetate 0.25 g Pyruvic acid sodium salt 0.25 g Dextrose 0.25 g MgSO4x7H2O 0.50 g KCL 0.35 g KH2PO4 0.35 g Trace element solution SL-12 B 1.00 ml Distilled water 25 ml Filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle. Solution F: Vitamin B12 0.01 g Distilled water 100.00 ml Filter sterilized Trace element solution SL-12 B: Distilled water 1000.00 ml Na2-EDTA 3.00 g FeSO4 x 7 H2O 1.10 g CoCl2 x 6 H2O 190.00 mg MnCl2 x 2 H2O 50.00 mg ZnCl2 42.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 18.00 mg H3BO3 300.00 mg CuCl2 x 2 H2O 2.00 mg Adjust pH to 6.0. Mix solution D, C and E. Bubble with CO2 in an ice bath under sterile conditions. Fill 50 ml in each bottle of solution A. Before using add 4 ml solution B and 0.1 ml solution F. Adjust the pH with filter-sterilised 1M Na2CO3 to 7.1-7.3. Fill in sterile, N2 gassed screw-cap tubes under N2 gas. During the first 24 h, the iron of the medium precipitates in the form of black flocks. No other sediment should arise in the otherwise clear medium. Feed periodically with neutralized 3% solution of sodium sulfide to replenish sulfide and with other supplement solutions (see Ref. 3365). Neutralized sulfide solution: Distilled water 100.00 ml Na2S x 9 H2O 3.00 g The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121˚C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle. Appearance of a yellow colour indicates the drop of pH to about 8. The solution should be stirred continuously to avoid precipitation of elemental sulfur. The final solution should be clear and is yellow in colour. © 2007 DSMZ GmbH - All rights reserved A minerals-salts, liquid culture medium comprised of calcium chloride, yeast extract, sodium sulfide, sodium bicarbonate, resazurin, ammonium chloride, ammonium acetate, pyruvic acid sodium salt (sodium pyruvate), dextrose (D-glucose), magnesium sulfate, potassium chloride, potassium phosphate, trace elements, vitamin B12 (cobalamin), sodium carbonate, and sulfuric acid. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: Carrine Blank DSMZ Medium 28a DSM strains: Thiorhodococcus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium28a.pdf 28a. THIORHODOCOCCUS MEDIUM supplement medium 28 with NaCl (2%), MgCl2 x 6 H2O (0.1%) and sodium thiosulfate (0.05%). Incubate at 500 to 1000 lux light intensity. © 2007 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 28, except sodium chloride, magnesium chloride, and sodium thiosulfate are added. Carrine Blank DSMZ Medium 28b Similar to DSMZ Medium 28, except sodium chloride, sodium thiosulfate, and magnesium chloride are added. pH is increased to 7.5 Carrine Blank Thiorhodococcus medium II DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium28b.pdf 28b. THIORHODOCOCCUS MEDIUM II Supplement Medium 28 with NaCl 1.50 % Na2S2O3 x 5 H2O 0.05 % MgCl x 6 H2O 0.25 % Adjust to pH 7.5, anaerobic incubation under nitrogen/carbon dioxide atmosphere; light (see medium 28). © 2008 DSMZ GmbH - All rights reserved DSMZ Medium 29 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium29.pdf 29. PFENNIG'S MEDIUM II Solution A: CaCl2 x 2 H2O 0.25 g Yeast extrakt 0.25 g Distilled water 460.00 ml Fill 10x 46ml in 100 ml screw-cap bottles. Bubble with N2/CO2 and autoclave 121°C 15 min. (For marine or estuarine isolates add 100.0 g NaCl to this solution and increase the MgSO4 x 7 H2O to 15.0 g). Solution B: Na2S x 9 H2O 2.00 g Distilled water 135.00 ml Prepare in a screw-cap bottle, bubble with N2 to replace air, close tightly and autoclave. Solution C: NaHCO3 1.50 g H2O 50.00 ml Bubble with CO2 and filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle. Solution D Resazurin (0,1%) 0.5 ml Distilled water 450.00 ml Autoclave in a cotton-stoppered Erlenmeyer flask with an outlet tube for medium, connected to a glass outlet at the bottom of the vessel and has, at the other end, a silicon rubber tube with a pinch cock and a bell for aseptic dispensing of the medium into bottles. Cool to room temperature under an atmosphere of N2/CO2 in an ice bath. Solution E: Ammonium chloride 0.35 g Ammonium acetate 0.25 g Pyruvic acid sodium salt 0.25 g Dextrose 0.25 g MgSO4x7H2O 0.50 g KCL 0.35 g KH2PO4 0.35 g Trace element solution SL-10 B 1.00 ml Distilled water 25 ml Filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle. Solution F: Vitamin B12 0.01 g Distilled water 100.00 ml Filter sterilized Trace element solution SL-10 B: Distilled water 1000.0 ml HCl (25%) 7.7 ml FeSO4 x 7 H2O 1.5 g ZnCl2 70.0 mg MnCl2 x 4 H2O 100.0 mg H3BO3 300.0 mg CoCl2 x 6 H2O 190.0 mg CuCl2 x 2 H2O 2.0 mg NiCl2 x 6 H2O 24.0 mg Na2MoO4 x 2 H2O 36.0 mg Mix solution D, C and E. Bubble with CO2 in an ice bath under sterile conditions. Fill 50 ml in each bottle of solution A. Before using add 4 ml solution B and 0.1 ml solution F. Adjust the pH with filter-sterilised 1M Na2CO3 to 6.8 -7.1. Fill in sterile, N2 gassed screw-cap tubes under N2 gas. During the first 24 h, the iron of the medium precipitates in the form of black flocks. No other sediment should arise in the otherwise clear medium. Feed periodically with neutralized 3% solution of sodium sulfide to replenish sulfide and with other supplement solutions (see Ref. 3365). Neutralized sulfide solution: Distilled water 100.00 ml Na2S x 9 H2O 3.00 g The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121˚C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle. Appearance of a yellow colour indicates the drop of pH to about 8. The solution should be stirred continuously to avoid precipitation of elemental sulfur. The final solution should be clear and is yellow in colour. © 2007 DSMZ GmbH - All rights reserved A minerals-salts, liquid culture medium comprised of calcium chloride, yeast extract, sodium sulfide, sodium bicarbonate, resazurin, ammonium chloride, ammonium acetate, pyruvic acid sodium salt (sodium pyruvate), dextrose (D-glucose), magnesium sulfate, potassium chloride, potassium phosphate, trace elements, vitamin B12 (cobalamin), sodium carbonate, and sulfuric acid. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: Pfennig's medium II DSMZ Medium 29a http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium29a.pdf 29a. MEDIUM FOR CHLOROBIUM FERROOXIDANS From medium 29, omit sulfide, add 10 mM bicarbonate, 10 mM FeSO4 and 2mM acetate. Adjust pH to 6.8. At 25˚C in light the incubation time is about 4 weeks. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 29, except sodium sulfide is omitted and sodium bicarbonate, ferrous sulfate, and sodium acetate are added, and the pH is decreased to 6.8. Medium for Chlorobium ferrooxidans DSM strains: Chlorobium ferrooxidans medium trace elements solution SL-12 B A trace elements solution containing disodium EDTA, ferrous sulfate, cobalt chloride, manganese chloride, zinc chloride, nickel chloride, sodium molybdate, boric acid, and copper chloride. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium28.pdf 28. PFENNIG'S MEDIUM I Solution A: CaCl2 x 2 H2O 0.25 g Yeast extrakt 0.25 g Distilled water 460.00 ml Fill 10x 46ml in 100 ml screw-cap bottles. Bubble with N2/CO2 and autoclave 121°C 15 min. (For marine or estuarine isolates add 100.0 g NaCl to this solution and increase the MgSO4 x 7 H2O to 15.0 g). Solution B: Na2S x 9 H2O 2.00 g Distilled water 135.00 ml Prepare in a screw-cap bottle, bubble with N2 to replace air, close tightly and autoclave. Solution C: NaHCO3 1.50 g H2O 50.00 ml Bubble with CO2 and filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle. Solution D Resazurin (0,1%) 0.5 ml Distilled water 450.00 ml Autoclave in a cotton-stoppered Erlenmeyer flask with an outlet tube for medium, connected to a glass outlet at the bottom of the vessel and has, at the other end, a silicon rubber tube with a pinch cock and a bell for aseptic dispensing of the medium into bottles. Cool to room temperature under an atmosphere of N2/CO2 in an ice bath. Solution E: Ammonium chloride 0.35 g Ammonium acetate 0.25 g Pyruvic acid sodium salt 0.25 g Dextrose 0.25 g MgSO4x7H2O 0.50 g KCL 0.35 g KH2PO4 0.35 g Trace element solution SL-12 B 1.00 ml Distilled water 25 ml Filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle. Solution F: Vitamin B12 0.01 g Distilled water 100.00 ml Filter sterilized Trace element solution SL-12 B: Distilled water 1000.00 ml Na2-EDTA 3.00 g FeSO4 x 7 H2O 1.10 g CoCl2 x 6 H2O 190.00 mg MnCl2 x 2 H2O 50.00 mg ZnCl2 42.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 18.00 mg H3BO3 300.00 mg CuCl2 x 2 H2O 2.00 mg Adjust pH to 6.0. Mix solution D, C and E. Bubble with CO2 in an ice bath under sterile conditions. Fill 50 ml in each bottle of solution A. Before using add 4 ml solution B and 0.1 ml solution F. Adjust the pH with filter-sterilised 1M Na2CO3 to 7.1-7.3. Fill in sterile, N2 gassed screw-cap tubes under N2 gas. During the first 24 h, the iron of the medium precipitates in the form of black flocks. No other sediment should arise in the otherwise clear medium. Feed periodically with neutralized 3% solution of sodium sulfide to replenish sulfide and with other supplement solutions (see Ref. 3365). Neutralized sulfide solution: Distilled water 100.00 ml Na2S x 9 H2O 3.00 g The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121˚C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle. Appearance of a yellow colour indicates the drop of pH to about 8. The solution should be stirred continuously to avoid precipitation of elemental sulfur. The final solution should be clear and is yellow in colour. © 2007 DSMZ GmbH - All rights reserved trace elements solution SL-10 B A trace elements solution containing hydrochloric acid (hydrogen chloride), ferrous sulfate, zinc chloride, manganese chloride, boric acid, cobalt chloride, copper chloride, nickel chloride, and sodium molybdate. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium29.pdf 29. PFENNIG'S MEDIUM II Solution A: CaCl2 x 2 H2O 0.25 g Yeast extrakt 0.25 g Distilled water 460.00 ml Fill 10x 46ml in 100 ml screw-cap bottles. Bubble with N2/CO2 and autoclave 121°C 15 min. (For marine or estuarine isolates add 100.0 g NaCl to this solution and increase the MgSO4 x 7 H2O to 15.0 g). Solution B: Na2S x 9 H2O 2.00 g Distilled water 135.00 ml Prepare in a screw-cap bottle, bubble with N2 to replace air, close tightly and autoclave. Solution C: NaHCO3 1.50 g H2O 50.00 ml Bubble with CO2 and filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle. Solution D Resazurin (0,1%) 0.5 ml Distilled water 450.00 ml Autoclave in a cotton-stoppered Erlenmeyer flask with an outlet tube for medium, connected to a glass outlet at the bottom of the vessel and has, at the other end, a silicon rubber tube with a pinch cock and a bell for aseptic dispensing of the medium into bottles. Cool to room temperature under an atmosphere of N2/CO2 in an ice bath. Solution E: Ammonium chloride 0.35 g Ammonium acetate 0.25 g Pyruvic acid sodium salt 0.25 g Dextrose 0.25 g MgSO4x7H2O 0.50 g KCL 0.35 g KH2PO4 0.35 g Trace element solution SL-10 B 1.00 ml Distilled water 25 ml Filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle. Solution F: Vitamin B12 0.01 g Distilled water 100.00 ml Filter sterilized Trace element solution SL-10 B: Distilled water 1000.0 ml HCl (25%) 7.7 ml FeSO4 x 7 H2O 1.5 g ZnCl2 70.0 mg MnCl2 x 4 H2O 100.0 mg H3BO3 300.0 mg CoCl2 x 6 H2O 190.0 mg CuCl2 x 2 H2O 2.0 mg NiCl2 x 6 H2O 24.0 mg Na2MoO4 x 2 H2O 36.0 mg Mix solution D, C and E. Bubble with CO2 in an ice bath under sterile conditions. Fill 50 ml in each bottle of solution A. Before using add 4 ml solution B and 0.1 ml solution F. Adjust the pH with filter-sterilised 1M Na2CO3 to 6.8 -7.1. Fill in sterile, N2 gassed screw-cap tubes under N2 gas. During the first 24 h, the iron of the medium precipitates in the form of black flocks. No other sediment should arise in the otherwise clear medium. Feed periodically with neutralized 3% solution of sodium sulfide to replenish sulfide and with other supplement solutions (see Ref. 3365). Neutralized sulfide solution: Distilled water 100.00 ml Na2S x 9 H2O 3.00 g The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121˚C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle. Appearance of a yellow colour indicates the drop of pH to about 8. The solution should be stirred continuously to avoid precipitation of elemental sulfur. The final solution should be clear and is yellow in colour. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 39 Similar to DSMZ Medium 28, except that additional yeast extract (twice the amount) is added. DSM strains: Pfennig's medium I with yeast extract http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium39.pdf 39. PFENNIG'S MEDIUM I WITH YEAST EXTRACT To medium 28 add 0.05% yeast extract. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 38 An organic-rich, liquid culture medium comprised of tryptone, yeast extract, potassium phosphate, and sodium sulfide. Prepared under an atmosphere of dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium38.pdf 38. CLOSTRIDIUM STICKLANDII MEDIUM Tryptone 20.00 g Yeast extract 10.00 g K2HPO4 1.04 g KH2PO4 0.68 g Na2S x 9 H2O 0.15 g Distilled water 1000.00 ml Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under N2. © 2014 DSMZ GmbH - All rights reserved Clostridium sticklandii medium DSM strains: Carrine Blank DSMZ Medium 37 Spirillum medium DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium37.pdf 37. SPIRILLUM MEDIUM Peptone (BD) 10.000 g Succinic acid 1.000 g (NH4)2SO4 1.000 g MgSO4 x 7 H20 1.000 g FeCL3 x 6 H20 0.002 g MnSO4 x H20 0.002 ml Distilled water 1000.0 ml Adjust pH to 6.8 © 2007 DSMZ GmbH - All rights reserved A liquid, organic rich culture medium comprised of peptone, succinic acid, ammonium sulfate, magnesium sulfate, ferric trichloride, and manganese sulfate. DSMZ Medium 36 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium36.pdf 36. THIOBACILLUS THIOPARUS MEDIUM (NH4)2SO4 0.10 g K2HPO4 4.00 g KH2PO4 4.00 g MgSO4 x 7 H2O 0.10 g CaCl2 0.10 g FeCl3 x 6 H2O 0.02 g MnSO4 x H2O 0.02 g Na2S2O3 x 5 H2O 10.00 g Distilled water 1000.00 ml Dissolve all the ingredients in distilled water and adjust the pH to 6.6. Sterilize by autoclaving at 115˚C for 20 min. For solid medium add 12.00 g/l purified agar after adjustment of pH. For DSM 18181: Adjust pH of medium to 7.5 and add 1.00 g/l yeast extract. For solid medium add 20.00 g/l purified agar. © 2015 DSMZ GmbH - All rights reserved Carrine Blank A minerals-salts, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric chloride, manganese sulfate, and sodium thiosulfate. DSM strains: Thiobacillus thioparus medium DSMZ Medium 36.1 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium36.pdf 36. THIOBACILLUS THIOPARUS MEDIUM (NH4)2SO4 0.10 g K2HPO4 4.00 g KH2PO4 4.00 g MgSO4 x 7 H2O 0.10 g CaCl2 0.10 g FeCl3 x 6 H2O 0.02 g MnSO4 x H2O 0.02 g Na2S2O3 x 5 H2O 10.00 g Distilled water 1000.00 ml Dissolve all the ingredients in distilled water and adjust the pH to 6.6. Sterilize by autoclaving at 115˚C for 20 min. For solid medium add 12.00 g/l purified agar after adjustment of pH. For DSM 18181: Adjust pH of medium to 7.5 and add 1.00 g/l yeast extract. For solid medium add 20.00 g/l purified agar. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 36, except yeast extract is added and the pH is increased to 7.5. DSMZ Medium 36.1 -< for DSM 18181 DSM 18181 is Thiomonas bhubaneswarensis Carrine Blank DSMZ Medium 35a A minerals-salts, liquid culture medium containing ammonium chloride, potassium phosphate, magnesium chloride, calcium chloride, sodium thiosulfate, and yeast extract. Thiomonas intermedia medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium35a.pdf 35a. THIOMONAS INTERMEDIA MEDIUM NH4Cl 0.1 g KH2PO4 3.0 g MgCl2 x 6 H2O 0.1 g CaCl2 0.1 g Na2S2O3 x 5 H2O 5.0 g Yeast extract 1.0 g Distilled water 1000.0 ml Adjust pH to 5.5-6.0 and autoclave. Add sodium thiosulfate after autoclaving from a sterile stock solution sterilized by filtration. Best growth is observed in the syneresis water of slant agar tubes (20.0 g/l agar). For DSM 22701 change amount of yeast extract to 0.5 g/l and of Na-thiosulfate to 2.5 g/l. © 2011 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 35a.1 Similar to DSMZ Medium 35a, except that the amounts of yeast extract and thiosulfate are reduced. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium35a.pdf 35a. THIOMONAS INTERMEDIA MEDIUM NH4Cl 0.1 g KH2PO4 3.0 g MgCl2 x 6 H2O 0.1 g CaCl2 0.1 g Na2S2O3 x 5 H2O 5.0 g Yeast extract 1.0 g Distilled water 1000.0 ml Adjust pH to 5.5-6.0 and autoclave. Add sodium thiosulfate after autoclaving from a sterile stock solution sterilized by filtration. Best growth is observed in the syneresis water of slant agar tubes (20.0 g/l agar). For DSM 22701 change amount of yeast extract to 0.5 g/l and of Na-thiosulfate to 2.5 g/l. © 2011 DSMZ GmbH - All rights reserved DSM 22701 is Thiomonas arsenitoxydans DSMZ Medium 35a.1 -< for DSM 22701 DSMZ Medium 35 Carrine Blank DSM strains: Acidithiobacillus thiooxidans medium A minerals-salts, liquid culture medium containing ammonium chloride, potassium phosphate, magnesium chloride, calcium chloride, and powdered sulfur (elemental sulfur). http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium35.pdf 35. ACIDITHIOBACILLUS THIOOXIDANS MEDIUM NH4Cl 0.10 g KH2PO4 3.00 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.14 g Sulfur, powdered 10.00 g Distilled water 1000.00 ml Dissolve all ingredients, except the sulfur, in distilled water and adjust the pH to 4.2, then autoclave. Place the sulfur in screw-capped tubes or bottles and sterilize by autoclaving at 112˚C for 15 min. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. For DSM 612 supplement medium with 0.10 g/l yeast extract (sterilized separately). © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 35.1 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium35.pdf 35. ACIDITHIOBACILLUS THIOOXIDANS MEDIUM NH4Cl 0.10 g KH2PO4 3.00 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.14 g Sulfur, powdered 10.00 g Distilled water 1000.00 ml Dissolve all ingredients, except the sulfur, in distilled water and adjust the pH to 4.2, then autoclave. Place the sulfur in screw-capped tubes or bottles and sterilize by autoclaving at 112˚C for 15 min. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. For DSM 612 supplement medium with 0.10 g/l yeast extract (sterilized separately). © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 35.1 -< for DSM 612 Similar to DSMZ Medium 35, except yeast extract is added. DSM 612 is Thiobacillus sp. DSM 612 DSMZ Medium 34 DSM strains: Caryophanon latum medium An organic-rich, liquid culture medium containing yeast extract, trypticase peptone, soy peptone, sodium acetate, sodium glutamate, thiamine hydrochloride, biotin, potassium phosphate, magnesium sulfate, and tris buffer. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium34.pdf 34. CARYOPHANON LATUM MEDIUM Yeast extract 2.00 g Trypticase (BBL) 2.00 g Soya peptone 2.00 g Na-acetate 1.00 g Na-glutamate 0.10 g Thiamine-HCl x 2 H2O 0.20 mg Biotin 0.05 mg K2HPO4 1.00 g MgSO4 x 7 H2O 0.27 g Tris/HCl-buffer 10mM, pH 7.8 1000.00 ml Adjust pH to 7.8. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 31 An organic-rich, solid microbiological culture medium containing peptone, meat extract, agar, sodium bicarbonate, and sodium carbonate. Carrine Blank alkaline nutrient agar http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium31.pdf 31. ALKALINE NUTRIENT AGAR Same as medium 1. After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7. Na-sesquicarbonate solution: NaHCO3 4.2 g Na2CO3 anhydrous 5.3 g Distilled water 100.0 ml © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 48 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium48.pdf 48. TRYPTONE THIOGLYCOLATE MEDIUM K2HPO4 5.450 g KH2PO4 1.200 g MgSO4 x 7 H2O 0.025 g CaCl2 x 2 H2O 0.015 g FeSO4 x 7 H2O 0.010 g MnCl2 x 4 H2O 2.000 mg CoCl2 x 6 H2O 2.500 mg Na2MoO4 x 2 H2O 2.500 mg Glucose 20.000 g Peptone 2.000 g Tryptone 2.000 g Yeast extract 6.000 g Na-thioglycolate 0.500 g Distilled water 1000.000 ml Adjust pH to 7.5. Dissolve the glucose in 50 ml distilled water and sterilize it separately. © 2007 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium sulfate, calcium chloride, ferrous sulfate, manganese chloride, cobalt chloride, sodium molybdate, glucose, peptone, tryptone, yeast extract, and sodium thioglycolate. tryptone thioglycolate medium Carrine Blank DSMZ Medium 49 C/10 medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium49.pdf 49. C/10 MEDIUM Casitone 3.00 g CaCl2 x 2 H2O 1.36 g Agar 15.00 g Distilled water 1000.00 ml Adjust pH to 7.2 before adding agar. © 2007 DSMZ GmbH - All rights reserved DSM strains: An organic rich, solid culture medium comprised of casitone, calcium chloride, and agar. DSMZ Medium 40 Pfennig's medium II with salt http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium40.pdf 40. PFENNIG'S MEDIUM II WITH SALT To medium 29 add 1% NaCl. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank Similar to DSMZ Medium 29, except that sodium chloride is added. DSMZ Medium 52 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium52.pdf 52. CLOSTRIDIUM KLUYVERI MEDIUM (MODIFIED) K-acetate 10.00 g K2HPO4 0.31 g KH2PO4 0.23 g NH4Cl 0.25 g MgSO4 x 7 H2O 0.20 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 1.00 g Resazurin 0.50 mg Ethanol 20.00 ml NaHCO3 2.50 g Seven vitamin solution (see medium 503) 1.00 ml L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Dissolve all ingredients (except ethanol, bicarbonate, reducing agents and vitamins) in distilled water and boil for 1 min. After cooling to room temperature under 80% N2 and 20% CO2 gas atmosphere, add ethanol, dispense in Hungate tubes and autoclave. After autoclaving, add bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere and vitamins from an anoxic and filter-sterilized stock solution. Before inoculation, add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Adjust pH of medium to 6.8 – 7.0, if necessary. If reduction of medium is not complete after inoculation, add 10 – 20 mg/l sodium dithionite from a 5% w/v solution freshly prepared under N2 and filter sterilized. © 2009 DSMZ GmbH - All rights reserved Carrine Blank A minerals-salts, liquid culture medium containing potassium acetate, potassium phosphate, ammonium chloride, magnesium sulfate, trace elements, selenite-tungstate solution, yeast extract, resazurin, ehtanol, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: Clostridium kluyveri medium (modified) DSMZ Medium 51 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium51.pdf 51. SPHAEROTILUS MEDIUM Beef extract (Lab Lemco, Oxoid) 5.0 g Agar (if necessary) 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0. Prepare sterile agar slants by autoclaving. Cool the slants in a sloping position. Cover solid slants with 2 ml sterile tap water. Inoculate into the covering tap water and incubate at 20 to 25˚C for at least 48 h. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Sphaerotilus medium DSM strains: An organic-rich, liquid culture medium comprised of beef extract and agar. DSMZ Medium 53 An organic-rich, solid culture medium comprised of casein peptone tryptic digest (casitone), yeast extract, glucose, sodium chloride, and agar. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium53.pdf 53. CORYNEBACTERIUM AGAR Casein peptone, tryptic digest 10.0 g Yeast extract 5.0 g Glucose 5.0 g NaCl 5.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2 - 7.4. © 2007 DSMZ GmbH - All rights reserved Corynebacterium agar Carrine Blank DSMZ Medium 54 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium54.pdf 54. GLUCOSE YEAST EXTRACT MEDIUM Glucose 20.0 g Yeast extract 10.0 g CaCO3 (light precipitate) 20.0 g Distilled water 1000.0 ml Dissolve ingredients (except carbonate, which remains solid) and sparge medium for 30 min. with 100% N2 gas. Dispense under stirring to keep carbonate suspended in anoxic vessels and autoclave. For solid medium add 17.0 g/l agar. © 2010 DSMZ GmbH - All rights reserved DSM strains: 30201 Agrobacterium 50012 Burkholderia glathei 50014 Burkholderia glathei 50401 Burkholderia 53 Clostridium beijerinckii 6422 Clostridium beijerinckii 6423 Clostridium beijerinckii 6566 Clostridium butyricum 525 Clostridium pasteurianum DSM 525 = ATCC 6013 526 Clostridium pasteurianum 9989 Clostridium pasteurianum 523 Clostridium 3873 Pantoea cypripedii 30182 Pantoea cypripedii 30183 Pantoea cypripedii 30184 Pectobacterium atrosepticum 30185 Pectobacterium atrosepticum 30186 Pectobacterium atrosepticum 50069 Pseudomonas aeruginosa 5024 Pseudomonas aeruginosa 50259 Pseudomonas asplenii 50260 Pseudomonas cichorii 7231 Pseudomonas cichorii 50275 Pseudomonas fuscovaginae 50276 Pseudomonas marginalis 50282 Pseudomonas marginalis 50286 Pseudomonas savastanoi 80287 Pseudomonas savastanoi 50298 Pseudomonas savastanoi 50317 Pseudomonas 50291 Pseudomonas 1241 Pseudomonas syringae 1242 Pseudomonas syringae 1856 Pseudomonas syringae 10604 Pseudomonas syringae DSM 10604 50255 Pseudomonas syringae pv. atrofaciens str. DSM 50255 50256 Pseudomonas syringae 50261 Pseudomonas syringae 50277 Pseudomonas syringae 50281 Pseudomonas syringae 50292 Pseudomonas syringae 50293 Pseudomonas syringae 50302 Pseudomonas syringae 50303 Pseudomonas syringae 50312 Pseudomonas syringae 50315 Pseudomonas syringae 50335 Pseudomonas syringae 50336 Pseudomonas syringae 30200 Agrobacterium rhizogenes 1805 Sphingomonas echinoides ATCC 14820 14405 Stenotrophomonas rhizophila 50853 Xanthomonas arboricola 50854 Xanthomonas arboricola 50850 Xanthomonas axonopodis 1050 Xanthomonas campestris 1526 Xanthomonas campestris 2405 Xanthomonas campestris 2406 Xanthomonas campestris 2407 Xanthomonas campestris 3586 Xanthomonas campestris pv. campestris str. ATCC 33913 50852 Xanthomonas campestris 1220 Xanthomonas citri pv. malvacearum 50857 Xanthomonas hortorum 50858 Xanthomonas hortorum 50859 Xanthomonas hortorum 50855 Xanthomonas hyacinthi 1350 Xanthomonas sp. DSM 1350 3851 Xanthomonas An organic-rich, liquid culture medium comprised of glucose, yeast extract, and calcium carbonate. The pKa of calcium carbonate is 9.0, therefore the pH of the solution will be about pH 9.0. Carrine Blank glucose yeast extract medium salt solution for DSMZ Medium 58 Carrine Blank An inorganic salts solution comprised of calcium chloride, magnesium sulfate, potassium phosphate, sodium bicarbonate, and sodium chloride. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium58.pdf 58. BIFIDOBACTERIUM MEDIUM Casein peptone, tryptic digest 10.00 g Yeast extract 5.00 g Meat extract 5.00 g Bacto Soytone 5.00 g Glucose 10.00 g K2HPO4 2.00 g MgSO4 x 7 H2O 0.20 g MnSO4 x H20 0.05 g Tween 80 1.00 ml NaCl 5.00 g Cysteine-HCl x H2O 0.50 g Salt solution (see below) 40.00 ml Resazurin (25 mg/100ml) 4.00 ml Distilled water 950.00 ml The cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave. Salt solution: CaCl2 x 2 H2O 0.25 g MgSO4 x 7 H2O 0.50 g K2HPO4 1.00 g KH2PO4 1.00 g NaHCO3 10.00 g NaCl 2.00 g Distilled water 1000.00 ml © 2008 DSMZ GmbH - All rights reserved DSMZ Medium 55 Carrine Blank DSM strains: 416 Cupriavidus necator 418 Cupriavidus necator 422 Cupriavidus necator fructose mineral medium A minerals-salts, liquid culture medium comprised of disodium hydrogen phosphate, potassium dihydrogen phosphate, ammonium chloride, magnesium sulfate, trace elements, calcium chloride, ferric ammonium citrate, and fructose. The pH of this medium should be about 7.2, given the ratio of sodium phosphate and potassium phosphate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium55.pdf 55. FRUCTOSE MINERAL MEDIUM Solution A: Na2HPO4 x 12 H2O 9.000 g KH2PO4 1.500 g NH4Cl 1.000 g MgSO4 x 7 H2O 0.200 g Trace element solution 0.300 ml SL-6 (see medium 27) Distilled water 500.000 ml Solution B: CaCl2 x 2 H2O 0.010 g Ferric ammonium citrate 0.005 g Distilled water 300.000 ml Solution C: Fructose 5.000 g Distilled water 200.000 ml Autoclave solutions 1 and 2 separately, filter sterilize solution C. Add 1.5% agar if necessary. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 56 trypticase starch agar http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium56.pdf 56. TRYPTICASE STARCH AGAR Trypticase soy broth 30.0 g Yeast extract 2.0 g Soluble starch 1.0 g Agar 15.0 g Distilled water 1000.0 g © 2007 DSMZ GmbH - All rights reserved An organic-rich, solid culture medium comprised of trypticase soy broth (here, tryptic soy agar), yeast extract, and soluble starch. Carrine Blank DSMZ Medium 58 DSM strains: 22365 Aeriscardovia aeriphila 17774 Alloscardovia criceti DSM 17774 17775 Alloscardovia criceti 17776 Alloscardovia criceti 21503 Alloscardovia omnicolens DSM 21503 17244 Anaerofustis stercorihominis DSM 17244 17241 Anaerotruncus colihominis DSM 17241 22766 Bifidobacterium actinocoloniiforme DSM 22766 20083 Bifidobacterium adolescentis ATCC 15703 20086 Bifidobacterium adolescentis 20087 Bifidobacterium adolescentis DSM 20087 24849 Bifidobacterium adolescentis 26737 Bifidobacterium aesculapii 26738 Bifidobacterium aesculapii 20098 Bifidobacterium angulatum DSM 20098 = JCM 7096 20225 Bifidobacterium angulatum 20105 Bifidobacterium animalis subsp. lactis ATCC 27536 20104 Bifidobacterium animalis subsp. animalis ATCC 25527 10140 Bifidobacterium animalis subsp. lactis DSM 10140 20089 Bifidobacterium asteroides DSM 20089 20431 Bifidobacterium asteroides 23969 Bifidobacterium biavatii DSM 23969 20215 Bifidobacterium bifidum 20082 Bifidobacterium bifidum 20239 Bifidobacterium bifidum 20456 Bifidobacterium bifidum ATCC 29521 = JCM 1255 = DSM 20456 22767 Bifidobacterium bohemicum DSM 22767 19703 Bifidobacterium bombi DSM 19703 20432 Bifidobacterium boum DSM 20432 20213 Bifidobacterium breve DSM 20213 = JCM 1192 20091 Bifidobacterium breve 23973 Bifidobacterium callitrichos DSM 23973 16992 Bifidobacterium catenulatum DSM 16992 = JCM 1194 = LMG 11043 Bifidobacterium catenulatum 20224 Bifidobacterium choerinum 20434 Bifidobacterium choerinum DSM 20434 20216 Bifidobacterium coryneforme DSM 20216 20435 Bifidobacterium cuniculi DSM 20435 20084 Bifidobacterium dentium 20221 Bifidobacterium dentium 20436 Bifidobacterium dentium JCM 1195 = DSM 20436 20093 Bifidobacterium gallicum DSM 20093 = LMG 11596 20214 Bifidobacterium indicum LMG 11587 = DSM 20214 21854 Bifidobacterium kashiwanohense JCM 15439 = DSM 21854 28807 Bifidobacterium sp. LMC 13 20088 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088 20090 Bifidobacterium longum subsp. infantis 20090 Bifidobacterium longum subsp. infantis 20218 Bifidobacterium longum subsp. infantis 20097 Bifidobacterium longum subsp. longum 20219 Bifidobacterium longum subsp. longum 20211 Bifidobacterium longum subsp. suis DSM 20211 20220 Bifidobacterium magnum 20222 Bifidobacterium magnum DSM 20222 6492 Bifidobacterium merycicum DSM 6492 6493 Bifidobacterium merycicum 6494 Bifidobacterium merycicum 20102 Bifidobacterium minimum DSM 20102 21395 Bifidobacterium mongoliense DSM 21395 27321 Bifidobacterium moukalabense DSM 27321 20438 Bifidobacterium pseudocatenulatum DSM 20438 = JCM 1200 = LMG 10505 20439 Bifidobacterium pseudocatenulatum 20092 Bifidobacterium pseudolongum subsp. globosum DSM 20092 20094 Bifidobacterium pseudolongum subsp. pseudolongum 20095 Bifidobacterium pseudolongum subsp. pseudolongum 20099 Bifidobacterium pseudolongum subsp. pseudolongum DSM 20099 22366 Bifidobacterium psychraerophilum DSM 22366 20433 Bifidobacterium pullorum DSM 20433 23975 Bifidobacterium reuteri DSM 23975 6489 Bifidobacterium ruminantium DSM 6489 6490 Bifidobacterium ruminantium 6491 Bifidobacterium ruminantium 6531 Bifidobacterium saeculare DSM 6531 = LMG 14934 6532 Bifidobacterium saeculare 6533 Bifidobacterium saeculare 23967 Bifidobacterium saguini DSM 23967 13734 Bifidobacterium scardovii JCM 12489 = DSM 13734 23968 Bifidobacterium stellenboschense 20096 Bifidobacterium subtile DSM 20096 17755 Bifidobacterium thermacidophilum subsp. porcinum DSM 17755 15837 Bifidobacterium thermacidophilum subsp. thermacidophilum DSM 15837 Bifidobacterium thermophilum 20209 Bifidobacterium thermophilum 20210 Bifidobacterium thermophilum DSM 20210 20212 Bifidobacterium thermophilum DSM 20212 17777 Bifidobacterium tsurumiense DSM 17777 17778 Bifidobacterium tsurumiense 17779 Bifidobacterium tsurumiense 20653 Lactobacillus aviarius subsp. araffinosus DSM 20653 20656 Lactobacillus aviarius subsp. araffinosus 20654 Lactobacillus aviarius subsp. aviarius 20655 Lactobacillus aviarius subsp. aviarius DSM 20655 20584 Lactobacillus crispatus DSM 20584 = JCM 1185 20461 Megasphaera cerevisiae 20462 Megasphaera cerevisiae DSM 20462 16981 Megasphaera paucivorans 17042 Megasphaera sueciensis 21655 Mobiluncus curtisii subsp. holmesii ATCC 35242 10105 Parascardovia denticolens DSM 10105 = JCM 12538 10106 Parascardovia denticolens 24661 Pectinatus brassicae 20466 Pectinatus cerevisiiphilus 20467 Pectinatus cerevisiiphilus 20762 Pectinatus cerevisiiphilus 20465 Pectinatus frisingensis 16980 Pectinatus haikarae 20764 Pectinatus sp. DSM 20764 24742 Pseudoscardovia radai 24744 Pseudoscardovia suis 16840 Roseburia faecis M72/1 16839 Roseburia hominis A2-183 10107 Scardovia inopinata 10108 Scardovia inopinata 20757 Selenomonas lacticifex 18934 Sharpea azabuensis DSM 18934 17536 Streptococcus castoreus DSM 17536 7200 Zymophilus paucivorans 7201 Zymophilus paucivorans 20756 Zymophilus paucivorans 7198 Propionispira raffinosivorans 7199 Propionispira raffinosivorans 20765 Propionispira raffinosivorans DSM 20765 Bifidobacterium medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium58.pdf 58. BIFIDOBACTERIUM MEDIUM Casein peptone, tryptic digest 10.00 g Yeast extract 5.00 g Meat extract 5.00 g Bacto Soytone 5.00 g Glucose 10.00 g K2HPO4 2.00 g MgSO4 x 7 H2O 0.20 g MnSO4 x H20 0.05 g Tween 80 1.00 ml NaCl 5.00 g Cysteine-HCl x H2O 0.50 g Salt solution (see below) 40.00 ml Resazurin (25 mg/100ml) 4.00 ml Distilled water 950.00 ml The cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave. Salt solution: CaCl2 x 2 H2O 0.25 g MgSO4 x 7 H2O 0.50 g K2HPO4 1.00 g KH2PO4 1.00 g NaHCO3 10.00 g NaCl 2.00 g Distilled water 1000.00 ml © 2008 DSMZ GmbH - All rights reserved Carrine Blank An organic-rich, liquid culture medium comprised of casein peptone, tryptic digest (casitone), yeast extract, meat extract, Bacto soytone (soy peptone), glucose, potassium phosphate, magnesium sulfate, manganese sulfate, tween 80 (polysorbate 80), sodium chloride, cysteine hydrochloride, salt solution and resazurin. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 57 Carrine Blank An organic-rich, liquid culture medium containing tryptone, meat extract, yeast extract, tween 80 (polysorbate 80), sodium acetate, magnesium sulfate, magnesium sulfate, and casamino acids. AAM medium DSM strains: 20253 Lactobacillus rennini DSM 20253 20254 Lactobacillus rennini http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium57.pdf 57. AAM MEDIUM Tryptone 5.0 g Meat extract 5.0 g Yeast extract 7.0 g Tween 80 1.0 ml Na-acetate 2.5 g MgSO4 x 7 H2O 200.0 mg MnSO4 x H2O 50.0 mg Casamino acids 7.0 g Distilled water 1000.0 ml Adjust pH to 5.4. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 59 DSM strains: 20252 Oenococcus oeni DSM 20252 = AWRIB129 20255 Oenococcus oeni 20257 Oenococcus oeni Leuconostoc oenos medium Carrine Blank An organic-rich, liquid culture medium comprised of casein peptone tryptic digest (casitone), yeast extract, glucose, fructose, magnesium sulfate, manganese sulfate, ammonium citrate, tween 80 (polysorbate 80), filtered tomato juice, and cysteine hydrochloride. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium59.pdf 59. LEUCONOSTOC OENOS MEDIUM Casein peptone, tryptic digest 10.00 g Yeast extract 5.00 g Glucose 10.00 g Fructose 5.00 g MgSO4 x 7 H2O 0.20 g MnSO4 x H2O 0.05 g (NH4) citrate 3.50 g Tween 80 1.00 ml Tomato juice, filtered 100.00 ml Cysteine-HCl x H2O 0.50 g Distilled water 900.00 ml Adjust pH to 4.8. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 60 An organic-rich, liquid culture medium comprised of magnesium sulfate, ammonium sulfate, ferrous ammonium sulfate, sodium molybdate, sodium selenite, tryptone, yeast extract, resazurin, potassium phosphate, glucose, sodium bicarbonate, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of carbon dioxide and dinitrogen. Clostridium thermoaceticum medium Carrine Blank DSM strains: 521 Moorella thermoacetica 11768 Moorella thermoacetica http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium60.pdf 60. CLOSTRIDIUM THERMOACETICUM MEDIUM Solution A: MgSO4 x 7 H2O 0.10 g (NH4)2SO4 0.50 g Fe(NH4)2(SO4)2 0.04 g Na2MoO4 x 2 H2O 2.40 mg Na2SeO3 x 5 H2O 0.15 mg Tryptone 5.00 g Yeast extract 5.00 g Resazurin 0.50 mg Distilled water 800.00 ml Solution B: K2HPO4 7.00 g KH2PO4 4.50 g Distilled water 50.00 ml Solution C: Glucose 18.00 g Distilled water 50.00 ml Solution D: NaHCO3 10.00 g Distilled water 100.00 ml Solution E: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution F: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Prepare solution A under 100% CO2 gas atmosphere. Distribute and autoclave under same gas atmosphere. Autoclave separately solutions B, C, E and F under 100% N2, and solution D under 100% CO2 gas atmosphere. After sterilization combine all solutions aseptically. Adjust pH of the complete medium to 6.9 if necessary. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 61 An organic-rich, liquid culture medium comprised of tryptone, sucrose, yeast extract, ferrous sulfate, sodium sulfite, sodium thiosulfate, and resazurin. Prepared under an atmosphere of dinitrogen. Clostridium thermohydrosulfuricum medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium61.pdf 61. CLOSTRIDIUM THERMOHYDROSULFURICUM MEDIUM Tryptone 10.00 g Sucrose 10.00 g Yeast extract 2.00 g FeSO4 x 7 H2O 0.20 g Na2SO3 0.20 g Na2S2O3 x 5 H2O 0.08 g Resazurin 1.00 mg Distilled water 1000.00 ml Dissolve ingredients (except thiosulfate), adjust pH to 6.8 - 7.5 and sparge medium with 100% N2 gas to make it anoxic (30 – 45 min). Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add thiosulfate from a sterile anoxic stock solution prepared under N2. For DSM 8686 and DSM 8690 replace sucrose with D-xylose as substrate and adjust pH of medium to 5.2. For DSM 15493 replace sucrose with 3.0 g/l D-maltose. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: 2246 Thermoanaerobacter ethanolicus JW 200 12299 Thermoanaerobacter siderophilus SR4 15493 Thermoanaerobacter sp. 518-21 567 Thermoanaerobacter thermohydrosulfuricus 568 Thermoanaerobacter thermohydrosulfuricus 569 Thermoanaerobacter thermohydrosulfuricus 570 Thermoanaerobacter thermohydrosulfuricus 2247 Thermoanaerobacter thermohydrosulfuricus 7021 Thermoanaerobacter thermohydrosulfuricus 7022 Thermoanaerobacter thermohydrosulfuricus 26960 Thermoanaerobacter thermohydrosulfuricus WC1 14765 Thermoanaerobacterium bryantii 8686 Thermoanaerobacterium 8690 Thermoanaerobacterium 571 Thermoanaerobacterium thermosaccharolyticum DSM 571 572 Thermoanaerobacterium thermosaccharolyticum 573 Thermoanaerobacterium thermosaccharolyticum 869 Thermoanaerobacterium thermosaccharolyticum DSMZ Medium 61.1 Carrine Blank DSMZ Medium 61.1 -< for DSM 8686 and DSM 8690 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium61.pdf 61. CLOSTRIDIUM THERMOHYDROSULFURICUM MEDIUM Tryptone 10.00 g Sucrose 10.00 g Yeast extract 2.00 g FeSO4 x 7 H2O 0.20 g Na2SO3 0.20 g Na2S2O3 x 5 H2O 0.08 g Resazurin 1.00 mg Distilled water 1000.00 ml Dissolve ingredients (except thiosulfate), adjust pH to 6.8 - 7.5 and sparge medium with 100% N2 gas to make it anoxic (30 – 45 min). Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add thiosulfate from a sterile anoxic stock solution prepared under N2. For DSM 8686 and DSM 8690 replace sucrose with D-xylose as substrate and adjust pH of medium to 5.2. For DSM 15493 replace sucrose with 3.0 g/l D-maltose. © 2014 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 61, except that sucrose is omitted, D-xylose is added, and the pH is reduced to 5.2. DSM 8686 is Thermoanaerobacterium sp. DSM 8690 is Thermoanaerobacterium sp. Neither strains are in TaxBrowser DSMZ Medium 61.2 DSMZ 15493 is Thermoanaerobacter sp. 518-21 DSMZ Medium 61.2 -< for DSM 15493 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium61.pdf 61. CLOSTRIDIUM THERMOHYDROSULFURICUM MEDIUM Tryptone 10.00 g Sucrose 10.00 g Yeast extract 2.00 g FeSO4 x 7 H2O 0.20 g Na2SO3 0.20 g Na2S2O3 x 5 H2O 0.08 g Resazurin 1.00 mg Distilled water 1000.00 ml Dissolve ingredients (except thiosulfate), adjust pH to 6.8 - 7.5 and sparge medium with 100% N2 gas to make it anoxic (30 – 45 min). Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add thiosulfate from a sterile anoxic stock solution prepared under N2. For DSM 8686 and DSM 8690 replace sucrose with D-xylose as substrate and adjust pH of medium to 5.2. For DSM 15493 replace sucrose with 3.0 g/l D-maltose. © 2014 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 61, except that sucrose is omitted and D-maltose (beta-maltose) is added. DSMZ Medium 63.1 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium63.pdf 63. DESULFOVIBRIO MEDIUM (FRESHWATER) Solution A: K2HPO4 0.5 g NH4Cl 1.0 g Na2SO4 1.0 g CaCl2 x 2 H2O 0.1 g MgSO4 x 7 H2O 2.0 g Na-DL-lactate 2.0 g Yeast extract 1.0 g Resazurin 1.0 mg Distilled water 980.0 ml Solution B: FeSO4 x 7 H2O 0.5 g Distilled water 10.0 ml Solution C: Na-thioglycolate 0.1 g Ascorbic acid 0.1 g Distilled water 10.0 ml Bring solution A to the boil, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121˚C. For DSM 12838 supplement medium with 3.0 g/l meat extract. © 2015 DSMZ GmbH - All rights reserved DSM 12838 is Desulfomicrobium orale DSM 12838 DSMZ Medium 63.1 -< for DSM 12838 Similar to DSMZ Medium 63, except that meat extract is added. Carrine Blank DSMZ Medium 63 Desulfovibrio medium (freshwater) DSM strains: 24233 Desulfobaculum xiamenense 5918 Desulfomicrobium apsheronum 1742 Desulfomicrobium baculatum 1743 Desulfomicrobium baculatum 2555 Desulfomicrobium baculatum 4028 Desulfomicrobium baculatum DSM 4028 10707 Desulfomicrobium escambiense DSM 10707 4194 Desulfomicrobium macestii 12838 Desulfomicrobium orale DSM 12838 12927 Desulfomicrobium 15374 Desulfomicrobium 12929 Desulforhopalus 13351 Desulfosporosinus auripigmenti 765 Desulfosporosinus orientis DSM 765 7439 Desulfosporosinus orientis 11792 Desulfotomaculum australicum DSM 11792 4024 [Desulfotomaculum] guttoideum DSM 4024 6115 Desulfotomaculum kuznetsovii DSM 6115 12396 Desulfotomaculum luciae 574 Desulfotomaculum nigrificans DSM 574 575 Desulfotomaculum nigrificans 7434 Desulfotomaculum nigrificans 7435 Desulfotomaculum nigrificans 7436 Desulfotomaculum nigrificans 7437 Desulfotomaculum nigrificans 7438 Desulfotomaculum nigrificans 7453 Desulfotomaculum nigrificans 12395 Desulfotomaculum putei DSM 12395 2154 Desulfotomaculum ruminis DSM 2154 7452 Desulfotomaculum ruminis 6711 Desulfotomaculum 7440 Desulfotomaculum 7441 Desulfotomaculum 7442 Desulfotomaculum 7443 Desulfotomaculum 14812 Desulfotomaculum 15631 Desulfotomaculum 5813 Desulfotomaculum thermoacetoxidans 6193 Desulfotomaculum thermobenzoicum subsp. thermobenzoicum 2603 Desulfovibrio africanus subsp. africanus DSM 2603 23860 Desulfovibrio africanus subsp. uniflagellum 6133 Desulfovibrio alcoholivorans 12254 Desulfovibrio aminophilus DSM 12254 21064 Desulfovibrio arcticus 6830 Desulfovibrio burkinensis 11391 Desulfovibrio cuneatus DSM 11391 11392 Desulfovibrio cuneatus 642 Desulfovibrio desulfuricans subsp. desulfuricans DSM 642 1924 Desulfovibrio desulfuricans subsp. desulfuricans 4369 Desulfovibrio desulfuricans subsp. desulfuricans 6946 Desulfovibrio desulfuricans subsp. desulfuricans 9104 Desulfovibrio desulfuricans subsp. desulfuricans 12129 Desulfovibrio desulfuricans subsp. desulfuricans 3604 Desulfovibrio fructosivorans JJ 15450 Desulfovibrio idahonensis DSM 15450 15451 Desulfovibrio idahonensis 15121 Desulfovibrio indonesiensis 11275 Desulfovibrio intestinalis DSM 11275 11393 Desulfovibrio litoralis DSM 11393 6739 Desulfovibrio longus DSM 6739 1925 Desulfovibrio oxamicus 16681 Desulfovibrio paquesii DSM 16681 4141 Desulfovibrio simplex 496 Desulfovibrio 6605 Desulfovibrio 8712 Desulfovibrio 9266 Desulfovibrio 12803 Desulfovibrio 15744 Desulfovibrio 5308 Desulfovibrio termitidis HI1 11274 Desulfovibrio termitidis 15375 Desulfovibrio vulgaris 644 Desulfovibrio vulgaris str. Hildenborough 2119 Desulfovibrio vulgaris 6617 Desulfovibrio vulgaris 6618 Desulfovibrio vulgaris 6619 Desulfovibrio vulgaris 6621 Desulfovibrio vulgaris 6622 Desulfovibrio vulgaris 6623 Desulfovibrio vulgaris http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium63.pdf 63. DESULFOVIBRIO MEDIUM (FRESHWATER) Solution A: K2HPO4 0.5 g NH4Cl 1.0 g Na2SO4 1.0 g CaCl2 x 2 H2O 0.1 g MgSO4 x 7 H2O 2.0 g Na-DL-lactate 2.0 g Yeast extract 1.0 g Resazurin 1.0 mg Distilled water 980.0 ml Solution B: FeSO4 x 7 H2O 0.5 g Distilled water 10.0 ml Solution C: Na-thioglycolate 0.1 g Ascorbic acid 0.1 g Distilled water 10.0 ml Bring solution A to the boil, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121˚C. For DSM 12838 supplement medium with 3.0 g/l meat extract. © 2015 DSMZ GmbH - All rights reserved Desulfovibrio medium Carrine Blank A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium sulfate, calcium chloride, magnesium sulfate, sodium lactate, yeast extract, resazurin, ferrous sulfate, sodium thioglycolate, and ascorbic acid. Prepared under an atmosphere of dinitrogen. DSMZ Medium 63a A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium sulfate, calcium chloride, magnesium sulfate, sodium acetate, sodium pyruvate, yeast extract, resazurin, ferrous sulfate, sodium thioglycolate, and ascorbic acid. Prepared under an atmosphere of dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium63a.pdf 63a. DESULFOTOMACULUM SP. MEDIUM I Solution A: K2HPO4 0.5 g NH4Cl 1.0 g Na2SO4 1.0 g CaCl2 x 2 H2O 0.1 g MgSO4 x 7 H2O 2.0 g Na-acetate 2.0 g Na-pyruvate 5.0 g Yeast extract 1.0 g Resazurin 1.0 mg Distilled water 980.0 ml Solution B: FeSO4 x 7 H2O 0.5 g Distilled water 10.0 ml Solution C: Na-thioglycolate 0.1 g Ascorbic acid 0.1 g Distilled water 10.0 ml Bring solution A to the boil, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 6.8 with NaOH, and distribute under N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121˚C. Check medium pH after autoclaving and adjust to 6.5 - 7.0, if necessary. © 2014 DSMZ GmbH - All rights reserved Desulfotomaculum sp. medium I DSM strains: 6711 Desulfotomaculum Carrine Blank DSMZ Medium 63b Similar to DSMZ Medium 63, except that the medium is made up in filtered aged seawater. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium63b.pdf 63b. DESULFOVIBRIO MEDIUM (SEAWATER) Solution A: K2HPO4 0.5 g NH4Cl 1.0 g Na2SO4 1.0 g CaCl2 x 2 H2O 0.1 g MgSO4 x 7 H2O 2.0 g Na-DL-lactate 2.0 g Yeast extract 1.0 g Resazurin 1.0 mg Filtered aged sea water 980.0 ml Alternatively to filtered aged sea water bottled water from Biomaris GmbH supplemented with 15.0 g/l NaCl can be used. Solution B: FeSO4 x 7 H2O 0.5 g Distilled water 10.0 ml Solution C: Na-thioglycolate 0.1 g Ascorbic acid 0.1 g Distilled water 10.0 ml Bring solution A to the boil, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121˚C. © 2007 DSMZ GmbH - All rights reserved Desulfovibrio medium Desulfovibrio medium (seawater) DSM strains: none in BacDive Carrine Blank DSMZ Medium 545 TSB Carrine Blank An organic-rich, solid culture medium comprised of glucose, yeast extract, calcium carbonate, and agar. Tryptone soya broth DSM strains: 50262 50272 50307 50311 1049 50851 3849 3850 50861 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium54a.pdf 54a. REACTIVATION WITH LIQUID MEDIUM 545 Rehydrate and grow lyophilized cells from the ampoule in liquid medium 54 or 545. Subsequent subculturing may be carried out in liquid medium or on agar plates medium 54. 54. GLUCOSE YEAST EXTRACT AGAR Glucose 20.0 g Yeast extract 10.0 g CaCO3 (light precipitate) 20.0 g Agar, if required 17.0 g Distilled water 1000.0 ml 545. TRYPTONE SOYA BROTH (TSB) Peptone from casein 17.0 g Peptone from soymeal 3.0 g D(+)-Glucose 2.5 g NaCl 5.0 g K2HPO4 2.5 g Distilled water 1000.0 ml Adjust pH to 7.3. The same as Caso Bouillon (Merck or Oxoid, Germany). © 2007 DSMZ GmbH - All rights reserved filtered seawater Carrine Blank Undefined inorganic chemical mixture, derived from the filtering of natural seawater. whole blood A blood medium ingredient comprised of whole blood, which has not been treated or altered. Carrine Blank dried whole egg Microbiological medium ingredient, derived from chicken egg, where whole chicken eggs have been dried. Carrine Blank Instant Ocean An artificial seawater made using water and Instant Ocean® sea salt. Instant Ocean® Carrine Blank microbiological medium ingredient, derived from chicken egg Carrine Blank An undefined organic chemical mixture, derived from the eggs of chickens (Gallus gallus). Used for the cultivation of microorganisms. Bakers yeast An undefined organic chemical mixture comprised of dried yeast cells Saccharomyces cerevisiae), used to support the growth of microorganisms. Baker's yeast Carrine Blank DSMZ Medium 76 Carrine Blank Clostridium acidurici medium An organic-rich, liquid culture medium comprised of potassium hydroxide, potassium phosphate, uric acid, magnesium sulfate, calcium chloride, ferrous sulfate, trace elements, selenite-tungstate solution, yeast extract, sodium thioglycolate, sodium bicarbonate, and resazuring. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: 604 [Clostridium] acidurici 9a 10158 Gottschalkia acidurici 10159 Gottschalkia acidurici 10160 Gottschalkia acidurici 10161 Gottschalkia acidurici 605 Clostridium cylindrosporum DSM 605 10157 Clostridium cylindrosporum 10162 Clostridium cylindrosporum 10163 Clostridium cylindrosporum 10164 Clostridium cylindrosporum 1384 Clostridium purinilyticum 10156 Clostridium purinilyticum 1989 Eubacterium angustum 3244 Gallicola barnesae http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium76.pdf 76. CLOSTRIDIUM ACIDIURICI MEDIUM KOH 0.67 g K2HPO4 0.91 g Uric acid 2.00 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 15.00 mg FeSO4 x 7 H2O 6.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 1.00 g Na-thioglycolate 0.50 g NaHCO3 5.00 g Resazurin 1.00 mg Distilled water 1000.00 ml First dissolve KOH and K2HPO4 in water, then add uric acid and boil until the acid is dissolved. Cool medium to room temperature under 100% N2 gas atmosphere and add all other substances, except thioglycolate and bicarbonate. Dispense under same gas atmosphere into culture vessels and autoclave for 15 min at 121˚C. Then add thioglycolate (stock solution, autoclaved separately) and sodium bicarbonate (filter-sterilized stock solution prepared under 80% N2 and 20% CO2 gas atmosphere) and adjust pH of complete medium to 7.0 - 7.5. © 2007 DSMZ GmbH - All rights reserved filtered aged seawater Filtered seawater that has been aged (kept in the dark for 2-4 weeks). Carrine Blank DSMZ Medium 77 Carrine Blank An organic rich, liquid culture medium comprised of infusion from fresh liver (liver extract), peptone, potassium phosphate, and extracted liver tissue (dissected liver tissue). DSM strains: 601 Clostridium baratii 602 Clostridium baratii 46280 Clostridium paraputrificum 599 Clostridium sardiniense 600 Clostridium sardiniense 46282 Clostridium 46278 Clostridium sporogenes 46279 Clostridium sporogenes Liver broth http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium77.pdf 77. LIVER BROTH (Oxoid CM 77) Prepare the medium according to directions on the bottle under 100% N2 gas atmosphere. © 2015 DSMZ GmbH - All rights reserved http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0077&org=3&c=UK&lang=EN LIVER BROTH Code: CM0077 a liquid medium, containing liver particles, for the examination of foods for saccharolytic or putrefactive mesophilic and thermophilic anaerobes Typical Formula* gm/litre Infusion from fresh liver 23.0 Peptone 10.0 Potassium phosphate 1.0 Extracted liver tissue 30.0 pH 6.8 ± 0.2 DSMZ Medium 74 Carrine Blank THermus thermophilus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium74.pdf 74. THERMUS THERMOPHILUS MEDIUM Yeast extract (BD Difco) 4.0 g Proteose peptone Nr. 3 (BD Difco) 8.0 g NaCl 2.0 g Distilled water 1000.0 ml Adjust pH to 7.0. The original medium description specifies polypeptone, which appears to be no longer available. © 2012 DSMZ GmbH - All rights reserved DSM strains: 22914 Pseudoxanthomonas taiwanensis DSM 22914 16200 Thermus kawarayensis JCM 12314 579 Thermus thermophilus HB8 An organic rich, liquid culture medium comprised of yeast extract, proteose peptone, and sodium chloride. DSMZ Medium 75 Carrine Blank Trypticase phytone medium DSM strains: 587 Acinetobacter 588 Acinetobacter An organic-rich, liquid culture medium containing trypticase peptone, phytone peptone, sodium chloride, potassium phosphate, and glucose. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium75.pdf 75. TRYPTICASE PHYTONE MEDIUM Trypticase peptone 17.0 g Phytone peptone 3.0 g NaCl 5.0 g K2HPO4 2.5 g Glucose 2.5 g Distilled water 1000.0 ml Adjust pH to 7.3. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 73 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium73.pdf 73. MEDIUM FOR HALOPHILIC BACILLI Casamino acids 10.0 g Yeast extract 10.0 g NaCl 100.0 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved DSM strains: 578 Bacillus Medium for halophilic bacilli Carrine Blank An organic rich, liquid culture medium comprised of casamino acids, yeast extract, and sodium chloride. DSMZ Medium 70 Carrine Blank Thiobacillus ferrooxidans medium with ferrous sulfate Acidithiobacillus ferrooxidans medium (ferrous sulfate) http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium70.pdf 70. ACIDITHIOBACILLUS FERROOXIDANS MEDIUM (FERROUS SULFATE) KH2PO4 0.4 g MgSO4 x 7 H2O 0.4 g (NH4)2SO4 0.4 g FeSO4 x 7 H2O 33.3 g 0.1 N H2SO4 1000.0 ml Adjust pH to 1.4 with sulfuric acid. © 2007 DSMZ GmbH - All rights reserved DSM strains: 583 Acidithiobacillus ferrooxidans A minerals-salts, liquid culture medium comprised of potassium phosphate, magnesium sulfate, ammonium sulfate, ferrous sulfate and sulfuric acid. DSMZ Medium 71 DSM strains: 14366 Acidithiobacillus albertensis 584 Acidithiobacillus ferrooxidans 622 Acidithiobacillus thiooxidans 14887 Acidithiobacillus thiooxidans ATCC 19377 Carrine Blank A minerals-salts, liquid culture medium comprised of potassium phosphate, magnesium sulfate, ammonium sulfate, calcium chloride, and sodium thiosulfate. Thiobacillus ferrooxidans medium with thiosulfate Acidithiobacillus ferrooxidans medium (thiosulfate) http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium71.pdf 71. ACIDITHIOBACILLUS FERROOXIDANS MEDIUM (THIOSULFATE) KH2PO4 3.00 g MgSO4 x 7 H2O 0.50 g (NH4)2SO4 3.00 g CaCl2 x 2 H2O 0.25 g Na2S2O3 x 5 H2O 5.00 g Distilled water 1000.00 ml Prepare the medium without thiosulfate, adjust pH to 4.4 - 4.7 and autoclave at 121˚C for 15 min. Sterilize thiosulfate separately by filtration and add after autoclaving. For DSM 585 replace thiosulfate with 5.00 g/l tetrathionate (K2S4O6) added from a stock solution sterilized by filtration. For DSM 14366: Add 10.0 mg/l FeSO4 x 7 H2O. The final pH of the medium should be 4.4. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 71.1 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium71.pdf 71. ACIDITHIOBACILLUS FERROOXIDANS MEDIUM (THIOSULFATE) KH2PO4 3.00 g MgSO4 x 7 H2O 0.50 g (NH4)2SO4 3.00 g CaCl2 x 2 H2O 0.25 g Na2S2O3 x 5 H2O 5.00 g Distilled water 1000.00 ml Prepare the medium without thiosulfate, adjust pH to 4.4 - 4.7 and autoclave at 121˚C for 15 min. Sterilize thiosulfate separately by filtration and add after autoclaving. For DSM 585 replace thiosulfate with 5.00 g/l tetrathionate (K2S4O6) added from a stock solution sterilized by filtration. For DSM 14366: Add 10.0 mg/l FeSO4 x 7 H2O. The final pH of the medium should be 4.4. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 71.1 -< for DSM 585 Carrine Blank Similar to DSMZ Medium 71, except that thiosulfate is omitted and potassium tetrathionate added. DSM 585 is Acidithiobacillus ferrooxidans. Straininfo.net claims that no sequences found for this strain. Strain not in TaxBrowser. DSMZ Medium 71.2 DSMZ Medium 71.2 -< for DSM 14366 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium71.pdf 71. ACIDITHIOBACILLUS FERROOXIDANS MEDIUM (THIOSULFATE) KH2PO4 3.00 g MgSO4 x 7 H2O 0.50 g (NH4)2SO4 3.00 g CaCl2 x 2 H2O 0.25 g Na2S2O3 x 5 H2O 5.00 g Distilled water 1000.00 ml Prepare the medium without thiosulfate, adjust pH to 4.4 - 4.7 and autoclave at 121˚C for 15 min. Sterilize thiosulfate separately by filtration and add after autoclaving. For DSM 585 replace thiosulfate with 5.00 g/l tetrathionate (K2S4O6) added from a stock solution sterilized by filtration. For DSM 14366: Add 10.0 mg/l FeSO4 x 7 H2O. The final pH of the medium should be 4.4. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 14366 is Acidithiobacillus albertensis Similar to DSMZ Medium 71, except that ferrous sulfate is added. DSMZ Medium 69 DSM strains: 582 Paracoccus versutus 506 Starkeya novella DSM 506 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium69.pdf 69. THIOBACILLUS NOVELLUS MEDIUM Solution A: Na2HPO4 x 12 H2O 10.60 g KH2PO4 1.50 g NH4Cl 0.30 g Yeast extract 0.30 g Phenol red 2.00 mg Distilled water 900.00 ml Solution B: MgSO4 x 7 H2O 0.10 g Distilled water 50.00 ml Solution C: Trace element solution (see below) 5.00 ml Solution D: Na2S2O3 x 5 H2O 5.00 g Distilled water 50.00 ml Solutions A, B, C, D are sterilized separately by autoclaving and mixed when cold. For agar medium add 1.5% agar (Difco Bacto) to solution A. Adjust pH to 8.5 with sterile 0.5 N NaOH. Trace element solution (Vishniac and Santer, 1957): Na2-EDTA 50.00 g ZnSO4 x 7 H2O 22.00 g CaCl2 x 2 H2O 5.54 g MnCl2 x 4 H2O 5.06 g FeSO4 x 7 H2O 5.00 g (NH4)6Mo7O24 x 4 H2O 1.10 g CuSO4 x 5 H2O 1.57 g CoCl2 x 6 H2O 1.61 g Distilled water 1000.00 ml Adjust pH to 6.0 with KOH. © 2007 DSMZ GmbH - All rights reserved A minerals-salts, liquid culture medium containing sodium phosphate, potassium phosphate, ammonium chloride, yeast extract, phenol red, mangensium sulfate, trace elements, and sodium thiosulfate. Thiobacillus novellus medium DSMZ Medium 68 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium68.pdf 68. THIOBACILLUS NEAPOLITANUS MEDIUM KH2PO4 4.0 g K2HPO4 4.0 g MgSO4 x 7 H2O 0.8 g NH4Cl 0.4 g Trace element solution (see medium 69) 5.0 ml Na2S2O3 x 5 H2O 10.0 g Bromocresol purple (saturated aqueous solution) 0.5 ml Distilled water 1000.0 ml Adjust pH to 6.6 - 7.0. Sterilize Na2S2O3 separately in 100 ml of distilled water. For agar medium use 1.5% Difco Bacto Agar. © 2007 DSMZ GmbH - All rights reserved Thiobacillus neapolitanus medium DSM strains: 581 Halothiobacillus neapolitanus 15147 Halothiobacillus neapolitanus 16832 Halothiobacillus neapolitanus Carrine Blank A minerals-salts, liquid culture medium comprised of potassium phosphate, magnesium sulfate, ammonium chloride, trace elements, sodium thiosulfate, and bromcresol purple. DSMZ Medium 67 DSM strains: 3685 Chitinophaga arvensicola 2588 Chitinophaga pinensis DSM 2588 2589 Chitinophaga pinensis 784 Chondromyces crocatus 14714 Corallococcus coralloides 14687 Corallococcus coralloides 14688 Corallococcus exiguus 14696 Cystobacter badius 14738 Cystobacter gracilis 14753 Cystobacter gracilis 14754 Cystobacter gracilis 14755 Cystobacter gracilis 14771 Cystobacter gracilis 14756 Cystobacter miniatus 14751 Cystobacter minus 14752 Cystobacter minus 14772 Cystobacter minus 14758 Cystobacter violaceus Cb vi76 14759 Cystobacter violaceus 3656 Cytophaga 3657 Cytophaga 2063 Flavobacterium hydatis 2064 Flavobacterium johnsoniae UW101 6368 Flavobacterium pectinovorum 3660 Flavobacterium psychrophilum DSM 3660 1811 Flavobacterium saccharophilum 425 Flavobacterium sp. DSM 425 4001 Flavobacterium succinicans 4002 Flavobacterium succinicans 4003 Flavobacterium succinicans 21789 Flavobacterium swingsii DSM 21789 3661 Flavobacterium xanthum 6792 Flavobacterium johnsoniae 6793 Flexibacter flexilis DSM 6793 3098 Flexibacter 785 Herpetosiphon aurantiacus DSM 785 6205 Herpetosiphon aurantiacus 6206 Herpetosiphon aurantiacus 7119 Herpetosiphon geysericola 589 Herpetosiphon 6207 Herpetosiphon 11115 Hymenobacter chitinivorans 11117 Hymenobacter ocellatus 2044 Lysobacter antibioticus 2045 Lysobacter antibioticus 2043 Lysobacter enzymogenes 6980 Lysobacter gummosus 3655 Lysobacter 14740 Melittangium lichenicola 14675 Myxococcus stipitatus DSM 14675 14676 Myxococcus stipitatus 14700 Myxococcus stipitatus 4946 Myxococcus virescens 6796 Myxococcus xanthus 6797 Myxococcus xanthus 53668 Sandaracinus amylolyticus 2582 Sphingobacterium spiritivorum ATCC 33300 14737 Stigmatella hybrida 7029 An organic-rich, solid culture medium containing casitone, calcium chloride, yeast extract, and agar. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium67.pdf 67. CY-AGAR Casitone 3.00 g CaCl2 x 2 H2O 1.36 g Yeast extract 1.00 g Agar 15.00 g Distilled water 1000.00 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved CY-agar DSMZ Medium 65 GYM Streptomyces medium An organic-rich, solid culture medium comprised of glucose, yeast extract, malt extract, calcium carbonate, and agar. Carrine Blank DSM strains: 2579 hits in BacDive http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf 65. GYM STREPTOMYCES MEDIUM Glucose 4.0 g Yeast extract 4.0 g Malt extract 10.0 g CaCO3 2.0 g Agar 12.0 g Distilled water 1000.0 ml Adjust pH to 7.2 before adding agar. Delete CaCO3 if liquid medium is used. © 2007 DSMZ GmbH - All rights reserved dissected liver tissue An undefined organic chemical mixture, derived from dissected (physically extracted) liver tissue of an animal (Mammalia). extracted liver tissue Carrine Blank trace elements solution - Vishniac and Santer 1957 Carrine Blank A trace elements solution comprised of disodium EDTA, zinc sulfate, calcium chloride, manganese chloride, ferrous sulfate, ammonium molybdate, copper sulfate, and cobalt chloride. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium69.pdf 69. THIOBACILLUS NOVELLUS MEDIUM Solution A: Na2HPO4 x 12 H2O 10.60 g KH2PO4 1.50 g NH4Cl 0.30 g Yeast extract 0.30 g Phenol red 2.00 mg Distilled water 900.00 ml Solution B: MgSO4 x 7 H2O 0.10 g Distilled water 50.00 ml Solution C: Trace element solution (see below) 5.00 ml Solution D: Na2S2O3 x 5 H2O 5.00 g Distilled water 50.00 ml Solutions A, B, C, D are sterilized separately by autoclaving and mixed when cold. For agar medium add 1.5% agar (Difco Bacto) to solution A. Adjust pH to 8.5 with sterile 0.5 N NaOH. Trace element solution (Vishniac and Santer, 1957): Na2-EDTA 50.00 g ZnSO4 x 7 H2O 22.00 g CaCl2 x 2 H2O 5.54 g MnCl2 x 4 H2O 5.06 g FeSO4 x 7 H2O 5.00 g (NH4)6Mo7O24 x 4 H2O 1.10 g CuSO4 x 5 H2O 1.57 g CoCl2 x 6 H2O 1.61 g Distilled water 1000.00 ml Adjust pH to 6.0 with KOH. © 2007 DSMZ GmbH - All rights reserved Phytone peptone A papaic digest of soybean (Hordeum vulgare) meal, used for the culturing of microorganisms. Phytone™ Peptone is a trademark of BBL/Difco Labs. Carrine Blank papaic digest of soybean meal From BD Bionutrients Technical Manual (3rd edition revised): "Many of these media utilize peptones of known derivation, such as Trypticase™ Peptone, a pancreatic digest of casein, and Phytone™ Peptone, a papaic digest of soybean meal." DSMZ Medium 78.1 DSM 5566 is Clostridium novyi B str. ATCC 27606 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium78.pdf 78. CHOPPED MEAT MEDIUM Ground beef (fat free) 500.0 g Distilled water 1000.0 ml NaOH 1 N 25.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: Casitone 30.0 g Yeast extract 5.0 g K2HPO4 5.0 g Resazurin 1.0 mg Boil, cool under nitrogen atmosphere, add 0.5 g/l cysteine and adjust pH to 7.0. To make medium anoxic boil it, cool under nitrogen atmosphere, add 0.5 g/l cysteine hydrochloride and adjust pH to 7.0. Dispense anoxically 7 ml medium into Hungate tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121˚C for 30 min. For agar slants use 15 g agar per 1000.0 ml medium. For DSM 5566 add to the autoclaved medium 1 g/l NaHCO3 from a sterile, anoxic stock solution (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture. Adjust final pH of medium to 7.2. In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving: Haemin solution (see below) 10.00 ml Vitamin K1 or Vitamin K3 solution (see below) 0.20 ml Haemin solution: Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. Vitamin K1 solution: Dissolve 0.1 ml of vitamin K1/K3 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. Vitamin K3 solution: Dissolve 5 mg/ml of vitamin K3 in 10 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. © 2011 DSMZ GmbH - All rights reserved Similar to DSM Medium 78, except that sodium bicarbonate is added. Prepared under an atmosphere of dinitrogen and carbon dioxide. The pH is increased to 7.2. Carrine Blank DSMZ Medium 78.1 -< for DSM 5566 vitamin solution for DSMZ Medium 78a A vitamin solution containing vitamin B12 (cobalamin), pantothenic acid, riboflavin, pyridoxamine hydrochloride, biotin, folic acid, nicotinic acid, nicotine amide (nicotinamide), alpha-lipoic acid (lipoic acid), p-aminobenzoic acid (4-aminobenzoic acid), and thiamine hydrochloride. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium78a.pdf 78a. CHOPPED MEAT MEDIUM FOR TREPONEMA SP. To the ready medium 78, add 50 ml/l amino acid solution and 5 ml/l vitamin solution. These solutions must not be prepared under anaerobic conditions. Amino acid solution (filter sterilized): L-Histidine 0.6 g L-Serine 0.5 g L-Glutamine 0.7 g Distilled water 50.0 ml Vitamin solution: Vitamin B12 50 mg Pantothenic acid 50 mg Riboflavin 50 mg Pyridoxamine-HCl 10 mg Biotin 20 mg Folic acid 20 mg Nicotinic acid 25 mg Nicotine amide 25 mg α-lipoic acid 50 mg p-aminobenzoic acid 50 mg Thiamine-HCl x 2 H2O 50 mg Distilled water 1000 ml Stir for some hours, filter sterilize the solution. © 2010 DSMZ GmbH - All rights reserved Carrine Blank synthetic sea water for DSMZ Medium 79 An artificial (synthetic) seawater comprised of sodium chloride, magnesium chloride, sodium sulfate, calcium chloride, potassium chloride, potassium bromide, boric acid, sodium silicate, strontium chloride, sodium fluoride, ammonium nitrate, and ferric phosphate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium79.pdf 79. LEUCOTHRIX MEDIUM Tryptone 10.00 g Synthetic sea water 1000.00 ml Synthetic sea water: NaCl 24.00 g MgCl2 x 6 H2O 11.00 g Na2SO4 4.00 g CaCl2 x 6 H2O 2.00 g KCl 0.70 g KBr 0.10 g H3BO3 0.03 g NaSiO3 x 9 H2O 5.00 mg SrCl2 x 6 H2O 0.04 g NaF 3.00 mg NH4NO3 2.00 mg Fe3PO4 x 4 H2O 1.00 mg Distilled water 1000.00 ml Adjust pH to 7.8. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 302 Nutrient broth with 10% horse serum Similar to DSMZ Medium 1, except horse serum is added. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium302.pdf 302. NUTRIENT BROTH WITH 10% HORSE SERUM To autoclaved medium 1 add 100 ml/l of sterile horse serum. © 2007 DSMZ GmbH - All rights reserved Carrine Blank amino acid solution for DSMZ Medium 78a A defined organic solution containing the following amino acids: L-histidine, L-serine, and L-glutamine. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium78a.pdf 78a. CHOPPED MEAT MEDIUM FOR TREPONEMA SP. To the ready medium 78, add 50 ml/l amino acid solution and 5 ml/l vitamin solution. These solutions must not be prepared under anaerobic conditions. Amino acid solution (filter sterilized): L-Histidine 0.6 g L-Serine 0.5 g L-Glutamine 0.7 g Distilled water 50.0 ml Vitamin solution: Vitamin B12 50 mg Pantothenic acid 50 mg Riboflavin 50 mg Pyridoxamine-HCl 10 mg Biotin 20 mg Folic acid 20 mg Nicotinic acid 25 mg Nicotine amide 25 mg α-lipoic acid 50 mg p-aminobenzoic acid 50 mg Thiamine-HCl x 2 H2O 50 mg Distilled water 1000 ml Stir for some hours, filter sterilize the solution. © 2010 DSMZ GmbH - All rights reserved filtered garden soil extract Carrine Blank A soil extract made from garden soil. Prepared by seiving garden soil through a coarse sieve, adding distilled water, and autoclaving. standard vitamin solution for DSMZ Medium 81 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium81.pdf 81. MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) Solution A: KH2PO4 2.300 g Na2HPO4 x 2 H2O 2.900 g Distilled water 50.000 ml Solution B: NH4Cl 1.000 g MgSO4 x 7 H2O 0.500 g CaCl2 x 2 H2O 0.010 g MnCl2 x 4 H2O 0.005 g NaVO3 x H2O 0.005 g Trace element sol. SL-6 (see medium 27) 5.000 ml Distilled water 915.000 ml Agar (if necessary) 20.000 g Solution C: Ferric ammonium citrate 0.050 g Distilled water 20.000 ml Solutions A, B, C are autoclaved separately for 15 min at 121˚C, cooled down to 50˚C and then mixed aseptically with 5.0 ml filter-sterilized standard vitamin solution (see below) and 10.0 ml filter-sterilized 5% NaHCO3 (pH 7-8).The final pH of this medium should be 6.8 without adjustment. For chemolithotrophic growth incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 60% H2 and 28% N2. For heterotrophic growth supplement the mineral medium with an appropriate carbon source (0.2% carbohydrate or 0.1% organic acid). For growth on nitrogen-free medium, omit NH4Cl and incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 10% H2 and 78% N2 or heterotrophically under 2% (v/v) O2 and 98% N2. For more details see Ref. 1515 and Ref. 3363. For DSM 21436 adjust pH to 5.0 and use a gas atmosphere of 10% O2, 10% CO2, 40% H2 and 40% N2 with an overpressure of 2 bar. Standard vitamin solution: Riboflavin 10.000 mg Thiamine-HCl x 2 H2O 50.000 mg Nicotinic acid 50.000 mg Pyridoxine-HCl 50.000 mg Ca-pantothenate 50.000 mg Biotin 0.100 mg Folic acid 0.200 mg Vitamin B12 1.000 mg Distilled water 100.000 © 2011 DSMZ GmbH - All rights reserved A vitamin solution comprised of riboflavin, thiamine hydrochloride, nicotinic acid, pyridoxine hydrochloride, calcium pantothenate, biotin, folic acid, and vitamin B12 (cobalamin). rolled oats Wikipedia: Rolled oats Rolled oats are traditionally oat groats that have been de-husked, steamed and then rolled into flat flakes under heavy rollers before being stabilized by being lightly toasted. The oat, like the other cereals, has a hard, inedible outer husk that must be removed before the grain can be eaten. After the outer husk (or chaff) has been removed from the still bran-covered oat grains, the remainder is called oat groats. Oat groats are a whole grain that can be used as a breakfast cereal; various forms of oatmeal and rolled oats, and pinhead oats are cooked to make porridge.[1] Steel-cut oats (pinhead oatmeal) are oat groats that have been chopped into smaller pieces before any steaming and thus retain bits of the bran layer. Since the bran layer, though nutritious, makes the grains tough to chew and contains an enzyme that can cause the oats to go rancid, raw oat groats are often further steam-treated to soften them for a quicker cooking time (modern "quick oats") and to denature the enzymes for a longer shelf life. Undefined organic chemical mixture comprised of de-husked oat (Avena sativa) seed that has been crushed by rolling under a heavy roller and toasted. oat flakes Carrine Blank vitamin solution CA http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium87a.pdf 87a. CHLOROFLEXUS AGGREGANS MEDIUM To medium 87 add 1.0 ml/litre sterile vitamin solution CA. Vitamin solution CA Distilled water 100.0 ml Nicotinic acid 100.0 mg Thiamine-HCl x 2 H2O 100.0 mg Biotin 5.0 mg p-Aminobenzoic acid 50.0 mg Vitamin B12 1.0 mg Ca-pantothenate 50.0 mg Pyridoxine-HCl 50.0 mg Folic acid 50.0 mg Na3-EDTA 200.0 mg Adjust pH to 7.5. The solution is filter-sterilized. © 2007 DSMZ GmbH - All rights reserved Carrine Blank A vitamin solution comprised of nicotinic acid, thiamine hydrochloride, biotin, p-aminobenzoic acid (4-aminobenzoic acid), vitamin B12 (cobalamin), calcium pantothenate, pyridoxine hydrochloride, folic acid, and trisodium EDTA. DSZM Medium 131 DSM strains: 1053 Methanothermobacter thermautotrophicus str. Delta H 6216 Methanoculleus bourgensis A minerals-salts, liquid culture medium comprised of sodium carbonate, ammonium sulfate, sodium chloride, potassium phosphate, magnesium sulfate, calcium chloride, ferrous sulfate, resazurin, vitamin solution, trace elements solution, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen and carbon dioxide. Carrine Blank (note this media recipe is not in the current list of media recipes at the DSMZ). From: Handbook of Microbiological Media, 3rd edition. By Ronald M. Atlas. pp. 1056. Composition per liter: sodium carbonate, 4.0g ammonium sulfate, 1.5g sodium chloride, 0.6g potassium dihydrogen phosphate, 0.3g potassium dibasic phosphate, 0.15g magnesium sulfate heptahydrate, 0.12g calcium chloride dihyrate, 0.08g iron sulfate heptahydrate, 4.0mg resazurin,1.0mg vitamin solution, 10.0 mL trace elements solution, 10.0 mL L-cysteine solution, 10.0 mL sodium sulfide nonahydrate solution, 10.0 mL pH 7.2 ± 0.2 at 25˚C. Vitamin solution Composition per liter: pyridoxine hydrochloride, 10.0mg thiamine hydrochloride dihydrate, 5.0 mg riboflavine, 5.0 mg nicotinic acid, 5.0 mg calcium pantothenate, 5.0 mg biotin, 2.0 mg folic acid, 2.0 mg p-aminobenzoic acid, 1.0 mg vitamin B12, 0.01 mg Preparation of Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% dihydrogen and 20% carbon dioxide. Filter sterilize. Trace elements solution Composition per liter: magnesium sulfate heptahydrate, 6.2g sodium chloride, 1.0g disodium EDTA, 0.64g manganese sulfate tetrahydrate, 0.55g zinc sulfate heptahydrate, 0.18g cobalt chloride hexahydrate, 0.17g calcium chloride dihydrate, 0.13g iron sulfate heptahydrate, 0.1g copper sulfate, 0.05g nickel chloride hexahydrate, 0.025g potassium aluminum sulfate dodecadydrate, 0.018g sodium molybdate tetrahydrate, 0.011g boric acid, 0.01g Preparation of Trace Elements Solution: Add Na2-EDTA to 500.0 mL distilled/deionized water. Mix thoroughly. Add other components and bring volume to 1.0 L with distilled/deionized water. Mix thoroughly. Sparge with 80% dihydrogen and 20% carbon dioxide. Autoclave for 15 min at 15 psi pressure-121˚C. Sodium sulfide solution Composition per 10.0 mL: sodium sulfide nonahydrate, 1.5g Preparation of Sodium sulfide solution: add sodium sulfide to distilled/deionized water and bring volume to 10.0 mL. Sparge with dinitrogen. Autoclave for 15 min at 15 psi pressure-121˚C. Cool to 25˚C. Store anaerobically. L-Cystene Solution Composition per 10.0 m:" L-cysteine hydrochloride monohydrate, 1.5g Preparation of L-Cysteine solution: Add L-cysteine-HCL-H2O to distilled/deionized water and bring volume to 10.0 mL. Mix thoroughly. Sparge with dinitrogen. Autoclave for 15 min at 15 psi pressure-121˚C> Preparation of Medium: prepare and dispense medium under an oxygen-free 80% dihydrogen 20% carbon dioxide gas mixture. Add components, except vitamin solution, L-cystene solution, and sodium sulfide solution to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with H2/CO2. Autocalve for 15 min at 15 psi pressure-121˚C. Cool to 25˚C while sparging with H2/CO2. Aseptically and anaerobically add 10.0 mL vitamin solution, 10.0 mL of sterile L-cystene soltuion, and 10.0 mL of sterile sodium sulfide solution. Mix thoroughly. Aseptically and anaerobially distribute into sterile tubes or flasks. Alternately the medium can be distributed to tubes under anaerobic conditions and autoclaved in tubes prior to addition of substrate solution, vitamin solution, and sodium sulfide solution. Appropriate ammounts of these solutions can then be..... vitamin solution for DSMZ Medium 131 Carrine Blank A vitamin solution comprised of pyridoxine hydrochloride, thiamine hydrochloride, riboflavin, nicotinic acid, calcium pantothenate, biotin, folic acid, p-aminobenzoic acid(4-aminobenzoic acid), and vitamin B12 (cobalamin). Prepared under an atmosphere of dihydrogen and carbon dioxide. (note this media recipe is not in the current list of media recipes at the DSMZ). From: Handbook of Microbiological Media, 3rd edition. By Ronald M. Atlas. pp. 1056. Composition per liter: sodium carbonate, 4.0g ammonium sulfate, 1.5g sodium chloride, 0.6g potassium dihydrogen phosphate, 0.3g potassium dibasic phosphate, 0.15g magnesium sulfate heptahydrate, 0.12g calcium chloride dihyrate, 0.08g iron sulfate heptahydrate, 4.0mg resazurin,1.0mg vitamin solution, 10.0 mL trace elements solution, 10.0 mL L-cysteine solution, 10.0 mL sodium sulfide nonahydrate solution, 10.0 mL pH 7.2 ± 0.2 at 25˚C. Vitamin solution Composition per liter: pyridoxine hydrochloride, 10.0mg thiamine hydrochloride dihydrate, 5.0 mg riboflavine, 5.0 mg nicotinic acid, 5.0 mg calcium pantothenate, 5.0 mg biotin, 2.0 mg folic acid, 2.0 mg p-aminobenzoic acid, 1.0 mg vitamin B12, 0.01 mg Preparation of Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% dihydrogen and 20% carbon dioxide. Filter sterilize. Trace elements solution Composition per liter: magnesium sulfate heptahydrate, 6.2g sodium chloride, 1.0g disodium EDTA, 0.64g manganese sulfate tetrahydrate, 0.55g zinc sulfate heptahydrate, 0.18g cobalt chloride hexahydrate, 0.17g calcium chloride dihydrate, 0.13g iron sulfate heptahydrate, 0.1g copper sulfate, 0.05g nickel chloride hexahydrate, 0.025g potassium aluminum sulfate dodecadydrate, 0.018g sodium molybdate tetrahydrate, 0.011g boric acid, 0.01g Preparation of Trace Elements Solution: Add Na2-EDTA to 500.0 mL distilled/deionized water. Mix thoroughly. Add other components and bring volume to 1.0 L with distilled/deionized water. Mix thoroughly. Sparge with 80% dihydrogen and 20% carbon dioxide. Autoclave for 15 min at 15 psi pressure-121˚C. Sodium sulfide solution Composition per 10.0 mL: sodium sulfide nonahydrate, 1.5g Preparation of Sodium sulfide solution: add sodium sulfide to distilled/deionized water and bring volume to 10.0 mL. Sparge with dinitrogen. Autoclave for 15 min at 15 psi pressure-121˚C. Cool to 25˚C. Store anaerobically. L-Cystene Solution Composition per 10.0 m:" L-cysteine hydrochloride monohydrate, 1.5g Preparation of L-Cysteine solution: Add L-cysteine-HCL-H2O to distilled/deionized water and bring volume to 10.0 mL. Mix thoroughly. Sparge with dinitrogen. Autoclave for 15 min at 15 psi pressure-121˚C> Preparation of Medium: prepare and dispense medium under an oxygen-free 80% dihydrogen 20% carbon dioxide gas mixture. Add components, except vitamin solution, L-cystene solution, and sodium sulfide solution to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with H2/CO2. Autocalve for 15 min at 15 psi pressure-121˚C. Cool to 25˚C while sparging with H2/CO2. Aseptically and anaerobically add 10.0 mL vitamin solution, 10.0 mL of sterile L-cystene soltuion, and 10.0 mL of sterile sodium sulfide solution. Mix thoroughly. Aseptically and anaerobially distribute into sterile tubes or flasks. Alternately the medium can be distributed to tubes under anaerobic conditions and autoclaved in tubes prior to addition of substrate solution, vitamin solution, and sodium sulfide solution. Appropriate ammounts of these solutions can then be..... trace elements solution for DSMZ Medium 131 (note this media recipe is not in the current list of media recipes at the DSMZ). From: Handbook of Microbiological Media, 3rd edition. By Ronald M. Atlas. pp. 1056. Composition per liter: sodium carbonate, 4.0g ammonium sulfate, 1.5g sodium chloride, 0.6g potassium dihydrogen phosphate, 0.3g potassium dibasic phosphate, 0.15g magnesium sulfate heptahydrate, 0.12g calcium chloride dihyrate, 0.08g iron sulfate heptahydrate, 4.0mg resazurin,1.0mg vitamin solution, 10.0 mL trace elements solution, 10.0 mL L-cysteine solution, 10.0 mL sodium sulfide nonahydrate solution, 10.0 mL pH 7.2 ± 0.2 at 25˚C. Vitamin solution Composition per liter: pyridoxine hydrochloride, 10.0mg thiamine hydrochloride dihydrate, 5.0 mg riboflavine, 5.0 mg nicotinic acid, 5.0 mg calcium pantothenate, 5.0 mg biotin, 2.0 mg folic acid, 2.0 mg p-aminobenzoic acid, 1.0 mg vitamin B12, 0.01 mg Preparation of Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% dihydrogen and 20% carbon dioxide. Filter sterilize. Trace elements solution Composition per liter: magnesium sulfate heptahydrate, 6.2g sodium chloride, 1.0g disodium EDTA, 0.64g manganese sulfate tetrahydrate, 0.55g zinc sulfate heptahydrate, 0.18g cobalt chloride hexahydrate, 0.17g calcium chloride dihydrate, 0.13g iron sulfate heptahydrate, 0.1g copper sulfate, 0.05g nickel chloride hexahydrate, 0.025g potassium aluminum sulfate dodecadydrate, 0.018g sodium molybdate tetrahydrate, 0.011g boric acid, 0.01g Preparation of Trace Elements Solution: Add Na2-EDTA to 500.0 mL distilled/deionized water. Mix thoroughly. Add other components and bring volume to 1.0 L with distilled/deionized water. Mix thoroughly. Sparge with 80% dihydrogen and 20% carbon dioxide. Autoclave for 15 min at 15 psi pressure-121˚C. Sodium sulfide solution Composition per 10.0 mL: sodium sulfide nonahydrate, 1.5g Preparation of Sodium sulfide solution: add sodium sulfide to distilled/deionized water and bring volume to 10.0 mL. Sparge with dinitrogen. Autoclave for 15 min at 15 psi pressure-121˚C. Cool to 25˚C. Store anaerobically. L-Cystene Solution Composition per 10.0 m:" L-cysteine hydrochloride monohydrate, 1.5g Preparation of L-Cysteine solution: Add L-cysteine-HCL-H2O to distilled/deionized water and bring volume to 10.0 mL. Mix thoroughly. Sparge with dinitrogen. Autoclave for 15 min at 15 psi pressure-121˚C> Preparation of Medium: prepare and dispense medium under an oxygen-free 80% dihydrogen 20% carbon dioxide gas mixture. Add components, except vitamin solution, L-cystene solution, and sodium sulfide solution to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with H2/CO2. Autocalve for 15 min at 15 psi pressure-121˚C. Cool to 25˚C while sparging with H2/CO2. Aseptically and anaerobically add 10.0 mL vitamin solution, 10.0 mL of sterile L-cystene soltuion, and 10.0 mL of sterile sodium sulfide solution. Mix thoroughly. Aseptically and anaerobially distribute into sterile tubes or flasks. Alternately the medium can be distributed to tubes under anaerobic conditions and autoclaved in tubes prior to addition of substrate solution, vitamin solution, and sodium sulfide solution. Appropriate ammounts of these solutions can then be..... Carrine Blank A trace elements solution comprised of magnesium sulfate, sodium chloride, disodium EDTA, manganese sulfate, zinc sulfate, cobalt chloride, calcium chloride, ferrous sulfate, copper sulfate, nickel chloride, potassium aluminum sulfate, sodium molybdate, and boric acid. Prepared under an atmosphere of dihydrogen and carbon dioxide. DSMZ Medium 104 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104.pdf 104. PYG MEDIUM (modified) Trypticase peptone 5.00 g Peptone 5.00 g Yeast extract 10.00 g Beef extract 5.00 g Glucose 5.00 g K2HPO4 2.00 g Tween 80 1.00 ml Cysteine-HCl x H2O 0.50 g Resazurin 1.00 mg Salt solution (see below) 40.00 ml Distilled water 950.00 ml Haemin solution (see below) 10.00 ml Vitamin K1 solution (see below) 0.20 ml The vitamin K1, haemin solution and the cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and autoclave. Salt solution: CaCl2 x 2 H2O 0.25 g MgSO4 x 7 H2O 0.50 g K2HPO4 1.00 g KH2PO4 1.00 g NaHCO3 10.00 g NaCl 2.00 g Distilled water 1000.00 ml Haemin solution: Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water. Store refrigerated. Vitamin K1 solution: Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. © 2009 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of trypticase peptone, peptone, yeast extract, beef extract, glucose, potassium phosphate, tween 80 (polysorbate 80), cysteine hydrochloride, resazurin, salt solution, hemin solution, and vitamin K solution. Prepared under a atmosphere of carbon dioxide. Carrine Blank PYG Medium (modified) DSM strains: DSMZ Medium 470 Pseudomonas halophila medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium470.pdf 470. PSEUDOMONAS HALOPHILA MEDIUM Solution A: NaCl 46.8 g MgSO4 x 7 H2O 39.4 g NH4Cl 1.0 g Glycerol 5.0 g Trace element sol. SL-10 (see medium 320) 1.0 ml Vitamin solution (see medium 131) 10.0 ml Distilled water 890.0 ml Solution B: KH2PO4 1.0 g Distilled water 100.0 ml Vitamin solution is filter-sterilized and added after autoclaving. Solution A and B are autoclaved separately and combined after cooling. Adjust pH to 7.0 with 6 M NaOH or HCl. © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of sodium chloride, magnesium sulfate, ammonium chloride, glycerol, trace elements, vitamins, and potassium phosphate. DSM strains: DSMZ Medium 851 Carrine Blank DSM strains: 12906 Tessaracoccus bendigoensis DSM 12906 12890 Tetrasphaera australiensis 13193 Tetrasphaera australiensis 13192 Tetrasphaera japonica T1-X7 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium851.pdf 851. GLUCOSE SULFIDE MEDIUM Glucose 0.15 g Yeast extract 1.00 g (NH4)2SO4 0.50 g CaCO3 0.10 g Ca(NO3)2 0.10 g KCl 0.05 g K2HPO4 0.05 g MgSO4 x 7 H2O 0.05 g Na2S x 9 H2O 0.20 g Vitamin solution (see medium 131) 10.00 ml Distilled water 990.00 ml Adjust pH to 7.3. © 2011 DSMZ GmbH - All rights reserved glucose sulfide medium An organic-rich, liquid culture medium comprised of glucose, yeast extract, ammonium sulfate, calcium carbonate, calcium nitrate, potassium chloride, potassium phosphate, magnesium sulfate, sodium sulfide, and vitamins. DSMZ Medium 11a Similar to DSMZ Medium 11, except a vitamin solution is added. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium11a.pdf 11a. MODIFIED MEDIUM 11 Prepare medium 11 with additional vitamin solution (see medium 131). © 2007 DSMZ GmbH - All rights reserved modified medium 11 DSM strains: DSMZ Medium 1131 A minerals salts, liquid culture medium comprised of artificial sea water, vitamins, sodium nitrate, sodium bicarbonate, and elemental sulfur. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1131.pdf 1131. HYDROGENIVIRGA OKINAWENSIS MEDIUM Modified MJ synthetic sea water (see below) 1000.0 ml Trace vitamins (see next page) 10.0 ml NaNO3 1.0 g NaHCO3 1.0 g Sulfur (powder) 3.0 g Mix ingredients except NaHCO3 and vitamin solution, adjust pH to 7.0 with NaOH, and autoclave under a N2 atmosphere. Filter-sterilize 8% NaHCO3 solution and add to the medium. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a stream of a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Steam medium for 3 hr on each of 3 successive days. Prior to inoculation, add trace vitamins (sterile filtrated and stocked under a N2 atmosphere) to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v). Modified MJ synthetic sea water: NaCl 30.00 g K2HPO4 0.14 g CaCl2 x 2 H2O 0.50 g NH4Cl 0.50 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.18 g KCl 0.33 g FeSO4 x 7 H2O 0.01 g NiCl2 x 6 H2O 1.00 mg Na2SeO3 x 5 H2O 1.00 mg Trace element solution (see below) 10.00 ml Distilled water 1000.00 ml Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x X H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.10 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.10 g CuSO4 x 5 H2O 0.01 g AlK(SO4)2 0.01 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml © 2012 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank Hydrogenivirga okinawensis medium modified MJ synthetic sea water An artificial seawater comprised of sodium chloride, potassium phosphate, calcium chloride, ammonium chloride, magnesium suflate, magnesium chloride, potassium chloride, ferrous sulfate, nickel chloride, sodium selenite, and trace elements. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1131.pdf 1131. HYDROGENIVIRGA OKINAWENSIS MEDIUM Modified MJ synthetic sea water (see below) 1000.0 ml Trace vitamins (see next page) 10.0 ml NaNO3 1.0 g NaHCO3 1.0 g Sulfur (powder) 3.0 g Mix ingredients except NaHCO3 and vitamin solution, adjust pH to 7.0 with NaOH, and autoclave under a N2 atmosphere. Filter-sterilize 8% NaHCO3 solution and add to the medium. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a stream of a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Steam medium for 3 hr on each of 3 successive days. Prior to inoculation, add trace vitamins (sterile filtrated and stocked under a N2 atmosphere) to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v). Modified MJ synthetic sea water: NaCl 30.00 g K2HPO4 0.14 g CaCl2 x 2 H2O 0.50 g NH4Cl 0.50 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.18 g KCl 0.33 g FeSO4 x 7 H2O 0.01 g NiCl2 x 6 H2O 1.00 mg Na2SeO3 x 5 H2O 1.00 mg Trace element solution (see below) 10.00 ml Distilled water 1000.00 ml Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x X H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.10 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.10 g CuSO4 x 5 H2O 0.01 g AlK(SO4)2 0.01 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml © 2012 DSMZ GmbH - All rights reserved Carrine Blank trace elements solution for DSMZ Medium 1131 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1131.pdf 1131. HYDROGENIVIRGA OKINAWENSIS MEDIUM Modified MJ synthetic sea water (see below) 1000.0 ml Trace vitamins (see next page) 10.0 ml NaNO3 1.0 g NaHCO3 1.0 g Sulfur (powder) 3.0 g Mix ingredients except NaHCO3 and vitamin solution, adjust pH to 7.0 with NaOH, and autoclave under a N2 atmosphere. Filter-sterilize 8% NaHCO3 solution and add to the medium. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a stream of a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Steam medium for 3 hr on each of 3 successive days. Prior to inoculation, add trace vitamins (sterile filtrated and stocked under a N2 atmosphere) to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v). Modified MJ synthetic sea water: NaCl 30.00 g K2HPO4 0.14 g CaCl2 x 2 H2O 0.50 g NH4Cl 0.50 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.18 g KCl 0.33 g FeSO4 x 7 H2O 0.01 g NiCl2 x 6 H2O 1.00 mg Na2SeO3 x 5 H2O 1.00 mg Trace element solution (see below) 10.00 ml Distilled water 1000.00 ml Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x X H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.10 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.10 g CuSO4 x 5 H2O 0.01 g AlK(SO4)2 0.01 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml © 2012 DSMZ GmbH - All rights reserved A trace elements solution comprised of nitrilotriacetic acid, magnesium sulfate, manganese sulfate, sodium chloride, ferrous sulfate, cobalt sulfate, calcium chloride, zinc sulfate, copper sulfate, potassium aluminum sulfate, boric acid, and sodium molybdate. vitamin solution for DSMZ Medium 1131 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1131.pdf 1131. HYDROGENIVIRGA OKINAWENSIS MEDIUM Modified MJ synthetic sea water (see below) 1000.0 ml Trace vitamins (see next page) 10.0 ml NaNO3 1.0 g NaHCO3 1.0 g Sulfur (powder) 3.0 g Mix ingredients except NaHCO3 and vitamin solution, adjust pH to 7.0 with NaOH, and autoclave under a N2 atmosphere. Filter-sterilize 8% NaHCO3 solution and add to the medium. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a stream of a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Steam medium for 3 hr on each of 3 successive days. Prior to inoculation, add trace vitamins (sterile filtrated and stocked under a N2 atmosphere) to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v). Modified MJ synthetic sea water: NaCl 30.00 g K2HPO4 0.14 g CaCl2 x 2 H2O 0.50 g NH4Cl 0.50 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.18 g KCl 0.33 g FeSO4 x 7 H2O 0.01 g NiCl2 x 6 H2O 1.00 mg Na2SeO3 x 5 H2O 1.00 mg Trace element solution (see below) 10.00 ml Distilled water 1000.00 ml Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x X H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.10 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.10 g CuSO4 x 5 H2O 0.01 g AlK(SO4)2 0.01 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml © 2012 DSMZ GmbH - All rights reserved A vitamin solution comprised of biotin, folic acid, pyridoxine hydrochloride, thiamine hydrochloride, riboflavin, nicotinic acid, calcium pantothenate, vitamin B12 (cobalamin), p-aminobenzoic acid (4-aminobenzoic acid), and lipoic acid. DSMZ Medium 111 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium111.pdf 111. BEIJERINCKIA MEDIUM Glucose 10.0 g K2HPO4 0.8 g KH2PO4 0.2 g MgSO4 x 7 H2O 0.1 g FeSO4 x 7 H2O 20.0 mg MnSO4 x 6 H2O 2.0 mg ZnSO4 x 6 H2O 5.0 mg CuSO4 x 6 H2O 4.0 mg Na2MoO4 x 2 H2O 5.0 mg Agar 15.0 g Distilled water 950.0 ml Adjust pH to 6.5. Sterilize glucose separately (10 g in 50 ml H2O) and mix after cooling. © 2007 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, solid culture medium comprised of glucose, potassium phosphate, magnesium sulfate, ferrous sulfate, manganese sulfate, zinc sulfate, copper sulfate, sodium molybdate, and agar. Beijerinckia medium Carrine Blank DSMZ Medium 110a DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium110a.pdf 110a. CMC MEDIUM (N2/CO2) Ground beef (fat free) 500.0 g Distilled water 1000.0 ml NaOH 1 N 25.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: Casitone 30.0 g Yeast extract 5.0 g K2HPO4 5.0 g Resazurin 1.0 mg D-Glucose 4.0 g Cellobiose 1.0 g Maltose 1.0 g Starch (soluble) 1.0 g To make medium anoxic boil it, cool under 80% N2 + 20% CO2 gas atmosphere, add 0.5 g/l L-cysteine-HCl x H2O and dispense under same gas atmosphere into Hungate-type tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121˚C for 20 min. After autoclaving adjust pH of medium to pH 7 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 + 20% CO2 gas atmosphere. © 2014 DSMZ GmbH - All rights reserved An organic-rich liquic culture medium comprised of lean ground beef hydrolysate. To this, casitone, yeast extract, potassium phosphate, resazurin, D-glucose, cellobiose, maltose, soluble starch, and cysteine hydrochloride are added. Prepared under an atmosphere of dinitrogen and carbon dioxide. CMC medium (N2/CO2) CMC medium Carrine Blank DSMZ Medium 110 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium110.pdf 110. CHOPPED MEAT MEDIUM WITH CARBOHYDRATES Ground beef (fat free) 500.0 g Distilled water 1000.0 ml NaOH 1 N 25.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: Casitone 30.0 g Yeast extract 5.0 g K2HPO4 5.0 g Resazurin 1.0 mg Glucose 4.0 g Cellobiose 1.0 g Maltose 1.0 g Starch (soluble) 1.0 g To make medium anoxic boil it, cool under nitrogen atmosphere, add 0.5 g/l cysteine hydrochloride and adjust pH to 7.0. Dispense anoxically 7 ml medium into Hungate tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121˚C for 30 min. For agar slants use 15 g agar per 1000.0 ml medium. In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving: Haemin solution (see below) 10.00 ml Vitamin K1 or Vitamin K3 solution (see below) 0.20 ml Haemin solution: Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. Vitamin K1 solution: Dissolve 0.1 ml of vitamin K1/K3 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. Vitamin K3 solution: Dissolve 5 mg/ml of vitamin K3 in 10 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. © 2010 DSMZ GmbH - All rights reserved An organic-rich liquic culture medium comprised of lean ground beef hydrolysate. To this, casitone, yeast extract, potassium phosphate, resazurin, glucose, cellobiose, maltose, soluble starch, and cysteine hydrochloride are added. Prepared under an atmosphere of dinitrogen. chopped meat medium with carbohydrates DSM strains: Carrine Blank DSMZ Medium 78 DSM strains: 19147 Alistipes onderdonkii WAL 8169 = DSM 19147 19121 Alistipes shahii WAL 8301 2951 Anaerococcus tetradius ATCC 35098 2635 Asaccharospora irregularis DSM 2635 15896 Bacteroides acidifaciens JCM 10556 19024 Bacteroides caccae ATCC 43185 14838 Bacteroides cellulosilyticus DSM 14838 22519 Bacteroides clarus YIT 12056 20705 [Bacteroides] coagulans 26883 Bacteroides faecichinchillae JCM 17102 24798 Bacteroides faecis MAJ27 17565 Bacteroides finegoldii DSM 17565 22534 Bacteroides fluxus YIT 12057 1396 Bacteroides fragilis 2151 Bacteroides fragilis NCTC 9343 22535 Bacteroides oleiciplenus YIT 12058 1896 Bacteroides ovatus ATCC 8483 21004 Bacteroides paurosaccharolyticus JCM 15092 19346 Bacteroides propionicifaciens 19291 Bacteroides propionicifaciens DSM 19291 = JCM 14649 19673 Bacteroides pyogenes DSM 20611 = JCM 6294 20611 Bacteroides pyogenes 26882 Bacteroides rodentium JCM 16496 18765 Bacteroides salyersiae WAL 10018 = DSM 18765 = JCM 12988 19555 Bacteroides stercoris ATCC 43183 2079 Bacteroides thetaiotaomicron VPI-5482 2255 Bacteroides thetaiotaomicron 6597 Bacteroides uniformis ATCC 8492 1447 Bacteroides vulgatus ATCC 8482 3289 Bacteroides vulgatus 18836 Bacteroides xylanisolvens XB1A 21032 Barnesiella intestinihominis YIT 11860 18177 Barnesiella viscericola DSM 18177 2950 Blautia producta ATCC 27340 = DSM 2950 3507 Blautia producta 19528 Campylobacter gracilis 21671 Campylobacter hominis 21682 Campylobacter mucosalis 19458 Campylobacter showae 2779 Centipeda periodontii 23941 Cetobacterium somerae ATCC BAA-474 630 [Clostridium] bifermentans 631 [Clostridium] bifermentans 666 Clostridium cochlearium 1285 Clostridium cochlearium 2153 Clostridium cochlearium 6011 [Clostridium] colinum 27543 Peptoclostridium difficile 630 27544 Peptoclostridium difficile 28645 Peptoclostridium difficile 630 12750 Clostridium drakei 2631 Clostridium fallax DSM 2631 5565 Clostridium haemolyticum 627 Hathewaya histolytica 2158 Hathewaya histolytica 1286 [Clostridium] innocuum 5566 Clostridium novyi B str. ATCC 27606 2630 Clostridium paraputrificum 10364 Clostridium pascui 10365 Clostridium pascui 10366 Clostridium pascui 10367 Clostridium pascui 628 Clostridium perfringens 2943 Clostridium perfringens 11778 Clostridium perfringens 11779 Clostridium perfringens 11780 Clostridium perfringens 11781 Clostridium perfringens 11782 Clostridium perfringens 11783 Clostridium perfringens 11784 Clostridium perfringens 11785 Clostridium perfringens 11786 Clostridium perfringens 2632 Clostridium sardiniense 2141 [Clostridium] sordellii ATCC 9714 1985 Clostridium 4029 Clostridium 633 Clostridium sporogenes 634 Clostridium sporogenes 767 Clostridium sporogenes 1446 Clostridium sporogenes 1664 Clostridium sporogenes 1294 [Clostridium] sporosphaeroides DSM 1294 2636 Clostridium subterminale 6970 Clostridium subterminale 2485 Clostridium tertium 11744 Clostridium tetani 11745 Clostridium tetani 3979 Collinsella aerofaciens ATCC 25986 15921 Coprobacillus cateniformis JCM 10604 26242 Coprobacter fastidiosus NSB1 15641 Cryptobacterium curtum DSM 15641 20708 Dichelobacter nodosus A198 23057 Dichelobacter nodosus 3992 Dorea formicigenerans ATCC 27755 27370 Dysgonomonas macrotermitis 16107 Eggerthella sinensis 25476 Enorma massiliensis phI 3989 Holdemanella biformis DSM 3989 3983 Faecalitalea cylindroides ATCC 27803 3991 [Eubacterium] dolichum DSM 3991 2593 Eubacterium limosum 2594 Eubacterium limosum 18759 Eubacterium minutum ATCC 700079 27210 Falsiporphyromonas endometrii 1645 Filifactor villosus 6740 Flavonifractor plautii DSM 6740 19678 Fusobacterium necrophorum subsp. funduliforme ATCC 51357 19507 Fusobacterium nucleatum subsp. vincentii ATCC 49256 19508 Fusobacterium nucleatum subsp. fusiforme ATCC 51190 15657 Guggenheimella bovis 15480 Hespellia stercorisuis DSM 15480 22815 Jonquetella anthropi DSM 22815 22070 Leptotrichia trevisanii DSM 22070 1672 Megamonas hypermegale DSM 1672 19944 Megamonas rupellensis DSM 19944 3998 Mogibacterium timidum DSM 3998 23919 Murdochiella asaccharolytica 21255 Negativicoccus succinicivorans 24967 Parabacteroides chartae 29073 Parabacteroides chinchillae 19448 Parabacteroides goldsteinii DSM 19448 = WAL 12034 23371 Parabacteroides gordonii DSM 23371 16106 Paraeggerthella hongkongensis JCM 14552 19731 Paraprevotella clara YIT 11840 19681 Paraprevotella xylaniphila YIT 11841 3032 Peptoniphilus asaccharolyticus 20463 Peptoniphilus asaccharolyticus DSM 20463 2949 Peptostreptococcus anaerobius VPI 4330 = DSM 2949 23041 Peptostreptococcus russellii 20707 Porphyromonas asaccharolytica DSM 20707 23058 Porphyromonas bennonis DSM 23058 = JCM 16335 15663 Porphyromonas gulae DSM 15663 23370 Porphyromonas levii DSM 23370 23386 Porphyromonas somerae DSM 23386 23387 Porphyromonas uenonis DSM 23387 = JCM 13868 23917 Bacteroides heparinolyticus ATCC 35895 13386 Prevotella nigrescens ATCC 33563 1897 Propionibacterium acnes DSM 1897 15818 Propionibacterium australiense 23940 Pseudoflavonifractor capillosus ATCC 29799 21147 Pyramidobacter piscolens W5455 23594 Selenomonas bovis DSM 23594 19578 Selenomonas noxia ATCC 43541 2479 Selenomonas sp. ATCC 33150 5675 Tissierella praeacuta An organic-rich liquic culture medium comprised of lean ground beef hydrolysate. To this, casitone, yeast extract, potassium phosphate, resazurin, cysteine, and cysteine hydrochloride are added. Prepared under an atmosphere of dinitrogen. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium78.pdf 78. CHOPPED MEAT MEDIUM Ground beef (fat free) 500.0 g Distilled water 1000.0 ml NaOH 1 N 25.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: Casitone 30.0 g Yeast extract 5.0 g K2HPO4 5.0 g Resazurin 1.0 mg Boil, cool under nitrogen atmosphere, add 0.5 g/l cysteine and adjust pH to 7.0. To make medium anoxic boil it, cool under nitrogen atmosphere, add 0.5 g/l cysteine hydrochloride and adjust pH to 7.0. Dispense anoxically 7 ml medium into Hungate tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121˚C for 30 min. For agar slants use 15 g agar per 1000.0 ml medium. For DSM 5566 add to the autoclaved medium 1 g/l NaHCO3 from a sterile, anoxic stock solution (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture. Adjust final pH of medium to 7.2. In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving: Haemin solution (see below) 10.00 ml Vitamin K1 or Vitamin K3 solution (see below) 0.20 ml Haemin solution: Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. Vitamin K1 solution: Dissolve 0.1 ml of vitamin K1/K3 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. Vitamin K3 solution: Dissolve 5 mg/ml of vitamin K3 in 10 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. © 2011 DSMZ GmbH - All rights reserved chopped meat medium DSMZ Medium 78a Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium78a.pdf 78a. CHOPPED MEAT MEDIUM FOR TREPONEMA SP. To the ready medium 78, add 50 ml/l amino acid solution and 5 ml/l vitamin solution. These solutions must not be prepared under anaerobic conditions. Amino acid solution (filter sterilized): L-Histidine 0.6 g L-Serine 0.5 g L-Glutamine 0.7 g Distilled water 50.0 ml Vitamin solution: Vitamin B12 50 mg Pantothenic acid 50 mg Riboflavin 50 mg Pyridoxamine-HCl 10 mg Biotin 20 mg Folic acid 20 mg Nicotinic acid 25 mg Nicotine amide 25 mg α-lipoic acid 50 mg p-aminobenzoic acid 50 mg Thiamine-HCl x 2 H2O 50 mg Distilled water 1000 ml Stir for some hours, filter sterilize the solution. © 2010 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 78, except that an amino acid solution and vitamin solution are added. chopped meat medium for Treponema sp. DSM strains: 12168 Treponema brennaborense DSM 12168 DSMZ Medium 80 An organic-rich, solid culture medium comprised of peptone, beef extract, glycerol, soil extract, and agar. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium80.pdf 80. GLYCEROL-SOIL MEDIUM Peptone 5.0 g Beef extract 3.0 g Glycerol 20.0 g Soil extract 150.0 ml Distilled water 850.0 ml Agar 15.0 g Adjust pH to 7.0. Soil extract is prepared by sieving air dried garden soil through a coarse sieve and autoclaving 400 g with 960 ml of distilled water at 121˚C for one hour. After the mixture is cool and settled, the supernatant is carefully decanted, filtered through paper, autoclaved in 200 ml quantities, and stored at room temperature until cleared by sedimentation. © 2007 DSMZ GmbH - All rights reserved DSM strains: 43067 Actinomadura madurae NBRC 14623 43236 Actinomadura madurae 2354 Gordonia alkanivorans 43247 Gordonia bronchialis DSM 43247 43249 Gordonia terrae 43342 Gordonia terrae 43231 Mycobacterium conceptionense 43212 Mycobacterium gordonae 43213 Mycobacterium gordonae 43222 Mycobacterium parascrofulaceum 43239 Mycobacterium phlei DSM 43239 = CCUG 21000 535 Pseudonocardia autotrophica 43103 Pseudonocardia autotrophica 658 Pseudonocardia petroleophila 43193 Pseudonocardia petroleophila glycerol-soil medium Carrine Blank DSMZ Medium 79 Carrine Blank DSM strains: 621 Leucothrix mucor 5127 Leucothrix mucor DSM 2157 41682 Streptomyces hiroshimensis An organic-rich, liquid culture medium containing tryptone and synthetic seawater. Leptothrix medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium79.pdf 79. LEUCOTHRIX MEDIUM Tryptone 10.00 g Synthetic sea water 1000.00 ml Synthetic sea water: NaCl 24.00 g MgCl2 x 6 H2O 11.00 g Na2SO4 4.00 g CaCl2 x 6 H2O 2.00 g KCl 0.70 g KBr 0.10 g H3BO3 0.03 g NaSiO3 x 9 H2O 5.00 mg SrCl2 x 6 H2O 0.04 g NaF 3.00 mg NH4NO3 2.00 mg Fe3PO4 x 4 H2O 1.00 mg Distilled water 1000.00 ml Adjust pH to 7.8. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 82 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium82.pdf 82. BHI-GLUCOSE MEDIUM Brain heart infusion 18.5 g Glucose 5.0 g Agar 12.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved Brain Heart Infusion-glucose medium DSM strains: 43117 Actinomadura pelletieri 43118 Actinomadura pelletieri 43031 Actinoplanes auranticolor 43046 Actinoplanes missouriensis 43019 Actinoplanes philippinensis 43045 Agromyces ramosus 20651 Corynebacterium minutissimum 43037 Dermatophilus congolensis 43043 Intrasporangium calvum DSM 43043 43218 Mycobacterium diernhoferi 43058 Mycobacterium 43024 Nocardia brevicatena NBRC 12119 43005 Nocardia cyriacigeorgica 43003 Nocardia farcinica 43010 Nocardia otitidiscaviarum 43242 Nocardia otitidiscaviarum 43069 Nocardia 43027 Pseudonocardia thermophila 43188 Rhodococcus erythropolis 43113 Saccharopolyspora rectivirgula 43114 Saccharopolyspora rectivirgula 2072 Streptococcus pyogenes 2073 Streptococcus pyogenes 2074 Streptococcus pyogenes An organic-rich, solid culture medium comprised of brain heart infusion, glucose, and agar. BHI-glucose medium DSMZ Medium 81.1 A minerals-salts, solid culture medium that contains potassium phosphate, sodium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, manganese chloride, sodium metavanadate, trace elements, agar, ferric ammonium citrate and sodium bicarbonate. Prepared under an atmosphere of dioxygen, carbon dioxide, dihydrogen, and dinitrogen. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium81.pdf 81. MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) Solution A: KH2PO4 2.300 g Na2HPO4 x 2 H2O 2.900 g Distilled water 50.000 ml Solution B: NH4Cl 1.000 g MgSO4 x 7 H2O 0.500 g CaCl2 x 2 H2O 0.010 g MnCl2 x 4 H2O 0.005 g NaVO3 x H2O 0.005 g Trace element sol. SL-6 (see medium 27) 5.000 ml Distilled water 915.000 ml Agar (if necessary) 20.000 g Solution C: Ferric ammonium citrate 0.050 g Distilled water 20.000 ml Solutions A, B, C are autoclaved separately for 15 min at 121˚C, cooled down to 50˚C and then mixed aseptically with 5.0 ml filter-sterilized standard vitamin solution (see below) and 10.0 ml filter-sterilized 5% NaHCO3 (pH 7-8).The final pH of this medium should be 6.8 without adjustment. For chemolithotrophic growth incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 60% H2 and 28% N2. For heterotrophic growth supplement the mineral medium with an appropriate carbon source (0.2% carbohydrate or 0.1% organic acid). For growth on nitrogen-free medium, omit NH4Cl and incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 10% H2 and 78% N2 or heterotrophically under 2% (v/v) O2 and 98% N2. For more details see Ref. 1515 and Ref. 3363. For DSM 21436 adjust pH to 5.0 and use a gas atmosphere of 10% O2, 10% CO2, 40% H2 and 40% N2 with an overpressure of 2 bar. Standard vitamin solution: Riboflavin 10.000 mg Thiamine-HCl x 2 H2O 50.000 mg Nicotinic acid 50.000 mg Pyridoxine-HCl 50.000 mg Ca-pantothenate 50.000 mg Biotin 0.100 mg Folic acid 0.200 mg Vitamin B12 1.000 mg Distilled water 100.000 © 2011 DSMZ GmbH - All rights reserved DSM 21436 is Thiomonas islandica DSMZ Medium 81.1 -< for DSM 21436 DSMZ Medium 81 A minerals salts, liquid culture medium containing potassium phosphate, sodium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, manganese chloride, sodium metavanate, ferric ammonium citrate, sodium carbonate and trace elements. Prepared under an atmosphere of dioxygen, carbon dioxide, hydrogen, and dinitrogen. mineral medium for chemolithotrophic growth (H-3) DSM strains: 653 Achromobacter ruhlandii 550 Acidovorax facilis 620 Acidovorax facilis 649 Acidovorax facilis 3314 Acidovorax 2625 Alcaligenes 5537 Alcaligenes 334 Ancylobacter aquaticus 2454 Ancylobacter aquaticus 2456 Ancylobacter aquaticus 1108 Ancylobacter polymorphus 2457 Ancylobacter polymorphus 2667 Ancylobacter polymorphus 3910 Ancylobacter polymorphus 3911 Ancylobacter polymorphus 3912 Ancylobacter polymorphus 3913 Ancylobacter polymorphus 3914 Ancylobacter polymorphus 1106 Ancylobacter 1107 Ancylobacter 2668 Ancylobacter 2669 Ancylobacter 1277 Ancylobacter vacuolatus 1124 Azohydromonas australica DSM 1124 1122 Azohydromonas lata 1123 Azohydromonas lata 1721 Azohydromonas 1722 Azohydromonas 1723 Azohydromonas 2787 Nitrospirillum amazonense 2788 Nitrospirillum amazonense Y2 2789 Nitrospirillum amazonense 1838 Azospirillum lipoferum 1840 Azospirillum lipoferum 1841 Azospirillum lipoferum 1860 Azospirillum lipoferum 2291 Azospirillum lipoferum 2292 Azospirillum lipoferum 2293 Azospirillum lipoferum 2294 Azospirillum lipoferum 1726 Azospirillum sp. A1-3 1727 Azospirillum 1842 Azospirillum 16346 Beijerinckia 1724 Beijerinckia 1755 Bradyrhizobium japonicum 1756 Bradyrhizobium japonicum 1982 Bradyrhizobium japonicum 1749 Burkholderia fungorum 11915 Chrysiogenes arsenatis DSM 11915 2839 Cupriavidus metallidurans CH34 529 Cupriavidus necator 530 Cupriavidus necator 531 Cupriavidus necator 538 Ralstonia eutropha H16 539 Cupriavidus necator 540 Cupriavidus necator 541 Cupriavidus necator 428 Cupriavidus necator 430 Cupriavidus necator 515 Cupriavidus necator 516 Cupriavidus necator 517 Cupriavidus necator 518 Cupriavidus necator 542 Cupriavidus necator 543 Cupriavidus necator 544 Cupriavidus necator 545 Cupriavidus necator 546 Cupriavidus necator 547 Cupriavidus necator 551 Cupriavidus necator 30029 Cupriavidus necator 723 Derxia gummosa DSM 723 1846 Derxia gummosa 732 Herbaspirillum autotrophicum 733 Herbaspirillum autotrophicum 2913 Hydrogenobacter hydrogenophilus 15341 Hydrogenophaga defluvii 619 Hydrogenophaga flava 650 Hydrogenophaga palleronii 63 Hydrogenophaga palleronii 1034 Hydrogenophaga pseudoflava 2082 Hydrogenophaga taeniospiralis 21442 Hydrogenophilus islandicus 413 Paracoccus denitrificans 415 Paracoccus denitrificans 1405 Paracoccus denitrificans 1403 Paracoccus pantotrophus 1404 Paracoccus pantotrophus 2666 Starkeya 21436 Thiomonas islandica 645 Variovorax paradoxus 646 Variovorax paradoxus 647 Variovorax paradoxus 1070 Variovorax paradoxus 1071 Variovorax paradoxus 1072 Variovorax paradoxus 30034 Variovorax paradoxus 3770 Xanthobacter agilis 431 Xanthobacter autotrophicus 432 Xanthobacter autotrophicus 597 Xanthobacter autotrophicus 685 Xanthobacter autotrophicus 1393 Xanthobacter autotrophicus http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium81.pdf 81. MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) Solution A: KH2PO4 2.300 g Na2HPO4 x 2 H2O 2.900 g Distilled water 50.000 ml Solution B: NH4Cl 1.000 g MgSO4 x 7 H2O 0.500 g CaCl2 x 2 H2O 0.010 g MnCl2 x 4 H2O 0.005 g NaVO3 x H2O 0.005 g Trace element sol. SL-6 (see medium 27) 5.000 ml Distilled water 915.000 ml Agar (if necessary) 20.000 g Solution C: Ferric ammonium citrate 0.050 g Distilled water 20.000 ml Solutions A, B, C are autoclaved separately for 15 min at 121˚C, cooled down to 50˚C and then mixed aseptically with 5.0 ml filter-sterilized standard vitamin solution (see below) and 10.0 ml filter-sterilized 5% NaHCO3 (pH 7-8).The final pH of this medium should be 6.8 without adjustment. For chemolithotrophic growth incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 60% H2 and 28% N2. For heterotrophic growth supplement the mineral medium with an appropriate carbon source (0.2% carbohydrate or 0.1% organic acid). For growth on nitrogen-free medium, omit NH4Cl and incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 10% H2 and 78% N2 or heterotrophically under 2% (v/v) O2 and 98% N2. For more details see Ref. 1515 and Ref. 3363. For DSM 21436 adjust pH to 5.0 and use a gas atmosphere of 10% O2, 10% CO2, 40% H2 and 40% N2 with an overpressure of 2 bar. Standard vitamin solution: Riboflavin 10.000 mg Thiamine-HCl x 2 H2O 50.000 mg Nicotinic acid 50.000 mg Pyridoxine-HCl 50.000 mg Ca-pantothenate 50.000 mg Biotin 0.100 mg Folic acid 0.200 mg Vitamin B12 1.000 mg Distilled water 100.000 © 2011 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 83 Czapek peptone agar http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium83.pdf 83. CZAPEK PEPTONE AGAR Sucrose 30.00 g NaNO3 3.00 g K2HPO4 1.00 g MgSO4 x 7 H2O 0.50 g KCl 0.50 g FeSO4 x 7 H2O 0.01 g Yeast extract 2.00 g Peptone 5.00 g Agar 15.00 g Distilled water 1000.00 ml Adjust pH to 7.3. © 2007 DSMZ GmbH - All rights reserved Is an organic rich, solid culture medium comprised of sucrose, sodium nitrate, potassium phosphate, magnesium sulfate, potassium chloride, iron sulfate, yeast extract, peptone, and agar. DSM strains: 43148 Actinoplanes campanulatus 43149 Actinoplanes digitatis 43146 Actinoplanes italicus 43150 Actinoplanes lobatus 43151 Actinoplanes regularis 43147 Actinoplanes utahensis 44348 Amycolatopsis eurytherma 44349 Amycolatopsis eurytherma 44467 Amycolatopsis sacchari 44468 Amycolatopsis sacchari 44469 Amycolatopsis sacchari 44470 Amycolatopsis sacchari 44592 Amycolatopsis vancoresmycina DSM 44592 44439 Gordonia polyisoprenivorans 43062 Laceyella sacchari 44438 Micromonospora aurantiaca 43127 Micromonospora chalcea 43036 Micromonospora echinospora 43171 Micromonospora halophytica 44983 Micromonospora rifamycinica 43312 Micromonospora 43313 Micromonospora 43314 Micromonospora 43024 Nocardia brevicatena NBRC 12119 44538 Nocardia veterana 44412 Nocardiopsis alba 44516 Nocardiopsis alba 44552 Nocardiopsis alba 44550 Nocardiopsis composta 44551 Nocardiopsis composta 44497 Nocardiopsis sp. 64/93 44407 Nocardiopsis exhalans 44659 Nocardiopsis sp. DSM 44659 44450 Nocardiopsis 43039 Pilimelia anulata 43040 Pilimelia terevasa 44945 Planifilum fulgidum 43177 Planomonospora parontospora subsp. parontospora 43110 Promicromonospora citrea 44525 Pseudonocardia kongjuensis 43195 Pseudonocardia saturnea 43169 Pseudonocardia 44631 Saccharomonospora azurea NA-128 43068 Saccharomonospora azurea 43017 Saccharomonospora viridis DSM 43017 43115 Saccharomonospora viridis Carrine Blank DSMZ Medium 84 Carrine Blank An organic-rich, solid culture medium comprised of rolled oats, agar, and trace elements. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf 84. ROLLED OATS MINERAL MEDIUM Rolled oats 20.0 g Agar 20.0 g Trace element solution (see below) 1.0 ml Distilled water 1000.0 ml Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml pH 7,2 © 2007 DSMZ GmbH - All rights reserved rolled oats mineral medium DSM strains: 44705 Acrocarpospora macrocephala 44706 Acrocarpospora pleiomorpha 44982 bacterium 12202 43889 Actinoalloteichus cyanogriseus DSM 43889 45178 Actinocatenispora rupis 44816 Actinocatenispora thailandica 44254 Actinocorallia herbida 44770 Actinomadura glauciflava DSM 44770 46198 Actinomadura latina 46201 Actinomadura latina 43380 Actinomadura madurae 43381 Actinomadura madurae 44485 Actinomadura mexicana 44197 Actinomadura namibiensis 44761 Actinomadura nitritigenes 44762 Actinomadura nitritigenes 43750 Actinomadura rubrobrunea 43751 Actinomadura rubrobrunea 44003 Actinomadura 44763 Actinomadura 43765 Actinomadura vinacea 44433 Actinomadura viridilutea 46643 Actinoplanes globisporus 44926 Actinospica acidiphila 44927 Actinospica robiniae DSM 44927 44899 Catellatospora bangladeshensis 44900 Catellatospora chokoriensis 44901 Catellatospora coxensis 44128 Catenuloplanes crispus 44578 Clavisporangium rectum 44171 Glycomyces tenuis DSM 44171 44071 Herbidospora cretacea 44127 Herbidospora cretacea 44786 Kitasatospora gansuensis 44790 Kitasatospora kifunensis 44788 Kitasatospora paranensis 44943 Kitasatospora recifensis 44789 Kitasatospora terrestris 44826 Kitasatospora viridis 43870 Kutzneria albida DSM 43870 43851 Kutzneria kofuensis 43850 Kutzneria viridogrisea 43355 Laceyella putida 43353 Laceyella sacchari 43354 Laceyella sacchari 43356 Laceyella sacchari 45262 Laceyella tengchongensis 43885 Lechevalieria flava 15081 Lechevalieria xinjiangensis 44784 Longispora albida DSM 44784 44681 Microbispora corallina 44105 Microbispora griseoalba 43166 Microbispora rosea subsp. aerata 43176 Microbispora rosea subsp. aerata 43840 Microbispora rosea subsp. aerata 43025 Microbispora rosea subsp. rosea 43165 Microbispora rosea subsp. rosea 43837 Microbispora rosea subsp. rosea 43838 Microbispora rosea subsp. rosea 43839 Microbispora rosea subsp. rosea 43143 Micromonospora coerulea 43819 Micromonospora inositola 44579 Microtetraspora malaysiensis 44487 Nonomuraea asiatica 45128 Nonomuraea bangladeshensis 43767 Nonomuraea roseola 44170 Nonomuraea roseoviolacea subsp. carminata 43041 Planobispora longispora 43051 Planobispora rosea 43178 Planomonospora venezuelensis 44746 Planotetraspora silvatica 44698 Pseudonocardia chloroethenivorans 44072 Rhodococcus opacus 43401 Saccharopolyspora hirsuta 44936 Sphaerisporangium rubeum 44325 Spirilliplanes yamanashiensis 41754 Streptacidiphilus carbonis 44847 Streptacidiphilus griseisporus 44824 Streptacidiphilus luteoalbus 42024 Streptomyces abyssalis 41644 Streptomyces aculeolatus 41781 Streptomyces bungoensis 41447 Streptomyces chartreusis NRRL 3882 41827 Streptomyces costaricanus 41984 Streptomyces endophyticus 42021 Streptomyces glycovorans 40661 Streptomyces griseus 41944 Streptomyces guanduensis 43030 Streptomyces humiferus 42104 Streptomyces klenkii 41697 Streptomyces malaysiensis 42004 Streptomyces mordarskii 41804 Streptomyces reticuliscabiei 42105 Streptomyces smyrnaeus 40689 Streptomyces 40886 Streptomyces 40887 Streptomyces 40888 Streptomyces 40889 Streptomyces 40979 Streptomyces 41373 Streptomyces 41374 Streptomyces 41375 Streptomyces 41376 Streptomyces 41377 Streptomyces 41378 Streptomyces 41379 Streptomyces 41380 Streptomyces 41381 Streptomyces 41382 Streptomyces 41383 Streptomyces 41384 Streptomyces 41356 Streptomyces 41357 Streptomyces 41358 Streptomyces 41360 Streptomyces 41361 Streptomyces 41362 Streptomyces 41363 Streptomyces 41364 Streptomyces 41365 Streptomyces 41366 Streptomyces 41367 Streptomyces 41368 Streptomyces 41369 Streptomyces 41370 Streptomyces 41371 Streptomyces 41372 Streptomyces 41298 Streptomyces 41319 Streptomyces 41327 Streptomyces 41349 Streptomyces 41350 Streptomyces 41351 Streptomyces 41352 Streptomyces 41353 Streptomyces 41385 Streptomyces 41386 Streptomyces 41387 Streptomyces 41388 Streptomyces 41389 Streptomyces 41616 Streptomyces 41617 Streptomyces 41618 Streptomyces 41619 Streptomyces 41694 Streptomyces 41924 Streptomyces specialis 42057 Streptomyces staurosporininus 41679 Streptomyces sulfonofaciens 42019 Streptomyces sundarbansensis 44293 Streptomyces thermocarboxydus 41700 Streptomyces thermocoprophilus 41740 Streptomyces thermodiastaticus 42015 Streptomyces xinghaiensis S187 42022 Streptomyces xishensis 41945 Streptomyces yanglinensis 43023 Streptosporangium album 44109 Streptosporangium brasiliense 45034 Streptosporangium canum 45033 Streptosporangium sp. 7108 43948 Streptosporangium nondiastaticum 43021 Streptosporangium roseum DSM 43021 44095 Streptosporangium rubrum 43137 Streptosporangium 43138 Streptosporangium 43394 Streptosporangium 44662 Streptosporangium subroseum 43849 Streptosporangium violaceochromogenes 43872 Streptosporangium violaceochromogenes 44112 Streptosporangium vulgare 44663 Streptosporangium yunnanense 43184 Thermoactinomyces vulgaris 43372 Thermoactinomyces vulgaris 43038 Thermobispora bispora 43833 Thermobispora bispora DSM 43833 43186 Thermopolyspora flexuosa 44794 Virgisporangium aurantiacum 44793 Virgisporangium ochraceum DSMZ Medium 86 Castenholz medium Carrine Blank An organic-rich, liquid culture medium comprised of nitrilotriacetic acid, calcium sulfate, magnesium sulfate, sodium chloride, potassium nitrate, sodium nitrate, sodium phosphate, ferric chloride, manganese sulfate, zinc sulfate, boric acid, copper sulfate, sodium molybdate, cobalt chloride, tryptone, and yeast extract. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium86.pdf 86. CASTENHOLZ MEDIUM Nitrilotriacetic acid (Titriplex I) 100.00 mg CaSO4 x 2 H2O 60.00 mg MgSO4 x 7 H2O 100.00 mg NaCl 8.00 mg KNO3 103.00 mg NaNO3 689.00 mg Na2HPO4 x 2 H2O 140.00 mg FeCl3 x 6 H2O 0.47 mg MnSO4 x H2O 2.20 mg ZnSO4 x 7 H2O 0.50 mg H3BO3 0.50 mg CuSO4 x 5 H2O 25.00 μg Na2MoO4 x 2 H2O 25.00 μg CoCl2 x 6 H2O 46.00 μg Tryptone 1.00 g Yeast extract 1.00 g Distilled water 1000.00 ml Adjust pH to 8.2 with NaOH. © 2007 DSMZ GmbH - All rights reserved DSM strains: 11376 Meiothermus cerbereus DSM 11376 11377 Meiothermus cerbereus 9957 Meiothermus chliarophilus DSM 9957 9946 Meiothermus silvanus DSM 9946 14542 Meiothermus taiwanensis DSM 14542 14543 Meiothermus taiwanensis 19627 Microcella putealis 14833 Tepidimonas aquatica 14834 Thermomonas hydrothermalis 5718 Thermonema lapsum 23/9 5719 Thermonema lapsum 625 Thermus aquaticus 12092 Thermus oshimai DSM 12092 12093 Thermus scotoductus DSMZ Medium 85 DSM strains: 43238 Mycobacterium chitae glucose peptone medium An organic rich, solid culture medium comprised of yeast extract, peptone, glucose, calcium carbonate, and agar. Given the pKa of calcium carbonate (9), the pH of the solution should be about 9.0. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium85.pdf 85. GLUCOSE PEPTONE MEDIUM Yeast extract 10.0 g Bacto peptone (Difco) 20.0 g Glucose 10.0 g CaCO3 10.0 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 87 An organic-rich, liquid culture medium comprised of yeast extract, glycylglycine, sodium phosphate, magnesium sulfate, potassium nitrate, sodium nitrate, sodium chloride, calcium chloride, ferric citrate, and trace elements. Prepared under an atmosphere of dinitrogen. Chloroflexus medium (modified) Carrine Blank DSM strains: 13941 Roseiflexus castenholzii DSM 13941 9485 Chloroflexus aggregans DSM 9485 9486 Chloroflexus aggregans 635 Chloroflexus aurantiacus J-10-fl 636 Chloroflexus aurantiacus 637 Chloroflexus aurantiacus 638 Chloroflexus aurantiacus http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium87.pdf 87. CHLOROFLEXUS MEDIUM (modified) Yeast extract 1.00 g Glycyl-glycine 1.00 g Na2HPO4 x 2 H2O 0.10 g MgSO4 x 7 H2O 0.10 g KNO3 0.10 g NaNO3 0.50 g NaCl 0.10 g CaCl2 x 2 H2O 0.05 g Fe(III) citrate solution (0.1 g in 100 ml H2O) 5.00 ml Trace element solution SL-6 (see medium 27) 1.00 ml Distilled water 1050.00 ml Adjust pH to 8.2. Boil the medium under a stream of nitrogen gas for a few minutes and distribute 90 ml medium into 100 ml screw-capped bottles. Bubble each bottle with nitrogen gas and close immediately with a rubber septum and screw tight. Autoclave at 121˚C for 15 min. After autoclaving inject 1.0 ml of neutralized sulfide solution (0.015% end concentration, see medium 27) to each bottle. This medium can be stored for several months. Incubate the culture at 50˚C at a light intensity of 300 - 500 lux. For heavy cell suspension supplement periodically with sterile yeast extract solution (0.1% end concentration, for more details see Ref. 3365, 3366). © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 86a Carrine Blank DSM strains: 14542 Meiothermus taiwanensis DSM 14542 14543 Meiothermus taiwanensis modified Castenholz medium Similar to DSMZ Medium 86, except tryptone is omitted and peptone and monosodium glutamate are added. The pH is decreased to 7.8 with sodium hydroxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium86a.pdf 86a. MODIFIED CASTENHOLZ MEDIUM Use medium 86, but replace the tryptone with 3 g/l peptone and add 1 g/l mono sodium glutamate. The final pH should be adjusted to 7.8 with NaOH. Solid media is prepared by the addition of 2 - 2.5% agar. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 87a Carrine Blank Chloroflexus aggregans medium Similar to DSMZ Medium 87, except a vitamin solution is added and the pH is decreased to 7.5. DSM strains: 9485 Chloroflexus aggregans DSM 9485 9486 Chloroflexus aggregans 13941 Roseiflexus castenholz DSM 13941 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium87a.pdf 87a. CHLOROFLEXUS AGGREGANS MEDIUM To medium 87 add 1.0 ml/litre sterile vitamin solution CA. Vitamin solution CA Distilled water 100.0 ml Nicotinic acid 100.0 mg Thiamine-HCl x 2 H2O 100.0 mg Biotin 5.0 mg p-Aminobenzoic acid 50.0 mg Vitamin B12 1.0 mg Ca-pantothenate 50.0 mg Pyridoxine-HCl 50.0 mg Folic acid 50.0 mg Na3-EDTA 200.0 mg Adjust pH to 7.5. The solution is filter-sterilized. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 88.8 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88.pdf 88. SULFOLOBUS MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Yeast extract (Difco) 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Sterilize substrates separately and add to the medium after autoclaving. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. For DSM 5348 omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and ore by heating at 150°C over night. Add sulfur and ore aseptically to the autoclaved medium. For DSM 5389, DSM 7519, and DSM 12421 adjust pH of medium to 3.0 – 3.5. For DSM 6482 and DSM 10039 reduce amount of yeast extract to 0.20 g/l and supplement medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. For DSM 9789 and DSM 9790 use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. For DSM 16993 supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0. For DSM 18786 use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150 °C over night. Adjust pH of the medium to 0.8. For DSM 29038 supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5. For DSM 29099 use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 – 3.0. © 2015 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 88, except the concentration of yeast extract is reduced, and D-glucose and elemental sulfur are added. The pH is increased to 2.5-3.0. DSMZ Medium 88.8 -< for DSM 29099 DSM 29099 is Acidianus sp. RZ1 DSMZ Medium 88.7 Carrine Blank DSM 29038 is Acidianus sp. ALE 1 = Candidatus Acidianus copahuensis http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88.pdf 88. SULFOLOBUS MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Yeast extract (Difco) 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Sterilize substrates separately and add to the medium after autoclaving. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. For DSM 5348 omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and ore by heating at 150°C over night. Add sulfur and ore aseptically to the autoclaved medium. For DSM 5389, DSM 7519, and DSM 12421 adjust pH of medium to 3.0 – 3.5. For DSM 6482 and DSM 10039 reduce amount of yeast extract to 0.20 g/l and supplement medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. For DSM 9789 and DSM 9790 use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. For DSM 16993 supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0. For DSM 18786 use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150 °C over night. Adjust pH of the medium to 0.8. For DSM 29038 supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5. For DSM 29099 use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 – 3.0. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 88, except potassium tetrathionate is added. The pH is increased to 2.5. DSMZ Medium 88.7 -< for DSM 29038 DSMZ Medium 88.6 Similar to DSMZ Medium 88, except the concentration of yeast extract is decreased and a sulfide mineral is added. The pH is decreased to 0.8. DSM 18786 is Acidianus sulfidivorans JP7 Carrine Blank DSMZ Medium 88.6 -< for DSM 18786 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88.pdf 88. SULFOLOBUS MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Yeast extract (Difco) 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Sterilize substrates separately and add to the medium after autoclaving. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. For DSM 5348 omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and ore by heating at 150°C over night. Add sulfur and ore aseptically to the autoclaved medium. For DSM 5389, DSM 7519, and DSM 12421 adjust pH of medium to 3.0 – 3.5. For DSM 6482 and DSM 10039 reduce amount of yeast extract to 0.20 g/l and supplement medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. For DSM 9789 and DSM 9790 use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. For DSM 16993 supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0. For DSM 18786 use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150 °C over night. Adjust pH of the medium to 0.8. For DSM 29038 supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5. For DSM 29099 use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 – 3.0. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 88.5 DSMZ Medium 88.5 -< for DSM 16993 Carrine Blank DSM 16993 is Sulfolobus tokodaii str. 7 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88.pdf 88. SULFOLOBUS MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Yeast extract (Difco) 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Sterilize substrates separately and add to the medium after autoclaving. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. For DSM 5348 omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and ore by heating at 150°C over night. Add sulfur and ore aseptically to the autoclaved medium. For DSM 5389, DSM 7519, and DSM 12421 adjust pH of medium to 3.0 – 3.5. For DSM 6482 and DSM 10039 reduce amount of yeast extract to 0.20 g/l and supplement medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. For DSM 9789 and DSM 9790 use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. For DSM 16993 supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0. For DSM 18786 use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150 °C over night. Adjust pH of the medium to 0.8. For DSM 29038 supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5. For DSM 29099 use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 – 3.0. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 88, except D-glucose and casamino acids are added. The pH is increased to 3.0. DSMZ Medium 88.4 DSM 9789 is Picrophilus oshimae DSM 9789 DSM 9790 is Picrophilus torridus DSM 9790 Similar to DSMZ Medium 88, except the concentration of yeast extract is increased and the pH is decreased to 1.0. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88.pdf 88. SULFOLOBUS MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Yeast extract (Difco) 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Sterilize substrates separately and add to the medium after autoclaving. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. For DSM 5348 omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and ore by heating at 150°C over night. Add sulfur and ore aseptically to the autoclaved medium. For DSM 5389, DSM 7519, and DSM 12421 adjust pH of medium to 3.0 – 3.5. For DSM 6482 and DSM 10039 reduce amount of yeast extract to 0.20 g/l and supplement medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. For DSM 9789 and DSM 9790 use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. For DSM 16993 supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0. For DSM 18786 use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150 °C over night. Adjust pH of the medium to 0.8. For DSM 29038 supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5. For DSM 29099 use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 – 3.0. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 88.4 -< for DSM 9789 and DSM 9790 Carrine Blank DSMZ Medium 88.3 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88.pdf 88. SULFOLOBUS MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Yeast extract (Difco) 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Sterilize substrates separately and add to the medium after autoclaving. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. For DSM 5348 omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and ore by heating at 150°C over night. Add sulfur and ore aseptically to the autoclaved medium. For DSM 5389, DSM 7519, and DSM 12421 adjust pH of medium to 3.0 – 3.5. For DSM 6482 and DSM 10039 reduce amount of yeast extract to 0.20 g/l and supplement medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. For DSM 9789 and DSM 9790 use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. For DSM 16993 supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0. For DSM 18786 use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150 °C over night. Adjust pH of the medium to 0.8. For DSM 29038 supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5. For DSM 29099 use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 – 3.0. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 88.3 -< for DSM 6482 and DSM 10039 DSM 6482 is Sulfolobus metallicus DSM 6482 = JCM 9184 DSM 10039 is Metallosphaera prunae Similar to DSMZ Medium 88, except the amount of yeast extract is reduced and elemental sulfur is added. DSMZ Medium 88.2 Carrine Blank DSMZ Medium 88.2 -< for DSM 5389, DSM 7519, and DSM 12421 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88.pdf 88. SULFOLOBUS MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Yeast extract (Difco) 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Sterilize substrates separately and add to the medium after autoclaving. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. For DSM 5348 omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and ore by heating at 150°C over night. Add sulfur and ore aseptically to the autoclaved medium. For DSM 5389, DSM 7519, and DSM 12421 adjust pH of medium to 3.0 – 3.5. For DSM 6482 and DSM 10039 reduce amount of yeast extract to 0.20 g/l and supplement medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. For DSM 9789 and DSM 9790 use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. For DSM 16993 supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0. For DSM 18786 use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150 °C over night. Adjust pH of the medium to 0.8. For DSM 29038 supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5. For DSM 29099 use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 – 3.0. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 88, except the pH is increased to 3.0-3.5. DSM 5389 is Sulfolobus shibatae B12 DSM 7519 is Metallosphaera hakonensis JCM 8857 DSM 12421 is Sulfurisphaera ohwakuensis DSMZ Medium 88.1 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88.pdf 88. SULFOLOBUS MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Yeast extract (Difco) 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Sterilize substrates separately and add to the medium after autoclaving. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. For DSM 5348 omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and ore by heating at 150°C over night. Add sulfur and ore aseptically to the autoclaved medium. For DSM 5389, DSM 7519, and DSM 12421 adjust pH of medium to 3.0 – 3.5. For DSM 6482 and DSM 10039 reduce amount of yeast extract to 0.20 g/l and supplement medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. For DSM 9789 and DSM 9790 use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. For DSM 16993 supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0. For DSM 18786 use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150 °C over night. Adjust pH of the medium to 0.8. For DSM 29038 supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5. For DSM 29099 use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 – 3.0. © 2015 DSMZ GmbH - All rights reserved DSM 5348 is Metallosphaera sedula DSM 5348 Similar to DSMZ Medium 88, except yeast extract is omitted and elemental sulfur and a sulfide mineral are added. DSMZ Medium 88.1 -< for DSM 5348 DSMZ Medium 88 Sulfolobus medium DSM strains: 29038 Candidatus Acidianus copahuensis 18786 Acidianus sulfidivorans JP7 7519 Metallosphaera hakonensis 10039 Metallosphaera prunae 639 Sulfolobus acidocaldarius DSM 639 6482 Sulfolobus metallicus DSM 6482 = JCM 9184 5389 Sulfolobus shibatae B12 16993 Sulfolobus tokodaii str. 7 12421 Sulfurisphaera ohwakuensis 18247 Thermobacillus composti KWC4 14429 Vulcanisaeta distributa DSM 14429 14430 Vulcanisaeta souniana An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric chloride, manganese chloride, sodium tetraborate, zinc sulfate, vanadyl sulfate, cobalt sulfate, and yeast extract. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88.pdf 88. SULFOLOBUS MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Yeast extract (Difco) 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Sterilize substrates separately and add to the medium after autoclaving. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. For DSM 5348 omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and ore by heating at 150°C over night. Add sulfur and ore aseptically to the autoclaved medium. For DSM 5389, DSM 7519, and DSM 12421 adjust pH of medium to 3.0 – 3.5. For DSM 6482 and DSM 10039 reduce amount of yeast extract to 0.20 g/l and supplement medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. For DSM 9789 and DSM 9790 use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. For DSM 16993 supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0. For DSM 18786 use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150 °C over night. Adjust pH of the medium to 0.8. For DSM 29038 supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5. For DSM 29099 use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 – 3.0. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 88b DSM strains: 2337 Thermofilum librum V24N medium An organic-rich, liquid culture medium comprised of elemental sulfur, yeast extract, soluble starch, resazurin, and sodium sulfide. Prepared under an atmosphere of dinitrogen. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88b.pdf 88b. V24N MEDIUM Prepare medium 88 without yeast extract, then add (per litre): Sulfur, powdered 1.0 g Yeast extract 0.2 g Starch, soluble 2.0 g Resazurin 0.4 mg Na2S x 9 H2O 0.5 g Adjust the pH to 6.0 with H2SO4 (25% v/v). Prepare the medium under 100% N2 gas phase and distribute anaerobically into rubber stoppered tubes or bottles (presterilized by autoclaving). The medium is sterilized by heating 2 hours at 85 ˚C on each of two following days. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 88a.1 Carrine Blank DSM 2161 is Desulfurococcus mobilis DSM 2161 DSM 2162 is Desulfurococcus mucosus DSM 2162 Similar to DSMZ Medium 88a, except the concentration of yeast extract is increased and the pH is increased to 5.5. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88a.pdf 88a. SULFOLOBUS MEDIUM (ANAEROBIC) (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Sulfur, powder 10.00 g Yeast extract (Difco) 0.50 g Na2S x 9 H2O 0.50 g Demineralized water 1000.00 ml Dissolve ingredients (except sulfur, yeast extract and sulfide), adjust pH of the salt solution at room temperature to 4.0 using 1 N H2SO4 and sparge medium with 100% N2 gas to make it anoxic. Dispense medium under same gas atmosphere in anoxic vials and autoclave. Steam sulfur for 3 hr on each of 3 successive days. Add sulfur aseptically to the autoclaved medium while retaining anoxic conditions. Add yeast extract and sulfide from sterile anoxic stock solutions prepared under N2 gas. Prior to inoculation check pH and adjust to 4.0, if necessary. For DSM 2161 and DSM 2162 use 1.00 g/l yeast extract and adjust pH of medium to 5.5. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 88a.1 -< for DSM 2161 and DSM 2162 DSMZ Medium 88a Similar to DSMZ Medium 88, except elemental sulfur and sodium sulfide are added. Prepared under an atmosphere of dinitrogen and the pH is increased to 4.0. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88a.pdf 88a. SULFOLOBUS MEDIUM (ANAEROBIC) (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Sulfur, powder 10.00 g Yeast extract (Difco) 0.50 g Na2S x 9 H2O 0.50 g Demineralized water 1000.00 ml Dissolve ingredients (except sulfur, yeast extract and sulfide), adjust pH of the salt solution at room temperature to 4.0 using 1 N H2SO4 and sparge medium with 100% N2 gas to make it anoxic. Dispense medium under same gas atmosphere in anoxic vials and autoclave. Steam sulfur for 3 hr on each of 3 successive days. Add sulfur aseptically to the autoclaved medium while retaining anoxic conditions. Add yeast extract and sulfide from sterile anoxic stock solutions prepared under N2 gas. Prior to inoculation check pH and adjust to 4.0, if necessary. For DSM 2161 and DSM 2162 use 1.00 g/l yeast extract and adjust pH of medium to 5.5. © 2015 DSMZ GmbH - All rights reserved Sulfolobus medium (anaerobic) DSM strains: none found in BacDive DSMZ Medium 90 An organic-rich, solid culture medium comprised of malt extract, soy peptone, and agar. DSM strains: none found in BacDive database http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium90.pdf 90. MALT EXTRACT PEPTONE AGAR Malt extract 30.0 g Soya peptone 3.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 5.6. Sterilize at 121˚C for 10 min © 2007 DSMZ GmbH - All rights reserved malt extract peptone agar Carrine Blank DSMZ Medium 91 An organic-rich, solid culture medium comprised of casein peptone, tryptic digest (casitone), yeast extract, sodium lactate, and agar. DSM strains: 20272 Propionibacterium acidipropionici 20273 Propionibacterium acidipropionici 20271 Propionibacterium freudenreichii subsp. freudenreichii 20270 Propionibacterium freudenreichii subsp. shermanii 20274 Propionibacterium jensenii 20275 Propionibacterium jensenii 20278 Propionibacterium jensenii 20279 Propionibacterium jensenii 20535 Propionibacterium jensenii DSM 20535 20276 Propionibacterium thoenii DSM 20276 20277 Propionibacterium thoenii Carrine Blank Propionibacterium agar http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium91.pdf 91. PROPIONIBACTERIUM AGAR Casein peptone, tryptic digest 10.0 g Yeast extract 5.0 g Na-lactate 10.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0 - 7.2. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 92a modified medium 92 DSM strains: 15826 Luteococcus peritonei http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium92a.pdf 92a. MODIFIED MEDIUM 92 Prepare medium 92. Add vitamin solution (see medium 131). © 2007 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 92, except that a vitamin solution is added. DSMZ Medium 92 An organic-rich, liquid culture medium comprised of tryptic soy broth, yeast extract, and agar. trypticase soy yeast extract medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium92.pdf 92. TRYPTICASE SOY YEAST EXTRACT MEDIUM Trypticase soy broth 30.0 g Yeast extract 3.0 g Agar, if necessary 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0 - 7.2. © 2012 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 95 DSM strains: 684 Desulfuromonas acetoxidans DSM 684 1675 Desulfuromonas acetoxidans 1676 Desulfuromonas acetoxidans A minerals-salts, liquid culture medium containing potassium phospahte, ammonium chloride, magnesium sulfate, magnesium chloride, sodium chloride, calcium chloride, sodium sulfate, trace elements, sodium malate, yeast extract, resazurin, ehtanol, sodium bicarbonate, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium95.pdf 95. DESULFUROMONAS ACETOXIDANS MEDIUM Solution A: KH2PO4 1.00 g NH4Cl 0.30 g MgSO4 x 7 H2O 1.00 g MgCl2 x 6 H2O 2.00 g NaCl 20.00 g CaCl2 x 2 H2O 0.10 g Na2SO4 1.77 g Trace element solution SL-4 (see medium 14) 10.00 ml Na2-DL-malate 2.66 g Yeast extract 0.50 g Resazurin 1.00 mg Distilled water 940.00 ml Adjust pH to 6.0 with 2 N NaOH. Solution B: Ethanol 0.30 ml Distilled water 2.70 ml Solution C: NaHCO3 1.85 g Distilled water 40.00 ml Solution D: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Bring solution A to the boil, then cool down to room temperature under 100% N2 gas atmosphere. Dispense medium under same gas atmosphere in culture vessels and sterilize by autoclaving. Solutions B and D are autoclaved separately under 100% N2 gas atmosphere. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. The medium is completed before use by adding appropriate amounts of solutions B, C and D to solution A. Final pH is adjusted to 7.2. © 2014 DSMZ GmbH - All rights reserved Desulfuromonas acetoxidans medium DSMZ Medium 115 Vibrio natriegens medium DSM strains: Carrine Blank An organic-rich, solid microbiological culture medium comprised of peptone, meat extract, sodium chloride, and agar. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium115.pdf 115. VIBRIO NATRIEGENS MEDIUM To medium 1 add 1.5% NaCl. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 114 An organic-rich, solid microbiological culture medium comprised of peptone, meat extract, sodium chloride, and agar. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium114.pdf 114. PARACOCCUS HALODENITRIFICANS MEDIUM To medium 1 add 6% NaCl. © 2007 DSMZ GmbH - All rights reserved DSM strains: Paracoccus halodenitrificans medium DSMZ Medium 113a Sulfurimonas hongkongensis medium Similar to DSMZ Medium 113, except sodium chloride is added such that the salinity is increased to marine salinity levels. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium113a.pdf 113a. SULFURIMONAS HONGKONGENSIS MEDIUM Solution A: NaCl 25.0 g KH2PO4 2.0 g KNO3 2.0 g NH4Cl 1.0 g MgSO4 x 7 H2O 0.8 g Trace element solution SL-4 (see medium 14) 2.0 ml Distilled water 930.0 ml Adjust pH to 7.0 with NaOH. Solution B: Na2S2O3 x 5 H2O 5.0 g Distilled water 40.0 ml Solution C: NaHCO3 1.0 g Distilled water 20.0 ml Solution D: FeSO4 x 7 H2O 2.0 mg H2SO4 (0.1 N) 1.0 ml Solution E: Vitamins solution (see medium 141) 10.0 ml Solutions A, B and D are prepared under 100% N2 gas atmosphere and sterilized by autoclaving at 121˚C for 15 min. Solution C is sterilized by filtration or by autoclaving in a tightly closed vessel under an atmosphere of 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. After sterilization combine the five solutions and distribute as required under 100% N2 gas atmosphere. For solid medium add 15 g agar to solution A. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: DSMZ Medium 113 DSM strains: Carrine Blank A minerals-salts, liquid culture medium comprised of potassium phosphate, potassium nitrate, ammonium chloride, magnesium sulfate, trace elements, sodium selenite, sodium bicarbonate, and ferrous sulfate. Prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium113.pdf 113. THIOBACILLUS DENITRIFICANS MEDIUM Solution A: KH2PO4 2.0 g KNO3 2.0 g NH4Cl 1.0 g MgSO4 x 7 H2O 0.8 g Trace element solution SL-4 (see medium 14) 2.0 ml Distilled water 940.0 ml Adjust pH to 7.0 with NaOH. Solution B: Na2S2O3 x 5 H2O 5.0 g Distilled water 40.0 ml Solution C: NaHCO3 1.0 g Distilled water 20.0 ml Solution D: FeSO4 x 7 H2O 2.0 mg H2SO4 (0.1 N) 1.0 ml Solutions A, B and D are sterilized separately by autoclaving at 121˚C for 15 min under nitrogen atmosphere. Solution C is sterilized by filtration or by autoclaving in a tightly closed vessel under an atmosphere of 80% N2 and 20% CO2 gas atmosphere. After sterilization combine the four solutions and distribute as required under nitrogen atmosphere. For solid medium add 15 g agar to solution A. © 2010 DSMZ GmbH - All rights reserved Thiobacillus denitrificans medium DSMZ Medium 120a.1 Carrine Blank Similar to DSMZ Medium 120a, except the concentration of sodium bicarbonate is increased, the concentration of methanol is decreased, and the pH is increased slightly to 6.8-7.0. Prepared under an atmosphere of dihydrogen and carbon dioxide. DSM 4556 is Methanosarcina mazei http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120a.pdf 120a. METHANOSARCINA BARKERI MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Resazurin 0.50 mg NaHCO3 0.85 g Vitamin solution (see medium 141) 10.00 ml Methanol 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins are prepared under 100% N2 gas and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 6.5 - 6.8. For DSM 4556 increase amount of NaHCO3 to 2.00 g/l to achieve a pH of 6.8 – 7.0 and add only 5.00 ml/l methanol. After inoculation pressurize vials with 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. For DSM 10334 and DSM 13486 increase amount of NaHCO3 to 2.00 g/l to achieve a pH of 6.8 – 7.0 and add only 5.00 ml/l methanol. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 120a.1 -< for DSM 4556 DSMZ Medium 118 DAP-nutrient agar Carrine Blank Similar to DSMZ Medium 1, except diaminopimelic acid (2,6-diaminopimelic acid) is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium118.pdf 118. DAP-NUTRIENT AGAR To medium 1 add 100 mg DL-diaminopimelic acid. © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 119 DSM strains: Methanobacterium medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium sulfate, sodium chloride, ammonium chloride, calcium chloride, ferrous sulfate, trace elements, yeast extract, sodium acetate, sodium formate, sludge fluid, fatty acids, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, dinitrogen, and carbon dioxide. Carrine Blank DSMZ Medium 119.1 Similar to DSMZ Medium 119, except coenzyme M is added. DSM 1093 is Methanobrevibacter ruminantium M1 DSMZ Medium 119.1 -< for DSM 1093 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 119.2 Similar to DSMZ Medium 119, except pH is reduced to 6.5 and the atmosphere is dihydrogen and carbon dioxide. DSMZ Medium 119.2 -< for DSM 2030 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 2030 is Thermoanaerobacter kivui DSMZ Medium 119.3 Carrine Blank DSM 6216 is Methanoculleus bourgensis Similar to DSMZ Medium 119, except the concentration of sodium acetate is increased. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 119.3 -< for DSM 6216 DSMZ Medium 119.4 Similar to DSMZ Medium 119, except sodium sulfate is added. DSM 7057 is Desulfovibrio sp. G11 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 119.4 -< for DSM 7057 DSMZ Medium 119.5 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 119.5 -< for DSM 7256 DSM 7256 is Methanobrevibacter oralis Similar to DSMZ Medium 119, except sludge fluid is omitted and clarified rumen fluid is added. DSMZ Medium 119.6 Carrine Blank DSM 9575 is Methanobacterium bryantii http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 119, except the atmosphere is dihydrogen and carbon dioxide. DSMZ Medium 119.6 -< for DSM 9575 DSMZ Medium 119.7 DSM 15163 is Methanobrevibacter acididurans Carrine Blank Similar to DSMZ Medium 119, except the pH is reduced to 6.0. DSMZ Medium 119.7 -< for DSM 15163 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 119.8 DSMZ Medium 119.8 -< for DSM 16632 and DSM 16643 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved DSM 16632 is Methanobrevibacter olleyae DSM 16643 is Methanobrevibacter millerae Similar to DSMZ Medium 119, except sludge fluid is omiited, and trypicase peptone, vitamin solution, and clarified rumen fluid is added. Carrine Blank DSMZ Medium 119.9 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 119.9 -< for DSM 25720 DSM 25720 is Methanomassiliicoccus luminyensis B10 Similar to DSMZ Medium 119, except selenite-tungstate solution and methanol are added. The pH is increased to 7.5 and the atmosphere is dihydrogen and carbon dioxide. DSMZ Medium 119.10 DSM 25824 is Methanobrevibacter boviskoreani JH1 Similar to DSMZ Medium 119, except coenzyme is added and the atmosphere is dihydrogen and carbon dioxide. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 119.10 -< for DSM 25824 DSMZ Medium 119.11 DSM 25939 is Methanobacterium flexile Carrine Blank DSMZ Medium 119.11 -< for DSM 25939 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 119, except the pH is increased to 7.2 and the atmosphere is dihydrogen and carbon dioxide. DSMZ Medium 119.12 DSMZ Medium 119.12 -< for DSM 25945 Carrine Blank DSM 25945 is Methanobacterium movens http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 119, except the pH is increased to 7.4 and the atmosphere is dihydrogen and carbon dioxide. DSMZ Medium 104b http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of trypticase peptone, pepsi-digested peptone from meat (meat peptone), yeast extract, salt solution, rezasurin, L-cysteine hydrochloride, and D-glucose. Prepared under an atmosphere of dinitrogen. DSM strains: PY + X medium Carrine Blank DSMZ Medium 104b.1 DSMZ Medium 104b.1 -< for DSM 1736 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved DSM 1736 is Dendrosporobacter quercicolus DSM 1736 Similar to DSMZ Medium 104b, except glucose is omitted and D-fructose is added. Carrine Blank DSMZ Medium 104b.2 Carrine Blank DSM 6555 is [Clostridium] xylanolyticum Similar to DSMZ Medium 104b, except glucose is omitted and xylan is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 104b.2 -< for DSM 6555 DSMZ Medium 104b.3 Carrine Blank DSM 12857 is [Clostridium] amygdalinum DSM 13479 is Hungatella hathewayi DSM 13479 DSM 13480 is Hungatella hathewayi DSMZ Medium 104b.3 -< for DSM 12857, DSM 13479 and DSM 13480 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 104b, except glucose is omitted. DSMZ Medium 104b.4 DSM 13181 is Anaerobaculum mobile DSM 13181 DSMZ Medium 104b.4 -< for DSM 13181 Similar to DSMZ Medium 104b, except the concentration of glucose is reduced. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 104b.5 DSM 14760 is Phascolarctobacterium faecium DSM 14760 DSM 14761 is Phascolarctobacterium faecium DSM 14762 is Phascolarctobacterium faecium Similar to DSMZ Medium 104b, except glucose is omitted and sodium succinate is added. DSMZ Medium 104b.5 -< for DSM 14760, DSM 14761 and DSM 14762 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 104b.6 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved DSM 19022 is Lutispora thermophila DSM 19022 Similar to DSMZ Medium 104b, except glucose is ommitted and sodium pyruvate is added. DSMZ Medium 104b.6 -< for DSM 19022 Carrine Blank DSMZ Medium 104b.7 Similar to DSMZ Medium 104b, except glucose is omitted and vitamins and sodium glutamate (monosodium glutamate) are added. DSMZ Medium 104b.7 -< for DSM 21120 DSM 21120 is Anaerosphaera aminiphila DSM 21120 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 104b.8 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 104b, except glucose is omitted and the pH is increased to 8.5. DSMZ Medium 104b.8 -< for DSM 21650 Carrine Blank DSM 21650 is Proteiniborus ethanoligenes DSM 21650 DSMZ Medium 104b.9 Similar to DSMZ Medium 104b, except glucose is omitted and D-fructose is added. The pH is increased to 7.5-8.0. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 21761 is Alkaliphilus oremlandii OhILAs DSMZ Medium 104b.9 -< for DSM 21761 DSMZ Medium 104b.10 DSM 24749 is Youngiibacter fragilis 232.1 Similar to DSMZ Medium 104b, except glucose is omitted and D-fructose and sodium chloride are added. The pH is increased to 7.3-7.5. DSMZ Medium 104b.10 -< for DSM 24749 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 104b.11 Carrine Blank DSMZ Medium 104b.11 -< for DSM 26752 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved DSM 26752 is Keratinibaculum paraultunense Similar to DSMZ Medium 104b, except sodium carbonate is added and the pH is increased to 8.0. DSMZ Medium 104b.12 DSM 27512 is Clostridium sp. ST07-YE Carrine Blank DSMZ Medium 104b.12 -< for DSM 27512 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 104b, except sodium carbonate is added and the pH increased to 8.5. DSMZ Medium 104b.13 DSM 28571 is Clostridium oryzae DSMZ Medium 104b.13 -< for DSM 28571 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 104b, except glucose is omitted and D-cellobiose (beta-cellobiose) is added. The pH is lowered to 6.5. Carrine Blank garden soil extract for DSMZ Medium 98 Carrine Blank Soil extract formed from garden soil. Formed by making a water extract of garden soil and sodium carbonate, followed by autoclaving and centrifugation. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium98.pdf 98. RHIZOBIUM MEDIUM Yeast extract 1.0 g Mannitol 10.0 g Agar 15.0 g Soil extract 200.0 ml Distilled water 800.0 ml Adjust pH to 7.0. Soil extract: Air-dried garden soil 80.0 g Na2CO3 0.2 g Distilled water 200.0 ml Autoclave soil suspension for one hour at 121˚C. To obtain a clear supernatant allow to settle and centrifuge. Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 104a Carrine Blank An organic-rich, liquid culture medium comprised of trypticase peptone, pepsi-digested peptone from meat (meat peptone), yeast extract, sodium chloride, salt solution, rezasurin, L-cysteine hydrochloride, D-glucose and sodium thiosulfate. Prepared under an atmosphere of dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104a.pdf 104a. ANAEROBACULUM THERMOTERRENUM MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g NaCl 8.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 1.0 g Na2S2O3 x 5 H2O 2.5 g Distilled water 1000.0 ml Dissolve ingredients (except glucose, thiosulfate and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose and thiosulfate from sterile, anoxic stock solutions prepared under N2. Final pH of the completed medium should be 7.0. © 2007 DSMZ GmbH - All rights reserved DSM strains: Anaerobaculum thermoterrenum medium DSMZ Medium 98 Carrine Blank DSM strains: 11603 Aquamicrobium defluvii 15010 marine alpha proteobacterium V4.MO.26 16623 Bradyrhizobium canariense 11554 Bradyrhizobium elkanii USDA 76 1755 Bradyrhizobium japonicum 1756 Bradyrhizobium japonicum 1982 Bradyrhizobium japonicum 30131 Bradyrhizobium japonicum 19632 Bradyrhizobium jicamae 24092 Bradyrhizobium liaoningense 16931 Bradyrhizobium pachyrhizi 1984 Bradyrhizobium 5966 Bradyrhizobium 5968 Bradyrhizobium 5969 Bradyrhizobium 13375 Sinorhizobium arboris 13376 Sinorhizobium arboris 13377 Sinorhizobium arboris 5851 Sinorhizobium fredii 5852 Sinorhizobium fredii 5824 Sinorhizobium fredii 13372 Sinorhizobium kostiense 13373 Sinorhizobium kostiense 13374 Sinorhizobium kostiense 1981 Sinorhizobium meliloti 6057 Sinorhizobium meliloti 6057 Sinorhizobium meliloti 30135 Sinorhizobium meliloti 30136 Sinorhizobium meliloti 23913 Sinorhizobium meliloti 23914 Sinorhizobium meliloti 11273 Sinorhizobium saheli 26426 Ensifer sojae CCBAU 05684 11282 Sinorhizobium terangae 100023 Mesorhizobium camelthorni 17287 Mesorhizobium chacoense 1978 Mesorhizobium ciceri 29666 Mesorhizobium hawassense 6573 Mesorhizobium huakuii 2626 Mesorhizobium loti 2627 Mesorhizobium loti NZP2037 2628 Mesorhizobium loti 5235 Mesorhizobium loti 5236 Mesorhizobium loti 6046 Mesorhizobium loti 11555 Mesorhizobium mediterraneum UPM-Ca36 100038 Mesorhizobium muleiense 29667 Mesorhizobium qingshengii 100022 Mesorhizobium robiniae 100039 Mesorhizobium sangaii 29845 Mesorhizobium silamurunense 28320 Mesorhizobium tamadayense 11417 Mesorhizobium tianshanense 11587 Rhizobacter dauci 1111 Rhizobium aggregatum 11541 Rhizobium etli CFN 42 19332 Rhizobium fabae 11542 Neorhizobium galegae bv. orientalis str. HAMBI 540 11917 Rhizobium hainanense 26427 Rhizobium herbae 29977 Rhizobium laguerreae 1980 Rhizobium leguminosarum 2108 Rhizobium leguminosarum 6039 Rhizobium leguminosarum 6040 Rhizobium leguminosarum 6041 Rhizobium leguminosarum 6042 Rhizobium leguminosarum 6043 Rhizobium leguminosarum 6044 Rhizobium leguminosarum 6045 Rhizobium leguminosarum 30133 Rhizobium leguminosarum 30138 Rhizobium leguminosarum 30139 Rhizobium leguminosarum 30141 Rhizobium leguminosarum 30143 Rhizobium leguminosarum 1983 Bradyrhizobium lupini 30134 Bradyrhizobium lupini 30140 Bradyrhizobium lupini 28449 Rhizobium mesoamericanum CCGE 501 26482 Rhizobium petrolearium 1379 Rhizobium phaseoli 6038 Rhizobium phaseoli 30137 Rhizobium phaseoli 30132 Rhizobium pisi 26483 Rhizobium pseudoryzae 22668 Rhizobium pusense 26376 Rhizobium rosettiformans W3 26438 Rhizobium skierniewicense Ch11 1725 Rhizobium sp. AM1 2729 Rhizobium 2914 Rhizobium 6049 Rhizobium 6050 Rhizobium 6051 Rhizobium 6052 Rhizobium 6599 Rhizobium 10169 Rhizobium 11272 Rhizobium 11419 Rhizobium 14623 Rhizobium sullae 14624 Rhizobium sullae 11418 Rhizobium tropici 25379 Rhizobium tubonense 26437 Rhizobium vallis 25378 Rhizobium vignae 19334 Shinella kummerowiae 15007 Sinorhizobium americanum 15008 Sinorhizobium americanum http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium98.pdf 98. RHIZOBIUM MEDIUM Yeast extract 1.0 g Mannitol 10.0 g Agar 15.0 g Soil extract 200.0 ml Distilled water 800.0 ml Adjust pH to 7.0. Soil extract: Air-dried garden soil 80.0 g Na2CO3 0.2 g Distilled water 200.0 ml Autoclave soil suspension for one hour at 121˚C. To obtain a clear supernatant allow to settle and centrifuge. Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved Rhizobium medium An organic-rich, solid culture medium containing yeast extract, mannitol, agar, and soil extract. DSMZ Medium 97 Halobacterium medium DSM strains: 3752 Haloarcula marismortui 11927 Haloarcula quadrata 3756 Haloarcula vallismortis 668 Halobacterium salinarum 669 Halobacterium salinarum 670 Halobacterium salinarum 671 Halobacterium salinarum 3754 Halobacterium salinarum 22414 Halobacterium salinarum 3753 Halobacterium sp. 3750 Halobacterium sp. 1307 Halococcus morrhuae DSM 1307 1308 Halococcus morrhuae 1309 Halococcus morrhuae 1310 Halococcus morrhuae 1411 Haloferax mediterranei ATCC 33500 3757 Haloferax volcanii DS2 15367 Halomonas magadiensis 1137 Halorubrum saccharovorum DSM 1137 13077 Natrialba aegyptia DSM 13077 3751 Natrinema pallidum DSM 3751 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium97.pdf 97. HALOBACTERIUM MEDIUM Casamino acids 7.50 g Yeast extract 10.00 g Na3-citrate 3.00 g KCl 2.00 g MgSO4 x 7 H2O 20.00 g FeSO4 x 7 H2O 0.05 g MnSO4 x H2O 0.20 mg NaCl 250.00 g Agar 20.00 g Distilled water 1000.00 ml Adjust pH to 7.4. Add the agar after dissolving all ingredients in the water and adjustment of pH. © 2007 DSMZ GmbH - All rights reserved Carrine Blank An organic-rich, solid culture medium comprised of casamino acids, yeast extract, sodium citrate, potassium chloride, magnesium sulfate, ferrous sulfate, manganese sulfate, sodium chloride, and agar. DSMZ Medium 104c An organic-rich, liquid culture medium comprised of trypticase peptone, pepsin-digested peptone from meat (meat peptone), yeast extract, resazurin, salt solution, L-cysteine hydrochloride, sodium carbonate, and D-glucose. Prepared under an atmosphere of carbon dioxide and dinitrogen. Carrine Blank PY + X medium (N2/CO2) PY+X medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104c.pdf 104c. PY + X MEDIUM (N2/CO2) Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Resazurin 1.0 mg Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g Na2CO3 2.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except cysteine, carbonate and glucose), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Add cysteine, dispense under same gas atmosphere in culture vessels and autoclave. Adjust pH of autoclaved medium to 7.0 with an anoxic, sterile stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture. Prior to inoculation add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 753 replace glucose with 10.0 g/l maltose. For DSM 7320 adjust pH to 6.3. © 2014 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 104c.1 Similar to DSMZ Medium 104c, except glucose is omitted and maltose is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104c.pdf 104c. PY + X MEDIUM (N2/CO2) Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Resazurin 1.0 mg Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g Na2CO3 2.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except cysteine, carbonate and glucose), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Add cysteine, dispense under same gas atmosphere in culture vessels and autoclave. Adjust pH of autoclaved medium to 7.0 with an anoxic, sterile stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture. Prior to inoculation add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 753 replace glucose with 10.0 g/l maltose. For DSM 7320 adjust pH to 6.3. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSM 753 is [Clostridium] leptum DSM 753 DSMZ Medium 104c.1 -< for DSM 753 DSMZ Medium 104c.2 DSMZ Medium 104c.2 -< for DSM 7320 Similar to DSMZ Medium 104c, except the pH is lowered to 6.3. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104c.pdf 104c. PY + X MEDIUM (N2/CO2) Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Resazurin 1.0 mg Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g Na2CO3 2.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except cysteine, carbonate and glucose), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Add cysteine, dispense under same gas atmosphere in culture vessels and autoclave. Adjust pH of autoclaved medium to 7.0 with an anoxic, sterile stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture. Prior to inoculation add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 753 replace glucose with 10.0 g/l maltose. For DSM 7320 adjust pH to 6.3. © 2014 DSMZ GmbH - All rights reserved DSM 7320 is Clostridium roseum Carrine Blank DSMZ Medium 104d Clostridium halophilum medium (betaine) Clostridium halophilum medium DSM strains: An organic-rich, liquid culture medium comprised of trypicase peptone, pepsin-digested peptone from meat (meat peptone), yeast extract, salt solution, sodium chloride, magnesium sulfate, betaine (glycine betaine), resazurin, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104d.pdf 104d. CLOSTRIDIUM HALOPHILUM MEDIUM (BETAINE) Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml NaCl 60.0 g MgSO4 x 7 H2O 5.0 g Betaine x H2O 6.0 g Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.3. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. Adjust pH of completed medium to 7.3 – 7.5. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 105 An organic-rich, solid culture medium comprised of glucose, yeast extract, calcium carbonate, and agar. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium105.pdf 105. GLUCONOBACTER OXYDANS MEDIUM Glucose 100.0 g Yeast extract 10.0 g CaCO3 20.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 6.8. 20 g calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies. © 2007 DSMZ GmbH - All rights reserved Gluconobacter oxydans medium Carrine Blank DSM strains: DSMZ Medium 109 Similar to DSMZ Medium 27, except sodium malate (disodium malate), sodium thiosulfate, and sodium sulfide are added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium109.pdf 109. RHODOPSEUDOMONAS SULFOVIRIDIS MEDIUM To medium 27 add 0.1% sodium malate and 0.05% sodium thiosulfate. Adjust pH to 6.8 and sterilize at 121˚C for 15 min. The liquid medium is sterilized in rubber stoppered or screw-capped bottles under anaerobic conditions (Ref. 3365). After sterilization neutralized sterile sodium sulfide solution is added to a final concentration of 0.03% (see medium 27). © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank Rhodopseudomonas sulfoviridis medium DSMZ Medium 108 An organic-rich, solid culture medium comprised of glucose, ammonium sulfate, potassium phossphate, magnesium sulfate, potassium chloride, calcium nitrate, ferrous sulfate, and agar. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium108.pdf 108. THIOBACILLUS ACIDOPHILUS MEDIUM Glucose 10.00 g (NH4)2SO4 3.00 g KH2PO4 0.50 g MgSO4 x 7 H2O 1.00 g KCl 0.10 g Ca(NO3)2 x 4 H2O 18.00 mg FeSO4 x 7 H2O 0.01 mg Agar 15.00 g Distilled water 1000.00 ml In liquid medium, the pH should be adjusted to 3.5 with H2SO4. In solid medium, the pH should be adjusted to 4.5 with H2SO4 after autoclaving the medium. Sterilize the glucose and the basal solution separately. © 2007 DSMZ GmbH - All rights reserved Thiobacillus acidophilus medium DSM strains: sludge fluid for DSMZ Medium 119 Carrine Blank Sludge fluid where yeast extract has been added, and has been gassed with dinitrogen, then centrifuged and autoclaved. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved fatty acid mixture for DSMZ Medium 119 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved Carrine Blank A fatty acid solution comprised of valeric acid, isovaleric acid, 2-methylbutyric acid, and isobutyric acid. fatty acid solution A defined organic solution containing short-chain fatty acids or short-chain fatty acid anions. Carrine Blank DSMZ Medium 1310 An organic-rich, liquid culture medium comprised of casitone, yeast extract, D-glucose, cellobiose, maltose, sodium lactate, clarified rumen fluid, potassium phosphate, ammonium sulfate, sodium chloride, calcium chloride, magnesium sulfate, sodium carbonate, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared over an atmosphere of carbon dioxide and dinitrogen. Butyrivibrio M2 medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1310.pdf 1310. BUTYRIVIBRIO M2 MEDIUM Casitone (BACTO) 10.00 g Yeast extract 2.50 g D-Glucose 2.00 g Cellobiose 2.00 g Maltose 2.00 g Na-DL-lactate 5.00 g Rumen fluid, clarified (see below) 200.00 ml K2HPO4 0.05 g KH2PO4 0.05 g (NH4)2SO4 0.10 g NaCl 0.10 g CaCl2 x 2 H2O 0.01 g MgSO4 x 7 H2O 0.01 g Resazurin 0.50 mg Na2CO3 4.00 g L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 800.00 ml Dissolve ingredients (except carbonate, cysteine and sulfide), boil medium for 1 min, then cool to room temperature under 100% CO2 gas atmosphere. Dispense under same gas atmosphere in culture vessels and autoclave. Add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Add cysteine and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas. Adjust the final pH of the medium to 6.5 – 6.8. Clarified rumen fluid: Rumen fluid from cow or sheep is filtered through muslin, autoclaved at 121 °C for 15 min. and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by flushing with nitrogen for 15 min. and then stored frozen at -20 °C. © 2014 DSMZ GmbH - All rights reserved Carrine Blank clarified rumen fluid for DSMZ Medium 1310 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1310.pdf 1310. BUTYRIVIBRIO M2 MEDIUM Casitone (BACTO) 10.00 g Yeast extract 2.50 g D-Glucose 2.00 g Cellobiose 2.00 g Maltose 2.00 g Na-DL-lactate 5.00 g Rumen fluid, clarified (see below) 200.00 ml K2HPO4 0.05 g KH2PO4 0.05 g (NH4)2SO4 0.10 g NaCl 0.10 g CaCl2 x 2 H2O 0.01 g MgSO4 x 7 H2O 0.01 g Resazurin 0.50 mg Na2CO3 4.00 g L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 800.00 ml Dissolve ingredients (except carbonate, cysteine and sulfide), boil medium for 1 min, then cool to room temperature under 100% CO2 gas atmosphere. Dispense under same gas atmosphere in culture vessels and autoclave. Add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Add cysteine and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas. Adjust the final pH of the medium to 6.5 – 6.8. Clarified rumen fluid: Rumen fluid from cow or sheep is filtered through muslin, autoclaved at 121 °C for 15 min. and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by flushing with nitrogen for 15 min. and then stored frozen at -20 °C. © 2014 DSMZ GmbH - All rights reserved Clarified rumen fluid that is filtered through muslin, made anoxic by flusing with dinitrogen. salt solution for DSMZ Medium 104 An inorganic salts solution comprised of calcium chloride, magnesium sulfate, potasium phosphate, sodium bicarbonate, and sodium chloride. Has a salinity of 1.475%. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104.pdf 104. PYG MEDIUM (modified) Trypticase peptone 5.00 g Peptone 5.00 g Yeast extract 10.00 g Beef extract 5.00 g Glucose 5.00 g K2HPO4 2.00 g Tween 80 1.00 ml Cysteine-HCl x H2O 0.50 g Resazurin 1.00 mg Salt solution (see below) 40.00 ml Distilled water 950.00 ml Haemin solution (see below) 10.00 ml Vitamin K1 solution (see below) 0.20 ml The vitamin K1, haemin solution and the cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and autoclave. Salt solution: CaCl2 x 2 H2O 0.25 g MgSO4 x 7 H2O 0.50 g K2HPO4 1.00 g KH2PO4 1.00 g NaHCO3 10.00 g NaCl 2.00 g Distilled water 1000.00 ml Haemin solution: Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water. Store refrigerated. Vitamin K1 solution: Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. © 2009 DSMZ GmbH - All rights reserved DSMZ Medium 143 DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium143.pdf 143. CLOSTRIDIUM SP. LA1 MEDIUM Crotonic acid 6.00 g NaOH 0.30 g (NH4)2HPO4 0.15 g K2HPO4 0.10 g MgCl2 x 6 H2O 0.03 g CaCl2 x 2 H2O 0.04 g NH4Cl 0.05 g MgSO4 x 7 H2O 0.60 mg MnSO4 x 2 H2O 0.40 mg FeSO4 x 7 H2O 0.40 mg (NH4)6Mo7O24 x 4 H2O 10.00 mg Biotin 0.04 mg p-Aminobenzoic acid 0.80 mg Tryptone (Difco) 1.00 g Yeast extract 1.00 g Resazurin 1.00 mg K2CO3 4.00 g Distilled water 1000.00 ml Dissolve ingredients (except K2CO3) and sparge medium with 100% N2 gas to make it anoxic (30 – 45 min). An anoxic stock solution of the potassium carbonate is autoclaved separately under 80% N2 and 20% CO2 gas mixture and thereafter added to the sterile medium. Adjust pH of the completed medium to 6.8 - 7.0. Prior to inoculation add 10 - 20 mg/l sodium dithionite from a 5% (w/v) stock solution that has been freshly prepared under N2 and sterilized by filtration. © 2014 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of crotonic acid, sodium hydroxide, ammonium phosphate, potassium phosphate, magnesium chloride, calcium chloride, ammonium chloride, magnesium sulfate, manganese sulfate, ferrous suflate, ammonium molybdate, p-aminobenzoic acid (4-aminobenzoic acid), tyrptone, yeast extract, resazurin, sodium dithionite, and potassium carbonate. Prepared under an atmosphere of dinitrogen and carbon dioxide. Clostridium sp. LA1 medium DSMZ Medium 144.2 Carrine Blank Similar to DSMZ Medium 144, except D-glucose is omitted. DSMZ Medium 144.2 -< for DSM 12299 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium144.pdf 144. THERMOANAEROBIUM MEDIUM NH4Cl 0.90 g NaCl 0.90 g MgCl2 x 6 H2O 0.40 g KH2PO4 0.75 g K2HPO4 1.50 g Trace element solution (see below) 9.00 ml FeSO4 x 7 H2O 3.00 mg Yeast extract 3.00 g Tryptone or Trypticase 10.00 g Resazurin 0.50 mg Vitamin solution (see medium 141) 5.00 ml D-Glucose 5.00 g Na2S x 9 H2O 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except vitamins, glucose and sulfide), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere in culture vessels and autoclave. Add glucose, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under N2. Adjust pH of final medium to 7.2 - 7.4. For DSM 7040 omit tryptone and reduce amount of yeast extract to 1.00 g/l. For DSM 12299 omit D-glucose. Trace element solution: Nitrilotriacetic acid 12.80 g FeCl2 x 4 H2O 0.20 g MnCl2 x 4 H2O 0.10 g CoCl2 x 6 H2O 0.17 g CaCl2 x 2 H2O 0.10 g ZnCl2 0.10 g CuCl2 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 26.00 mg NaCl 1.00 g Na2SeO3 x 5 H2O 0.02 g Distilled water 1000.00 ml First, dissolve nitrilotriacetic acid and adjust to pH 6.5 with KOH. © 2015 DSMZ GmbH - All rights reserved DSM 12299 is Thermoanaerobacter siderophilus SR4 DSMZ Medium 144.1 DSMZ Medium 144.1 -< for DSM 7040 Similar to DSMZ Medium 144, except tryptone is omitted and the concentration of yeast extract is decreased. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium144.pdf 144. THERMOANAEROBIUM MEDIUM NH4Cl 0.90 g NaCl 0.90 g MgCl2 x 6 H2O 0.40 g KH2PO4 0.75 g K2HPO4 1.50 g Trace element solution (see below) 9.00 ml FeSO4 x 7 H2O 3.00 mg Yeast extract 3.00 g Tryptone or Trypticase 10.00 g Resazurin 0.50 mg Vitamin solution (see medium 141) 5.00 ml D-Glucose 5.00 g Na2S x 9 H2O 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except vitamins, glucose and sulfide), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere in culture vessels and autoclave. Add glucose, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under N2. Adjust pH of final medium to 7.2 - 7.4. For DSM 7040 omit tryptone and reduce amount of yeast extract to 1.00 g/l. For DSM 12299 omit D-glucose. Trace element solution: Nitrilotriacetic acid 12.80 g FeCl2 x 4 H2O 0.20 g MnCl2 x 4 H2O 0.10 g CoCl2 x 6 H2O 0.17 g CaCl2 x 2 H2O 0.10 g ZnCl2 0.10 g CuCl2 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 26.00 mg NaCl 1.00 g Na2SeO3 x 5 H2O 0.02 g Distilled water 1000.00 ml First, dissolve nitrilotriacetic acid and adjust to pH 6.5 with KOH. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 7040 is Caldicellulosiruptor acetigenus DSM 7040 DSMZ Medium 144 An organic-rich, liquid culture medium comprised of ammonium chloride, sodium chloride, magnesium chloride, potassium phosphate, trace elements, ferrous sulfate, yeast extract, tryptone (or trypicase peptone), resazurin, vitamins, D-glucose, and sodium sulfide. Prepared under an atmosphere of dinitrogen. Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium144.pdf 144. THERMOANAEROBIUM MEDIUM NH4Cl 0.90 g NaCl 0.90 g MgCl2 x 6 H2O 0.40 g KH2PO4 0.75 g K2HPO4 1.50 g Trace element solution (see below) 9.00 ml FeSO4 x 7 H2O 3.00 mg Yeast extract 3.00 g Tryptone or Trypticase 10.00 g Resazurin 0.50 mg Vitamin solution (see medium 141) 5.00 ml D-Glucose 5.00 g Na2S x 9 H2O 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except vitamins, glucose and sulfide), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere in culture vessels and autoclave. Add glucose, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under N2. Adjust pH of final medium to 7.2 - 7.4. For DSM 7040 omit tryptone and reduce amount of yeast extract to 1.00 g/l. For DSM 12299 omit D-glucose. Trace element solution: Nitrilotriacetic acid 12.80 g FeCl2 x 4 H2O 0.20 g MnCl2 x 4 H2O 0.10 g CoCl2 x 6 H2O 0.17 g CaCl2 x 2 H2O 0.10 g ZnCl2 0.10 g CuCl2 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 26.00 mg NaCl 1.00 g Na2SeO3 x 5 H2O 0.02 g Distilled water 1000.00 ml First, dissolve nitrilotriacetic acid and adjust to pH 6.5 with KOH. © 2015 DSMZ GmbH - All rights reserved Thermoanaerobium medium DSMZ Medium 142.1 DSMZ Medium 142.1 -< for DSM 12346, DSM 12350, DSM 12351, DSM 12352, DSM 12353, and DSM 12354 Similar to DSMZ Medium 142, except cobalamin is omitted and the pH is increased to 7.3-7.6. Carrine Blank DSM 12346 is Thiomicrospira crunogena DSM 12350 is Thiomicrospira kuenenii DSM 12350 DSM 12351 is Thiomicrospira frisia DSM 12352 is Thiomicrospira chilensis DSM 12352 DSM 12353 is Thiomicrospira crunogena DSM 12354 is Thiomicrospira crunogena http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium142.pdf 142. THIOMICROSPIRA PELOPHILA MEDIUM NaCl 25.00 g (NH4)2SO4 1.00 g MgSO4 x 7 H2O 1.50 g CaCl2 x 2 H2O 0.42 g Trace element solution (see medium 69) 0.20 ml Bromothymol blue 4.00 mg K2HPO4 0.50 g Na2S2O3 x 5 H2O 5.00 g Vitamin B12 15.00 μg Distilled water 1000.00 ml Adjust pH to 7.2. K2HPO4 and Na2S2O3 are autoclaved separately each in 10% of the final volume. Filter sterilize the vitamin B12. Adjust the completed medium to pH 7.2 with sterile 0.4% (w/v) Na2CO3 solution. Alternatively, "aged sea water" may be used (see medium 246), supplemented by the indicated amounts of (NH4)2SO4, bromothymol blue, K2HPO4, sodium thiosulfate and vitamin B12. Growth of most strains of Thiomicrospira is more reliable if the medium is prepared under a 80% N2 and 20% CO2 gas atmosphere to make it anoxic and then filled under air atmosphere in Hungate-type tubes (5 ml per vial). The pH is adjusted with a sterile stock solution of NaHCO3 (10% w/v) after autoclaving. For DSM 12346, DSM 12350, DSM 12351, DSM 12352, DSM 12353, and DSM 12354 omit vitamins and adjust pH of final medium to 7.3 – 7.6. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 142 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium142.pdf 142. THIOMICROSPIRA PELOPHILA MEDIUM NaCl 25.00 g (NH4)2SO4 1.00 g MgSO4 x 7 H2O 1.50 g CaCl2 x 2 H2O 0.42 g Trace element solution (see medium 69) 0.20 ml Bromothymol blue 4.00 mg K2HPO4 0.50 g Na2S2O3 x 5 H2O 5.00 g Vitamin B12 15.00 μg Distilled water 1000.00 ml Adjust pH to 7.2. K2HPO4 and Na2S2O3 are autoclaved separately each in 10% of the final volume. Filter sterilize the vitamin B12. Adjust the completed medium to pH 7.2 with sterile 0.4% (w/v) Na2CO3 solution. Alternatively, "aged sea water" may be used (see medium 246), supplemented by the indicated amounts of (NH4)2SO4, bromothymol blue, K2HPO4, sodium thiosulfate and vitamin B12. Growth of most strains of Thiomicrospira is more reliable if the medium is prepared under a 80% N2 and 20% CO2 gas atmosphere to make it anoxic and then filled under air atmosphere in Hungate-type tubes (5 ml per vial). The pH is adjusted with a sterile stock solution of NaHCO3 (10% w/v) after autoclaving. For DSM 12346, DSM 12350, DSM 12351, DSM 12352, DSM 12353, and DSM 12354 omit vitamins and adjust pH of final medium to 7.3 – 7.6. © 2015 DSMZ GmbH - All rights reserved Thiomicrospira pelophila medium A minerals-salts, liquid culture medium comprised sodium chloride, ammonium sulfate, magnesium sulfate, calcium chloride, trace elements, bromothymol blue, potassium phosphate, sodium thiosulfate, and cobalamin. Medium has midly reducing redox if prepared under dinitrogen and carbon dioxide. DSM strains: DSMZ Medium 142a DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium142a.pdf 142a. THIOMICROSPIRA PSYCHROPHILA MEDIUM NaCl 15.00 g (NH4)2SO4 1.00 g MgSO4 x 7 H2O 1.50 g CaCl2 x 2 H2O 0.42 g Trace element solution SL-10 (see medium 320) 1.00 ml Bromothymol blue 4.00 mg K2HPO4 0.50 g Na2S2O3 x 5 H2O 5.00 g Seven vitamins solution (see medium 503) 1.00 ml Distilled water 1000.00 ml Dissolve ingredients (except phosphate, thiosulfate and vitamins), adjust pH to 6.0 and autoclave. Phosphate and thiosulfate are autoclaved separately each in 5% of the final volume. The vitamin solution is sterilized by filtration. Adjust the completed medium to pH 7.6 with sterile 5% (w/v) NaHCO3 solution. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Thiomicrospira psychrophila medium A minerals-salts, liquid culture medium comprised of sodium chloride, ammonium sulfate, magnesium sulfate, calcium chloride, trace elements, bromothymol blue, potassium phosphate, sodium thiosulfate, and vitamins. DSMZ Medium 141c.3 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141c.pdf 141c. GTA13 MEDIUM (N2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (OXOID) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Trimethylammonium chloride 5.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, trimethylammonium chloride, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% N2 and 20% CO2 gas mixture. After sterilization add trimethylammonium chloride, cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 7.0 – 7.5. For DSM 2834, DSM 3028, DSM 3029, DSM 3318, DSM 4659, DSM 6636, DSM 13159, DSM 15192, DSM 21213, and DSM 21339 replace trimethylammonium chloride by 5.00 ml/l methanol added to the autoclaved medium from a sterile anoxic stock solution. Adjust pH of final medium to 6.8 – 7.2. For DSM 5309 omit trimethylammonium chloride from the medium. For DSM 6564 adjust pH of completed medium to 6.8. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 141c, except the pH is reduced to 6.8. DSMZ Medium 141c.3 -< for DSM 6564 DSM 6564 is Methanosarcina siciliae HI350 Carrine Blank DSMZ Medium 141c.2 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141c.pdf 141c. GTA13 MEDIUM (N2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (OXOID) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Trimethylammonium chloride 5.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, trimethylammonium chloride, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% N2 and 20% CO2 gas mixture. After sterilization add trimethylammonium chloride, cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 7.0 – 7.5. For DSM 2834, DSM 3028, DSM 3029, DSM 3318, DSM 4659, DSM 6636, DSM 13159, DSM 15192, DSM 21213, and DSM 21339 replace trimethylammonium chloride by 5.00 ml/l methanol added to the autoclaved medium from a sterile anoxic stock solution. Adjust pH of final medium to 6.8 – 7.2. For DSM 5309 omit trimethylammonium chloride from the medium. For DSM 6564 adjust pH of completed medium to 6.8. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 141c.2 -< for DSM 5309 Similar to DSMZ Medium 141c, except trimethylammonium is omitted. DSM 5309 is Thermosipho africanus Ob7 Carrine Blank DSMZ Medium 141c.1 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141c.pdf 141c. GTA13 MEDIUM (N2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (OXOID) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Trimethylammonium chloride 5.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, trimethylammonium chloride, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% N2 and 20% CO2 gas mixture. After sterilization add trimethylammonium chloride, cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 7.0 – 7.5. For DSM 2834, DSM 3028, DSM 3029, DSM 3318, DSM 4659, DSM 6636, DSM 13159, DSM 15192, DSM 21213, and DSM 21339 replace trimethylammonium chloride by 5.00 ml/l methanol added to the autoclaved medium from a sterile anoxic stock solution. Adjust pH of final medium to 6.8 – 7.2. For DSM 5309 omit trimethylammonium chloride from the medium. For DSM 6564 adjust pH of completed medium to 6.8. © 2015 DSMZ GmbH - All rights reserved DSM 2834 is Methanosarcina acetivorans C2A DSM 3028 is Methanosarcina siciliae T4/M DSM 3029 is Methanolobus vulcani DSM 3318 is Methanosarcina mazei C16 DSM 4659 is not in www.dsmz. de. Is Methanosarcina sp. WH1 in StrainInfo. DSM 6636 is Methanosarcina sp. MTP4 DSM 13159 is Methanosarcina calensis str. Cali DSM 15192 is Methanosarcina sp. DSM 21213 is Methanolobus profundi DSM 21339 is Methanolobus zinderi Similar to DSMZ Medium 141c, except trimethylammonium chloride is omitted and ethanol added. The pH is reduced to 6.8-7.2. DSMZ Medium 141c.1 -< for DSM 2834, DSM 3028, DSM 3029, DSM 3318, DSM 4659, DSM 6636, DSM 13159, DSM 15192, DSM 21213, and DSM 21339 Carrine Blank DSMZ Medium 141c http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141c.pdf 141c. GTA13 MEDIUM (N2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (OXOID) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Trimethylammonium chloride 5.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, trimethylammonium chloride, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% N2 and 20% CO2 gas mixture. After sterilization add trimethylammonium chloride, cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 7.0 – 7.5. For DSM 2834, DSM 3028, DSM 3029, DSM 3318, DSM 4659, DSM 6636, DSM 13159, DSM 15192, DSM 21213, and DSM 21339 replace trimethylammonium chloride by 5.00 ml/l methanol added to the autoclaved medium from a sterile anoxic stock solution. Adjust pH of final medium to 6.8 – 7.2. For DSM 5309 omit trimethylammonium chloride from the medium. For DSM 6564 adjust pH of completed medium to 6.8. © 2015 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of potassium chloride, manganese chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, ammonium acetate, yeast extract, trypticase peptone, resazurin, sodium bicarbonate, trimethylammonium chloride, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. GTA13 medium (N2/CO2) DSM strains: Carrine Blank DSMZ Medium 141b Carrine Blank Methanoculleus sp. medium An organic-rich, liquid culture medium comprised of potassium chloride, manganese chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, ammonium acetate, yeast extract, trypticase peptone, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141b.pdf 141b. METHANOCULLEUS SP. MEDIUM KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 6.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (OXOID) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. Adjust pH of final medium to 6.8 – 7.0. If the medium is being used without gas mixture overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. © 2014 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 141a Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141a.pdf 141a. METHANOCOCCUS SP. MEDIUM KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Resazurin 1.00 mg Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except vitamins, cysteine and sulfide), sparge medium with 80% H2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclave anoxically under the same gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. Adjust pH of final medium to 5.5 – 5.7. © 2014 DSMZ GmbH - All rights reserved DSM strains: Methanococcus sp. medium An organic-rich, liquid culture medium comprised of potassium chloride, manganese chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, ammonium acetate, resazurin, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide. DSMZ Medium 140 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium140.pdf 140. CLOSTRIDIUM CELLOBIOPARUM MEDIUM KH2PO4 0.50 g NaCl 1.00 g (NH4)2 SO4 0.50 g MgSO4 x 7 H2O 0.10 g CaCl2 x 2 H2O 0.10 g K2HPO4 0.50 g Clarified rumen fluid or sludge fluid (see medium 119) 300.00 ml Cellobiose 5.00 g NaHCO3 10.00 g Resazurin 1.00 mg L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 700.00 ml Dissolve ingredients (except bicarbonate, cellobiose and reducing agents), bring medium to the boil, then cool to room temperature under 100% CO2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. Add cellobiose, sulfide and cysteine from sterile anoxic stock solutions prepared under N2 and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Cellobiose is sterilized by filtration. Adjust pH of completed medium to 6.8. © 2014 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of potassium phosphate, sodium chloride, ammonium sulfate, mangesium sulfate, calcium chloride, clarified rumen fluid or sludge fluid, cellobiose, sodium bicarbonate, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: Carrine Blank Clostridium cellobioparum emdium DSMZ Medium 139 DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium139.pdf 139. CLOSTRIDIUM HISTOLYTICUM MEDIUM Trypticase soy broth (BBL) 15.00 g Proteose peptone (Difco) 50.00 g Na2HPO4 9.00 g KH2PO4 1.92 g MgSO4 x 7 H2O 0.08 g Distilled water 1000.00 ml Dissolve ingredients, adjust pH to 7.2, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. © 2014 DSMZ GmbH - All rights reserved Clostridium histolyticum medium An organic-rich, liquid culture medium comprised of trypticase soy broth (nn organic-rich, liquid culture medium comprised of pancreatic digest of casein (casitone), papaic digest of soybean (soy peptone), dextrose (D-glucose), sodium chloride, and dipotassium phosphate (potassium dibasic phosphate), proteose peptone, sodium phosphate, potassium phosphate, and magnesium sulfate. Carrine Blank DSMZ Medium 138 DSM strains: An organic-rich, liquid culture medium comprised of yeast extract and Lindane (gamma-hexachlorocyclohexane). Lindane medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium138.pdf 138. LINDANE MEDIUM Yeast extract 5.0 g Hexachlorocyclo-hexane (Lindane) 0.1 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 136 Veillonella medium An organic-rich, liquid culture medium comprised of trypticase (trypticase peptone), yeast extract, sodium lactate, sodium thioglycolate, tween 80 (polysorbate 80), glucose, putrescine, and resazurin. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium136.pdf 136. VEILLONELLA MEDIUM Trypticase (BBL) 5.00 g Yeast extract 3.00 g Na-(DL)-lactate 7.50 g Na-thioglycolate 0.75 g Tween 80 1.00 g Glucose 1.00 g Putrescine 3.00 mg Resazurin 1.00 mg Distilled water 1000.00 ml Adjust pH to 7.5 with solid K2CO3. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 137 mineral lactate medium An organic-rich, liquid culture medium comprised of sodium lactate, potassium phosphate, ammonium chloride, magnesium sulfate, sodium chloride, calcium chloride, and trace elements. DSM strains: mineral lactate medium (low carbon content) Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium137.pdf 137. MINERAL LACTATE MEDIUM (low carbon content) Na-lactate 0.50 g K2HPO4 x 3 H2O 1.13 g KH2PO4 0.88 g NH4Cl 1.00 g MgSO4 x 7 H2O 0.50 g NaCl 1.00 g CaCl2 x 2 H2O 5.00 mg Trace element solution (see medium 69) 1.20 ml Distilled water 1000.00 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 135b An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, magnesium sulfate, trace elements, yeast extract, resazurin, sodium carbonate, sodium syringate, vitamins, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium135b.pdf 135b. ACETOBACTERIUM DEHALOGENANS MEDIUM NH4Cl 1.00 g KH2PO4 0.33 g K2HPO4 0.45 g MgSO4 x 7 H2O 0.10 g Trace element solution (see medium 141) 20.00 ml Yeast extract 2.00 g Resazurin 1.00 mg Na2CO3 5.00 g Na-syringate 1.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except carbonate, syringate, vitamins and cysteine, bring to the boil and cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Thereafter, dispense in anoxic culture vessels under same gas atmosphere and autoclave. Add syringate, vitamins and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the completed medium should be at 7.4. After growth has been started add an additional amount of syringate to reach a final concentration of 2.00 g/l. © 2015 DSMZ GmbH - All rights reserved Acetobacterium dehalogenans medium Carrine Blank DSMZ Medium 135a Acetobacterium medium (marine) Carrine Blank An organic-rich, liquid culture medium comprised of sodium chloride, ammonium chloride, potassium phosphate, magnesium sulfate, trace elements, yeast extract, resazurin, sodium bicarbonate, ethylene glycol, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium135a.pdf 135a. ACETOBACTERIUM MEDIUM (MARINE) NaCl 20.00 g NH4Cl 1.00 g KH2PO4 0.33 g K2HPO4 0.45 g MgSO4 x 7 H2O 0.10 g Trace element solution (see medium 141) 20.00 ml Yeast extract 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Ethylene glycol 1.25 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, ethylene glycol, vitamins, cysteine and sulfide, bring to the boil and cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add bicarbonate (solid) and equilibrate the medium with the gas until a pH of around 7.4 is reached. Then distribute and autoclave under the same gas atmosphere. Before use add ethylene glycol, vitamins (sterilized by filtration), cysteine and sulfide from anoxic sterile stock solutions prepared under 100% N2 gas. Adjust pH of completed medium to 7.0 – 7.2. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 135.2 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium135.pdf 135. ACETOBACTERIUM MEDIUM NH4Cl 1.00 g KH2PO4 0.33 g K2HPO4 0.45 g MgSO4 x 7 H2O 0.10 g Trace element solution (see medium 141) 20.00 ml Yeast extract 2.00 g Resazurin 1.00 mg NaHCO3 10.00 g D-Fructose 10.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, fructose, vitamins, cysteine and sulfide, bring to the boil and cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add bicarbonate (solid) and equilibrate the medium with the gas until a pH of around 7.4 is reached. Then distribute and autoclave under the same gas atmosphere. Before use adjust the pH to 8.2 by adding a sterile anoxic stock solution of carbonate (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture (c. 0.25 ml per 10 ml medium) and add fructose, vitamins (sterilized by filtration), cysteine and sulfide from anoxic sterile stock solutions prepared under 100% N2. For autotrophic growth fructose is omitted and a gas atmosphere of 80% H2 and 20% CO2 is used. For DSM 1974 and DSM 7417 use only 1.00 g/l of NaHCO3 and omit Na2CO3 to reach a final pH of 6.5. For DSM 4132 use fructose as substrate at a final concentration of 1.00 g/l. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 135, except the concentration of fructose is decreased. DSM 4132 is Acetobacterium malicum Carrine Blank DSMZ Medium 135.2 -< for DSM 4132 DSMZ Medium 135.1 Carrine Blank Similar to DSMZ Medium 135, except sodium carbonate is omitted and the concentration of sodium bicaronbate is reduced. The pH is reduced to 6.5. DSMZ Medium 135.1 -< for DSM 1974 and DSM 7417 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium135.pdf 135. ACETOBACTERIUM MEDIUM NH4Cl 1.00 g KH2PO4 0.33 g K2HPO4 0.45 g MgSO4 x 7 H2O 0.10 g Trace element solution (see medium 141) 20.00 ml Yeast extract 2.00 g Resazurin 1.00 mg NaHCO3 10.00 g D-Fructose 10.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, fructose, vitamins, cysteine and sulfide, bring to the boil and cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add bicarbonate (solid) and equilibrate the medium with the gas until a pH of around 7.4 is reached. Then distribute and autoclave under the same gas atmosphere. Before use adjust the pH to 8.2 by adding a sterile anoxic stock solution of carbonate (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture (c. 0.25 ml per 10 ml medium) and add fructose, vitamins (sterilized by filtration), cysteine and sulfide from anoxic sterile stock solutions prepared under 100% N2. For autotrophic growth fructose is omitted and a gas atmosphere of 80% H2 and 20% CO2 is used. For DSM 1974 and DSM 7417 use only 1.00 g/l of NaHCO3 and omit Na2CO3 to reach a final pH of 6.5. For DSM 4132 use fructose as substrate at a final concentration of 1.00 g/l. © 2015 DSMZ GmbH - All rights reserved DSM 1974 is Moorella thermoautotrophica DSM 7417 is Moorella thermoautotrophica DSMZ Medium 135 DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium135.pdf 135. ACETOBACTERIUM MEDIUM NH4Cl 1.00 g KH2PO4 0.33 g K2HPO4 0.45 g MgSO4 x 7 H2O 0.10 g Trace element solution (see medium 141) 20.00 ml Yeast extract 2.00 g Resazurin 1.00 mg NaHCO3 10.00 g D-Fructose 10.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, fructose, vitamins, cysteine and sulfide, bring to the boil and cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add bicarbonate (solid) and equilibrate the medium with the gas until a pH of around 7.4 is reached. Then distribute and autoclave under the same gas atmosphere. Before use adjust the pH to 8.2 by adding a sterile anoxic stock solution of carbonate (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture (c. 0.25 ml per 10 ml medium) and add fructose, vitamins (sterilized by filtration), cysteine and sulfide from anoxic sterile stock solutions prepared under 100% N2. For autotrophic growth fructose is omitted and a gas atmosphere of 80% H2 and 20% CO2 is used. For DSM 1974 and DSM 7417 use only 1.00 g/l of NaHCO3 and omit Na2CO3 to reach a final pH of 6.5. For DSM 4132 use fructose as substrate at a final concentration of 1.00 g/l. © 2015 DSMZ GmbH - All rights reserved Carrine Blank Acetobacterium medium An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, magnesium sulfate, trace elements, yeast extract, resazurin, sodium bicarbonate, D-fructose, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. If fructose is not used, dihydrogen is used in the atmosphere. DSMZ Medium 133.3 Similar to DSMZ Medium 133, except para-aminobenzoic acid (4-aminobenzoic acid) is added. For chemoorganotrophic growth, sodium pyruvate and yeast extract are also added. Carrine Blank DSM 13294 is Tepidimonas DSMZ Medium 133.3 -< for DSM 13294 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium133.pdf 133. CARBON MONOXIDE OXIDIZER MEDIUM Na2HPO4 x 12 H2O 4.500 g KH2PO4 0.750 g NH4Cl 1.500 g MgSO4 x 7 H2O 0.200 g CaCl2 x 2 H2O 0.030 g Ferric ammonium citrate 0.018 g Trace element solution SL-6 (see medium 27) 1.000 ml Agar (if necessary) 12.000 g Distilled water 1000.000 ml Adjust pH to 7.0. For chemoautotrophic growth incubate under a gas atmosphere of a) 20 - 80% CO + 10% O2 + 70 - 10% N2 or b) 70% H2 + 20% O2 + 10% CO2 adding 2.5 g NaHCO3 per liter of medium. For chemoorganotrophic growth add 3.0 g sodium acetate and incubate under air. For DSM 1083 the medium has to be supplemented with 10 ml/l of the vitamin solution of medium 141, sterilized by filtration. For chemoorganotrophic growth with acetate under air add also 10 ml/l of a 5% w/v NaHCO3 solution, sterilized by filtration. For DSM 1085 the medium has to be supplemented with 20 μg/l vitamin B12. For chemoorganotrophic growth with acetate under air add also 20 ml/l of a 5% w/v NaHCO3 solution, sterilized by filtration. For DSM 13294 the medium has to be supplemented with 50 μg/l paraaminobenzoic acid. For chemoorganotrophic growth under air add also 2 g/l Na-pyruvate and 1 g/l yeast extract. © 2009 DSMZ GmbH - All rights reserved DSMZ Medium 133.2 DSMZ Medium 133.2 -< for DSM 1085 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium133.pdf 133. CARBON MONOXIDE OXIDIZER MEDIUM Na2HPO4 x 12 H2O 4.500 g KH2PO4 0.750 g NH4Cl 1.500 g MgSO4 x 7 H2O 0.200 g CaCl2 x 2 H2O 0.030 g Ferric ammonium citrate 0.018 g Trace element solution SL-6 (see medium 27) 1.000 ml Agar (if necessary) 12.000 g Distilled water 1000.000 ml Adjust pH to 7.0. For chemoautotrophic growth incubate under a gas atmosphere of a) 20 - 80% CO + 10% O2 + 70 - 10% N2 or b) 70% H2 + 20% O2 + 10% CO2 adding 2.5 g NaHCO3 per liter of medium. For chemoorganotrophic growth add 3.0 g sodium acetate and incubate under air. For DSM 1083 the medium has to be supplemented with 10 ml/l of the vitamin solution of medium 141, sterilized by filtration. For chemoorganotrophic growth with acetate under air add also 10 ml/l of a 5% w/v NaHCO3 solution, sterilized by filtration. For DSM 1085 the medium has to be supplemented with 20 μg/l vitamin B12. For chemoorganotrophic growth with acetate under air add also 20 ml/l of a 5% w/v NaHCO3 solution, sterilized by filtration. For DSM 13294 the medium has to be supplemented with 50 μg/l paraaminobenzoic acid. For chemoorganotrophic growth under air add also 2 g/l Na-pyruvate and 1 g/l yeast extract. © 2009 DSMZ GmbH - All rights reserved DSM 1085 is Methyloversatilis universalis Similar to DSMZ Medium 133, except vitamin B23 (cobalamin) is added. For chemoorganotrophic growth, sodium bicarbonate is also added. Carrine Blank DSMZ Medium 133.1 Similar to DSMZ Medium 133, except vitamins are added. If grown under chemoorganotrophic growth, sodium bicarbonate is added. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium133.pdf 133. CARBON MONOXIDE OXIDIZER MEDIUM Na2HPO4 x 12 H2O 4.500 g KH2PO4 0.750 g NH4Cl 1.500 g MgSO4 x 7 H2O 0.200 g CaCl2 x 2 H2O 0.030 g Ferric ammonium citrate 0.018 g Trace element solution SL-6 (see medium 27) 1.000 ml Agar (if necessary) 12.000 g Distilled water 1000.000 ml Adjust pH to 7.0. For chemoautotrophic growth incubate under a gas atmosphere of a) 20 - 80% CO + 10% O2 + 70 - 10% N2 or b) 70% H2 + 20% O2 + 10% CO2 adding 2.5 g NaHCO3 per liter of medium. For chemoorganotrophic growth add 3.0 g sodium acetate and incubate under air. For DSM 1083 the medium has to be supplemented with 10 ml/l of the vitamin solution of medium 141, sterilized by filtration. For chemoorganotrophic growth with acetate under air add also 10 ml/l of a 5% w/v NaHCO3 solution, sterilized by filtration. For DSM 1085 the medium has to be supplemented with 20 μg/l vitamin B12. For chemoorganotrophic growth with acetate under air add also 20 ml/l of a 5% w/v NaHCO3 solution, sterilized by filtration. For DSM 13294 the medium has to be supplemented with 50 μg/l paraaminobenzoic acid. For chemoorganotrophic growth under air add also 2 g/l Na-pyruvate and 1 g/l yeast extract. © 2009 DSMZ GmbH - All rights reserved DSM 1083 is Pseudomonas carboxydohydrogena DSMZ Medium 133.1 -< for DSM 1083 DSMZ Medium 133 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium133.pdf 133. CARBON MONOXIDE OXIDIZER MEDIUM Na2HPO4 x 12 H2O 4.500 g KH2PO4 0.750 g NH4Cl 1.500 g MgSO4 x 7 H2O 0.200 g CaCl2 x 2 H2O 0.030 g Ferric ammonium citrate 0.018 g Trace element solution SL-6 (see medium 27) 1.000 ml Agar (if necessary) 12.000 g Distilled water 1000.000 ml Adjust pH to 7.0. For chemoautotrophic growth incubate under a gas atmosphere of a) 20 - 80% CO + 10% O2 + 70 - 10% N2 or b) 70% H2 + 20% O2 + 10% CO2 adding 2.5 g NaHCO3 per liter of medium. For chemoorganotrophic growth add 3.0 g sodium acetate and incubate under air. For DSM 1083 the medium has to be supplemented with 10 ml/l of the vitamin solution of medium 141, sterilized by filtration. For chemoorganotrophic growth with acetate under air add also 10 ml/l of a 5% w/v NaHCO3 solution, sterilized by filtration. For DSM 1085 the medium has to be supplemented with 20 μg/l vitamin B12. For chemoorganotrophic growth with acetate under air add also 20 ml/l of a 5% w/v NaHCO3 solution, sterilized by filtration. For DSM 13294 the medium has to be supplemented with 50 μg/l paraaminobenzoic acid. For chemoorganotrophic growth under air add also 2 g/l Na-pyruvate and 1 g/l yeast extract. © 2009 DSMZ GmbH - All rights reserved DSM strains: A minerals-salts, solid culture medium comprised of sodium phosphate, potassium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, ferric ammonium citrate, trace elements, and agar. Prepared under an atmosphere of carbon monoxide, dioxygen, and dinitrogen. Or prepared under an atmosphere of dihydrogen, dioxygen, and carbon dioxide, with sodium bicarbonate. Or, sodium acetate is added and is prepared under an atmosphere of air (dioxygen). carbon monoxide oxidizer medium Carrine Blank DSMZ Medium 134 Carrine Blank DSM strains: An organic-rich, liquid culture medium comprised of potassium phosphate, sodium phosphate, calcium chloride, magnesium sulfate, ferric trichloride, manganese sulfate, trace elements, glutamic acid, trypticase soy broth without glucose (An organic-rich, liquid culture medium comprised of pancreatic digest of casein (casitone), papaic digest of soybean (soy peptone), sodium chloride, and dipotassium phosphate (potassium dibasic phosphate)), thamine hydrochloride, vitamin B12 (cobalamin), and glucose. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium134.pdf 134. HALISCOMENOBACTER MEDIUM KH2PO4 27.00 mg K2HPO4 40.00 mg Na2HPO4 x 2 H2O 40.00 mg CaCl2 x 2 H2O 50.00 mg MgSO4 x 7 H2O 75.00 mg FeCl3 x 6 H2O 5.00 mg MnSO4 x H2O 3.00 mg Trace element solution SL-6 (see medium 27) 1.00 ml Glutamic acid 1.31 g Trypticase soy broth without glucose (BBL) 2.50 mg Thiamine-HCl x 2 H2O 0.40 mg Vitamin B12 0.01 mg Glucose 2.00 g Distilled water 1000.00 ml Adjust pH to 7.5. The vitamins are sterilized by filtration. The glucose is dissolved in 5 ml of distilled water and sterilized separately by autoclaving. © 2007 DSMZ GmbH - All rights reserved Haliscomenobacter medium potato infusion 129. POTATO DEXTROSE AGAR Infusion from potatoes (see below) 1000.0 ml Glucose 20.0 g Agar 15.0 g Potato infusion: Boil 200 g scrubbed and sliced potatoes in 1000 ml water for 1 hour. Pass through fine sieve. Avoid new potatoes. © 2007 DSMZ GmbH - All rights reserved Carrine Blank A water extract of potatoes (Solanum tuberosum), prepared by boiling then filtering the aqueous extract. DSMZ Medium 129 DSM strains: potato dextrose agar http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium129.pdf 129. POTATO DEXTROSE AGAR Infusion from potatoes (see below) 1000.0 ml Glucose 20.0 g Agar 15.0 g Potato infusion: Boil 200 g scrubbed and sliced potatoes in 1000 ml water for 1 hour. Pass through fine sieve. Avoid new potatoes. © 2007 DSMZ GmbH - All rights reserved Carrine Blank An organic-rich, solid culture medium comprised of potato infusion, glucose, and agar. DSMZ Medium 130 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium130.pdf 130. CZAPEK-DOX AGAR Sucrose 30.00 g NaNO3 3.00 g MgSO4 x 7 H2O 0.50 g KCl 0.50 g FeSO4 x 7 H2O 0.01 g K2HPO4 1.00 g Agar 13.00 g Distilled water 1000.00 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: An organic-rich, solid culture medium comprised of sucrose, sodium nitrate, magnesium sulfate, potassium chloride, ferrous sulfate, potassium phosphate, and agar. Czapek-Dox agar DSMZ Medium 127 An organic-rich, solid culture medium comprised of glucose, soluble starch, yeast extract, casitone, calcium carbonate, and agar. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium127.pdf 127. MICROMONOSPORA MEGALOMICEA MEDIUM Glucose 10.0 g Soluble starch 20.0 g Yeast extract 5.0 g Casitone 5.0 g CaCO3 1.0 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved Carrine Blank Micromonospora megalomicea medium DSM strains: DSMZ Medium 126 Similar to DSMZ Medium 1, except glycerol is added. Mycobacterium intracellulare medium Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium126.pdf 126. MYCOBACTERIUM INTRACELLULARE MEDIUM To medium 1 add 20 g glycerol. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 124.2 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium124.pdf 124. DESULFOTOMACULUM ACETOXIDANS MEDIUM NaCl 1.17 g MgCl2 x 6 H2O 0.40 g KCl 0.30 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.27 g KH2PO4 0.20 g Na2SO4 2.84 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Na-acetate 1.40 g Na-butyrate 1.40 g Yeast extract 1.00 g Resazurin 0.50 mg NaHCO3 4.50 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.40 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% N2 and 20% CO2 gas atmosphere and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under N2. The vitamin solution should be sterilized by filtration. Prior to use check pH of completed medium and adjust to 7.0 - 7.2, if necessary. For DSM 2925 replace acetate and butyrate with 1.00 g/l ethanol added from a sterile anoxic stock solution after autoclaving. For DSM 7213 replace acetate and butyrate with 0.60 g/l Na-benzoate added from a sterile anoxic stock solution after autoclaving. © 2014 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 124, except sodium acetate and sodium butyrate are omitted and sodium benzoate is added. DSM 7213 is Desulfotomaculum gibsoniae DSM 7213 DSMZ Medium 124.2 -< for DSM 7213 DSMZ Medium 124.1 Similar to DSMZ Medium 124, except sodium acetate and sodium butyrate are omitted and ethanol is added. DSMZ Medium 124.1 -< for DSM 2925 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium124.pdf 124. DESULFOTOMACULUM ACETOXIDANS MEDIUM NaCl 1.17 g MgCl2 x 6 H2O 0.40 g KCl 0.30 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.27 g KH2PO4 0.20 g Na2SO4 2.84 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Na-acetate 1.40 g Na-butyrate 1.40 g Yeast extract 1.00 g Resazurin 0.50 mg NaHCO3 4.50 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.40 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% N2 and 20% CO2 gas atmosphere and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under N2. The vitamin solution should be sterilized by filtration. Prior to use check pH of completed medium and adjust to 7.0 - 7.2, if necessary. For DSM 2925 replace acetate and butyrate with 1.00 g/l ethanol added from a sterile anoxic stock solution after autoclaving. For DSM 7213 replace acetate and butyrate with 0.60 g/l Na-benzoate added from a sterile anoxic stock solution after autoclaving. © 2014 DSMZ GmbH - All rights reserved DSM 2925 is Acetobacterium carbinolicum Carrine Blank DSMZ Medium 124 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium124.pdf 124. DESULFOTOMACULUM ACETOXIDANS MEDIUM NaCl 1.17 g MgCl2 x 6 H2O 0.40 g KCl 0.30 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.27 g KH2PO4 0.20 g Na2SO4 2.84 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Na-acetate 1.40 g Na-butyrate 1.40 g Yeast extract 1.00 g Resazurin 0.50 mg NaHCO3 4.50 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.40 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% N2 and 20% CO2 gas atmosphere and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under N2. The vitamin solution should be sterilized by filtration. Prior to use check pH of completed medium and adjust to 7.0 - 7.2, if necessary. For DSM 2925 replace acetate and butyrate with 1.00 g/l ethanol added from a sterile anoxic stock solution after autoclaving. For DSM 7213 replace acetate and butyrate with 0.60 g/l Na-benzoate added from a sterile anoxic stock solution after autoclaving. © 2014 DSMZ GmbH - All rights reserved Desulotomaculum acetoxidans medium An organic-rich, liquid culture medium comprised of sodium chloride, magnesium chloride, potassium chloride, calcium chloride, ammonium chloride, potassium phosphate, sodium sulfate, trace elements, selenite-tungstate, sodium acetate, sodium butyrate, yeast extract, resazurin, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: Carrine Blank DSMZ Medium 125 A minerals-salts, solid culture medium comprised of potassium nitrate, magnesium sulfate, calcium chloride, sodium phosphate, ferrous sulfate, copper sulfate, boric acid, manganese sulfate, zinc sulfate, molybdenum trioxide, and agar. Prepared with methanol or an atmosphere of methane and air (dioxygen). http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium125.pdf 125. METHYLOBACTERIUM MEDIUM KNO3 1.00 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.02 g Na2HPO4 0.23 g NaH2PO4 0.07 g FeSO4 x 7 H2O 1.00 mg CuSO4 x 5 H2O 5.00 μg H3BO3 10.00 μg MnSO4 x 5 H2O 10.00 μg ZnSO4 x 7 H2O 70.00 μg MoO3 10.00 μg Agar 12.00 g Distilled water 1000.00 ml Add 5 ml methanol or incubate under methane-air (4:1). Adjust pH to 6.8. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Methylobacterium medium DSM strains: DSMZ Medium 123 Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium123.pdf 123. MARINE AGAR To 1000 ml "Synthetic sea water" (see medium 79) add 5 g Bacto Peptone, 1 g Bacto Yeast extract, and 15 g agar. Alternatively medium 514 may be used. © 2007 DSMZ GmbH - All rights reserved marine agar An organic-rich, solid culture medium comprised of synthetic sea water, peptone, yeast extract, and agar. DSMZ Medium 121 Carrine Blank Medium for Campylobacter DSM 806 DSM strains: An organic-rich, liquid culture medium comprised of sodium aspartate, yeast extract, magnesium sulfate, calcium chloride, potassium phosphate, sodium phosphate, cysteine hydrochloride, resazurin, and trace elements. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium121.pdf 121. MEDIUM FOR CAMPYLOBACTER DSM 806 Na-aspartate 10.00 g Yeast extract 0.20 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 28.00 mg K2HPO4 0.75 g NaH2PO4 0.25 g Cysteine-HCl x H2O 0.25 g Resazurin 1.00 mg Trace element solution 1.00 ml SL-10 (see medium 320) Distilled water 1000.00 ml Adjust pH to 7.0. Phosphate and cysteine should be dissolved together in 1/10 vol. of water and sterilized separately. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 122 An organic-rich, liquid culture medium comprised of ammonium sulfate, magnesium chloride, potassium phosphate, calcium chloride, sodium beta-glycerol phosphate (sodium glycerol-2-phosphate), ferrous sulfate, L-glutathione (glutathione), yeast extract, resazuring, and cellulose or cellobiose. Prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium122.pdf 122. CLOSTRIDIUM THERMOCELLUM MEDIUM (NH4)2SO4 1.30 g MgCl2 x 6 H2O 2.60 g KH2PO4 1.43 g K2HPO4 5.50 g CaCl2 x 2 H2O 0.13 g Na2-ß-glycerol phosphate x 4 H2O 6.00 g FeSO4 x 7 H2O 1.10 mg L-Glutathione reduced 0.25 g Yeast extract 4.50 g Resazurin 1.00 mg Cellulose (Avicel or MN 300) 10.00 g or Cellobiose 5.00 g Distilled water 1000.00 ml Prepare medium under 80% N2 and 20% CO2 gas atmosphere. Adjust pH to 7.0 - 7.2 and autoclave. Cellobiose is added after autoclaving from an anoxic and filter-sterilized stock solution of 10% (w/v). A white precipitate forms after mixing the ingredients of this medium, but this has no negative effect on growth. © 2009 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: Clostridium thermocellum medium DSMZ Medium 120b.1 DSM 22503 is Methanosarcina subterranea http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120b.pdf 120b. METHANOMICROCOCCUS MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Na-acetate 2.50 g Resazurin 0.50 mg NaHCO3 2.50 g Coenzyme M (2-mercaptoethanesulfonate) 0.14 g Vitamin solution (see medium 141) 10.00 ml Methanol 10.00 ml L-Cysteine-HCl x H2O 0.36 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, coenzyme M, vitamins, methanol, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% H2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 7.2 and dispense medium under 80% H2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins and coenzyme M are prepared under 100% N2 and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 7.0 – 7.2. After inoculation, pressurize the gas head space of culture bottles with sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. For DSM 22503 supplement medium with 6.0 g/l NaCl. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 120b.1 -< for DSM 22503 Similar to DSMZ Medium 120b, except the concentration of sodium chloride is increased. Carrine Blank DSMZ Medium 120b A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, yeast extract, casitone, sodium acetate, resazurin, sodium bicarbonate, vitamins, methanol, L-cysteine hydrochloride, coenzyme M, and sodium sulfide. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide. Carrine Blank DSM strains: Methanomicrococcus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120b.pdf 120b. METHANOMICROCOCCUS MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Na-acetate 2.50 g Resazurin 0.50 mg NaHCO3 2.50 g Coenzyme M (2-mercaptoethanesulfonate) 0.14 g Vitamin solution (see medium 141) 10.00 ml Methanol 10.00 ml L-Cysteine-HCl x H2O 0.36 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, coenzyme M, vitamins, methanol, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% H2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 7.2 and dispense medium under 80% H2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins and coenzyme M are prepared under 100% N2 and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 7.0 – 7.2. After inoculation, pressurize the gas head space of culture bottles with sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. For DSM 22503 supplement medium with 6.0 g/l NaCl. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 120c Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120c.pdf 120c. GÖ1 MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Na-acetate 10.00 g Sucrose 17.10 g Resazurin 0.50 mg NaHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins are prepared under 100% N2 and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 6.8 – 7.0. © 2014 DSMZ GmbH - All rights reserved Goe1 medium A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, yeast extract, casitone, sodium acetate, resazurin, sodium bicarbonate, vitamins, methanol, L-cysteine hydrochloride, sucrose, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. GÖ1 medium DSMZ Medium 120a.2 Carrine Blank DSMZ Medium 120a.2 -< for DSM 10334 and DSM 13486 Similar to DSMZ Medium 120a, except the concentration of sodium bicarbonate is increased and the concentration of methanol is decreased. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120a.pdf 120a. METHANOSARCINA BARKERI MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Resazurin 0.50 mg NaHCO3 0.85 g Vitamin solution (see medium 141) 10.00 ml Methanol 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins are prepared under 100% N2 gas and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 6.5 - 6.8. For DSM 4556 increase amount of NaHCO3 to 2.00 g/l to achieve a pH of 6.8 – 7.0 and add only 5.00 ml/l methanol. After inoculation pressurize vials with 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. For DSM 10334 and DSM 13486 increase amount of NaHCO3 to 2.00 g/l to achieve a pH of 6.8 – 7.0 and add only 5.00 ml/l methanol. © 2014 DSMZ GmbH - All rights reserved DSM 10334 is Methanosarcina lacustris Z-7289 DSM 13486 is Methanosarcina lacustris ZS DSMZ Medium 120a Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120a.pdf 120a. METHANOSARCINA BARKERI MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Resazurin 0.50 mg NaHCO3 0.85 g Vitamin solution (see medium 141) 10.00 ml Methanol 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins are prepared under 100% N2 gas and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 6.5 - 6.8. For DSM 4556 increase amount of NaHCO3 to 2.00 g/l to achieve a pH of 6.8 – 7.0 and add only 5.00 ml/l methanol. After inoculation pressurize vials with 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. For DSM 10334 and DSM 13486 increase amount of NaHCO3 to 2.00 g/l to achieve a pH of 6.8 – 7.0 and add only 5.00 ml/l methanol. © 2014 DSMZ GmbH - All rights reserved A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, yeast extract, casitone, resazurin, sodium bicarbonate, vitamins, methanol, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: Methanosarcina barkeri medium DSMZ Medium 120.3 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120.pdf 120. METHANOSARCINA MAZEI MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Na-acetate 2.50 g Resazurin 0.50 mg NaHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml Methanol 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins are prepared under 100% N2 and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 6.8 – 7.0. For DSM 1825, DSM 7081, and DSM 11855 supplement medium with 5% (v/v) rumen fluid, clarified (see medium 1310) and adjust pH to 6.5 – 6.8. For DSM 7058 and DSM 9195 replace methanol with 5.00 g/l trimethylamine-HCl. For DSM 21571 supplement medium with 6.00 g/l NaCl. © 2014 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 120, except the concentration of sodium chloride is increased. DSMZ Medium 120.3 -< for DSM 21571 DSM 21571 is Methanosarcina horonobensis DSMZ Medium 120.2 DSMZ Medium 120.2 -< for DSM 7058 and DSM 9195 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120.pdf 120. METHANOSARCINA MAZEI MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Na-acetate 2.50 g Resazurin 0.50 mg NaHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml Methanol 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins are prepared under 100% N2 and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 6.8 – 7.0. For DSM 1825, DSM 7081, and DSM 11855 supplement medium with 5% (v/v) rumen fluid, clarified (see medium 1310) and adjust pH to 6.5 – 6.8. For DSM 7058 and DSM 9195 replace methanol with 5.00 g/l trimethylamine-HCl. For DSM 21571 supplement medium with 6.00 g/l NaCl. © 2014 DSMZ GmbH - All rights reserved DSM 7058 is Methanosarcina mazei DSM 9195 is Methanosarcina mazei TMA Carrine Blank Similar to DSMZ Medium 120, except methanol is omitted and trimethylamine hydrochloride is added. DSMZ Medium 120.1 DSM 1825 is Methanosarcina thermophila TM-1 DSM 7081 is Methanosarcina thermophila DSM 11855 is Methanosarcina thermophila Similar to DSMZ Medium 120, except clarified rumen fluid is added, and the pH is decreased slightly to 6.5-6.8. DSMZ Medium 120.1 -< for DSM 1825, DSM 7081, and DSM 11855 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120.pdf 120. METHANOSARCINA MAZEI MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Na-acetate 2.50 g Resazurin 0.50 mg NaHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml Methanol 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins are prepared under 100% N2 and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 6.8 – 7.0. For DSM 1825, DSM 7081, and DSM 11855 supplement medium with 5% (v/v) rumen fluid, clarified (see medium 1310) and adjust pH to 6.5 – 6.8. For DSM 7058 and DSM 9195 replace methanol with 5.00 g/l trimethylamine-HCl. For DSM 21571 supplement medium with 6.00 g/l NaCl. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 120 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120.pdf 120. METHANOSARCINA MAZEI MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Na-acetate 2.50 g Resazurin 0.50 mg NaHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml Methanol 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins are prepared under 100% N2 and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 6.8 – 7.0. For DSM 1825, DSM 7081, and DSM 11855 supplement medium with 5% (v/v) rumen fluid, clarified (see medium 1310) and adjust pH to 6.5 – 6.8. For DSM 7058 and DSM 9195 replace methanol with 5.00 g/l trimethylamine-HCl. For DSM 21571 supplement medium with 6.00 g/l NaCl. © 2014 DSMZ GmbH - All rights reserved DSM strains: Methanosarcina mazei medium A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, yeast extract, casitone, sodium acetate, resazurin, sodium bicarbonate, vitamins, methanol, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. trace elements solution for DSMZ Medium 144 Carrine Blank A trace elements solution comprised of nitrilotriacetic acid, ferrous chloride, manganese chloride, cobalt chloride, calcium chloride, zinc chloride, copper chloride, boric acid, sodium molybdate, nickel chloride, sodium chloride, and sodium selenite. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium144.pdf 144. THERMOANAEROBIUM MEDIUM NH4Cl 0.90 g NaCl 0.90 g MgCl2 x 6 H2O 0.40 g KH2PO4 0.75 g K2HPO4 1.50 g Trace element solution (see below) 9.00 ml FeSO4 x 7 H2O 3.00 mg Yeast extract 3.00 g Tryptone or Trypticase 10.00 g Resazurin 0.50 mg Vitamin solution (see medium 141) 5.00 ml D-Glucose 5.00 g Na2S x 9 H2O 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except vitamins, glucose and sulfide), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere in culture vessels and autoclave. Add glucose, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under N2. Adjust pH of final medium to 7.2 - 7.4. For DSM 7040 omit tryptone and reduce amount of yeast extract to 1.00 g/l. For DSM 12299 omit D-glucose. Trace element solution: Nitrilotriacetic acid 12.80 g FeCl2 x 4 H2O 0.20 g MnCl2 x 4 H2O 0.10 g CoCl2 x 6 H2O 0.17 g CaCl2 x 2 H2O 0.10 g ZnCl2 0.10 g CuCl2 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 26.00 mg NaCl 1.00 g Na2SeO3 x 5 H2O 0.02 g Distilled water 1000.00 ml First, dissolve nitrilotriacetic acid and adjust to pH 6.5 with KOH. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 168 Spirochaeta aurantia medium Carrine Blank An organic-rich, liquid culture medium comprised of glucose, yeast extract, trypticase (trypticase peptone) agar, and potassium phosphate. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium168.pdf 168. SPIROCHAETA AURANTIA MEDIUM Solution A: Glucose 2.0 g Yeast extract 2.0 g Trypticase (BBL) 5.0 g Agar (Bacto, Difco) 10.0 g (if necessary) Distilled water 1000.0 ml Adjust pH to 7.5 with KOH. Solution B: 1 M K-phosphate-buffer, pH 7.0 10.0 ml Sterilize part A and B separately by autoclaving at 121˚C for 15 min and combine thereafter. Final pH: 7.0 - 7.3. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 146 DSM strains: Carrine Blank An organic-rich, solid culture medium comprised of skim milk (powder), magnesium sulfate, potassium nitrate, sodium chloride, ferric citrate, neopeptone, glycerol, and agar. Halococcus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium146.pdf 146. HALOCOCCUS MEDIUM Solution A: Skim milk (Difco) 50.0 g Distilled water 500.0 ml Solution B: MgSO4 x 7 H2O 10.0 g KNO3 2.0 g NaCl 200.0 g Fe-citrate trace - Distilled water 100.0 ml Solution C: Neopeptone 5.0 g Glycerol 10.0 g Agar 25.0 g Distilled water 400.0 ml Steam the three solutions separately, mix together solutions B and C and adjust pH to 8.4. Autoclave the skim milk at 112˚C for 15 min., and the agar salt mixture at 121˚C for 20 min. Add the hot mixture to the warm skim milk aseptically. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 147 Ruminobacter amylophilus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium147.pdf 147. RUMINOBACTER AMYLOPHILUS MEDIUM K2HPO4 0.45 g KH2PO4 0.45 g (NH4)2SO4 0.90 g NaCl 0.90 g MgSO4 x 7 H2O 0.18 g CaCl2 x 2 H2O 0.12 g Starch, soluble 5.00 g Casitone (Difco) 10.00 g Resazurin 1.00 mg Cysteine-HCl x H2O 0.50 g NaHCO3 6.00 g Distilled water 1000.00 ml Adjust pH to 7.0. Gas atmosphere: 100% CO2. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium sulfate, sodium chloride, magensium sulfate, calcium chloride, soluble starch, casitone, resazurin, cysteine hydrochloride, and sodium bicarbonate. Prepared under an atmosphere of carbon dioxide. DSMZ Medium 148 Desulfuromonas sp. medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium148.pdf 148. DESULFUROMONAS SP. MEDIUM KH2PO4 1.0 g NH4Cl 0.5 g MgSO4 x 7 H2O 0.4 g CaCl2 x 2 H2O 0.1 g Trace element solution SL-10 (see medium 320) 1.0 ml Selenite-tungstate solution (see medium 385) 1.0 ml Na-acetate 0.5 g Fumaric acid 1.5 g Resazurin 0.5 mg NaHCO3 2.5 g Vitamin solution (see medium 503) 1.0 ml Na2S x 9 H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except bicarbonate, vitamins, and sulfide), bring to the boil, then cool to room temperature under 100% N2 gas and dispense under same gas atmosphere in anoxic tubes or bottles. After autoclaving complete the medium by adding residual substances from separately autoclaved (vitamins are sterilized by filtration) anoxic stock solutions prepared under N2 gas or 80% N2 and 20% CO2 gas atmosphere (bicarbonate). Adjust pH of the completed medium to 7.0-7.2. © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of ptoassium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, trace elements, selenite-tungstate, sodium acetate, fumaric acid, resazurin, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: DSMZ Medium 149 Carrine Blank DSM strains: Desulfovibrio gigas medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium149.pdf 149. DESULFOVIBRIO GIGAS MEDIUM Solution A: KH2PO4 1.0 g NH4Cl 0.5 g MgSO4 x 7 H2O 0.4 g Na2SO4 2.0 g CaCl2 x 2 H2O 0.1 g Trace element solution SL-6 (see medium 27) 1.0 ml 2 M H2SO4 1.0 ml Na-L-lactate 2.0 g Distilled water 950.0 ml Solution B: NaHCO3 2.0 g Distilled water 40.0 ml Solution C: Vitamin solution (see medium 503) 1.0 ml Solution D: Na2S x 9 H2O 0.3 g Distilled water 10.0 ml Solution A is gassed with 100% N2 gas to make it anoxic (at least 30 min) and then autoclaved under the same gas mixture. Solution D is autoclaved separately under 100% N2 gas. Solution B is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under N2 gas atmosphere and sterilized by filtration. Solutions B to D are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.0 - 7.2. Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. © 2014 DSMZ GmbH - All rights reserved A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, sodium sulfate, calcium chloride, trace elements, sulfuric acid, sodium lactate, sodium bicarbonate, vitamins, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 150 Acidianus brierleyi medium Carrine Blank A minerals-salts, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, potassium chloride, calcium nitrate, yeast extract, elemental sulfur, and sulfuric acid. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium150.pdf 150. ACIDIANUS BRIERLEYI MEDIUM (NH4)2SO4 3.00 g K2HPO4 x 3 H2O 0.50 g MgSO4 x 7 H2O 0.50 g KCl 0.10 g Ca(NO3)2 0.01 g Yeast extract 0.20 g Sulfur (flowers) 10.00 g Distilled water 1000.00 ml Adjust pH with 6 N H2SO4 to 1.5 - 2.5. Yeast extract (10% w/v in distilled water) is autoclaved separately. Sulfur is sterilized by steaming for 3 hours on each of 3 successive days. For DSM 18786 adjust pH to 0.8. © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 150.1 DSM 18786 is Acidianus sulfidivorans JP7 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium150.pdf 150. ACIDIANUS BRIERLEYI MEDIUM (NH4)2SO4 3.00 g K2HPO4 x 3 H2O 0.50 g MgSO4 x 7 H2O 0.50 g KCl 0.10 g Ca(NO3)2 0.01 g Yeast extract 0.20 g Sulfur (flowers) 10.00 g Distilled water 1000.00 ml Adjust pH with 6 N H2SO4 to 1.5 - 2.5. Yeast extract (10% w/v in distilled water) is autoclaved separately. Sulfur is sterilized by steaming for 3 hours on each of 3 successive days. For DSM 18786 adjust pH to 0.8. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 150, except the pH is decreased to 0.8. DSMZ Medium 150.1 -< for DSM 18786 DSMZ Medium 150a A minerals-salts culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, potassium chloride, calcium nitrate, elemental sulfur, trace elements, and sulfuric acid. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium150a.pdf 150a. THIOBACILLUS CALDUS MEDIUM Use medium 150 without yeast extract. Adjust pH to 2.5 with sulfuric acid. After autoclaving add 10 ml/l of filter-sterilized trace elements solution (see below) and 5 g/l of sulfur powder (sterilized by steaming for 3 hours on each 3 successive days). Trace element solution: FeCl3 x 6 H2O 11.0 mg CuSO4 x 5 H2O 0.5 mg H3BO3 2.0 mg MnSO4 x H2O 2.0 mg Na2MoO4 x 2 H2O 0.8 mg CoCl2 x 6 H2O 0.6 mg ZnSO4 x 7 H2O 0.9 mg Distilled water 10.0 ml © 2010 DSMZ GmbH - All rights reserved Thiobacillus caldus medium Carrine Blank DSMZ Medium 153 Thioglycolate medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium153.pdf 153. THIOGLYCOLATE MEDIUM (MERCK 108190) Trypticase (BBL) 15.00 g L-Cystine 0.50 g Glucose 2.00 g Yeast extract 5.00 g NaCl 2.50 g Na-thioglycolate 0.50 g Resazurin 0.50 mg Distilled water 1000.00 ml Dissolve ingredients, sparge medium with 100% N2 gas for 30 – 45 min to make it anoxic. Adjust pH to 7.1 with Na2CO3 (10% w/v), if necessary. Then dispense medium in Hungate-type tubes under the same gas atmosphere and autoclave. For solid medium add 15.00 g/l agar prior to autoclaving. © 2014 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, liquid culture medium comprised of trypticase (trypticase peptone), L-cystine, glucose, yeast extract, sodium chloride, sodium thioglycolate, and resazurin. Prepared under an atmosphere of dinitrogen. Carrine Blank DSMZ Medium 155 Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium155.pdf 155. BEGGIATOA MEDIUM CaSO4 (saturated solution) 20.000 ml NH4Cl 0.450 mg K2HPO4 0.100 mg MgSO4 x 7 H2O 0.200 mg Trace element solution (see below) 5.000 ml Na-acetate 0.500 g Nutrient broth (Difco) 0.500 g Agar (if necessary) 10.000 g Catalase, filter-sterilized 15000 – 35000 units/1000 ml Distilled water 1000.000 ml Adjust pH to 7.4 using pH indicator paper before autoclaving. The catalase is added just before inoculation. Trace element solution: EDTA 0.200 g FeSO4 x 7 H2O 0.700 g ZnSO4 x 7 H2O 0.010 g MnSO4 x 4 H2O 0.002 g CuSO4 x 5 H2O 5.000 μg H3BO3 10.000 mg Co(NO3)2 1.000 mg Na2MoO4 x 2 H2O 1.000 mg Distilled water 1000.000 ml © 2009 DSMZ GmbH - All rights reserved Beggiatoa medium A minerals-salts, liquid culture medium comprised of calcium sulfate, ammonium chloride, potassium phosphate, magnesium sulfate, trace elements, sodium acetate, nutrient broth (peptone and beef extract), agar, and catalase. trace elements solution for DSMZ Medium 155 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium155.pdf 155. BEGGIATOA MEDIUM CaSO4 (saturated solution) 20.000 ml NH4Cl 0.450 mg K2HPO4 0.100 mg MgSO4 x 7 H2O 0.200 mg Trace element solution (see below) 5.000 ml Na-acetate 0.500 g Nutrient broth (Difco) 0.500 g Agar (if necessary) 10.000 g Catalase, filter-sterilized 15000 – 35000 units/1000 ml Distilled water 1000.000 ml Adjust pH to 7.4 using pH indicator paper before autoclaving. The catalase is added just before inoculation. Trace element solution: EDTA 0.200 g FeSO4 x 7 H2O 0.700 g ZnSO4 x 7 H2O 0.010 g MnSO4 x 4 H2O 0.002 g CuSO4 x 5 H2O 5.000 μg H3BO3 10.000 mg Co(NO3)2 1.000 mg Na2MoO4 x 2 H2O 1.000 mg Distilled water 1000.000 ml © 2009 DSMZ GmbH - All rights reserved A trace elements solution comprised of EDTA, ferrous suflate, zinc sulfate, manganese sulfate, copper sulfate, boric acid, cobalt nitrate, and sodium molybdate. DSMZ Medium 156 An organic-rich, liquid culture medium comprised of L-alanine, peptone, yeast extract, cysteine hydrochloride, magnesium sulfate, ferrous sulfate, potassium phosphate, calcium sulfate, resazurin, and sodium bicarbonate. Prepared under an atmosphere of dinitrogen. DSM strains: Clostridium propionicum medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium156.pdf 156. CLOSTRIDIUM PROPIONICUM MEDIUM L-Alanine 3.0 g Peptone 3.0 g Yeast extract 4.0 g Cysteine-HCl x H2O 0.3 g MgSO4 x 7 H2O 0.1 g FeSO4 x 7 H2O 18.0 mg Potassium phosphate buffer (1 M, pH 7.1) 5.0 ml CaSO4 (satur. sol.) 2.5 ml Resazurin 1.0 mg NaHCO3 1.0 g Distilled water 1000.0 ml Dissolve ingredients (except bicarbonate), adjust pH to 7.0, then sparge medium with 100% N2 gas to make it anoxic (ca. 30 – 45 min). Add and dissolve 1.0 g/l NaHCO3 to the medium, then dispense under N2 gas atmosphere in culture vessels and autoclave. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 158 Thermoplasma acidophilum medium DSM strains: An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium sulfate, magnesium sulfate, calcium chloride, trace elements, yeast extract, glucose, and sulfuric acid. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium158.pdf 158. THERMOPLASMA ACIDOPHILUM MEDIUM (NH4)2SO4 1.320 g KH2PO4 0.372 g MgSO4 x 7 H2O 0.247 g CaCl2 x 2 H2O 0.074 g Trace element solution (see below) 10.000 ml Yeast extract (Difco) 2.000 g Glucose 20.000 g Distilled water 1000.000 ml (freshly distilled) Adjust pH to 1.0 with 1 N H2SO4. Stock solutions of yeast extract (10% w/v) and glucose (50% w/v) in water are sterilized separately by autoclaving and thereafter added to the sterile mineral salt medium. Trace element solution: FeCl3 x 6 H2O 1.930 g MnCl2 x 4 H2O 0.180 g Na2B4O7 x 10 H2O 0.450 g ZnSO4 x 7 H2O 22.000 mg CuCl2 x 2 H2O 5.000 mg Na2MoO4 x 2 H2O 3.000 mg VOSO4 x 5 H2O 3.800 mg CoSO4 x 7 H2O 2.000 mg Distilled water 1000.000 ml © 2007 DSMZ GmbH - All rights reserved trace elements solution for DSMZ Medium 158 Carrine Blank A trace elements solution comprised of ferric trichloride, manganese chloride, sodium tetraborate, zinc sulfate, copper chloride, sodium molybdate, vanadyl sulfate, and cobalt sulfate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium158.pdf 158. THERMOPLASMA ACIDOPHILUM MEDIUM (NH4)2SO4 1.320 g KH2PO4 0.372 g MgSO4 x 7 H2O 0.247 g CaCl2 x 2 H2O 0.074 g Trace element solution (see below) 10.000 ml Yeast extract (Difco) 2.000 g Glucose 20.000 g Distilled water 1000.000 ml (freshly distilled) Adjust pH to 1.0 with 1 N H2SO4. Stock solutions of yeast extract (10% w/v) and glucose (50% w/v) in water are sterilized separately by autoclaving and thereafter added to the sterile mineral salt medium. Trace element solution: FeCl3 x 6 H2O 1.930 g MnCl2 x 4 H2O 0.180 g Na2B4O7 x 10 H2O 0.450 g ZnSO4 x 7 H2O 22.000 mg CuCl2 x 2 H2O 5.000 mg Na2MoO4 x 2 H2O 3.000 mg VOSO4 x 5 H2O 3.800 mg CoSO4 x 7 H2O 2.000 mg Distilled water 1000.000 ml © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 157 DSM strains: An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium phosphate, fumaric acid, sodium formate, yeast extract, manganese chloride, ferrous sulfate, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium157.pdf 157. WOLINELLA SUCCINOGENES MEDIUM (NH4)2SO4 1.00 g K2HPO4 5.00 g Fumaric acid 3.00 g Na-formate 3.00 g Yeast extract 1.00 g MgCl2 x 6 H2O 0.20 g FeSO4 x 7 H2O 0.02 g Resazurin 0.50 mg L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except cysteine and sulfide), boil for 1 min, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in anoxic tubes or bottles and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2 and adjust pH of completed medium to 7.0 - 7.2. © 2015 DSMZ GmbH - All rights reserved Carrine Blank Wolinella succinogenes medium DSMZ Medium 160 An organic-rich, solid culture medium containing casitone, yeast extract, cellobiose (or filter paper) and calcium chloride. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium160.pdf 160. CYTOPHAGA HUTCHINSONII MEDIUM 1. To medium 67 add 0.5% cellobiose (filter-sterilized) or 2. Distribute medium 67 (without agar) in 5 ml amounts in test tubes, add a strip of filter paper (7 cm long) to each and autoclave, or place sterile pieces of filter paper on the surface of agarized medium 67. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Cytophaga hutchinsonii medium DSM strains: DSMZ Medium 159 Carrine Blank DSM strains: Treponema bryantii medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium159.pdf 159. TREPONEMA BRYANTII MEDIUM Rumen fluid, clarified 300.000 ml CaCl2 x 2 H2O 0.120 g MgSO4 x 7 H2O 0.180 g KH2PO4 0.450 g K2HPO4 0.450 g NaCl 0.900 g (NH4)2SO4 0.900 g Resazurin 0.001 g Cysteine-HCl x H2O 1.000 g Distilled water 580.000 ml Adjust pH to 7.0 with KOH NaHCO3 solution (5% w/v) 100.000 ml Glucose solution (10% w/v) 20.000 ml The medium is prepared under 100% CO2 atmosphere. Sodium bicarbonate and glucose solution are filter-sterilized and added to the main part of the medium after it has been autoclaved and cooled to room temperature. Final pH should be 6.7 - 7.0. © 2007 DSMZ GmbH - All rights reserved A minerals-salts, liquid culture medium comprised of clarified rumen fluid, calcium chloride, magnesium sulfate, potassium phosphate, sodium chloride, ammonium sulfate, resazurin, cysteine hydrochloride, sodium bicarbonate, and glucose. Prepared under an atmosphere of carbon dioxide. DSMZ Medium 161 Carrine Blank An organic-rich, liquid culture medium comprised of clarified rumen fluid, potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, trace elements, ferrous sulfate, yeast extract, trypticase (trypticase peptone), fatty acid mixture, resazurin, vitamins, L-cysteine hydrochloride, and sodium sulfide. Methanomicrobium mobile medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium161.pdf 161. METHANOMICROBIUM MOBILE MEDIUM Rumen fluid, clarified (see medium 1310) 300.00 ml K2HPO4 0.30 g KH2PO4 0.30 g (NH4)2SO4 0.30 g NaCl 0.60 g MgSO4 x 7 H2O 0.13 g CaCl2 x 2 H2O 8.00 mg Trace elements (see medium 141) 10.00 ml FeSO4 x 7 H2O 2.00 mg Yeast extract (Difco) 1.00 g Trypticase (BBL) 1.00 g Fatty acid mixture (see medium 119) 20.00 ml Resazurin 0.50 mg NaHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 660.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% H2 and 20% CO2 gas mixture to make it anoxic. Add and dissolve bicarbonate and adjust pH to 6.8, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. Adjust pH of final medium to 6.5. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 163 Desulfovibrio medium DSM strains: Desulfovibrio medium (marine) Carrine Blank Desulfovibrio marine medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium163.pdf 163. DESULFOVIBRIO MEDIUM (MARINE) Solution A: NaCl 25.0 g K2HPO4 0.5 g NH4Cl 1.0 g Na2SO4 1.0 g CaCl2 x 2 H2O 0.1 g MgSO4 x 7 H2O 2.0 g Na-DL-lactate 2.0 g Yeast extract 1.0 g Resazurin 1.0 mg Distilled water 980.0 ml Solution B: FeSO4 x 7 H2O 0.5 g Distilled water 10.0 ml Solution C: Na-thioglycolate 0.1 g Ascorbic acid 0.1 g Distilled water 10.0 ml Bring solution A to the boil, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121˚C. For DSM 10520 adjust final pH of autoclaved medium to 7.5 using a sterile anoxic stock solution of NaHCO3 (10% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere. © 2015 DSMZ GmbH - All rights reserved A minerals-salts, liquid culture medium comprised of sodium chloride, potassium phosphate, ammonium chloride, sodium sulfate, calcium chloride, magnesium sulfate, sodium lactate, yeast extract, resazurin, ferrous sulfate, sodium thioglycolate, and ascorbic acid. Prepared under an atmosphere of dinitrogen. DSMZ Medium 163.1 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium163.pdf 163. DESULFOVIBRIO MEDIUM (MARINE) Solution A: NaCl 25.0 g K2HPO4 0.5 g NH4Cl 1.0 g Na2SO4 1.0 g CaCl2 x 2 H2O 0.1 g MgSO4 x 7 H2O 2.0 g Na-DL-lactate 2.0 g Yeast extract 1.0 g Resazurin 1.0 mg Distilled water 980.0 ml Solution B: FeSO4 x 7 H2O 0.5 g Distilled water 10.0 ml Solution C: Na-thioglycolate 0.1 g Ascorbic acid 0.1 g Distilled water 10.0 ml Bring solution A to the boil, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121˚C. For DSM 10520 adjust final pH of autoclaved medium to 7.5 using a sterile anoxic stock solution of NaHCO3 (10% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 163, except the final pH is reduced to 7.5 and sodium bicarbonate is added. Also prepared under an atmosphere of dinitrogen and carbon dioxide. DSM 10520 is Desulfovibrio vietnamensis DSM 10520 DSMZ Medium 163.1 -< for DSM 10520 Carrine Blank DSMZ Medium 162 DSM strains: An organic-rich, liquid culture medium comprised of potassium phosphate, sodium phosphate, ammonium sulfate, magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, sodium molybdate, methylamine hydrochloride, and yeast extract. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium162.pdf 162. HYPHOMICROBIUM MEDIUM KH2PO4 1.36 g Na2HPO4 2.13 g (NH4)2SO4 0.50 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 6.00 mg FeSO4 x 7 H2O 3.00 mg MnSO4 x H2O 1.00 mg Na2MoO4 x 2 H2O 1.50 mg Methylamine hydrochloride 3.38 g Yeast extract 0.10 g Distilled water 1000.00 ml Adjust pH to 7.4 with NaOH. Autoclave 20 min at 121˚C; final pH: 7.2. © 2007 DSMZ GmbH - All rights reserved Hyphomicrobium medium Carrine Blank DSMZ Medium 165 Acetivibrio cellulolyticus medium An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, ferrous sulfate, vitamins, trace elements, cellobiose (or cellulose), resazurin, sodium bicarbonate, cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium165.pdf 165. ACETIVIBRIO CELLULOLYTICUS MEDIUM Mineral solution 1 (see below) 75.000 ml Mineral solution 2 (see below) 75.000 ml FeSO4 x 7 H2O 0.020 g Vitamin solution (see medium 141) 10.000 ml Trace element solution (see medium 141) 10.000 ml Cellobiose or cellulose 3.000 g (MN 300, Whatman CF II, Kleenex tissue paper, or HCl treated cotton) Resazurin 0.001 g NaHCO3 2.000 g Cysteine-HCl x H2O 0.250 g Na2S x 9 H2O 0.250 g Distilled water 830.000 ml Adjust pH to 7.6. Gas atmosphere: 80% N2 + 20% CO2. Final pH after autoclaving is 7.2. Cellobiose is filter-sterilized separately, flushed and stored under nitrogen gas until needed. Cysteine and sodium sulfide are each autoclaved separately in concentrated solutions under nitrogen gas. Mineral solution 1: K2HPO4 3.900 g Distilled water 1000.000 ml Mineral solution 2: KH2PO4 2.400 g (NH4)2SO4 6.000 g NaCl 0.590 g MgSO4 x 7 H2O 1.200 g CaCl2 x 2 H2O 0.720 g Distilled water 1000.000 ml © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank DSMZ Medium 166 Carrine Blank DSM strains: An organic-rich, solid culture medium comprised of ammonium sulfate, magnesium sulfate, sodium phosphate, potassium phosphate, trace elements, methylamine hydrochloride, and agar. Hyphomicrobium strain X medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium166.pdf 166. HYPHOMICROBIUM STRAIN X MEDIUM (NH4)2SO4 1.00 g MgSO4 x 7 H2O 0.20 g NaH2PO4 x H2O 0.50 g K2HPO4 1.55 g Trace element solution 0.20 ml (see medium 69) Methylamine hydrochloride 3.40 g Agar 15.00 g Distilled water 1000.00 ml Adjust pH to 7.2. For liquid medium use only 0.7 g of methylamine hydrochloride per liter. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 169 Spirochaeta zuelzerae medium Carrine Blank DSM strains: An organic-rich, liquid culture medium comprised of glucose, yeast extract, calcium chloride, magnesium sulfate, cysteine hydrochloride, agar, sodium bicarbonate, and potassium phosphate. Prepared under an atmosphere of dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium169.pdf 169. SPIROCHAETA ZUELZERAE MEDIUM Solution A: Glucose 2.00 g Yeast extract (Difco) 4.00 g CaCl2 x 2 H2O 0.04 g MgSO4 x 7 H2O 0.50 g Cysteine-HCl x H2O 0.50 g Agar (Bacto, Difco) (if necessary) 10.00 g Distilled water 960.00 ml Adjust pH to 7.2 with KOH. Solution B: NaHCO3 1.00 g Distilled water 20.00 ml Solution C: 0.5 M K-phosphate buffer, pH 7.4 20.0 ml Solution A (without cysteine) is boiled for few minutes to remove dissolved oxygen, and cooled to room temperature under a stream of 100% nitrogen gas. Then cysteine is added and the pH adjusted to 7.2. The three parts are sterilized separately each by autoclaving under 100% nitrogen atmosphere and combined thereafter. Final pH of the complete medium is 7.5. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 170 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium170.pdf 170. YEAST EXTRACT - SKIM MILK MEDIUM Skim milk (Difco) 10.0 g Yeast extract 1.0 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank An organic-rich, solid culture medium comprised of skim milk powder, yeast extract, and agar. yeast extract-skim milk medium Skim milk powder rehydrated at a 10% solution is pH 6.3 and Bacto Yeast Extract in solution is pH 6.3-7.3. One cup contains 237 mL; 1 cup of skim milk contains 127 mg sodium and 407 mg potassium (=0.534g/237mL = 0.2% due to cations, assume 0.4% due to cations and anions). DSMZ Medium 171 A minerals-salts, liquid culture medium comprised of potassium phosphate, sodium phosphate, ammonium chloride, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, ferrous sulfate, trace elements, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium171.pdf 171. ACETOGENIUM MEDIUM K2HPO4 0.220 g KH2PO4 0.220 g NaH2PO4 x H2O 4.500 g Na2HPO4 x 12 H2O 6.100 g NH4Cl 0.310 g (NH4)2SO4 0.220 g NaCl 0.450 g MgSO4 x 7 H2O 0.090 g CaCl2 x 2 H2O 0.006 g FeSO4 x 7 H2O 0.002 g Trace element solution (see medium 141) 10.000 ml Resazurin 0.001 g L-Cysteine-HCl x H2O 0.500 g Na2S x 9 H2O 0.500 g Distilled water 1000.000 ml Adjust final pH to 6.5. Use 80% H2 + 20% CO2 mixture as gas atmosphere at one atmosphere overpressure. © 2007 DSMZ GmbH - All rights reserved Acetogenium medium Carrine Blank DSM strains: DSMZ Medium 172 marine Cytophaga medium Cytophaga (marine) medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium172.pdf 172. CYTOPHAGA (marine) MEDIUM Yeast extract (Difco) 1.0 g Tryptone (Difco) 1.0 g NaCl 24.7 g KCl 0.7 g MgSO4 x 7 H2O 6.3 g MgCl2 x 6 H2O 4.6 g CaCl2 x 2 H2O 1.2 g NaHCO3 0.2 g Agar (Difco) 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2. Sodium bicarbonate and calcium chloride are autoclaved separately, each in a small volume of distilled water. © 2007 DSMZ GmbH - All rights reserved Cytophaga marine medium Cytophaga medium An organic-rich, solid culture medium comprised of yeast extract, tryptone, sodium chloride, potassium chloride, magnesium sulfate, magnesium chloride, calcium chloride, sodium bicarbonate, and agar. DSM strains: Carrine Blank mineral solution 2 for DSMZ Medium 165 Carrine Blank An inorganic salts solution comprised of potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, and calcium chloride. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium165.pdf 165. ACETIVIBRIO CELLULOLYTICUS MEDIUM Mineral solution 1 (see below) 75.000 ml Mineral solution 2 (see below) 75.000 ml FeSO4 x 7 H2O 0.020 g Vitamin solution (see medium 141) 10.000 ml Trace element solution (see medium 141) 10.000 ml Cellobiose or cellulose 3.000 g (MN 300, Whatman CF II, Kleenex tissue paper, or HCl treated cotton) Resazurin 0.001 g NaHCO3 2.000 g Cysteine-HCl x H2O 0.250 g Na2S x 9 H2O 0.250 g Distilled water 830.000 ml Adjust pH to 7.6. Gas atmosphere: 80% N2 + 20% CO2. Final pH after autoclaving is 7.2. Cellobiose is filter-sterilized separately, flushed and stored under nitrogen gas until needed. Cysteine and sodium sulfide are each autoclaved separately in concentrated solutions under nitrogen gas. Mineral solution 1: K2HPO4 3.900 g Distilled water 1000.000 ml Mineral solution 2: KH2PO4 2.400 g (NH4)2SO4 6.000 g NaCl 0.590 g MgSO4 x 7 H2O 1.200 g CaCl2 x 2 H2O 0.720 g Distilled water 1000.000 ml © 2007 DSMZ GmbH - All rights reserved trace elements solution for DSMZ Medium 150a Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium150a.pdf 150a. THIOBACILLUS CALDUS MEDIUM Use medium 150 without yeast extract. Adjust pH to 2.5 with sulfuric acid. After autoclaving add 10 ml/l of filter-sterilized trace elements solution (see below) and 5 g/l of sulfur powder (sterilized by steaming for 3 hours on each 3 successive days). Trace element solution: FeCl3 x 6 H2O 11.0 mg CuSO4 x 5 H2O 0.5 mg H3BO3 2.0 mg MnSO4 x H2O 2.0 mg Na2MoO4 x 2 H2O 0.8 mg CoCl2 x 6 H2O 0.6 mg ZnSO4 x 7 H2O 0.9 mg Distilled water 10.0 ml © 2010 DSMZ GmbH - All rights reserved A trace elements solution comprised of ferric trichloride, copper sulfate, boric acid, manganese sulfate, sodium molybdate, cobalt chloride, and zinc sulfate. filter-sterilized catalase Carrine Blank A defined organic solution containing the enzyme catalase, sterilized by passing it through a 0.2 µm filter. DSMZ Medium 172a An organic-rich, solid culture medium comprised of ammonium chloride, potassium phosphate, magnesium sulfate, calcium chloride, sodium chloride, ferric trichloride, yeast extract, agar, glucose, sodium bicarbonate, and sodium sulfide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium172a.pdf 172a. CY S2 MEDIUM NH4Cl 1.00 g KH2PO4 1.00 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 0.06 g NaCl 30.00 g FeCl3 x 6 H2O 1.25 mg Yeast extract 1.00 g Agar 1.00 g Distilled water 1000.00 ml Adjust pH to 7.0 and autoclave. After autoclaving, add from separate sterile solutions per liter medium: Glucose (10% w/v) 10.0 ml NaHCO3 (5% w/v) 10.0 ml Na2S x 9 H2O (3% w/v) 3.3 ml Aseptically fill sterile 10-ml screwcap tubes almost completely with the medium. Make stab inoculation. © 2007 DSMZ GmbH - All rights reserved CY S2 medium Carrine Blank DSM strains: DSMZ Medium 173 An organic-rich, liquid culture medium comprised of glucose, trypticase (trypticase peptone), yeast extract, resazurin, sea water, tris buffer (or potassium phosphate), and cysteine hydrochloride. Prepared under an atmosphere of dinitrogen. DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium173.pdf 173. SPIROCHAETA LITORALIS MEDIUM Glucose 2.0 g Trypticase (BBL) 1.0 g Yeast extract (Difco) 1.0 g Resazurin 1.0 mg Distilled water 200.0 ml Sea water 750.0 ml 1 M Tris-HCl buffer, pH 7.5 50.0 ml Cysteine-HCl x H2O 0.5 g Adjust pH to 7.5 prior to autoclaving. Add 1% agar (Difco) for stabs. Prereduced medium is prepared under 100% nitrogen atmosphere. The Tris-HCl buffer can be replaced by potassium phosphate buffer (1 M, pH 7.4) which is autoclaved separately. After adding to the main part of the medium some precipitate develops. Final pH is about 7.2. © 2007 DSMZ GmbH - All rights reserved Spirochaeta litoralis medium DSMZ Medium 174 Spirochaeta stenotrepta medium Carrine Blank DSM strains: An organic-rich liquid culture medium comprised of glucose, yeast extract, peptone, resazurin, and thioglycolate. Prepared under an atmosphere of dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium174.pdf 174. SPIROCHAETA STENOSTREPTA MEDIUM Glucose 5.0 g Yeast extract (Difco) 2.0 g Peptone (Difco) 2.0 g Resazurin 1.0 mg Thioglycolate 0.5 g Distilled water 1000.0 ml Adjust pH to 7.6 with KOH before autoclaving. Add 1% agar for stabs. Prereduced medium is prepared under 100% nitrogen atmosphere. Strain is sensitive to phosphates! Pay close attention to clean glassware! © 2009 DSMZ GmbH - All rights reserved DSMZ Medium 182 DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium182.pdf 182. SULFOLOBUS MEDIUM Yeast extract (DIFCO) 1.00 g Casamino acids (DIFCO) 1.00 g KH2PO4 3.10 g (NH4)2SO4 2.50 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.25 g Trace element solution SL-10 (see medium 320) 10.00 ml Distilled water 1000.00 ml Adjust pH to 4.0 - 4.2 with 10 N H2SO4 at room temperature. © 2009 DSMZ GmbH - All rights reserved Sulfolobus medium Carrine Blank An organic, liquid culture medium comprised of yeast extract, casamino acids, potassium phosphate, ammonium sulfate, magnesium sulfate, calcium chloride, and trace elements. DSMZ Medium 181 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium181.pdf 181. SELENOMONAS RUMINANTIUM MEDIUM Glucose 1.0 g Trypticase (BBL) 5.0 g KH2PO4 1.0 g Na-acetate 4.0 g Yeast extract 2.0 g n-Valeric acid 0.1 ml Resazurin 1.0 mg Na2CO3 4.0 g Cysteine-HCl x H2O 0.5 g Distilled water 1000.0 ml Adjust pH to 7.0. Gas atmosphere: 100% CO2. © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of glucose, trypticase (trypticase peptone), potassium phosphate, sodium acetate, yeast extract, valeric acid, resazurin, sodium carbonate, and cysteine hydrochloride. Prepared under an atmosphere of carbon dioxide. DSM strains: Selenomonas ruminantium medium Carrine Blank DSMZ Medium 183 Trichococcus medium Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium183.pdf 183. TRICHOCOCCUS MEDIUM Tryptone (Difco) 10.0 g Glucose 3.0 g MgCl2 x 6 H2O 1.1 g Na2SO4 4.0 g KCl 0.7 g Distilled water 1000.0 ml Adjust pH to 7.3. Glucose is filter-sterilized. © 2007 DSMZ GmbH - All rights reserved An organic, liquid culture medium comprised of tryptone, glucose, magnesium chloride, sodium sulfate, and potassium chloride. DSMZ Medium 182a An organic-rich, liquid culture medium comprised of yeast extract, potassium phosphate, ammonium sulfate, magnesium sulfate, calcium chloride, and trace elements (SL-10). DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium182a.pdf 182a. SULFOLOBUS MT-4 MEDIUM Yeast extract (Difco) 2.00 g KH2PO4 3.10 g (NH4)2SO4 2.50 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.25 g Trace element solution (see medium 320) 10.00 ml Distilled water 1000.00 ml Adjust pH of the medium at room temperature to 3.5 with 10 N H2SO4 prior to autoclaving. © 2009 DSMZ GmbH - All rights reserved Sulfolobus MT-4 medium Carrine Blank DSMZ Medium 185 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium185.pdf 185. THERMOPROTEUS MEDIUM (NH4)2SO4 0.264 g FeSO4 x 7 H2O 0.556 g MgSO4 x 7 H2O 0.492 g CaSO4 x 2 H2O 0.344 g KH2PO4 0.014 g Resazurin 1.000 mg Trace elements solution (see below) 1.000 ml Yeast extract 0.200 g Soluble starch 5.000 g Sulfur, powdered 10.000 g Na2S x 9 H2O 0.500 g Distilled water 1000.000 ml Trace elements solution: NaF 840.0 mg MnCl2 x 4 H2O 180.0 mg Na2B4O7 x 10 H2O 450.0 mg ZnSO4 x 7 H2O 22.0 mg CuCl2 x 2 H2O 5.0 mg Na2MoO4 x 2 H2O 3.0 mg CoSO4 x 7 H2O 1.0 mg Distilled water 1000.0 ml Prepare medium (without starch, sodium sulfide, sulfur) anaerobically under N2 gas atmosphere. Adjust pH to 5.5 with H2SO4 before sterilization. Distribute the medium into tubes containing the appropriate amount of sulfur powder. Sterilize medium by heating for 3 h at 90 - 100˚C on three subsequent days. Before use, add to the medium starch and sodium sulfide. Medium pH is 5.5. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank An organic-rich, liquid culture medium comprised of ammonium sulfate, ferrous sulfate, magnesium sulfate, calcium sulfate, potassium phosphate, resazurin, trace elements, yeast extract, soluble starch, elemental sulfur, and sodium sulfide. Prepared under an atmosphere of dinitrogen. Thermoproteus medium trace elements solution for DSMZ Medium 185 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium185.pdf 185. THERMOPROTEUS MEDIUM (NH4)2SO4 0.264 g FeSO4 x 7 H2O 0.556 g MgSO4 x 7 H2O 0.492 g CaSO4 x 2 H2O 0.344 g KH2PO4 0.014 g Resazurin 1.000 mg Trace elements solution (see below) 1.000 ml Yeast extract 0.200 g Soluble starch 5.000 g Sulfur, powdered 10.000 g Na2S x 9 H2O 0.500 g Distilled water 1000.000 ml Trace elements solution: NaF 840.0 mg MnCl2 x 4 H2O 180.0 mg Na2B4O7 x 10 H2O 450.0 mg ZnSO4 x 7 H2O 22.0 mg CuCl2 x 2 H2O 5.0 mg Na2MoO4 x 2 H2O 3.0 mg CoSO4 x 7 H2O 1.0 mg Distilled water 1000.0 ml Prepare medium (without starch, sodium sulfide, sulfur) anaerobically under N2 gas atmosphere. Adjust pH to 5.5 with H2SO4 before sterilization. Distribute the medium into tubes containing the appropriate amount of sulfur powder. Sterilize medium by heating for 3 h at 90 - 100˚C on three subsequent days. Before use, add to the medium starch and sodium sulfide. Medium pH is 5.5. © 2007 DSMZ GmbH - All rights reserved Carrine Blank A trace elements solution comprised of sodium fluoride, manganese chloride, sodium tetraborate, zinc sulfate, copper chloride, sodium molybdate, and cobalt sulfate. DSMZ Medium 187 An organic, solid culture medium comprised of sucrose, malt extract, yeast extract, and agar. DSM strains: Carrine Blank medium for osmophilic fungi M 40 Y http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium187.pdf 187. MEDIUM FOR OSMOPHILIC FUNGI (M 40 Y) Sucrose 400.0 g Malt extract 20.0 g Yeast extract 5.0 g Agar 20.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 186 Carrine Blank DSM strains: An organic-rich, solid culture medium comprised of yeast extract, malt extract, peptone from soybeans (soy peptone), glucose, and agar. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium186.pdf 186. UNIVERSAL MEDIUM FOR YEASTS (YM) Yeast extract 3.0 g Malt extract 3.0 g Peptone from soybeans 5.0 g Glucose 10.0 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved YM universal medium for yeasts DSMZ Medium 188 Carrine Blank An organic-rich, solid culture medium comprised of sodium nitrate, magnesium sulfate, potassium chloride, ferric sulfate, potassium phosphate, agar, yeast extract, and filter paper. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium188.pdf 188. CHAETOMIUM MEDIUM NaNO3 2.00 g MgSO4 x 7 H2O 0.50 g KCl 0.50 g Fe2(SO4)3 x H2O 0.01 g KH2PO4 0.14 g K2HPO4 1.20 g Agar 15.00 g Yeast extract 0.02 g Distilled water 1000.00 ml Adjust pH to 7.2. Place a strip of sterile filter paper on cooled agar and inoculate on filter strip. © 2007 DSMZ GmbH - All rights reserved DSM strains: Chaetomium medium DSMZ Medium 190 An organic-rich, solid culture medium comprised of yeast extract, soluble starch, potassium phosphate, magnesium sulfate, and agar. A solution of K2HPO4 will have a pH around 8.0. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium190.pdf 190. YpSs MEDIUM Yeast extract 4.0 g Soluble starch 15.0 g K2HPO4 1.0 g MgSO4 x 7 H2O 0.5 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved DSM strains: YpSs medium Carrine Blank DSMZ Medium 189 Oat flake medium An organic-rich, solid culture medium comprised of boiled oat flakes (boiled rolled oats) and agar. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium189.pdf 189. OAT FLAKE MEDIUM Oat flakes 30.0 g Agar 15.0 g Distilled water 1000.0 ml Boil oat flakes in water for 10 min; fill up to 1 l and add agar. Autoclave 20 min at 121˚C. Shake before pouring. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 191 An organic-rich, solid culture medium comprised of corn meal extract and agar. Carrine Blank corn meal medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium191.pdf 191. CORN MEAL AGAR Blend 50.0 g corn meal in 800 ml of distilled water. Leave overnight in the refrigerator. Heat at 60˚C for one hour. Filter and make up volume to 1 l. Add 10.0 g agar and heat to dissolve, then autoclave. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 192 A organic-rich, solid culture medium comprised of yeast extract, sodium acetate, ammonium sulfate, magnesium sulfate, potassium phosphate, thiamine hydrochloride, hemin, and agar. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium192.pdf 192. PILOBOLUS MEDIUM Yeast extract 2.00 g Na-acetate 10.00 g (NH4)2SO4 0.66 g MgSO4 x 7 H2O 0.50 g K2HPO4 1.00 g Thiamine-HCl x 2 H2O 10.00 mg Haemin (dissolved in 10.00 mg 37.5 ml 0.1 N NaOH) Agar 15.00 g Distilled water 1000.00 ml Adjust pH to 8.0. © 2007 DSMZ GmbH - All rights reserved Pilobus medium Carrine Blank DSM strains: DSMZ Medium 193 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved Carrine Blank A minerals-salts, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potasium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Desulfobacter sp. medium I Desulfobacter sp. medium DSM strains: DSMZ Medium 193.1 Carrine Blank Similar to DSMZ Medium 193, except sodium L-lactate and yeast extract are added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 193.1 -< for DSM 1744, DSM 10631, and DSM 16697 DSM 1744 is Desulfovibrio vulgaris DSM 10631 is Desulfovibrio aespoeensis Aspo-2 DSM 16697 is Desulfomicrobium thermophilum DSM 16697 DSMZ Medium 193.2 Carrine Blank Similar to DSMZ Medium 193, except sodium acetate is added. DSMZ Medium 193.2 -< for DSM 2034 DSM 2034 is Desulfobacter postgatei 2ac9 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 193.4 Similar to DSMZ Medium 193, except phenol and seven vitamins solution are added. Carrine Blank DSM 4660 is Desulfatiglans anilini DSM 4660 DSMZ Medium 193.4 -< for DSM 4660 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 193.5 Similar to DSMZ Medium 193, except potassium nitrate, acetate, and sodium pyruvate are added and sodium sulfide is omitted. DSMZ Medium 193.5 -< for DSM 6637 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved DSM 6637 is Paracoccus solventivorans DSMZ Medium 193.6 Carrine Blank DSMZ Medium 193.6 -< for DSM 8775 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 193, except sodium pyruvate is added. DSM 8775 is Desulfotomaculum sp. DSM 8775 DSMZ Medium 193.7 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSM 10711 is Desulfovibrio inopinatus DSM 10711 DSMZ Medium 193.7 -< for DSM 10711 Similar to DSMZ Medium 193, except sodium pyruvate, yeast extract, and seven vitamins solution are added. DSMZ Medium 193.8 DSMZ Medium 193.8 -< for DSM 14956 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 193, except methanol is added. DSM 14956 is Desulfotomaculum solfataricum DSMZ Medium 193.9 Similar to DSMZ Medium 193, except glucose and yeast extract are added. DSM 15102 is Garciella nitratireducens DSM 15102 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 193.9 -< for DSM 15102 Carrine Blank DSMZ Medium 193.10 Carrine Blank DSMZ Medium 193.10 -< for DSM 18843 Similar to DSMZ Medium 193, except sodium stearate and seven vitamins solution are added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved DSM 18843 is Desulfatiferula olefinivorans DSMZ Medium 193.11 DSM 25524 is Desulfatiferula berrensis Similar to DSMZ Medium 193, except sodium octanoate and seven vitamins solution are added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 193.11 -< for DSM 25524 DSMZ Medium 193.3 DSM 2059 is Desulfococcus multivorans DSM 2059 DSM 27197 is Desulfatiglans parachlorophenolica DSMZ Medium 193.3 -< for DSM 2059 and DSM 27197 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 193, except sodium benzoate is added. DSMZ Medium 193b http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193b.pdf 193b. DESULFOFABA MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g KCl 0.50 g MgCl2 x 6 H2O 0.60 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 860.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 503) 1.00 ml Solution F: CaCl2 x 2 H2O 1.50 g MgCl2 x 6 H2O 11.50 g Distilled water 20.00 ml Solution G: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D, F and G are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to G are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. For DSM 2075 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. © 2014 DSMZ GmbH - All rights reserved Carrine Blank A minerals-salts, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, potassium chloride, magnesium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium propionate, vitamins, calcium chloride, magnesium chloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: Desulfofaba medium DSMZ Medium 193b.1 Similar to DSMZ Medium 193b, except sodium propionate is omitted and sodium butyrate, sodium caproate, and sodium octanoate are added. Carrine Blank DSM 2075 is Desulfarculus baarsii DSM 2075 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193b.pdf 193b. DESULFOFABA MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g KCl 0.50 g MgCl2 x 6 H2O 0.60 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 860.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 503) 1.00 ml Solution F: CaCl2 x 2 H2O 1.50 g MgCl2 x 6 H2O 11.50 g Distilled water 20.00 ml Solution G: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D, F and G are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to G are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. For DSM 2075 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 193b.1 -< for DSM 2075 DSMZ Medium 193a An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, poly-(R)-3-hydroxybutyric acid, resazurin, trace elements, sodium bicarbonate, sodium pyruvate, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide. DSM strains: PHB/pyruvate medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193a.pdf 193a. PHB/PYRUVATE MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Poly[(R)-3-hydroxybutyric acid] (SIGMA) 1.00 g Resazurin 1.00 mg Distilled water 860.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-pyruvate 5.00 g Distilled water 20.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% H2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% H2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 6.8 - 7.0. After inoculation, pressurize vessels with 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 194.15 Similar to DSMZ Medium 194, except sodium propionate is omitted and sodium benzoate added. Carrine Blank DSM 28570 is Desulfoprunum benzoelyticum DSMZ Medium 194.15 -< for DSM 28570 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 194.14 DSM 15970 is Parasporobacterium paucivorans DSM 15970 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 194.14 -< for DSM 15970 Similar to DSMZ Medium 194, except sodium propionate is omitted and syringic acid and sodium hydroxide are added. DSMZ Medium 194.13 DSMZ Medium 194.13 -< for DSM 14880 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 14880 is Desulfotomaculum nigrificans CO-1-SRB Similar to DSMZ Medium 194, except sodium propionate is omitted and yeast extract and sodium pyruvate are added. DSMZ Medium 194.12 Similar to DSMZ Medium 194, except sodium propionate is omitted and sodium butyrate is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved DSM 13527 is Desulforegula conservatrix Mb1Pa DSM 21556 is Desulfovibrio butyratiphilus DSMZ Medium 194.12 -< for DSM 13527 and DSM 21556 Carrine Blank DSMZ Medium 194.11 DSMZ Medium 194.11 -< for DSM 12016 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 12016 is Desulfovirga adipica Similar to DSMZ Medium 194, except sodium propionate is omitted and propanol and yeast extract are added. DSMZ Medium 194.10 DSM 10291 is Desulfovibrio sp. DSM 24454 is Desulfovibrio cf. magneticus IFRC170 Similar to DSMZ Medium 194, except sodium propionate is omitted and sodium lactate and seven vitamins solution are added. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 194.10 -< for DSM 10291 and DSM 24454 DSMZ Medium 194.9 Carrine Blank DSM 7474 is Desulfotomaculum sp. DSM 7475 is Desulfotomaculum sp. DSM 7476 is Desulfotomaculum sp. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 194.9 -< for DSM 7474, DSM 7475, and DSM 7476 Similar to DSMZ Medium 194, except sodium propionate is omitted and 3,4,5-trimethoxybenzoic acid and seven vitamins solution are added. DSMZ Medium 194.8 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved DSM 6283 is Anaeroarcus burkinensis DSM 6283 DSMZ Medium 194.8 -< for DSM 6283 Similar to DSMZ Medium 194, except sodium propionate is omitted and sodium lactate and yeast extract are added. The pH is reduced slightly to 6.8-7.0. DSMZ Medium 194.7 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved DSM 5651 is Desulfococcus biacutus DSMZ Medium 194.7 -< for DSM 5651 Similar to DSMZ Medium 194, except sodium propionate is omitted and sodium 3-hydroxybutyrate is added. DSMZ Medium 194.6 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 5502 is Oxalobacter vibrioformis DSM 5503 is Oxalophagus oxalicus Similar to DSMZ Medium 194, except sodium propionate is omitted and ammonium oxalate and yeast extract are added. The pH is reduced slightly to 6.8-7.0. DSMZ Medium 194.6 -< for DSM 5502 and DSM 5503 DSMZ Medium 194.5 DSM 5433 is Desulfovibrio alcoholivorans DSM 5433 DSMZ Medium 194.5 -< for DSM 5433 Similar to DSMZ Medium 194, except sodium propionate is omitted and 1,2-propanediol (propane-1,2-diol) is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 194.4 DSM 5193 is Acetobacterium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 194, except sodium propionate is omitted and yeast extract and sodium methoxyacetate are added. DSMZ Medium 194.4 -< for DSM 5193 Carrine Blank DSMZ Medium 194.3 DSM 5092 is Anaerovorax odorimutans DSM 5092 Similar to DSMZ Medium 194, except sodium propionate is omitted and putrescine is added. Carrine Blank DSMZ Medium 194.3 -< for DSM 5092 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 194.2 DSMZ Medium 194.2 -< for DSM 3852 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved DSM 3852 is Desulfovibrio carbinolicus Carrine Blank Similar to DSMZ Medium 194, except sodium propionate is omitted, and ethanol, yeast extract, and casamino acids are added. DSMZ Medium 194.1 Carrine Blank DSMZ Medium 194.1 -< for DSM 2055 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved DSM 2055 is Desulfobotulus sapovorans DSM 2055 Similar to DSMZ Medium 194, except sodium propionate is omitted and sodium butyrate, sodium caproate, and sodium octanoate are added. The pH is increased to 7.7. DSMZ Medium 194 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved A minerals-salts, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium propionate, vitamins, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of dinitrogen and carbon dioxide. Desulfobulbus sp. medium (freshwater) DSM strains: Carrine Blank Desulfobulbus sp. freshwater medium Desulfobulbus sp. medium DSMZ Medium 195 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195.pdf 195. DESULFOBACTER SP. MEDIUM (ACETATE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. © 2014 DSMZ GmbH - All rights reserved Desulfobacter sp. acetate medium A minerals-salts, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium acetate, vitamins, sodium dithionite, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: Desulfobacter sp. medium Desulfobacter sp. medium (acetate) DSMZ Medium 195a Desulfobacter sp. fumarate medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195a.pdf 195a. DESULFOBACTER SP. MEDIUM (FUMARATE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na2-fumarate 2.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. © 2014 DSMZ GmbH - All rights reserved Carrine Blank Desulfobacter sp. medium (fumarate) Desulfobacter sp. medium An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate solution, resazurin, trace elements, sodium bicarbonate, sodium fumarate, vitamins, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 195b http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195b.pdf 195b. DESULFOBULBUS SP. MEDIUM (MARINE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium propionate, vitamins, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of carbon dioxide and dinitrogen. Desulfobulbus sp. marine medium Desulfobulbus sp. medium (marine) Desulfobulbus sp. medium DSMZ Medium 195c.5 Similar to DSMZ Medium 195c, except casamino acids and trypticase pepetone are added. DSM 16109 is Desulfovibrio alaskensis DSM 16109 DSM 17464 is Desulfovibrio alaskensis G20 DSMZ Medium 195c.5 -< for DSM 16109 and DSM 17464 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195c.pdf 195c. DESULFOBACTER SP. MEDIUM (LACTATE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. For DSM 9120, DSM 10141, DSM 17456 and DSM 19275 supplement medium with 1 g/l yeast extract added to the autoclaved medium from a sterile anoxic stock solution. For DSM 11974 supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 14278 supplement medium with 0.5 g/l Na-acetate added to the autoclaved medium from a sterile anoxic stock solution. For DSM 14982 supplement medium with 5.0 g/l Na2S2O3 x 5 H2O added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 16109 and DSM 17464 supplement medium with 2.0 g/l Casamino acids (BACTO) and 2.0 g/l Trypticase peptone (BD BBL) added to the autoclaved medium from sterile anoxic stock solutions. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 195c.4 DSMZ Medium 195c.4 -< for DSM 14982 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195c.pdf 195c. DESULFOBACTER SP. MEDIUM (LACTATE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. For DSM 9120, DSM 10141, DSM 17456 and DSM 19275 supplement medium with 1 g/l yeast extract added to the autoclaved medium from a sterile anoxic stock solution. For DSM 11974 supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 14278 supplement medium with 0.5 g/l Na-acetate added to the autoclaved medium from a sterile anoxic stock solution. For DSM 14982 supplement medium with 5.0 g/l Na2S2O3 x 5 H2O added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 16109 and DSM 17464 supplement medium with 2.0 g/l Casamino acids (BACTO) and 2.0 g/l Trypticase peptone (BD BBL) added to the autoclaved medium from sterile anoxic stock solutions. © 2014 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 195c, except sodium thiosulfate is added. Carrine Blank DSM 14982 is Desulfovibrio capillatus DSMZ Medium 195c.3 DSM 14278 is not in www.dsmz.de, Tax Browser, or StrainInfo. DSMZ Medium 195c.3 -< for DSM 14278 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195c.pdf 195c. DESULFOBACTER SP. MEDIUM (LACTATE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. For DSM 9120, DSM 10141, DSM 17456 and DSM 19275 supplement medium with 1 g/l yeast extract added to the autoclaved medium from a sterile anoxic stock solution. For DSM 11974 supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 14278 supplement medium with 0.5 g/l Na-acetate added to the autoclaved medium from a sterile anoxic stock solution. For DSM 14982 supplement medium with 5.0 g/l Na2S2O3 x 5 H2O added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 16109 and DSM 17464 supplement medium with 2.0 g/l Casamino acids (BACTO) and 2.0 g/l Trypticase peptone (BD BBL) added to the autoclaved medium from sterile anoxic stock solutions. © 2014 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 195c, except sodium acetate is added. DSMZ Medium 195c.2 Similar to DSMZ Medium 195c, except seven vitamins solution is added. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195c.pdf 195c. DESULFOBACTER SP. MEDIUM (LACTATE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. For DSM 9120, DSM 10141, DSM 17456 and DSM 19275 supplement medium with 1 g/l yeast extract added to the autoclaved medium from a sterile anoxic stock solution. For DSM 11974 supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 14278 supplement medium with 0.5 g/l Na-acetate added to the autoclaved medium from a sterile anoxic stock solution. For DSM 14982 supplement medium with 5.0 g/l Na2S2O3 x 5 H2O added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 16109 and DSM 17464 supplement medium with 2.0 g/l Casamino acids (BACTO) and 2.0 g/l Trypticase peptone (BD BBL) added to the autoclaved medium from sterile anoxic stock solutions. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 195c.2 -< for DSM 11974 DSM 11974 is Desulfovibrio zosterae DSM 11974 DSMZ Medium 195c.1 DSM 9120 is Desulfobacterium zeppelinii DSM 10141 is Desulfovibrio acrylicus DSM 10141 DSM 17456 is Desulfovibrio marinisediminis DSM 17456 DSM 19275 is Desulfovibrio tunisiensis DSM 19275 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195c.pdf 195c. DESULFOBACTER SP. MEDIUM (LACTATE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. For DSM 9120, DSM 10141, DSM 17456 and DSM 19275 supplement medium with 1 g/l yeast extract added to the autoclaved medium from a sterile anoxic stock solution. For DSM 11974 supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 14278 supplement medium with 0.5 g/l Na-acetate added to the autoclaved medium from a sterile anoxic stock solution. For DSM 14982 supplement medium with 5.0 g/l Na2S2O3 x 5 H2O added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 16109 and DSM 17464 supplement medium with 2.0 g/l Casamino acids (BACTO) and 2.0 g/l Trypticase peptone (BD BBL) added to the autoclaved medium from sterile anoxic stock solutions. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 195c.1 -< for DSM 9120, DSM 10141, DSM 17456 and DSM 19275 Similar to DSMZ Medium 195c, except yeast extract is added. DSMZ Medium 195c Desulfobacter sp. lactate medium An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium lactate, vitamins, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of dinitrogen and carbon dioxide. Desulfobacter sp. medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195c.pdf 195c. DESULFOBACTER SP. MEDIUM (LACTATE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. For DSM 9120, DSM 10141, DSM 17456 and DSM 19275 supplement medium with 1 g/l yeast extract added to the autoclaved medium from a sterile anoxic stock solution. For DSM 11974 supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 14278 supplement medium with 0.5 g/l Na-acetate added to the autoclaved medium from a sterile anoxic stock solution. For DSM 14982 supplement medium with 5.0 g/l Na2S2O3 x 5 H2O added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 16109 and DSM 17464 supplement medium with 2.0 g/l Casamino acids (BACTO) and 2.0 g/l Trypticase peptone (BD BBL) added to the autoclaved medium from sterile anoxic stock solutions. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: Desulfobacter sp. medium (lactate) DSMZ Medium 202 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium202.pdf 202. DESULFONEMA MAGNUM MEDIUM Solution A: NaCl 25.00 g MgCl2 x 6 H2O 5.60 g MgSO4 x 7 H2O 6.80 g CaCl2 x 2 H2O 1.40 g KCl 0.72 g KH2PO4 0.14 g NH4Cl 0.25 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 1000.00 ml Solution B: Na-benzoate 0.60 g Distilled water 10.00 ml Solution C: Vitamin solution (see medium 141) 10.00 ml Solution D: KAl(SO4)2 x 12 H2O 0.48 g Distilled water 10.00 ml Solution E: Na2CO3 1.00 g Distilled water 20.00 ml Solution F: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Solution A is boiled for a 1 min, cooled to room temperature, while gassing with 80% N2 and 20% CO2 gas mixture to reach a pH of around 6, then distributed anoxically in cultivation vessels and autoclaved under the same gas atmosphere. Solutions B, D, and F are autoclaved separately under 100% N2 gas, solution E is flushed with 80% N2 and 20% CO2 gas mixture to remove dissolved oxygen and autoclaved, solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions B to F are added to the autoclaved solution A in the sequence as indicated. Final pH of the medium should be 7.0. After completion the medium should equilibrate overnight and a white precipitate should be apparent. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) just before inoculation may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. © 2010 DSMZ GmbH - All rights reserved A minerals-salts, liquid culture medium comprised of sodium chloride, magnesium chloride, magnesium sulfate, calcium chloride, potassium chloride, potassium phosphate, ammonium chloride, trace elements, selenite-tungstate, resazurin, sodium benzoate, vitamins, potassium aluminum sulfate, sodium carbonate, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: Carrine Blank Desulfonema magnum medium DSMZ Medium 198.2 Similar to DSMZ Medium 198, except sodium benzoate is omitted and sodium acetate is added. Carrine Blank DSMZ Medium 198.2 -< for DSM 9990 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium198.pdf 198. DESULFOSARCINA MEDIUM (BRACKISH WATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 13.50 g MgCl2 x 6 H2O 2.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2650 Na-benzoate is replaced by 1.50 g/l Na-pyruvate and 1.00 g/l Na2-malate added after autoclaving from sterile anoxic stock solutions prepared under N2. For DSM 9990 Na-benzoate is replaced by 6.20 g/l Na-acetate. © 2015 DSMZ GmbH - All rights reserved DSM 9990 is Thermodesulforhabdus norvegica DSMZ Medium 198.1 DSM 2650 is Desulfobacterium niacini http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium198.pdf 198. DESULFOSARCINA MEDIUM (BRACKISH WATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 13.50 g MgCl2 x 6 H2O 2.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2650 Na-benzoate is replaced by 1.50 g/l Na-pyruvate and 1.00 g/l Na2-malate added after autoclaving from sterile anoxic stock solutions prepared under N2. For DSM 9990 Na-benzoate is replaced by 6.20 g/l Na-acetate. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 198, except sodium benzoate is omitted and sodium pyruvate and sodium malate are added. Carrine Blank DSMZ Medium 198.1 -< for DSM 2650 DSMZ Medium 198 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium198.pdf 198. DESULFOSARCINA MEDIUM (BRACKISH WATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 13.50 g MgCl2 x 6 H2O 2.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2650 Na-benzoate is replaced by 1.50 g/l Na-pyruvate and 1.00 g/l Na2-malate added after autoclaving from sterile anoxic stock solutions prepared under N2. For DSM 9990 Na-benzoate is replaced by 6.20 g/l Na-acetate. © 2015 DSMZ GmbH - All rights reserved DSM strains: A minerals-salts, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium benzoate, vitamins, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of dinitrogen and carbon dioxide. Desulfosarcina medium (brackish water) Desulfosarcina medium Desulfosarcina brackish medium Carrine Blank DSMZ Medium 203.1 DSM 3496 is Methanothermus sociabilis Similar to DSMZ Medium 203, except the yeast extract, trypticase peptone, and vitamins are omitted. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium203.pdf 203. METHANOTHERMUS FERVIDUS MEDIUM Mineral solution 1 (see below) 37.5 ml Mineral solution 2 (see below) 37.5 ml Ni(NH4)2(SO4)2 2.0 mg or NiCl2 x 6 H2O 1.0 mg FeSO4 x 7 H2O 2.0 mg Yeast extract 2.0 g Trypticase (BBL) 2.0 g Trace element solution (see medium 141) 10.0 ml Na2SO4 3.4 g Resazurin 1.0 mg NaHCO3 2.0 g Vitamin solution (see medium 141) 10.0 ml Na2S x 9 H2O 0.5 g L-Cysteine-HCl x H2O 0.5 g Distilled water 920.0 ml Mineral solution 1: K2HPO4 6.0 g Distilled water 1000.0 ml Mineral solution 2: KH2PO4 6.0 g (NH4)2SO4 6.0 g NaCl 12.0 g MgSO4 x 7 H2O 2.4 g CaCl2 x 2 H2O 1.6 g Distilled water 1000.0 ml Dissolve ingredients except bicarbonate, sulfide and vitamins, sparge medium with 80% N2 and 20% CO2 gas mixture for at least 30 – 45 min to make it anoxic, then add bicarbonate and adjust pH to 6.5. Add sodium sulfide and distribute medium under 80% N2 and 20% CO2 gas atmosphere into soda lime glass serum bottles (20 ml of medium per 100 ml bottle). Pressurize to 2 bar 80% H2 and 20% CO2 overpressure, then autoclave! Prior to inoculation add vitamins from a sterile anoxic stock solution sterilized by filtration and adjust pH of completed medium to 6.5. For DSM 3496 omit yeast extract, trypticase and vitamins. Alternative procedure: Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide, then adjust pH to 6.0 with sulfuric acid. Bring medium to the boil, then cool to room temperature while gassing with 80% H2 and 20% CO2 gas mixture. Dispense under same gas atmosphere in glass serum bottles, seal, and autoclave without gas overpressure. Thereafter, add by injection vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and hydrogencarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to 6.5. After inoculation pressurize bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. © 2012 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 203.1 -< for DSM 3496 DSMZ Medium 203 Carrine Blank Methanothermus fervidus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium203.pdf 203. METHANOTHERMUS FERVIDUS MEDIUM Mineral solution 1 (see below) 37.5 ml Mineral solution 2 (see below) 37.5 ml Ni(NH4)2(SO4)2 2.0 mg or NiCl2 x 6 H2O 1.0 mg FeSO4 x 7 H2O 2.0 mg Yeast extract 2.0 g Trypticase (BBL) 2.0 g Trace element solution (see medium 141) 10.0 ml Na2SO4 3.4 g Resazurin 1.0 mg NaHCO3 2.0 g Vitamin solution (see medium 141) 10.0 ml Na2S x 9 H2O 0.5 g L-Cysteine-HCl x H2O 0.5 g Distilled water 920.0 ml Mineral solution 1: K2HPO4 6.0 g Distilled water 1000.0 ml Mineral solution 2: KH2PO4 6.0 g (NH4)2SO4 6.0 g NaCl 12.0 g MgSO4 x 7 H2O 2.4 g CaCl2 x 2 H2O 1.6 g Distilled water 1000.0 ml Dissolve ingredients except bicarbonate, sulfide and vitamins, sparge medium with 80% N2 and 20% CO2 gas mixture for at least 30 – 45 min to make it anoxic, then add bicarbonate and adjust pH to 6.5. Add sodium sulfide and distribute medium under 80% N2 and 20% CO2 gas atmosphere into soda lime glass serum bottles (20 ml of medium per 100 ml bottle). Pressurize to 2 bar 80% H2 and 20% CO2 overpressure, then autoclave! Prior to inoculation add vitamins from a sterile anoxic stock solution sterilized by filtration and adjust pH of completed medium to 6.5. For DSM 3496 omit yeast extract, trypticase and vitamins. Alternative procedure: Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide, then adjust pH to 6.0 with sulfuric acid. Bring medium to the boil, then cool to room temperature while gassing with 80% H2 and 20% CO2 gas mixture. Dispense under same gas atmosphere in glass serum bottles, seal, and autoclave without gas overpressure. Thereafter, add by injection vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and hydrogencarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to 6.5. After inoculation pressurize bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. © 2012 DSMZ GmbH - All rights reserved A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, ammonium nickel sulfate (or ammonium chloride), ferrous sulfate, yeast extract, trypticase (trypticase peptone), trace elements, sodium sulfate, resazurin, sodium bicarbonte, vitamins, sodium sulfide, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen, carbon dioxide, and dihydrogen. DSM strains: DSMZ Medium 204 An organic-rich, solid culture medium comprised of sodium nitrate, potassium phosphate, potassium chloride, magnesium sulfate, ferrous sulfate, zinc sulfate, agar, and glucose. Aspergillus nidulans minimal medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium204.pdf 204. ASPERGILLUS NIDULANS MINIMAL MEDIUM Solution A: NaNO3 6.00 g KH2PO4 1.52 g KCl 1.52 g Distilled water 500.00 ml Adjust pH to 6.5 with 2 N NaOH. Solution B: MgSO4 x 7 H2O 0.52 g FeSO4 x 7 H2O trace - ZnSO4 x 7 H2O trace - Agar 15.00 g Distilled water 250.00 ml Solution C: Glucose 10.00 g Distilled water 200.00 ml Autoclave separately for 15 min. Mix A, B and C before pouring plates. © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 205 Natronobacterium medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium205.pdf 205. NATRONOBACTERIUM MEDIUM Casamino acids 15.0 g Na3-citrate x 2 H2O 3.0 g Glutamic acid 2.5 g MgSO4 x 7 H2O 2.5 g KCl 2.0 g NaCl 250.0 g Agar 20.0 g Add distilled water to give a final volume of 1000.0 ml. Dissolve agar by heating before adding sodium chloride. Adjust pH to 7.0 before autoclaving. Adjust pH to 8.5 with sterile 5% Na2CO3 after heat sterilization of the medium. © 2007 DSMZ GmbH - All rights reserved Carrine Blank An organic-rich, solid culture medium comprised of casamino acids, sodium citrate, glutamic acid, magnesium sulfate, potassium chloride, sodium chloride, and agar. DSMZ Medium 206 Carrine Blank DSM strains: Thermodesulfobacterium medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium206.pdf 206. THERMODESULFOBACTERIUM MEDIUM Na2SO4 3.0 g NH4Cl 1.0 g MgCl2 x 6 H2O 0.2 g KH2PO4 0.3 g Na2HPO4 x 12 H2O 2.0 g Trace element solution (see medium 144) 10.0 ml FeSO4 x 7 H2O 1.5 mg Vitamin solution (see medium 141) 5.0 ml Resazurin 1.0 mg Yeast extract 1.0 g Na-L-lactate 4.0 g Na2S x 9 H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except yeast extract, lactate and sulfide) and sparge medium with 100% N2 gas to make it anoxic (c. 30 – 45 min). After sterilization add yeast extract, lactate, and sulfide from stock solutions prepared under N2 gas and autoclaved separately. Before use, neutralize the sodium sulfide solution by dropwise addition of 1 N HCl. Adjust pH of final medium to 6.8 - 7.0. © 2014 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of sodium sulfate, ammonium chloride, magnesium chloride, potassium phosphate, sodium phosphate, trace elements, ferrous sulfate, vitamins, resazurin, yeast extract, sodium lactate, and sodium sulfide. Prepared under an atmosphere of dinitrogen. DSMZ Medium 207 DSM strains: Carrine Blank An organic-rich, liquid culture medium comprised of potassium phosphate, sodium phosphate, ammonium chloride, magnesium chloride, trace elements, ferrous sulfate, vitamins, resazurin, sodium bicarbonate, yeast extract, sodium lactate, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Propionispira medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium207.pdf 207. PROPIONISPIRA MEDIUM KH2PO4 0.3 g Na2HPO4 x 12 H2O 2.1 g NH4Cl 1.0 g MgCl2 x 6 H2O 0.2 g Trace element solution (see medium 144) 10.0 ml FeSO4 x 7 H2O 1.5 mg Vitamin solution (see medium 141) 5.0 ml Resazurin 1.0 mg NaHCO3 4.0 g Yeast extract 2.0 g Na-lactate 5.0 g Na2S x 9 H2O 0.5 g Distilled water 1000.0 ml Adjust pH to 7.2. Gas atmosphere: 80% N2 + 20% CO2. Prepare a 5% (w/v) solution of sodium sulfide anoxically under nitrogen and autoclave separately. Before use, neutralize by dropwise addition of 1 N HCl. © 2013 DSMZ GmbH - All rights reserved DSMZ Medium 209 Carrine Blank Similar to DSMZ Medium 78, except arginine is added. Eubacterium lentum medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium209.pdf 209. EUBACTERIUM LENTUM MEDIUM To medium 78 add 0.5% (w/v) arginine. © 2007 DSMZ GmbH - All rights reserved boiled rolled oats Wikipedia:Oat Flaking This process uses two large smooth or corrugated rolls spinning at the same speed in opposite directions at a controlled distance. Oat flakes, also known as rolled oats, have many different sizes, thicknesses and other characteristics depending on the size of oat groats passed between the rolls. Typically, the three sizes of steel cut oats are used to make instant, baby and quick rolled oats, whereas whole oat groats are used to make regular, medium and thick rolled oats. Oat flakes range in thickness from 0.36 mm to 1.00 mm. boiled oat flakes Rolled oats that have been boiled (cooked in water). Carrine Blank DSMZ Medium 320 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium320.pdf 320. CLOSTRIDIUM CELLULOVORANS MEDIUM K2HPO4 x 3 H2O 1.00 g NH4Cl 1.00 g KCl 0.50 g MgSO4 x 7 H2O 0.50 g Trypticase peptone 0.50 g Yeast extract 0.50 g Rumen fluid, clarified or sludge fluid (see medium 119) 20.00 ml Trace element solution SL-10 (see below) 1.00 ml Resazurin 1.00 mg L-Cysteine-HCl x H2O 0.15 g Na2CO3 1.00 g Cellobiose 5.00 g Na2S x 9 H2O 0.15 g Distilled water 1000.00 ml Dissolve ingredients (except cysteine, carbonate, cellobiose and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture and add cysteine. Dispense under same gas atmosphere in culture vessels and autoclave. Add sulfide from a sterile anoxic stock solution prepared under N2 and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2. Sterilize cellobiose separately by filtration under N2 gas. Adjust pH of the completed medium to 7.0. Some strains can be adapted to cellulose as substrate using 10.0 g/l cellulose MN 301 (MACHEREY-NAGEL). 'Trace element solution SL-10: HCl (25%; 7.7 M) 10.00 ml FeCl2 x 4 H2O 1.50 g ZnCl2 70.00 mg MnCl2 x 4 H2O 100.00 mg H3BO3 6.00 mg CoCl2 x 6 H2O 190.00 mg CuCl2 x 2 H2O 2.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 36.00 mg Distilled water 990.00 ml First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml. © 2012 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, potassium chloride, magnesium sulfate, trypticase peptone, yeast extract, clarified rumen fluid or sludge fluid, trace elements, resazurin, L-cysteine hydrochloride, sodium carbonate, cellobiose, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Clostridium cellulovorans medium Carrine Blank DSM strains: corn meal extract An undefined organic chemical mixture comprised of the seed of corn (Zea mays), which has been ground, hydrated in water, and then heated. Finally, the liquid is filtered. Carrine Blank DSMZ Medium 211 DSM strains: Thermoanaerobacterium thermosulfurogenes medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium211.pdf 211. THERMOANAEROBACTERIUM THERMOSULFUROGENES MEDIUM KH2PO4 0.3 g Na2HPO4 x 12 H2O 5.3 g NH4Cl 1.0 g MgCl2 x 6 H2O 0.2 g Trace elements solution (see medium 144) 10.0 ml FeSO4 x 7 H2O 1.5 mg Yeast extract 1.0 g Resazurin 0.5 mg D-Glucose 5.0 g Vitamin solution (see medium 141) 5.0 ml Na2S x 9 H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except glucose, vitamins and sulfide), adjust pH to 6.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add glucose and sulfide from anoxic stock solutions autoclaved under 100% N2 gas and vitamins from a filter-sterilized anoxic stock solution prepared under N2. Adjust pH of final medium to 6.0 – 6.5. For DSM 8683, DSM 8684 and DSM 16487 adjust pH of completed medium to 5.5. © 2014 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of potassium phosphate, sodium phosphate, ammonium chloride, magnesium chloride, trace elements, ferrous sulfate, yeast extract, resazurin, D-glucose, vitamin solution, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen. Carrine Blank DSMZ Medium 211.1 DSM 8683 is Thermoanaerobacterium sp. DSM 8684 is Thermoanaerobacterium sp. DSM 16487 is Thermoanaerobacterium aciditolerans Similar to DSMZ Medium 211, except the pH is reduced to 5.5. DSMZ Medium 211.1 -< for DSM 8683, DSM 8684 and DSM 16487 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium211.pdf 211. THERMOANAEROBACTERIUM THERMOSULFUROGENES MEDIUM KH2PO4 0.3 g Na2HPO4 x 12 H2O 5.3 g NH4Cl 1.0 g MgCl2 x 6 H2O 0.2 g Trace elements solution (see medium 144) 10.0 ml FeSO4 x 7 H2O 1.5 mg Yeast extract 1.0 g Resazurin 0.5 mg D-Glucose 5.0 g Vitamin solution (see medium 141) 5.0 ml Na2S x 9 H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except glucose, vitamins and sulfide), adjust pH to 6.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add glucose and sulfide from anoxic stock solutions autoclaved under 100% N2 gas and vitamins from a filter-sterilized anoxic stock solution prepared under N2. Adjust pH of final medium to 6.0 – 6.5. For DSM 8683, DSM 8684 and DSM 16487 adjust pH of completed medium to 5.5. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 237 Carrine Blank DSM strains: An organic-rich, solid culture medium comprised of nutrient broth, peptone, potassium phosphate, sodium chloride, glucose, and agar. ENB medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium237.pdf 237. ENB MEDIUM Nutrient Broth 8.0 g Peptone 5.0 g KH2PO4 1.5 g K2HPO4 3.5 g NaCl 5.0 g Glucose 1.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 6.8 - 7.0. Sterilize glucose separately. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 233 A minerals-salts, liquid culture medium comprised of potassium chloride, magnesium chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, resazurin, sodium bicarbonate, vitamins, methanol, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium233.pdf 233. METHANOLOBUS TINDARIUS MEDIUM KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Resazurin 1.00 mg NaHCO3 1.00 g Vitamin solution (see medium 141) 10.00 ml Methanol 5.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 6.5, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add methanol, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.5. © 2014 DSMZ GmbH - All rights reserved Methanolobus tindarius medium Carrine Blank DSMZ Medium 232b http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium232b.pdf 232b. WEISSELA KOREENSIS MEDIUM Use medium 232 + 4 ml Resazurin solution (25 mg/100 ml). Cystein are added after the medium has been boiled and cooled und CO2. Distribute under N2 and autoclave. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank Similar to DSMZ Medium 232, except resazurine is added. Prepared under an atmosphere of carbon dioxide and dinitrogen. Weissela koreensis medium DSMZ Medium 231 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium231.pdf 231. PEDIOCOCCUS DAMNOSUS MEDIUM Add 0.05% cysteine-hydrochloride to medium 11 and adjust to pH 5.2. Medium should be filled up into crew capped tubes (Wheaton). © 2007 DSMZ GmbH - All rights reserved Pediococcus damnosus medium DSM strains: Similar to DSMZ Medium 11, except cysteine hydrochloride is added and the pH is reduced to 5.2. DSMZ Medium 232 MRS medium with cysteine Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium232.pdf 232. MRS MEDIUM WITH CYSTEINE Casein peptone, tryptic digest 10.00 g Meat extract 10.00 g Yeast extract 5.00 g Glucose 20.00 g Tween 80 1.00 g K2HPO4 2.00 g Na-acetate 5.00 g (NH4)2 citrate 2.00 g MgSO4 x 7 H2O 0.20 g MnSO4 x H2O 0.05 g Distilled water 1000.00 ml Adjust pH to 6.2 - 6.5. Add 0.05% cysteine-hydrochloride. © 2012 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of casein peptone, tryptic digest (casitone), meat extract, yeast extract, glucose, Tween 80 (polysorbate 80), potassium phosphate, sodium acetate, ammonium citrate, magnesium sulfate, manganese sulfate, and cysteine hydrochloride. DSM strains: DSMZ Medium 227 Pediococcus halophilus medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium227.pdf 227. PEDIOCOCCUS HALOPHILUS MEDIUM To medium 11 add 6.5% NaCl. © 2007 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 11, except sodium chloride is added. DSM strains: DSMZ Medium 225 DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium225.pdf 225. SOUR DOUGH MEDIUM Maltose 20.0 g Yeast extract 3.0 g Fresh yeast extract 15.0 ml Tween 80 0.3 g Casein peptone, tryptic digest 6.0 g Distilled water up to 1000.0 ml Adjust pH to 5.6 with 20% lactic acid or HCl. Fresh yeast extract is prepared by autoclaving a 20% suspension of commercial baker's yeast in distilled water for 30 minutes at 121˚C, allowing the suspension to settle overnight at 2 to 8˚C, decanting and further clarifying the supernatant by centrifugation. The extract prepared in this manner contains 1.5% solids and if not to be used within a few days, should be frozen or freeze-dried immediately. © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of maltose, yeast extract, autoclaved Baker's yeast, tween 80 (polysorbate 80), casein peptone, tryptic digest (casitone), lactic acid or hydrochloric acid. Carrine Blank sour dough medium DSMZ Medium 226 An organic-rich, liquid culture medium comprised of casamino acids, yeast extract, glucose, ammonium citrate, potassium phosphate, magnesium sulfate, ferric chloride, and acetylacetone. DSM strains: 226. AUREOBACTERIUM TERREGENS MEDIUM Casamino acids 2.0 g Yeast extract 1.0 g Glucose 1.0 g (NH4) citrate 1.0 g K2HPO4 2.0 g MgSO4 x 7 H2O 0.5 g FeCl3 x 6 H2O 10.0 mg Distilled water 1000.0 ml Adjust pH to 7.0. After sterilization, aseptically add 1 ml/l of a 10% solution of acetylacetone in ethanol. © 2007 DSMZ GmbH - All rights reserved Aureobacterium terregens medium Carrine Blank DSMZ Medium 222 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium222.pdf 222. SP - MEDIUM Raffinose 1.00 g Sucrose 1.00 g Galactose 1.00 g Soluble starch 5.00 g Casitone 2.50 g MgSO4 x 7 H2O 0.50 g K2HPO4 0.25 g Agar (Difco) 15.00 g Distilled water 1000.00 ml Adjust pH to 7.4. © 2007 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, solid culture medium comprised of raffinose, sucrose, galactose, soluble starch, casitone, magnesium sulfate, potassium phosphate, and agar. Carrine Blank SP medium DSMZ Medium 221 DSM strains: Azospirillum medium Carrine Blank An organic-rich, liquid culture medium comprised of yeast extract, potassium phosphate, ferrous sulfate, sodium molybdate, manganese sulfate, magnesium sulfate, sodium chloride, calcium chloride, ammonium sulfate, biotin, glucose and sodium malate (disodium malate). http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium221.pdf 221. AZOSPIRILLUM MEDIUM Yeast extract 0.05 g K2HPO4 0.25 g FeSO4 x 7 H2O 0.01 g Na2MoO4 x 2 H2O 1.00 mg MnSO4 x H2O 2.00 mg MgSO4 x 7 H2O 0.20 g NaCl 0.10 g CaCl2 x 2 H2O 0.02 g (NH4)2SO4 1.00 g Biotin 0.10 mg Distilled water 950.00 ml Adjust pH of the medium to 7.1. Add 1.5% agar if needed. Autoclave for 15 min at 121˚C. After sterilization add 25 ml each of filter-sterilized 20% glucose and 20% Na-malate. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 219 Carrine Blank Mycobacterium medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium219.pdf 219. MYCOBACTERIUM MEDIUM Yeast extract 2.0 g Proteose peptone no. 3 2.0 g Casein peptone, tryptic digested 2.0 g Na2HPO4 x 12 H2O 2.5 g KH2PO4 1.0 g Na-citrate 1.5 g MgSO4 x 7 H2O 0.6 g Glycerol 50.0 ml Tween 0.5 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, solid culture medium comprised of yeast extract, proteose peptone, casein peptone tyrptic digest (casitone), potassium phosphate, sodium phosphate, sodium citrate, magnesium sulfate, glycerol, tween, and agar. DSMZ Medium 218 BHI/3 medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium218.pdf 218. BHI/3 MEDIUM To medium 215 add 10 g casein hydrolysate and 1 g starch. © 2007 DSMZ GmbH - All rights reserved Carrine Blank An organic-rich, liquid culture medium comprised of brain heart infusion, casein hydrolysate, and starch. DSM strains: DSMZ Medium 216 BHI/1 medium An organic-rich, liquid culture medium comprised of brain heart infusion and yeast extract. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium216.pdf 216. BHI/1 MEDIUM To medium 215 add 2.0 g yeast extract. © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 217 An organic-rich, liquid culture medium comprised of brain heart infusion, casein hydrolysate, glucose, and yeast extract. Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium217.pdf 217. BHI/2 MEDIUM To medium 215 add 10.0 g casein hydrolysate, 5.0 g glucose and 5.0 g yeast extract. © 2007 DSMZ GmbH - All rights reserved BHI/2 medium DSMZ Medium 215b http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215b.pdf 215b. BHI MEDIUM WITH ADDITIONAL GLUCOSE Brain Heart Infusion (Difco) 37.0 g Glucose 5.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved BHI medium with additional glucose DSM strains: Carrine Blank An organic-rich, liquid culture medium comprised of brain heart infusion and glucose. DSMZ Medium 215c http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215c.pdf 215c. BHI MEDIUM FOR STRICT ANAEROBES Brain Heart Infusion (DIFCO) 37.00 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Prepare medium anoxically under 100% N2 gas atmosphere. Reducing agents are added after autoclaving from sterile anoxic solutions prepared under N2. For DSM 10643 supplement medium with 10.00 ml/l glycerol (87% w/v solution) from a sterile anoxic stock. For DSM 15692 prepare medium under 80% N2 and 20% CO2 gas atmosphere. Adjust final pH of the medium to 7.2 with a sterile anoxic solution of 10% (w/v) NaHCO3 prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 19851 supplement medium with 10.0 ml/l haemin and 0.2 ml/l vitamin K1 solution (see medium 104). © 2014 DSMZ GmbH - All rights reserved BHI medium for strict anaerobes An organic-rich, liquid culture medium comprised of brain heart infusion, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen. DSM strains: Carrine Blank DSMZ Medium 215c.3 DSMZ Medium 215c.3 -< for DSM 19851 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215c.pdf 215c. BHI MEDIUM FOR STRICT ANAEROBES Brain Heart Infusion (DIFCO) 37.00 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Prepare medium anoxically under 100% N2 gas atmosphere. Reducing agents are added after autoclaving from sterile anoxic solutions prepared under N2. For DSM 10643 supplement medium with 10.00 ml/l glycerol (87% w/v solution) from a sterile anoxic stock. For DSM 15692 prepare medium under 80% N2 and 20% CO2 gas atmosphere. Adjust final pH of the medium to 7.2 with a sterile anoxic solution of 10% (w/v) NaHCO3 prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 19851 supplement medium with 10.0 ml/l haemin and 0.2 ml/l vitamin K1 solution (see medium 104). © 2014 DSMZ GmbH - All rights reserved DSM 19851 is [Clostridium] lavalense Carrine Blank Similar to DSMZ Medium 215c, except hemin and vitamin K solution are added. soluble starch Carrine Blank starch that is soluble (having the physical quality of being dissolved) in water. activated charcoal Charcoal that has been activated by a heating process or a chemical digestion process. Carrine Blank Schaedler medium From: Schaedler Media (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Schaedler Agar is a base for several media formulations used for the recovery of anaerobic microorganisms. Principles of the Procedure The combination of three peptones derived from both animal and vegetable sources, dextrose and yeast extract render the basic formulation highly nutritious by providing nitrogenous growth factors, carbohydrates as energy sources and vitamins. The sheep blood and hemin also are important in stimulating the growth of fastidious microorganisms. As discussed above, the vitamin K1 additive is crucial for the recovery of certain anaerobes. The addition of the antimicrobial agents kanamycin and vancomycin in the agar medium renders the medium selective for gram-negative microorganisms. The kanamycin inhibits protein synthesis in susceptible organisms, whereas the vanco-mycin inhibits gram-positive bacteria by interfering with cell wall synthesis.8 Using Schaedler media, fastidious aerobes and anaerobes grow well; however, the type of organisms recovered is dependent on the environment utilized in the incubation process (aerobic, aerobic supplemented with carbon dioxide or anaerobic conditions). Formulae BBL™ Schaedler Agar Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 8.2 g Peptic Digest of Animal Tissue...................................... 2.5 g Papaic Digest of Soybean Meal..................................... 1.0 g Dextrose...................................................................... 5.8 g Yeast Extract................................................................ 5.0 g Sodium Chloride.......................................................... 1.7 g Dipotassium Phosphate................................................ 0.8 g L-Cystine...................................................................... 0.4 g Hemin.......................................................................... 0.01 g Tris (hydroxymethyl) aminomethane............................. 3.0 g Agar.......................................................................... 13.5 g BBL™ Schaedler Broth Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.6 ± 0.2 Schaedler broth An organic-rich, liquid microbiological culture medium containing peptones, dextrose, cystine, and hemin. Used for the cultivation of anaerobic heterotrophic microorganisms. Carrine Blank L-lactate alkalinization assay lLATk Assays for the ability of a microorganism to assimilate L-lactate (L-lactic acid, (S)-lactate, (S)-lactic acid) as a sole source of carbon. Assimilation of lactate results in an increase in pH (alkalinization) as a result of decarboxylation of lactate into acetate and CO2 via lactate 2-monooxygenase. lactate alkalinization L-lactate alkalinization lactate alkalinisation L-lactate alkalinisation Carrine Blank milk reactivity assay coagulate the milk An assay for the ability of a microorganism to metabolize the constituents of milk. Carrine Blank milk is coagulated Kovacs reagent A chemical solution comprised of a mixture of isoamyl alcohol, para-dimethylaminobenzaldehyde, and hydrochloric acid. Used in the indole test assay. Wikipedia: Kovac's reagent Kovac's reagent is a biochemical reagent consisting of isoamyl alcohol, para-dimethylaminobenzaldehyde and concentrated hydrochloric acid. It is used for the diagnostical indole test, to determine the ability of the organism to split indole from the amino acid tryptophan. The indole produced yields a red complex with para-dimethylaminobenzaldehyde under the given conditions.[1] Carrine Blank chymotrypsin-6 not in Chebi or in any other ontologies- Jan 2018 2-nitrophenyl beta-D-galactopyranoside-6-phosphate-7 not in Chebi - Jan 2018 - added to request list sodium aspartate-8 not in Chebi- Jan 2018 sodium glutarate-8 not in Chebi- Jan 2018 working-temp Carrine Blank DSMZ Medium 215c.2 Similar to DSMZ Medium 215c, except prepared under an atmosphere of dinitrogen and carbon dioxide. pH is increased to 7.2 with sodium bicarbonate. DSM 15692 is Atopostipes suicloacalis DSM 15692 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215c.pdf 215c. BHI MEDIUM FOR STRICT ANAEROBES Brain Heart Infusion (DIFCO) 37.00 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Prepare medium anoxically under 100% N2 gas atmosphere. Reducing agents are added after autoclaving from sterile anoxic solutions prepared under N2. For DSM 10643 supplement medium with 10.00 ml/l glycerol (87% w/v solution) from a sterile anoxic stock. For DSM 15692 prepare medium under 80% N2 and 20% CO2 gas atmosphere. Adjust final pH of the medium to 7.2 with a sterile anoxic solution of 10% (w/v) NaHCO3 prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 19851 supplement medium with 10.0 ml/l haemin and 0.2 ml/l vitamin K1 solution (see medium 104). © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 215c.2 -< for DSM 15692 Carrine Blank DSMZ Medium 215c.1 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215c.pdf 215c. BHI MEDIUM FOR STRICT ANAEROBES Brain Heart Infusion (DIFCO) 37.00 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Prepare medium anoxically under 100% N2 gas atmosphere. Reducing agents are added after autoclaving from sterile anoxic solutions prepared under N2. For DSM 10643 supplement medium with 10.00 ml/l glycerol (87% w/v solution) from a sterile anoxic stock. For DSM 15692 prepare medium under 80% N2 and 20% CO2 gas atmosphere. Adjust final pH of the medium to 7.2 with a sterile anoxic solution of 10% (w/v) NaHCO3 prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 19851 supplement medium with 10.0 ml/l haemin and 0.2 ml/l vitamin K1 solution (see medium 104). © 2014 DSMZ GmbH - All rights reserved DSM 10643 is Clostridium sp. Similar to DSMZ Medium 215c, except glycerol is added. DSMZ Medium 215c.1 -< for DSM 10643 DSMZ Medium 215a An organic-rich, liquid culture medium comprised of brain heart infusion and erythromycin. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215a.pdf 215a. BHI MEDIUM (modified) Brain heart infusion 37.0 g Erythromycin 10.0 mg Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved Carrine Blank BHI modified medium DSM strains: BHI medium (modified) DSMZ Medium 215 BHI medium Carrine Blank DSM strains: An organic-rich, liquid culture medium comprised of brain heart infusion in water. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215.pdf 215. BHI MEDIUM Brain Heart Infusion (Difco) 37.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 214 Similar to DSMZ Medium 65, except starch is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium214.pdf 214. GYM+S MEDIUM To medium 65 add 20 g starch. © 2007 DSMZ GmbH - All rights reserved GYM+S medium DSM strains: Carrine Blank DSMZ Medium 212 DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium212.pdf 212. SYNTHROPHOMONAS MEDIUM Solution A: Mineral solution (see below) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Butyric acid 1.70 g Na2SO4 2.80 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution E: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 minutes. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Note: For agar stabs add 3.00 g/l agar to the completed medium from a sterile anoxic stock solution (2% w/v). Mineral solution: KH2PO4 10.00 g MgCl2 x 6 H2O 6.60 g NaCl 8.00 g NH4Cl 8.00 g CaCl2 x 2 H2O 1.00 g Distilled water 1000.00 ml For DSM 2612A replace butyric acid by 2.00 g/l Na-benzoate. For DSM 2805 replace butyric acid by 1.50 g/l Na-propionate and supplement medium with 1.00 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. For DSM 16706 replace butyric acid by 1.50 g/l Na-propionate. For DSM 26217 omit rumen fluid and butyric acid from solution A and add to the completed medium 7.00 g/l sucrose from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Syntrophomonas medium An organic-rich, liquid culture medium comprised of mineral solution, trace elements, clarified rumen fluid, trypticase (trypticase peptone), butryic acid, sodium sulfate, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank DSMZ Medium 212.1 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium212.pdf 212. SYNTHROPHOMONAS MEDIUM Solution A: Mineral solution (see below) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Butyric acid 1.70 g Na2SO4 2.80 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution E: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 minutes. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Note: For agar stabs add 3.00 g/l agar to the completed medium from a sterile anoxic stock solution (2% w/v). Mineral solution: KH2PO4 10.00 g MgCl2 x 6 H2O 6.60 g NaCl 8.00 g NH4Cl 8.00 g CaCl2 x 2 H2O 1.00 g Distilled water 1000.00 ml For DSM 2612A replace butyric acid by 2.00 g/l Na-benzoate. For DSM 2805 replace butyric acid by 1.50 g/l Na-propionate and supplement medium with 1.00 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. For DSM 16706 replace butyric acid by 1.50 g/l Na-propionate. For DSM 26217 omit rumen fluid and butyric acid from solution A and add to the completed medium 7.00 g/l sucrose from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved DSM 2612A is Syntrophus buswellii DSMZ Medium 212.1 -< for DSM 2612A Similar to DSMZ Medium 212, except butyric acid is omitted and sodium benzoate is added. DSMZ Medium 212.2 DSMZ Medium 212.2 -< for DSM 2805 Carrine Blank Similar to DSMZ Medium 212, except butyric acid is omitted and sodium proptionate and glucose are added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium212.pdf 212. SYNTHROPHOMONAS MEDIUM Solution A: Mineral solution (see below) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Butyric acid 1.70 g Na2SO4 2.80 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution E: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 minutes. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Note: For agar stabs add 3.00 g/l agar to the completed medium from a sterile anoxic stock solution (2% w/v). Mineral solution: KH2PO4 10.00 g MgCl2 x 6 H2O 6.60 g NaCl 8.00 g NH4Cl 8.00 g CaCl2 x 2 H2O 1.00 g Distilled water 1000.00 ml For DSM 2612A replace butyric acid by 2.00 g/l Na-benzoate. For DSM 2805 replace butyric acid by 1.50 g/l Na-propionate and supplement medium with 1.00 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. For DSM 16706 replace butyric acid by 1.50 g/l Na-propionate. For DSM 26217 omit rumen fluid and butyric acid from solution A and add to the completed medium 7.00 g/l sucrose from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved DSM 2805 is Syntrophobacter wolinii DSMZ Medium 212.3 DSMZ Medium 212.3 -< for DSM 16706 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium212.pdf 212. SYNTHROPHOMONAS MEDIUM Solution A: Mineral solution (see below) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Butyric acid 1.70 g Na2SO4 2.80 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution E: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 minutes. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Note: For agar stabs add 3.00 g/l agar to the completed medium from a sterile anoxic stock solution (2% w/v). Mineral solution: KH2PO4 10.00 g MgCl2 x 6 H2O 6.60 g NaCl 8.00 g NH4Cl 8.00 g CaCl2 x 2 H2O 1.00 g Distilled water 1000.00 ml For DSM 2612A replace butyric acid by 2.00 g/l Na-benzoate. For DSM 2805 replace butyric acid by 1.50 g/l Na-propionate and supplement medium with 1.00 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. For DSM 16706 replace butyric acid by 1.50 g/l Na-propionate. For DSM 26217 omit rumen fluid and butyric acid from solution A and add to the completed medium 7.00 g/l sucrose from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 212, except butyric acid is omitted and sodium propionate is added. DSM 16706 is Syntrophobacter sulfatireducens DSMZ Medium 212.4 DSMZ Medium 212.4 -< for DSM 26217 DSM 26217 is Moorella stamsii Similar to DSMZ Medium 212, except clarified rumen fluid and butyric acid are omitted, and sucrose is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium212.pdf 212. SYNTHROPHOMONAS MEDIUM Solution A: Mineral solution (see below) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Butyric acid 1.70 g Na2SO4 2.80 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution E: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 minutes. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Note: For agar stabs add 3.00 g/l agar to the completed medium from a sterile anoxic stock solution (2% w/v). Mineral solution: KH2PO4 10.00 g MgCl2 x 6 H2O 6.60 g NaCl 8.00 g NH4Cl 8.00 g CaCl2 x 2 H2O 1.00 g Distilled water 1000.00 ml For DSM 2612A replace butyric acid by 2.00 g/l Na-benzoate. For DSM 2805 replace butyric acid by 1.50 g/l Na-propionate and supplement medium with 1.00 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. For DSM 16706 replace butyric acid by 1.50 g/l Na-propionate. For DSM 26217 omit rumen fluid and butyric acid from solution A and add to the completed medium 7.00 g/l sucrose from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 550 Similar to Czapek Dox agar, except yeast extract, casamino acids, and tryptophan are added. CYC medium DSM strains: 44065 Saccharopolyspora hordei 44575 Saccharopolyspora thermophila 43368 Thermoactinomyces vulgaris 43369 Thermoactinomyces vulgaris 43796 Thermoactinomyces vulgaris 43795 Thermobifida alba 43793 Thermobifida fusca 43028 Thermocrispum municipale 43183 Thermomonospora curvata DSM 43183 17572 Tuberibacillus calidus DSM 17572 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium550.pdf 550. CYC-MEDIUM (modified following Cross and Attwell,1973) Czapek Dox agar (Merck) 48.00 g Yeast extract (Oxoid) 2.00 g Casamino acids (Difco) 6.10 g Tryptophan 0.02 g Distilled water 1000.00 ml pH 7.2 © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 213 sulfate-free Syntrophomonas medium Carrine Blank Syntrophomonas medium (sulfate free) An organic-rich, liquid culture medium comprised of mineral solution, trace elements, clarified rumen fluid, trypticase (trypticase peptone), butyric acid, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium213.pdf 213. SYNTHROPHOMONAS MEDIUM (SULFATE FREE) Solution A: Mineral solution (see medium 212) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Butyric acid 1.70 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution E: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 minutes. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Note: For agar stabs add 3.00 g/l agar to the completed medium from a sterile anoxic stock solution (2% w/v). For DSM 2612B replace butyric acid by 2.00 g/l Na-benzoate. For DSM 2909, DSM 2984, and DSM 21899 replace butyric acid by 1.50 g/l acetoin. For DSM 15682 and DSM 16215 replace butyric acid by 1.70 g/l crotonic acid. © 2014 DSMZ GmbH - All rights reserved Syntrophomonas medium DSM strains: DSMZ Medium 213.1 Similar to DSMZ Medium 213, except butyric acid is omitted and sodium benzoate is added. DSM 2612B is Syntrophus buswellii Carrine Blank DSMZ Medium 213.1 -< for DSM 2612B http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium213.pdf 213. SYNTHROPHOMONAS MEDIUM (SULFATE FREE) Solution A: Mineral solution (see medium 212) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Butyric acid 1.70 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution E: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 minutes. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Note: For agar stabs add 3.00 g/l agar to the completed medium from a sterile anoxic stock solution (2% w/v). For DSM 2612B replace butyric acid by 2.00 g/l Na-benzoate. For DSM 2909, DSM 2984, and DSM 21899 replace butyric acid by 1.50 g/l acetoin. For DSM 15682 and DSM 16215 replace butyric acid by 1.70 g/l crotonic acid. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 213.2 DSM 2909 is Geobacter sp. DSM 2984 is Pelobacter sp. DSM 21899 is Halobacterium salinarum Carrine Blank Similar to DSMZ Medium 213, except butyric acid is omitted and acetoin added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium213.pdf 213. SYNTHROPHOMONAS MEDIUM (SULFATE FREE) Solution A: Mineral solution (see medium 212) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Butyric acid 1.70 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution E: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 minutes. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Note: For agar stabs add 3.00 g/l agar to the completed medium from a sterile anoxic stock solution (2% w/v). For DSM 2612B replace butyric acid by 2.00 g/l Na-benzoate. For DSM 2909, DSM 2984, and DSM 21899 replace butyric acid by 1.50 g/l acetoin. For DSM 15682 and DSM 16215 replace butyric acid by 1.70 g/l crotonic acid. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 213.2 -< for DSM 2909, DSM 2984, and DSM 21899 DSMZ Medium 213.3 DSMZ Medium 213.3 -< for DSM 15682 and DSM 16215 Similar to DSMZ Medium 213, except butyric acid is omitted and crotonic acid is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium213.pdf 213. SYNTHROPHOMONAS MEDIUM (SULFATE FREE) Solution A: Mineral solution (see medium 212) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Butyric acid 1.70 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution E: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 minutes. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Note: For agar stabs add 3.00 g/l agar to the completed medium from a sterile anoxic stock solution (2% w/v). For DSM 2612B replace butyric acid by 2.00 g/l Na-benzoate. For DSM 2909, DSM 2984, and DSM 21899 replace butyric acid by 1.50 g/l acetoin. For DSM 15682 and DSM 16215 replace butyric acid by 1.70 g/l crotonic acid. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSM 15682 is Syntrophomonas curvata DSM 16215 is Syntrophomonas erecta DSMZ Medium 210 An organic-rich, liquid culture medium comprised of sodium chloride, magnesium sulfate, potassium chloride, yeast extract, trypticase (trypticase peptone), vitamin solution, trace elements, thioglycolate, ascorbate, glucose, and sodium hydroxide. Prepared under an atmosphere of dinitrogen. Carrine Blank Haloanaerobium medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium210.pdf 210. HALOANAEROBIUM MEDIUM NaCl 130.0 g MgSO4 x 7 H2O 8.8 g KCl 1.0 g Yeast extract 10.0 g Trypticase (BBL) 10.0 g Vitamin solution (see medium 141) 10.0 ml Trace element solutionsee medium 144) 10.0 ml Distilled water 1000.0 ml Prepare the medium anaerobically under 100% N2. After autoclaving add from sterile solutions: Thioglycolate-ascorbate reducing agent (see below) 25.0 ml Glucose 10% (w/v) 25.0 ml 2 N NaOH 10.0 ml Final pH: 9.0. Thioglycolate-ascorbate solution: Na-thioglycolate 0.5 g Na-ascorbate 0.5 g Distilled water 25.0 ml Filter sterilize. © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 230 Tryptone yeast extract mineral medium DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium230.pdf 230. TRYPTONE YEAST EXTRACT MINERAL MEDIUM Tryptone 10.0 g Yeast extract 10.0 g K2HPO4 0.5 g KH2PO4 0.5 g NaCl 1.0 g MgSO4 0.1 g CaCl2 0.1 g NaHCO3 6.0 g Resazurin 0.1 mg Cysteine-HCl x H2O 0.3 g Arginine 3.0 g Distilled water 1000.0 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of tryptone, yeast extract, potassium phosphate, sodium chloride, magnesium sulfate, calcium chloride, sodium bicarbonate, resazurin, cyteine hydrochloride, and arginine. mineral solution for DSMZ Medium 212 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium212.pdf 212. SYNTHROPHOMONAS MEDIUM Solution A: Mineral solution (see below) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Butyric acid 1.70 g Na2SO4 2.80 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution E: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 minutes. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Note: For agar stabs add 3.00 g/l agar to the completed medium from a sterile anoxic stock solution (2% w/v). Mineral solution: KH2PO4 10.00 g MgCl2 x 6 H2O 6.60 g NaCl 8.00 g NH4Cl 8.00 g CaCl2 x 2 H2O 1.00 g Distilled water 1000.00 ml For DSM 2612A replace butyric acid by 2.00 g/l Na-benzoate. For DSM 2805 replace butyric acid by 1.50 g/l Na-propionate and supplement medium with 1.00 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. For DSM 16706 replace butyric acid by 1.50 g/l Na-propionate. For DSM 26217 omit rumen fluid and butyric acid from solution A and add to the completed medium 7.00 g/l sucrose from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Carrine Blank An inorganic salts solution comprised of potassium phosphate, magnesium chloride, sodium chloride, ammonium chloride, and calcium chloride. DSMZ Medium 243 Carrine Blank An organic-rich, solid culture medium comprised of peptone, yeast extract, agar, and filtered aged seawater or artificial sea water. seawater yeast peptone agar DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium243.pdf 243. SEAWATER YEAST PEPTONE AGAR Peptone 5.0 g Yeast extract 3.0 g Agar 12.0 g Distilled water 250.0 ml Filtered, aged seawater 750.0 ml (see below) Adjust pH to 7.8. Boil for 5 min, filter and readjust the pH to 7.3. *Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. Artificial sea water: NaCl 28.13 g KCl 0.77 g CaCl2 x 2 H2O 1.60 g MgCl2 x 6 H2O 4.80 g NaHCO3 0.11 g MgSO4 x 7 H2O 3.50 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 246 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium246.pdf 246. SEA WATER AGAR Beef extract 10.0 g Peptone 10.0 g Agar 20.0 g Tap water 250.0 ml Sea water* 750.0 ml Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121˚C for 20 min. Directly after autoclaving, add warm (55˚C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature. *Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. Artificial sea water: NaCl 28.13 g KCl 0.77 g CaCl2 x 2 H2O 1.60 g MgCl2 x 6 H2O 4.80 g NaHCO3 0.11 g MgSO4 x 7 H2O 3.50 g Distilled water © 2008 DSMZ GmbH - All rights reserved sea water agar An organic-rich, solid culture medium comprised of beef extract, peptone, agar, and filtered aged sea water or artificial sea water. DSM strains: DSMZ Medium 248 DSM strains: An organic-rich, solid culture medium comprised of filtered, boiled rolled oats (rolled oats), trace elements, yeast extract, and agar. Carrine Blank oat flakes agar with yeast extract http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium248.pdf 248. OAT FLAKES AGAR WITH YEAST EXTRACT Oat flakes 20.0 g Trace element (see medium 252) 1.0 ml Yeast extract 2.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2. Boil oat flakes for 20 min, filter through cheese cloth and adjust to 1000 ml. Add trace elements and agar. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 249 DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium249.pdf 249. OROTIC ACID MEDIUM Tryptone 5.00 g Yeast extract 0.50 g KH2PO4 1.36 g K2HPO4 6.95 g Na-orotate 2.50 g Riboflavin 15.00 mg Resazurin 1.00 mg Na-thioglycolate 0.50 g Distilled water 1000.00 ml Adjust pH to 7.5. Gas atmosphere: 100% N2. © 2007 DSMZ GmbH - All rights reserved orotic acid medium An organic-rich, liquid culture medium comprised of tryptone, yeast extract, potassium phosphate, sodium orotate, riboflavin, resazurin, and sodium thioglycolate. Prepared under an atmosphere of dinitrogen. DSMZ Medium 250 Peptone meat extract glycerol agar http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium250.pdf 250. PEPTONE MEAT EXTRACT GLYCEROL AGAR Proteose peptone no. 3 5.0 g Meat extract 3.0 g Glycerol 20.0 ml Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: An organic-rich, solid culture medium comprised of proteose peptone, meat extract, glycerol, and agar. DSMZ Medium 251 An organic-rich, solid culture medium comprised of proteose peptone, emat extract, glycerol, soil extract, and agar. Carrine Blank PFE http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium251.pdf 251. PEPTONE - MEAT EXTRACT - SOIL EXTRACT AGAR (PFE) Proteose peptone no. 3 5.0 g Meat extract 3.0 g Glycerol 20.0 g Soil extract (see medium 80) 150.0 ml Agar 15.0 g Distilled water 850.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved DSM strains: Peptone - meat extract - soil extract agar DSMZ Medium 253 DSM strains: An organic-rich, liquid culture medium comprised of potassium phosphate, calcium chloride, ammonium chloride, magnesium chloride, sodium chloride, sodium sulfate, sodium carbonate, trace elements, yeast extract, sodium succinate, sodium sulfide, vitamins, and sodium bicarbonate. Medium for Ectothiorhodospira http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium253.pdf 253. MEDIUM FOR ECTOTHIORHODOSPIRA KH2PO4 0.50 g CaCl2 x 2 H2O 0.05 g NH4Cl 0.80 g MgCl2 x 6 H2O 0.10 g NaCl 180.00 g Na2SO4 20.00 g Na2CO3 6.00 g Trace element solution (A) 1.00 ml Yeast extract 0.50 g Na-succinate 1.00 g Na2S x 9 H2O 1.00 g Distilled water 800.00 ml Adjust pH to 8.5. Sterilize at 120˚C for 15 minutes in screw-capped bottles. After sterilization add 1 ml/l filter-sterilized vitamin solution (B) and 200 ml of filter-sterilized solution (C). Trace element solution (A): FeCl2 x 4 H2O 1.80 g CoCl2 x 6 H2O 250.00 mg NiCl2 x 6 H2O 10.00 mg CuCl2 x 2 H2O 10.00 mg MnCl2 x 4 H2O 70.00 mg ZnCl2 100.00 mg H3BO3 500.00 mg Na2MoO4 x 2 H2O 30.00 mg Na2SeO3 x 5 H2O 10.00 mg Distilled water 1000.00 ml For dissolving adjust pH to about 3 with 1 N HCl. Vitamin solution (B): Biotin 10.0 mg Nicotinamide 35.0 mg Thiamine-HCl x 2 H2O 30.0 mg p-Aminobenzoic acid 20.0 mg Pyridoxal hydrochloride 10.0 mg Ca-pantothenate 10.0 mg Vitamin B12 5.0 mg Distilled water 100.0 ml Bicarbonate solution (C): NaHCO3 14.0 g H2O © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 252 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf 252. STARCH - MINERAL SALT - AGAR (STMS) Starch, soluble 10.0 g (NH4)2SO4 2.0 g K2HPO4 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g CaCO3 2.0 g Trace element solution (see medium 84) 1.0 ml Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved DSM strains: STMS Starch mineral salt agar An organic-rich, solid culture medium comprised of soluble starch, ammonium sulfate, potassium phosphate, magnesium sulfate, sodium chloride, calcium carbonate, trace elements, and agar. Starch - mineral salt - agar DSMZ Medium 255.1 Similar to DSMZ Medium 255, except cellobiose is omitted and glucose is added. DSMZ Medium 255.1 -< for DSM 2360 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium255.pdf 255. CLOSTRIDIUM (GS) MEDIUM KH2PO4 0.50 g K2HPO4 x 3 H2O 1.00 g Urea 2.00 g MgCl2 x 6 H2O 0.50 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 1.25 mg Morpholinopropane sulfonic acid 10.00 g Resazurin 0.50 mg Yeast extract 6.00 g L-Cysteine-HCl x H2O 1.00 g Cellobiose 5.00 g Distilled water 1000.00 ml Dissolve ingredients except cysteine and cellobiose. Sparge medium with 100% N2 gas for 30 – 45 min to make it anoxic, then add cysteine and adjust pH to 7.2. Dispense medium under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add cellobiose from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. For DSM 2360 replace cellobiose with 5.00 g/l D-glucose added to the medium after autoclaving from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 2360 is Ruminiclostridium thermocellum DSM 2360 DSMZ Medium 255 An organic-rich, liquid culture medium comprised of potassium phosphate, urea, magnesium chloride, calcium chloride, ferrous sulfate, morpholinopropane sulfonic acid (MES), resazurin, yeast extract, L-cysteine hydrochloride, and cellobiose. Prepared under an atmosphere of dinitrogen. Clostridium (GS) medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium255.pdf 255. CLOSTRIDIUM (GS) MEDIUM KH2PO4 0.50 g K2HPO4 x 3 H2O 1.00 g Urea 2.00 g MgCl2 x 6 H2O 0.50 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 1.25 mg Morpholinopropane sulfonic acid 10.00 g Resazurin 0.50 mg Yeast extract 6.00 g L-Cysteine-HCl x H2O 1.00 g Cellobiose 5.00 g Distilled water 1000.00 ml Dissolve ingredients except cysteine and cellobiose. Sparge medium with 100% N2 gas for 30 – 45 min to make it anoxic, then add cysteine and adjust pH to 7.2. Dispense medium under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add cellobiose from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. For DSM 2360 replace cellobiose with 5.00 g/l D-glucose added to the medium after autoclaving from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved GS medium Clostridium medium Carrine Blank DSM strains: DSMZ Medium 254 Carrine Blank An organic-rich, solid culture medium comprised of malt extract, yeast extract, agar, and ethanol. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium254.pdf 254. ACETOBACTER PEROXYDANS MEDIUM Malt extract 15.0 g Yeast extract 5.0 g Agar 15.0 g Distilled water 940.0 ml After sterilization add 60 ml ethanol (50% v/v), sterilized by filtration. © 2007 DSMZ GmbH - All rights reserved Acetobacter peroxydans medium DSMZ Medium 256 Thermus ruber medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium256.pdf 256. THERMUS RUBER MEDIUM Universal peptone (Merck) 5.0 g Yeast extract 1.0 g Starch, soluble 1.0 g Agar 12.0 g Distilled water 1000.0 ml Adjust pH to 8.0. © 2007 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, solid culture medium comprised of universal peptone (M 66), yeast extract, soluble starch, and agar. artificial sea water for DSMZ Medium 243 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium243.pdf 243. SEAWATER YEAST PEPTONE AGAR Peptone 5.0 g Yeast extract 3.0 g Agar 12.0 g Distilled water 250.0 ml Filtered, aged seawater 750.0 ml (see below) Adjust pH to 7.8. Boil for 5 min, filter and readjust the pH to 7.3. *Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. Artificial sea water: NaCl 28.13 g KCl 0.77 g CaCl2 x 2 H2O 1.60 g MgCl2 x 6 H2O 4.80 g NaHCO3 0.11 g MgSO4 x 7 H2O 3.50 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved An atificial seawater comprised of sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium bicarbonate, and magnesium sulfate. Carrine Blank filtered, boiled rolled oats filtered, boiled oat flakes Carrine Blank Boiled rolled oats that are filtered. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium248.pdf 248. OAT FLAKES AGAR WITH YEAST EXTRACT Oat flakes 20.0 g Trace element (see medium 252) 1.0 ml Yeast extract 2.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2. Boil oat flakes for 20 min, filter through cheese cloth and adjust to 1000 ml. Add trace elements and agar. © 2007 DSMZ GmbH - All rights reserved trace elements solution for DSMZ Medium 84 A trace elements solution comprised of ferrous sulfate, manganese choride, and zinc sulfate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf 84. ROLLED OATS MINERAL MEDIUM Rolled oats 20.0 g Agar 20.0 g Trace element solution (see below) 1.0 ml Distilled water 1000.0 ml Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml pH 7,2 © 2007 DSMZ GmbH - All rights reserved Carrine Blank trace elements solution A for DSMZ Medium 253 A trace elements solution comprised of ferrous chloride, cobalt chloride, nickel chloride, copper chloride, manganese chloride, zinc chloride, boric acid, sodium molybdate, and sodium selenite. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium253.pdf 253. MEDIUM FOR ECTOTHIORHODOSPIRA KH2PO4 0.50 g CaCl2 x 2 H2O 0.05 g NH4Cl 0.80 g MgCl2 x 6 H2O 0.10 g NaCl 180.00 g Na2SO4 20.00 g Na2CO3 6.00 g Trace element solution (A) 1.00 ml Yeast extract 0.50 g Na-succinate 1.00 g Na2S x 9 H2O 1.00 g Distilled water 800.00 ml Adjust pH to 8.5. Sterilize at 120˚C for 15 minutes in screw-capped bottles. After sterilization add 1 ml/l filter-sterilized vitamin solution (B) and 200 ml of filter-sterilized solution (C). Trace element solution (A): FeCl2 x 4 H2O 1.80 g CoCl2 x 6 H2O 250.00 mg NiCl2 x 6 H2O 10.00 mg CuCl2 x 2 H2O 10.00 mg MnCl2 x 4 H2O 70.00 mg ZnCl2 100.00 mg H3BO3 500.00 mg Na2MoO4 x 2 H2O 30.00 mg Na2SeO3 x 5 H2O 10.00 mg Distilled water 1000.00 ml For dissolving adjust pH to about 3 with 1 N HCl. Vitamin solution (B): Biotin 10.0 mg Nicotinamide 35.0 mg Thiamine-HCl x 2 H2O 30.0 mg p-Aminobenzoic acid 20.0 mg Pyridoxal hydrochloride 10.0 mg Ca-pantothenate 10.0 mg Vitamin B12 5.0 mg Distilled water 100.0 ml Bicarbonate solution (C): NaHCO3 14.0 g H2O © 2007 DSMZ GmbH - All rights reserved Carrine Blank vitamin solution B for DSMZ Medium 253 A vitamin solution comprised of biotin, nicotinamide, thiamine hydrochloride, p-aminobenzoic acid (4-aminobenzoic acid), pyridoxal hydrochloride, calcium pantothenate, and vitamin B12 (cobalamin). Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium253.pdf 253. MEDIUM FOR ECTOTHIORHODOSPIRA KH2PO4 0.50 g CaCl2 x 2 H2O 0.05 g NH4Cl 0.80 g MgCl2 x 6 H2O 0.10 g NaCl 180.00 g Na2SO4 20.00 g Na2CO3 6.00 g Trace element solution (A) 1.00 ml Yeast extract 0.50 g Na-succinate 1.00 g Na2S x 9 H2O 1.00 g Distilled water 800.00 ml Adjust pH to 8.5. Sterilize at 120˚C for 15 minutes in screw-capped bottles. After sterilization add 1 ml/l filter-sterilized vitamin solution (B) and 200 ml of filter-sterilized solution (C). Trace element solution (A): FeCl2 x 4 H2O 1.80 g CoCl2 x 6 H2O 250.00 mg NiCl2 x 6 H2O 10.00 mg CuCl2 x 2 H2O 10.00 mg MnCl2 x 4 H2O 70.00 mg ZnCl2 100.00 mg H3BO3 500.00 mg Na2MoO4 x 2 H2O 30.00 mg Na2SeO3 x 5 H2O 10.00 mg Distilled water 1000.00 ml For dissolving adjust pH to about 3 with 1 N HCl. Vitamin solution (B): Biotin 10.0 mg Nicotinamide 35.0 mg Thiamine-HCl x 2 H2O 30.0 mg p-Aminobenzoic acid 20.0 mg Pyridoxal hydrochloride 10.0 mg Ca-pantothenate 10.0 mg Vitamin B12 5.0 mg Distilled water 100.0 ml Bicarbonate solution (C): NaHCO3 14.0 g H2O © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 300 Similar to DSMZ Medium 298, except butanediol is omitted and sodium L-tartrate, biotin, and yeast extract are added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium300.pdf 300. RUMINOCOCCUS PASTEURII MEDIUM Use medium 298, but replace butanediol by 2.0 g/l of sodium L-tartrate. Add 0.4 mg/l biotin or 1.0 g/l of yeast extract. © 2007 DSMZ GmbH - All rights reserved Ruminococcus pasteurii medium DSM strains: Carrine Blank DSMZ Medium 301 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium301.pdf 301. FALCIVIBRIO MEDIUM Wilkins-Chalgren-Anaerobe-Broth (Oxoid) is supplemented with 0.3 g/l of L-cysteine and 1.0 mg/l of resazurin. Prepare the medium under 100% nitrogen gas. To the autoclaved medium add 50 ml/l of sterile serum (horse, bovine or lamb). © 2007 DSMZ GmbH - All rights reserved Carrine Blank Similar to Wilkins-Chalgren Anaerobe broth, except L-cysteine, rezasurin, and serum are added. Prepared under an atmosphere of dinitrogen. DSM strains: Falcivibrio medium DSMZ Medium 298b http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298b.pdf 298b. SYNTROPHOBOTULUS MEDIUM KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Na-acetate 0.20 g Resazurin 0.50 mg NaHCO3 2.50 g Na2-glyoxalate 0.60 g Vitamins solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, glyoxalate, vitamins, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add glyoxalate (sterilized by filtration), vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: When growth has started, feed culture once or twice with the same amount of glyoxalate. For DSM 6945 replace glyoxalate with 1.00 g/l Na-glycolate. © 2014 DSMZ GmbH - All rights reserved DSM strains: Syntrophobotulus medium Carrine Blank An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, trace elements, sodium acetate, resazurin, sodium bicarbonate, sodium glyoxalate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 298b.1 DSMZ Medium 298b.1 -< for DSM 6945 DSM 6945 is an unclassified bacterium. No sequences in TaxBrowser. Carrine Blank Syntrophobotulus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298b.pdf 298b. SYNTROPHOBOTULUS MEDIUM KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Na-acetate 0.20 g Resazurin 0.50 mg NaHCO3 2.50 g Na2-glyoxalate 0.60 g Vitamins solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, glyoxalate, vitamins, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add glyoxalate (sterilized by filtration), vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: When growth has started, feed culture once or twice with the same amount of glyoxalate. For DSM 6945 replace glyoxalate with 1.00 g/l Na-glycolate. © 2014 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 198b, except glyoxalate is omitted and sodium glycolate is added. DSMZ Medium 298a DSM strains: An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, yeast extract, trace elements, selenite-tungstate, resazurin, sodium bicarbonate, sodium lactate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. LuPhet1 medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298a.pdf 298a. LuPhet1 MEDIUM KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Yeast extract 1.00 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg NaHCO3 5.00 g Na-DL-lactate 1.50 g Vitamins solution (see medium 503) 1.00 ml Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, lactate, vitamins, and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic tubes or bottles under the same gas atmosphere and autoclave. Add vitamins (sterilized by filtration), lactate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 295 DSM strains: Opitutus medium An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, sodium nitrate, magnesium chloride, potassium chloride, calcium chloride, trace elements, selenite-tungstate, resazurin, sodium carbonate, D-glucose, vitamins, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium295.pdf 295. OPITUTUS MEDIUM KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g NaNO3 0.80 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Na2CO3 1.00 g D-Glucose 1.00 g Vitamins solution (see medium 141) 1.00 ml L-Cysteine-HCl x H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except carbonate, glucose, vitamins, and cysteine. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes under the same gas atmosphere and autoclave. Add vitamins, glucose and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.3 - 7.5. For DSM 11249 replace glucose with 1.00 g/l cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. © 2014 DSMZ GmbH - All rights reserved Carrine Blank Thermoproteus tenax polar lipid fraction Carrine Blank Archaea cell extract, where the archaeon is Thermofilum pendens, and the cell extract is comprised of the lipid fraction of the cell. http://www.sciencedirect.com/science/article/pii/S0723202083800356 Zillig G, Gierl A, Schreiber G, Wunderl S, Janekovic D, Stetter KO, Klenk HP. 1983. The Archaebacterium Thermofilum pendens Represents, a Novel Genus of the Thermophilic, Anaerobic Sulfur Respiring Thermoproteales. Syst Appl Microbiol 4(1):79-87. The growth of the organism requires peptides, sulfur and H2S and, in addition, a fraction of the polar lipids of the distantly related archaebacterium Thermoproteus tenax devoid of phosphate. This fraction cannot be replaced by an analogous fraction from Thermoplasma acidophilum. defibrinated blood Blood medium ingredient comprised of blood that has been definbrinated (where fibrin has been removed from the blood). Used in the cultivation of microorganisms. Carrine Blank DSMZ Medium 269.1 Carrine Blank DSMZ Medium 269.1 -< for DSM 11237 and DSM 11244 Similar to DSMZ Medium 269, except the concentration of yeast extract is decreased and the pH is increased to 3.5. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium269.pdf 269. ACIDIPHILIUM MEDIUM (NH4)2SO4 2.0 g KCl 0.1 g K2HPO4 0.5 g MgSO4 x 7 H2O 0.5 g Yeast extract 0.3 g D-Glucose 1.0 g Distilled water 1000.0 ml Autoclave glucose and yeast extract separately as a 10% and 3%, resp., solution. Adjust pH of completed medium to 3.0 with 1 N H2SO4. For DSM 11237 and DSM 11244 reduce amount of yeast extract to 0.1 g/l and adjust pH of medium to 3.5. For DSM 11245 replace yeast extract with 0.1 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 3.5. For DSM 100189 replace glucose with 1.8 g/l D-galactose and add 7.0 g/l FeSO4 x 7 H2O to the autoclaved medium from a 1 M stock solution (pH adjusted to 1.8 using 2 N H2SO4 and sterilized by filtration). Adjust pH of completed medium to 2.5. © 2015 DSMZ GmbH - All rights reserved DSM 11237 is Acidocella aminolytica 101 = DSM 11237 DSM 11244 is Acidobacterium capsulatum ATCC 51196 DSMZ Medium 269.2 Similar to DSMZ Medium 269, except yeast extract is omitted, and trypticase soy broth is added. The pH is increased to 3.5. DSM 11245 is Acidiphilium multivorum DSMZ Medium 269.2 -< for DSM 11245 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium269.pdf 269. ACIDIPHILIUM MEDIUM (NH4)2SO4 2.0 g KCl 0.1 g K2HPO4 0.5 g MgSO4 x 7 H2O 0.5 g Yeast extract 0.3 g D-Glucose 1.0 g Distilled water 1000.0 ml Autoclave glucose and yeast extract separately as a 10% and 3%, resp., solution. Adjust pH of completed medium to 3.0 with 1 N H2SO4. For DSM 11237 and DSM 11244 reduce amount of yeast extract to 0.1 g/l and adjust pH of medium to 3.5. For DSM 11245 replace yeast extract with 0.1 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 3.5. For DSM 100189 replace glucose with 1.8 g/l D-galactose and add 7.0 g/l FeSO4 x 7 H2O to the autoclaved medium from a 1 M stock solution (pH adjusted to 1.8 using 2 N H2SO4 and sterilized by filtration). Adjust pH of completed medium to 2.5. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 269.3 Similar to DSMZ Medium 269, except glucose is omitted and galactose and ferrous sulfate are added. The pH is decreased to 2.5. DSMZ Medium 269.3 -< for DSM 100189 DSM 100189 is not in www.dsmz.de or in TaxBrowser or StrainInfo. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium269.pdf 269. ACIDIPHILIUM MEDIUM (NH4)2SO4 2.0 g KCl 0.1 g K2HPO4 0.5 g MgSO4 x 7 H2O 0.5 g Yeast extract 0.3 g D-Glucose 1.0 g Distilled water 1000.0 ml Autoclave glucose and yeast extract separately as a 10% and 3%, resp., solution. Adjust pH of completed medium to 3.0 with 1 N H2SO4. For DSM 11237 and DSM 11244 reduce amount of yeast extract to 0.1 g/l and adjust pH of medium to 3.5. For DSM 11245 replace yeast extract with 0.1 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 3.5. For DSM 100189 replace glucose with 1.8 g/l D-galactose and add 7.0 g/l FeSO4 x 7 H2O to the autoclaved medium from a 1 M stock solution (pH adjusted to 1.8 using 2 N H2SO4 and sterilized by filtration). Adjust pH of completed medium to 2.5. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 269 Acidiphilum medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium269.pdf 269. ACIDIPHILIUM MEDIUM (NH4)2SO4 2.0 g KCl 0.1 g K2HPO4 0.5 g MgSO4 x 7 H2O 0.5 g Yeast extract 0.3 g D-Glucose 1.0 g Distilled water 1000.0 ml Autoclave glucose and yeast extract separately as a 10% and 3%, resp., solution. Adjust pH of completed medium to 3.0 with 1 N H2SO4. For DSM 11237 and DSM 11244 reduce amount of yeast extract to 0.1 g/l and adjust pH of medium to 3.5. For DSM 11245 replace yeast extract with 0.1 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 3.5. For DSM 100189 replace glucose with 1.8 g/l D-galactose and add 7.0 g/l FeSO4 x 7 H2O to the autoclaved medium from a 1 M stock solution (pH adjusted to 1.8 using 2 N H2SO4 and sterilized by filtration). Adjust pH of completed medium to 2.5. © 2015 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium chloride, potassium phosphate, magnesium sulfate, yeast extract, and D-glucose. Carrine Blank DSMZ Medium 265 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium265.pdf 265. THERMOFILUM PENDENS MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 x 7 H2O 0.01 mg Yeast extract 2.00 g Sucrose 2.00 g Sulfur, powdered 10.00 g Polar lipid fraction prepared from Thermoproteus tenax (DSM 2078) or from any other archaebacterium, aqueous suspension 6 - 12.00 ml Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Adjust final pH to 5.2. Prepare the medium anaerobically under 100% nitrogen. The following constituents are prepared separately and added to the autoclaved mineral salt solution: Yeast extract (20 ml of 10% w/v solution)-boiled for few minutes, not autoclaved; sucrose (20 ml of 10% w/v solution)-filter-sterilized; sulfur (10 g)-sterilized by steaming for 3 h on each of three successive days; polar lipid fraction (6 - 12ml)-prepared as described by W. Zillig et al. (1983), Syst. Appl. Microbiol. 4: 79 - 87; Na2S x 9 H2O (10 ml of 3% w/v solution)-autoclaved under nitrogen atmosphere. © 2007 DSMZ GmbH - All rights reserved Thermofilum pendens medium An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric trichloride, manganese chloride, sodium tetraborate, zinc sulfate, copper chloride, sodium molybdate, vanadyl sulfate, cobalt sulfate, yeast extract, sucrose, elemental sulfur, sodium sulfide, and the polar lipid fraction of Thermoproteus tenax. Prepared under an atmosphere of dinitrogen. Carrine Blank DSM strains: DSMZ Medium 266 DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium266.pdf 266. THERMOCOCCUS CELER MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 x 7 H2O 0.01 mg NaCl 40.00 g Resazurin 1.00 mg Yeast extract 2.00 g Sulfur, powdered 5.00 g Distilled water 1000.00 ml Adjust final pH to 5.8. Prepare the medium anaerobically under 100% nitrogen. The following constituents are prepared separately and added to the autoclaved mineral salt solution: Yeast extract (20 ml of 10% w/v solution)-boiled for a few minutes not autoclaved; sulfur (10 g)-sterilized by steaming for 3 h on each of three successive days; Na2S x 9 H2O (10 ml of 3% w/v solution)-autoclaved under nitrogen atmosphere. © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric trichloride, manganese chloride, sodium tetraborate, zinc sulfte, copper chloride, sodium molybdate, vanadyl sulfate, cobalt sulfate, sodium choride, resazurin, yeast extract, and elemental sulfur. Prepared under an atmosphere of dinitrogen. Thermococcus celer medium Carrine Blank DSMZ Medium 264 An organic-rich, liquid culture medium comprised of casein peptone, tryptic digested (casitone), yeast extract, filtered tomato juice, and Tween 80 (polysorbate 80). Tomato juice medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium264.pdf 264. TOMATO JUICE MEDIUM Casein peptone, tryptic digested 10.0 g Yeast extract 10.0 g Filtered tomato juice 200.0 ml (pH 7.0) Tween 80 1.0 ml Distilled water 800.0 ml Adjust pH to 6.5. © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 262 J-agar Carrine Blank An organic-rich, liquid culture medium comprised of tryptone, yeast extract, glucose, potassium phosphate, and agar. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium262.pdf 262. J - AGAR Tryptone 5.0 g Yeast extract 15.0 g Glucose 2.0 g K2HPO4 3.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.3 - 7.5. Sterilize glucose separately and add after sterilization. © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 295.1 DSMZ Medium 295.1 -< for DSM 11249 Carrine Blank DSM 11249 is Opitutus terrae Similar to DSMZ Medium 295, except glucose is omitted and cellobiose is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium295.pdf 295. OPITUTUS MEDIUM KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g NaNO3 0.80 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Na2CO3 1.00 g D-Glucose 1.00 g Vitamins solution (see medium 141) 1.00 ml L-Cysteine-HCl x H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except carbonate, glucose, vitamins, and cysteine. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes under the same gas atmosphere and autoclave. Add vitamins, glucose and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.3 - 7.5. For DSM 11249 replace glucose with 1.00 g/l cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 261 Medium for chemilithotrophic growth of Bacillus schlegelii DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium261.pdf 261. MEDIUM FOR CHEMOLITHOTROPHIC GROWTH OF BACILLUS SCHLEGELII Use medium 260 without pyruvate. Incubate at 65˚C without agitation under 5% (v/v) O2, 10% CO2, 45% H2. © 2007 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 260, except sodium pyruvate is omitted. Prepared under an atmosphere of dioxygen, carbon dioxide, and dihydrogen. DSMZ Medium 259 Carrine Blank Yeast extract mineral medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium259.pdf 259. YEAST EXTRACT MINERAL MEDIUM Na2HPO4 x 12 H2O 3.50 g K2HPO4 1.00 g MgSO4 x 7 H2O 0.03 g NH4Cl 0.50 g Yeast extract 4.00 g Agar 15.00 g Distilled water 1000.00 ml Adjust pH to 7.0 - 7.2. © 2007 DSMZ GmbH - All rights reserved An organic-rich, solid culture medium comprised of sodium phosphate, potassium phosphate, magnesium sulfate, ammonium chloride, yeast extract, and agar. DSMZ Medium 260 An organic-rich, liquid culture medium comprised of sodium phosphate, potassium phosphate, ammonium chloride, manganese sulfate, magnesium sulfate, calcium chloride, ferric ammonium citrate, trace elements, and sodium pyruvate. Carrine Blank Bacillus schlegelii heterotrophic medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium260.pdf 260. BACILLUS SCHLEGELII HETEROTROPHIC MEDIUM Na2HPO4 x 2 H2O 4.50 g KH2PO4 1.50 g NH4Cl 1.00 g MnSO4 x H2O 0.01 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.01 g Ferric ammonium citrate 5.00 mg Trace element solution SL-6 3.00 ml (see medium 27) Na-Pyruvate 1.50 g Distilled water 1000.00 ml Adjust pH to 7.1. Agar if necessary 15.0 g. Distribute in 30 - 50 ml amounts in Erlenmeyer flasks and sterilize for 15 minutes at 121˚C. Incubate without agitation at 65˚C. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 238 Similar to DSMZ Medium 1, except streptomycin sulfate is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium238.pdf 238. STREPTOMYCIN NUTRIENT AGAR To medium 1 add 40 mg/l streptomycin sulfate. © 2007 DSMZ GmbH - All rights reserved Streptomycin nutrient agar DSM strains: Carrine Blank DSMZ Medium 239 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium239.pdf 239. DIETHYL PHOSPHONATE MEDIUM KCl 0.20 g MgSO4 x 7 H2O 0.20 g NH4Cl 0.20 g Tris(hydroxymethyl)methylamine 6.00 g p-Hydroxybenzoate, Na-salt 0.75 g Agar 12.00 g Distilled water 900.00 ml Adjust pH to 7.4. After sterilization add 100 ml of a filter-sterilized solution of 1 mM diethyl phosphonate (Eastman Organic Chemical Div., Rochester, N.Y., USA) or of 20 mM phosphate buffer. © 2007 DSMZ GmbH - All rights reserved DSM strains: Diethyl phosphonate medium A minerals-salts, solid culture medium comprised of potassium chloride, magnesium sulfate, ammonium chloride, tris(hydroxymethyl)methylamine (tris), sodium p-hydroxybenzoate (sodium 4-hydroxybenzoate), agar, and diethyl phosphonate or a potassium or sodium phosphate buffer solution. Carrine Blank DSMZ Medium 242 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium242.pdf 242. BMM - MEDIUM (NH4)2SO4 3.0 g KH2PO4 2.0 g K2HPO4 7.0 g NaCl 2.0 g MgSO4 x 7 H2O 0.5 g Yeast extract 0.1 g Biotin 10.0 μg Thiamine-HCl x 2 H2O 100.0 μg MnSO4 x H2O 6.2 mg FeSO4 x 7 H2O 10.0 mg Methanol 10.0 ml Agar (if necessary) 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2 prior to autoclaving. © 2007 DSMZ GmbH - All rights reserved DSM strains: BMM medium An organic-rich, solid culture medium comprised of ammonium sulfate, potassium phosphate, sodium chloride, magnesium sulfate, yeast extract, biotin, thiamine hydrochloride, manganese sulfate, methanol, and agar. DSMZ Medium 240 Similar to DSMZ Medium 53, except defibrinated blood is added. Corynebacterium medium with blood. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium240.pdf 240. CORYNEBACTERIUM MEDIUM WITH BLOOD Sterilize medium 53 and cool to about 45˚C. Aseptically add 5% defibrinated blood. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank DSMZ Medium 245 Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium245.pdf 245. BLOOD AGAR II Medium 220 supplemented with 5% defibrinated sheep blood. © 2007 DSMZ GmbH - All rights reserved Blood agar II Similar to DSMZ Medium 220, except defibrinated sheep blood is added. Universal Peptone M 66 universal peptone (Merck) From: http://www.emdmillipore.com/US/en/product/Universal-peptone-M-66,MDA_CHEM-107043#documentation (See Universal Peptone M66 document under Supporting Documentation). Mode of Action Universal M 66 peptone is a mixture of casein peptone and meat peptone. Universal peptone M 66 combines the nutritive characteristics of casein and meat peptone. The well balanced peptone mix containing a representative mix of low and high molecular peptones, a broad range of free amino acids in growth supporting concentrations, vitamins and other growth factors. Universal peptone M 66 can be used as nutrient, for example as replacement of serum albumin, in cell culture and in fermentations. As an ingredient in culture media Universal Peptone M66 can be employed in media for the cultivation of a wide range of fastidious bacteria. A microbiological medium ingredient, derived from enzymatic hydrolysis of mammalian protein. A mixture of casein peptone (casitone) and meat peptone. Carrine Blank DSMZ Medium 298 DSM strains: Pelobacter propionicus freshwater medium Pelobacter propionicus medium (freshwater) Carrine Blank An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, trace elements, resazurin, sodium bicarbonate, 2,3-butanediol (butane-2,3-diol), and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 298.1 Similar to DSMZ Medium 298, except butanediol is omitted and poly(ethylene glycol) is added. DSMZ Medium 298.1 -< for DSM 2395 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved DSM 2395 is Scytalidium lignicola Carrine Blank DSMZ Medium 298.2 DSM 2612M is Syntrophus buswellii Similar to DSMZ Medium 298, except butanediol is omitted and seven vitamins solution and crotonic acid are added. DSMZ Medium 298.2 -< for DSM 2612M Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 298.3 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 298.3 -< for DSM 2805M DSM 2805M is Syntrophobacter wolinii Similar to DSMZ Medium 298, except butanediol is omitted and seven vitamins solution and sodium pyruvate are added. DSMZ Medium 298.4 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 3246 is Pelobacter acetylenicus Similar to DSMZ Medium 298, except butanediol is omitted and acetoin is added. DSMZ Medium 298.4 -< for DSM 3246 DSMZ Medium 298.5 Carrine Blank Similar to DSMZ Medium 298, except butanediol is omitted and sodium glutarate and clarified rumen fluid are added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved DSM 6652 is Pelospora glutarica DSMZ Medium 298.5 -< for DSM 6652 DSMZ Medium 298.6 DSMZ Medium 298.6 -< for DSM 6832 Similar to DSMZ Medium 298, except butanediol is omitted and vitamins and quinic acid are added. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved DSM 6832 is Propionivibrio limicola DSMZ Medium 298.7 Carrine Blank DSM 8385 is Clostridium sp. DSMZ Medium 298.7 -< for DSM 8385 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 298, except butanediol is omitted and yeast extract and D-galactose are added. DSMZ Medium 298.8 DSMZ Medium 298.8 -< for DSM 8909 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 298, except butanediol is omitted and seven vitamins solution and triethanolamine hydrochloride are added. DSM 8909 is Acetobacterium (no sequences in TaxBrowser) Carrine Blank DSMZ Medium 298.9 DSM 11264 is Geobacter hephaestius http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 298, except butanediol is omitted and yeast extract, vitamins and ethylene glycol are added. DSMZ Medium 298.9 -< for DSM 11264 DSMZ Medium 298.10 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 298, except vitamins and selenite-tungstate are added. DSM 17541 is anaerobic syntrophic bacterium NE23-3 DSMZ Medium 298.10 -< for DSM 17541 DSMZ Medium 298.11 DSMZ Medium 298.11 -< for DSM 29698 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 29698 is Clostridiales bacterium GluBS11 Similar to DSMZ Medium 298, except butanediol is omitted and sodium gluconate is added. DSMZ Medium 294 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium294.pdf 294. HQGö1 MEDIUM KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 1.00 g Agar (for semi-solid media) 2.70 g Resazurin 0.50 mg NaHCO3 2.50 g Vitamin solution (see medium 503) 1.00 ml Gentisic acid 0.30 g Na2S x 9 H2O 0.10 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, gentisic acid and sulfide), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Dispense under same gas atmosphere in culture vessels and autoclave. Add vitamins, gentisic acid (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. If necessary, adjust the final pH of the medium to 7.2 with a bicarbonate stock solution. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. © 2014 DSMZ GmbH - All rights reserved DSM strains: HQGö1 medium HQGo1 medium An organic-rich, solid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, trace elements, selenite-tungstate, yeast extract, agar, resazurin, sodium bicarbonate, vitamins, gentisic acid (2,5-dihydroxybenzoic acid), and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank DSMZ Medium 293 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium293.pdf 293. PROPIONIGENIUM MODESTUM MEDIUM (MARINE) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 20.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g Na2-succinate 3.25 g Na2S x 9 H2O 0.36 g Distilled Water 1000.00 ml Dissolve ingredients except bicarbonate, succinate and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes under the same gas atmosphere and autoclave. Add succinate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. For DSM 2376 supplement medium with 0.10% yeast extract (separately sterilized). For DSM 2377 and DSM 2378 replace Na2-succinate with 0.68 g/l of gallic acid. For DSM 2380 replace Na2-succinate with 0.90 g/l of 2,3-butanediol. For DSM 2382 replace Na2-succinate with 2.00 g/l of sodium L-tartrate. For DSM 2394 replace Na2-succinate with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 3247 replace Na2-succinate with 1.00 g/l acetoin. For DSM 6831 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace Na2-succinate with 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, trace elements, resazurin, sodium bicarbonate, sodium succinate, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Propionigenium modestum marine medium Propionigenium modestum medium (marine) DSMZ Medium 293.1 DSM 2376 is Propionigenium modestum http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium293.pdf 293. PROPIONIGENIUM MODESTUM MEDIUM (MARINE) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 20.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g Na2-succinate 3.25 g Na2S x 9 H2O 0.36 g Distilled Water 1000.00 ml Dissolve ingredients except bicarbonate, succinate and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes under the same gas atmosphere and autoclave. Add succinate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. For DSM 2376 supplement medium with 0.10% yeast extract (separately sterilized). For DSM 2377 and DSM 2378 replace Na2-succinate with 0.68 g/l of gallic acid. For DSM 2380 replace Na2-succinate with 0.90 g/l of 2,3-butanediol. For DSM 2382 replace Na2-succinate with 2.00 g/l of sodium L-tartrate. For DSM 2394 replace Na2-succinate with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 3247 replace Na2-succinate with 1.00 g/l acetoin. For DSM 6831 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace Na2-succinate with 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 293, except yeast extract is added. Carrine Blank DSMZ Medium 293.1 -< for DSM 2376 DSMZ Medium 293.2 Similar to DSMZ Medium 293, except sodium succinate is omitted and gallic acid is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium293.pdf 293. PROPIONIGENIUM MODESTUM MEDIUM (MARINE) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 20.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g Na2-succinate 3.25 g Na2S x 9 H2O 0.36 g Distilled Water 1000.00 ml Dissolve ingredients except bicarbonate, succinate and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes under the same gas atmosphere and autoclave. Add succinate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. For DSM 2376 supplement medium with 0.10% yeast extract (separately sterilized). For DSM 2377 and DSM 2378 replace Na2-succinate with 0.68 g/l of gallic acid. For DSM 2380 replace Na2-succinate with 0.90 g/l of 2,3-butanediol. For DSM 2382 replace Na2-succinate with 2.00 g/l of sodium L-tartrate. For DSM 2394 replace Na2-succinate with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 3247 replace Na2-succinate with 1.00 g/l acetoin. For DSM 6831 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace Na2-succinate with 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved DSM 2377 is Pelobacter acidigallici DSM 2378 is Pelobacter acidigallici Carrine Blank DSMZ Medium 293.2 -< for DSM 2377 and DSM 2378 DSMZ Medium 293.3 DSMZ Medium 293.3 -< for DSM 2380 DSM 2380 is Pelobacter carbinolicus DSM 2380 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium293.pdf 293. PROPIONIGENIUM MODESTUM MEDIUM (MARINE) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 20.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g Na2-succinate 3.25 g Na2S x 9 H2O 0.36 g Distilled Water 1000.00 ml Dissolve ingredients except bicarbonate, succinate and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes under the same gas atmosphere and autoclave. Add succinate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. For DSM 2376 supplement medium with 0.10% yeast extract (separately sterilized). For DSM 2377 and DSM 2378 replace Na2-succinate with 0.68 g/l of gallic acid. For DSM 2380 replace Na2-succinate with 0.90 g/l of 2,3-butanediol. For DSM 2382 replace Na2-succinate with 2.00 g/l of sodium L-tartrate. For DSM 2394 replace Na2-succinate with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 3247 replace Na2-succinate with 1.00 g/l acetoin. For DSM 6831 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace Na2-succinate with 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 293, except sodium succinate is omitted and 2,3-butanediol (butane-2,3-diol) is added. DSMZ Medium 293.4 DSM 2382 is Ilyobacter tartaricus DSMZ Medium 293.4 -< for DSM 2382 Carrine Blank Similar to DSMZ Medium 293, except sodium succinate is omitted and sodium L-tartrate is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium293.pdf 293. PROPIONIGENIUM MODESTUM MEDIUM (MARINE) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 20.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g Na2-succinate 3.25 g Na2S x 9 H2O 0.36 g Distilled Water 1000.00 ml Dissolve ingredients except bicarbonate, succinate and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes under the same gas atmosphere and autoclave. Add succinate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. For DSM 2376 supplement medium with 0.10% yeast extract (separately sterilized). For DSM 2377 and DSM 2378 replace Na2-succinate with 0.68 g/l of gallic acid. For DSM 2380 replace Na2-succinate with 0.90 g/l of 2,3-butanediol. For DSM 2382 replace Na2-succinate with 2.00 g/l of sodium L-tartrate. For DSM 2394 replace Na2-succinate with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 3247 replace Na2-succinate with 1.00 g/l acetoin. For DSM 6831 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace Na2-succinate with 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 293.5 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium293.pdf 293. PROPIONIGENIUM MODESTUM MEDIUM (MARINE) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 20.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g Na2-succinate 3.25 g Na2S x 9 H2O 0.36 g Distilled Water 1000.00 ml Dissolve ingredients except bicarbonate, succinate and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes under the same gas atmosphere and autoclave. Add succinate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. For DSM 2376 supplement medium with 0.10% yeast extract (separately sterilized). For DSM 2377 and DSM 2378 replace Na2-succinate with 0.68 g/l of gallic acid. For DSM 2380 replace Na2-succinate with 0.90 g/l of 2,3-butanediol. For DSM 2382 replace Na2-succinate with 2.00 g/l of sodium L-tartrate. For DSM 2394 replace Na2-succinate with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 3247 replace Na2-succinate with 1.00 g/l acetoin. For DSM 6831 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace Na2-succinate with 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 293, except sodium succinate is omitted and polyethylene glycol (poly(ethylene glycol)) is added. DSM 2394 is Pelobacter venetianus DSMZ Medium 293.5 -< for DSM 2394 DSMZ Medium 293.6 Similar to DSMZ Medium 293, except sodium succinate is omitted and acetoin is added. Carrine Blank DSMZ Medium 293.6 -< for DSM 3247 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium293.pdf 293. PROPIONIGENIUM MODESTUM MEDIUM (MARINE) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 20.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g Na2-succinate 3.25 g Na2S x 9 H2O 0.36 g Distilled Water 1000.00 ml Dissolve ingredients except bicarbonate, succinate and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes under the same gas atmosphere and autoclave. Add succinate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. For DSM 2376 supplement medium with 0.10% yeast extract (separately sterilized). For DSM 2377 and DSM 2378 replace Na2-succinate with 0.68 g/l of gallic acid. For DSM 2380 replace Na2-succinate with 0.90 g/l of 2,3-butanediol. For DSM 2382 replace Na2-succinate with 2.00 g/l of sodium L-tartrate. For DSM 2394 replace Na2-succinate with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 3247 replace Na2-succinate with 1.00 g/l acetoin. For DSM 6831 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace Na2-succinate with 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved DSM 3247 is Pelobacter acetylenicus DSMZ Medium 293.7 DSM 6831 is Ilyobacter insuetus DSMZ Medium 293.7 -< for DSM 6831 Similar to DSMZ Medium 293, except sodium succinate is omitted and vitamins and quinic acid are added. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium293.pdf 293. PROPIONIGENIUM MODESTUM MEDIUM (MARINE) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 20.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g Na2-succinate 3.25 g Na2S x 9 H2O 0.36 g Distilled Water 1000.00 ml Dissolve ingredients except bicarbonate, succinate and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes under the same gas atmosphere and autoclave. Add succinate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. For DSM 2376 supplement medium with 0.10% yeast extract (separately sterilized). For DSM 2377 and DSM 2378 replace Na2-succinate with 0.68 g/l of gallic acid. For DSM 2380 replace Na2-succinate with 0.90 g/l of 2,3-butanediol. For DSM 2382 replace Na2-succinate with 2.00 g/l of sodium L-tartrate. For DSM 2394 replace Na2-succinate with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 3247 replace Na2-succinate with 1.00 g/l acetoin. For DSM 6831 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace Na2-succinate with 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 292 Acidaminobacter medium DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium292.pdf 292. ACIDAMINOBACTER MEDIUM Glycine 1.50 g Na2SO4 3.00 g KH2PO4 0.20 g MgCl2 x 6 H2O 0.40 g NaCl 1.20 g CaCl2 x 2 H2O 0.15 g KCl 0.40 g Yeast extract 0.20 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg NaHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins and sulfide), adjust pH to 6.5, then sparge with 100% N2 gas for 30 – 45 min to make it anoxic and dispense under same gas atmosphere in anoxic tubes or bottles. After autoclaving complete the medium by adding vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are sterilized by filtration. Adjust pH of the completed medium to 7.4 - 7.5. © 2015 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of glycine, sodium sulfate, potassium phosphate, magnesium chloride, sodium chloride, calcium chloride, potassium chloride, yeast extract, trace elements, selenite-tungstate, resazurin, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 291 Pyrobaculum neutrophilum medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium291.pdf 291. PYROBACULUM NEUTROPHILUM MEDIUM Prepare medium 88 without yeast extract, then add per liter: Sulfur, powdered 8.0 g Resazurin 0.4 mg Adjust pH to 6.5 with NaOH, bring to boil for 5 min, then cool to room temperature under H2 + CO2 (80% + 20%) gas mixture. Add 0.85 g NaHCO3 per liter and equilibrate the pH to c. 6.5 by further gassing. Dispense in serum bottles (10 ml/30 ml bottle), seal and heat the bottles for one hour at 85˚C on each of three successive days. Before inoculation, add from anaerobic stock solutions 0.02% yeast extract (for mixotrophic growth only) and 0.01 ml Na-dithionite solution (250 mg Na-dithionite/10 ml dist. water, filter-sterilized). After inoculation, pressurize to 1 bar H2 + CO2 overpressure. © 2007 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 88, except elemental sulfur, resazurin, sodium hydroxide, sodium dithionite and sodium bicarbonate are added. The pH is is increased to 6.5. Prepared under an atmosphere of dihydrogen and carbon dioxide. Carrine Blank DSMZ Medium 289 An organic-rich, liquid culture medium comprised of filtered sea water, mineral salt solution, ammonium chloride, yeast extract, potassium phosphate, resazurin, L-cysteine hydrochloride, and cellobiose. Prepared under an atmosphere of dinitrogen. DSM strains: Clostridium papyrosolvens medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium289.pdf 289. CLOSTRIDIUM PAPYROSOLVENS MEDIUM Sea water, filtered 200.00 ml Mineral salt solution (see below) 150.00 ml NH4Cl 1.00 g Yeast extract 0.60 g K2HPO4 1.65 g Resazurin 0.50 mg L-Cysteine-HCl x H2O 0.50 g Cellobiose 5.00 g Distilled water 650.00 ml Dissolve ingredients (except cysteine and cellobiose), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere and add cysteine. Adjust pH to 7.2 and dispense under 100% N2 gas in culture vessels and autoclave. After sterilization add cellobiose from an anoxic stock solution sterilized by filtration. Note: Some strains can be adapted to cellulose as substrate using 5.0 g/l cellulose (Lens tissue, OXOID; or cellulose powder MN 301, MACHEREY-NAGEL). If necessary adjust pH of completed medium to 7.2. Mineral salt solution: (NH4)2SO4 6.00 g NaCl 6.00 g MgSO4 x 7 H2O 1.20 g CaCl2 x 2 H2O 0.80 g Distilled water 1000.00 ml © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 287 An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium sulfate, sodium chloride, calcium chloride, magnesium sulfate, trace elements, ammonium chloride, sodium acetate, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium287.pdf 287. METHANOGENIUM OLENTANGYI MEDIUM K2HPO4 0.30 g KH2PO4 0.30 g (NH4)2SO4 0.30 g NaCl 0.61 g CaCl2 x H2O 0.14 g MgSO4 x 7 H2O 0.13 g Trace element solution (see medium 141) 10.00 ml NH4Cl 2.70 g Na-acetate 2.50 g Resazurin 0.50 mg NaHCO3 5.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, adjust pH to 6.8 and then dispense medium in anoxic Hungatetype tubes or serum bottles under 80% H2 and 20% CO2 gas atmosphere and autoclave. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at one atmospheres of pressure. © 2014 DSMZ GmbH - All rights reserved Methanogenium olentangyi medium Carrine Blank DSMZ Medium 288 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium288.pdf 288. METHANOCOCCUS DELTAE MEDIUM K2HPO4 0.30 g KH2PO4 0.30 g (NH4)2SO4 0.30 g NaCl 35.00 g MgCl2 x 6 H2O 4.00 g CaCl2 x H2O 0.14 g Trace element solution (see medium 141) 10.00 ml NH4Cl 2.70 g Na-acetate 2.50 g Resazurin 0.50 mg NaHCO3 3.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, adjust pH to 6.8 and then dispense medium in anoxic Hungatetype tubes or serum bottles under 80% H2 and 20% CO2 gas atmosphere and autoclave. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at one atmospheres of pressure. © 2014 DSMZ GmbH - All rights reserved DSM strains: Methanococcus deltae medium Carrine Blank An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium sulfate, sodium chloride, magnesium chloride, calcium chloride, trace elements, ammonium chloride, sodium acetate, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen. DSMZ Medium 286 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium286.pdf 286. CLOSTRIDIUM AMINOBUTYRICUM MEDIUM KH2PO4 1.30 g K2HPO4 7.00 g MgCl2 x 6 H2O 0.20 g FeCl3 x 6 H2O 0.01 g CaCl2 x 2 H2O 0.01 g Na2MoO4 x 2 H2O 1.00 mg Yeast extract 3.00 g gamma-Aminobutyric acid 5.00 g Agar 1.50 g Resazurin 0.50 mg Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except sulfide), adjust pH to 7.2, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under N2. © 2014 DSMZ GmbH - All rights reserved Carrine Blank An organic-rich, solid culture medium comprised of potassium phosphate, magnesium chloride, ferric trichloride, calcium chloride, sodium molybdate, yeast extract, gamma-aminobutyric acid, agar, resazurin, and sodium sulfide. Prepared under an atmosphere of dinitrogen. DSM strains: Clostridium aminobutyricum medium DSMZ Medium 284 An organic-rich, liquid culture medium comprised of potassium phosphate, calcium chloride, ammonium chloride, magnesium sulfate, magnesium chloride, potassium chloride, nickel chloride, sodium selenite, sodium chloride, ferrous ammonium sulfate, trace elements, resazurin, sodium bicarbonate, yeast extract, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: Kosmotoga medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium284.pdf 284. KOSMOTOGA MEDIUM K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g NH4Cl 0.25 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.10 g KCl 0.33 g NiCl2 x 6 H2O 0.50 mg Na2SeO3 x 5 H2O 0.50 mg NaCl 30.00 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml Resazurin 0.50 mg NaHCO3 2.50 g Yeast extract 1.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, yeast extract, vitamins, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under same gas atmosphere in anoxic Hungate-type tubes and autoclave. Add vitamins (sterilized by filtration), yeast extract, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use check pH of completed medium and adjust to 7.0 – 7.2, if necessary. After inoculation add sterile 80% N2 and 20% CO2 gas mixture to 1 bar overpressure. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 283 Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium283.pdf 283. PYRODICTIUM MEDIUM NaCl 13.85 g MgSO4 x 7 H2O 3.50 g MgCl2 x 6 H2O 2.75 g KCl 0.33 g NaBr 0.05 g H3BO3 15.00 mg SrCl2 x 6 H2O 7.50 mg (NH4)2SO4 10.00 mg Citric acid 5.00 mg KI 0.05 mg CaCl2 x 2 H2O 0.75 g KH2PO4 0.50 g NiCl2 x 6 H2O 2.00 mg Trace elements (see medium 141) 10.00 ml Yeast extract 2.00 g Sulfur, powdered 30.00 g Resazurin 0.50 mg Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Prepare the medium without sodium sulfide and adjust pH to 5.5 with 10 N sulfuric acid, boil and then cool under a stream of 80% H2 and 20% CO2 gas atmosphere. Add sodium sulfide, adjust the pH to 5.5, and distribute in appropriate vessels under 80% H2 and CO2 gas atmosphere, thereby taking care to transfer also the necessary amount of sulfur. Heat the vessels containing the medium in boiling water for 1 hour before inoculation. For storage of medium at room temperature heat vessels at least on 2 successive days. Do not autoclave! After inoculation pressurize the vessels to 200 - 300 kPa overpressure with 80% H2 and 20% CO2 gas mixture. For DSM 2709 reduce amount of yeast extract to 0.20 g/l. For DSM 6158 reduce amount of yeast extract to 0.50 g/l and omit citric acid. © 2015 DSMZ GmbH - All rights reserved Pyrodictium medium An organic-rich, liquid culture medium comprised of sodium chloride, magnesium sulfate, magnesium chloride, potassium chloride, sodium bromide, boric acid, strontium chloride, ammonium sulfate, citric acid, potassium iodide, calcium chloride, potassium phosphate, nickel chloride, trace elements, yeast extract, elemental sulfur, resazurin, and sodium sulfide. Prepared under an atmosphere of dihydrogen and carbon dioxide. DSMZ Medium 283.1 Similar to DSMZ Medium 283, except the concentration of yeast extract is decreased. DSM 2709 is Pyrodictium occultum Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium283.pdf 283. PYRODICTIUM MEDIUM NaCl 13.85 g MgSO4 x 7 H2O 3.50 g MgCl2 x 6 H2O 2.75 g KCl 0.33 g NaBr 0.05 g H3BO3 15.00 mg SrCl2 x 6 H2O 7.50 mg (NH4)2SO4 10.00 mg Citric acid 5.00 mg KI 0.05 mg CaCl2 x 2 H2O 0.75 g KH2PO4 0.50 g NiCl2 x 6 H2O 2.00 mg Trace elements (see medium 141) 10.00 ml Yeast extract 2.00 g Sulfur, powdered 30.00 g Resazurin 0.50 mg Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Prepare the medium without sodium sulfide and adjust pH to 5.5 with 10 N sulfuric acid, boil and then cool under a stream of 80% H2 and 20% CO2 gas atmosphere. Add sodium sulfide, adjust the pH to 5.5, and distribute in appropriate vessels under 80% H2 and CO2 gas atmosphere, thereby taking care to transfer also the necessary amount of sulfur. Heat the vessels containing the medium in boiling water for 1 hour before inoculation. For storage of medium at room temperature heat vessels at least on 2 successive days. Do not autoclave! After inoculation pressurize the vessels to 200 - 300 kPa overpressure with 80% H2 and 20% CO2 gas mixture. For DSM 2709 reduce amount of yeast extract to 0.20 g/l. For DSM 6158 reduce amount of yeast extract to 0.50 g/l and omit citric acid. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 283.1 -< for DSM 2709 DSMZ Medium 283.2 DSMZ Medium 283.2 -< for DSM 6158 Carrine Blank Similar to DSMZ Medium 283, except the concentration of yeast extract is decreased and citric acid is omitted. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium283.pdf 283. PYRODICTIUM MEDIUM NaCl 13.85 g MgSO4 x 7 H2O 3.50 g MgCl2 x 6 H2O 2.75 g KCl 0.33 g NaBr 0.05 g H3BO3 15.00 mg SrCl2 x 6 H2O 7.50 mg (NH4)2SO4 10.00 mg Citric acid 5.00 mg KI 0.05 mg CaCl2 x 2 H2O 0.75 g KH2PO4 0.50 g NiCl2 x 6 H2O 2.00 mg Trace elements (see medium 141) 10.00 ml Yeast extract 2.00 g Sulfur, powdered 30.00 g Resazurin 0.50 mg Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Prepare the medium without sodium sulfide and adjust pH to 5.5 with 10 N sulfuric acid, boil and then cool under a stream of 80% H2 and 20% CO2 gas atmosphere. Add sodium sulfide, adjust the pH to 5.5, and distribute in appropriate vessels under 80% H2 and CO2 gas atmosphere, thereby taking care to transfer also the necessary amount of sulfur. Heat the vessels containing the medium in boiling water for 1 hour before inoculation. For storage of medium at room temperature heat vessels at least on 2 successive days. Do not autoclave! After inoculation pressurize the vessels to 200 - 300 kPa overpressure with 80% H2 and 20% CO2 gas mixture. For DSM 2709 reduce amount of yeast extract to 0.20 g/l. For DSM 6158 reduce amount of yeast extract to 0.50 g/l and omit citric acid. © 2015 DSMZ GmbH - All rights reserved DSM 6158 is Pyrodictium abyssi DSM 6158 DSMZ Medium 282a DSM strains: A minerals-salts, liquid culture medium comprised of potassium phosphate, calcium chloride, ammonium chloride, magnesium sulfate, magnesium chloride, potassium chloride, nickel chloride, sodium chloride, ferrous ammonium sulfate, trace elements, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium282a.pdf 282a. METHANOCALDOCOCCUS VILLOSUS MEDIUM K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g NH4Cl 0.25 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.10 g KCl 0.33 g NiCl2 x 6 H2O 0.50 mg NaCl 18.00 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml Resazurin 0.50 mg NaHCO3 1.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 6.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. © 2015 DSMZ GmbH - All rights reserved Methanocaldococcus villosus medium Carrine Blank DSMZ Medium 282 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium282.pdf 282. METHANOCALDOCOCCUS MEDIUM K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g NH4Cl 0.25 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.10 g KCl 0.33 g NiCl2 x 6 H2O 0.50 mg NaCl 30.00 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml Resazurin 0.50 mg NaHCO3 1.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 6.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Note: Some strains grow within a few hours to stationary phase, thus check growth in regular intervals and do not incubate over night. For DSM 5666 adjust pH of completed medium to 6.5. For DSM 16983 adjust pH to 7.0 – 7.2. For DSM 26965 supplement medium with 3.00 g/l yeast extract and add 2.50 g/l maltose to the autoclaved medium from a sterile anoxic stock solution prepared under 100% N2 gas. Adjust pH of completed medium to 6.5 and use without overpressure of sterile 80% H2 and 20% CO2 gas mixture. For DSM 27223 adjust pH of completed medium to 7.0. © 2015 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank A minerals-salts, liquid culture medium comprised of potassium phosphate, calcium chloride, ammonium chloride, magnesium sulfate, magnesium chloride, potassium chloride, nickel chloride, sodium chloride, ferrous ammonium sulfate, trace elements, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen. Methanocaldococcus medium DSMZ Medium 282.1 Carrine Blank Similar to DSMZ Medium 282, except the pH is increased to 6.5. DSM 5666 is Methanotorris igneus Kol 5 DSMZ Medium 282.1 -< for DSM 5666 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium282.pdf 282. METHANOCALDOCOCCUS MEDIUM K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g NH4Cl 0.25 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.10 g KCl 0.33 g NiCl2 x 6 H2O 0.50 mg NaCl 30.00 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml Resazurin 0.50 mg NaHCO3 1.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 6.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Note: Some strains grow within a few hours to stationary phase, thus check growth in regular intervals and do not incubate over night. For DSM 5666 adjust pH of completed medium to 6.5. For DSM 16983 adjust pH to 7.0 – 7.2. For DSM 26965 supplement medium with 3.00 g/l yeast extract and add 2.50 g/l maltose to the autoclaved medium from a sterile anoxic stock solution prepared under 100% N2 gas. Adjust pH of completed medium to 6.5 and use without overpressure of sterile 80% H2 and 20% CO2 gas mixture. For DSM 27223 adjust pH of completed medium to 7.0. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 282.2 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium282.pdf 282. METHANOCALDOCOCCUS MEDIUM K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g NH4Cl 0.25 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.10 g KCl 0.33 g NiCl2 x 6 H2O 0.50 mg NaCl 30.00 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml Resazurin 0.50 mg NaHCO3 1.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 6.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Note: Some strains grow within a few hours to stationary phase, thus check growth in regular intervals and do not incubate over night. For DSM 5666 adjust pH of completed medium to 6.5. For DSM 16983 adjust pH to 7.0 – 7.2. For DSM 26965 supplement medium with 3.00 g/l yeast extract and add 2.50 g/l maltose to the autoclaved medium from a sterile anoxic stock solution prepared under 100% N2 gas. Adjust pH of completed medium to 6.5 and use without overpressure of sterile 80% H2 and 20% CO2 gas mixture. For DSM 27223 adjust pH of completed medium to 7.0. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 282.2 -< for DSM 16983 DSM 16983 is Methanotorris formicicus Mc-S-70 Similar to DSMZ Medium 282, except the pH is increased to 7.0-7.2. DSMZ Medium 282.3 DSMZ Medium 282.3 -< for DSM 26965 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium282.pdf 282. METHANOCALDOCOCCUS MEDIUM K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g NH4Cl 0.25 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.10 g KCl 0.33 g NiCl2 x 6 H2O 0.50 mg NaCl 30.00 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml Resazurin 0.50 mg NaHCO3 1.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 6.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Note: Some strains grow within a few hours to stationary phase, thus check growth in regular intervals and do not incubate over night. For DSM 5666 adjust pH of completed medium to 6.5. For DSM 16983 adjust pH to 7.0 – 7.2. For DSM 26965 supplement medium with 3.00 g/l yeast extract and add 2.50 g/l maltose to the autoclaved medium from a sterile anoxic stock solution prepared under 100% N2 gas. Adjust pH of completed medium to 6.5 and use without overpressure of sterile 80% H2 and 20% CO2 gas mixture. For DSM 27223 adjust pH of completed medium to 7.0. © 2015 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 282, except yeast extract and maltose are added. The pH is increased to 6.5. DSM 26965 is Kosmotoga pacifica DSMZ Medium 282.4 Similar to DSMZ Medium 282, except the pH is increased to 7.0. DSMZ Medium 282.4 -< for DSM 27223 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium282.pdf 282. METHANOCALDOCOCCUS MEDIUM K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g NH4Cl 0.25 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.10 g KCl 0.33 g NiCl2 x 6 H2O 0.50 mg NaCl 30.00 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml Resazurin 0.50 mg NaHCO3 1.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 6.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Note: Some strains grow within a few hours to stationary phase, thus check growth in regular intervals and do not incubate over night. For DSM 5666 adjust pH of completed medium to 6.5. For DSM 16983 adjust pH to 7.0 – 7.2. For DSM 26965 supplement medium with 3.00 g/l yeast extract and add 2.50 g/l maltose to the autoclaved medium from a sterile anoxic stock solution prepared under 100% N2 gas. Adjust pH of completed medium to 6.5 and use without overpressure of sterile 80% H2 and 20% CO2 gas mixture. For DSM 27223 adjust pH of completed medium to 7.0. © 2015 DSMZ GmbH - All rights reserved DSM 27223 is Methanocaldococcus bathoardescens DSMZ Medium 281 Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium281.pdf 281. HYPHOMONAS MEDIUM Casitone (Difco) 2.0 g Yeast extract (Difco) 1.0 g MgCl2 x 6 H2O 2.0 g Distilled water 1000.0 ml Adjust pH to 7.5 (use indicator paper). © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of casitone, yeast extract, and magnesium chloride. Hyphomonas medium DSMZ Medium 280.4 Similar to DSMZ Medium 280, except trimethylamine is omitted and methanol added. DSMZ Medium 280.4 -< for DSM 14042 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium280.pdf 280. METHANOCOCCOIDES MEDIUM (N2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase (BD BBL) 2.00 g Resazurin 0.50 mg NaHCO3 5.00 g Trimethylamine x HCl 3.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, trimethylamine, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add trimethylamine, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 7.0 - 7.2. For DSM 2831 replace trimethylamine with 5.00 g/l sucrose added from a sterile anoxic stock solution prepared under N2. For DSM 4138 replace trimethylamine with 5.00 g/l D-glucose added from a sterile anoxic stock solution prepared under N2. Use an overpressure of 2 bar of 80% N2 and 20% CO2 gas mixture. For DSM 11916 adjust pH to 6.6. For DSM 14042 replace trimethylamine with 0.50 ml/l methanol added from a sterile anoxic stock solution prepared under N2 gas. For incubation use 80% N2 and 20% CO2 gas mixture at two atmospheres of pressure. For DSM 16625 supplement the autoclaved medium with 3.00 g/l Na2S2O3 from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved DSM 14042 is Methanosarcina baltica GS1-A DSMZ Medium 280.3 Carrine Blank DSM 11916 is Methanosarcina siciliae C2J DSMZ Medium 280.3 -< for DSM 11916 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium280.pdf 280. METHANOCOCCOIDES MEDIUM (N2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase (BD BBL) 2.00 g Resazurin 0.50 mg NaHCO3 5.00 g Trimethylamine x HCl 3.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, trimethylamine, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add trimethylamine, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 7.0 - 7.2. For DSM 2831 replace trimethylamine with 5.00 g/l sucrose added from a sterile anoxic stock solution prepared under N2. For DSM 4138 replace trimethylamine with 5.00 g/l D-glucose added from a sterile anoxic stock solution prepared under N2. Use an overpressure of 2 bar of 80% N2 and 20% CO2 gas mixture. For DSM 11916 adjust pH to 6.6. For DSM 14042 replace trimethylamine with 0.50 ml/l methanol added from a sterile anoxic stock solution prepared under N2 gas. For incubation use 80% N2 and 20% CO2 gas mixture at two atmospheres of pressure. For DSM 16625 supplement the autoclaved medium with 3.00 g/l Na2S2O3 from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 280, except the pH is decreased to 6.6. DSMZ Medium 280.2 DSMZ Medium 280.2 -< for DSM 4138 DSM 4138 is Thermotoga http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium280.pdf 280. METHANOCOCCOIDES MEDIUM (N2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase (BD BBL) 2.00 g Resazurin 0.50 mg NaHCO3 5.00 g Trimethylamine x HCl 3.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, trimethylamine, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add trimethylamine, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 7.0 - 7.2. For DSM 2831 replace trimethylamine with 5.00 g/l sucrose added from a sterile anoxic stock solution prepared under N2. For DSM 4138 replace trimethylamine with 5.00 g/l D-glucose added from a sterile anoxic stock solution prepared under N2. Use an overpressure of 2 bar of 80% N2 and 20% CO2 gas mixture. For DSM 11916 adjust pH to 6.6. For DSM 14042 replace trimethylamine with 0.50 ml/l methanol added from a sterile anoxic stock solution prepared under N2 gas. For incubation use 80% N2 and 20% CO2 gas mixture at two atmospheres of pressure. For DSM 16625 supplement the autoclaved medium with 3.00 g/l Na2S2O3 from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 280, except trimethylamine is omitted and glucose is added. The partial pressures of dinitrogen and carbon dioxide are increased. DSMZ Medium 280.1 Carrine Blank DSMZ Medium 280.1 -< for DSM 2831 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium280.pdf 280. METHANOCOCCOIDES MEDIUM (N2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase (BD BBL) 2.00 g Resazurin 0.50 mg NaHCO3 5.00 g Trimethylamine x HCl 3.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, trimethylamine, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add trimethylamine, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 7.0 - 7.2. For DSM 2831 replace trimethylamine with 5.00 g/l sucrose added from a sterile anoxic stock solution prepared under N2. For DSM 4138 replace trimethylamine with 5.00 g/l D-glucose added from a sterile anoxic stock solution prepared under N2. Use an overpressure of 2 bar of 80% N2 and 20% CO2 gas mixture. For DSM 11916 adjust pH to 6.6. For DSM 14042 replace trimethylamine with 0.50 ml/l methanol added from a sterile anoxic stock solution prepared under N2 gas. For incubation use 80% N2 and 20% CO2 gas mixture at two atmospheres of pressure. For DSM 16625 supplement the autoclaved medium with 3.00 g/l Na2S2O3 from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 280, except trimethylamine is omitted and sucrose is added. DSM 2831 is not in TaxBrowser or StrainInfo. DSMZ Medium 280.5 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium280.pdf 280. METHANOCOCCOIDES MEDIUM (N2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase (BD BBL) 2.00 g Resazurin 0.50 mg NaHCO3 5.00 g Trimethylamine x HCl 3.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, trimethylamine, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add trimethylamine, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 7.0 - 7.2. For DSM 2831 replace trimethylamine with 5.00 g/l sucrose added from a sterile anoxic stock solution prepared under N2. For DSM 4138 replace trimethylamine with 5.00 g/l D-glucose added from a sterile anoxic stock solution prepared under N2. Use an overpressure of 2 bar of 80% N2 and 20% CO2 gas mixture. For DSM 11916 adjust pH to 6.6. For DSM 14042 replace trimethylamine with 0.50 ml/l methanol added from a sterile anoxic stock solution prepared under N2 gas. For incubation use 80% N2 and 20% CO2 gas mixture at two atmospheres of pressure. For DSM 16625 supplement the autoclaved medium with 3.00 g/l Na2S2O3 from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 280, except sodium thiosulfate is added. DSMZ Medium 280.5 -< for DSM 16625 DSM 16625 is Methanococcoides methylutens MM1 DSMZ Medium 280 Methanococcoides medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium280.pdf 280. METHANOCOCCOIDES MEDIUM (N2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase (BD BBL) 2.00 g Resazurin 0.50 mg NaHCO3 5.00 g Trimethylamine x HCl 3.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, trimethylamine, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add trimethylamine, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 7.0 - 7.2. For DSM 2831 replace trimethylamine with 5.00 g/l sucrose added from a sterile anoxic stock solution prepared under N2. For DSM 4138 replace trimethylamine with 5.00 g/l D-glucose added from a sterile anoxic stock solution prepared under N2. Use an overpressure of 2 bar of 80% N2 and 20% CO2 gas mixture. For DSM 11916 adjust pH to 6.6. For DSM 14042 replace trimethylamine with 0.50 ml/l methanol added from a sterile anoxic stock solution prepared under N2 gas. For incubation use 80% N2 and 20% CO2 gas mixture at two atmospheres of pressure. For DSM 16625 supplement the autoclaved medium with 3.00 g/l Na2S2O3 from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved Methanococcoides medium (N2/CO2) An organic-rich, liquid culture medium comprised of potassium chloride, magnesium chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, sodium acetate, yeast extract, trypticase (trypticase peptone), resazurin, sodium bicarbonate, trimethylamine hydrochloride, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: Carrine Blank DSMZ Medium 277 Carrine Blank Cherry agar An organic-rich, solid culture medium comprised of sour stone cherry extract and agar. CBS formula http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium277.pdf 277. CHERRY AGAR (CBS formula) Add 1 l water to 200.0 g pulp of sour stone cherries. Heat to boiling and simmer gently for 2 h. Strain through cloth and sterilize at 110˚C for 30 minutes. Dissolve 20.0 g agar in 800 ml water and sterilize. Add 200 ml cherry extract, distribute in presterilized tubes and sterilize for 5 min at 120˚C. Final pH 3.8 - 4.6. © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 276 Halomonas medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium276.pdf 276. HALOMONAS MEDIUM NaCl 80.00 g Casamino acids (with vitamins) 7.50 g Proteose peptone No. 3 5.00 g Yeast extract 1.00 g Na3-citrate 3.00 g MgSO4 x 7 H2O 20.00 g K2HPO4 0.50 g Fe(NH4)2(SO4)2 x 6 H2O 0.05 g Agar, if necessary 15.00 g Distilled water 1000.00 ml Before autoclaving adjust pH to 7.5 with KOH. © 2007 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, solid culture medium comprised of sodium chloride, casamino acids, proteose peptone, yeast extract, sodium citrate, magnesium sulfate, potassium phosphate, potassium phosphate, ferrous ammonium citrate, and agar. Carrine Blank DSMZ Medium 275 DSM strains: Carrine Blank An organic-rich, liquid culture medium comprised of calcium chloride, magnesium sulfate, potassium phosphate, sodium chloride, rumen fluid, yeast extract, peptone, ammonium sulfate, resazurin, cysteine hydrochloride, glucose, and sodium bicarbonate. Prepared under an atmosphere of dinitrogen. Treponema succinifaciens medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium275.pdf 275. TREPONEMA SUCCINIFACIENS MEDIUM Solution A: CaCl2 x 2 H2O 0.100 g MgSO4 x 7 H2O 0.100 g KH2PO4 0.500 g K2HPO4 0.500 g NaCl 1.000 g Rumen fluid (clarified) 300.000 ml Yeast extract (Difco) 0.500 g Peptone (Difco) 0.500 g (NH4)2SO4 0.500 g Resazurin 0.001 g Cysteine-HCl x H2O 0.500 g Distilled water 575.000 ml Solution B: Glucose (20% w/v, heat-sterilized) 50.000 ml Solution C: Potassium phosphate buffer (1M, pH 7.4, heat-sterilized) 25.000 ml Solution D: NaHCO3 (5% w/v, filter-sterilized) 50.000 ml Final pH is 7.4. Prepare the medium anaerobically under 100% nitrogen atmosphere. Dissolve ingredients of part A, in the amount of water indicated, adjust pH to 6.2 - 6.3 with 4N HCl and heat to boiling point. Cool to room temperature under 100% nitrogen and distribute under the same gas in anaerobic tubes. Autoclave 20 minutes at 121˚C. After the broth had cooled, add appropriate amounts each of part B, C, and D, (all solutions prepared under nitrogen) to the tubes. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 274 An organic-rich, liquid culture medium comprised of potassium chloride, magnesium chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, sodium bicarbonate, sodium acetate, resazurin, MOPS, vitamin solution, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen and carbon dioxide. DSM strains: Carrine Blank Methanomicrobium paynteri medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium274.pdf 274. METHANOMICROBIUM PAYNTERI MEDIUM KCl 0.34 g MgCl2 x 2 H2O 2.75 g MgSO4 x 7 H2O 3.40 g NH4Cl 1.50 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 6.31 g Trace element solution (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg NaHCO3 5.00 g Na-acetate x 3 H2O 4.15 g Resazurin 0.50 mg MOPS (3-(N-morpholino) propane-sulfonic acid) 20.93 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except MOPS, vitamins, cysteine and sulfide in a total volume of 890 ml. The MOPS buffer is dissolved separately in 90 ml of distilled water, the pH is adjusted to 7.0 with 2 M KOH, then add the buffer to the salt solution. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense medium in anoxic Hungate-type tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 7.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 273 Spirochaeta isovalerica medium DSM strains: An organic-rich, liquid culture medium comprised of glucose, trypticase (trypticase peptone), yeast extract, tris, sea water, cysteine hydrochloride, and resazurin. Prepared under an atmosphere of dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium273.pdf 273. SPIROCHAETA ISOVALERICA MEDIUM Glucose 2.000 g Trypticase (BBL) 1.000 g Yeast extract 0.500 g Tris-HCl-buffer (0.2 M; pH 7.5) 250.000 ml Sea water 750.000 ml Cysteine-HCl x H2O 0.050 g Resazurin 0.001 g Adjust pH to 7.5. Prepare the medium anaerobically under 100% nitrogen atmosphere. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 271 Acidithiobacillus ferrooxidans medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium271.pdf 271. ACIDITHIOBACILLUS FERROOXIDANS (APH) MEDIUM (NH4)2SO4 2.00 g K2HPO4 0.50 g MgSO4 x 7 H2O 0.50 g KCl 0.10 g Ca(NO3)2 0.01 g FeSO4 x 7 H2O 8.00 g Distilled water 1000.00 ml Dissolve ingredients and adjust pH to 2.0 with 10 N H2SO4. Sterilize the medium by filtration. Alternatively, autoclave separately the basal medium (pH adjusted to 2.0) and the ferrous sulfate (8.00 g FeSO4 x 7 H2O in 50 ml distilled water, pH not adjusted, in a sealed vessel under nitrogen gas atmosphere). For DSM 11478 and DSM 24413: Omit the ferrous sulfate from the medium and add 10.00 g/l elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100˚C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. Adjust pH of completed medium to 2.0 for DSM 11478 and to 2.5 for DSM 24413. © 2015 DSMZ GmbH - All rights reserved Acidithiobacillus ferrooxidans (APH) medium DSM strains: Carrine Blank APH medium A minerals-salts, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, potassium chloride, calcium nitrate, and ferrous sulfate. DSMZ Medium 271.1 Similar to DSMZ Medium 271, except ferrous sulfate is omitted and elemental sulfur is added. Carrine Blank DSMZ Medium 271.1 -< for DSM 11478 and DSM 24413 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium271.pdf 271. ACIDITHIOBACILLUS FERROOXIDANS (APH) MEDIUM (NH4)2SO4 2.00 g K2HPO4 0.50 g MgSO4 x 7 H2O 0.50 g KCl 0.10 g Ca(NO3)2 0.01 g FeSO4 x 7 H2O 8.00 g Distilled water 1000.00 ml Dissolve ingredients and adjust pH to 2.0 with 10 N H2SO4. Sterilize the medium by filtration. Alternatively, autoclave separately the basal medium (pH adjusted to 2.0) and the ferrous sulfate (8.00 g FeSO4 x 7 H2O in 50 ml distilled water, pH not adjusted, in a sealed vessel under nitrogen gas atmosphere). For DSM 11478 and DSM 24413: Omit the ferrous sulfate from the medium and add 10.00 g/l elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100˚C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. Adjust pH of completed medium to 2.0 for DSM 11478 and to 2.5 for DSM 24413. © 2015 DSMZ GmbH - All rights reserved DSM 11478 is Acidithiobacillus thiooxidans DSM 24413 is Acidithiobacillus caldus cherry extract Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium277.pdf 277. CHERRY AGAR (CBS formula) Add 1 l water to 200.0 g pulp of sour stone cherries. Heat to boiling and simmer gently for 2 h. Strain through cloth and sterilize at 110˚C for 30 minutes. Dissolve 20.0 g agar in 800 ml water and sterilize. Add 200 ml cherry extract, distribute in presterilized tubes and sterilize for 5 min at 120˚C. Final pH 3.8 - 4.6. © 2007 DSMZ GmbH - All rights reserved An extract of sour stone cherries (Prunus cerasus). Produces by heating in water, then straining and sterilizing the extract. Wilkins-Chalgren Anaerobe Broth Carrine Blank http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0643&cat=&sec=1&c=UK&lang=EN WILKINS-CHALGREN ANAEROBE BROTH Code: CM0643 A medium for the general growth and antimicrobial susceptibility testing of anaerobic organisms. See Antimicrobial Susceptibility Testing Section 6 for details of the use of this medium in AST methodology. Typical Formula* gm/litre Tryptone 10.0 Gelatin peptone 10.0 Yeast extract 5.0 Glucose 1.0 Sodium chloride 5.0 L-Arginine 1.0 Sodium pyruvate 1.0 Menadione 0.0005 Haemin 0.005 pH 7.1 ± 0.2 @ 25°C * Adjusted as required to meet performance standards Directions Suspend 33g in 1 litre of warm distilled water. Mix well, distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes. Description Wilkins-Chalgren Anaerobe Broth is derived from Wilkins-Chalgren Anaerobe Agar1 CM0619. Where studies on antimicrobial susceptibilities are being made both in broth and agar, standardisation is improved by using media of identical nutrient formulation. The growth of anaerobic organisms in this broth is particularly good. The formulation includes yeast extract to supply vitamins and other growth factors such as purines and pyrimidines, that are necessary for good growth of Peptostreptococcus anaerobius and Bacteroides melaninogenicus. Arginine is added to ensure sufficient amino-acid is available for the growth of Eubacterium lentum. Pyruvate is present as an energy source for asaccharolytic cocci such as Veillonella. It also acts similarly to catalase and degrades traces of hydrogen peroxide, which may be produced by the action of molecular oxygen on medium constituents and interfere with the metabolism of anaerobes. Haemin is found to be essential for the growth of Bacteroides species and menadione for Bacteroides melaninogenicus. Peptones derived from the single protein sources casein and gelatin are used to improve standardisation of the medium. The early heavy growth that is usual may reflect the absence of Eh-reducing substances that can be inhibitory to some organisms. An organic-rich, liquid culture medium comprised of tryptone, gelatin peptone (pancreatic digest of gelatin), yeast extract, glucose, sodium chloride, L-arginine, sodium pyruvate, menadione, and hemin. mineral salt solution for DSMZ Medium 289 An inorganic salts solution comprised of ammonium sulfate, sodium chloride, magnesium sulfate, and calcium chloride. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium289.pdf 289. CLOSTRIDIUM PAPYROSOLVENS MEDIUM Sea water, filtered 200.00 ml Mineral salt solution (see below) 150.00 ml NH4Cl 1.00 g Yeast extract 0.60 g K2HPO4 1.65 g Resazurin 0.50 mg L-Cysteine-HCl x H2O 0.50 g Cellobiose 5.00 g Distilled water 650.00 ml Dissolve ingredients (except cysteine and cellobiose), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere and add cysteine. Adjust pH to 7.2 and dispense under 100% N2 gas in culture vessels and autoclave. After sterilization add cellobiose from an anoxic stock solution sterilized by filtration. Note: Some strains can be adapted to cellulose as substrate using 5.0 g/l cellulose (Lens tissue, OXOID; or cellulose powder MN 301, MACHEREY-NAGEL). If necessary adjust pH of completed medium to 7.2. Mineral salt solution: (NH4)2SO4 6.00 g NaCl 6.00 g MgSO4 x 7 H2O 1.20 g CaCl2 x 2 H2O 0.80 g Distilled water 1000.00 ml © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 329 Methanohalophilus halophilus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium329.pdf 329. METHANOHALOPHILUS HALOPHILUS MEDIUM NH4Cl 0.33 g KH2PO4 0.33 g KCl 0.33 g K2SO4 0.01 g NaCl 70.00 g Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 0.05 g Resazurin 0.50 mg NaHCO3 5.00 g MgCl2 x 6 H2O 0.33 g CaCl2 x 2 H2O 0.33 g Vitamin solution (see medium 141) 10.00 ml Trimethylamine-HCl 5.00 g L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.15 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, magnesium chloride, calcium chloride, vitamins, trimethylamine, cysteine, and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add bicarbonate and equilibrate pH to 6.9 - 7.0, then distribute under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add magnesium chloride, calcium chloride, vitamins (sterilized by filtration), trimethylamine, cysteine, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use adjust pH of completed medium to 7.2. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, potassium chloride, potassium sulfate, sodium chloride, trace elements, yeast extract, resazurin, sodium bicarbonate, magnesium chloride, calcium chloride, vitamins, trimethylamine hydrochloride, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 328 DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium328.pdf 328. PYG MEDIUM WITH VOLATILE FATTY ACIDS Beef extract 5.00 g Trypticase (BBL) 5.00 g Yeast extract 10.00 g Mineral solution (see below) 40.00 ml (NH4)2SO4 0.50 g Fatty acid mixture (see below) 3.10 ml Glucose 5.00 g Resazurin 1.00 mg Haemin (dissolved in 0.5 ml 1 N NaOH) 5.00 mg Vitamin K1 (0.5% sol. in ethanol) 0.20 ml Cysteine-HCl x H2O 0.50 g Distilled water 960.00 ml Adjust pH to 6.9. Dissolve ingredients except cysteine-HCl in water, boil for 5 min, cool to room temperature while gassing with 100% CO2, add cysteine-HCl and adjust pH to 6.9 with 8 N NaOH while gassing is continued. Switch to 100% N2 gas after pH has reached the desired value, distribute in anaerobic tubes under nitrogen, and autoclave. Mineral solution: CaCl2 x 2 H2O 0.30 g MgSO4 x 7 H2O 0.48 g K2HPO4 1.00 g KH2PO4 1.00 g NaHCO3 10.00 g NaCl 2.00 g Distilled water 1000.00 ml Fatty acid mixture: Acetic acid 17.0 ml Propionic acid 6.0 ml n-Butyric acid 4.0 ml n-Valeric acid 1.0 ml iso-Valeric acid 1.0 ml iso-Butyric acid 1.0 ml DL-2-Methylbutyric acid 1.0 ml © 2007 DSMZ GmbH - All rights reserved PYG medium with volatile fatty acids An organic-rich, liquid culture medium comprised of beef extract, trypticase (trypticase peptone), yeast extract, mineral solution, ammonium sulfate, fatty acid mixture, glucose, resazurin, hemin, vitamin K1, and cysteine hydrochloride. Prepared under an atmosphere of dinitrogen and carbon dioxide. mineral solution for DSMZ Medium 328 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium328.pdf 328. PYG MEDIUM WITH VOLATILE FATTY ACIDS Beef extract 5.00 g Trypticase (BBL) 5.00 g Yeast extract 10.00 g Mineral solution (see below) 40.00 ml (NH4)2SO4 0.50 g Fatty acid mixture (see below) 3.10 ml Glucose 5.00 g Resazurin 1.00 mg Haemin (dissolved in 0.5 ml 1 N NaOH) 5.00 mg Vitamin K1 (0.5% sol. in ethanol) 0.20 ml Cysteine-HCl x H2O 0.50 g Distilled water 960.00 ml Adjust pH to 6.9. Dissolve ingredients except cysteine-HCl in water, boil for 5 min, cool to room temperature while gassing with 100% CO2, add cysteine-HCl and adjust pH to 6.9 with 8 N NaOH while gassing is continued. Switch to 100% N2 gas after pH has reached the desired value, distribute in anaerobic tubes under nitrogen, and autoclave. Mineral solution: CaCl2 x 2 H2O 0.30 g MgSO4 x 7 H2O 0.48 g K2HPO4 1.00 g KH2PO4 1.00 g NaHCO3 10.00 g NaCl 2.00 g Distilled water 1000.00 ml Fatty acid mixture: Acetic acid 17.0 ml Propionic acid 6.0 ml n-Butyric acid 4.0 ml n-Valeric acid 1.0 ml iso-Valeric acid 1.0 ml iso-Butyric acid 1.0 ml DL-2-Methylbutyric acid 1.0 ml © 2007 DSMZ GmbH - All rights reserved Carrine Blank An inorganic salts solution comprised of calcium chloride, magnesium sulfate, potassium phosphate, sodium bicarbonate, and sodium chloride. fatty acid mixture for DSMZ Medium 328 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium328.pdf 328. PYG MEDIUM WITH VOLATILE FATTY ACIDS Beef extract 5.00 g Trypticase (BBL) 5.00 g Yeast extract 10.00 g Mineral solution (see below) 40.00 ml (NH4)2SO4 0.50 g Fatty acid mixture (see below) 3.10 ml Glucose 5.00 g Resazurin 1.00 mg Haemin (dissolved in 0.5 ml 1 N NaOH) 5.00 mg Vitamin K1 (0.5% sol. in ethanol) 0.20 ml Cysteine-HCl x H2O 0.50 g Distilled water 960.00 ml Adjust pH to 6.9. Dissolve ingredients except cysteine-HCl in water, boil for 5 min, cool to room temperature while gassing with 100% CO2, add cysteine-HCl and adjust pH to 6.9 with 8 N NaOH while gassing is continued. Switch to 100% N2 gas after pH has reached the desired value, distribute in anaerobic tubes under nitrogen, and autoclave. Mineral solution: CaCl2 x 2 H2O 0.30 g MgSO4 x 7 H2O 0.48 g K2HPO4 1.00 g KH2PO4 1.00 g NaHCO3 10.00 g NaCl 2.00 g Distilled water 1000.00 ml Fatty acid mixture: Acetic acid 17.0 ml Propionic acid 6.0 ml n-Butyric acid 4.0 ml n-Valeric acid 1.0 ml iso-Valeric acid 1.0 ml iso-Butyric acid 1.0 ml DL-2-Methylbutyric acid 1.0 ml © 2007 DSMZ GmbH - All rights reserved Carrine Blank A fatty acid solution comprised of acetic acid, propionic acid, butyric acid, valeric acid, iso-valeric acid, iso-butyric acid, and 2-methylbutyric acid. DSMZ Medium 309 Neomycin agar http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium309.pdf 309. NEOMYCIN AGAR Beef extract 1.5 g Yeast extract 3.0 g Peptone 6.0 g Glucose 1.0 g Casitone 4.0 g Distilled water 1000.0 ml Adjust pH to 7.0. Aseptically add neomycin to a final concentration of 1.0 mg/ml of medium. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank An organic-rich, liquid culture medium comprised of beef extract, yeast extract, peptone, glucose, casitone, and neomycin. DSMZ Medium 306 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium306.pdf 306. NY-AGAR To medium 1 add 0.5% yeast extract. © 2007 DSMZ GmbH - All rights reserved DSM strains: Similar to DSMZ Medium 1, except yeast extract is added. Carrine Blank NY-agar DSMZ Medium 308 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium308.pdf 308. VIBRIO MEDIUM Tryptone 10.0 g NaCl 10.0 g MgCl2 x 6 H2O 4.0 g KCl 1.0 g Distilled water 1000.0 ml Adjust pH to 7.5. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank Vibrio medium An organic-rich, liquid culture medium comprised of tryptone, sodium chloride, magnesium chloride, and potassium chloride. DSMZ Medium 305 DSM strains: An organic-rich, solid culture medium comprised of soluble starch, peptone, meat extract, yeast extract, potassium phosphate, and agar. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium305.pdf 305. BACILLUS THERMOGLUCOSIDASIUS MEDIUM Soluble starch 10.0 g Peptone 5.0 g Meat extract 3.0 g Yeast extract 3.0 g KH2PO4 3.0 g Agar 30.0 g Distilled water 1000.0 ml Adjust final pH to 7.0. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Bacillus thermoglucosidasius medium DSMZ Medium 303 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium303.pdf 303. SIMONSIELLA MEDIUM Trypticase (BBL) 17.0 g Phytone (BBL) 3.0 g NaCl 5.0 g K2HPO4 2.5 g Yeast extract 4.0 g Horse serum 100.0 ml Agar, if necessary 15.0 g Distilled water 1000.0 ml Prepare the medium without horse serum. After autoclaving cool to 50˚C, then add 100 ml/l of sterile horse serum. Transfer cultures when actively growing. Cultures die rapidly in stationary growth phase! © 2007 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, solid culture medium comprised of trypticase (trypticase peptone), phytone (phytone peptone), sodium chloride, potassium phosphate, yeast xtract, horse serum, and agar. Carrine Blank Simonsiella medium DSMZ Medium 326 An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium chloride, cobalt chloride, trace elements, yeast extract, trypticase peptone, resazurin, sodium bicarbonate, sucrose, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Clostridium stercorarium medium A-GÖ medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium326.pdf 326. CLOSTRIDIUM STERCORARIUM (A-GÖ) MEDIUM KH2PO4 3.30 g MgCl2 x 6 H2O 0.16 g CoCl2 x 6 H2O 12.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 0.50 mg NaHCO3 4.00 g Sucrose 5.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, sucrose, vitamins, cysteine, and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add sucrose, cysteine and sulfide to the medium after autoclaving from sterile stock solutions autoclaved under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are prepared under N2 gas and sterilized by filtration. Adjust pH of completed medium to pH 6.8 – 7.2. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 323 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium323.pdf 323. TREPONEMA SACCHAROPHILUM MEDIUM Salt solution A 200.00 ml Salt solution B 200.00 ml Resazurin 1.00 mg Yeast extract (Oxoid) 2.00 g Trypticase (BBL) 2.00 g n-Butyric acid 0.40 ml iso-Butyric acid 0.40 ml DL-2-Methylbutyric acid 0.20 ml n-Valeric acid 0.20 ml iso-Valeric acid 0.20 ml Cysteine-HCl x H2O 1.00 g NaHCO3 5.00 g Glucose 2.00 g Distilled water 480.00 ml Adjust pH to 6.7 - 7.0. Gas atmosphere: 100% CO2. Dissolve ingredients except NaHCO3 and glucose in 880 ml water (including salt solutions). Adjust pH to 7.0 with KOH. Boil for 5min, then cool under CO2 gas to room temperature and distribute under CO2 gas atmosphere (8.8 ml/anaerobic culture tube). After autoclaving add 1 ml of a filter-sterilized NaHCO3 solution (5% w/v) and 0.2 ml of a filter-sterilized glucose solution (10% w/v) to each tube. Check final pH which should be 6.7 - 7.0. Salt solution A: CaCl2 x 2 H2O 0.59 g MgSO4 x 7 H2O 0.96 g Distilled water 1000.00 ml Salt solution B: KH2PO4 2.25 g K2HPO4 2.25 g NaCl 4.50 g (NH4)2SO4 4.50 g Distilled water 1000.00 ml © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of calcium chloride, magnesium sulfate, potassium phosphate, sodium chloride, ammonium sulfate, resazurin, yeast extract, trypticase (trypticase peptone), butyric acid, isobutyric acid, 2-methylbutyric acid, valeric acid, isovaleric acid, cysteine hydrochloride, sodium bicarbonate, and glucose. Prepared under an atmosphere of carbon dioxide. Treponema saccharophilum medium DSM strains: DSMZ Medium 325 Syntrophomonas bryantii medium An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, trace elements, resazurin, sodium bicarbonate, sodium caproate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium325.pdf 325. SYNTROPHOMONAS BRYANTII MEDIUM Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 5.00 g Na-caproate 1.40 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.40 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, caproate, vitamins and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Dispense under same gas atmosphere in Hungate-type tubes or serum vials and autoclave. After sterilization add caproate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of final medium to 7.2 – 7.4. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 322 DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium322.pdf 322. METHANOSPHAERA (MCB-3) MEDIUM Rumen fluid, clarified (see medium 1310) 100.00 ml Trypticase peptone (BD BBL) 2.00 g Yeast extract 2.00 g Na-acetate 0.50 g Na-formate 0.50 g Trace element solution (see medium 141) 10.00 ml Na2SeO4 1.90 mg NiCl2 x 6 H2O 0.70 mg FeSO4 x 7 H2O 3.00 mg K2HPO4 0.60 g KH2PO4 2.80 g (NH4)2SO4 0.30 g NH4Cl 1.00 g NaCl 0.60 g MgSO4 x 7 H2O 0.15 g CaCl2 x 2 H2O 0.08 g Resazurin 0.50 mg Methanol 5.00 ml Vitamin solution (see medium 141) 20.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 900.00 ml Dissolve ingredients except methanol, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Adjust pH of medium to 6.8 – 7.0, then dispense in anoxic Hungate-type tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add methanol, vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 6.7 - 6.9, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. © 2014 DSMZ GmbH - All rights reserved Carrine Blank Methanosphaera (MCB-3) medium An organic-rich, liquid culture medium comprised of clarified rumen fluid, trypticase peptone, yeast extract, sodium acetate, sodium formate, trace elements, sodium selenate, nickel chloride, ferrous sulfate, potassium phosphate, ammonium sulfate, ammonium chloride, sodium chloride, magnesium sulfate, calcium chloride, resazurin, methanol, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen. Methanosphaera medium MCB-3 medium DSMZ Medium 321 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium321.pdf 321. METHANOCORPUSCULUM AGGREGANS MEDIUM NH4Cl 1.00 g K2HPO4 x 3 H2O 0.40 g MgCl2 x 6 H2O 0.40 g Mineral solution (see below) 50.00 ml Na-formate 5.00 g Na-acetate 1.00 g Trypticase (BD BBL) 1.00 g Yeast extract 1.00 g Trace element solution (see medium 141) 10.00 ml Sludge fluid (see medium 119) or 5.00 ml Rumen fluid, clarified (see medium 1310) 5.00 ml Resazurin 0.50 mg Na2CO3 1.50 g L-Cysteine-HCl x H2O 0.20 g Na2S x 9 H2O 0.20 g Distilled water 935.00 ml Dissolve ingredients except carbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense medium under same gas atmosphere in anoxic Hungate-type tubes and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use check pH of completed medium and adjust to 6.8 – 7.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Mineral solution: KH2PO4 6.00 g (NH4)2SO4 6.00 g NaCl 12.00 g MgSO4 x 7 H2O 2.60 g CaCl2 x 2 H2O 0.16 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, magnesium chloride, mineral solution, sodium formate, sodium acetate, trypticase (trypticase peptone), yeast extract, trace elements, sludge fluid, rumen fluid, resazurin, sodium carbonate, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and carbon dioxide. Methanocorpusculum aggregans medium DSM strains: DSMZ Medium 319 Sporohalobacter lotetii medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium319.pdf 319. SPOROHALOBACTER LORTETII MEDIUM Casamino acids 2.00 g Nutrient broth 2.00 g Yeast extract 2.00 g L-Glutamic acid 4.00 g NaCl 105.00 g KCl 0.75 g Trace element solution (see medium 141) 10.00 ml FeSO4 x 7 H2O 2.00 mg Resazurin 0.50 mg L-Cysteine-HCl x H2O 0.50 g MgCl2 x 6 H2O 10.00 g CaCl2 x 2 H2O 3.70 g Vitamin solution (see medium 141) 10.00 ml Distilled water 1000.00 ml Dissolve ingredients (except cysteine, magnesium chloride, calcium chloride and vitamins), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Thereafter, add and dissolve cysteine, adjust pH to 6.5 and dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add magnesium chloride and calcium chloride from sterile anoxic stock solutions autoclaved under 100% N2 gas and vitamins from a filter-sterilized stock solution prepared under N2 gas. Note: For agar medium add 20.00 g/l agar, 5.00 g/l CaCO3, and 2.00 g/l soluble starch. © 2014 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank An organic-rich, liquid culture medium comprised of casamino acids, nutrient broth, yeast extract, L-glutamic acid, sodium chloride, potassium chloride, trace elements, ferrous sulfate, resazurin, L-cysteine hydrochloride, magnesium chloride, calcium chloride, and vitamins. Prepared under an atmosphere of dinitrogen. DSMZ Medium 318c http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium318c.pdf 318c. PELOBACTER ACIDIGALLICI MEDIUM KH2PO4 0.30 g NaCl 0.60 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.08 g Trace element solution (see medium 144) 10.00 ml NH4Cl 1.00 g Yeast extract 0.50 g Trypticase (BBL) 0.50 g Resazurin 0.50 mg KHCO3 4.50 g Vitamin solution (see medium 141) 10.00 ml Na-gallate 1.90 g L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, gallate, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, gallate, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and gallate are sterilized by filtration. Prior to use adjust pH of completed medium to 7.3. © 2014 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank Pelobacter acidigallici medium An organic-rich, liquid culture medium comprised of potassium phosphate, sodium chloride, magnesium chloride, calcium chloride, trace elements, ammonium chloride, yeast extract, trypticase (trypticase peptone), resazurin, potassium bicarbonate, vitamins, sodium gallate, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 318b Clostridium neopropionicum medium DSM strains: Carrine Blank An organic-rich, liquid culture medium comprised of potassium phosphate, sodium chloride, magnesium chloride, calcium chloride, trace elements, ammonium chloride, yeast extract, trypticase (trypticase peptone), resazurin, potassium bicarbonate, vitamins, ethanol, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium318b.pdf 318b. CLOSTRIDIUM NEOPROPIONICUM MEDIUM KH2PO4 0.30 g NaCl 0.60 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.08 g Trace element solution (see medium 144) 10.00 ml NH4Cl 1.00 g Yeast extract 0.50 g Trypticase (BD BBL) 0.50 g Resazurin 0.50 mg KHCO3 4.00 g Ethanol 1.30 ml Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, ethanol, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then dispense under 80% N2 and 20% CO2 gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ethanol, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins are prepared under N2 gas and sterilized by filtration. Adjust final pH to 7.0 – 7.2, if necessary. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 318a FUF medium An organic-rich, liquid culture medium comprised of HEPES buffer, potassium phosphate, sodium chloride, magnesium chloride, calcium chloride, trace elements, ammonium chloride, resazurin, potassium bicarbonate, vitamins, 2-furoic acid, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Pelobacter medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium318a.pdf 318a. PELOBACTER (FUF) MEDIUM HEPES (SIGMA) 11.92 g KH2PO4 0.30 g NaCl 0.60 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.08 g Trace element solution (see medium 144) 10.00 ml NH4Cl 1.00 g Resazurin 0.50 mg KHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml 2-Furoic acid (SIGMA) 2.24 g L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, furoic acid, cysteine and sulfide and adjust pH of solution to 7.0. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add furoic acid (stock solution neutralized with NaOH), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.0 – 7.2. © 2014 DSMZ GmbH - All rights reserved DSM strains: Pelobacter (FUF) medium Carrine Blank DSMZ Medium 317 MP medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium317.pdf 317. METHANOSARCINA (MP) MEDIUM NH4Cl 1.00 g K2HPO4 0.40 g MgCl2 x 6 H2O 0.20 g Mineral solution (see below) 50.00 ml Trace element solution (see medium 141) 10.00 ml Yeast extract 1.00 g Resazurin 0.50 mg Na2CO3 1.00 g Methanol 5.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.25 g Distilled water 950.00 ml Dissolve ingredients except carbonate, methanol, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under the same gas atmosphere in anoxic Hungate-type tubes or serum vilas and autoclave. Add methanol, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 6.8 - 7.0. Minerals solution: KH2PO4 6.00 g (NH4)2SO4 6.00 g NaCl 12.00 g MgSO4 x 7 H2O 2.60 g CaCl2 x 2 H2O 0.16 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved Methanosarcina (MP) medium Methanosarcina medium An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, magnesium chloride, mineral solution, trace elements, yeast extract, resazurin, sodium carbonate, methanol, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: DSMZ Medium 316 An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, sodium phosphate, magnesium chloride, trypticase (trypticase peptone), yeast extract, trace elements, vitamins, ferrous sulfate, resazurin, and sodium sulfide. Prepared under an atmosphere of dinitrogen. Carrine Blank Moorella themroacetica (TYE-CO) medium Moorella thermoacetica medium TYE-CO medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium316.pdf 316. MOORELLA THERMOACETICA (TYE-CO) MEDIUM NH4Cl 1.0 g KH2PO4 0.3 g Na2HPO4 x 12 H2O 2.8 g MgCl2 x 6 H2O 0.2 g Trypticase (BD BBL) 10.0 g Yeast extract 3.0 g Trace element solution (see medium 141) 10.0 ml Vitamin solution (see medium 141) 5.0 ml FeSO4 x 7 H2O 1.0 mg Resazurin 0.5 mg Na2S x 9 H2O 0.6 g Distilled water 1000.0 ml Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% carbon monoxide gas. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 315 DSM strains: An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, ammonium sulfate, magnesium sulfate, calcium chloride, ferrous sulfate, trace elements, vitamins, cellobiose or cellulose, resazurin, sodium bicarbonate, cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium315.pdf 315. BACTEROIDES CELLULOSOLVENS MEDIUM NH4Cl 0.68 g K2HPO4 0.30 g KH2PO4 0.18 g (NH4)2SO4 0.15 g MgSO4 x 7 H2O 0.12 g CaCl2 x 2 H2O 0.06 g FeSO4 x 7 H2O 0.02 g Trace element solution (see below) 10.00 ml Vitamin solution (see below) 10.00 ml Cellobiose 5.00 g or Cellulose (i.e. MN 300) 5.00 g Resazurin 1.00 mg NaHCO3 2.00 g Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Adjust pH to 7.0. Gas atmosphere: 80% N2 + 20% CO2. Filter sterilize cellobiose separately. Trace element solution: Nitrilotriacetic acid 1.500 g MgSO4 x 7 H2O 3.000 g MnSO4 x H2O 0.500 g NaCl 1.000 g FeSO4 x 7 H2O 0.100 g CoSO4 x 7 H2O 0.180 g CaCl2 x 2 H2O 0.100 g ZnSO4 x 7 H2O 0.180 g CuSO4 x 5 H2O 0.010 g KAl(SO4)2 x 12 H2O 0.020 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.010 g NiCl2 x 6 H2O 0.025 g Na2SeO3 x 5 H2O 0.300 mg Distilled water 1000.000 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). © 2009 DSMZ GmbH - All rights reserved Carrine Blank Bacteroides cellulosolvens medium DSMZ Medium 314 Ilyobacter polytropus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium314.pdf 314. ILYOBACTER POLYTROPUS MEDIUM NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.30 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.27 g KH2PO4 0.20 g Na2SO4 2.84 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Na-3-hydroxybutyrate 1.40 g Yeast extract 1.00 g Resazurin 0.50 mg NaHCO3 4.50 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.40 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic Hungate-type tubes under 80% N2 and 20% CO2 gas atmosphere and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Prior to use check pH of completed medium and adjust to 7.2 - 7.4, if necessary. © 2014 DSMZ GmbH - All rights reserved Carrine Blank An organic-rich, liquid culture medium comprised of sodium chloride, magnesium chloride, potassium chloride, calcium chloride, ammonium chloride, potassium phosphate, sodium sulfate, trace elements, selenite-tungstate, sodium 3-hydroxybutyrate, yeast extract, resazurin, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: DSMZ Medium 312 Carrine Blank DSM strains: An organic-rich, liquid culture medium comprised of sucrose, casitone, yeast extract, trypticase soy broth without dextrose, thiamine hydrochloride, and vitamin B12 (cobalamin). SCY medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium312.pdf 312. SCY - MEDIUM Sucrose 1.00 g Casitone 0.75 g Yeast extract 0.25 g Trypticase soy broth without dextrose (BBL) 0.17 g Thiamine-HCl x 2 H2O 0.40 mg Vitamin B12 0.01 mg Distilled water 1000.00 ml Adjust pH to 7.1. Sterilize vitamins separately by filtration. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 311b http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311b.pdf 311b. SPOROMUSA MEDIUM (MARINE) NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 25.00 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Betaine x H2O 6.70 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.5 – 7.7. © 2014 DSMZ GmbH - All rights reserved Sporomusa medium (marine) Sporomusa marine medium DSM strains: An organic-rich, liquid culture medium comprised of ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, selenite-tungstate, yeast extract, casitone, betaine, resazurin, potassium phosphate, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank DSMZ Medium 311a Carrine Blank Sporomusa medium (H2/CO2) http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311a.pdf 311a. SPOROMUSA MEDIUM (H2/CO2) NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml DL-Dithiothreitol 0.15 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins and dithiothreitol) and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins and dithiothreitol should be prepared under N2 gas and sterilized by filtration. Adjust pH of completed medium to pH 7.0. For DSM 11379, DSM 11380, DSM 11381 and DSM 11382 supplement medium with 2.50 g/l Na-pyruvate. © 2014 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, selenite-tungstate, yeast extract, casitone, resazurin, potassium phosphate, sodium bicarbonate, vitamins, and dithiothreitol (1,4-dithiothreitol). Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen. Sporomusa medium DSM strains: DSMZ Medium 311a.1 Similar to DSMZ Medium 311a, except sodium pyruvate is added. Carrine Blank DSM 11379 is Sporomusa (not in StrainInfo, no sequences in TaxBrowser) DSM 11380 is Sporomusa (not in StrainInfo, no sequences in TaxBrowser) DSM 11381 is Sporomusa (not in StrainInfo, no sequences in TaxBrowser) DSM 11382 is Acetonema sp. DSM 11382 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311a.pdf 311a. SPOROMUSA MEDIUM (H2/CO2) NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml DL-Dithiothreitol 0.15 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins and dithiothreitol) and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins and dithiothreitol should be prepared under N2 gas and sterilized by filtration. Adjust pH of completed medium to pH 7.0. For DSM 11379, DSM 11380, DSM 11381 and DSM 11382 supplement medium with 2.50 g/l Na-pyruvate. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 311a.1 -< for DSM 11379, DSM 11380, DSM 11381 and DSM 11382 DSMZ Medium 310 DSM strains: An organic-rich, solid culture medium comprised of V-8 juice, calcium carbonate, and agar. V-8 juice agar Salinity derived from the V-8 juice is 1.4g/L NaCl, or 0.14% NaCl). http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium310.pdf 310. V-8 JUICE AGAR *V-8 Juice 200.0 ml CaCO3 3.0 g Agar 15.0 g Fill up with distilled water to 1000.0 ml. Adjust pH to 7.2. * May be replaced by tomato mash if not available. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 336 An organic-rich, liquid culture medium comprised of mineral solution, trace elements, yeast extract, sodium vanillate, vitamins, clarified rumen fluid, resazurin, sodium bicarbonate, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: Oxobacter pfennigii medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium336.pdf 336. OXOBACTER PFENNIGII MEDIUM Solution A: Mineral solution (see medium 335) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 2.00 g Na-vanillate 2.00 g Vitamin solution (see medium 503) 1.00 ml Rumen fluid, clarified (see medium 1310)) 300.00 ml Resazurin 0.50 mg Distilled water 540.00 ml Adjust pH to 6.9 before autoclaving. Solution B: NaHCO3 5.00 g Distilled water 100.00 ml Solution C: L-Cysteine-HCl x H2O 0.24 g Distilled water 10.00 ml Solution D: Na2S x 9 H2O 78.00 mg Distilled water 1.00 ml Prepare solution A under 80% N2 and 20% CO2 gas mixture, distribute under same gas atmosphere 8.9 ml medium per Hungate-type tube and autoclave. Filter sterilize solution B and sparge for 20 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Autoclave solutions C and D under 100% N2 gas. Complete medium by adding the solutions B, C, and D with a syringe to solution A. Adjust final pH of medium to 7.0 - 7.2. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 337 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium337.pdf 337. SYNTROPHOCOCCUS SUCROMUTANS MEDIUM Solution A: Mineral solution (see medium 335) 50.00 ml Trace element solution (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 300.00 ml Trypticase (BBL) or Casitone peptone 5.00 g Na-formate 0.60 g Resazurin 0.50 mg Distilled water 560.00 ml Solution B: NaHCO3 2.50 g Distilled water 50.00 ml Solution C: Vitamin solution (see medium 212) 5.00 ml Solution D: Lactose 5.00 g Distilled water 25.00 ml Solution E: L-Cysteine-HCl x H2O 0.24 g Distilled water 10.00 ml Solution F: Na2S x 9 H2O 78.00 mg Distilled water 1.00 ml Dissolve ingredients of solution A and sparge under 80% N2 and 20% CO2 gas atmosphere for 30 - 45 min to make it anoxic. Thereafter, adjust pH to 6.4 and dispense under same gas atmosphere in culture vessels and autoclave. Anoxic stock solutions C, D, E and F are prepared separately under 100% N2 gas. Solution B is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize solution C. Solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of the completed medium to 6.4 - 6.8. Note: Rumen fluid may be replaced by supplementing the medium with 200 μg/ml of crude egg yolk phosphatidylcholine (Sigma, type IX-E). © 2015 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of mineral solution, trace elements, clarified rumen fluid, trypticase peptone or casitone, sodium formate, resazurin, sodium bicarbonate, vitamins, lactose, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Syntrophococcus sucromutans medium Carrine Blank DSM strains: DSMZ Medium 338 DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium338.pdf 338. CAMPYLOBACTER RECTUS MEDIUM Beef extract (Difco) 3.00 g Trypticase (BBL) 9.00 g Yeast extract 11.00 g NaCl 2.00 g Na2HPO4 0.40 g Na2CO3 0.25 g Haemin 5.00 mg Na-formate 2.00 g Na-fumarate 3.00 g Resazurin 1.00 mg Distilled water 1000.00 ml Adjust pH to 7.5. Prepare the medium anaerobically under 100% N2. Autoclave solutions of haemin (dissolved in 0.1 ml 1N NaOH, then diluted with water to 10 ml and neutralized), formate, and fumarate separately under nitrogen. © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of beef extract, trypticase peptone, yeast extract, sodium chloride, sodium phosphate, sodium carbonate, hemin, sodium formate, sodium fumarate, and resazurin. Prepared under an atmosphere of dinitrogen. Campylobacter rectus medium DSMZ Medium 342 Methanobacterium alcaliphilum medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium342.pdf 342. METHANOBACTERIUM ALCALIPHILUM MEDIUM Solution A: NH4Cl 1.0 g MgCl2 x 6 H2O 0.1 g CaCl2 x 2 H2O 0.1 g K2HPO4 0.4 g Na2SeO3 x 5 H2O 0.2 mg Trace element solution (see medium 141) 10.0 ml Yeast extract 2.0 g Trypticase (BD BBL) 2.0 g Resazurin 0.5 mg L-Cysteine-HCl x H2O 0.5 g Distilled water 780.0 ml Solution B: NaHCO3 10.0 g Na2CO3 0.5 g Distilled water 210.0 ml Solution C: TRIS-HCl buffer 2 M (pH 8.4) 10.0 ml Bring solution A (except cysteine) to the boil, then cool to room temperature under 100% H2 gas. Add cysteine and adjust pH to 7.0 if necessary. Dispense under 100% H2 gas atmosphere in anoxic Hungate-type tubes or serum vials vials (e.g., 3.9 ml per Hungate tube), then autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas atmosphere. Complete the medium by adding appropriate amounts of solutions B and C to the sterile solution A. Adjust pH of completed medium to 8.3 - 8.4, if necessary. After inoculation add sterile H2 gas to 1 - 2 bar overpressure. © 2014 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, liquid culture medium comprised of ammonium chloride, magnesium chloride, calcium chloride, potassium phosphate, sodium selenite, trace elements, yeast extract, trypticase peptone, resazurin, L-cysteine hydrochloride, sodium bicarbonate, sodium carboante, and tris buffer. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen. DSMZ Medium 340 DSM strains: Carrine Blank Capnocytophaga medium An organic-rich, liquid culture medium comprised of trypticase peptone, yeast extract, glucose, sodium chloride, potassium nitrate, and hemin. Prepared under an atmosphere of carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium340.pdf 340. CAPNOCYTOPHAGA MEDIUM Trypticase (BBL) 17.0 g Yeast extract 3.0 g Glucose 3.0 g NaCl 3.0 g KNO3 3.0 g Haemin 3.0 mg Distilled water 1000.0 ml Adjust pH to 7.0. Sterilize glucose separately. Incubate in oxygen-free, 5 - 10% CO2 containing atmosphere. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 343a An organic-rich, liquid culture medium comprised of soluble starch, potassium phosphate, trace elements, nickel chloride, sodium chloride, artificial seawater, yeast extract, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen. Thermosipho melanesiensis medium DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium343a.pdf 343a. THERMOSIPHO MELANESIENSIS MEDIUM Starch, soluble 5.0 g KH2PO4 0.5 g Trace element solution (see medium 141) 15.0 ml NiCl2 x 6 H2O 2.0 mg NaCl 20.0 g Artificial sea water (see medium 343) 250.0 ml Yeast extract (Difco) 2.0 g Resazurin 0.5 mg L-Cysteine-HCl x H2O 0.5 g Na2S x 9 H2O 0.5 g Distilled water 750.0 ml Dissolve ingredients (except sulfide and cysteine) and adjust pH to 6.5. Boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere, dispense under same gas atmosphere in culture vessels (30% of volume) and autoclave. Add sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Check pH of completed medium and adjust to 6.5, if necessary. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 343 Thermotoga neapolitana medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium343.pdf 343. THERMOTOGA NEAPOLITANA MEDIUM Starch, soluble 5.0 g KH2PO4 0.5 g Trace element solution (see medium 141) 15.0 ml NiCl2 x 6 H2O 2.0 mg Artificial sea water (see below) 250.0 ml Yeast extract (Difco) 2.0 g Resazurin 0.5 mg L-Cysteine-HCl x H2O 0.5 g Na2S x 9 H2O 0.5 g Distilled water 750.0 ml Dissolve ingredients (except sulfide and cysteine) and adjust pH to 6.5. Boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere, dispense under same gas atmosphere in culture vessels (30% of volume) and autoclave. Add sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Check pH of completed medium and adjust to 6.5, if necessary. Artificial sea water: NaCl 27.70 g MgSO4 x 7 H2O 7.00 g MgCl2 x 6 H2O 5.50 g KCl 0.65 g NaBr 0.10 g H3BO3 30.00 mg SrCl2 x 6 H2O 15.00 mg Citric acid 10.00 mg KI 0.05 mg CaCl2 x 2 H2O 2.25 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved Carrine Blank An organic-rich, liquid culture medium comprised of soluble starch, potassium phosphate, trace elements, nickel chloride, artificial seawater, yeast extract, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen. DSM strains: DSMZ Medium 344 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium344.pdf 344. FLEXIBACTER MEDIUM Na-glutamate 5.0 g MgSO4 x 7 H2O 1.0 g Yeast extract (Difco) 1.0 g Glucose (sterilized separately) 2.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved Flexibacter medium DSM strains: Carrine Blank An organic rich, solid culture medium comprised of monosodium glutamate, magnesium sulfate, yeast extract, glucose, and agar. DSMZ Medium 347 DSM strains: Syntrophomonas sapovorans medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium347.pdf 347. SYNTROPHOMONAS SAPOVORANS MEDIUM Solution A: Mineral solution (see medium 212) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BD BBL) 1.00 g PIPES (SIGMA) 15.00 g Resazurin 0.50 mg Distilled water 780.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: Na-laurate 2.78 g Distilled water 25.00 ml Solution E: CaCl2 x 2 H2O 1.84 g Distilled water 10.00 ml Solution F: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution G: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.0, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in anoxic culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with 80% N2 and 20% CO2 gas mixture for at least 15 min. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D, E, F and G are autoclaved under 100% N2 gas. To complete the medium, appropriate amounts of the solutions B to G are added to solution A in the sequence indicated. Adjust final pH of medium to 7.2. © 2014 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of mineral solution, trace elements, clarified rumen fluid, trypticase peptone, PIPES, resazurin, sodium bicarbonate, vitamins, sodium laurate, calcium chloride, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 346 Carrine Blank DSM strains: Desulfotomaculum sapomandens medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium346.pdf 346. DESULFOTOMACULUM SAPOMANDENS MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g CaCl2 x 2 H2O 0.15 g MgCl2 x 6 H2O 0.40 g NaCl 1.00 g Trace elements SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg Distilled water 965.00 ml Solution B: NaHCO3 1.00 g Distilled water 20.00 ml Solution C: Ethanol 1.00 ml Distilled water 10.00 ml Solution D: Na-benzoate, 7% w/v 0.70 g Distilled water 10.00 ml Solution E: Rumen fluid, clarified (see medium 1310) 1.00 ml Solution F: Vitamin solution (see medium 141) 10.00 ml Solution G: Na-dithionite 25.00 mg Distilled water 1.00 ml Solution H: Na2S x 9 H2O 50.00 mg Distilled water 1.70 ml Dissolve ingredients of solution A and sparge under 100% N2 gas atmosphere for 30 - 45 min to make it anoxic. Thereafter, dispense solution A under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions C, D, E, F, G and H are prepared separately under 100% N2 gas atmosphere. Filter sterilize solutions D, F and G. Solutions B to H are added to the sterile solution A in the sequence as indicated. Adjust the pH of the completed medium to 7.2 - 7.5. © 2015 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, calcium chloride, magnesium chloride, sodium chloride, trace elements, resazurin, sodium bicarbonate, ethanol, sodium benzoate, clarified rumen fluid, vitamins, sodium dithionite, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 347a Syntrophomonas zehnderi medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium347a.pdf 347a. SYNTROPHOMONAS ZEHNDERI MEDIUM Solution A: Mineral solution (see medium 212) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Na2SO4 2.80 g PIPES (SIGMA) 15.00 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: Na-laurate 2.78 g Distilled water 25.00 ml Solution E: CaCl2 x 2 H2O 1.84 g Distilled water 10.00 ml Solution F: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution G: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.0, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with 80% N2 and 20% CO2 gas mixture for at least 15 min. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D, E, F and G are autoclaved under 100% N2 gas. To complete the medium, appropriate amounts of the solutions B to G are added to solution A in the sequence indicated. Adjust final pH of medium to 7.2. © 2014 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, liquid culture medium comprised of mineral solution, trace elements, sodium sulfate, PIPES, resazurin, sodium bicarbonate, vitamins, sodium laurate, calcium chloride, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 348 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium348.pdf 348. METHANOHALOPHILUS PORTUCALENSIS MEDIUM NaCl 120.00 g MgCl2 x 6 H2O 7.00 g MgSO4 x 7 H2O 6.00 g CaCl2 x 2 H2O 0.50 g K2HPO4 x 3 H2O 0.40 g NH4Cl 1.00 g KCl 3.80 g Trace elements SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Trypticase (BBL) 2.00 g Resazurin 0.50 mg L-Cysteine-HCl x H2O 0.50 g Na2CO3 1.50 g Trimethylamine-HCl 1.90 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Dissolve ingredients except cysteine, carbonate, trimethylamine and sulfide, bring medium to the boil, then cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Add cysteine and adjust pH to 6.5, then distribute into anoxic Hungatetype tubes or serum vials under the same gas atmosphere and autoclave. Thereafter, add trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Control final pH and adjust to pH 7.3 - 7.4, if necessary. © 2015 DSMZ GmbH - All rights reserved An organic-rich, liquid culture comprised of sodium chloride, magnesium chloride, magnesium sulfate, calcium chloride, potassium phosphate, ammonium chloride, potassium chloride, trace elements, selenite-tungstate, yeast extract, trypticase peptone, resazurin, L-cysteine hydrochloride, sodium carbonate, trimethylamine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Methanohalophilus portucalensis medium DSM strains: DSMZ Medium 350 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium350.pdf 350. CELLULOMONAS FERMENTANS MEDIUM K2HPO4 2.21 g KH2PO4 1.50 g (NH4)2SO4 1.30 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.02 g FeSO4 x 7 H2O 1.25 mg Yeast extract 5.00 g Cellobiose 5.00 g or Cellulose (MN 300) 5.00 g NaHCO3 0.80 g Resazurin 1.00 mg Cysteine-HCl x H2O 0.50 g Distilled water 1000.00 ml Adjust pH to 7.4 with 8 M NaOH. Prepare the medium anaerobically under 100% nitrogen. Cellobiose, NaHCO3, and cysteine are sterilized separately by autoclaving under N2. For aerobic growth omit resazurin and cysteine. © 2007 DSMZ GmbH - All rights reserved Carrine Blank An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium sulfate, magnesium chloride, calcium chloride, ferrous sulfate, yeast extract, cellobiose or cellulose, sodium bicarbonate, resazurin, and cysteine hydrochloride. Prepared under an atmosphere of dinitrogen. DSM strains: Cellulomonas fermentans medium vitamin solution VA for DSMZ Medium 351 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium351.pdf 351. RHODOBACTER ADRIATICUS MEDIUM Na acetate 0.82 g Yeast extract 0.1 g Na2S x 9H2O 0.06 d K2HPO4 1.0 g CaCl2 x 2 H2O 0.1 g MgCl2 x 6 H2O 0.5 g NH4Cl 1.0 g NaHCO3 3.0 g NaCl 25.0 g Na-ascorbate 0.5 g Trace element solution (SLA) 1.0 ml Distilled water 1000.0 ml Adjust pH to 7.0. The medium may be prepared without the addition of Na acetate, yeast extract and Na2S x 9H2O and may be added to the sterile medium from sterile stock solutions. Prepare the medium under an atmosphere of N2. Dispense into screw capped or crimp top bottles fitted with a rubber septum. Sterilize at 121˚C for 15 minutes in screw-caped bottles. After sterilization add 1.0 ml/l filter-sterilized vitamin solution (VA, see below). Incubate at 30˚C at a light intensity of 1500 lux under anaerobic conditions. Trace element solution (SLA): FeCl2 x 4 H2O 1.8 g CoCl2 x 6 H2O 250.0 mg NiCl2 x 6 H2O 10.0 mg CuCl2 x 2 H2O 10.0 mg MnCl2 x 4 H2O 70.0 mg ZnCl2 100.0 mg H3BO3 500.0 mg Na2MoO4 x 2 H2O 30.0 mg Na2SeO3 x 5 H2O 10.0 mg Distilled water 1000.0 ml Adjust pH to 2.0 - 3.0. Vitamin solution (VA): Biotin 10.0 mg Nicotinic acid amide 35.0 mg Thiamine-HCl x 2 H2O 30.0 mg p-Aminobenzoic acid 20.0 mg Pyridoxal hydrochloride 10.0 mg Ca-pantothenate 10.0 mg Vitamin B12 5.0 mg Distilled water 100.0 ml © 2007 DSMZ GmbH - All rights reserved A vitamin solution comprised of biotin, nicotinic acid amide (nicotinamide), thiamine hydrochloride, p-aminobenzoic acid (4-aminobenzoic acid), pyridoxal hydrochloride, calcium pantothenate, and vitamin B12 (cobalamin). trace elements solution SLA for DSMZ Medium 351 A trace elements solution comprised of ferrous chloride, cobalt chloride, nickel chloride, copper chloride, manganese chloride, zinc chloride, boric acid, sodium molybdate, and sodium selenite. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium351.pdf 351. RHODOBACTER ADRIATICUS MEDIUM Na acetate 0.82 g Yeast extract 0.1 g Na2S x 9H2O 0.06 d K2HPO4 1.0 g CaCl2 x 2 H2O 0.1 g MgCl2 x 6 H2O 0.5 g NH4Cl 1.0 g NaHCO3 3.0 g NaCl 25.0 g Na-ascorbate 0.5 g Trace element solution (SLA) 1.0 ml Distilled water 1000.0 ml Adjust pH to 7.0. The medium may be prepared without the addition of Na acetate, yeast extract and Na2S x 9H2O and may be added to the sterile medium from sterile stock solutions. Prepare the medium under an atmosphere of N2. Dispense into screw capped or crimp top bottles fitted with a rubber septum. Sterilize at 121˚C for 15 minutes in screw-caped bottles. After sterilization add 1.0 ml/l filter-sterilized vitamin solution (VA, see below). Incubate at 30˚C at a light intensity of 1500 lux under anaerobic conditions. Trace element solution (SLA): FeCl2 x 4 H2O 1.8 g CoCl2 x 6 H2O 250.0 mg NiCl2 x 6 H2O 10.0 mg CuCl2 x 2 H2O 10.0 mg MnCl2 x 4 H2O 70.0 mg ZnCl2 100.0 mg H3BO3 500.0 mg Na2MoO4 x 2 H2O 30.0 mg Na2SeO3 x 5 H2O 10.0 mg Distilled water 1000.0 ml Adjust pH to 2.0 - 3.0. Vitamin solution (VA): Biotin 10.0 mg Nicotinic acid amide 35.0 mg Thiamine-HCl x 2 H2O 30.0 mg p-Aminobenzoic acid 20.0 mg Pyridoxal hydrochloride 10.0 mg Ca-pantothenate 10.0 mg Vitamin B12 5.0 mg Distilled water 100.0 ml © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 351 Carrine Blank DSM strains: Rhodobacter adriaticus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium351.pdf 351. RHODOBACTER ADRIATICUS MEDIUM Na acetate 0.82 g Yeast extract 0.1 g Na2S x 9H2O 0.06 d K2HPO4 1.0 g CaCl2 x 2 H2O 0.1 g MgCl2 x 6 H2O 0.5 g NH4Cl 1.0 g NaHCO3 3.0 g NaCl 25.0 g Na-ascorbate 0.5 g Trace element solution (SLA) 1.0 ml Distilled water 1000.0 ml Adjust pH to 7.0. The medium may be prepared without the addition of Na acetate, yeast extract and Na2S x 9H2O and may be added to the sterile medium from sterile stock solutions. Prepare the medium under an atmosphere of N2. Dispense into screw capped or crimp top bottles fitted with a rubber septum. Sterilize at 121˚C for 15 minutes in screw-caped bottles. After sterilization add 1.0 ml/l filter-sterilized vitamin solution (VA, see below). Incubate at 30˚C at a light intensity of 1500 lux under anaerobic conditions. Trace element solution (SLA): FeCl2 x 4 H2O 1.8 g CoCl2 x 6 H2O 250.0 mg NiCl2 x 6 H2O 10.0 mg CuCl2 x 2 H2O 10.0 mg MnCl2 x 4 H2O 70.0 mg ZnCl2 100.0 mg H3BO3 500.0 mg Na2MoO4 x 2 H2O 30.0 mg Na2SeO3 x 5 H2O 10.0 mg Distilled water 1000.0 ml Adjust pH to 2.0 - 3.0. Vitamin solution (VA): Biotin 10.0 mg Nicotinic acid amide 35.0 mg Thiamine-HCl x 2 H2O 30.0 mg p-Aminobenzoic acid 20.0 mg Pyridoxal hydrochloride 10.0 mg Ca-pantothenate 10.0 mg Vitamin B12 5.0 mg Distilled water 100.0 ml © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of sodium acetate, yeast extract, sodium sulfide, potassium phosphate, calcium chloride, magnesium chloride, ammonium chloride, sodium bicarbonate, sodium chloride, sodium ascorbate, trace elements and vitamins. Prepared under an atmosphere of dinitrogen. DSMZ Medium 352 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium352.pdf 352. AZOSPIRILLUM AMAZONENSE MEDIUM K2HPO4 0.200 g KH2PO4 0.600 g CaCl2 x 2 H2O 0.020 g MgSO4 x 7 H2O 0.200 g Na2MoO4 x 2 H2O 0.002 g FeCl3 0.010 g Bromothymol blue (0.5% in 0.2N KOH) 5.000 ml Sucrose 5.000 g Agar 1.750 g Distilled water 1000.000 ml Adjust pH to 6.0. © 2007 DSMZ GmbH - All rights reserved DSM strains: Azospirillum amazonense medium An organic-rich, solid culture medium comprised of potassium phosphate, calcium chloride, magnesium sulfate, sodium molybdate, ferric trichloride, bromothymol blue, sucrose, and agar. Carrine Blank DSMZ Medium 357 Carrine Blank xxxx http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium357.pdf 357. FLEXIBACTER CANADENSIS MEDIUM MgSO4 x 7 H2O 0.1 g KNO3 0.1 g CaCl2 x 2 H2O 0.1 g Na-glycerophosphate 0.1 g Trace elements SL-10 (see medium 320) 1.0 ml Tris 1.0 g Thiamine-HCl x 2 H2O 1.0 mg Casamino acids 1.0 g Glucose (sterilized separately) 1.0 g Vitamin B12 1.0 μg Agar 10.0 g Distilled water 1000.0 ml Adjust pH to 7.5. © 2007 DSMZ GmbH - All rights reserved DSM strains: Flexibacter canadensis medium DSMZ Medium 354 An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium sulfate, magnesium citrate, L-asparagine, potato flour, malachite green, glycerol, and homogenized chicken eggs. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium354.pdf 354. LÖWENSTEIN-JENSEN MEDIUM KH2PO4 2.50 g MgSO4 0.24 g Mg-citrate 0.60 g L-Asparagin 3.60 g Potato flour 30.00 g Malachite green 0.40 g Distilled water 600.00 ml Addition: Glycerol 12.00 ml *Fresh egg mixture (yolk and whites) 1000.00 ml pH 7.0 Sterilize medium for 30 min at 121˚C, cool to 30˚C and add fresh egg mixture carefully to the medium. * You need for 1 l of egg mixture 20 - 25 fresh eggs (not older then 1 week). Clean the eggs, wipe away with 70% alcohol, open the eggs and homogenize the egg mixture carefully. © 2007 DSMZ GmbH - All rights reserved Carrine Blank LÖwenstein-Jensen medium DSM strains: DSMZ Medium 358 Carrine Blank Acidianus medium (aerobic) http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium358.pdf 358. ACIDIANUS MEDIUM (AEROBIC) (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Sulfur, powder 5.00 g Yeast extract (Difco) 1.00 g Distilled water 1000.00 ml Dissolve ingredients, bring medium to the boil, then cool to room temperature and adjust pH to 2.5 using 10 N H2SO4. Dispense under air atmosphere enriched with 1 - 10% CO2 in suitable culture vessels (e.g., 20 ml medium in 100 ml serum bottles) while keeping the sulfur suspended by magnetic stirring. For sterilization sealed bottles with medium are heated in a boiling water bath for 2 - 3 h on each of 3 successive days. Note: Inoculate with 5% (w/v) culture. Incubate without shaking. © 2015 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric trichloride, manganese chloride, sodium tetraborate, zinc sulfate, copper chloride, sodium molybdate, vanadyl sulfate, cobalt sulfate, elemental sulfur, and yeast extract. Prepared under an atmosphere of carbon dioxide. Acidianus aerobic medium DSMZ Medium 358a http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium358a.pdf 358a. ACIDIANUS MEDIUM (ANAEROBIC) (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Sulfur, powder 5.00 g Yeast extract (Difco) 0.50 g Distilled water 1000.00 ml Dissolve ingredients, bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture and adjust pH to 2.5 using 10 N H2SO4. Dispense under same gas mixture in suitable culture vessels (e.g., 20 ml medium in 100 ml serum bottles) while keeping the sulfur suspended by magnetic stirring. For sterilization sealed bottles with medium are heated in a boiling water bath for 2 - 3 h on each of 3 successive days. Pressurize inoculated bottles to 1 bar overpressure with 80% H2 and 20% CO2 gas mixture. Note: Inoculate with 5% (w/v) culture. Incubate without shaking. For DSM 3772 reduce amount of yeast extract to 0.02 g/l. For DSM 6296 reduce amount of yeast extract to 0.20 g/l and adjust pH to 2.5 – 3.0. Pressurize inoculated bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. For DSM 6334 reduce amount of yeast extract to 0.20 g/l. Pressurize inoculated bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved Acidianus anaerobic medium Carrine Blank An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric trichloride, manganese chloride, sodium tetraborate, zinc sulfate, copper chloride, sodium molybdate, vanadyl sulfate, cobalt sulfate, elemental sulture, and yeast extract. Prepared under an atmosphere of dihydrogen and carbon dioxide. Acidianus medium (anaerobic) DSM strains: DSMZ Medium 358a.3 Carrine Blank DSMZ Medium 358a.3 -< for DSM 6334 Similar to DSMZ Medium 358a, except the concentration of yeast extract is decreased. The overpressure of dihydrogen and carbon dioxide are increased. DSM 6334 is Acidianus brierleyi http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium358a.pdf 358a. ACIDIANUS MEDIUM (ANAEROBIC) (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Sulfur, powder 5.00 g Yeast extract (Difco) 0.50 g Distilled water 1000.00 ml Dissolve ingredients, bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture and adjust pH to 2.5 using 10 N H2SO4. Dispense under same gas mixture in suitable culture vessels (e.g., 20 ml medium in 100 ml serum bottles) while keeping the sulfur suspended by magnetic stirring. For sterilization sealed bottles with medium are heated in a boiling water bath for 2 - 3 h on each of 3 successive days. Pressurize inoculated bottles to 1 bar overpressure with 80% H2 and 20% CO2 gas mixture. Note: Inoculate with 5% (w/v) culture. Incubate without shaking. For DSM 3772 reduce amount of yeast extract to 0.02 g/l. For DSM 6296 reduce amount of yeast extract to 0.20 g/l and adjust pH to 2.5 – 3.0. Pressurize inoculated bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. For DSM 6334 reduce amount of yeast extract to 0.20 g/l. Pressurize inoculated bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 358a.2 DSMZ Medium 358a.2 -< for DSM 6296 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium358a.pdf 358a. ACIDIANUS MEDIUM (ANAEROBIC) (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Sulfur, powder 5.00 g Yeast extract (Difco) 0.50 g Distilled water 1000.00 ml Dissolve ingredients, bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture and adjust pH to 2.5 using 10 N H2SO4. Dispense under same gas mixture in suitable culture vessels (e.g., 20 ml medium in 100 ml serum bottles) while keeping the sulfur suspended by magnetic stirring. For sterilization sealed bottles with medium are heated in a boiling water bath for 2 - 3 h on each of 3 successive days. Pressurize inoculated bottles to 1 bar overpressure with 80% H2 and 20% CO2 gas mixture. Note: Inoculate with 5% (w/v) culture. Incubate without shaking. For DSM 3772 reduce amount of yeast extract to 0.02 g/l. For DSM 6296 reduce amount of yeast extract to 0.20 g/l and adjust pH to 2.5 – 3.0. Pressurize inoculated bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. For DSM 6334 reduce amount of yeast extract to 0.20 g/l. Pressurize inoculated bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 358a, except the concentration of yeast extract is decreased and the pH is increased to 2.5-3.0. The overpressure of dihydrogen and carbon dioxide is increased. Carrine Blank DSM 6296 is Stygiolobus azoricus DSM 6296 DSMZ Medium 358a.1 DSMZ Medium 358a.1 -< for DSM 3772 Similar to DSMZ Medium 358a, except the concentration of yeast extract is decreased. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium358a.pdf 358a. ACIDIANUS MEDIUM (ANAEROBIC) (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Sulfur, powder 5.00 g Yeast extract (Difco) 0.50 g Distilled water 1000.00 ml Dissolve ingredients, bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture and adjust pH to 2.5 using 10 N H2SO4. Dispense under same gas mixture in suitable culture vessels (e.g., 20 ml medium in 100 ml serum bottles) while keeping the sulfur suspended by magnetic stirring. For sterilization sealed bottles with medium are heated in a boiling water bath for 2 - 3 h on each of 3 successive days. Pressurize inoculated bottles to 1 bar overpressure with 80% H2 and 20% CO2 gas mixture. Note: Inoculate with 5% (w/v) culture. Incubate without shaking. For DSM 3772 reduce amount of yeast extract to 0.02 g/l. For DSM 6296 reduce amount of yeast extract to 0.20 g/l and adjust pH to 2.5 – 3.0. Pressurize inoculated bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. For DSM 6334 reduce amount of yeast extract to 0.20 g/l. Pressurize inoculated bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved DSM 3772 is Acidianus ambivalens Carrine Blank DSMZ Medium 359 DSM strains: An organic-rich, solid culture medium comprised of glucose, fructose, yeast extract, calcium carbonate, vitamin B12 (cobalamin), and agar. GFY medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium359.pdf 359. GFY MEDIUM Glucose 5.000 g Fructose 5.000 g Yeast extract 5.000 g CaCO3 3.000 g Vitamin B12 0.002 g Agar 15.000 g Distilled water 1000.000 ml © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 360 YPM medium Carrine Blank An organic-rich, solid culture medium comprised of yeast extract, peptone, mannitol, and agar. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium360.pdf 360. YPM MEDIUM Yeast extract 5.0 g Peptone 3.0 g Mannitol 25.0 g Agar 12.0 g Distilled water 1000.0 ml pH not adjusted. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 362 Carrine Blank Flavobacterium tirrenicum medium DSM strains: Similar to DSMZ Medium 1, except ethanolamine and sodium chloride are added, and the pH is increased to 7.5. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium362.pdf 362. FLAVOBACTERIUM TIRRENICUM MEDIUM To medium 1 add 2 g/l of ethanolamine and 10 g/l NaCl and adjust pH to 7.5. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 364 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium364.pdf 364. PYEA MEDIUM Peptone 10.0 g Yeast extract 10.0 g NaCl 5.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: An organic-rich, solid culture medium comprised of peptone, yeast extract, sodium chloride, and agar. PYEA medium DSMZ Medium 363.1 Similar to DSMZ Medium 363, except antipyrin is omitted and L-phenylalanine is added. DSMZ Medium 363.1 -< for DSM 2118 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium363.pdf 363. PHENYLOBACTERIUM MEDIUM Antipyrine 1.00 g KH2PO4 0.30 g Na2HPO4 x 12 H2O 0.70 g (NH4)2HPO4 0.70 g NH4H2PO4 0.30 g (NH4)2SO4 0.10 g CaCl2 x 6 H2O 0.05 g MgSO4 x 7 H2O 0.25 g H3BO3 0.50 mg CuSO4 x 5 H2O 0.04 mg KI 0.10 mg FeCl3 x 6 H2O 0.20 mg MnSO4 x 4 H2O 0.40 mg ZnSO4 x 7 H2O 0.40 mg (NH4)2MoO4 0.20 mg Biotin 0.10 mg Vitamin B12 0.03 mg Distilled water 1000.00 ml For DSM 2118, replace antipyrin by 1 g/l L-phenylalanine. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSM 2118 is not in www.dsmz.de or in TaxBrowser. StrainInof lists this as Phenylobacterium immobile. DSMZ Medium 363 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium363.pdf 363. PHENYLOBACTERIUM MEDIUM Antipyrine 1.00 g KH2PO4 0.30 g Na2HPO4 x 12 H2O 0.70 g (NH4)2HPO4 0.70 g NH4H2PO4 0.30 g (NH4)2SO4 0.10 g CaCl2 x 6 H2O 0.05 g MgSO4 x 7 H2O 0.25 g H3BO3 0.50 mg CuSO4 x 5 H2O 0.04 mg KI 0.10 mg FeCl3 x 6 H2O 0.20 mg MnSO4 x 4 H2O 0.40 mg ZnSO4 x 7 H2O 0.40 mg (NH4)2MoO4 0.20 mg Biotin 0.10 mg Vitamin B12 0.03 mg Distilled water 1000.00 ml For DSM 2118, replace antipyrin by 1 g/l L-phenylalanine. © 2007 DSMZ GmbH - All rights reserved Carrine Blank The pH of this medium should be about 6.9, given the ratio of potassium and sodium phosphates. Phenylobacterium medium An organic-rich, liquid culture medium comprised of antipyrine, potassium phosphate, sodium phosphate, ammonium phosphate, calcium chloride, magnesium sulfate, boric acid, copper sulfate, potassium iodide, ferric trichloride, manganese sulfate, zinc sulfate, ammonium molybdate, biotin, and vitamin B12 (cobalamin). DSM strains: DSMZ Medium 365 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium365.pdf 365. PSEUDOMONAS INDIGOFERA MEDIUM Yeast extract 10.0 g Glucose 5.0 g Na-acetate 0.5 g Agar 12.0 g Distilled water 1000.0 ml Adjust pH to 7.0 - 7.2. Rehydrate and grow lyophilized cells in liquid broth and on agar. © 2007 DSMZ GmbH - All rights reserved Pseudomonas indigofera medium An organic-rich, solid culture medium comprised of yeast extract, glucose, sodium acetate, and agar. DSM strains: DSMZ Medium 369 DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium369.pdf 369. BACILLUS TUSCIAE MEDIUM Adjust medium 261 to pH 4.0. Incubate at 50˚C without agitation under 5% O2, 10% CO2, 45% H2 (v/v). © 2007 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 261, except the pH is decreased to 4.0. Bacillus tusciae medium Carrine Blank DSMZ Medium 368 An organic-rich, liquid culture medium comprised of tryptone, peptone, yeast extract, glucose, tween 80 (polysorbate 80), filtered tomato juice and ethanol. Kunkee medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium368.pdf 368. KUNKEE MEDIUM Tryptone 20.0 g Peptone 5.0 g Yeast extract 5.0 g Glucose 5.0 g Tween 80 0.5 ml Filtered tomato juice 250.0 ml Distilled water 750.0 ml Adjust pH to 5.5. After sterilization aseptically add 10% (v/v) ethanol © 2007 DSMZ GmbH - All rights reserved Carrine Blank mineral solution for DSMZ Medium 321 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium321.pdf 321. METHANOCORPUSCULUM AGGREGANS MEDIUM NH4Cl 1.00 g K2HPO4 x 3 H2O 0.40 g MgCl2 x 6 H2O 0.40 g Mineral solution (see below) 50.00 ml Na-formate 5.00 g Na-acetate 1.00 g Trypticase (BD BBL) 1.00 g Yeast extract 1.00 g Trace element solution (see medium 141) 10.00 ml Sludge fluid (see medium 119) or 5.00 ml Rumen fluid, clarified (see medium 1310) 5.00 ml Resazurin 0.50 mg Na2CO3 1.50 g L-Cysteine-HCl x H2O 0.20 g Na2S x 9 H2O 0.20 g Distilled water 935.00 ml Dissolve ingredients except carbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense medium under same gas atmosphere in anoxic Hungate-type tubes and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use check pH of completed medium and adjust to 6.8 – 7.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Mineral solution: KH2PO4 6.00 g (NH4)2SO4 6.00 g NaCl 12.00 g MgSO4 x 7 H2O 2.60 g CaCl2 x 2 H2O 0.16 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved Carrine Blank An inorganic salts solution comprised of potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, and calcium chloride. DSMZ Medium 311.8 Carrine Blank DSM 26827 is Pelosinus sp. HCF1 Similar to DSMZ Medium 311, except betaine, cysteine, and sulfide are omitted and sodium lactate and dithiothreitol (1,4-dithiothreitol) are added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311.pdf 311. SPOROMUSA MEDIUM NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Betaine x H2O 6.70 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.0. For DSM 3132 and DSM 5090 prepare the medium without betaine; add 5.00 g/l Dfructose to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 4440 use 1.35 g/l betaine; cysteine and sulfide must be replaced by 0.15 g/l DLdithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 6539, DSM 6540, and DSM 17108 prepare the medium without betaine; add 2.00 g/l D-glucose to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 10669, DSM 12793, DSM 12797, and DSM 12993 prepare the medium without betaine; add 5.00 g/l D-fructose to the autoclaved medium from a filter-sterilized anoxic stock solution. Adjust pH of completed medium to 6.5. For DSM 14980 prepare the medium without betaine; add 8.00 g/l D-glucose and 2.00 g/l Na-pyruvate to the autoclaved medium from filter-sterilized anoxic stock solutions. For DSM 16652 prepare the medium without betaine; add 1.11 g/l N-acetyl-D-glucosamine to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 17189 and DSM 17285 prepare the medium without betaine; add 3.60 g/l Dmannitol to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.30 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 26827 prepare the medium without betaine; add 2.50 g/l Na-(DL)-lactate to the autoclaved medium from a separately sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 311.8 -< for DSM 26827 V-8 juice Sodium content is 640 mg per 240 mL (= 0.267% Na+, or =0.68% NaCl). http://www.campbellfoodservice.com/details.aspx?code=61 INGREDIENTS RECONSTITUTED VEGETABLE JUICE BLEND (WATER AND CONCENTRATED JUICES OF TOMATOES, CARROTS, CELERY, BEETS, PARSLEY, LETTUCE, WATERCRESS, SPINACH), CONTAINS LESS THAN 2% OF: SALT, VITAMIN C (ASCORBIC ACID), NATURAL FLAVORING, CITRIC ACID. Carrine Blank An undefined organic chemical mixture comprised of tomato juice (the fruit of Solanum lycopersicum), carrot juice (the root of Daucus carota), celery juice (the stems of Apium graveolens Dulce Group), beet juice (the root of Beta vulgaris), parsley juice (the leaf of Petroselinum crispum), lettuce juice (the leaf of Lactuca sativa), watercress juice (leaf of Nasturtium officinale), and spinach juice (leaf of Spinacia oleracea). Also contains salt (sodium chloride), vitamin C (ascorbic acid), natural flavoring (flavoring agent), and citric acid. Tryptic Soy Broth without Dextrose Carrine Blank From: Bacto™ Tryptic Soy Broth/Trypticase™ Soy Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Tryptic (Trypticase) Soy Broth (Soybean-Casein Digest Medium) is a general purpose medium used in qualitative procedures for the cultivation of fastidious and nonfastidious microorganisms from a variety of clinical and nonclinical specimens. Principles of the Procedure Enzymatic digests of casein and soybean provide amino acids and other complex nitrogenous substances. Dextrose is an energy source. Sodium chloride maintains the osmotic equilibrium. Dibasic potassium phosphate acts as a buffer to control pH. The addition of 6.5% sodium chloride to Trypticase Soy Broth permits the differentiation of salt-tolerant enterococci, which are resistant to the high salt content, from the salt-intolerant S. bovis group and other streptococcal species. At this concentration, the sodium chloride is a selective agent that interferes with membrane permeability and osmotic and electrokinetic equilibria.4 Fildes Enrichment is a peptic digest of sheep blood that supplies the X (hemin) and V (nicotinamide adenine dinucleotide, NAD) factors necessary for the growth of H. influenzae. Dextrose is omitted from the formula for Tryptic Soy Broth without Dextrose to permit use of the medium in fermentation studies. The carbohydrate concentration used most frequently in fermentation reactions is 0.5% or 1%. Tryptic Soy Broth and Trypticase Soy Broth are provided as prepared media in a variety of bottle styles. In addition, Tryptic Soy Broth is provided as a Sterile Pack Bottle; i.e., the bottle has been terminally sterilized inside of autoclavable double-bags. All varieties of bottled TSB conform with requirements for Ready- To-Use Media as described in the USP. Formulae Bacto™ Tryptic Soy Broth (Soybean-Casein Digest Medium) Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 17.0 g Papaic Digest of Soybean............................................. 3.0 g Dextrose...................................................................... 2.5 g Sodium Chloride.......................................................... 5.0 g Dipotassium Phosphate................................................ 2.5 g pH 7.3 ± 0.2 BBL™ Trypticase™ Soy Broth (Soybean-Casein Digest Broth) Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 17.0 g Papaic Digest of Soybean............................................. 3.0 g Sodium Chloride.......................................................... 5.0 g Dipotassium Phosphate................................................ 2.5 g Dextrose...................................................................... 2.5 g pH 7.3 ± 0.2 Bacto™ Tryptic Soy Broth without Dextrose Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 17.0 g Enzymatic Digest of Soybean Meal............................... 3.0 g Sodium Chloride.......................................................... 5.0 g Dipotassium Phosphate................................................ 2.5 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.2 An organic-rich, liquid culture medium comprised of pancreatic digest of casein (casitone), enzymatic digest of soybean meal (soy peptone), sodium chloride, and dipotassium phosphate (potassium dibasic phosphate). trace elements solution for DSMZ Medium 315 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium315.pdf 315. BACTEROIDES CELLULOSOLVENS MEDIUM NH4Cl 0.68 g K2HPO4 0.30 g KH2PO4 0.18 g (NH4)2SO4 0.15 g MgSO4 x 7 H2O 0.12 g CaCl2 x 2 H2O 0.06 g FeSO4 x 7 H2O 0.02 g Trace element solution (see below) 10.00 ml Vitamin solution (see below) 10.00 ml Cellobiose 5.00 g or Cellulose (i.e. MN 300) 5.00 g Resazurin 1.00 mg NaHCO3 2.00 g Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Adjust pH to 7.0. Gas atmosphere: 80% N2 + 20% CO2. Filter sterilize cellobiose separately. Trace element solution: Nitrilotriacetic acid 1.500 g MgSO4 x 7 H2O 3.000 g MnSO4 x H2O 0.500 g NaCl 1.000 g FeSO4 x 7 H2O 0.100 g CoSO4 x 7 H2O 0.180 g CaCl2 x 2 H2O 0.100 g ZnSO4 x 7 H2O 0.180 g CuSO4 x 5 H2O 0.010 g KAl(SO4)2 x 12 H2O 0.020 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.010 g NiCl2 x 6 H2O 0.025 g Na2SeO3 x 5 H2O 0.300 mg Distilled water 1000.000 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). © 2009 DSMZ GmbH - All rights reserved A trace elements solution comprised of nitrilotriacetic acid, magnesium sulfate, manganese sulfate, sodium chloride, ferrous sulfate, cobalt sulfate, calcium chloride, zinc sulfate, copper sulfate, potassium aluminum sulfate, boric acid, sodium molybdate, nickel chloride, and sodium selenite. Carrine Blank DSMZ Medium 311.7 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311.pdf 311. SPOROMUSA MEDIUM NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Betaine x H2O 6.70 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.0. For DSM 3132 and DSM 5090 prepare the medium without betaine; add 5.00 g/l Dfructose to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 4440 use 1.35 g/l betaine; cysteine and sulfide must be replaced by 0.15 g/l DLdithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 6539, DSM 6540, and DSM 17108 prepare the medium without betaine; add 2.00 g/l D-glucose to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 10669, DSM 12793, DSM 12797, and DSM 12993 prepare the medium without betaine; add 5.00 g/l D-fructose to the autoclaved medium from a filter-sterilized anoxic stock solution. Adjust pH of completed medium to 6.5. For DSM 14980 prepare the medium without betaine; add 8.00 g/l D-glucose and 2.00 g/l Na-pyruvate to the autoclaved medium from filter-sterilized anoxic stock solutions. For DSM 16652 prepare the medium without betaine; add 1.11 g/l N-acetyl-D-glucosamine to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 17189 and DSM 17285 prepare the medium without betaine; add 3.60 g/l Dmannitol to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.30 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 26827 prepare the medium without betaine; add 2.50 g/l Na-(DL)-lactate to the autoclaved medium from a separately sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 311.7 -< for DSM 17189 and DSM 17285 Similar to DSMZ Medium 311, except betaine, sulfide, and cysteine are omitted and D-mannitol and dithiothreitol are added. DSM 17189 is Sporomusa intestinalis DSM 17285 is Sporomusa (not in TaxBrowser or StrainInfo) DSMZ Medium 311.6 DSM 16652 is Sporomusa rhizae DSMZ Medium 311.6 -< for DSM 16652 Similar to DSMZ Medium 311, except betaine is omitted and N-acetyl-D-glucosamine is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311.pdf 311. SPOROMUSA MEDIUM NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Betaine x H2O 6.70 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.0. For DSM 3132 and DSM 5090 prepare the medium without betaine; add 5.00 g/l Dfructose to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 4440 use 1.35 g/l betaine; cysteine and sulfide must be replaced by 0.15 g/l DLdithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 6539, DSM 6540, and DSM 17108 prepare the medium without betaine; add 2.00 g/l D-glucose to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 10669, DSM 12793, DSM 12797, and DSM 12993 prepare the medium without betaine; add 5.00 g/l D-fructose to the autoclaved medium from a filter-sterilized anoxic stock solution. Adjust pH of completed medium to 6.5. For DSM 14980 prepare the medium without betaine; add 8.00 g/l D-glucose and 2.00 g/l Na-pyruvate to the autoclaved medium from filter-sterilized anoxic stock solutions. For DSM 16652 prepare the medium without betaine; add 1.11 g/l N-acetyl-D-glucosamine to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 17189 and DSM 17285 prepare the medium without betaine; add 3.60 g/l Dmannitol to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.30 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 26827 prepare the medium without betaine; add 2.50 g/l Na-(DL)-lactate to the autoclaved medium from a separately sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 311.5 Carrine Blank Similar to DSMZ Medium 311, except betaine is omitted and glucose and sodium pyruvate are added. DSMZ Medium 311.5 -< for DSM 14980 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311.pdf 311. SPOROMUSA MEDIUM NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Betaine x H2O 6.70 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.0. For DSM 3132 and DSM 5090 prepare the medium without betaine; add 5.00 g/l Dfructose to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 4440 use 1.35 g/l betaine; cysteine and sulfide must be replaced by 0.15 g/l DLdithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 6539, DSM 6540, and DSM 17108 prepare the medium without betaine; add 2.00 g/l D-glucose to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 10669, DSM 12793, DSM 12797, and DSM 12993 prepare the medium without betaine; add 5.00 g/l D-fructose to the autoclaved medium from a filter-sterilized anoxic stock solution. Adjust pH of completed medium to 6.5. For DSM 14980 prepare the medium without betaine; add 8.00 g/l D-glucose and 2.00 g/l Na-pyruvate to the autoclaved medium from filter-sterilized anoxic stock solutions. For DSM 16652 prepare the medium without betaine; add 1.11 g/l N-acetyl-D-glucosamine to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 17189 and DSM 17285 prepare the medium without betaine; add 3.60 g/l Dmannitol to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.30 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 26827 prepare the medium without betaine; add 2.50 g/l Na-(DL)-lactate to the autoclaved medium from a separately sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSM 14980 is Moorella mulderi DSMZ Medium 311.4 Similar to DSMZ Medium 311, except betaine is omitted and fructose is added. The pH is decreased to 6.5. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311.pdf 311. SPOROMUSA MEDIUM NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Betaine x H2O 6.70 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.0. For DSM 3132 and DSM 5090 prepare the medium without betaine; add 5.00 g/l Dfructose to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 4440 use 1.35 g/l betaine; cysteine and sulfide must be replaced by 0.15 g/l DLdithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 6539, DSM 6540, and DSM 17108 prepare the medium without betaine; add 2.00 g/l D-glucose to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 10669, DSM 12793, DSM 12797, and DSM 12993 prepare the medium without betaine; add 5.00 g/l D-fructose to the autoclaved medium from a filter-sterilized anoxic stock solution. Adjust pH of completed medium to 6.5. For DSM 14980 prepare the medium without betaine; add 8.00 g/l D-glucose and 2.00 g/l Na-pyruvate to the autoclaved medium from filter-sterilized anoxic stock solutions. For DSM 16652 prepare the medium without betaine; add 1.11 g/l N-acetyl-D-glucosamine to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 17189 and DSM 17285 prepare the medium without betaine; add 3.60 g/l Dmannitol to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.30 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 26827 prepare the medium without betaine; add 2.50 g/l Na-(DL)-lactate to the autoclaved medium from a separately sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 10669 is Sporomusa silvacetica DSM 12793 is Thermicanus aegyptius DSM 12793 DSM 12797 is Moorella thermoacetica DSM 12993 is Moorella thermoacetica DSMZ Medium 311.4 -< for DSM 10669, DSM 12793, DSM 12797, and DSM 12993 DSMZ Medium 311.3 DSM 6539 is Terrisporobacter mayombei DSM 6540 is Acetonema longum DSM 6540 DSM 17108 is Pelosinus fermentans DSM 17108 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311.pdf 311. SPOROMUSA MEDIUM NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Betaine x H2O 6.70 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.0. For DSM 3132 and DSM 5090 prepare the medium without betaine; add 5.00 g/l Dfructose to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 4440 use 1.35 g/l betaine; cysteine and sulfide must be replaced by 0.15 g/l DLdithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 6539, DSM 6540, and DSM 17108 prepare the medium without betaine; add 2.00 g/l D-glucose to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 10669, DSM 12793, DSM 12797, and DSM 12993 prepare the medium without betaine; add 5.00 g/l D-fructose to the autoclaved medium from a filter-sterilized anoxic stock solution. Adjust pH of completed medium to 6.5. For DSM 14980 prepare the medium without betaine; add 8.00 g/l D-glucose and 2.00 g/l Na-pyruvate to the autoclaved medium from filter-sterilized anoxic stock solutions. For DSM 16652 prepare the medium without betaine; add 1.11 g/l N-acetyl-D-glucosamine to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 17189 and DSM 17285 prepare the medium without betaine; add 3.60 g/l Dmannitol to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.30 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 26827 prepare the medium without betaine; add 2.50 g/l Na-(DL)-lactate to the autoclaved medium from a separately sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 311, except betaine, cysteine, and sulfide are omitted and glucose and dithiothreitol are added. Carrine Blank DSMZ Medium 311.3 -< for DSM 6539, DSM 6540, and DSM 17108 DSMZ Medium 311.2 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311.pdf 311. SPOROMUSA MEDIUM NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Betaine x H2O 6.70 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.0. For DSM 3132 and DSM 5090 prepare the medium without betaine; add 5.00 g/l Dfructose to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 4440 use 1.35 g/l betaine; cysteine and sulfide must be replaced by 0.15 g/l DLdithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 6539, DSM 6540, and DSM 17108 prepare the medium without betaine; add 2.00 g/l D-glucose to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 10669, DSM 12793, DSM 12797, and DSM 12993 prepare the medium without betaine; add 5.00 g/l D-fructose to the autoclaved medium from a filter-sterilized anoxic stock solution. Adjust pH of completed medium to 6.5. For DSM 14980 prepare the medium without betaine; add 8.00 g/l D-glucose and 2.00 g/l Na-pyruvate to the autoclaved medium from filter-sterilized anoxic stock solutions. For DSM 16652 prepare the medium without betaine; add 1.11 g/l N-acetyl-D-glucosamine to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 17189 and DSM 17285 prepare the medium without betaine; add 3.60 g/l Dmannitol to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.30 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 26827 prepare the medium without betaine; add 2.50 g/l Na-(DL)-lactate to the autoclaved medium from a separately sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSM 4440 is Sporomusa termitida DSMZ Medium 311.2 -< for DSM 4440 Similar to DSMZ Medium 311, except the concentration of betaine in decreased, sulfide is omitted, and dithiothreitol is added. DSMZ Medium 311.1 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311.pdf 311. SPOROMUSA MEDIUM NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Betaine x H2O 6.70 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.0. For DSM 3132 and DSM 5090 prepare the medium without betaine; add 5.00 g/l Dfructose to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 4440 use 1.35 g/l betaine; cysteine and sulfide must be replaced by 0.15 g/l DLdithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 6539, DSM 6540, and DSM 17108 prepare the medium without betaine; add 2.00 g/l D-glucose to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 10669, DSM 12793, DSM 12797, and DSM 12993 prepare the medium without betaine; add 5.00 g/l D-fructose to the autoclaved medium from a filter-sterilized anoxic stock solution. Adjust pH of completed medium to 6.5. For DSM 14980 prepare the medium without betaine; add 8.00 g/l D-glucose and 2.00 g/l Na-pyruvate to the autoclaved medium from filter-sterilized anoxic stock solutions. For DSM 16652 prepare the medium without betaine; add 1.11 g/l N-acetyl-D-glucosamine to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 17189 and DSM 17285 prepare the medium without betaine; add 3.60 g/l Dmannitol to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.30 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 26827 prepare the medium without betaine; add 2.50 g/l Na-(DL)-lactate to the autoclaved medium from a separately sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSM 3132 is Sporomusa acidovorans DSM 5090 is Sporomusa malonica DSM 5090 DSMZ Medium 311.1 -< for DSM 3132 and DSM 5090 Similar to DSMZ Medium 311, except betaine is omitted and D-fructose is added. DSMZ Medium 311 Sporomusa medium An organic-rich, liquid culture medium comprised of ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, selenite-tungstate, yeast extract, casitone, betaine, resazurin, potassium phosphate, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311.pdf 311. SPOROMUSA MEDIUM NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Betaine x H2O 6.70 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.0. For DSM 3132 and DSM 5090 prepare the medium without betaine; add 5.00 g/l Dfructose to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 4440 use 1.35 g/l betaine; cysteine and sulfide must be replaced by 0.15 g/l DLdithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 6539, DSM 6540, and DSM 17108 prepare the medium without betaine; add 2.00 g/l D-glucose to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 10669, DSM 12793, DSM 12797, and DSM 12993 prepare the medium without betaine; add 5.00 g/l D-fructose to the autoclaved medium from a filter-sterilized anoxic stock solution. Adjust pH of completed medium to 6.5. For DSM 14980 prepare the medium without betaine; add 8.00 g/l D-glucose and 2.00 g/l Na-pyruvate to the autoclaved medium from filter-sterilized anoxic stock solutions. For DSM 16652 prepare the medium without betaine; add 1.11 g/l N-acetyl-D-glucosamine to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 17189 and DSM 17285 prepare the medium without betaine; add 3.60 g/l Dmannitol to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.30 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 26827 prepare the medium without betaine; add 2.50 g/l Na-(DL)-lactate to the autoclaved medium from a separately sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved DSM strains: mineral solution for DSMZ Medium 317 An inorganic salts solution comprised of potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, and calcium chloride. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium317.pdf 317. METHANOSARCINA (MP) MEDIUM NH4Cl 1.00 g K2HPO4 0.40 g MgCl2 x 6 H2O 0.20 g Mineral solution (see below) 50.00 ml Trace element solution (see medium 141) 10.00 ml Yeast extract 1.00 g Resazurin 0.50 mg Na2CO3 1.00 g Methanol 5.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.25 g Distilled water 950.00 ml Dissolve ingredients except carbonate, methanol, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under the same gas atmosphere in anoxic Hungate-type tubes or serum vilas and autoclave. Add methanol, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 6.8 - 7.0. Minerals solution: KH2PO4 6.00 g (NH4)2SO4 6.00 g NaCl 12.00 g MgSO4 x 7 H2O 2.60 g CaCl2 x 2 H2O 0.16 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 332 An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, magnesium chloride, sodium formate, sodium acetate, trypticase peptone, yeast extract, resazurin, L-cysteine hydrochloride, sodium carbonate, and sodium sulfide. Prepared under an atmosphere of dihygrogen, carbon dioxide, and dinitrogen. DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium332.pdf 332. METHANOGENIUM BOURGENSE MEDIUM NH4Cl 1.0 g K2HPO4 x 3 H2O 0.4 g MgCl2 x 6 H2O 0.1 g Na-formate 5.0 g Na-acetate 1.0 g Trypticase peptone (BD BBL) 1.0 g Yeast extract (OXOID) 1.0 g Resazurin 0.5 mg L-Cysteine-HCl x H2O 0.5 g Na2CO3 1.5 g Na2S x 9 H2O 0.2 g Distilled water 1000.0 ml Dissolve ingredients except cysteine, carbonate and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve cysteine, then dispense medium under same gas atmosphere in anoxic Hungate-type tubes and autoclave. Add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture and sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. Note: After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. © 2014 DSMZ GmbH - All rights reserved Methanogenium bourgense medium DSMZ Medium 331 Carrine Blank DSM strains: An organic-rich, liquid culture medium comprised of calcium chloride, ammonium chloride, manganese chloride, trace elements, vitamins, resazurin, yeast extract, glycine, potassium phosphate, sodium phosphate, sodium bicarbonate, and sodium sulfide. Prepared under an atmosphere of dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium331.pdf 331. ACETOBACTEROIDES GLYCINOPHILUS MEDIUM CaCl2 x 2 H2O 0.13 g NH4Cl 1.00 g MgCl2 x 6 H2O 0.20 g Trace elements (see medium 144) 10.00 ml Vitamin solution (see medium 141) 5.00 ml Resazurin 1.00 mg Yeast extract 0.50 g Glycine 1.50 g KH2PO4 3.00 g Na2HPO4 5.80 g NaHCO3 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Adjust pH to 7.2 - 7.4 with NaOH. Prepare the medium anaerobically under 100% nitrogen. Prepare concentrated solutions each of yeast extract, glycine, phosphates, NaHCO3 and Na2S separately and sterilize under nitrogen by autoclaving. © 2007 DSMZ GmbH - All rights reserved Acetobacteroides glycinophilus medium DSMZ Medium 333 Carrine Blank Thiobacillus tepidarius medium A minerals-salts, solid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, trace elements, potassium tetrathionate, bromocresol purple, and agar. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium333.pdf 333. THIOBACILLUS TEPIDARIUS MEDIUM KH2PO4 4.0 g K2HPO4 4.0 g NH4Cl 0.4 g MgSO4 x 7 H2O 0.8 g Trace elements (see below) 10.0 ml K2S4O6 3.0 g Bromocresol purple (saturated aqu. sol.) 2.0 ml Bacto Agar DIFCO (for solid medium) 10.0 g Distilled water 1000.0 ml The phosphates are sterilized separately in 1/10 of medium volume and mixed with the other salts when cool. Adjust pH of final medium to 6.9. For agar medium autoclave the phosphates (in 100 ml), other salts (in 300 ml), and agar (in 600 ml) separately. Trace element solution: Na2 - EDTA 50.0 g ZnSO4 x 7 H2O 11.0 g CaCl2 x 2 H2O 7.34 g MnCl2 x 4 H2O 2.5 g CoCl2 x 6 H2O 0.5 g (NH4)6 Mo7O24 x 4 H2O 0.5 g FeSO4 x 7 H2O 5.0 g CuSO4 x 5 H2O 0.2 g NaOH ca 11.0 g Distilled water 1000.0 ml First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using NaOH; then add other compounds. Adjust pH of final solution to 6.0. © 2007 DSMZ GmbH - All rights reserved DSM strains: trace elements solution for DSMZ Medium 333 A trace elements solution comprised of disodium EDTA, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, hexaammonium heptamolybdate, ferrous sulfate, copper chloride, and sodium hydroxide. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium333.pdf 333. THIOBACILLUS TEPIDARIUS MEDIUM KH2PO4 4.0 g K2HPO4 4.0 g NH4Cl 0.4 g MgSO4 x 7 H2O 0.8 g Trace elements (see below) 10.0 ml K2S4O6 3.0 g Bromocresol purple (saturated aqu. sol.) 2.0 ml Bacto Agar DIFCO (for solid medium) 10.0 g Distilled water 1000.0 ml The phosphates are sterilized separately in 1/10 of medium volume and mixed with the other salts when cool. Adjust pH of final medium to 6.9. For agar medium autoclave the phosphates (in 100 ml), other salts (in 300 ml), and agar (in 600 ml) separately. Trace element solution: Na2 - EDTA 50.0 g ZnSO4 x 7 H2O 11.0 g CaCl2 x 2 H2O 7.34 g MnCl2 x 4 H2O 2.5 g CoCl2 x 6 H2O 0.5 g (NH4)6 Mo7O24 x 4 H2O 0.5 g FeSO4 x 7 H2O 5.0 g CuSO4 x 5 H2O 0.2 g NaOH ca 11.0 g Distilled water 1000.0 ml First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using NaOH; then add other compounds. Adjust pH of final solution to 6.0. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 334.2 Similar to DSMZ Medium 334, except yeast extract and trypticase peptone are added. DSM 17206 is not in www.dsmz.de. Is Methanosaeta harundinacea 8Ac according to Strain Info. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium334.pdf 334. METHANOTHRIX MEDIUM Solution A: KH2PO4 0.22 g Na2HPO4 x 2 H2O 0.86 g Na-acetate 6.80 g Na2-EDTA 1.00 mg Trace elements solution (see medium 144) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: NaHCO3 4.00 g Distilled water 40.00 ml Solution C: NaCl 0.30 g CaCl2 x 2 H2O 0.11 g MgCl2 x 6 H2O 0.10 g Distilled water 10.00 ml Solution D: NH4Cl 0.30 g Distilled water 10.00 ml Solution E: Vitamins solution (see medium 141, but 10x conc.) 1.00 ml Solution F: Na2S x 9 H2O 0.50 g Distilled water 10.00 ml Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, dispense under same gas atmosphere in anoxic serum vials (e.g., 20 ml medium in 50 ml serum bottles) and autoclave. Prior to inoculation complete the medium by adding appropriate amounts of sterile solutions C, D, E and F prepared under 100% N2 gas and solution B prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins are sterilized by filtration. Adjust the pH of the complete medium to 7.3 – 7.5. Note: Use 20% (v/v) inoculum. For DSM 3870, DSM 4774 and DSM 6194 supplement medium with 10.00 ml/l of a coenzyme M (2-mercaptoethanesulfonate) solution (1.42% w/v, prepared under N2) and adjust pH of final medium to 7.0. For DSM 17206 supplement medium with 0.50 g/l yeast extract and 0.50 g/l Trypticase peptone added after autoclaving from sterile anoxic stock solutions. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 334.2 -< for DSM 17206 DSMZ Medium 334.1 Similar to DSMZ Medium 334, except coenzyme M is added, and the pH is decreased to 7.0. Carrine Blank DSM 3870 is Methanosaeta DSM 4774 is Methanosaeta thermophila DSM 6194 is Methanosaeta thermophila PT http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium334.pdf 334. METHANOTHRIX MEDIUM Solution A: KH2PO4 0.22 g Na2HPO4 x 2 H2O 0.86 g Na-acetate 6.80 g Na2-EDTA 1.00 mg Trace elements solution (see medium 144) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: NaHCO3 4.00 g Distilled water 40.00 ml Solution C: NaCl 0.30 g CaCl2 x 2 H2O 0.11 g MgCl2 x 6 H2O 0.10 g Distilled water 10.00 ml Solution D: NH4Cl 0.30 g Distilled water 10.00 ml Solution E: Vitamins solution (see medium 141, but 10x conc.) 1.00 ml Solution F: Na2S x 9 H2O 0.50 g Distilled water 10.00 ml Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, dispense under same gas atmosphere in anoxic serum vials (e.g., 20 ml medium in 50 ml serum bottles) and autoclave. Prior to inoculation complete the medium by adding appropriate amounts of sterile solutions C, D, E and F prepared under 100% N2 gas and solution B prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins are sterilized by filtration. Adjust the pH of the complete medium to 7.3 – 7.5. Note: Use 20% (v/v) inoculum. For DSM 3870, DSM 4774 and DSM 6194 supplement medium with 10.00 ml/l of a coenzyme M (2-mercaptoethanesulfonate) solution (1.42% w/v, prepared under N2) and adjust pH of final medium to 7.0. For DSM 17206 supplement medium with 0.50 g/l yeast extract and 0.50 g/l Trypticase peptone added after autoclaving from sterile anoxic stock solutions. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 334.1 -< for DSM 3870, DSM 4774 and DSM 6194 DSMZ Medium 334 Methanothrix medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium334.pdf 334. METHANOTHRIX MEDIUM Solution A: KH2PO4 0.22 g Na2HPO4 x 2 H2O 0.86 g Na-acetate 6.80 g Na2-EDTA 1.00 mg Trace elements solution (see medium 144) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: NaHCO3 4.00 g Distilled water 40.00 ml Solution C: NaCl 0.30 g CaCl2 x 2 H2O 0.11 g MgCl2 x 6 H2O 0.10 g Distilled water 10.00 ml Solution D: NH4Cl 0.30 g Distilled water 10.00 ml Solution E: Vitamins solution (see medium 141, but 10x conc.) 1.00 ml Solution F: Na2S x 9 H2O 0.50 g Distilled water 10.00 ml Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, dispense under same gas atmosphere in anoxic serum vials (e.g., 20 ml medium in 50 ml serum bottles) and autoclave. Prior to inoculation complete the medium by adding appropriate amounts of sterile solutions C, D, E and F prepared under 100% N2 gas and solution B prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins are sterilized by filtration. Adjust the pH of the complete medium to 7.3 – 7.5. Note: Use 20% (v/v) inoculum. For DSM 3870, DSM 4774 and DSM 6194 supplement medium with 10.00 ml/l of a coenzyme M (2-mercaptoethanesulfonate) solution (1.42% w/v, prepared under N2) and adjust pH of final medium to 7.0. For DSM 17206 supplement medium with 0.50 g/l yeast extract and 0.50 g/l Trypticase peptone added after autoclaving from sterile anoxic stock solutions. © 2015 DSMZ GmbH - All rights reserved Carrine Blank A minerals-salts, liquid culture medium comprised of potassium phosphate, sodium phosphate, sodium acetate, disodium EDTA, trace elements, resazurin, sodium bicarbonate, sodium chloride, calcium chloride, magnesium chloride, ammonium chloride, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 335 DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium335.pdf 335. EUBACTERIUM OXIDOREDUCENS MEDIUM Solution A: Mineral solution (see below) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 2.00 g Crotonic acid 5.00 g Vitamin solution (see medium 503) 1.00 ml Resazurin 1.00 mg Distilled water 840.00 ml Adjust pH to 6.9 before autoclaving. Solution B: NaHCO3 5.00 g Distilled water 100.00 ml Solution C: L-Cysteine-HCl x H2O 0.24 g Distilled water 10.00 ml Solution D: Na2S x 9 H2O 78.00 mg Distilled water 1.00 ml Prepare solution A under 80% N2 and 20% CO2 gas mixture, distribute 8.9 ml per Hungate-type tube under same gas atmosphere and autoclave. Filter sterilize NaHCO3 solution and gas for 20 min with 80% N2 and 20% CO2 gas mixture. Autoclave cysteine and sulfide under 100% N2 gas. Complete medium by adding the solutions B, C, and D with a syringe to solution A. Adjust final pH of medium to 7.0 - 7.5. Mineral solution: KH2PO4 10.0 g MgCl2 x 6 H2O 6.6 g NaCl 8.0 g NH4Cl 8.0 g CaCl2 x 2 H2O 1.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of mineral solution, trace elements, yeast extract, crotonic acid, vitamins, resazurin, sodium bicarbonate, L-cysteine hydrochlrodie, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank Eubacterium oxidoreducens medium mineral solution for DSMZ Medium 335 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium335.pdf 335. EUBACTERIUM OXIDOREDUCENS MEDIUM Solution A: Mineral solution (see below) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 2.00 g Crotonic acid 5.00 g Vitamin solution (see medium 503) 1.00 ml Resazurin 1.00 mg Distilled water 840.00 ml Adjust pH to 6.9 before autoclaving. Solution B: NaHCO3 5.00 g Distilled water 100.00 ml Solution C: L-Cysteine-HCl x H2O 0.24 g Distilled water 10.00 ml Solution D: Na2S x 9 H2O 78.00 mg Distilled water 1.00 ml Prepare solution A under 80% N2 and 20% CO2 gas mixture, distribute 8.9 ml per Hungate-type tube under same gas atmosphere and autoclave. Filter sterilize NaHCO3 solution and gas for 20 min with 80% N2 and 20% CO2 gas mixture. Autoclave cysteine and sulfide under 100% N2 gas. Complete medium by adding the solutions B, C, and D with a syringe to solution A. Adjust final pH of medium to 7.0 - 7.5. Mineral solution: KH2PO4 10.0 g MgCl2 x 6 H2O 6.6 g NaCl 8.0 g NH4Cl 8.0 g CaCl2 x 2 H2O 1.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved An inorganic salts solution comprised of potassium phosphate, magnesium chloride, sodium chloride, ammonium chloride, and calcium chloride. DSMZ Medium 339.3 Similar to DSMZ Medium 339, except dithiothreitol is added and the pH is increased to 7.4. Carrine Blank DSM 23669 is Acetatifactor muris DSMZ Medium 339.3 -< for DSM 23669 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium339.pdf 339. WILKINS-CHALGREN ANAEROBE BROTH Dissolve dehydrated Wilkins-Chalgren medium (Oxoid CM0643) and resazurin (0.5 mg/l) in distilled water, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving check pH and adjust to pH 6.8, if necessary. For DSM 5676 supplement medium with 10.0 g/l D-glucose. For DSM 12679 and DSM 12858 use medium at half-strength (16.5 g/l). For DSM 23669 supplement medium after autoclaving with 0.2 g/l dithiothreitol (DTT) added from a filter-sterilized anoxic stock solution and adjust pH of completed medium to 7.4 © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 339.2 Carrine Blank DSMZ Medium 339.2 -< for DSM 12679 and DSM 12858 DSM 12679 is Caloramator coolhaasii DSM 12858 is Soehngenia saccharolytica DSM 12858 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium339.pdf 339. WILKINS-CHALGREN ANAEROBE BROTH Dissolve dehydrated Wilkins-Chalgren medium (Oxoid CM0643) and resazurin (0.5 mg/l) in distilled water, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving check pH and adjust to pH 6.8, if necessary. For DSM 5676 supplement medium with 10.0 g/l D-glucose. For DSM 12679 and DSM 12858 use medium at half-strength (16.5 g/l). For DSM 23669 supplement medium after autoclaving with 0.2 g/l dithiothreitol (DTT) added from a filter-sterilized anoxic stock solution and adjust pH of completed medium to 7.4 © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 339, except the medium is diluted. DSMZ Medium 339.1 DSMZ Medium 339.1 -< for DSM 5676 Carrine Blank DSM 5676 is [Clostridium] scindens ATCC 35704 Similar to DSMZ Medium 339, except the concentration of glucose is increased. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium339.pdf 339. WILKINS-CHALGREN ANAEROBE BROTH Dissolve dehydrated Wilkins-Chalgren medium (Oxoid CM0643) and resazurin (0.5 mg/l) in distilled water, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving check pH and adjust to pH 6.8, if necessary. For DSM 5676 supplement medium with 10.0 g/l D-glucose. For DSM 12679 and DSM 12858 use medium at half-strength (16.5 g/l). For DSM 23669 supplement medium after autoclaving with 0.2 g/l dithiothreitol (DTT) added from a filter-sterilized anoxic stock solution and adjust pH of completed medium to 7.4 © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 339 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium339.pdf 339. WILKINS-CHALGREN ANAEROBE BROTH Dissolve dehydrated Wilkins-Chalgren medium (Oxoid CM0643) and resazurin (0.5 mg/l) in distilled water, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving check pH and adjust to pH 6.8, if necessary. For DSM 5676 supplement medium with 10.0 g/l D-glucose. For DSM 12679 and DSM 12858 use medium at half-strength (16.5 g/l). For DSM 23669 supplement medium after autoclaving with 0.2 g/l dithiothreitol (DTT) added from a filter-sterilized anoxic stock solution and adjust pH of completed medium to 7.4 © 2015 DSMZ GmbH - All rights reserved Carrine Blank Wilkins-Chalgren Anaerobe Broth (N2/CO2) DSM strains: An organic-rich, liquid culture medium comprised of Wilkins-Chalgren medium, resazurin, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen. DSMZ Medium 339a.4 DSMZ Medium 339a.4 -< for DSM 15243 and DSM 15248 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium339a.pdf 339a. WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) Dissolve dehydrated Wilkins-Chalgren medium (Oxoid CM0643) and resazurin (0.5 mg/l) in distilled water, bring to the boil and cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving supplement medium with 5.0 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.8 with a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 14428 replace D-glucose with 5.0 g/l cellobiose prepared under 100% N2 gas atmosphere and sterilized by filtration. For DSM 14924 adjust final pH of medium to 7.4 using a sterile anoxic stock solution of Na2CO3 prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 15176, DSM 15480, DSM 15481 and DSM 15567 omit D-glucose. For DSM 15243 and DSM 15248 omit Na2CO3 and adjust final pH of medium to 6.0. © 2014 DSMZ GmbH - All rights reserved DSM 15243 is Clostridium carboxidivorans P7 DSM 15248 is Clostridium ragsdalei P11 Similar to DSMZ Medium 339a, except the final pH is decreased to 6.0. Carrine Blank DSMZ Medium 339a.3 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium339a.pdf 339a. WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) Dissolve dehydrated Wilkins-Chalgren medium (Oxoid CM0643) and resazurin (0.5 mg/l) in distilled water, bring to the boil and cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving supplement medium with 5.0 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.8 with a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 14428 replace D-glucose with 5.0 g/l cellobiose prepared under 100% N2 gas atmosphere and sterilized by filtration. For DSM 14924 adjust final pH of medium to 7.4 using a sterile anoxic stock solution of Na2CO3 prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 15176, DSM 15480, DSM 15481 and DSM 15567 omit D-glucose. For DSM 15243 and DSM 15248 omit Na2CO3 and adjust final pH of medium to 6.0. © 2014 DSMZ GmbH - All rights reserved DSM 15176 is Subdoligranulum variabile DSM 15176 DSM 15480 is Hespellia stercorisuis DSM 15480 DSM 15481 is Hespellia porcina DSM 15567 is Mahella australiensis 50-1 BON DSMZ Medium 339a.3 -< for DSM 15176, DSM 15480, DSM 15481 and DSM 15567 Carrine Blank Similar to DSMZ Medium 339a, except D-glucose is omitted. DSMZ Medium 339a.2 Similar to DSMZ Medium 339a, except the pH is increased to 7.4. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium339a.pdf 339a. WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) Dissolve dehydrated Wilkins-Chalgren medium (Oxoid CM0643) and resazurin (0.5 mg/l) in distilled water, bring to the boil and cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving supplement medium with 5.0 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.8 with a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 14428 replace D-glucose with 5.0 g/l cellobiose prepared under 100% N2 gas atmosphere and sterilized by filtration. For DSM 14924 adjust final pH of medium to 7.4 using a sterile anoxic stock solution of Na2CO3 prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 15176, DSM 15480, DSM 15481 and DSM 15567 omit D-glucose. For DSM 15243 and DSM 15248 omit Na2CO3 and adjust final pH of medium to 6.0. © 2014 DSMZ GmbH - All rights reserved DSM 14924 is Caloramator uzoniensis DSMZ Medium 339a.2 -< for DSM 14924 Carrine Blank DSMZ Medium 339a.1 Similar to DSMZ Medium 339a, except D-glucose is omitted and cellobiose added. DSM 14428 is [Clostridium] herbivorans DSMZ Medium 339a.1 -< for DSM 14428 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium339a.pdf 339a. WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) Dissolve dehydrated Wilkins-Chalgren medium (Oxoid CM0643) and resazurin (0.5 mg/l) in distilled water, bring to the boil and cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving supplement medium with 5.0 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.8 with a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 14428 replace D-glucose with 5.0 g/l cellobiose prepared under 100% N2 gas atmosphere and sterilized by filtration. For DSM 14924 adjust final pH of medium to 7.4 using a sterile anoxic stock solution of Na2CO3 prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 15176, DSM 15480, DSM 15481 and DSM 15567 omit D-glucose. For DSM 15243 and DSM 15248 omit Na2CO3 and adjust final pH of medium to 6.0. © 2014 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 339a An organic-rich, liquid culture medium comprised of Wilkins-Chalgren medium, resazurin, L-cysteine hydrochloride, and D-glucose. Prepared under an atmosphere of dinitrogen and carbon dioxide. Wilkins-Chalgren Anaerobe Broth (N2/CO2) DSM strains: Wilkins-Chalgren Anaerobe Broth Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium339a.pdf 339a. WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) Dissolve dehydrated Wilkins-Chalgren medium (Oxoid CM0643) and resazurin (0.5 mg/l) in distilled water, bring to the boil and cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving supplement medium with 5.0 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.8 with a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 14428 replace D-glucose with 5.0 g/l cellobiose prepared under 100% N2 gas atmosphere and sterilized by filtration. For DSM 14924 adjust final pH of medium to 7.4 using a sterile anoxic stock solution of Na2CO3 prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 15176, DSM 15480, DSM 15481 and DSM 15567 omit D-glucose. For DSM 15243 and DSM 15248 omit Na2CO3 and adjust final pH of medium to 6.0. © 2014 DSMZ GmbH - All rights reserved potato flour Carrine Blank Flour made from ground, dried potato tubers. DSMZ Medium 383.13 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 383.13 -< for DSM 14454 DSM 14454 is delta proteobacterium NaphS2 Similar to DSMZ Medium 383, except naphthalene and heptamethylnonane (isocetane) are added (or sodium pyruvate is added). DSMZ Medium 383.12 DSM 13036 is Desulfobacterium sp. BSv41 DSMZ Medium 383.12 -< for DSM 13036 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 383, except betaine (glycine betaine) is added. DSMZ Medium 383.11 DSM 12888 is Desulfotignum balticum http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 383.11 -< for DSM 12888 Similar to DSMZ Medium 383, except sodium buytrate and caproic acid (hexanoic acid) is added. DSMZ Medium 383.10 DSM 12861 is delta proteobacterium S2550 DSM 12883 is delta proteobacterium S2551 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 383.10 -< for DSM 12861 and DSM 12883 Carrine Blank Similar to DSMZ Medium 383, except veralic acid is added. DSMZ Medium 383.9 Carrine Blank DSM 9705 is Desulfofustis glycolicus DSM 9705 Similar to DSMZ Medium 383, except sodium glycolate is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 383.9 -< for DSM 9705 DSMZ Medium 383.8 DSM 8541 is Desulfococcus sp. DSM 8541 DSM 10085 is Desulfospira joergensenii DSM 10085 DSM 16918 is Desulfovibrio sp. M2 DSMZ Medium 383.8 -< for DSM 8541, DSM 10085 and DSM 16918 Similar to DSMZ Medium 383, except sodium pyruvate is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 383.7 DSM 8540 is Desulfobacterium DSMZ Medium 383.7 -< for DSM 8540 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 383, except sodium 4-hydroxybenzoate is added. DSMZ Medium 383.6 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 7044 is Desulfotignum balticum DSM 7044 DSM 7120 is Desulfobacterium DSM 7467 is Desulfobacula toluolica Tol2 DSM 12567 is sulfate-reducing bacterium mXyS1 DSM 13228 is Desulfosarcina ovata DSMZ Medium 383.6 -< for DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228 Similar to DSMZ Medium 383, except sodium benzoate, seven vitamins solution, and yeast extract are added. DSMZ Medium 383.5 DSM 5091 is Malonomonas rubra DSM 5091 DSM 9788 is Malonomonas rubra Similar to DSMZ Medium 383, except malonic acid is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 383.5 -< for DSM 5091 and DSM 9788 Carrine Blank DSMZ Medium 383.4 DSMZ Medium 383.4 -< for DSM 4661 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved DSM 4661 is an unclassified bacterium with no sequences. Similar to DSMZ Medium 383, except resorcinol is added. DSMZ Medium 383.3 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved DSM 3384 is Desulfobacula phenolica DSMZ Medium 383.3 -< for DSM 3384 Similar to DSMZ Medium 383, except sodium benzoate and phenol are added. Carrine Blank DSMZ Medium 383.2 Similar to DSMZ Medium 383, except indole is added. The concentrations of sodium chloride and magnesium dichloride are increased. Carrine Blank DSM 3383 is Desulfobacterium indolicum DSMZ Medium 383.2 -< for DSM 3383 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 383.1 Carrine Blank DSMZ Medium 383.1 -< for DSM 2056 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved DSM 2056 is Desulfocella sp. DSM 2056 Similar to DSMZ Medium 383, except sodium butyrate, sodium caproate, and sodium octanoate are added. DSMZ Medium 383 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved Desulfobacterium medium DSM strains: Carrine Blank A mineral-salts, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, vitamins, sodium dithionite and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 383a http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383a.pdf 383a. DESULFOTIGNUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 15.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Na-pyruvate 1.0 g Distilled water 50.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. © 2007 DSMZ GmbH - All rights reserved Desulfotignum medium An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium pyruvate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank DSM strains: DSMZ Medium 383b.3 DSMZ Medium 383b.3 -< for DSM 15286 and DSM 21156 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383b.pdf 383b. DESULFONAUTICUS MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Na-acetate 1.00 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. After inoculation pressurize vials to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. For DSM 3382 omit Na-acetate. For DSM 4206 and DSM 15269 supplement medium with 2.00 g/l Trypticase peptone and 2.00 g/l yeast extract added to the autoclaved medium from sterile anoxic stock solutions prepared under N2 gas. For DSM 15286 and DSM 21156 supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.5 – 6.7 prior to inoculation. © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 383b, except seven vitamins solution added and the pH is decreased to 6.5-6.7. DSM 15286 is Thermodesulfatator indicus DSM 15286 DSM 21156 is Thermodesulfatator atlanticus DSM 21156 Carrine Blank DSMZ Medium 383b.2 DSM 4206 is Desulfonauticus autotrophicus DSM 4206 DSM 15269 is Desulfonauticus submarinus DSMZ Medium 383b.2 -< for DSM 4206 and DSM 15269 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383b.pdf 383b. DESULFONAUTICUS MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Na-acetate 1.00 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. After inoculation pressurize vials to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. For DSM 3382 omit Na-acetate. For DSM 4206 and DSM 15269 supplement medium with 2.00 g/l Trypticase peptone and 2.00 g/l yeast extract added to the autoclaved medium from sterile anoxic stock solutions prepared under N2 gas. For DSM 15286 and DSM 21156 supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.5 – 6.7 prior to inoculation. © 2015 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 383b, except seven vitamins solution is added. The pH is decreased to 6.5-6.7. DSMZ Medium 383b.1 DSM 3382 is Desulfobacterium autotrophicum HRM2 Similar to DSMZ Medium 383b, sodium acetate is omitted. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383b.pdf 383b. DESULFONAUTICUS MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Na-acetate 1.00 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. After inoculation pressurize vials to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. For DSM 3382 omit Na-acetate. For DSM 4206 and DSM 15269 supplement medium with 2.00 g/l Trypticase peptone and 2.00 g/l yeast extract added to the autoclaved medium from sterile anoxic stock solutions prepared under N2 gas. For DSM 15286 and DSM 21156 supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.5 – 6.7 prior to inoculation. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 383b.1 -< for DSM 3382 DSMZ Medium 383b DSM strains: Carrine Blank Desulfonauticus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383b.pdf 383b. DESULFONAUTICUS MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Na-acetate 1.00 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. After inoculation pressurize vials to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. For DSM 3382 omit Na-acetate. For DSM 4206 and DSM 15269 supplement medium with 2.00 g/l Trypticase peptone and 2.00 g/l yeast extract added to the autoclaved medium from sterile anoxic stock solutions prepared under N2 gas. For DSM 15286 and DSM 21156 supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.5 – 6.7 prior to inoculation. © 2015 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium acetate, vitamins, sodium dithionite, and sodium sulfide. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide. DSMZ Medium 386.1 Carrine Blank Similar to DSMZ Medium 386, except sodium pyrosulfite is omitted and sodium thiosulfate is added. DSM 7269 is Desulfocapsa thiozymogenes DSM 7269 DSMZ Medium 386.1 -< for DSM 7269 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium386.pdf 386. DESULFOVIBRIO SULFODISMUTANS MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Na-acetate x 3 H2O 0.30 g Distilled water 10.00 ml Solution E: Vitamins solution (see medium 503) 1.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution G: Na2S2O5 (= sodium pyrosulfite) 0.95 g Distilled water 10.00 ml Adjust pH to 7.5-8.0 with NaOH. Solution A is sparged 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then distributed in anoxic Hungate-type tubes or serum vials under the same gas mixture and autoclaved. Solutions B, D and F are sparged with 100% N2 gas and autoclaved separately. Solution C is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Solutions E and G are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of the solutions B to G are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be at 7.1 - 7.4. Note: When growth has started feed culture again with same amount of pyrosulfite. After a further two days repeat feeding once more. For DSM 7269 replace solution G with 2.50 g/l Na2S2O3 x 5 H2O added to the medium after autoclaving from a sterile anoxic stock solution. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 386 Desulfovibrio sulfodismutans medium A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, trace elements, sodium bicarbonate, sodium acetate, vitamins, sodium sulfide, and sodium pyrosulfite (sodium disulfite). Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium386.pdf 386. DESULFOVIBRIO SULFODISMUTANS MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Na-acetate x 3 H2O 0.30 g Distilled water 10.00 ml Solution E: Vitamins solution (see medium 503) 1.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution G: Na2S2O5 (= sodium pyrosulfite) 0.95 g Distilled water 10.00 ml Adjust pH to 7.5-8.0 with NaOH. Solution A is sparged 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then distributed in anoxic Hungate-type tubes or serum vials under the same gas mixture and autoclaved. Solutions B, D and F are sparged with 100% N2 gas and autoclaved separately. Solution C is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Solutions E and G are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of the solutions B to G are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be at 7.1 - 7.4. Note: When growth has started feed culture again with same amount of pyrosulfite. After a further two days repeat feeding once more. For DSM 7269 replace solution G with 2.50 g/l Na2S2O3 x 5 H2O added to the medium after autoclaving from a sterile anoxic stock solution. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 388 Carrine Blank An organic-rich, liquid culture medium comprised of potassium phosphate, sodium phosphate, ammonium chloride, magnesium chloride, calcium chloride, ferrous ammonium sulfate, cobalt chloride, sodium molybdate, disodium selenite, manganese chloride, zinc sulfate, yeast extract, polypeptone, soluble starch, vitamins, resazurin, sodium carbonate, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium388.pdf 388. DICTYOGLOMUS MEDIUM KH2PO4 1.50 g Na2HPO4 x 12 H2O 4.20 g NH4Cl 0.50 g MgCl2 x 6 H2O 0.38 g CaCl2 x 2 H2O 0.06 g Fe(NH4)2(SO4)2 x 6 H2O 0.04 g CoCl2 x 6 H2O 2.90 mg Na2MoO4 x 2 H2O 2.40 mg Na2SeO3 x 5 H2O 0.17 mg MnCl2 x 4 H2O 2.00 mg ZnSO4 2.80 mg Yeast extract 2.00 g Polypeptone 2.00 g Starch, soluble 5.00 g Vitamin solution (see medium 141) 10.00 ml Resazurin 0.50 mg Na2CO3 1.00 g L-Cysteine-HCl x H2O 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except carbonate and cysteine), bring medium to the boil, then cool to room temperature under 100% N2 gas. Add and dissolve carbonate and cysteine, adjust pH to 7.2, then dispense under same gas atmosphere in culture vessels and autoclave. Adjust pH of final medium to 7.2. © 2007 DSMZ GmbH - All rights reserved Dictyoglomus medium DSMZ Medium 389 DSM strains: Thiobacillus aquaesulis medium A minerals-salts, liquid culture medium comprised of sodium phosphate, potassium phosphate, ammonium chloride, magnesium sulfate, agar, trace elements, and sodium thiosulfate. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium389.pdf 389. THIOBACILLUS AQUAESULIS MEDIUM Solution A: Na2HPO4 x 2 H2O 7.9 g KH2PO4 1.5 g Distilled water 100.0 ml Adjust to pH 7.6. Solution B: NH4Cl 0.4 g MgSO4 x 7 H2O 0.1 g Agar (for solid medium) 15.0 g Distilled water 870.0 ml Solution C: Trace elements (see medium 333) 10.0 ml Solution D: Na2S2O3 x 5 H2O 5.0 g Distilled water 20.0 ml Autoclave solutions A, B and C and filter sterilize solution D. To complete the medium combine sterile solutions when cool. © 2012 DSMZ GmbH - All rights reserved TyC solution for DSMZ Medium 391 An undefined organic chemical mixture comprised of L-tryptophan, casamino acids, and yeast extract. Prepared under an atmosphere of dinitrogen. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium391.pdf 391. METHANOCOCCUS VOLTAE MEDIUM Mineral salt solution 2xW (see below) 500.0 ml Trace element solution (see medium 141) 10.0 ml LIP-solution (see below) 50.0 ml Na-acetate 1.0 g Resazurin 0.5 mg TYC-solution (see below) 50.0 ml NaHCO3 5.0 g L-Cysteine-HCl x H2O 0.5 g Na2S x 9 H2O 0.5 g Distilled water 400.0 ml Dissolve ingredients except TYC solution, bicarbonate, cystein and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Before use add TYC solution, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to 7.2. Mineral solution 2xW: NaCl 40.00 g MgCl2 x 6 H2O 5.60 g MgSO4 x 7 H2O 0.70 g KCl 0.68 g NH4Cl 0.50 g K2HPO4 0.28 g CaCl2 x 2 H2O 0.28 g Distilled water 1000.00 ml May be stored at room temperature in the dark. LIP-solution: L-Leucine 5.0 g L-Isoleucine 10.0 g Pantothenate 0.1 g Distilled water 1000.0 ml May be stored frozen without sterilization. TYC-solution: Casamino acids 100.0 g Yeast extract 50.0 g L-Tryptophan 1.0 g Distilled water 1000.0 ml Prepare and autoclave under 100% N2 gas. For strain DSM 4254 supplement medium with 80.0 mg/l L-histidine added from a sterile anoxic stock solution sterilized by filtration. For strain DSM 4310 add 32.0 mg/l of hypoxanthine to the medium before autoclaving; supplement medium with 1.0 ml/l of a vitamins solution (see medium 503) added from a sterile anoxic stock solution sterilized by filtration. © 2010 DSMZ GmbH - All rights reserved LIP solution for DSMZ Medium 391 A defined organic chemical mixture containing L-leucine, L-sioleucine, and calcium pantothenate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium391.pdf 391. METHANOCOCCUS VOLTAE MEDIUM Mineral salt solution 2xW (see below) 500.0 ml Trace element solution (see medium 141) 10.0 ml LIP-solution (see below) 50.0 ml Na-acetate 1.0 g Resazurin 0.5 mg TYC-solution (see below) 50.0 ml NaHCO3 5.0 g L-Cysteine-HCl x H2O 0.5 g Na2S x 9 H2O 0.5 g Distilled water 400.0 ml Dissolve ingredients except TYC solution, bicarbonate, cystein and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Before use add TYC solution, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to 7.2. Mineral solution 2xW: NaCl 40.00 g MgCl2 x 6 H2O 5.60 g MgSO4 x 7 H2O 0.70 g KCl 0.68 g NH4Cl 0.50 g K2HPO4 0.28 g CaCl2 x 2 H2O 0.28 g Distilled water 1000.00 ml May be stored at room temperature in the dark. LIP-solution: L-Leucine 5.0 g L-Isoleucine 10.0 g Pantothenate 0.1 g Distilled water 1000.0 ml May be stored frozen without sterilization. TYC-solution: Casamino acids 100.0 g Yeast extract 50.0 g L-Tryptophan 1.0 g Distilled water 1000.0 ml Prepare and autoclave under 100% N2 gas. For strain DSM 4254 supplement medium with 80.0 mg/l L-histidine added from a sterile anoxic stock solution sterilized by filtration. For strain DSM 4310 add 32.0 mg/l of hypoxanthine to the medium before autoclaving; supplement medium with 1.0 ml/l of a vitamins solution (see medium 503) added from a sterile anoxic stock solution sterilized by filtration. © 2010 DSMZ GmbH - All rights reserved Carrine Blank mineral solution 2xW for DSMZ Medium 391 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium391.pdf 391. METHANOCOCCUS VOLTAE MEDIUM Mineral salt solution 2xW (see below) 500.0 ml Trace element solution (see medium 141) 10.0 ml LIP-solution (see below) 50.0 ml Na-acetate 1.0 g Resazurin 0.5 mg TYC-solution (see below) 50.0 ml NaHCO3 5.0 g L-Cysteine-HCl x H2O 0.5 g Na2S x 9 H2O 0.5 g Distilled water 400.0 ml Dissolve ingredients except TYC solution, bicarbonate, cystein and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Before use add TYC solution, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to 7.2. Mineral solution 2xW: NaCl 40.00 g MgCl2 x 6 H2O 5.60 g MgSO4 x 7 H2O 0.70 g KCl 0.68 g NH4Cl 0.50 g K2HPO4 0.28 g CaCl2 x 2 H2O 0.28 g Distilled water 1000.00 ml May be stored at room temperature in the dark. LIP-solution: L-Leucine 5.0 g L-Isoleucine 10.0 g Pantothenate 0.1 g Distilled water 1000.0 ml May be stored frozen without sterilization. TYC-solution: Casamino acids 100.0 g Yeast extract 50.0 g L-Tryptophan 1.0 g Distilled water 1000.0 ml Prepare and autoclave under 100% N2 gas. For strain DSM 4254 supplement medium with 80.0 mg/l L-histidine added from a sterile anoxic stock solution sterilized by filtration. For strain DSM 4310 add 32.0 mg/l of hypoxanthine to the medium before autoclaving; supplement medium with 1.0 ml/l of a vitamins solution (see medium 503) added from a sterile anoxic stock solution sterilized by filtration. © 2010 DSMZ GmbH - All rights reserved An inorganic salts solution comprised of sodium chloride, magnesium chloride, magnesium sulfate, potassium chloride, ammonium chloride, potassium phosphate, and calcium chloride. DSMZ Medium 391.2 Similar to DSMZ Medium 391, except hypoxanthine and seven vitamin solution are added. Carrine Blank DSM 4310 is Methanococcus voltae DSMZ Medium 391.2 -< for DSM 4310 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium391.pdf 391. METHANOCOCCUS VOLTAE MEDIUM Mineral salt solution 2xW (see below) 500.0 ml Trace element solution (see medium 141) 10.0 ml LIP-solution (see below) 50.0 ml Na-acetate 1.0 g Resazurin 0.5 mg TYC-solution (see below) 50.0 ml NaHCO3 5.0 g L-Cysteine-HCl x H2O 0.5 g Na2S x 9 H2O 0.5 g Distilled water 400.0 ml Dissolve ingredients except TYC solution, bicarbonate, cystein and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Before use add TYC solution, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to 7.2. Mineral solution 2xW: NaCl 40.00 g MgCl2 x 6 H2O 5.60 g MgSO4 x 7 H2O 0.70 g KCl 0.68 g NH4Cl 0.50 g K2HPO4 0.28 g CaCl2 x 2 H2O 0.28 g Distilled water 1000.00 ml May be stored at room temperature in the dark. LIP-solution: L-Leucine 5.0 g L-Isoleucine 10.0 g Pantothenate 0.1 g Distilled water 1000.0 ml May be stored frozen without sterilization. TYC-solution: Casamino acids 100.0 g Yeast extract 50.0 g L-Tryptophan 1.0 g Distilled water 1000.0 ml Prepare and autoclave under 100% N2 gas. For strain DSM 4254 supplement medium with 80.0 mg/l L-histidine added from a sterile anoxic stock solution sterilized by filtration. For strain DSM 4310 add 32.0 mg/l of hypoxanthine to the medium before autoclaving; supplement medium with 1.0 ml/l of a vitamins solution (see medium 503) added from a sterile anoxic stock solution sterilized by filtration. © 2010 DSMZ GmbH - All rights reserved DSMZ Medium 391.1 DSMZ Medium 391.1 -< for DSM 4254 Similar to DSMZ Medium 391, except histidine is added. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium391.pdf 391. METHANOCOCCUS VOLTAE MEDIUM Mineral salt solution 2xW (see below) 500.0 ml Trace element solution (see medium 141) 10.0 ml LIP-solution (see below) 50.0 ml Na-acetate 1.0 g Resazurin 0.5 mg TYC-solution (see below) 50.0 ml NaHCO3 5.0 g L-Cysteine-HCl x H2O 0.5 g Na2S x 9 H2O 0.5 g Distilled water 400.0 ml Dissolve ingredients except TYC solution, bicarbonate, cystein and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Before use add TYC solution, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to 7.2. Mineral solution 2xW: NaCl 40.00 g MgCl2 x 6 H2O 5.60 g MgSO4 x 7 H2O 0.70 g KCl 0.68 g NH4Cl 0.50 g K2HPO4 0.28 g CaCl2 x 2 H2O 0.28 g Distilled water 1000.00 ml May be stored at room temperature in the dark. LIP-solution: L-Leucine 5.0 g L-Isoleucine 10.0 g Pantothenate 0.1 g Distilled water 1000.0 ml May be stored frozen without sterilization. TYC-solution: Casamino acids 100.0 g Yeast extract 50.0 g L-Tryptophan 1.0 g Distilled water 1000.0 ml Prepare and autoclave under 100% N2 gas. For strain DSM 4254 supplement medium with 80.0 mg/l L-histidine added from a sterile anoxic stock solution sterilized by filtration. For strain DSM 4310 add 32.0 mg/l of hypoxanthine to the medium before autoclaving; supplement medium with 1.0 ml/l of a vitamins solution (see medium 503) added from a sterile anoxic stock solution sterilized by filtration. © 2010 DSMZ GmbH - All rights reserved DSM 4254 is Methanococcus voltae DSMZ Medium 391 DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium391.pdf 391. METHANOCOCCUS VOLTAE MEDIUM Mineral salt solution 2xW (see below) 500.0 ml Trace element solution (see medium 141) 10.0 ml LIP-solution (see below) 50.0 ml Na-acetate 1.0 g Resazurin 0.5 mg TYC-solution (see below) 50.0 ml NaHCO3 5.0 g L-Cysteine-HCl x H2O 0.5 g Na2S x 9 H2O 0.5 g Distilled water 400.0 ml Dissolve ingredients except TYC solution, bicarbonate, cystein and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Before use add TYC solution, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to 7.2. Mineral solution 2xW: NaCl 40.00 g MgCl2 x 6 H2O 5.60 g MgSO4 x 7 H2O 0.70 g KCl 0.68 g NH4Cl 0.50 g K2HPO4 0.28 g CaCl2 x 2 H2O 0.28 g Distilled water 1000.00 ml May be stored at room temperature in the dark. LIP-solution: L-Leucine 5.0 g L-Isoleucine 10.0 g Pantothenate 0.1 g Distilled water 1000.0 ml May be stored frozen without sterilization. TYC-solution: Casamino acids 100.0 g Yeast extract 50.0 g L-Tryptophan 1.0 g Distilled water 1000.0 ml Prepare and autoclave under 100% N2 gas. For strain DSM 4254 supplement medium with 80.0 mg/l L-histidine added from a sterile anoxic stock solution sterilized by filtration. For strain DSM 4310 add 32.0 mg/l of hypoxanthine to the medium before autoclaving; supplement medium with 1.0 ml/l of a vitamins solution (see medium 503) added from a sterile anoxic stock solution sterilized by filtration. © 2010 DSMZ GmbH - All rights reserved Carrine Blank Methanococcus voltae medium An organic-rich, liquid culture medium comprised of mineral solution 2xW, trace elements, LIP solution, resazurin, TYC solution, sodium bicarbonate, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen. artificial sea water for DSMZ Medium 343 An artificial seawater comprised of sodium chloride, magnesium sulfate, magnesium chloride, potassium chloride, sodium bromide, boric acid, strontium chloride, citric acid, potassium iodide, and calcium chloride. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium343.pdf 343. THERMOTOGA NEAPOLITANA MEDIUM Starch, soluble 5.0 g KH2PO4 0.5 g Trace element solution (see medium 141) 15.0 ml NiCl2 x 6 H2O 2.0 mg Artificial sea water (see below) 250.0 ml Yeast extract (Difco) 2.0 g Resazurin 0.5 mg L-Cysteine-HCl x H2O 0.5 g Na2S x 9 H2O 0.5 g Distilled water 750.0 ml Dissolve ingredients (except sulfide and cysteine) and adjust pH to 6.5. Boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere, dispense under same gas atmosphere in culture vessels (30% of volume) and autoclave. Add sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Check pH of completed medium and adjust to 6.5, if necessary. Artificial sea water: NaCl 27.70 g MgSO4 x 7 H2O 7.00 g MgCl2 x 6 H2O 5.50 g KCl 0.65 g NaBr 0.10 g H3BO3 30.00 mg SrCl2 x 6 H2O 15.00 mg Citric acid 10.00 mg KI 0.05 mg CaCl2 x 2 H2O 2.25 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 465 DSM strains: Carrine Blank A minerals-salts, liquid culture medium comprised of sodium phosphate, potassium phosphate, ammonium sulfate, magnesium chloride, calcium nitrate, and trace elements. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium465.pdf 465. MINERAL MEDIUM PH 7.25 Na2HPO4 x 2 H2O 3.50 g KH2PO4 1.00 g (NH4)2SO4 0.50 g MgCl2 x 6 H2O 0.10 g Ca(NO3)2 x 4 H2O 0.05 g Trace element solution SL-4 (see below) 1.00 ml Distilled water 1000.00 ml pH 7.25 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source. Trace element solution SL-4: EDTA 0.50 g FeSO4 x 7 H2O 0.20 g Trace element solution SL-6 (see below) 100.00 ml Distilled water 900.00 ml Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml © 2012 DSMZ GmbH - All rights reserved DSMZ Medium 383.14 DSM 15576 is Desulfatibacillum aliphaticivorans DSM 15576 DSM 16219 is Desulfatibacillum alkenivorans DSM 16219 DSMZ Medium 383.14 -< for DSM 15576 and DSM 16219 Similar to DSMZ Medium 383, except sodium caprylate (sodium octanoate) and seven vitamins solution are added. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 383.15 Similar to DSMZ Medium 383, except casamino acids are added. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 383.15 -< for DSM 17291 DSM 17291 is Thermovirga lienii DSM 17291 DSMZ Medium 383.16 Similar to DSMZ Medium 383, except sodium glutamate (monosodium glutamate) and yeast extract are added. DSM 17477 is Dethiosulfatibacter aminovorans DSM 17477 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 383.16 -< for DSM 17477 DSMZ Medium 381a DSM strains: Carrine Blank Modified Luria broth An organic-rich, liquid culture medium comprised of peptone, yeast extract, and sea salt. Modified LB http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium381a.pdf 381a. MODIFIED LB Peptone 10.0 g Yeast Extract 5.0 g Sea Salt 25.0 g Distilled water 1000.0 ml Adjust to pH 7.2 and sterilize by autoclaving. The medium may be solidified by adding 12.0 g/l agar. © 2012 DSMZ GmbH - All rights reserved DSMZ Medium 382 Minimal medium M9 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium382.pdf 382. MINERAL MEDIUM M9 for E. coli JM strains 10 x M9 salts (see below) 100.0 ml 1 M MgSO4 1.0 ml 0.1 M CaCl2 1.0 ml 1 M Thiamine-HCl x 2 H2O (filter-sterilized) 1.0 ml Glucose (20%) 10.0 ml Proline 20.0 mg Distilled water 900.0 ml The above solutions should be sterilized separately by filtration (thiamine, glucose) or autoclaving. 10 x M9 salts (per l): Na2HPO4 60.0 g KH2PO4 30.0 g NH4Cl 10.0 g NaCl 5.0 g Adjust pH to 7.4. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank An organic-rich, liquid culture medium comprised of 10 x M9 salts, magnesium sulfate, calcium chloride, thiamine hydrochloride, glucose, and proline. 10 x M9 salts solution for DSMZ Medium 382 An inorganic salts solution comprised of sodium phosphate, potassium phosphate, ammonium chloride, and sodium chloride. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium382.pdf 382. MINERAL MEDIUM M9 for E. coli JM strains 10 x M9 salts (see below) 100.0 ml 1 M MgSO4 1.0 ml 0.1 M CaCl2 1.0 ml 1 M Thiamine-HCl x 2 H2O (filter-sterilized) 1.0 ml Glucose (20%) 10.0 ml Proline 20.0 mg Distilled water 900.0 ml The above solutions should be sterilized separately by filtration (thiamine, glucose) or autoclaving. 10 x M9 salts (per l): Na2HPO4 60.0 g KH2PO4 30.0 g NH4Cl 10.0 g NaCl 5.0 g Adjust pH to 7.4. © 2007 DSMZ GmbH - All rights reserved DSMZ MEdium 381 Luria-Bertani medium Carrine Blank DSM strains: An organic-rich, solid culture medium comprised of tryptone, yeast extract, sodium chloride, and agar. LB Medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium381.pdf 381. LB (Luria-Bertani) MEDIUM Tryptone 10.0 g Yeast extract 5.0 g NaCl 10.0 g Agar 20.0 g Distilled water 1000.0 ml Adjust pH to 7.0. For DSM 26804 add 100g NaCl (10%) for 1000 ml medium. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 381.1 DSM 26804 is not in www.dsmz.de or in TaxBrower. However there is a publication that lists this as the type strain for Marinobacter piscensis (Curr Microbiol 70(4):544 2015). TaxBrowser lists this species as Marinobacter sp. Abdou3. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium381.pdf 381. LB (Luria-Bertani) MEDIUM Tryptone 10.0 g Yeast extract 5.0 g NaCl 10.0 g Agar 20.0 g Distilled water 1000.0 ml Adjust pH to 7.0. For DSM 26804 add 100g NaCl (10%) for 1000 ml medium. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 381.1 -< for DSM 26804 Similar to DSMZ Medium 381, except the concentration of sodium chloride is increased. DSMZ Medium 379 An organic-rich, liquid culture medium comprised of disodium EDTA, magnesium sulfate, calcium chloride, ammonium chloride, sodium chloride, ammonium acetate or sodium pyruvate, potassium phosphate, sodium bicarbonate, and trace elements. Chromatium tepidum medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium379.pdf 379. CHROMATIUM TEPIDUM MEDIUM Na2-EDTA 10.0 mg MgSO4 x 7 H2O 200.0 mg CaCl2 x 2 H2O 50.0 mg NH4Cl 400.0 mg NaCl 400.0 mg (NH4) acetate or Na-pyruvate 0.5 mg KH2PO4 0.5 mg NaHCO3 2.0 g Na2S x 9 H2O 1.0 g Trace element solution (see below) 1.0 ml Deionized water 1000.0 ml Prepare bicarbonate and sulfide as separate solutions. Sterilize all solutions by autoclaving. After cooling mix all three solutions, adding the sulfide last. Adjust pH to 7.0 and dispense in completely filled screw-capped tubes or bottles. Incubate at 500 - 1000 Lux light intensity. Trace element solution: Na2-EDTA 5.2 g FeCl2 x 4 H2O 1.5 g ZnCl2 70.0 mg MnCl2 x 4 H2O 100.0 mg H3BO3 6.0 mg CoCl2 x 6 H2O 190.0 mg CuCl2 x 2 H2O 2.0 mg NiCl2 x 6 H2O 25.0 mg Na2MoO4 x 2 H2O 188.0 mg VOSO4 x 2 H2O 30.0 mg Na2WO4 x 2 H2O 2.0 mg Deionized water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank trace elements solution for DSMZ Medium 379 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium379.pdf 379. CHROMATIUM TEPIDUM MEDIUM Na2-EDTA 10.0 mg MgSO4 x 7 H2O 200.0 mg CaCl2 x 2 H2O 50.0 mg NH4Cl 400.0 mg NaCl 400.0 mg (NH4) acetate or Na-pyruvate 0.5 mg KH2PO4 0.5 mg NaHCO3 2.0 g Na2S x 9 H2O 1.0 g Trace element solution (see below) 1.0 ml Deionized water 1000.0 ml Prepare bicarbonate and sulfide as separate solutions. Sterilize all solutions by autoclaving. After cooling mix all three solutions, adding the sulfide last. Adjust pH to 7.0 and dispense in completely filled screw-capped tubes or bottles. Incubate at 500 - 1000 Lux light intensity. Trace element solution: Na2-EDTA 5.2 g FeCl2 x 4 H2O 1.5 g ZnCl2 70.0 mg MnCl2 x 4 H2O 100.0 mg H3BO3 6.0 mg CoCl2 x 6 H2O 190.0 mg CuCl2 x 2 H2O 2.0 mg NiCl2 x 6 H2O 25.0 mg Na2MoO4 x 2 H2O 188.0 mg VOSO4 x 2 H2O 30.0 mg Na2WO4 x 2 H2O 2.0 mg Deionized water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved A trace elements solution comprised of disodium EDTA, ferrous chloride, zinc chloride, manganese chloride, boric acid, cobalt chloride, copper chloride, nickel chloride, sodium molybdate, vanadyl sulfate, and sodium tungstate. DSMZ Medium 380 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium380.pdf 380. MAGNETOSPIRILLUM MEDIUM KH2PO4 0.68 g NaNO3 0.12 g L(+)-Tartaric acid 0.37 g Succinic acid 0.37 g Na-acetate 0.05 g Vitamin solution (see medium 141) 10.00 ml Trace elements (see medium 141) 5.00 ml Fe(III) quinate solution (see below) 2.00 ml Agar (for semi-solid medium) 1.30 g Resazurin 0.50 mg Na-thioglycolate 0.05 g Distilled water 1000.00 ml Dissolve ingredients (except thioglycolate) in the order given and adjust pH to 6.75 with NaOH. Preparation of liquid medium: Purge medium with 100% N2 gas for 30 -45 min and dispense under the same gas atmosphere in anoxic vials to 30% of their volume. Seal vials with screw caps and gas tight rubber closures. Autoclave at 121˚C for 15 min. Before inoculation add thioglycolate from a 3% (w/v) solution, freshly prepared under 100% N2 gas and filter-sterilized. Then add sterile air (with hypodermic syringe through the rubber closure) to 1% O2 concentration in the gas phase. Preparation of semi solid medium: Supplement medium with agar, bring medium to the boil and cool under 100% N2 gas atmosphere. Dispense under same gas atmosphere aliquots of 10 ml semi-solid medium into 16 x 150 mm Hungate-type tubes. After autoclaving, tubes may be stored at room temperature. Prior to inoculation add thioglycolate from a 3% (w/v) solution, freshly prepared under 100% N2 gas and filter-sterilized. Then add sterile air (with hypodermic syringe through the rubber closure) to a concentration of 1% (v/v) in the vial. Note: Prior to inoculation media should be slightly pink in color. Strongly reduced conditions will not support growth of the organism. Incubate tubes with semi-solid medium without agitation in an upright position. During growth O2 will be consumed, resazurin decolorized and the pH increase. Feed oxygen (by adding air) and succinic acid from sterile 0.05 M solution (to maintain pH below 7). If higher densities of magnetic cell are wanted, ferric quinate also has to be fed. Ferric Quinate Solution, 0.01 M : FeCl3 x 6 H2O 0.45 g Quinic acid 0.19 g Distilled water 100.00 ml Sterilize by filtration under 100% N2 gas atmosphere. For DSM 6361 increase the amount of added O2 to a concentration of 10% (v/v) in the headspace gas atmosphere. © 2014 DSMZ GmbH - All rights reserved An organic-rich, solid culture medium comprised of potassium phosphate, sodium nitrate, tartaric acid, succinic acid, sodium acetate, vitamins, trace elements, ferric quinate solution, agar, resazurin, and sodium thioglycolate. Prepared under an atmosphere of dinitrogen and dioxygen. Carrine Blank Magnetospirillum medium DSM strains: DSMZ Medium 380.1 Similar to DSMZ Medium 380, except the concentration of dioxygen is increased. DSM 6361 is Magnetospirillum gryphiswaldense MSR-1 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium380.pdf 380. MAGNETOSPIRILLUM MEDIUM KH2PO4 0.68 g NaNO3 0.12 g L(+)-Tartaric acid 0.37 g Succinic acid 0.37 g Na-acetate 0.05 g Vitamin solution (see medium 141) 10.00 ml Trace elements (see medium 141) 5.00 ml Fe(III) quinate solution (see below) 2.00 ml Agar (for semi-solid medium) 1.30 g Resazurin 0.50 mg Na-thioglycolate 0.05 g Distilled water 1000.00 ml Dissolve ingredients (except thioglycolate) in the order given and adjust pH to 6.75 with NaOH. Preparation of liquid medium: Purge medium with 100% N2 gas for 30 -45 min and dispense under the same gas atmosphere in anoxic vials to 30% of their volume. Seal vials with screw caps and gas tight rubber closures. Autoclave at 121˚C for 15 min. Before inoculation add thioglycolate from a 3% (w/v) solution, freshly prepared under 100% N2 gas and filter-sterilized. Then add sterile air (with hypodermic syringe through the rubber closure) to 1% O2 concentration in the gas phase. Preparation of semi solid medium: Supplement medium with agar, bring medium to the boil and cool under 100% N2 gas atmosphere. Dispense under same gas atmosphere aliquots of 10 ml semi-solid medium into 16 x 150 mm Hungate-type tubes. After autoclaving, tubes may be stored at room temperature. Prior to inoculation add thioglycolate from a 3% (w/v) solution, freshly prepared under 100% N2 gas and filter-sterilized. Then add sterile air (with hypodermic syringe through the rubber closure) to a concentration of 1% (v/v) in the vial. Note: Prior to inoculation media should be slightly pink in color. Strongly reduced conditions will not support growth of the organism. Incubate tubes with semi-solid medium without agitation in an upright position. During growth O2 will be consumed, resazurin decolorized and the pH increase. Feed oxygen (by adding air) and succinic acid from sterile 0.05 M solution (to maintain pH below 7). If higher densities of magnetic cell are wanted, ferric quinate also has to be fed. Ferric Quinate Solution, 0.01 M : FeCl3 x 6 H2O 0.45 g Quinic acid 0.19 g Distilled water 100.00 ml Sterilize by filtration under 100% N2 gas atmosphere. For DSM 6361 increase the amount of added O2 to a concentration of 10% (v/v) in the headspace gas atmosphere. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 380.1 -< for DSM 6361 Carrine Blank ferric quinate solution for DSMZ Medium 380 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium380.pdf 380. MAGNETOSPIRILLUM MEDIUM KH2PO4 0.68 g NaNO3 0.12 g L(+)-Tartaric acid 0.37 g Succinic acid 0.37 g Na-acetate 0.05 g Vitamin solution (see medium 141) 10.00 ml Trace elements (see medium 141) 5.00 ml Fe(III) quinate solution (see below) 2.00 ml Agar (for semi-solid medium) 1.30 g Resazurin 0.50 mg Na-thioglycolate 0.05 g Distilled water 1000.00 ml Dissolve ingredients (except thioglycolate) in the order given and adjust pH to 6.75 with NaOH. Preparation of liquid medium: Purge medium with 100% N2 gas for 30 -45 min and dispense under the same gas atmosphere in anoxic vials to 30% of their volume. Seal vials with screw caps and gas tight rubber closures. Autoclave at 121˚C for 15 min. Before inoculation add thioglycolate from a 3% (w/v) solution, freshly prepared under 100% N2 gas and filter-sterilized. Then add sterile air (with hypodermic syringe through the rubber closure) to 1% O2 concentration in the gas phase. Preparation of semi solid medium: Supplement medium with agar, bring medium to the boil and cool under 100% N2 gas atmosphere. Dispense under same gas atmosphere aliquots of 10 ml semi-solid medium into 16 x 150 mm Hungate-type tubes. After autoclaving, tubes may be stored at room temperature. Prior to inoculation add thioglycolate from a 3% (w/v) solution, freshly prepared under 100% N2 gas and filter-sterilized. Then add sterile air (with hypodermic syringe through the rubber closure) to a concentration of 1% (v/v) in the vial. Note: Prior to inoculation media should be slightly pink in color. Strongly reduced conditions will not support growth of the organism. Incubate tubes with semi-solid medium without agitation in an upright position. During growth O2 will be consumed, resazurin decolorized and the pH increase. Feed oxygen (by adding air) and succinic acid from sterile 0.05 M solution (to maintain pH below 7). If higher densities of magnetic cell are wanted, ferric quinate also has to be fed. Ferric Quinate Solution, 0.01 M : FeCl3 x 6 H2O 0.45 g Quinic acid 0.19 g Distilled water 100.00 ml Sterilize by filtration under 100% N2 gas atmosphere. For DSM 6361 increase the amount of added O2 to a concentration of 10% (v/v) in the headspace gas atmosphere. © 2014 DSMZ GmbH - All rights reserved Carrine Blank A chemical mixture, comprised of ferric trichloride and quinic acid. Prepared under an atmosphere of dinitrogen. DSMZ Medium 399 An organic-rich, liquid culture comprised of potassium chloride, magnesium chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, yeast extract, sodium lactate, ferrous ammonium sulfate, trace elements, resazurin, sodium bicarbonate, and sodium sulfide. Prepared under an atmosphere of dinitrogen, carbon dioxide, and dihydrogen. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium399.pdf 399. ARCHAEOGLOBUS MEDIUM KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Yeast extract (Difco) 0.50 g Na-L-Lactate 1.50 g Fe(NH4)2(SO4)2 x 7 H2O 2.00 mg Trace element solution (see medium 141) 10.00 ml Resazurin 0.50 mg NaHCO3 3.00 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate and sulfide, bring to the boil, then cool quickly to room temperature while gassing with 80% N2 and 20% CO2 gas mixture, add sodium bicarbonate and adjust pH to 6.9. Distribute medium under same gas atmosphere in serum bottles (20 ml per 100 ml bottle), seal, then pressurize bottles up to 2 bar overpressure with 80% N2 and 20% CO2 gas mixture and autoclave. Before use, release excess pressure and reduce the medium with sodium sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. After inoculation, add 2 bar overpressure of 80% H2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved Archaeoglobus medium Carrine Blank DSMZ Medium 411 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium411.pdf 411. CLOSTRIDIUM ACETOBUTYLICUM MEDIUM Fresh potatoes, washed, peeled and sliced 200.0 g CaCO3 2.0 g Resazurin 0.5 mg D-Glucose 6.0 g L-Cysteine-HCl x H2O 0.5 g Distilled water 1000.0 ml Mix potatoes and CaCO3 then autoclave 30 min at 121˚C. Filter through cheese cloth, add glucose and cysteine, distribute under 100% N2 gas atmosphere in anoxic Hungatetype tubes or serum vials and autoclave 20 min at 121˚C. Note: For the reactivation of freeze-dried strains it is recommended to use the following simple milk medium: Milk Medium: Fresh milk 100.0 ml Resazurin 0.1 mg Adjust pH to 7.1. Tube and autoclave under 100% N2 gas atmosphere for 12 min at 121˚C. © 2014 DSMZ GmbH - All rights reserved Clostridium acetobutylicum medium Carrine Blank An organic-rich, liquid culture medium comprised of potato infusion, calcium carbonate, resazurin, D-glucose, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen. DSM strains: DSMZ Medium 378 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium378.pdf 378. ARTHROBACTER MEDIUM K2HPO4 1.770 g KH2PO4 0.680 g NaCl 0.140 g CaCl2 0.132 g MgSO4 x 7 H2O 0.200 g Mannitol 10.000 g Yeast extract 0.080 g FeSO4 x 7 H2O 2.500 mg H3BO3 2.900 mg CoSO4 x 7 H2O 1.200 mg CuSO4 x 5 H2O 0.100 mg MnCl2 x 4 H2O 0.090 mg Na2MoO4 x 2 H2O 2.500 mg ZnSO4 x 7 H2O 1.200 mg Distilled water 1000.000 ml Adjust pH to 7.0. Incubate under 5% O2 + 95% N2. © 2007 DSMZ GmbH - All rights reserved Arthrobacter medium DSM strains: An organic-rich, liquid culture medium comprised of potassium phosphate, sodium chloride, calcium chloride, magnesium sulfate, mannitol, yeast extract, ferrous sulfate, boric acid, cobalt sulfate, copper sulfate, manganese chloride, sodium molybdate, and zinc sulfate. Prepared under an atmosphere of dioxygen and dinitrogen. Carrine Blank DSMZ Medium 377a Carrine Blank Palaeococcus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium377a.pdf 377a. PALAEOCOCCUS MEDIUM NaCl 30.00 g MgSO4 x 7 H2O 3.50 g MgCl2 x 6 H2O 2.75 g KCl 0.33 g NaBr 0.05 g H3BO3 15.00 mg SrCl2 x 6 H2O 7.50 mg (NH4)2SO4 10.00 mg Citric acid 5.00 mg KI 0.05 mg CaCl2 x 2 H2O 0.75 g KH2PO4 0.50 g NiCl2 x 6 H2O 2.00 mg Trace elements (see medium 141) 10.00 ml Peptone 5.00 g Yeast extract 1.00 g Sulfur, powdered 30.00 g Resazurin 0.50 mg Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Prepare the medium without vitamins and sulfide, bring medium to the boil and then cool under a stream of 100% N2 gas. Add sulfide, adjust the pH to 6.5, and distribute in appropriate vessels under 100% N2 gas atmosphere, thereby taking care to transfer also the necessary amount of sulfur. Heat the vessels containing the medium in boiling water for 1 hour on 3 successive days. Do not autoclave! Prior to inoculation add vitamins from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of sodium chloride, magnesium sulfate, magnesium chloride, potassium chloride, sodium bromide, boric acid, strontium chloride, ammonium sulfate, citric acid, potassium iodide, calcium chloride, potassium phosphate, nickel chloride, trace elements, peptone, yeast extract, elemental sulfur, resazurin, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen. DSM strains: DSMZ Medium 377 DSM strains: An organic-rich, liquid culture medium comprised of sodium chloride, magnesium sulfate, magnesium chloride, potassium chloride, sodium bromide, boric acid, strontium chloride, ammonium sulfate, citric acid, potassium iodide, calcium chloride, potassium phosphate, nickel chloride, trace elements, peptone, elemental sulfur, yeast extract, resazurin, and sodium sulfide. Prepared under an atmosphere of dinitrogen. Pyrococcus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium377.pdf 377. PYROCOCCUS MEDIUM NaCl 13.85 g MgSO4 x 7 H2O 3.50 g MgCl2 x 6 H2O 2.75 g KCl 0.33 g NaBr 0.05 g H3BO3 15.00 mg SrCl2 x 6 H2O 7.50 mg (NH4)2SO4 10.00 mg Citric acid 5.00 mg KI 0.05 mg CaCl2 x 2 H2O 0.75 g KH2PO4 0.50 g NiCl2 x 6 H2O 2.00 mg Trace elements (see medium 141) 10.00 ml Peptone 5.00 g Yeast extract 1.00 g Sulfur, powdered 30.00 g Resazurin 0.50 mg Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Prepare the medium without sulfide, bring medium to the boil and then cool under a stream of 100% N2 gas. Add sulfide, adjust the pH to 6.5, and distribute in appropriate vessels under 100% N2 gas atmosphere, thereby taking care to transfer the necessary amount of sulfur. Heat the vessels containing the medium in boiling water for 1 hour on 3 successive days. Do not autoclave! © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 376 An organic-rich, liquid culture medium comprised of trypticase peptone, potassium phosphate, sodium chloride, sodium thioglycolate, elemental sulfur, and resazurin. Prepared under an atmosphere of dinitrogen. Carrine Blank AN1 medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium376.pdf 376. AN1 MEDIUM Trypticase (BBL) 10.0 g K2HPO4 1.5 g NaCl 2.5 g Na-thioglycolate 1.0 g Sulfur, powdered 8.0 g Resazurin 1.0 mg Distilled water 1000.0 ml Adjust pH to 7.3. Prepare the medium anaerobically under 100% nitrogen. Sterilize thioglycolate and sulfur separately. Sterilize the sulfur by steaming for 3 h on each of three successive days. © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 374 An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium sulfate, sodium chloride, ammonium chloride, calcium chloride, ferrous sulfate, trace elements, yeast extract, sodium acetate, sodium formate, sludge fluid, fatty acid mixture, resazurin, sodium bicarbonate, isopropyl alcohol (propan-2-ol), and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank DSM strains: Methanospirillum SK medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium374.pdf 374. METHANOSPIRILLUM SK MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see medium 119) 50.00 ml Fatty acid mixture (see medium 119) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g Isopropyl alcohol 7.50 ml Na2S x 9 H2O 0.50 g Distilled water 940.00 ml Dissolve ingredients except bicarbonate, isopropyl alcohol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add isopropyl alcohol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 7.0 – 7.2, if necessary. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 375 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium375.pdf 375. METHANOHALOBIUM MEDIUM NaCl 250.00 g NH4Cl 0.33 g KH2PO4 0.33 g KCl 0.33 g CaCl2 x 2 H2O 0.33 g MgCl2 x 6 H2O 0.33 g MgSO4 x 7 H2O 4.00 g Resazurin 0.50 mg Na2CO3 2.50 g Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 0.05 g Trimethylamine-HCl 5.00 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except carbonate, trace elements, yeast extract, trimethylamine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for at least 30 – 45 min to make it anoxic, then dispense under same gas atmosphere in anoxic Hungatetype tubes or serum vials and autoclave. Add trace elements, yeast extract, trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to 7.4 with an anoxic sterile solution of 5% w/v Na2CO3, if necessary. Note: A white precipitate that forms after autoclaving of the mineral base will dissolve again after storage at room temperature for 2 – 3 days. © 2015 DSMZ GmbH - All rights reserved Methanohalobium medium DSM strains: An organic-rich, liquid culture medium comprised of sodium chloride, ammonium chloride, potassium phosphate, potassium chloride, calcium chloride, magnesium chloride, magnesium sulfate, resazurin, sodium carbonate, trace elements, yeast extract, trimethylamine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank DSMZ Medium 373 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium373.pdf 373. METHANOGENIUM CV MEDIUM KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 0.50 mg NaHCO3 5.00 g Isopropyl alcohol 7.50 ml Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, isopropyl alcohol, vitamins and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add isopropyl alcohol and cysteine from sterile anoxic stock solutions autoclaved under 100% N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 - 7.0. © 2014 DSMZ GmbH - All rights reserved Carrine Blank Methanogenium CV medium DSM strains: An organic-rich, liquid culture medium comprised of potassium chloride, magnesium chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, sodium acetate, yeast extract, trypticase peptone, resazurin, sodium bicarbonate, isopropyl alcohol (propan-2-ol), vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSMZ Medium 372 Halobacteria medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium372.pdf 372. HALOBACTERIA MEDIUM Yeast extract 5.00 g Casamino acids 5.00 g Na-glutamate 1.00 g KCl 2.00 g Na3-citrate 3.00 g MgSO4 x 7 H2O 20.00 g NaCl 200.00 g FeCl2 x 4 H2O 36.00 mg MnCl2 x 4 H2O 0.36 mg Agar 20.00 g Add distilled water to give a final volume of 1000 ml. Adjust pH to 7.0 - 7.2 © 2007 DSMZ GmbH - All rights reserved An organic-rich, solid culture medium comprised of yeast extract, casamino acids, monosodium glutamate, potassium chloride, sodium citrate, magnesium sulfate, sodium chloride, ferrous chloride, manganese chloride, and agar. Carrine Blank DSMZ Medium 370 An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium sulfate, yeast extract, and sodium ascorbate. Prepared under an atmosphere of dinitrogen. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium370.pdf 370. HELIOBACTERIUM CHLORUM MEDIUM K2HPO4 1.0 g MgSO4 x 7 H2O 1.0 g Yeast extract 10.0 g Distilled water 1000.0 ml Adjust pH to 7.0. Boil the medium for few minutes under nitrogen gas. Add sodium ascorbate (0.5 g) and continue to bubble with nitrogen gas. Adjust the pH to 6.8. Fill immediately about 45 ml medium into 50 ml screw-capped bottles with rubber septum and screw tighten. Autoclave at 121˚C for 15 min. Incubate in low light. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Heliobacterium chlorum medium DSMZ Medium 371 An organic-rich, solid culture medium comprised of potassium phosphate, potassium chloride, ammonium chloride, magnesium sulfate, calcium sulfate, trace elements, agar, sodium chloride, disodium glutamate, yeast extract, casamino acids, and sodium carbonate. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium371.pdf 371. NATRONOBACTERIA MEDIUM KH2PO4 1.00 g KCl 1.00 g NH4Cl 1.00 g MgSO4 x 7 H2O 0.24 g CaSO4 x 2 H2O 0.17 g Trace element solution SL-10 (see medium 320) 1.00 ml Agar, if necessary 20.00 g NaCl 200.00 g Na2-glutamate 1.00 g Yeast extract 5.00 g Casamino acids 5.00 g Na2CO3 5.00 g Add distilled water to give a final volume of 1000 ml. Adjust pH to 6.5 before autoclaving! Sterilize Na2CO3 separate from medium, add after cooling. Check final pH to be 9.0 - 9.5. If agar medium is prepared, heat and dissolve agar before adding sodium chloride. © 2007 DSMZ GmbH - All rights reserved DSM strains: Natronobacteria medium DSMZ Medium 390 Carrine Blank Pyrobaculum medium A minerals-salts, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric trichloride, manganese chloride, sodium tetraborate, zinc sulfate, copper chloride, sodium molybdate, vanadyl sulfate, cobalt sulfate, resazurin, and sodium sulfide. Prepared under an atmosphere of dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium390.pdf 390. PYROBACULUM MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Resazurin 0.50 mg Na2S x 9 H2O 0.50 g Substrates (sse below) Distilled water 1000.00 ml Dissolve ingredients (except sulfide and substrates), adjust pH to 6.0 and sparge with 100% N2 gas for at least 30 min to remove dissolved oxygen. After autoclaving add substrates as listed below from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of substrates can be autoclaved with the exception of sodium thiosulfate which should be sterilized by filtration. Prior to inoculation add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH of the completed medium to the appropriate value. Substrates and further instructions are given below: For DSM 4184: Trypticase peptone 0.50 g Yeast extract 0.20 g Na2S2O3 x 5 H2O 2.00 g Adjust pH of completed medium to 6.0. For DSM 4185: Trypticase peptone 0.50 g Yeast extract 0.20 g Sulfur 20.00 g Add peptone and yeast extract from sterile, anoxic stock solutions and then aseptically distribute medium under N2 gas atmosphere in sterile, anoxic culture vessels containing already the appropriate amount of sterile sulfur powder (sterilized by steaming for 3 h on each of 3 succesive days). Adjust pH of completed medium to 6.0. For DSM 13380: Yeast extract 1.00 g Na2S2O3 x 5 H2O 1.00 g Adjust pH of completed medium to 7.0. For DSM 13514: Trypticase peptone 0.50 g Yeast extract 0.20 g Na2S2O3 x 5 H2O 2.00 g Adjust pH of completed medium to 6.8. © 2015 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 390.1 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium390.pdf 390. PYROBACULUM MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Resazurin 0.50 mg Na2S x 9 H2O 0.50 g Substrates (sse below) Distilled water 1000.00 ml Dissolve ingredients (except sulfide and substrates), adjust pH to 6.0 and sparge with 100% N2 gas for at least 30 min to remove dissolved oxygen. After autoclaving add substrates as listed below from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of substrates can be autoclaved with the exception of sodium thiosulfate which should be sterilized by filtration. Prior to inoculation add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH of the completed medium to the appropriate value. Substrates and further instructions are given below: For DSM 4184: Trypticase peptone 0.50 g Yeast extract 0.20 g Na2S2O3 x 5 H2O 2.00 g Adjust pH of completed medium to 6.0. For DSM 4185: Trypticase peptone 0.50 g Yeast extract 0.20 g Sulfur 20.00 g Add peptone and yeast extract from sterile, anoxic stock solutions and then aseptically distribute medium under N2 gas atmosphere in sterile, anoxic culture vessels containing already the appropriate amount of sterile sulfur powder (sterilized by steaming for 3 h on each of 3 succesive days). Adjust pH of completed medium to 6.0. For DSM 13380: Yeast extract 1.00 g Na2S2O3 x 5 H2O 1.00 g Adjust pH of completed medium to 7.0. For DSM 13514: Trypticase peptone 0.50 g Yeast extract 0.20 g Na2S2O3 x 5 H2O 2.00 g Adjust pH of completed medium to 6.8. © 2015 DSMZ GmbH - All rights reserved Carrine Blank DSM 4184 is Pyrobaculum islandicum DSM 4184 Similar to DSMZ Medium 390, except trypticase peptone, yeast extract, and sodium thiosulfate added. DSMZ Medium 390.1 -< for DSM 4184 DSMZ Medium 390.2 DSMZ Medium 390.2 -< for DSM 4185 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium390.pdf 390. PYROBACULUM MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Resazurin 0.50 mg Na2S x 9 H2O 0.50 g Substrates (sse below) Distilled water 1000.00 ml Dissolve ingredients (except sulfide and substrates), adjust pH to 6.0 and sparge with 100% N2 gas for at least 30 min to remove dissolved oxygen. After autoclaving add substrates as listed below from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of substrates can be autoclaved with the exception of sodium thiosulfate which should be sterilized by filtration. Prior to inoculation add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH of the completed medium to the appropriate value. Substrates and further instructions are given below: For DSM 4184: Trypticase peptone 0.50 g Yeast extract 0.20 g Na2S2O3 x 5 H2O 2.00 g Adjust pH of completed medium to 6.0. For DSM 4185: Trypticase peptone 0.50 g Yeast extract 0.20 g Sulfur 20.00 g Add peptone and yeast extract from sterile, anoxic stock solutions and then aseptically distribute medium under N2 gas atmosphere in sterile, anoxic culture vessels containing already the appropriate amount of sterile sulfur powder (sterilized by steaming for 3 h on each of 3 succesive days). Adjust pH of completed medium to 6.0. For DSM 13380: Yeast extract 1.00 g Na2S2O3 x 5 H2O 1.00 g Adjust pH of completed medium to 7.0. For DSM 13514: Trypticase peptone 0.50 g Yeast extract 0.20 g Na2S2O3 x 5 H2O 2.00 g Adjust pH of completed medium to 6.8. © 2015 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 390, except trypticase peptone, yeast extract, and elemental sulfur are added. DSM 4185 is Pyrobaculum organotrophum DSMZ Medium 390.3 DSM 13380 is Pyrobaculum oguniense TE7 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium390.pdf 390. PYROBACULUM MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Resazurin 0.50 mg Na2S x 9 H2O 0.50 g Substrates (sse below) Distilled water 1000.00 ml Dissolve ingredients (except sulfide and substrates), adjust pH to 6.0 and sparge with 100% N2 gas for at least 30 min to remove dissolved oxygen. After autoclaving add substrates as listed below from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of substrates can be autoclaved with the exception of sodium thiosulfate which should be sterilized by filtration. Prior to inoculation add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH of the completed medium to the appropriate value. Substrates and further instructions are given below: For DSM 4184: Trypticase peptone 0.50 g Yeast extract 0.20 g Na2S2O3 x 5 H2O 2.00 g Adjust pH of completed medium to 6.0. For DSM 4185: Trypticase peptone 0.50 g Yeast extract 0.20 g Sulfur 20.00 g Add peptone and yeast extract from sterile, anoxic stock solutions and then aseptically distribute medium under N2 gas atmosphere in sterile, anoxic culture vessels containing already the appropriate amount of sterile sulfur powder (sterilized by steaming for 3 h on each of 3 succesive days). Adjust pH of completed medium to 6.0. For DSM 13380: Yeast extract 1.00 g Na2S2O3 x 5 H2O 1.00 g Adjust pH of completed medium to 7.0. For DSM 13514: Trypticase peptone 0.50 g Yeast extract 0.20 g Na2S2O3 x 5 H2O 2.00 g Adjust pH of completed medium to 6.8. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 390.3 -< for DSM 13380 Similar to DSMZ Medium 390, except yeast extract and sodium thiosulfate are added. The pH is increased to 7.0. Carrine Blank DSMZ Medium 390.4 DSM 13514 is Pyrobaculum arsenaticum DSM 13514 DSMZ Medium 390.4 -< for DSM 13514 Carrine Blank Similar to DSMZ Medium 390, except trypticase peptone, yeast extract, and sodium thiosulfate are added. The pH is increased to 6.8. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium390.pdf 390. PYROBACULUM MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Resazurin 0.50 mg Na2S x 9 H2O 0.50 g Substrates (sse below) Distilled water 1000.00 ml Dissolve ingredients (except sulfide and substrates), adjust pH to 6.0 and sparge with 100% N2 gas for at least 30 min to remove dissolved oxygen. After autoclaving add substrates as listed below from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of substrates can be autoclaved with the exception of sodium thiosulfate which should be sterilized by filtration. Prior to inoculation add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH of the completed medium to the appropriate value. Substrates and further instructions are given below: For DSM 4184: Trypticase peptone 0.50 g Yeast extract 0.20 g Na2S2O3 x 5 H2O 2.00 g Adjust pH of completed medium to 6.0. For DSM 4185: Trypticase peptone 0.50 g Yeast extract 0.20 g Sulfur 20.00 g Add peptone and yeast extract from sterile, anoxic stock solutions and then aseptically distribute medium under N2 gas atmosphere in sterile, anoxic culture vessels containing already the appropriate amount of sterile sulfur powder (sterilized by steaming for 3 h on each of 3 succesive days). Adjust pH of completed medium to 6.0. For DSM 13380: Yeast extract 1.00 g Na2S2O3 x 5 H2O 1.00 g Adjust pH of completed medium to 7.0. For DSM 13514: Trypticase peptone 0.50 g Yeast extract 0.20 g Na2S2O3 x 5 H2O 2.00 g Adjust pH of completed medium to 6.8. © 2015 DSMZ GmbH - All rights reserved DSMZ Medium 392 An organic-rich, solid culture medium comprised of glucose, peptone, yeast extract, potassium phosphate, magnesium sulfate, ferric trichloride, zinc sulfate, manganese sulfate, calcium chloride, thiamine hydrochloride, biotin, folic acid, inositol, and agar. BAF medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium392.pdf 392. BAF Medium Glucose 30.0 g Peptone 2.0 g Yeast extract 0.2 g KH2PO4 0.5 g MgSO4 x 7 H2O 0.5 g FeCl3 x 6 H2O 10.0 mg ZnSO4 x 7 H2O 1.0 mg MnSO4 5.0 mg CaCl2 x 2 H2O 100.0 mg Thiamin HCl 50.0 μg Biotin 1.0 μg Folic acid 100.0 μg Inositol 50.0 μg Agar 15.0 g Distilled water 1000.0 ml pH 5.8 - 6.3. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank DSMZ Medium 394 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium394.pdf 394. CHI 1776 MEDIUM Tryptone 25.0 g Yeast extract 7.5 g 1M Tris-HCl (pH 7.5) 20.0 ml Distilled water 1000.0 ml Autoclave, cool, and then add: 1M MgCl2 5.0 ml 1% Diaminopimelic acid 10.0 ml 0.4% Thymidine 10.0 ml 20% Glucose 25.0 ml Magnesium chloride should be sterilized separately by autoclaving, and the other ingredients should be sterilized separately by filtration. © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of tryptone, yeast extract, tris, magnesium chloride, diaminopimelic acid (2,6-diaminopimelic acid', thymidine, and glucose. Carrine Blank Chi 1776 medium DSM strains: DSMZ Medium 393 DSM strains: Carrine Blank YPD medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium393.pdf 393. YPD MEDIUM Yeast extract 10.0 g Peptone 20.0 g Glucose 20.0 g Distilled water 1000.0 ml Adjust pH to 6.5. © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of yeast extract, peptone, and glucose. DSMZ Medium 395 An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, potassium chloride, calcium chloride, magnesium chloride, sodium chloride, trace elements, yeast extract, starch, resazurin, elemental sulfur, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium395.pdf 395. DESULFUROCOCCUS AMYLOLYTICUS MEDIUM NH4Cl 0.33 g KH2PO4 0.33 g KCl 0.33 g CaCl2 x 2 H2O 0.44 g MgCl2 x 6 H2O 0.70 g NaCl 0.50 g Trace elements SL-10 (see medium 320) 1.00 ml Yeast extract 0.20 g Starch 5.00 g Resazurin 0.50 mg Sulfur, powdered 10.00 g NaHCO3 0.80 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except sulfur, bicarbonate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Adjust pH to 6.2 - 6.4, dispense under 80% N2 and 20% CO2 gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add sulfur aseptically to the medium while retaining anoxic conditions. Sulfur is sterilized separately by steaming for 3 h on each of 3 successive days. Bicarbonate is added from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins (sterilized by filtration) and sulfide are added to the medium from sterile anoxic stock solutions prepared under 100% N2 gas. For DSM 16532 omit sulfur. For DSM 18924 omit bicarbonate and supplement medium after autoclaving with 2.50 g/l D-glucose. Adjust pH of completed medium to 6.5. For DSM 19380 omit sulfur and increase amount of yeast extract to 0.50 g/l. Replace starch with 2.00 g/l Trypticase peptone as substrate. © 2013 DSMZ GmbH - All rights reserved Desulfurococcus amylolyticus medium Carrine Blank DSMZ Medium 395.1 DSMZ Medium 395.1 -< for DSM 16532 Similar to DSMZ Medium 395, except elemental sulfur is omitted. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium395.pdf 395. DESULFUROCOCCUS AMYLOLYTICUS MEDIUM NH4Cl 0.33 g KH2PO4 0.33 g KCl 0.33 g CaCl2 x 2 H2O 0.44 g MgCl2 x 6 H2O 0.70 g NaCl 0.50 g Trace elements SL-10 (see medium 320) 1.00 ml Yeast extract 0.20 g Starch 5.00 g Resazurin 0.50 mg Sulfur, powdered 10.00 g NaHCO3 0.80 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except sulfur, bicarbonate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Adjust pH to 6.2 - 6.4, dispense under 80% N2 and 20% CO2 gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add sulfur aseptically to the medium while retaining anoxic conditions. Sulfur is sterilized separately by steaming for 3 h on each of 3 successive days. Bicarbonate is added from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins (sterilized by filtration) and sulfide are added to the medium from sterile anoxic stock solutions prepared under 100% N2 gas. For DSM 16532 omit sulfur. For DSM 18924 omit bicarbonate and supplement medium after autoclaving with 2.50 g/l D-glucose. Adjust pH of completed medium to 6.5. For DSM 19380 omit sulfur and increase amount of yeast extract to 0.50 g/l. Replace starch with 2.00 g/l Trypticase peptone as substrate. © 2013 DSMZ GmbH - All rights reserved DSM 16532 is Desulfurococcus fermentans DSM 16532 DSMZ Medium 395.2 Similar to DSMZ Medium 395, except bicarbonate is omitted, and D-glucose is added. The pH is increased to 6.5. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium395.pdf 395. DESULFUROCOCCUS AMYLOLYTICUS MEDIUM NH4Cl 0.33 g KH2PO4 0.33 g KCl 0.33 g CaCl2 x 2 H2O 0.44 g MgCl2 x 6 H2O 0.70 g NaCl 0.50 g Trace elements SL-10 (see medium 320) 1.00 ml Yeast extract 0.20 g Starch 5.00 g Resazurin 0.50 mg Sulfur, powdered 10.00 g NaHCO3 0.80 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except sulfur, bicarbonate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Adjust pH to 6.2 - 6.4, dispense under 80% N2 and 20% CO2 gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add sulfur aseptically to the medium while retaining anoxic conditions. Sulfur is sterilized separately by steaming for 3 h on each of 3 successive days. Bicarbonate is added from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins (sterilized by filtration) and sulfide are added to the medium from sterile anoxic stock solutions prepared under 100% N2 gas. For DSM 16532 omit sulfur. For DSM 18924 omit bicarbonate and supplement medium after autoclaving with 2.50 g/l D-glucose. Adjust pH of completed medium to 6.5. For DSM 19380 omit sulfur and increase amount of yeast extract to 0.50 g/l. Replace starch with 2.00 g/l Trypticase peptone as substrate. © 2013 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 395.2 -< for DSM 18924 DSM 18924 is Desulfurococcus kamchatkensis 1221n DSMZ Medium 395.3 DSMZ Medium 395.3 -< for DSM 19380 Similar to DSMZ Medium 395, except elemental sulfur and starch are omitted and trypticase peptone is added and the concentration of yeast extract is increased. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium395.pdf 395. DESULFUROCOCCUS AMYLOLYTICUS MEDIUM NH4Cl 0.33 g KH2PO4 0.33 g KCl 0.33 g CaCl2 x 2 H2O 0.44 g MgCl2 x 6 H2O 0.70 g NaCl 0.50 g Trace elements SL-10 (see medium 320) 1.00 ml Yeast extract 0.20 g Starch 5.00 g Resazurin 0.50 mg Sulfur, powdered 10.00 g NaHCO3 0.80 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except sulfur, bicarbonate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Adjust pH to 6.2 - 6.4, dispense under 80% N2 and 20% CO2 gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add sulfur aseptically to the medium while retaining anoxic conditions. Sulfur is sterilized separately by steaming for 3 h on each of 3 successive days. Bicarbonate is added from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins (sterilized by filtration) and sulfide are added to the medium from sterile anoxic stock solutions prepared under 100% N2 gas. For DSM 16532 omit sulfur. For DSM 18924 omit bicarbonate and supplement medium after autoclaving with 2.50 g/l D-glucose. Adjust pH of completed medium to 6.5. For DSM 19380 omit sulfur and increase amount of yeast extract to 0.50 g/l. Replace starch with 2.00 g/l Trypticase peptone as substrate. © 2013 DSMZ GmbH - All rights reserved DSM 19380 is Fervidicoccus fontis Kam940 DSMZ Medium 395a A minerals-salts, liquid culture medium comprised of ammonium chloride, potassium phosphate, potassium chloride, calcium chloride, magnesium chloride, sodium chloride, trace elements, resazurin, elemental sulfur, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium395a.pdf 395a. BROCKIA LITHOTROPHICA MEDIUM NH4Cl 0.33 g KH2PO4 0.33 g KCl 0.33 g CaCl2 x 2 H2O 0.44 g MgCl2 x 6 H2O 0.70 g NaCl 0.50 g Trace elements SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg Sulfur, powdered 10.00 g NaHCO3 0.80 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except sulfur, bicarbonate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Adjust pH to 6.2 - 6.4, dispense under 80% H2 and 20% CO2 gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add sulfur aseptically to the medium while retaining anoxic conditions. Sulfur is sterilized separately by steaming for 3 h on each of 3 successive days. Bicarbonate is added from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins (sterilized by filtration) and sulfide are added to the medium from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank DSMZ Medium 396 An organic-rich, liquid culture medium comprised of yeast extract, trypticase peptone, sodium chloride, ammonium chloride, magnesium chloride, magnesium sulfate, potassium chloride, potassium phosphate, trace elements, resazurin, L-cysteine hydrochloride, sodium bicarbonate, sodium carbonate, trimethylamine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank Methanosalsum zhilinae medium DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium396.pdf 396. METHANOSALSUM ZHILINAE MEDIUM Yeast extract (Difco) 2.00 g Trypticase (BBL) 2.00 g NaCl 40.00 g NH4Cl 1.00 g MgCl2 x 6 H2O 3.50 g MgSO4 x 7 H2O 3.00 g KCl 1.00 g K2HPO4 0.40 g Trace element solution (see medium 141) 5.00 ml Resazurin 0.50 mg L-Cysteine-HCl x H2O 0.50 g NaHCO3 3.00 g Na2CO3 2.00 g Trimethylamine-HCl 2.00 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Dissolve ingredients except cysteine, bicarbonate, carbonate, trimethylamine and sulfide, bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and dispense medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas, whereas bicarbonate and carbonate are added from sterile anoxic stock solutions prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of completed medium to 9.2 - 9.4. © 2014 DSMZ GmbH - All rights reserved DSMZ Medium 398 DSM strains: An organic-rich liquid culture medium comprised of potassium phosphate, magnesium sulfate, calcium chloride, ammonium sulfate, yeast extract, glucose, and elemental sulfur. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium398.pdf 398. THERMOPLASMA VOLCANIUM MEDIUM KH2PO4 3.00 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 0.25 g (NH4)2 SO4 0.20 g Yeast extract 1.00 g Glucose 5.00 g Sulfur (for anaerobic media only) 4.00 g Distilled water 1000.00 ml Adjust pH of the mineral base to about 2 with sulfuric acid and autoclave. Add glucose and yeast extract from filter-sterilized stock solutions. For DSM 4301 add 0.5 g/l meat extract (Merck); aerobic growth of that strain is sometimes difficult to achieve. Check pH to be 2 to 3. For anaerobic growth, the acidified mineral base containing sulfur is filled into serum bottles (20 ml per 100 ml bottle) under N2 + CO2 (80 + 20) gas mixture. Then, the bottles are sterilized by tyndallisation. Yeast extract, glucose, and meat extract (for DSM 4301 only) are added from separately sterilized stock solutions. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Thermoplasma volcanium medium DSMZ Medium 398.1 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium398.pdf 398. THERMOPLASMA VOLCANIUM MEDIUM KH2PO4 3.00 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 0.25 g (NH4)2 SO4 0.20 g Yeast extract 1.00 g Glucose 5.00 g Sulfur (for anaerobic media only) 4.00 g Distilled water 1000.00 ml Adjust pH of the mineral base to about 2 with sulfuric acid and autoclave. Add glucose and yeast extract from filter-sterilized stock solutions. For DSM 4301 add 0.5 g/l meat extract (Merck); aerobic growth of that strain is sometimes difficult to achieve. Check pH to be 2 to 3. For anaerobic growth, the acidified mineral base containing sulfur is filled into serum bottles (20 ml per 100 ml bottle) under N2 + CO2 (80 + 20) gas mixture. Then, the bottles are sterilized by tyndallisation. Yeast extract, glucose, and meat extract (for DSM 4301 only) are added from separately sterilized stock solutions. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 398.1 -< for DSM 4301 DSM 4301 is Thermoplasma volcanium Similar to DSMZ Medium 398, except meat extract is added. DSMZ Medium 398.2 DSM 4301 is Thermoplasma volcanium Similar to DSMZ Medium 398, except prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium398.pdf 398. THERMOPLASMA VOLCANIUM MEDIUM KH2PO4 3.00 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 0.25 g (NH4)2 SO4 0.20 g Yeast extract 1.00 g Glucose 5.00 g Sulfur (for anaerobic media only) 4.00 g Distilled water 1000.00 ml Adjust pH of the mineral base to about 2 with sulfuric acid and autoclave. Add glucose and yeast extract from filter-sterilized stock solutions. For DSM 4301 add 0.5 g/l meat extract (Merck); aerobic growth of that strain is sometimes difficult to achieve. Check pH to be 2 to 3. For anaerobic growth, the acidified mineral base containing sulfur is filled into serum bottles (20 ml per 100 ml bottle) under N2 + CO2 (80 + 20) gas mixture. Then, the bottles are sterilized by tyndallisation. Yeast extract, glucose, and meat extract (for DSM 4301 only) are added from separately sterilized stock solutions. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 398.2 -< for DSM 4301, anaerobic growth Carrine Blank DSMZ Medium 397 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium397.pdf 397. FERULATE MEDIUM Use medium 212 without Na2SO4 and butyrate. Add sodium ferulate to 10 mM final concentration from filter-sterilized anaerobic stock solution. © 2007 DSMZ GmbH - All rights reserved DSM strains: Similar to DSMZ Medium 212, except sodium sulfate and butyrate are omitted. Sodium ferulate is added. Carrine Blank ferulate medium DSMZ Medium 413 An organic-rich, liquid culture medium comprised of lean ground beef hydrolysate, casitone, yeast extract, potassium phosphate, resazurin, cysteine, chopped meat, hemin solution, vitamin K solution, sodium formiate (sodium formate), and sodium fumarate. BTU medium DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium413.pdf 413. BTU MEDIUM Medium 78 + Formiate/Fumarate: 78. Chopped Meat Medium Ground beef (fat free) 500.0 g Distilled water 1000.0 ml NaOH 1 N 25.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: Casitone 30.0 g Yeast extract 5.0 g K2HPO4 5.0 g Resazurin 1.0 mg Boil, cool under nitrogen atmosphere, add 0.5 g/l cysteine and adjust pH to 7.0. Dispense anoxically 7 ml medium into Hungate tubes containing meat particles (use 1 part meat particles to 4 or 5 parts fluid). Autoclave at 121˚C for 30 min. For agar slants use 15 g agar per 1000.0 ml medium. In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving: Haemin solution (see below) 10.0 ml Vitamin K1 or Vitamin K3 solution (see below) 0.2 ml Haemin solution: Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. Vitamin K1 solution: Dissolve 0.1 ml of vitamin K1/K3 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. Vitamin K3 solution: Dissolve 5 mg/ml of vitamin K3 in 10 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. Formiate/Fumarate Solution Na-formiate 6.0 g Na-fumarate 6.0 g Distilled water 100.0 ml Filter sterilize. Add 30 μl per ml of medium 78 before inoculation. © 2012 DSMZ GmbH - All rights reserved DSMZ Medium 419 An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium sulfate, magnesium sulfate, trace elements, sodium acetate, yeast extract, trypticase (peptone), sodium oxalate, resazurin, sodium carbonate, and L-cysteine hydrochloride. Prepared under an atmosphere of carbon dioxide. DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium419.pdf 419. OXALOBACTER MEDIUM K2HPO4 0.25 g KH2PO4 0.25 g (NH4)2SO4 0.50 g MgSO4 x 7 H2O 25.00 mg Trace element sol. SL-10 (see medium 320) 1.00 ml Na-acetate 0.82 g Yeast extract 1.00 g Trypticase 1.00 g Na-oxalate 5.00 g Resazurin 0.50 mg Na2CO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except carbonate and cysteine, adjust pH to 6.8, bring medium to the boil and then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate and cysteine, then equilibrate pH to 6.8. Dispense under 100% CO2 gas atmosphere in anoxic Hungate-type tubes or seruim vials and autoclave. © 2015 DSMZ GmbH - All rights reserved Oxalobacter medium DSMZ Medium 414 Carrine Blank DSM strains: An organic-rich, liquid culture medium comprised of trypticase peptone, casamino acids, yeast extract, glucose, monosodium glutamate, arginine, glycine, tryptopha, potassium phosphate, tween 80 (polysorbate 80), and cysteine hydrochloride. Acidaminococcus fermentans medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium414.pdf 414. ACIDAMINOCOCCUS FERMENTANS MEDIUM Trypticase peptone 5.0 g Casamino acids 10.0 g Yeast extract 5.0 g Glucose 5.0 g Na-glutamate 4.0 g Arginine 1.0 g Glycine 1.0 g DL-Tryptophan 0.1 g KH2PO4 2.0 g Tween 80 0.5 ml Cysteine-HCl x H2O 0.5 g Distilled water 1000.0 ml pH 7.0 - 7.2. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 421 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium421.pdf 421. CHROMATIUM SALEXIGENS MEDIUM Supplement medium 28 with NaCl (10%), MgCl2 x 6 H2O (0.3%) Na-acetate (0.05%) and sodium thiosulfate (0.05%). Incubate at 500 to 1000 Lux light intensity. © 2007 DSMZ GmbH - All rights reserved Chromatium salexigens medium DSM strains: Similar to DSMZ Medium 28, except sodium chloride, magnesium chloride, sodium acetate, and sodium thiosulfate are added. The salinity is increased to hypersaline. Carrine Blank DSMZ Medium 420 Blood agar I http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium420.pdf 420. BLOOD AGAR I Sterilize blood agar No 2 (Oxoid). After autoclaving cool to 50˚C, then add 40 ml/l of sterile horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 1. For rehydration of Helicobacter and Campylobacter spp., use medium 215 (brain heart infusion). © 2007 DSMZ GmbH - All rights reserved Carrine Blank Similar to Oxoid blood agar No. 2, except horse blood is added. DSM strains: DSMZ Medium 422 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium422.pdf 422. AMOEBOBACTER MEDIUM Supplement medium 28 with 2 mmol acetate and 1 mmol thiosulfate. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Similar to DSMZ Medium 28, except acetate and thiosulfate are added. Amoebobacter medium DSM strains: DSMZ Medium 423 Carrine Blank Xenorhabdus medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium423.pdf 423. XENORHABDUS MEDIUM Peptone 10.0 g Yeast extract 5.0 g NaCl 5.0 g Distilled water 1000.0 ml Dissolve and adjust pH to 7.2. Add 1.5% agar if required. Sterilize at 121˚C for 20 min. © 2007 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, liquid culture medium comprised of peptone, yeast extract, and sodium chloride. DSMZ Medium 425 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium425.pdf 425. OATMEAL AGAR Oat flakes 10.0 g Oatmeal 10.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0 - 7.2. © 2007 DSMZ GmbH - All rights reserved Oatmeal agar An organic-rich, solid culture medium comprised of oat flakes (rolled oats), oat meal, and agar. DSM strains: DSMZ Medium 426 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium426.pdf 426. ORGANIC MEDIUM 79 Dextrose 10.0 g Peptone 10.0 g Casein peptone 2.0 g Yeast extract 2.0 g NaCl 6.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.8. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank An organic-rich, solid culture medium comprised of dextrose (D-glucose), peptone, casein peptone (casitone), yeast extract, sodium chloride, and agar. Organic medium 79 DSMZ Medium 428 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium428.pdf 428. HETEROTROPHIC MEDIUM H3P Solution A: KH2PO4 2.300 g Na2HPO4 x 2 H2O 2.900 g Distilled water 50.000 ml Solution B: NH4Cl 1.000 g MgSO4 x 7 H2O 0.500 g CaCl2 x 2 H2O 0.010 g MnCl2 x 4 H2O 0.005 g NaVO3 x H2O 0.005 g Trace element sol. SL-6 (see medium 27) 5.000 ml Distilled water 850.000 ml Agar (if necessary) 20.000 g Solution C: Ferric ammonium citrate 0.050 g Distilled water 20.000 ml Solution D: Yeast extract 1.000 g Na-acetate 1.000 g Na2-succinate 1.000 g DL-Malate 1.000 g Distilled water 30.000 ml pH 7.0 Solution E: Na-lactate 1.000 g Na-pyruvate 1.000 g D-Mannitol 1.000 g D-Glucose 2.000 g Distilled water 50.000 ml pH 7.0 filter-sterilized Standard vitamin solution: See next page Standard vitamin solution: Riboflavin 10.000 mg Thiamine-HCl x 2 H2O 50.000 mg Nicotinic acid 50.000 mg Pyridoxine-HCl 50.000 mg Ca-pantothenate 50.000 mg Biotin 0.100 mg Folic acid 0.200 mg Vitamin B12 1.000 mg Distilled water 100.000 ml H3P is a heterotrophic medium for growth, purity checking and isolation of a broad spectrum of aerobic bacteria (Ref. 3367). Solutions A, B, C and D are autoclaved separately for 15 min at 121˚C, cooled down to 50˚C and then mixed aseptically with filter-sterilized solution E (at 50˚C) and 5.0 ml of standard vitamin solution. The final pH of this medium should be 6.8 without adjustment. © 2007 DSMZ GmbH - All rights reserved DSM strains: An organic-rich, liquid culture medium comprised of potassium phosphate, sodium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, manganese chloride, sodium metavanadate, trace elements, ferric ammonium citrate, yeast extract, sodium acetate, sodium succinate, sodium malate, sodium lactate, sodium pyruvate, mannitol, glucose, and vitamins. Heterotrophic medium H3P H3P Carrine Blank DSMZ Medium 427 DSM strains: An organic-rich, solid culture medium comprised of malt extract, glucose, asparagine, casein hydrolysate, potassium phosphate, magnesium sulfate, yeast extract, and agar. Carrine Blank Mykorrhiza medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium427.pdf 427. MYKORRHIZA MEDIUM Malt extract 8.0 g Glucose 7.0 g Asparagine 0.5 g Casein hydrolysate 1.0 g KH2PO4 0.5 g MgSO4 x 7 H2O 0.5 g Yeast extract 1.0 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 429 Similar to Columbia gar base, except horse blood is added. DSM strains: Columbia blood agar http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429.pdf 429. COLUMBIA BLOOD AGAR Columbia agar base supplemented with 4% horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below). For rehydration of Helicobacter and Campylobacter spp., use medium 215 (brain heart infusion). 545. TRYPTONE SOYA BROTH (TSB) Peptone from casein 17.0 g Peptone from soymeal 3.0 g D(+)-Glucose 2.5 g NaCl 5.0 g K2HPO4 2.5 g Distilled water 1000.0 ml Adjust pH to 7.3. The same as Caso Bouillon (Merck or Oxoid, Germany). 215. BHI MEDIUM Brain Heart Infusion (Difco) 37.0 g Distilled water 1000.0 ml 1. NUTRIENT BROTH Peptone 5.0 g Meat extract 3.0 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 429b http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429b.pdf 429b. CHOCOLATE AGAR Mix autoclaved Columbia agar base solution with 10% horse or sheep blood, keep at 80˚C for 10 min. Mix well and pour plates after cooling to 50˚C. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1. © 2007 DSMZ GmbH - All rights reserved Carrine Blank Chocolate agar DSM strains: Similar to Columbia agar base, except horse blood or sheep blood is added. DSMZ Medium 429a Similar to Columbia agar base, except activated charcoal and horse blood are added. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429a.pdf 429a. COLUMBIA BLOOD AGAR WITH CHARCOAL Add 2 g/l activated charcoal to the columbia agar base solution before autoclaving. Add 4% horse blood after cooling to 50˚C. Swirl agar during suspending. To prevent dry agar surface, spread about 1 ml of sterile medium or water over the agar plates and briefly allow to soak into the agar before inoculation. Incubate Neisseria strains in a humid microaerobic atmosphere. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1. © 2007 DSMZ GmbH - All rights reserved DSM strains: Columbia blood agar with charcoal DSMZ Medium 429d Similar to Columbia agar base, except artificial seawater, horse blood or sheep blood, glucose, iron trichloride, and cysteine are addd. The concentration of agar is increased. Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429d.pdf 429d. FRANCISELLA HALIOTICIDA MEDIUM Dissolve Columbia agar base in 70% artificial seawater and add additional 2 g agar per liter. Autoclave and mix solution with 4% horse or sheep blood after cooling to 50˚C. Add 1% glucose, 2 mM FeCl3 and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215. © 2010 DSMZ GmbH - All rights reserved Francisella halioticida medium DSMZ Medium 429c Columbia glucose cysteine agar http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429c.pdf 429c. COLUMBIA-GLUCOSE-CYSTEINE-AGAR Mix autoclaved Columbia agar base solution with 4% horse or sheep blood. After cooling to 50˚C add 1% glucose and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank Similar to Columbia agar base, except horse blood or sheep blood is added. Gluose and cysteine are also added. DSMZ Medium 430 An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, calcium chloride, magnesium chloride, sodium chlordie, sodium sulfate, sodium bicarbonate, sodium carbonate, vitamins, trace elements, sodium sulfide, and sodium acetate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium430.pdf 430. ECTOTHIORHODOSPIRA ABDELMALEKII MEDIUM KH2PO4 0.80 g NH4Cl 0.80 g CaCl2 x 2 H2O 0.05 g MgCl2 x 6 H2O 0.10 g NaCl 120.00 g Na2SO4 15.00 g NaHCO3 10.00 g Na2CO3 4 - 5.00g Vitamin solution VA * 1.00 ml Trace element solution SLA * 1.00 ml Na2S x 9 H2O 0.50 g Na-acetate 1.00 g Distilled water 1000.00 ml Adjust pH to 8.5, filter-sterilize and add appropriate amount of a sterile Na2S solution. *see medium 351 © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: Ectothiorhodospira abdelmalekii medium DSMZ Medium 432 DSM strains: Similar to DSMZ Medium 144, except the sodium chloride concentration is increased. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium432.pdf 432. HALOBACTEROIDES ACETOETHYLICUS MEDIUM Use medium no. 144 supplemented with 100 g/l of NaCl. Sterilize glucose, yeast extract and sodium sulfide separately (autoclave under nitrogen atmosphere). © 2007 DSMZ GmbH - All rights reserved Halobacteroides acetoethylicus medium Carrine Blank DSMZ Medium 431 Carrine Blank Ectothiorhodospia vacuolata medium DSM strains: An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium chloride, calcium chloride, sodium bicarbonate, sodium chloride, trace elements, vitamins, sodium sulfide, sodium thiosulfate, and sodium malate. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium431.pdf 431. ECTOTHIORHODOSPIRA VACUOLATA MEDIUM KH2PO4 1.00 g NH4Cl 0.50 g MgCl2 x 6 H2O 0.20 g CaCl2 x 2 H2O 0.10 g NaHCO3 3.00 g NaCl 30.00 g Trace element solution SLA* 1.00 ml Vitamin solution VA* 1.00 ml Na2S x 9 H2O 0.05 g Na2S2O3 x 5 H2O 0.50 g Na-malate 1.00 g Distilled water 1000.00 ml Adjust pH to 8.7, filter sterilize and add appropriate amount of Na2S solution. * see medium 351 © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 434 DSM strains: Marinococcus albus medium An organic-rich, liquid culture medium comprised of sodium chloride, magnesium chloride, magnesium sulfate, calcium chloride, potassium chloride, sodium bicarbonate, sodium bromide, proteose peptone, yeast extract, and glucose. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium434.pdf 434. MARINOCOCCUS ALBUS - MEDIUM NaCl 81.000 g MgCl2 x 6 H2O 7.000 g MgSO4 x 7 H2O 9.600 g CaCl2 0.360 g KCl 2.000 g NaHCO3 0.060 g NaBr 0.026 g Proteose Peptone No. 3 5.000 g Yeast extract 10.000 g Glucose 1.000 g Distilled water 1000.000 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 435 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium435.pdf 435. KDM-2 MEDIUM Peptone 10.0 g Yeast extract 0.5 g Cysteine-HCl x H2O 1.0 g Agar 15.0 g Calf serum 200.0 ml Distilled water 800.0 ml Adjust pH to 6.5. Dissolve peptone, yeast extract and cysteine in 800 ml of distilled water. Adjust pH to 6.5 with NaOH. Add agar and dissolve by heating. Add 2 g/l activated charcoal to the agar base solution before autoclaving. After autoclaving, cool to 45°C and add serum. © 2010 DSMZ GmbH - All rights reserved Carrine Blank KDM-2 medium DSM strains: An organic-rich, solid culture medium comprised of peptone, yeast extract, cysteine hydrochloride, agar, calf serum, and activated charcoal. DSMZ Medium 438 Bordet-Gengou medium Carrine Blank Bordet Gengou Agar base supplemented with horse blood. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium438.pdf 438. BORDET-GENGOU-MEDIUM (DIFCO) Bordet-Gengou-Agar-Base supplemented with 15% horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 1. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 436 Carrine Blank DSM strains: An organic-rich, liquid culture medium comprised of tryptone, yeast extract, glucose, cellobiose, potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, resazurin, sodium carbonate, isobutyric acid, isovaleric acid, and 2-methylbutyric acid, and cysteine hydrochloride. Prepared under an atmosphere of carbon dioxide and dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium436.pdf 436. RUMINOCOCCUS ALBUS MEDIUM Tryptone 5.0 g Yeast extract 2.0 g Glucose 3.0 g Cellobiose 2.0 g Mineral solution 1 40.0 ml Mineral solution 2 40.0 ml Resazurin 1.0 mg Distilled water 920.0 ml Boil and cool under CO2. Under a gentle stream of CO2 add: Na2CO3 4.0 g Fatty acid mixture 1.0 ml Cysteine-HCl x H2O 500.0 mg Adjust pH to 7.0, distribute under N2 into appropriate vessels and sterilize. Mineral solution 1: K2HPO4 0.6 % Mineral solution 2: KH2PO4 0.6 % (NH4)2SO4 2.0 % NaCl 1.2 % MgSO4 x 7 H2O 0.25 % CaCl2 x 7 H2O 0.16 % Fatty acid mixture: Isobutyric acid 10.0 ml Isovaleric acid 10.0 ml 2-Methylbutyric acid 10.0 ml Distilled water 70.0 ml Inoculate under CO2 and incubate at 37˚C. © 2007 DSMZ GmbH - All rights reserved Ruminococcus albus medium DSMZ Medium 439 Carrine Blank Casman Agar base supplemented with sheep blood and lysed blood solution. Casman medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium439.pdf 439. CASMAN-MEDIUM Casman-Agar-Base (Difco) supplemented with 5% sheep blood and 0.15% water-lysed sheep blood. © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 383.17 dsm 28890 is Desulfoplanes formicivorans http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 383, except sodium formate and yeast extract are added. DSMZ Medium 383.17 -< for DSM 28890 Carrine Blank amino acid solution Carrine Blank Defined organic solution comprised of a mixture of amino acids. DSMZ Medium 412 DSM strains: Acetomicrobium faecalis medium Carrine Blank The pH of this medium should be about 6.7 given the ratios of potassium phosphate. An organic-rich, liquid culture medium comprised of trypticase peptone, yeast extract, glucose, potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, sodium acetate, vitamins, trace elements, resazurin, sodium bicarbonate, and cysteine hydrochloride. Prepared under an atmosphere of dinitrogen and carbon dioxide. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium412.pdf 412. ACETOMICROBIUM FAECALIS MEDIUM Trypticase peptone 2.000 g Yeast extract 2.000 g Glucose 4.000 g K2HPO4 0.225 g KH2PO4 0.255 g (NH4)2SO4 0.255 g NaCl 0.500 g MgSO4 x 7 H2O 0.100 g CaCl2 x 2 H2O 0.070 g Na-acetate 5.000 g Vitamin solution (see medium 141) 10.000 ml Trace element solution (see medium 141) 10.000 ml Resazurin 1.000 mg NaHCO3 6.000 g Cysteine-HCl x H2O 0.500 g Distilled water 1000.000 ml Autoclave under 2 bar N2:CO2 (80:20). © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 410 DSM strains: Desulfovibrio medium An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium sulfate, sodium chloride, calcium chloride, magnesium sulfate, sodium lactate, yeast extract, resazurin, ferrous sulfate, sodium thioglycolate, and ascorbic acid. Prepared under an atmosphere of dinitrogen. Desulfovibrio medium (brackish water) Carrine Blank Brackish water Desulfovibrio medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium410.pdf 410. DESULFOVIBRIO MEDIUM (BRACKISH WATER) Solution A: K2HPO4 0.5 g NH4Cl 1.0 g Na2SO4 1.0 g NaCl 10.0 g CaCl2 x 2 H2O 0.1 g MgSO4 x 7 H2O 2.0 g Na-DL-lactate 2.0 g Yeast extract 1.0 g Resazurin 1.0 mg Distilled water 980.0 ml Solution B: FeSO4 x 7 H2O 0.5 g Distilled water 10.0 ml Solution C: Na-thioglycolate 0.1 g Ascorbic acid 0.1 g Distilled water 10.0 ml Bring solution A to the boil, then cool to room temperature while gassing with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121˚C. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 408 An organic-rich, liquid culture medium comprised of peptone, yeast extract, casamino acids, L-glutamic acid, ammonium chloride, potassium phosphate, sodium chloride, trace elements, resazurin, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium408.pdf 408. PY-GLUTAMATE MEDIUM Peptone (Difco) 10.0 g Yeast extract 3.0 g Casamino acids 10.0 g L-Glutamic acid 20.0 g NH4Cl 2.0 g K2HPO4 x 3 H2O 2.0 g NaCl 25.0 g Trace elements solution (sse medium 141) 10.0 ml Resazurin 0.5 mg L-Cysteine-HCl x H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except cysteine), bring medium to the boil, then cool to room temperature under 100% N2 gas. Add and dissolve cysteine, adjust pH to 7.0 and dispense medium under 100% N2 gas atmosphere in culture vessels and autoclave. © 2014 DSMZ GmbH - All rights reserved Carrine Blank PY-glutamate medium DSM strains: DSMZ Medium 407 Syntrophomonas SD2 medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium407.pdf 407. SYNTROPHOMONAS SD2 MEDIUM Solution A: Mineral solution (see medium 212) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Na2SO4 2.80 g Resazurin 1.00 mg Distilled water 790.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: Na-stearate 0.61 g Distilled water 20.00 ml Dissolve stearate by heating in water bath. Solution E: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution F: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 min. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D, E and F are autoclaved under 100% N2 gas. To complete the medium, appropriate amounts of solutions B, C, D, E, and F are added to solution A. Adjust pH of final medium to 7.2. Note: Use 25 - 50% inoculum. Growth can be followed by the clearing of stearate. Maintain cultures at 25˚C. © 2014 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of mineral solution, trace elements, rumen fluid, trypticase (peptone), sodium sulfate, resazurin, sodium bicarbonate, vitamins, sodium stearate, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Carrine Blank DSM strains: DSMZ Medium 405 SP2 medium An organic-rich, solid culture medium comprised of casitone, yeast extract, sodium acette, agar, and natural seawater or artificial seawater. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium405.pdf 405. SP2 MEDIUM Casitone (Difco) 1.00 g Yeast extract (Difco) 0.20 g Na-acetate 0.02 g Agar (Difco), if necessary 15.00 g Sea water-natural or artificial 1000.00 ml Adjust pH to 7.2. If natural sea water is used, the pH need not be adjusted. Artificial sea water: NaCl 24.70 g KCl 0.70 g MgSO4 x 7 H2O 6.30 g MgCl2 x 6 H2O 4.60 g CaCl2 x 2 H2O 1.30 g NaHCO3 0.20 g Distilled water 1000.00 ml To prevent precipitation, CaCl2 and NaHCO3 are autoclaved each as separate stock solutions in 10 ml distilled water. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: DSMZ Medium 404 DSM strains: Carrine Blank Methanosphaera cuniculi medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium404.pdf 404. METHANOSPHAERA CUNICULI MEDIUM Mineral solution 1 (see below) 40.00 ml Mineral solution 2 (see below) 40.00 ml Fe(NH4)2(SO4)2 x 7 H2O 2.00 mg NiCl2 x 6 H2O 0.20 mg Trace elements (see medium 141) 10.00 ml Na-acetate x 3 H2O 3.00 g Yeast extract 1.00 g Trypticase 1.00 g Resazurin 0.50 mg NaHCO3 3.00 g Methanol 5.00 ml Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 900.00 ml Dissolve ingredients (except bicarbonate, methanol, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Add and dissolve bicarbonate and adjust pH to 7.0, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add methanol, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Vitamins are sterilized by filtration. Adjust pH of final medium to 7.0 – 7.2. After inoculation, pressurize culture bottles with sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Mineral solution 1: K2HPO4 6.00 g Distilled water 1000.00 ml Mineral solution 2: KH2PO4 6.00 g (NH4)2SO4 6.00 g NaCl 12.00 g MgSO4 x 7 H2O 2.60 g CaCl2 x 2 H2O 0.16 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of mineral solutions, ferrous ammonium sulfate, nickel chloride, trace elements, sodium acetate, yeast extract, trypticase (peptone), resazurin, sodium bicarbonate, methanol, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen. DSMZ Medium 403 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium403.pdf 403. BSK-MEDIUM Solution A: CMRL 1066/10 x Glutamin (GIBCO 042-1545) 100.0 ml Proteose peptone No 2 (DIFCO 0121-01-3) 5.0 g Proteose tryptone (DIFCO 0123-01) 1.0 g Proteose yeastolate (TC) (DIFCO 5577-15) 1.0 g HEPES buffer (acid) (SIGMA H3375) 6.0 g Na-citrate 0.7 g Glucose (α-, D+) 3.0 g Na-pyruvate 0.8 g N-Acetylglucosamine 0.4 g MgCl2 x 6 H2O 0.3 g L-Glutamine 0.1 g Aqua bidest. filter-sterilized 900.0 ml Stir slowly at 4 - 10˚C for 3 hours, adjust pH to 7.6 with 5 M NaOH. Filter sterilize. Solution B: Dissolve 14.0 g gelatine in 200 ml bidest. water, autoclave 15 minutes at 115˚C. Solution C: Dissolve 50.05 g bovine serum albumine, fract. V (important: Sigma No A9647) in 143 ml bidest. water, or use BSA-solution Sigma A7409. Solution D: Rabbit serum, inactivated (GIBCO 037-06120M) 86.0 ml Combine the warm (37˚C) solutions A, B, C, D, and filter-sterilize the warm medium. Solution B can also be added after filtration. Alternatively, buy supplemented or un-supplemented BSK-H medium (Fa. Bio&SELL or Sigma) and supplement the medium according to manufacturer’s advice. Sterilize the mixed and warm (37˚C) medium by filtration. © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of CMRL 1066 medium with glutamine, Proteose Peptone No. 2, Proteose Tryptone (Bacto Tryptone), Proteose Yeastolate (Bacto TC Yeastolate, or Yeastolate), HEPES, sodium citrate, D-glucose, sodium pyruvate, N-acetylglucosamine, magnesium chloride, L-glutamine, gelatin, bovine serum albumin, and rabbit serum. Carrine Blank DSM strains: BSK medium DSMZ Medium 402 Alicyclobacillus medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium402.pdf 402. ALICYCLOBACILLUS MEDIUM Solution A: CaCl2 x 2 H2O 0.25 g MgSO4 x 7 H2O 0.50 g (NH4)2 SO4 0.20 g Yeast extract 2.00 g Glucose 5.00 g KH2PO4 3.00 g Distilled water (for liquid medium) 1000.00 ml Distilled water (for solid medium) 500.00 ml Adjust pH to 4.0 Solution B: Trace element sol. SL-6 (see medium 27) 1.00 ml Solution C: Agar 15.00 g Distilled water 500.00 ml Sterilize separately. For liquid medium combine solution A (with 1000.0 ml distilled water) and solution B. For solid medium combine solution A (with 500 ml distilled water), solution B and solution C. For strains of A. cycloheptanicus add 5 g/l of yeast extract instead of 2 g/l. © 2007 DSMZ GmbH - All rights reserved An organic-rich, solid culture medium comprised of calcium chloride, magnesium sulfate, ammonium sulfate, yeast extract, glucose, potassium phosphate, trace elements, and agar. DSM strains: Oxoid Blood Agar Base No. 2 Carrine Blank http://www.oxoid.com/uk/blue/prod_detail/prod_detail.asp?pr=CM0271&org=153&c=uk&lang=EN BLOOD AGAR BASE NO.2 Code: CM0271 An improved Blood Agar Base possessing enhanced nutritional properties suitable for the cultivation of fastidious pathogens and other micro-organisms. Typical Formula* gm/litre Proteose peptone 15.0 Liver digest 2.5 Yeast extract 5.0 Sodium chloride 5.0 Agar 12.0 pH 7.4 ± 0.2 @ 25°C * Adjusted as required to meet performance standards Directions Suspend 40g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and add 7% sterile blood. Mix with gentle rotation and pour into sterile dishes or other containers. Description Oxoid Blood Agar Base No.2 was developed to meet the demand for an especially nutritious blood agar base which would permit the maximum recovery of delicate organisms without interfering with their haemolytic reactions. In comparison with fresh digest agar, Blood Agar Base No.2 may be shown to have equal or superior growth promoting properties and chromogenic bacteria grown on the Oxoid medium show enhanced pigment formation. Comparison with many other blood agars has shown that with Oxoid Blood Agar Base No.2 growth of many bacteria - especially the fastidious streptococci and pneumococci - is considerably improved, as shown by luxuriant and early colonial development. Oxoid Blood Agar Base No.2 is specified by the American Food and Drug Administration for the preparation of sheep blood agar1. Phillips2, described an improved medium for sporulation of Clostridium perfringens based on Blood Agar Base No.2 to which are added lysed horse blood, bile, sodium bicarbonate and quinoline. The medium induced significant sporulation in all of 100 strains of Clostridium perfringens isolated from human faeces. An organic-rich, solid culture medium comprised of proteose peptone, liver digest, yeast extract, sodium chloride, and agar. lean ground beef hydrolysate A beef extract prepared from lean ground beef (chopped lean ground beef) and sodium hydroxide that has been treated using heating and filtration. Carrine Blank rabbit serum A serum medium ingredient, derived from the serum (coagulated blood) from a rabbit (Oryctolagus cuniculus). Carrine Blank Proteose Peptone No. 2 Carrine Blank A Proteose Peptone made from only porcine (Sus scrofa) protein (muscle tissue). From: https://m.bdbiosciences.com/ca/cell-culture/media-supplements/media-supplements/ao-animal-origin/porcine/proteose-peptone-no-2/p/212120 BD Bacto™ Proteose Peptone No. 2 is an animal-origin, enzymatic digest of protein. Similar to BD Bacto™ Proteose Peptone, this supplement provides relatively high proteose content and varying concentrations of nutrients, vitamins, minerals, peptides of varying sizes, buffering capabilities, and nitrogen content. However, BD Bacto™ Proteose Peptone No. 2 contains only porcine origin protein. Customers have successfully used this supplement to produce toxin-based vaccines, such as Bordetella pertusis for human health and other animal health vaccines. mineral solution 1 for DSMZ Medium 404 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium404.pdf 404. METHANOSPHAERA CUNICULI MEDIUM Mineral solution 1 (see below) 40.00 ml Mineral solution 2 (see below) 40.00 ml Fe(NH4)2(SO4)2 x 7 H2O 2.00 mg NiCl2 x 6 H2O 0.20 mg Trace elements (see medium 141) 10.00 ml Na-acetate x 3 H2O 3.00 g Yeast extract 1.00 g Trypticase 1.00 g Resazurin 0.50 mg NaHCO3 3.00 g Methanol 5.00 ml Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 900.00 ml Dissolve ingredients (except bicarbonate, methanol, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Add and dissolve bicarbonate and adjust pH to 7.0, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add methanol, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Vitamins are sterilized by filtration. Adjust pH of final medium to 7.0 – 7.2. After inoculation, pressurize culture bottles with sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Mineral solution 1: K2HPO4 6.00 g Distilled water 1000.00 ml Mineral solution 2: KH2PO4 6.00 g (NH4)2SO4 6.00 g NaCl 12.00 g MgSO4 x 7 H2O 2.60 g CaCl2 x 2 H2O 0.16 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved An inorganic salts solution comprised of potassium phosphate. mineral solution 2 for DSMZ Medium 404 Carrine Blank An inorganic salts solution comprised of potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, and calcium chloride. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium404.pdf 404. METHANOSPHAERA CUNICULI MEDIUM Mineral solution 1 (see below) 40.00 ml Mineral solution 2 (see below) 40.00 ml Fe(NH4)2(SO4)2 x 7 H2O 2.00 mg NiCl2 x 6 H2O 0.20 mg Trace elements (see medium 141) 10.00 ml Na-acetate x 3 H2O 3.00 g Yeast extract 1.00 g Trypticase 1.00 g Resazurin 0.50 mg NaHCO3 3.00 g Methanol 5.00 ml Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 900.00 ml Dissolve ingredients (except bicarbonate, methanol, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Add and dissolve bicarbonate and adjust pH to 7.0, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add methanol, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Vitamins are sterilized by filtration. Adjust pH of final medium to 7.0 – 7.2. After inoculation, pressurize culture bottles with sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Mineral solution 1: K2HPO4 6.00 g Distilled water 1000.00 ml Mineral solution 2: KH2PO4 6.00 g (NH4)2SO4 6.00 g NaCl 12.00 g MgSO4 x 7 H2O 2.60 g CaCl2 x 2 H2O 0.16 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved oat meal Wikipedia:Oatmeal Oatmeal is oat groats (i.e. grains) that have been ground, steel-cut, crushed, or rolled. Ground oats are also called 'white oats'. The term 'oatmeal' is also used in the U.S. and parts of Canada to mean oat porridge. Carrine Blank Rolled oats that have been ground or crushed or steel-cut. standard vitamin solution for DSMZ Medium 428 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium428.pdf 428. HETEROTROPHIC MEDIUM H3P Solution A: KH2PO4 2.300 g Na2HPO4 x 2 H2O 2.900 g Distilled water 50.000 ml Solution B: NH4Cl 1.000 g MgSO4 x 7 H2O 0.500 g CaCl2 x 2 H2O 0.010 g MnCl2 x 4 H2O 0.005 g NaVO3 x H2O 0.005 g Trace element sol. SL-6 (see medium 27) 5.000 ml Distilled water 850.000 ml Agar (if necessary) 20.000 g Solution C: Ferric ammonium citrate 0.050 g Distilled water 20.000 ml Solution D: Yeast extract 1.000 g Na-acetate 1.000 g Na2-succinate 1.000 g DL-Malate 1.000 g Distilled water 30.000 ml pH 7.0 Solution E: Na-lactate 1.000 g Na-pyruvate 1.000 g D-Mannitol 1.000 g D-Glucose 2.000 g Distilled water 50.000 ml pH 7.0 filter-sterilized Standard vitamin solution: See next page Standard vitamin solution: Riboflavin 10.000 mg Thiamine-HCl x 2 H2O 50.000 mg Nicotinic acid 50.000 mg Pyridoxine-HCl 50.000 mg Ca-pantothenate 50.000 mg Biotin 0.100 mg Folic acid 0.200 mg Vitamin B12 1.000 mg Distilled water 100.000 ml H3P is a heterotrophic medium for growth, purity checking and isolation of a broad spectrum of aerobic bacteria (Ref. 3367). Solutions A, B, C and D are autoclaved separately for 15 min at 121˚C, cooled down to 50˚C and then mixed aseptically with filter-sterilized solution E (at 50˚C) and 5.0 ml of standard vitamin solution. The final pH of this medium should be 6.8 without adjustment. © 2007 DSMZ GmbH - All rights reserved A vitamin solution comprised of riboflavin, thiamine hydrochloride, nicotinic acid, pyridoxine hydrochloride, calcium pantothenate, biotin, folic acid, and vitamin B12 (cobalamin). Carrine Blank Bordet Gengou Agar Base Carrine Blank From: Bordet Gengou Agar Base (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Bordet Gengou Agar Base, with the addition of glycerol and sterile blood, is used in qualitative procedures for the detection and isolation of Bordetella pertussis from clinical specimens. Summary and Explanation Bordet Gengou Blood Agar is used in clinical laboratories as a method of diagnosing whooping cough. Bordetella pertussis, the etiologic agent of this disease, may be isolated from aspirated bronchial or nasopharyngeal secretions, perinasal swabs or, perhaps with greater difficulty due to the diversity of flora, from throat swabs.1 Bordet and Gengou introduced the medium in 1906 as a method of maintaining stock cultures.2 In 1934, Kendrick and Eldering replaced the 50% human or rabbit blood recommended in the original formulation with 15% sheep blood to make the medium more practical for laboratories to produce for routine clinical procedures.3 Principles of the Procedure Bordet Gengou Blood Agar contains potato infusion and glycerol to supply the nutrients necessary to support the growth of B. pertussis. Defibrinated animal blood supplies additional nutrients and enables the detection of hemolytic reactions, which aid in the identification of B. pertussis. Formula Difco™ Bordet Gengou Agar Base Approximate Formula* Per Liter Potato, Infusion from 125 g......................................... 4.5 g Sodium Chloride.......................................................... 5.5 g Agar.......................................................................... 20.0 g *Adjusted and/or supplemented as required to meet performance criteria. Directions for Preparation from Dehydrated Product 1. Suspend 30 g of the powder in 1 L of purified water containing 10 g of glycerol. Mix thoroughly. 2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3. Autoclave at 121°C for 15 minutes. 4. Aseptically add 15% sterile, defibrinated blood to the medium at 45-50°C. Mix well. 5. Test samples of the finished product for performance using stable, typical control cultures. An organic-rich, solid culture medium comprised of potato infusion, sodium chloride, agar, glycerol, and defibrinated blood. water-lysed sheep blood Carrine Blank Lysed blood solution where the blood is sheep's (Ovis aries) blood. corn starch Starch isolated from corn. Carrine Blank lysed blood solution Carrine Blank Prepared by lysing blood by adding water. partially lysed blood Casman Agar Base From: Casman Agar Base (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Casman Agar Base is used for the cultivation of fastidious pathogenic organisms, such as Haemophilus influenzae and Neisseria gonorrhoeae, from clinical specimens. Summary and Explanation Members of the genus Haemophilus are fastidious microorganisms that require the addition of X and/or V growth factors for in vitro cultivation.1 Neisseria are also fastidious microorganisms with complex growth requirements.2 In 1947, Casman described a blood-enriched medium prepared without an infusion of fresh meat for cultivation of Haemophilus and gonococci.1 The medium was developed to replace previous formulations that required time-consuming preparations of fresh and heated blood and fresh meat infusion to supply the nutrients necessary for the growth of these fastidious organisms.2,3 Casman found that nicotinamide interfered with the activity of an enzyme in blood that inactivates V factor (NAD). Using unheated human blood, he found that amount of nicotinamide required for good growth of H. influenzae was inhibitory to gonococci.2 Therefore, he reduced the nicotinamide to a level that allowed good growth of gonococci. Formula BBL™ Casman Agar Base Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 11.5 g Peptic Digest of Animal Tissue...................................... 5.0 g Yeast Extract................................................................ 3.5 g Beef Extract.................................................................. 3.0 g Nicotinamide................................................................ 0.05 g p-Aminobenzoic Acid................................................... 0.05 g Dextrose...................................................................... 0.5 g Cornstarch................................................................... 1.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 13.5 g *Adjusted and/or supplemented as required to meet performance criteria. Directions for Preparation from Dehydrated Product 1. Suspend 43 g of powder in 1 L of purified water. Mix thoroughly. 2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3. Autoclave at 121°C for 15 minutes. 4. Cool to 45-50°C and add 5% sterile blood and 0.15% blood solution, made by lysing 1 part of blood with 3 parts of water. Alternatively, add 5% partially lysed blood. 5. Test samples of the finished product for performance using stable, typical control cultures. Carrine Blank An organic-rich, solid culture medium comprised of pancreatic digest of casein (casitone), peptic digest of animal tissue, yeast extract (peptone), beef extract, nicotinamide, p-aminobenzoic acid, dextrose (D-glucose), cornstarch, sodium chloride, agar, and lysed blood solution. DSMZ Medium 465a Similar to DSMZ Medium 465, except 2-hydroxybiphenyl (biphenyl-2-ol) is added. mineral medium with 2-hydroxybiphenyl Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium465a.pdf 465a. MINERAL MEDIUM WITH 2-HYDROXYBIPHENYL Prepare medium 465. Dissolve 2-hydroxybiphenyl in ethanol (50 g/l) and filter sterilize. Pipette a volume of this stock solution into a sterile culture vessel so that the final concentration of 2-hydroxybiphenyl will be 0.5 g/l medium. Let the ethanol evaporate under sterile conditions. Add medium 465. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 464 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium464.pdf 464. PLATE COUNT AGAR Tryptone 5.0 g Yeast extract 2.5 g Dextrose 1.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved plate count agar An organic-rich, solid culture medium comprised of tryptone, yeast extract, dextrose (D-glucose), and agar. DSM strains: Carrine Blank DSMZ Medium 463a EDTA-medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium463a.pdf 463a. EDTA-MEDIUM Na2HPO4 x 2 H2O 0.41 g KH2PO4 0.26 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 0.40 g Trace element solution 1.00 ml Vitamin solution 1.00 ml Ethylenediamintetraacetic acid (EDTA), di- or trisodium salt 0.50 g Distilled water 1000.00 ml pH 8.0 Trace element solution: FeCl2 x 4 H2O 1.50 g ZnCl2 68.00 mg MnCl2 x 4 H2O 100.00 mg H3BO3 62.00 mg CoCl2 x 6 H2O 120.00 mg CuCl2 x 2 H2O 17.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 24.00 mg HCl, 0.05 molar 1000.00 ml Vitamin solution: Vitamin B12 50.00 mg Pantothenic acid 50.00 mg Riboflavin 50.00 mg Pyridoxamine-HCl 10.00 mg Biotin 20.00 mg Folic acid 20.00 mg Nicotinic acid 25.00 mg Nicotine amide 25.00 mg α-lipoic acid 50.00 mg p-aminobenzoic acid 50.00 mg Thiamine-HCl x 2 H2O 50.00 mg Distilled water 1000.00 ml Stir for some hours, filter sterilize the solution. © 2010 DSMZ GmbH - All rights reserved A minerals-salts, liquid culture medium comprised of sodium phosphate, potassium phosphate, magnesium sulfate, calcium chloride, trace elements, vitamins, and EDTA. DSM strains: DSMZ http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSM strains: Carrine Blank xxxx DSMZ DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf xxxx DSMZ Medium 462a http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium462a.pdf 462a. PLATES WITH FLUORANTHENE Pour plates of medium 462. Dissolve 2 mg/ml fluoranthene in acetone and spread 1 ml of this solution on the surface of every pre-dried plate. Allow the acetone to evaporate under sterile conditions. Inoculate as usual. © 2007 DSMZ GmbH - All rights reserved DSM strains: plates with fluoranthene Carrine Blank Similar to DSMZ Medium 462, except fluoranthene is added. DSMZ Medium 463 A minerals-salts, liquid medium comprised of sodium phosphate, potassium phosphate, magnesium sulfate, calcium chloride, trace elements, vitamins, and sodium nitrilotriacetate. DSM strains: nitrilotriacetate medium Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium463.pdf 463. NITRILOTRIACETATE MEDIUM Na2HPO4 x 2 H2O 0.41 g KH2PO4 0.26 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 0.20 g Trace element solution 1.00 ml Vitamin solution 1.00 ml Nitrilotriacetate 1.00 g Distilled water 1000.00 ml pH 6.5 Trace element solution: FeCl2 x 4 H2O 1.50 g ZnCl2 68.00 mg MnCl2 x 4 H2O 100.00 mg H3BO3 62.00 mg CoCl2 x 6 H2O 120.00 mg CuCl2 x 2 H2O 17.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 24.00 mg HCl, 0.05 molar 1000.00 ml Vitamin solution: Vitamin B12 50.00 mg Pantothenic acid 50.00 mg Riboflavin 50.00 mg Pyridoxamine-HCl 10.00 mg Biotin 20.00 mg Folic acid 20.00 mg Nicotinic acid 25.00 mg Nicotine amide 25.00 mg α-lipoic acid 50.00 mg p-aminobenzoic acid 50.00 mg Thiamine-HCl x 2 H2O 50.00 mg Distilled water 1000.00 ml Stir for some hours, filter sterilize the solution. © 2008 DSMZ GmbH - All rights reserved DSMZ Carrine Blank xxxx DSM strains: trace elements solution for DSMZ Medium 463 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium463.pdf 463. NITRILOTRIACETATE MEDIUM Na2HPO4 x 2 H2O 0.41 g KH2PO4 0.26 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 0.20 g Trace element solution 1.00 ml Vitamin solution 1.00 ml Nitrilotriacetate 1.00 g Distilled water 1000.00 ml pH 6.5 Trace element solution: FeCl2 x 4 H2O 1.50 g ZnCl2 68.00 mg MnCl2 x 4 H2O 100.00 mg H3BO3 62.00 mg CoCl2 x 6 H2O 120.00 mg CuCl2 x 2 H2O 17.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 24.00 mg HCl, 0.05 molar 1000.00 ml Vitamin solution: Vitamin B12 50.00 mg Pantothenic acid 50.00 mg Riboflavin 50.00 mg Pyridoxamine-HCl 10.00 mg Biotin 20.00 mg Folic acid 20.00 mg Nicotinic acid 25.00 mg Nicotine amide 25.00 mg α-lipoic acid 50.00 mg p-aminobenzoic acid 50.00 mg Thiamine-HCl x 2 H2O 50.00 mg Distilled water 1000.00 ml Stir for some hours, filter sterilize the solution. © 2008 DSMZ GmbH - All rights reserved A trace elements solution comprised of ferrous chloride, zinc chloride, manganese chloride, boric acid, cobalt chloride, copper chloride, nickel chloride, sodium molybdate, and hydrogen chloride. Carrine Blank DSMZ Medium 461c DSM strains: Carrine Blank mineral medium with o-nitrophenol http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium461c.pdf 461c. MINERAL MEDIUM WITH O-NITROPHENOL Dissolve 0.5 g o-nitrophenol per liter phosphate buffer (50 mM, pH 7.5) and sterilize by filtration. Prepare and autoclave medium 461 (vitamins not necessary). Add 200 ml nitrophenol solution to 800 ml medium 461. © 2007 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 461, except o-nitrophenol (2-nitrophenol) is added. DSMZ Medium 462 A minerals-salts, liquid culture medium comprised of sodium phosphate, potassium phosphate, ammonium sulfate, magnesium sulfate, calcium chloride, trace elements, and vitamins. Brunner mineral medium with vitamins mineral medium with vitamins http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium462.pdf 462. MINERAL MEDIUM (BRUNNER) WITH VITAMINS Na2HPO4 2.44 g KH2PO4 1.52 g (NH4)2SO4 0.50 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.05 g Trace element sol. SL-4 (see below) 10.00 ml Vitamin solution (Schlegel, see below) 2.50 ml Distilled water 1000.00 ml Adjust pH to 6.9. Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 462 with the appropriate carbon source. Trace element solution SL-4: EDTA 0.50 g FeSO4 x 7 H2O 0.20 g Trace element solution SL-6 (see below) 100.00 ml Distilled water 900.00 ml Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml Vitamin solution: p-Aminobenzoate 1.0 mg Biotin 0.2 mg Nicotinic acid 2.0 mg Thiamine-HCl x 2 H2O 1.0 mg Ca-pantothenate 0.5 mg Pyridoxamine 5.0 mg Vitamin B12 2.0 mg Distilled water 100.0 ml © 2010 DSMZ GmbH - All rights reserved DSM strains: mineral medium (Brunner) with vitamins Carrine Blank vitamin solution for DSMZ Medium 462 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium462.pdf 462. MINERAL MEDIUM (BRUNNER) WITH VITAMINS Na2HPO4 2.44 g KH2PO4 1.52 g (NH4)2SO4 0.50 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.05 g Trace element sol. SL-4 (see below) 10.00 ml Vitamin solution (Schlegel, see below) 2.50 ml Distilled water 1000.00 ml Adjust pH to 6.9. Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 462 with the appropriate carbon source. Trace element solution SL-4: EDTA 0.50 g FeSO4 x 7 H2O 0.20 g Trace element solution SL-6 (see below) 100.00 ml Distilled water 900.00 ml Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml Vitamin solution: p-Aminobenzoate 1.0 mg Biotin 0.2 mg Nicotinic acid 2.0 mg Thiamine-HCl x 2 H2O 1.0 mg Ca-pantothenate 0.5 mg Pyridoxamine 5.0 mg Vitamin B12 2.0 mg Distilled water 100.0 ml © 2010 DSMZ GmbH - All rights reserved A vitamin solution comprised of p-aminobenzoate (4-aminobenzoate), biotin, nicotinic acid, thiamine hydrochloride, calcium pantothenate, pyridoxamine, and vitamin B12 (cobalamin). DSMZ Medium 461b Similar to DSMZ Meidum 461, except pyrrole-2-carboxylic acid and phosphate buffer are added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium461b.pdf 461b. MINERAL MEDIUM WITH PYRROLIC ACID Dissolve 10 mol pyrrole-2-carboxylic acid per liter potassium phosphate buffer (50 mM; pH 7.2) and sterilize the solution by membrane filtration. Prepare and autoclave medium 461 (vitamins not necessary). Add 10 ml of pyrrolic acid solution per liter mineral medium. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank mineral medium with pyrrolic acid DSMZ Medium 461 Carrine Blank DSM strains: mineral medium (Nagel and Andreesen) A minerals-salts, liquid culture medium comprised of sodium phosphate, potassium phosphate, calcium chloride, magnesium sulfate, manganese sulfate, ammonium chloride, sodium chloride, vitamins, and trace elements. Nagel and Andreesen mineral medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium461.pdf 461. MINERAL MEDIUM (NAGEL AND ANDREESEN) Distilled water 1000.00 ml Na2HPO4 x 2 H2O 1.45 g KH2PO4 0.25 g CaCl2 0.01 g MgSO4 x 7 H2O 0.50 g MnSO4 0.01 g NH4Cl 0.30 g NaCl 0.05 g Vitamin Solution 5.00 ml Trace element sol. SL-10 1.00 ml Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5. Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source. Vitamin solution: Vitamin B12 50.00 mg Pantothenic acid 50.00 mg Riboflavin 50.00 mg Pyridoxamine-HCl 10.00 mg Biotin 20.00 mg Folic acid 20.00 mg Nicotinic acid 25.00 mg Nicotine amide 25.00 mg α-lipoic acid 50.00 mg p-aminobenzoic acid 50.00 mg Thiamine-HCl x 2 H2O 50.00 mg Distilled water 1000.00 ml Stir for some hours, filter sterilize the solution. Trace element solution SL-10: HCl (25%; 7.7 M) 10.00 ml FeCl2 x 4 H2O 1.50 g ZnCl2 70.00 mg MnCl2 x 4 H2O 100.00 mg H3BO3 6.00 mg CoCl2 x 6 H2O 190.00 mg CuCl2 x 2 H2O 2.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 36.00 mg Distilled water 990.00 ml First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml. © 2010 DSMZ GmbH - All rights reserved vitamin solution for DSMZ Medium 461 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium461.pdf 461. MINERAL MEDIUM (NAGEL AND ANDREESEN) Distilled water 1000.00 ml Na2HPO4 x 2 H2O 1.45 g KH2PO4 0.25 g CaCl2 0.01 g MgSO4 x 7 H2O 0.50 g MnSO4 0.01 g NH4Cl 0.30 g NaCl 0.05 g Vitamin Solution 5.00 ml Trace element sol. SL-10 1.00 ml Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5. Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source. Vitamin solution: Vitamin B12 50.00 mg Pantothenic acid 50.00 mg Riboflavin 50.00 mg Pyridoxamine-HCl 10.00 mg Biotin 20.00 mg Folic acid 20.00 mg Nicotinic acid 25.00 mg Nicotine amide 25.00 mg α-lipoic acid 50.00 mg p-aminobenzoic acid 50.00 mg Thiamine-HCl x 2 H2O 50.00 mg Distilled water 1000.00 ml Stir for some hours, filter sterilize the solution. Trace element solution SL-10: HCl (25%; 7.7 M) 10.00 ml FeCl2 x 4 H2O 1.50 g ZnCl2 70.00 mg MnCl2 x 4 H2O 100.00 mg H3BO3 6.00 mg CoCl2 x 6 H2O 190.00 mg CuCl2 x 2 H2O 2.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 36.00 mg Distilled water 990.00 ml First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml. © 2010 DSMZ GmbH - All rights reserved A vitamin solution comprised of vitamin B12 (cobalamin), pantothenic acid, riboflavin, pyridoxamine hydrochloride, biotin, folic acid, nicotinic acid, nicotine amide (nicotinamide), alpha-lipoic acid (lipoic acid), p-aminobenzoic acid (4-aminobenzoic acid), and thiamine hydrochloride. Carrine Blank DSMZ Medium 461a DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium461a.pdf 461a. MINERAL MEDIUM WITH QUINOLINE Prepare an emulsion of 3 g/l quinoline in 50 mM phosphate buffer by stirring or ultrasonication. Autoclave in a gas tight vessel. Add 100 ml of this emulsion to 1 l autoclaved medium 461. © 2007 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 461, except quinoline is added. Carrine Blank mineral medium with quinoline DSMZ Medium 460 An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium sulfate, sodium chloride, yeast extract, ferric chloride, and sodium malate. Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium460.pdf 460. NITROGEN-FREE MEDIUM FOR PSEUDOMONAS STUTZERI K2HPO4 0.5 g MgSO4 x 7 H2O 0.2 g NaCl 0.1 g Yeast extract 0.2 g FeCl3 x 6 H2O 15.0 mg DL-Na-malate 6.6 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved nitrogen-free medium for Pseudomonas stutzeri DSMZ Medium 458 Carrine Blank phenol degrading medium for Bacillus stearothermophilus http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium458.pdf 458. MEDIUM FOR BACILLUS STEAROTHERMOPHILUS - PHENOL DEGRADING K2HPO4 0.50 g NH4Cl 1.00 g MgSO4 x 7 H2O 0.02 g Yeast extract 0.20 g Casamino acids 0.10 g Distilled water 900.00 ml pH 7.4 Autoclave for 15 min. Add 1 ml of sterile SL-4 (see medium 14) and 100 ml of filter-sterilized phenol-solution (4.7g/l). © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, yeast extract, casamino acids, trace elements, and phenol. medium for Bacillus stearothermophilus - phenol degrading DSM strains: DSMZ Medium 459 DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium459.pdf 459. SUCROSE-PEPTONE-MEDIUM Peptone 20.0 g Sucrose 20.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved An organic-rich, liquid culture medium comprised of peptone and sucrose. sucrose-peptone-medium DSMZ Medium 457d Carrine Blank Similar to DSMZ Medium 457, except biphenyl is added. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457d.pdf 457d. MEDIUM WITH BIPHENYL Prepare medium 457. Make a stock solution of 10 g/l biphenyl in ethanol and filter sterilize it using a cellulose filter membrane. Add an aliquot of this stock solution to a sterile flask so that the final concentration will be 0.25 g/l biphenyl, and let the ethanol evaporate. Add sterile medium to the crystal-layered flask. Alternatively, add biphenyl crystals directly to the liquid medium or to the lid of inversely incubated petri dishes containing 457-agar medium. © 2007 DSMZ GmbH - All rights reserved medium with biphenyl DSMZ Medium 457c Carrine Blank medium with chloroacrylic acid Similar to DSMZ Medium 457, except 3-chloroacrylic acid is added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457c.pdf 457c. MEDIUM WITH CHLOROACRYLIC ACID Prepare medium 457. Make a stock solution of 4 g/l 3-chloroacrylic acid in water, neutralize and filter sterilize. Add 20 ml of this stock solution to 1 l of autoclaved medium. For better growth, 1 g/l glucose may be supplied additionally or instead of the chloroacrylic acid. © 2007 DSMZ GmbH - All rights reserved DSM strains: DSMZ Medium 457b.2 DSMZ Medium 457b.2 -< for DSM 13022 DSM 13022 is Pseudomonas frederiksbergensis medium with fluoranthrene or phenanthrene Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457b.pdf 457b. MEDIUM WITH FLUORANTHENE OR PHENANTHRENE For DSM 7526, prepare medium 457 with 200 mg/l Tween 80. Prepare a solution of 2 g/l fluoranthene in acetone and filter sterilize using a cellulose filter membrane. Add 1 ml of this stock solution to the sterilized culture flask. Let acetone evaporate. Add 20 ml medium to the flask. Alternatively, the direct addition of about 1-2 mg fluoranthene to the medium is possible. For DSM 13022, prepare medium 457 with 0.3 g/l casamino acids. Add phenanthrene instead of fluoranthene to the culture vessel. 0.1 ml phenanthrene stock solution in acetone (5 mg/ml) are used per flask. © 2007 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 457, except casamino acids and phenanthrene are added. DSMZ Medium 457b.1 Similar to DSMZ Medium 457, except Tween 80 (polysorbate 80) and fluoranthene are added. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457b.pdf 457b. MEDIUM WITH FLUORANTHENE OR PHENANTHRENE For DSM 7526, prepare medium 457 with 200 mg/l Tween 80. Prepare a solution of 2 g/l fluoranthene in acetone and filter sterilize using a cellulose filter membrane. Add 1 ml of this stock solution to the sterilized culture flask. Let acetone evaporate. Add 20 ml medium to the flask. Alternatively, the direct addition of about 1-2 mg fluoranthene to the medium is possible. For DSM 13022, prepare medium 457 with 0.3 g/l casamino acids. Add phenanthrene instead of fluoranthene to the culture vessel. 0.1 ml phenanthrene stock solution in acetone (5 mg/ml) are used per flask. © 2007 DSMZ GmbH - All rights reserved DSM 7526 is Sphingobium quisquiliarum DSMZ Medium 457b.1 -< for DSM 7526 medium with fluoranthrene or phenanthrene Carrine Blank DSMZ Medium 457a Carrine Blank DSM strains: mineral medium with 2-chlorobenzoate http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457a.pdf 457a. MINERAL MEDIUM WITH 2-CHLOROBENZOATE Dissolve 78.3 g/l 2-chlorobenzoic acid in water by slow addition of concentrated NaOH (pH should reach 7.4). Filter-sterilize the solution and add 10.0 ml per liter autoclaved medium 457. Adjust the pH of the final medium to 7.4. © 2007 DSMZ GmbH - All rights reserved Similar to DSMZ Medium 457, except 2-chlorobenzoic acid (neutralized with sodium hydroxide) is added. DSMZ Medium 456 DSM strains: Carrine Blank KPL medium An organic-rich, solid culture medium comprised of lactic acid whey, filtered white table wine, glucose, galactose, Tween 80 (polysorbate 80), and agar. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium456.pdf 456. KPL MEDIUM Lactic acid whey (see below) 1000.0 ml White table wine (see below) 140.0 ml Glucose 10.0 g Galactose 10.0 g Tween 80 1.0 ml Agar 15.0 g Adjust pH to 5.5 Lactic acid whey: Adjust 10% skim milk to pH 5.5 with lactic acid and heat at 100˚C for 30 minutes. Remove precipitate by filtration. For preparing broth medium, adjust filtrate to pH 7.0 with 2 N NaOH and heat at 100˚C for 30 minutes. Remove precipitate by filtration. Adjust filtrate to pH 5.5 with 2 N HCl. White table wine: Filter-sterilize and add to autoclaved basal medium. © 2007 DSMZ GmbH - All rights reserved lactic acid whey http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium456.pdf 456. KPL MEDIUM Lactic acid whey (see below) 1000.0 ml White table wine (see below) 140.0 ml Glucose 10.0 g Galactose 10.0 g Tween 80 1.0 ml Agar 15.0 g Adjust pH to 5.5 Lactic acid whey: Adjust 10% skim milk to pH 5.5 with lactic acid and heat at 100˚C for 30 minutes. Remove precipitate by filtration. For preparing broth medium, adjust filtrate to pH 7.0 with 2 N NaOH and heat at 100˚C for 30 minutes. Remove precipitate by filtration. Adjust filtrate to pH 5.5 with 2 N HCl. White table wine: Filter-sterilize and add to autoclaved basal medium. © 2007 DSMZ GmbH - All rights reserved An undefined organic chemical mixture formed by heating diluted skim milk and lactic acid, then filtered. The pH is increased with sodium hydroxide, sample heated and re-filtered. The final pH is then adjusted by adding HCl (hydrogen chloride). Carrine Blank filtered white table wine Carrine Blank An undefined organic chemical mixture derived from the juice of the wine grape (the fruit of Vitis vinifera), formed through the process of maceration (homogenization) and fermentation, followed by filtration. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium456.pdf 456. KPL MEDIUM Lactic acid whey (see below) 1000.0 ml White table wine (see below) 140.0 ml Glucose 10.0 g Galactose 10.0 g Tween 80 1.0 ml Agar 15.0 g Adjust pH to 5.5 Lactic acid whey: Adjust 10% skim milk to pH 5.5 with lactic acid and heat at 100˚C for 30 minutes. Remove precipitate by filtration. For preparing broth medium, adjust filtrate to pH 7.0 with 2 N NaOH and heat at 100˚C for 30 minutes. Remove precipitate by filtration. Adjust filtrate to pH 5.5 with 2 N HCl. White table wine: Filter-sterilize and add to autoclaved basal medium. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 457 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457.pdf 457. MINERAL MEDIUM (BRUNNER) Na2HPO4 2.44 g KH2PO4 1.52 g (NH4)2SO4 0.50 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.05 g Trace element solution SL-4 (see below) 10.00 ml Distilled water 1000.00 ml Adjust pH to 6.9. Prepare a separate solution of the phosphates and autoclave separately. Combine the two solutions after cooling. Rehydrate and cultivate lyophilized cells in complex medium (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 457 with the appropriate carbon source. Trace element solution SL-4: EDTA 0.50 g FeSO4 x 7 H2O 0.20 g Trace element solution SL-6 (see below) 100.00 ml Distilled water 900.00 ml Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml © 2012 DSMZ GmbH - All rights reserved Brunner mineral medium A minerals-salts, liquid culture medium comprised of sodium phosphate, potassium phosphate, ammonium sulfate, magnesium sulfate, calcium chloride, and trace elements. mineral medium DSM strains: DSMZ Medium 454 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium454.pdf 454. EBA MEDIUM KH2PO4 0.20 g NaCl 1.00 g KCl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.25 g Trace element sol. SL-10 (see medium 320) 1.00 ml Resazurin 1.00 mg Na2SeO3 x 5 H2O 1.00 ml (0.26 g/ 1000 ml 10 mM NaOH) Glycine 2.00 g Distilled water 950.00 ml pH 7.2 to 7.4 Before inoculation add per 100 ml of medium: Biotin (10 mg/l) 0.20 ml (autoclave for 10 minutes under N2) NaHCO3 (84 g/l) 3.00 ml (autoclaved under CO2) Na2S (60 g/l) 1.00 ml (autoclaved under N2) © 2007 DSMZ GmbH - All rights reserved EBA medium Carrine Blank A minerals-salts, liquid culture medium comprised of potassium phosphate, sodium chloride, potassium chloride, magnesium sulfate, calcium chloride, ammonium chloride, trace elements, resazurin, sodium selenite, glycine, biotin, sodium bicarbonate, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. DSM strains: DSMZ Medium 455 AMB medium An organic-rich, liquid culture medium comprised of trypticase peptone, yeast extract, soluble starch, meat infusion (meat extract), glucose, cysteine, calcium chloride, magnesium sulfate, ammonium sulfate, and potassium phosphate. DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium455.pdf 455. AMB MEDIUM Trypticase peptone 4.00 g Yeast extract 5.00 g Starch, soluble 1.00 g Meat infusion 25.00 g Glucose 5.00 g Cysteine 1.00 g CaCl2 0.01 g MgSO4 0.20 g (NH4)2SO4 1.00 g K2HPO4 15.00 g Distilled water 1000.00 ml pH: 6.9. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSMZ Medium 453 An organic-rich, liquid culture medium comprised of peptone from meat (meat peptone), peptone from casein (casitone), yeast extract, sodium chloride, and D-glucose. Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium453.pdf 453. STANDARD I MEDIUM Peptone from meat 7.8 g Peptone from caseine 7.8 g Yeast extract 2.8 g NaCl 5.6 g D(+)-Glucose 1.0 g Distilled water 1000.0 ml Autoclave for 15 min at 121˚C. pH of the final medium is 7.5. © 2012 DSMZ GmbH - All rights reserved DSM strains: Standard I medium DSMZ Medium 452 Nakayama medium Glucose medium http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium452.pdf 452. GLUCOSE MEDIUM (NAKAYAMA) Glucose 10.00 g Peptone 10.00 g Yeast extract 15.00 g Agar 10.00 g Distilled water 980.00 ml Solution A 10.00 ml Solution B 10.00 ml Solution C 1.00 ml Solution A: KH2PO4 0.50 g K2HPO4 0.50 g Distilled water 100.00 ml Solution B: MgSO4 x 7 H2O 3.00 g NaCl 0.10 g MnSO4 x 5 H2O 0.10 g CuSO4 x 5 H2O 0.01 g Distilled water 100.00 ml Solution C: FeSO4 x 7 H2O 0.10 g Na3-Citrate 2.00 g Distilled water 100.00 ml Autoclave all solutions separately, final pH is 6.8. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank An organic-rich, solid culture medium comprised of glucose, peptone, yeast extract, agar, potassium phosphate, magnesium sulfate, sodium chloride, manganese sulfate, coppersulfate, ferrous sulfate, and sodium citrate. DSMZ DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf xxxx DSMZ Medium 449 M17 medium DSM strains: Carrine Blank M17 medium for lactic streptococci An organic-rich, liquid culture medium comprised of peptone from casein (casitone), soya peptone (soy peptone), peptone bacteriological (peptone), yeast extract, ascorbic acid, magnesium sulfate, sodium glycerophosphate, and lactose. http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium449.pdf 449. M17 MEDIUM FOR LACTIC STREPTOCOCCI Peptone from casein 5.00 g Soya peptone 5.00 g Peptone bacteriological 5.00 g Yeast extract 2.50 g Ascorbic acid 0.50 g MgSO4 x 7 H2O 0.25 g Na2-ß-glycerophosphate 19.00 g Lactose 5.00 g Distilled water 1000.00 ml Adjust pH to 6.9 ± 0.2. Lactose should be sterilized by filtration. © 2007 DSMZ GmbH - All rights reserved DSMZ Medium 450 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium450.pdf 450. SUPPLEMENTED (ARGININE) M9 MEDIUM To medium 382 add 20 mg/l arginine. © 2007 DSMZ GmbH - All rights reserved Supplemented (arginine) M9 medium DSM strains: M9 medium supplemented with arginine supplemented M9 medium Similar to DSMZ Medium 382, except arginine is added. DSMZ Medium 441 RBA http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium441.pdf 441. DIAZOTROPHIC MEDIUM (RBA) Solution A: KH2PO4 0.100 g K2HPO4 0.900 g NaCl 0.100 g CaCl2 x 2 H2O 0.100 g MgSO4 x 7 H2O 0.100 g Na2MoO4 x 2 H2O 0.005 g NaVO3 x H2O 0.005 g MnSO4 x H2O 0.005 g FeSO4 x 7 H2O 0.010 g Yeast extract 0.050 g Trace element sol. SL-6 (see medium 27) 3.000 ml Distilled water 950.000 ml Agar (if necessary) 15.000 g Adjust pH to 7.3. Solution B: Na2-succinate 1.000 g DL-Malate 2.000 g Na-pyruvate 1.000 g D-Mannitol 2.000 g D-Glucose 2.000 g Distilled water 50.000 ml Adjust pH to 7.3. Sterilize solution A separately at 121˚C for 15 min., cool to 50˚C and then mix aseptically with filter-sterilized solution B and 5.0 ml of filter-sterilized standard vitamin solution (see medium 428). RBA is an ammonium-free medium which has successfully been used for the isolation, growth and purity check of a broad spectrum of nitrogen fixing bacteria (Ref. 3363). For microaerophilic nitrogen-fixing bacteria use semisolid medium with 0.3% end concentration of agar and incubate the liquid cultures under 10% (v/v) air and 90% (v/v) N2. © 2007 DSMZ GmbH - All rights reserved DSM strains: Carrine Blank An organic-rich, liquid medium comprised of potassium phosphate, sodium chloride, calcium chloride, magnesium sulfate, sodium molybdate, sodium metavanadate, manganese sulfate, ferrous sulfate, yeast extract, and trace elements. Prepared under an atmosphere of air and dinitrogen. diazotrophic medium DSMZ Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSM strains: xxxx DSMZ xxxx http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf Carrine Blank DSM strains: DSMZ Carrine Blank xxxx http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSM strains: DSMZ xxxx Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSMZ http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSM strains: xxxx Carrine Blank DSMZ http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf Carrine Blank xxxx DSM strains: DSMZ http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf xxxx Carrine Blank DSM strains: DSMZ http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf Carrine Blank DSM strains: xxxx DSMZ xxxx http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSM strains: Carrine Blank DSMZ DSM strains: Carrine Blank xxxx http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSMZ DSM strains: xxxx Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSMZ DSM strains: Carrine Blank xxxx http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSMZ xxxx Carrine Blank DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSMZ Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSM strains: xxxx DSMZ http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSM strains: xxxx Carrine Blank DSMZ DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf xxxx DSMZ Carrine Blank xxxx http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSM strains: DSMZ xxxx http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf Carrine Blank DSM strains: DSMZ xxxx http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf Carrine Blank DSM strains: DSMZ DSM strains: xxxx Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSMZ xxxx DSM strains: Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSMZ DSM strains: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf xxxx Carrine Blank DSMZ Carrine Blank xxxx http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSM strains: DSMZ Carrine Blank xxxx http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSM strains: DSMZ xxxx Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf DSM strains: DSMZ Medium 784.1 Carrine Blank http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium784.pdf 784. NATRONIELLA MEDIUM KH2PO4 0.2 g MgCl2 x 6 H2O 0.1 g NH4Cl 1.0 g KCl 0.2 g NaCl 16.0 g Trace element solution SL-11 (see below) 1.0 ml Yeast extract 0.2 g Resazurin 0.5 mg Na2CO3 68.0 g NaHCO3 38.0 g Ethanol 5.0 ml Vitamin solution (see medium 141) 10.0 ml Na2S x 9 H2O 1.0 g Distilled water 1000.0 ml Trace element solution SL-11: Na2-EDTA 5.2 g FeCl2 x 4 H2O 1.5 g ZnCl2 70.0 mg MnCl2 x 4 H2O 100.0 mg H3BO3 6.0 mg CoCl2 x 6 H2O 190.0 mg CuCl2 x 2 H2O 2.0 mg NiCl2 x 6 H2O 24.0 mg Na2Mo4 x 2 H2O 36.0 mg Distilled water 1000.0 ml Adjust pH of solution to 6.0. Dissolve ingredients except carbonates, ethanol, vitamins and sulfide. Bring medium to the boil, then cool to room temperature while flushing with 100% N2 gas. Add and dissolve carbonates and sulfide while gassing the head space only. Dispense and autoclave under N2 gas atmosphere. Before use add ethanol and vitamins from sterile anoxic stock solution prepared under N2. Vitamins are sterilized by filtration. Adjust pH of the completed medium to 9.5 – 10.0. For DSM 24923 omit ethanol and add to the autoclaved medium 2.0 g/l Na2S2O3 x 5 H2O from a sterile anoxic stock solution. © 2013 DSMZ GmbH - All rights reserved DSM 24923 is Natranaerobaculum magadiense Similar to DSMZ Medium 784, except ethanol is omitted and sodium thiosulfate is added. DSMZ Medium 784.1 -< for DSM 24923 differentiated cyanobacterial filament A cyanobacterial filament that is differentiated. Carrine Blank prokaryotic colony Carrine Blank A colony comprised of a prokaryotic cells. cell pigmentation Prokaryotic cell quality, where the cell is pigmented (in that it has a color imparted by a chemical pigment compound). Carrine Blank cell granulation A prokaryotic cell quality, where the cell has intracellular granules. Carrine Blank cell size quality Prokaryotic cell quality, where the cell has a distinctive size (width). Carrine Blank thylakoid quality Carrine Blank A prokaryotic cell part quality, where the cell contains thylakoids membranes. pico-sized Cell size quality, where the width of the cell is between 0.2 and 2 microns (um). Carrine Blank nano-sized Carrine Blank Cell size quality, where the width of the cell is between 2 and 20 microns (um). filterability Carrine Blank Cell size quality, where the size of a prokaryotic cell is small enough to pass through a filter with a defined pore size. Berkefeld N filterable cell size A Berkefeld filterable cell size that is small enough to pass through a Berkefeld filter candle with an intermediate pore size N (for Normal). Pore sizes average 0.43 microns in diameter. Carrine Blank Berkefeld V filterable cell size Carrine Blank A Berkefeld filterable cell size that is small enough to pass through a Berkefeld filter candle with a smaller pore size V (for Viel). Pore sizes average 0.38 microns in diameter. This is the most common filter size used to study the filterability of microorganisms. Berkefeld filterable cell size Carrine Blank Cell size quality, where the cell has a filterable cell size that is small enough to pass through a Berkefeld filter candle with a defined pore size (e.g. V, N, or W), the largest of which is 0.45 microns. Berkefeld W filterable cell size A Berkefeld filterable cell size that is small enough to pass through a Berkefeld filter candle with a wider pore size W (for Wenig). Pore sizes average 0.45 microns in diameter. Carrine Blank RapID 32 Strep system A rapid commercial standardized kit containing 32 assays to distinguish streptococci and enterococci. Carrine Blank www.mediray.co.nz/.../om_biomerieux_test-kits_ot-32600_package_insert-32600.pdf "rapid ID 32 STREP is a standardized system for the identification of streptococci and enterococci, and those most common related organisms, in 4 hours, which uses 32 miniaturized enzymatic tests, as well as a specific database. The complete list of those organisms that it is possible to identify with this system is given in the Identification Table at the end of this package insert. Reading and interpretation are carried out automatically or manually." 2,4,6-trichlorophenol fermentation assay 2,4,6-trichlorophenol 2,4,6-trichlorophenol acidification 2,4,6-trichlorophenol fermentation Assays for the ability of a microorganism to ferment 2,4,6-trichlorophenol. Carrine Blank trichlorophenol 2,4-dichlorophenol fermentation assay Carrine Blank 2,4-dichlorophenol acidification dichlorophenol 2,4-dichlorophenol fermentation 2,4-dichlorophenol Assays for the ability of a microorganism to ferment 2,4-dichlorophenol. 2,5-didehydro-D-gluconic acid fermentation assay Assays for the ability of a microorganism to ferment 2,5-didehydro-D-gluconate (2,5-didehydro-D-gluconic acid). 2,5-didehydro-D-gluconic acid 2,5-didehydro-D-gluconate 2,5-didehydro-D-gluconate fermentation 2,5-diketogluconate Carrine Blank 2,5-didehydro-D-gluconate acidification 2,5-diketo-d-gluconate 2,5-dihydroxybenzoic acid fermentation assay Assays for the ability of a microorganism to ferment 2,5-dihydroxybenzoate (2,5-dihydroxybenzoic acid). 2,5-dihydroxybenzoate fermentation Carrine Blank gentisate 2,5-dihydroxybenzoic acid 2,5-dihydroxybenzoate acidification 2,5-dihydroxybenzoate 2,6-dihydroxybenzoic acid fermentation assay b-resorcylate Assays for the ability of a microorganism to ferment 2,6-dihydroxybenzoate (2,6-dihydroxybenzoic acid). 2,6-dihydroxybenzoate 2,6-dihydroxybenzoate acidification 2,6-dihydroxybenzoic acid beta-resorcylate 2,6-dihydroxybenzoate fermentation Carrine Blank 2-amino-2-deoxy-D-gluconic acid fermentation assay Carrine Blank 2-amino-2-deoxy-D-gluconate 2-amino-2-deoxy-D-gluconic acid glucosaminate 2-amino-2-deoxy-D-gluconate fermentation Assays for the ability of a microorganism to ferment 2-amino-2-deoxy-D-gluconate (2-amino-2-deoxy-D-gluconic acid). D-glucosaminic acid 2-amino-2-deoxy-D-gluconate acidification Carrine Blank 2,4,6-trichlorophenol assimilation assay 2,4,6-trichlorophenol Assays for the ability of a microorganism to assimilate 2,4,6-trichlorophenol as a sole source of carbon and energy. 2,4,6-trichlorophenol assimilation trichlorophenol Carrine Blank caffeic acid assimilation assay Assays for the ability of a microorganism to assimilate caffeic acid as a sole source of carbon and energy. caffeic acid assimilation Carrine Blank caffeic acid 2,6-dihydroxybenzoic acid assimilation assay Carrine Blank 2,6-dihydroxybenzoic acid 2,6-dihydroxybenzoate 2,6-dihydroxybenzoate assimilation b-resorcylate beta-resorcylate Assays for the ability of a microorganism to assimilate 2,6-dihydroxybenzoate (2,6-dihydroxybenzoic acid) as a sole source of carbon and energy. 2,5-dihydroxybenzoic acid assimilation assay 2,5-dihydroxybenzoate assimilation 2,5-dihydroxybenzoic acid Assays for the ability of a microorganism to assimilate 2,5-dihydroxybenzoate (2,5-dihydroxybenzoic acid) as a sole source of carbon and energy. 2,5-dihydroxybenzoate gentisate Carrine Blank 2,5-didehydro-D-gluconic acid assimilation assay 2,5-diketo-d-gluconate Assays for the ability of a microorganism to assimilate 2,5-didehydro-D-gluconate (2,5-didehydro-D-gluconic acid) as a sole source of carbon and energy. Carrine Blank 2,5-didehydro-D-gluconate assimilation 2,5-didehydro-D-gluconate 2,5-diketogluconate 2,4-dichlorophenol assimilation assay dichlorophenol 2,4-dichlorophenol assimilation 2,4-dichlorophenol Assays for the ability of a microorganism to assimilate 2,4-dichlorophenol as a sole source of carbon and energy. Carrine Blank RapID 32 A system A rapid commercial standardized kit containing 32 assays to distinguish anaerobic bacteria. Carrine Blank Clinical Microbiology and Infection Volume 5, Issue 6, June 1999, Pages 319-326 Evaluation of the Rapid ID 32A system for identification of anaerobic Gram-negative bacilli, excluding the Bacteroides fragilis group. J Downes, A King, J Hardie, and I Phillips. https://doi.org/10.1111/j.1469-0691.1999.tb00150.x cadaverine assimilation assay cadaverine cadaverine assimilation Assays for the ability of a microorganism to assimilate cadaverine as a sole source of carbon and energy. Carrine Blank butyric acid assimilation assay n-butyric butyrate assimilation Assays for the ability of a microorganism to assimilate butyrate as a sole source of carbon and energy. butyrate n-butyrate Carrine Blank n-butyric acid butanoic acid butanone assimilation assay Carrine Blank butanone assimilation Assays for the ability of a microorganism to assimilate butanone as a sole source of carbon and energy. butanone 4-hydroxybenzaldehyde assimilation assay 4-hydroxybenzaldehyde Carrine Blank Assays for the ability of a microorganism to assimilate 4-hydroxybenzaldehyde as a sole source of carbon and energy. 4-hydroxybenzaldehyde assimilation 4-hydroxy-L-proline assimilation assay Carrine Blank 4-hydroxy-L-proline hydroxy-L-proline hydroxy proline L-hydroxyproline Assays for the ability of a microorganism to assimilate 4-hydroxy-L-proline as a sole source of carbon and energy. 4-hydroxy-L-proline assimilation 4-coumaric acid assimilation assay 4-coumarate assimilation p-coumaric acid p-coumarate Assays for the ability of a microorganism to assimilate 4-coumarate (4-coumaric acid) as a sole source of carbon and energy. 4-coumaric acid 4-coumarate Carrine Blank 4-chlorophenol assimilation assay chlorophenol Assays for the ability of a microorganism to assimilate 4-chlorophenol as a sole source of carbon and energy. 4-chlorophenol Carrine Blank 4-chlorophenol assimilation 4-aminobenzoic acid assimilation assay Carrine Blank 4-aminobenzoate 4-aminobenzoic acid p-aminobenzoic acid Assays for the ability of a microorganism to assimilate 4-aminobenzoate (4-aminobenzoic acid) as a sole source of carbon and energy. 4-aminobenzoate assimilation para-aminobenzoate 3-phenylpropionic acid assimilation assay Carrine Blank 3-phenylpropionate phenylpropionate 3-phenylpropionic acid 3-phenylpropionate assimilation hydrocinnamate Assays for the ability of a microorganism to assimilate 3-phenylpropionate (3-phenylpropionic acid) as a sole source of carbon and energy. phenylpropionic acid phenyl propionic acid 3-phenyllactic acid assimilation assay 3-phenyllactic acid Carrine Blank Assays for the ability of a microorganism to assimilate 3-phenyllactate (3-phenyllactic acid) as a sole source of carbon and energy. phenyllactate 3-phenyllactate phenyl lactic acid 3-phenyllactate assimilation azelaic acid assimilation assay Assays for the ability of a microorganism to assimilate azelaate (azelaic acid) as a sole source of carbon and energy. Carrine Blank azelaate azelaic acid azelaate assimilation 3-hydroxy-4-methoxybenzoic acid assimilation assay Assays for the ability of a microorganism to assimilate 3-hydroxy-4-methoxybenzoate (3-hydroxy-4-methoxybenzoic acid) as a sole source of carbon and energy. isovanillate 3-hydroxy-4-methoxybenzoate assimilation 3-hydroxy-4-methoxybenzoic acid Carrine Blank 3-hydroxy-4-methoxybenzoate 3-coumaric acid assimilation assay 3-coumarate 3-coumaric acid Carrine Blank m-coumarate 3-coumarate assimilation Assays for the ability of a microorganism to assimilate 3-coumarate (3-coumaric acid) as a sole source of carbon and energy. 3-aminobenzoic acid assimilation assay Assays for the ability of a microorganism to assimilate 3-aminobenzoate (3-aminobenzoic acid) as a sole source of carbon and energy. 3-aminobenzoic acid Carrine Blank 3-aminobenzoate 3-aminobenzoate assimilation 3,4-dimethoxybenzoic acid assimilation assay dimethoxybenzoic acid Carrine Blank 3,4-dimethoxybenzoic acid Assays for the ability of a microorganism to assimilate 3,4-dimethoxybenzoic acid as a sole source of carbon and energy. 3,4-dimethoxybenzoic acid assimilation dimethoxybenzoate 3,4-dimethoxybenzoate veratrate 3-(3,4-dihydroxyphenyl)propanoic acid assimilation assay 3-(3,4-dihydroxyphenyl)propanoic acid assimilation 3-(3,4-dihydroxyphenyl)propanoate Carrine Blank 3-(3,4-dihydroxyphenyl)propanoic acid Assays for the ability of a microorganism to assimilate 3-(3,4-dihydroxyphenyl)propanoic acid or 3-(3,4-dihydroxyphenyl)propanoate as a sole source of carbon and energy. 3,4-dihydroxybenzoic acid assimilation assay dihydroxybenzoic acid 3,4-dihydroxybenzoate 3,4-dihydroxybenzoate assimilation Carrine Blank 3,4-dihydroxybenzoic acid protocatechuate Assays for the ability of a microorganism to assimilate 3,4-dihydroxybenzoate (3,4-dihydroxybenzoic acid) as a sole source of carbon and energy. dihydroxybenzoate 3,4,5-trimethoxycinnamic acid assimilation assay 3,4,5-trimethoxycinnamate trimethoxycinnamic acid Assays for the ability of a microorganism to assimilate 3,4,5-trimethoxycinnamate (3,4,5-trimethoxycinnamic acid) as a sole source of carbon and energy. 3,4,5-trimethoxycinnamate assimilation 3,4,5-trimethoxycinnamic acid Carrine Blank tmc trimethoxycinnamate 3,4,5-trimethoxybenzoic acid assimilation assay trimethoxybenzoic acid Carrine Blank trimethoxybenzoate 3,4,5-trimethoxybenzoic acid 3,4,5-trimethoxybenzoate 3,4,5-trimethoxybenzoate assimilation Assays for the ability of a microorganism to assimilate 3,4,5-trimethoxybenzoate (3,4,5-trimethoxybenzoic acid) as a sole source of carbon and energy. 2-phenylethylamine assimilation assay b-phenylethylamine 2-phenylethylamine Assays for the ability of a microorganism to assimilate 2-phenylethylamine as a sole source of carbon and energy. Carrine Blank 2-phenylethylamine assimilation 2-phenylethanol assimilation assay 2-phenylethanol phenylethanol phenylethyl alcohol 2-phenylethanol assimilation Assays for the ability of a microorganism to assimilate 2-phenylethanol as a sole source of carbon and energy. Carrine Blank 2-methylbutyric acid assimilation assay methyl-2-butyrate methyl 2-butyrate Assays for the ability of a microorganism to assimilate 2-methylbutyrate (2-methylbutyric acid) as a sole source of carbon and energy. Carrine Blank 2-methylbutyrate assimilation 2-methylbutyrate 2-methylbutyric acid 2-methoxyethanol assimilation assay 2-methoxyethanol assimilation 2-methoxyethanol Carrine Blank Assays for the ability of a microorganism to assimilate 2-methoxyethanol as a sole source of carbon and energy. methoxyethanol 2-ethoxyethanol assimilation assay Assays for the ability of a microorganism to assimilate 2-ethoxyethanol as a sole source of carbon and energy. ethoxyethanol 2-ethoxyethanol Carrine Blank 2-ethoxyethanol assimilation 2-deoxy-D-glucose assimilation assay Carrine Blank 2-deoxyglucose 2-deoxy-D-glucose assimilation 2-deoxy-D-glucose Assays for the ability of a microorganism to assimilate 2-deoxy-D-glucose as a sole source of carbon and energy. 2-coumaric acid assimilation assay 2-coumaric acid 2-coumarate assimilation Assays for the ability of a microorganism to assimilate 2-coumarate (2-coumaric acid) as a sole source of carbon and energy. o-coumarate 2-coumarate Carrine Blank 2-aminopentanoic acid assimilation assay norvaline 2-aminopentanoic acid Assays for the ability of a microorganism to assimilate 2-aminopentanoic acid as a sole source of carbon and energy. Carrine Blank 2-aminopentanoic acid assimilation 2-aminopentanoate isovanillin assimilation assay isovanillin isovanillin assimilation Carrine Blank Assays for the ability of a microorganism to assimilate isovanillin as a sole source of carbon and energy. 3-hydroxy-4-methoxybenzaldehyde 2-aminopentanoic acid fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment 2-aminopentanoic acid. 2-aminopentanoic acid fermentation 2-aminopentanoic acid acidification 2-aminopentanoic acid norvaline 2-coumaric acid fermentation assay 2-coumarate o-coumaric acid Assays for the ability of a microorganism to ferment 2-coumarate (2-coumaric acid). 2-coumarate fermentation 2-coumaric acid ortho-coumarate Carrine Blank 2-coumarate acidification o-coumarate 2-deoxy-D-glucose fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment 2-deoxy-D-glucose. 2-deoxy-D-glucose fermentation 2-deoxy-D-glucose 2-deoxy-D-glucose acidification 2-deoxyglucose 2-ethoxyethanol fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment 2-ethoxyethanol. 2-ethoxyethanol 2-ethoxyethanol fermentation 2-ethoxyethanol acidification 2-hydroxybutyric acid fermentation assay 2-hydroxybutyric acid Assays for the ability of a microorganism to ferment 2-hydroxybutyrate (2-hydroxybutyric acid). Carrine Blank 2-hydroxybutyrate fermentation 2-hydroxybutyrate acidification 2-hydroxybutyrate a-hydroxy-butyric acid a-hydroxybutyrate ahydroxybutyric acid a-hydroxybutyric acid 2-methoxyethanol fermentation assay Assays for the ability of a microorganism to ferment 2-methoxyethanol. methoxyethanol 2-methoxyethanol fermentation Carrine Blank 2-methoxyethanol 2-methoxyethanol acidification 2-methylbutyric acid fermentation assay Carrine Blank methyl-2-butyrate 2-methylbutyric acid 2-methylbutyrate fermentation 2-methylbutyrate Assays for the ability of a microorganism to ferment 2-methylbutyrate (2-methylbutyric acid). methyl 2-butyrate 2-methylbutyrate acidification 2-oxobutanoic acid fermentation assay 2-oxobutanoic acid alpha-ketobutyric acid a-ketobutyrate Assays for the ability of a microorganism to ferment 2-oxobutanoate (2-oxobutanoic acid). a-keto-butyric acid Carrine Blank 2-oxobutanoate 2-oxobutanoate fermentation 2-oxobutanoate acidification 2-oxoglutaric acid fermentation assay sodium a-ketoglutarate a-keto-glutaric acid 2-oxoglutarate Carrine Blank 2-oxoglutaric acid a-ketoglutaric acid 2-oxoglutaric acid acidification Assays for the ability of a microorganism to ferment 2-oxoglutaric acid (2-oxoglutarate). 2-oxoglutaric acid fermentation a-ketoglutarate 2-oxopentanoic acid fermentation assay 2-oxopentanoate acidification a-ketovalerate 2-oxopentanoate 2-oxopentanoic acid 2-oxopentanoate fermentation Carrine Blank 2-ketovalerate a-ketovaleric acid Assays for the ability of a microorganism to ferment 2-oxopentanoate (2-oxopentanoic acid). 2-phenylethanol fermentation assay 2-phenylethanol acidification 2-phenylethanol Assays for the ability of a microorganism to ferment 2-phenylethanol. 2-phenylethanol fermentation phenylethanol Carrine Blank phenylethyl alcohol 2-phenylethylamine fermentation assay 2-phenylethylamine 2-phenylethylamine fermentation Assays for the ability of a microorganism to ferment 2-phenylethylamine. b-phenylethylamine 2-phenylethylamine acidification Carrine Blank 2'-deoxyadenosine fermentation assay 2-deoxyadenosine 29-deoxyadenosine dA deoxyadenosine Carrine Blank Assays for the ability of a microorganism to ferment 2'-deoxyadenosine. 2'-deoxyadenosine fermentation 2'-deoxyadenosine acidification 2'-deoxyadenosine 3,4,5-trimethoxybenzoic acid fermentation assay Assays for the ability of a microorganism to ferment 3,4,5-trimethoxybenzoate (3,4,5-trimethoxybenzoic acid). Carrine Blank 3,4,5-trimethoxybenzoate fermentation trimethoxybenzoic acid 3,4,5-trimethoxybenzoate acidification trimethoxybenzoate 3,4,5-trimethoxybenzoate 3,4,5-trimethoxybenzoic acid 3,4,5-trimethoxycinnamic acid fermentation assay 3,4,5-trimethoxycinnamic acid Carrine Blank 3,4,5-trimethoxycinnamate acidification trimethoxycinnamate Assays for the ability of a microorganism to ferment 3,4,5-trimethoxycinnamate (3,4,5-trimethoxycinnamic acid). trimethoxycinnamic acid 3,4,5-trimethoxycinnamate fermentation 3,4,5-trimethoxycinnamate tmc 3,4-dihydroxybenzoic acid fermentation assay 3,4-dihydroxybenzoic acid 3,4-dihydroxybenzoate 3,4-dihydroxybenzoate fermentation Carrine Blank protocatechuate Assays for the ability of a microorganism to ferment 3,4-dihydroxybenzoate (3,4-dihydroxybenzoic acid). 3,4-dihydroxybenzoate acidification 3-(3,4-dihydroxyphenyl)propanoic acid fermentation assay 3-(3,4-dihydroxyphenyl)propanoic acid 3-(3,4-dihydroxyphenyl)propanoic acid acidification 3-(3,4-dihydroxyphenyl)propanoic acid fermentation Carrine Blank 3-(3,4-dihydroxyphenyl)propanoate Assays for the ability of a microorganism to ferment 3-(3,4-dihydroxyphenyl)propanoic acid (or 3-(3,4-dihydroxyphenyl)prpanoate). 3,4-dimethoxybenzoic acid fermentation assay veratrate 3,4-dimethoxybenzoic acid 3,4-dimethoxybenzoic acid fermentation 3,4-dimethoxybenzoic acid acidification Assays for the ability of a microorganism to ferment 3,4-dimethoxybenzoic acid. Carrine Blank 3-aminobenzoic acid fermentation assay Carrine Blank 3-aminobenzoate acidification 3-aminobenzoate fermentation Assays for the ability of a microorganism to ferment 3-aminobenzoate (3-aminobenzoic acid). 3-aminobenzoic acid 3-aminobenzoate 3-coumaric acid fermentation assay 3-coumaric acid m-coumaric acid 3-coumarate acidification meso-coumarate 3-coumarate Carrine Blank m-coumarate 3-coumarate fermentation Assays for the ability of a microorganism to ferment 3-coumarate (3-coumaric acid). 3-hydroxy-4-methoxybenzoic acid fermentation assay Carrine Blank isovanillate 3-hydroxy-4-methoxybenzoate 3-hydroxy-4-methoxybenzoate acidification 3-hydroxy-4-methoxybenzoic acid Assays for the ability of a microorganism to ferment 3-hydroxy-4-methoxybenzoate (3-hydroxy-4-methoxybenzoic acid). 3-hydroxy-4-methoxybenzoate fermentation 3-hydroxybenzoic acid fermentation assay 3-hydroxybenzoate Assays for the ability of a microorganism to ferment 3-hydroxybenzoate (3-hydroxybenzoic acid). 3-hydroxybenzoate fermentation Carrine Blank m-hydroxybenzoate 3-hydroxybenzoate acidification 3-hydroxybenzoic acid 3-hydroxybutyric acid fermentation assay 3-hydroxybutyrate fermentation Carrine Blank DL-b-hydroxybutyrate 3-hydroxybutyric acid b-hydroxybutyrate 3-hydroxybutyrate acidification DL-3-hydroxybutyrate b-hydroxy-L-butyric acid hydroxy-b-DL-butyric acid Assays for the ability of a microorganism to ferment 3-hydroxybutyrate (3-hydroxybutyric acid). b-hydroxy butyric acid 3-hydroxybutyrate DL-carnitine 3-O-methyl-D-glucose fermentation assay 3-methyl D-glucopyranoside methyl glucose 3-O-methyl-D-glucose Carrine Blank 3-O-methyl-D-glucosex fermentation 3-O-methyl-D-glucose acidification Assays for the ability of a microorganism to ferment 3-O-methyl-D-glucose. 3-methyl D-glucose 3-methyl glucose 3-O-methyl-D-glucopyranose 3-phenyllactic acid fermentation assay 3-phenyllactic acid phenyl lactic acid Carrine Blank 3-phenyllactate acidification 3-phenyllactate 3-phenyllactate fermentation Assays for the ability of a microorganism to ferment 3-phenyllactate (3-phenyllactic acid). 3-phenylpropionic acid fermentation assay 3-phenylpropionate fermentation Assays for the ability of a microorganism to ferment 3-phenylpropionate (3-phenylpropionic acid). hydrocinnamate phenylpropionate 3-phenylpropionic acid phenyl propionic acid 3-phenylpropionate acidification phenylpropionic acid 3-phenylpropionate Carrine Blank 4-aminobenzoic acid fermentation assay 4-aminobenzoate acidification Assays for the ability of a microorganism to ferment 4-aminobenzoate (4-aminobenzoic acid). p-aminobenzoic acid Carrine Blank para-aminobenzoate 4-aminobenzoate 4-aminobenzoate fermentation 4-aminobenzoic acid 4-chlorophenol fermentation assay Assays for the ability of a microorganism to ferment 4-chlorophenol. 4-chlorophenol acidification chlorophenol 4-chlorophenol Carrine Blank 4-chlorophenol fermentation 4-coumaric acid fermentation assay Carrine Blank p-coumarate 4-coumarate acidification p-coumaric acid 4-coumarate 4-coumarate fermentation 4-coumaric acid para-coumarate Assays for the ability of a microorganism to ferment 4-coumarate (4-coumaric acid). 4-hydroxy-L-proline fermentation assay Assays for the ability of a microorganism to ferment 4-hydroxy-L-proline. 4-hydroxy-L-proline acidification hydroxy proline 4-hydroxy-L-proline hydroxy-L-proline L-hydroxyproline Carrine Blank 4-hydroxy-L-proline fermentation 4-hydroxybenzaldehyde fermentation assay 4-hydroxybenzaldehyde 4-hydroxybenzaldehyde fermentation Carrine Blank 4-hydroxybenzaldehyde acidification Assays for the ability of a microorganism to ferment 4-hydroxybenzaldehyde. 4-hydroxybenzoic acid fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment 4-hydroxybenzoate (4-hydroxybenzoic acid). p-hydroxybenzoate 4-hydroxybenzoate fermentation 4-hydroxybenzoate acidification 4-hydroxybenzoic acid 4-hydroxybenzoate L-arginine fermentation assay Largine L-arginine L-arginine fermentation L-arginine acidification Assays for the ability of a microorganism to ferment L-arginine. Carrine Blank 4-hydroxybutyric acid fermentation assay g-hydroxybutyric acid g-hydroxy butyric acid 4-hydroxybutyrate g-hydroxybutyrate Carrine Blank 4-hydroxybutyrate fermentation 4-hydroxybutyric acid Assays for the ability of a microorganism to ferment 4-hydroxybutyrate (4-hydroxybutyric acid). 4-hydroxybutyrate acidification 4-hydroxyphenylacetic acid fermentation assay 4-hydroxyphenylacetate acidification p-hydroxy-phenylacetic acid 4-hydroxyphenylacetic acid p-hydroxyphenylacetic acid 4-hydroxyphenylacetate 4-hydroxyphenylacetate fermentation p-hydroxy-phenylactic acid Assays for the ability of a microorganism to ferment 4-hydroxyphenylacetate (4-hydroxyphenylacetic acid). Carrine Blank 4-methoxycinnamic acid fermentation assay 4-methoxycinnamic acid Assays for the ability of a microorganism to ferment 4-methoxycinnamic acid. Carrine Blank 4-methoxycinnamic acid fermentation 4-methoxycinnamic acid acidification 4-oxopentanoic acid fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment 4-oxopentanoate (4-oxopentanoic acid). 4-oxopentanoate fermentation 4-oxopentanoic acid g-ketovalerate 4-oxopentanoate 4-ketovalerate 4-oxopentanoate acidification 5-aminopentanoic acid fermentation assay Carrine Blank 5-aminovalerate 5-aminopentanoate acidification 5-aminopentanoate Assays for the ability of a microorganism to ferment 5-aminopentanoate (5-aminopentanoic acid). 5-aminopentanoic acid 5-aminopentanoate fermentation 5-oxoproline fermentation assay L-pyroglutamic acid 5-oxoprolinate fermentation pyroglutamic acid pyroglutamate 5-oxoprolinate 5-oxoprolinate acidification pyrrolidonyl 5-oxoproline Assays for the ability of a microorganism to ferment 5-oxoprolinate (5-oxoproline). Carrine Blank 6-O-alpha-D-glucopyranosyl-D-fructofuranose fermentation assay 6-O-alpha-D-glucopyranosyl-D-fructofuranose fermentation 6-O-alpha-D-glucopyranosyl-D-fructofuranose acidification 6-O-alpha-D-glucopyranosyl-D-fructofuranose Carrine Blank isomaltulose palatinose Assays for the ability of a microorganism to ferment 6-O-alpha-D-glucopyranosyl-D-fructofuranose. 6-O-acetyl-D-glucose fermentation assay N-acetyl-glucose 6-O-acetyl-D-glucose 6-O-acetyl-D-glucose fermentation 6-O-acetyl-D-glucose acidification Carrine Blank N-acetyl-glucopyranoside N-acetyl-glucoside Assays for the ability of a microorganism to ferment 6-O-acetyl-D-glucose. alpha-cyclodextrin fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment alpha-cyclodextrin. CDEX alpha-cyclodextrin alphacyclodextrin alpha-cyclodextrin acidification alpha-cyclodextrin fermentation a-cyclodextrin acyclodextrin acetamide fermentation assay acetamide acidification acetamide fermentation Assays for the ability of a microorganism to ferment acetamide. Carrine Blank acetamide acetic acid fermentation assay potassium acetate acetate acidification acetic acetic acid acetate fermentation actate acetate Assays for the ability of a microorganism to ferment acetate (acetic acid). sodium acetate Carrine Blank acetoacetic acid fermentation assay acetoacetic acid b-ketoglutaric acid 3-oxoglutaric acid acetoacetate fermentation acetoacetate acidification acetoacetate beta-ketoglutaric acid diactic acid acetonedicarboxylic acid Carrine Blank Assays for the ability of a microorganism to ferment acetoacetate (acetoacetic acid). acetoin fermentation assay Assays for the ability of a microorganism to ferment acetoin. acetoin acidification acetoin Carrine Blank acetoin fermentation acetone fermentation assay acetone acidification Assays for the ability of a microorganism to ferment acetone. acetone fermentation Carrine Blank acetone aconitic acid fermentation assay Assays for the ability of a microorganism to ferment aconitic acid (aconitate). aconitic acid fermentation aconitic acid acidification aconitic acid Carrine Blank aconitate acrylic acid fermentation assay Carrine Blank acrylic acid acrylate acrylate fermentation Assays for the ability of a microorganism to ferment acrylate (acrylic acid). acrylate acidification adenine fermentation assay Assays for the ability of a microorganism to ferment adenine. adenine fermentation adenine adenine acidification Carrine Blank adenosine fermentation assay adenosine Carrine Blank adenosine fermentation Assays for the ability of a microorganism to ferment adenosine. adenosine acidification adenosine 5'-monophosphate fermentation assay AMP acidification adenosine monophosphate adenosine 5'-monophosphate acidification Assays for the ability of a microorganism to ferment adenosine 5'-monophosphate. adenosine 5'-monophosphate adenosine 5'-monophosphate fermentation AMP Carrine Blank adipic acid fermentation assay adipinate Carrine Blank Assays for the ability of a microorganism to ferment adipic acid (adipate). adipic acid acidification adipic acid adipate adipic acid fermentation agar fermentation assay agar acidification Assays for the ability of a microorganism to ferment agar. Carrine Blank agar agar fermentation agarose fermentation assay Carrine Blank agarose fermentation agarose acidification agarose Assays for the ability of a microorganism to ferment agarose. Ala-Gly fermentation assay Ala-Gly acidification L-alanyl-glycine Ala-Gly fermentation Assays for the ability of a microorganism to ferment Ala-Gly. Ala-Gly L-Ala-Gly alanyl glycine Carrine Blank L-alanyl-L-glutamic acid fermentation assay alanyl glutamate L-alanyl-L-glutamic acid Carrine Blank Ala-Glu alanyl glutamic acid L-alanyl-L-glutamic acid fermentation L-alanyl-L-glutamic acid acidification Assays for the ability of a microorganism to ferment L-alanyl-L-glutamic acid. Ala-His fermentation assay Ala-His fermentation Ala-His Assays for the ability of a microorganism to ferment Ala-His. Ala-His acidification alanyl histidine Carrine Blank Ala-Thr fermentation assay Ala-Thr acidification Carrine Blank Ala-Thr fermentation Assays for the ability of a microorganism to ferment Ala-Thr. alanyl threonine Ala-Thr alanine fermentation assay alanine fermentation D- and L-alanine alanine acidification D,L-alanine alanine Assays for the ability of a microorganism to ferment alanine. Carrine Blank alginic acid fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment alginate (alginic acid). alginate acidification alginate sodium alginate alginate fermentation alginic acid allantoin fermentation assay Assays for the ability of a microorganism to ferment allantoin. allantoin fermentation allantoin Carrine Blank allantoin acidification aminobenzoic acid fermentation assay Carrine Blank aminobenzoate acidification aminobenzoate aminobenzoate fermentation aminobenzoic acid Assays for the ability of a microorganism to ferment aminobenzoate (aminobenzoic acid). ammonium betaine fermentation assay betaine-H2O betain betaine ammonium betaine ammonium betaine acidification Assays for the ability of a microorganism to ferment ammonium betaine. Carrine Blank ammonium betaine fermentation amylopectin fermentation assay Carrine Blank amylopectin amylopectin fermentation amylopectin acidification Assays for the ability of a microorganism to ferment amylopectin. amylose fermentation assay Assays for the ability of a microorganism to ferment amylose. amylose Carrine Blank amylose acidification amylose fermentation anthranilic acid fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment anthranilate. 2-aminobenzoate anthranilate acidification anthranilate anthranilate fermentation arabinitol fermentation assay arabinitol acidification arabinitol Assays for the ability of a microorganism to ferment arabinitol. Carrine Blank arabinitol fermentation arabitol arabinogalactan fermentation assay Carrine Blank arabinogalactan larch arabinogalactan arabinogalactan acidification Assays for the ability of a microorganism to ferment arabinogalactan. arabinogalactan fermentation arabinose fermentation assay Carrine Blank D- and L-arabinose Assays for the ability of a microorganism to ferment arabinose. D- or L-arabinose arabinose acidification arabinose arabiinose L- or D-arabinose ARA DL-arabinose arabinose fermentation arginine fermentation assay arginine acidification arginine fermentation Assays for the ability of a microorganism to ferment arginine. arginien Carrine Blank DL-arginine arginine ascorbic acid fermentation assay ascorbate ascorbate fermentation L-ascorbate ascorbic acid acidification ascorbate acidification ascorbic acid Carrine Blank Assays for the ability of a microorganism to ferment ascorbate (ascorbic acid). ascorbic acid fermentation asparagine fermentation assay asparagine acidification asparagine asparagine fermentation Assays for the ability of a microorganism to ferment asparagine. Carrine Blank aspartic acid fermentation assay aspartic acid fermentation aspartate Carrine Blank aspartic acid acidification aspartic acid Assays for the ability of a microorganism to ferment aspartic acid (aspartate). asparatate azelaic acid fermentation assay azelaic acid azelaate acidification Assays for the ability of a microorganism to ferment azelaate (azelaic acid). azelaic acid fermentation azelaic acid acidification azelaate fermentation Carrine Blank azelaate methyl D-galactoside assimilation assay Carrine Blank methyl D-galactoside assimilation methyl D-galactoside Assays for the ability of a microorganism to assimilate methyl D-galactoside as a sole source of carbon and energy. beta-alanine fermentation assay beta-alanine fermentation beta-alaninate 3-minopropanoate 3-aminopropanoate Assays for the ability of a microorganism to ferment beta-alanine (beta-alaninate). beta-alanine acidification Carrine Blank beta-alanine beta-cyclodextrin fermentation assay beta-cyclodextrin Carrine Blank Assays for the ability of a microorganism to ferment beta-cyclodextrin. beta-cyclodextrin acidification beta-cyclodextrin fermentation beta-D-glucuronamide fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment beta-D-glucuronamide. glucuronamide beta-D-glucuronamide fermentation beta-D-glucuronamide beta-D-glucuronamide acidification b-D-glucuronamide benzoic acid fermentation assay benzoic acid Assays for the ability of a microorganism to ferment benzoate (benzoic acid). benzoate benzoate acidification sodium benzoate benzoate fermentation Carrine Blank bromosuccinic acid fermentation assay Carrine Blank bromosuccinate fermentation bromosuccinate acidification bromosuccinic acid Assays for the ability of a microorganism to ferment bromosuccinate (bromosuccinic acid). bromosuccinate butan-1-ol fermentation assay Assays for the ability of a microorganism to ferment butan-1-ol. 1-butanol butan-1-ol Carrine Blank butan-1-ol acidification butan-1-ol fermentation butanol n-butanol butane-2,3-dione assimilation assay butane-2,3-dione assimilation Assays for the ability of a microorganism to assimilate butane-2,3-dione as a sole source of carbon and energy. Carrine Blank butane-2,3-dione diacetyl butane-1,3-diol assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate butane-1,3-diol as a sole source of carbon and energy. butane-1,3-diol butane-1,3-diol assimilation butan-1-ol assimilation assay Carrine Blank n-butanol butanol butan-1-ol assimilation Assays for the ability of a microorganism to assimilate butan-1-ol as a sole source of carbon and energy. butan-1-ol methyl D-glucoside fermentation assay methyl-D-glucopyranoside methyl D-glucoside acidification D-methyl glucoside MdG methyl D-glucoside Carrine Blank Assays for the ability of a microorganism to ferment methyl D-glucoside. MDG methyl D-glucoside fermentation methyl glucopyranoside benzoic acid assimilation assay benzoate assimilation sodium benzoate benzoate Assays for the ability of a microorganism to assimilate benzoate (benzoic acid) as a sole source of carbon and energy. Carrine Blank benzoic acid beta-alanine assimilation assay beta-alanine Assays for the ability of a microorganism to assimilate beta-alanine (beta-alaninate) as a sole source of carbon and energy. Carrine Blank 3-minopropanoate 3-aminopropanoate beta-alanine assimilation beta-alaninate methyl D-glucoside assimilation assay Carrine Blank methyl D-glucoside assimilation D-methyl glucoside methyl-D-glucopyranoside methyl glucopyranoside Assays for the ability of a microorganism to assimilate methyl D-glucoside as a sole source of carbon and energy. methyl D-glucoside aspartic acid assimilation assay aspartic acid aspartic acid assimilation aspartate Assays for the ability of a microorganism to assimilate aspartic acid (aspartate) as a sole source of carbon and energy. asparatate Carrine Blank aspartate assimilation asparagine assimilation assay Carrine Blank asparagine asparagine assimilation Assays for the ability of a microorganism to assimilate asparagine as a sole source of carbon and energy. ascorbic acid assimilation assay ascorbate assimilation ascorbate L-ascorbate Assays for the ability of a microorganism to assimilate ascorbate as a sole source of carbon and energy. Carrine Blank arginine assimilation assay arginine Carrine Blank DL-arginine arginine assimilation Assays for the ability of a microorganism to assimilate arginine as a sole source of carbon and energy. arginien arabinose assimilation assay arabinose D- or L-arabinose Assays for the ability of a microorganism to assimilate arabinose as a sole source of carbon and energy. DL-arabinose arabinose assimilation Carrine Blank D- and L-arabinose arabiinose L- or D-arabinose ARA arabinogalactan assimilation assay larch arabinogalactan Carrine Blank Assays for the ability of a microorganism to assimilate arabinogalactan as a sole source of carbon and energy. arabinogalactan assimilation arabinogalactan arabinitol assimilation assay D- or L-arabitol arabinitol assimilation D- and L-arabitol Carrine Blank arabinitol Assays for the ability of a microorganism to assimilate arabinitol as a sole source of carbon and energy. arabitol assimilation DL-arabitol arabitol anthranilic acid assimilation assay anthranilate Carrine Blank Assays for the ability of a microorganism to assimilate anthranilate as a sole source of carbon and energy. anthranilate assimilation 2-aminobenzoate amylose assimilation assay Assays for the ability of a microorganism to assimilate amylose as a sole source of carbon and energy. amylose amylose assimilation Carrine Blank amylopectin assimilation assay amylopectin assimilation Assays for the ability of a microorganism to assimilate amylopectin as a sole source of carbon and energy. amylopectin Carrine Blank ammonium betaine assimilation assay ammonium betaine betaine betain Assays for the ability of a microorganism to assimilate ammonium betaine as a sole source of carbon and energy. ammonium betaine assimilation betaine-H2O Carrine Blank aminobenzoic acid assimilation assay aminobenzoate assimilation aminobenzoate aminobenzoic acid Carrine Blank Assays for the ability of a microorganism to assimilate aminobenzoate (aminobenzoic acid) as a sole source of carbon and energy. allantoin assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate allantoin as a sole source of carbon and energy. allantoin allantoin assimilation alginic acid assimilation assay alginic acid Assays for the ability of a microorganism to assimilate alginate (alginic acid) as a sole source of carbon and energy. Carrine Blank alginate sodium alginate alginate assimilation alanine assimilation assay D,L-alanine ALA alanine D- and L-alanine Carrine Blank alanine assimilation Assays for the ability of a microorganism to assimilate alanine as a sole source of carbon and energy. agarose assimilation assay Carrine Blank agarose assimilation agarose Assays for the ability of a microorganism to assimilate agarose as a sole source of carbon and energy. agar assimilation assay agar Assays for the ability of a microorganism to assimilate agar as a sole source of carbon and energy. agar assimilation Carrine Blank phenylethylamine assimilation assay Carrine Blank phenylethylamine assimilation phenylethylamine Assays for the ability of a microorganism to assimilate phenylethylamine as a sole source of carbon and energy. adenine assimilation assay adenine adenine assimilation Carrine Blank Assays for the ability of a microorganism to assimilate adenine as a sole source of carbon and energy. acrylic acid assimilation assay Assays for the ability of a microorganism to assimilate acrylate (acrylic acid) as a sole source of carbon and energy. acrylate acrylate assimilation acrylic acid Carrine Blank acetone assimilation assay Assays for the ability of a microorganism to assimilate acetone as a sole source of carbon and energy. Carrine Blank acetone assimilation acetone acetoin assimilation assay acetoin assimilation Assays for the ability of a microorganism to assimilate acetoin as a sole source of carbon and energy. acetoin Carrine Blank acetamide assimilation assay acetamide Assays for the ability of a microorganism to assimilate acetamide as a sole source of carbon and energy. Carrine Blank acetamide assimilation 6-O-acetyl-D-glucose assimilation assay 6-O-acetyl-D-glucose N-acetyl-glucoside Assays for the ability of a microorganism to assimilate 6-O-acetyl-D-glucose as a sole source of carbon and energy. N-acetyl-glucose N-acetyl-glucopyranoside Carrine Blank 6-O-acetyl-D-glucose assimilation xylooligosaccharide assimilation assay xylooligosaccharides Carrine Blank xylooligosaccharide xylooligosaccharide assimilation Assays for the ability of a microorganism to assimilate xylooligosaccharide as a sole source of carbon and energy. 5-aminopentanoic acid assimilation assay 5-aminovalerate Assays for the ability of a microorganism to assimilate 5-aminopentanoate (5-aminopentanoic acid) as a sole source of carbon and energy. 5-aminopentanoate 5-aminopentanoate assimilation 5-aminopentanoic acid Carrine Blank 4-oxopentanoic acid assimilation assay gamma-ketovalerate Assays for the ability of a microorganism to assimilate 4-oxopentanoate (4-oxopentanoic acid) as a sole source of carbon and energy. 4-ketovalerate g-ketovalerate 4-oxopentanoate assimilation gamma-ketovaleric acid Carrine Blank 4-oxopentanoic acid 4-oxopentanoate 4-methoxycinnamic acid assimilation assay 4-methoxycinnamic acid methoxycinnamate Assays for the ability of a microorganism to assimilate 4-methoxycinnamic acid as a sole source of carbon and energy. Carrine Blank methoxycinnamic acid 4-methoxycinnamic acid assimilation carboxymethylcellulose assimilation assay carboxymethyl cellulose carboxymethylcellulose assimilation Assays for the ability of a microorganism to assimilate carboxymethylcellulose as a sole source of carbon and energy. carboymethyl cellulose carboxymethylcellulose cm-cellulose Carrine Blank catechol assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate catechol as a sole source of carbon and energy. catechol assimilation catechol cellobiose assimilation assay Assays for the ability of a microorganism to assimilate cellobiose as a sole source of carbon and energy. cellobiose Carrine Blank cellobiose assimilation cellibiose chitin assimilation assay chitin assimilation chitin Carrine Blank Assays for the ability of a microorganism to assimilate chitin as a sole source of carbon and energy. crab shell chitosan assimilation assay chitosan assimilation Assays for the ability of a microorganism to assimilate chitosan as a sole source of carbon and energy. chitosan Carrine Blank cholesterol assimilation assay cholesterol assimilation cholesterol Assays for the ability of a microorganism to assimilate cholesterol as a sole source of carbon and energy. Carrine Blank choline assimilation assay choline chloride choline Assays for the ability of a microorganism to assimilate choline as a sole source of carbon and energy. Carrine Blank choline assimilation cinnamic acid assimilation assay cinnamate assimilation cinnamic acid Assays for the ability of a microorganism to assimilate cinnamate (cinnamic acid) as a sole source of carbon and energy. cinnamate Carrine Blank cinnamyl alcohol assimilation assay Carrine Blank cinnamyl alcohol assimilation Assays for the ability of a microorganism to assimilate cinnamyl alcohol as a sole source of carbon and energy. cinnamyl alcohol citrulline assimilation assay Carrine Blank citrulline assimilation citrulline Assays for the ability of a microorganism to assimilate citrulline as a sole source of carbon and energy. coniferol assimilation assay coniferol assimilation Assays for the ability of a microorganism to assimilate coniferol as a sole source of carbon and energy. Carrine Blank coniferol 4-hydroxy-3-methoxycinnamyl alcohol 4-hydroxy-3-methoxycinnamylalcohol coumaric acid assimilation assay coumarate assimilation Assays for the ability of a microorganism to assimilate coumarate (coumaric acid) as a sole source of carbon and energy. Carrine Blank coumaric acid coumarate creatine assimilation assay creatine assimilation creatine Carrine Blank Assays for the ability of a microorganism to assimilate creatine as a sole source of carbon and energy. creatinine assimilation assay Carrine Blank creatinine assimilation creatinine L-creatinine Assays for the ability of a microorganism to assimilate creatinine as a sole source of carbon and energy. crotonic acid assimilation assay Carrine Blank crotonate assimilation crotonate Assays for the ability of a microorganism to assimilate crotonate (crotonic acid) as a sole source of carbon and energy. crotonic acid crystalline cellulose assimilation assay cardboard boric acid-treated cellulose D-cellulos cellulose vegetable cellulose preparation soluble cellulose whatman filter paper crystalline cellulose assimilation cellulose powder Carrine Blank crystalline cellulose Assays for the ability of a microorganism to assimilate crystalline cellulose as a sole source of carbon and energy. fp cellulose filter paper microcrystalline cellulose (1->3)-beta-D-glucan assimilation assay (1->3)-beta-D-glucan assimilation curdlan Assays for the ability of a microorganism to assimilate (1->3)-beta-D-glucan as a sole source of carbon and energy. (1->3)-beta-D-glucan Carrine Blank cyclodextrin assimilation assay Carrine Blank cyclodextrin assimilation cyclodextrine Assays for the ability of a microorganism to assimilate cyclodextrin as a sole source of carbon and energy. cyclodextrins cyclo-dextrin cyclodextrin cyclohexanecarboxylic acid assimilation assay cyclohexanecarboxylic acid Assays for the ability of a microorganism to assimilate cyclohexanecarboxylate (cyclohexanecarboxylic acid) as a sole source of carbon and energy. cyclohexanecarboxylate cyclohexanecarboxylate assimilation Carrine Blank cyclohexanol assimilation assay Carrine Blank cyclohexanol assimilation Assays for the ability of a microorganism to assimilate cyclohexanol as a sole source of carbon and energy. hexanol cyclohexanol cysteine assimilation assay cysteine assimilation Assays for the ability of a microorganism to assimilate cysteine as a sole source of carbon and energy. Carrine Blank cysteine DL-cysteine L-cysteine assimilation assay Assays for the ability of a microorganism to assimilate L-cysteine as a sole source of carbon and energy. L-cysteine-HCl Carrine Blank L-cysteine assimilation L-cysteine cystine assimilation assay Carrine Blank cystine Assays for the ability of a microorganism to assimilate cystine as a sole source of carbon and energy. cystine assimilation L-cystine assimilation assay L-cystine assimilation Assays for the ability of a microorganism to assimilate L-cystine as a sole source of carbon and energy. L-cystine Carrine Blank dodecanoic acid assimilation assay dodecanoate assimilation Carrine Blank dodecanoate dodecanoic acid Assays for the ability of a microorganism to assimilate dodecanoate (dodecanoic acid) as a sole source of carbon and energy. Carrine Blank dimethylamine assimilation assay dimethylamine assimilation Assays for the ability of a microorganism to assimilate dimethylamine as a sole source of carbon and energy. dimethylamine Carrine Blank dimethyl sulfoxide assimilation assay dimethyl sulfoxide assimilation DMSO Carrine Blank dimethyl sulfoxide Assays for the ability of a microorganism to assimilate dimethyl sulfoxide as a sole source of carbon and energy. dimethyl sulfide assimilation assay dimethyl sulfide assimilation Assays for the ability of a microorganism to assimilate dimethyl sulfide as a sole source of carbon and energy. Carrine Blank dimethyl sulfide dimethylsulfide DMS dihydroxyacetone assimilation assay dihydroxyacetone assimilation dihydroxyacetone Assays for the ability of a microorganism to assimilate dihydroxyacetone as a sole source of carbon and energy. Carrine Blank dextran assimilation assay dextran assimilation Carrine Blank dextran Assays for the ability of a microorganism to assimilate dextran as a sole source of carbon and energy. deoxyribonucleic acid assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate deoxyribonucleic acid as a sole source of carbon and energy. deoxyribonucleic acid assimilation deoxyribonucleic acid DNA D-glucopyranose 6-phosphate assimilation assay D-glucopyranose 6-phosphate Assays for the ability of a microorganism to assimilate D-glucopyranose 6-phosphate as a sole source of carbon and energy. Carrine Blank D-glucopyranose 6-phosphate assimilation D-glucopyranose 1-phosphate assimilation assay D-glucose 1-PO4 glucose 1-phosphate D-glucopyranose 1-phosphate assimilation Assays for the ability of a microorganism to assimilate D-glucopyranose 1-phosphate as a sole source of carbon and energy. glucose 1-PO4 glucose 1-phosphate assimilation glucose 1-PO4 assimilation D-glucose 1-phosphate D-glucopyranose 1-phosphate Carrine Blank cytosine assimilation assay Carrine Blank cytosine assimilation Assays for the ability of a microorganism to assimilate cytosine as a sole source of carbon and energy. cytosine cyclohexanecarboxylic acid fermentation assay cyclohexanecarboxylate fermentation cyclohexanecarboxylate acidification Assays for the ability of a microorganism to ferment cyclohexanecarboxylate (cyclohexanecarboxylic acid). cyclohexanecarboxylate Carrine Blank cyclohexanecarboxylic acid cyclohexanol fermentation assay cyclohexanol acidification Assays for the ability of a microorganism to ferment cyclohexanol. cyclohexanol Carrine Blank cyclohexanol fermentation hexanol cysteine fermentation assay Assays for the ability of a microorganism to ferment cysteine. cysteine cysteine fermentation DL-cysteine cysteine acidification Carrine Blank L-cysteine fermentation assay L-cysteine fermentation L-cysteine L-cysteine-HCl Carrine Blank L-cysteine acidification Assays for the ability of a microorganism to ferment L-cysteine. cystine fermentation assay Assays for the ability of a microorganism to ferment cystine. cystine acidification cystine cystine fermentation Carrine Blank L-cystine fermentation assay Carrine Blank L-cystine fermentation L-cystine L-cystine acidification Assays for the ability of a microorganism to ferment L-cystine. alpha-D-glucose 1-phosphate fermentation assay alpha-D-glucose 1-phosphate fermentation Assays for the ability of a microorganism to ferment alpha-D-glucose 1-phosphate. Carrine Blank a-D-glucose 1-phosphate alpha-D-glucose 1-phosphate alpha-D-glucose 1-phosphate acidification a-D-glucose 1-PO4 (1->3)-beta-D-glucan fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment (1->3)-beta-D-glucan. curdlan (1->3)-beta-D-glucan (1->3)-beta-D-glucan acidification (1->3)-beta-D-glucan fermentation crystalline cellulose fermentation assay fp cellulose D-cellulose Assays for the ability of a microorganism to ferment crystalline cellulose. Carrine Blank filter paper vegetable cellulose preparation microcrystalline cellulose whatman filter paper cardboard crystalline cellulose cellulose cellulose powder soluble cellulose boric acid-treated cellulose crystalline cellulose acidification crystalline cellulose fermentation crotonic acid fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment crotonate (crotonic acid). crotonate acidification crotonic acid crotonate fermentation crotonate creatine fermentation assay creatine fermentation creatine acidification Carrine Blank Assays for the ability of a microorganism to ferment creatine. creatine coniferol fermentation assay Carrine Blank 4-hydroxy-3-methoxycinnamylalcohol Assays for the ability of a microorganism to ferment coniferol. 4-hydroxy-3-methoxycinnamyl alcohol coniferol acidification coniferol fermentation coniferol citrulline fermentation assay citrulline Carrine Blank Assays for the ability of a microorganism to ferment citrulline. citrulline acidification citrulline fermentation cis-aconitic acid fermentation assay cis-aconitic acid fermentation Assays for the ability of a microorganism to ferment cis-aconitic acid (cis-aconitate). cisaconitate cis-aconitate cis-aconitic acid cis-aconitic acid acidification Carrine Blank cinnamyl alcohol fermentation assay cinnamyl alcohol Carrine Blank cinnamyl alcohol fermentation Assays for the ability of a microorganism to ferment cinnamyl alcohol. cinnamyl alcohol acidification cinnamic acid fermentation assay cinnamate fermentation cinnamate acidification cinnamic acid cinnamate Carrine Blank Assays for the ability of a microorganism to ferment cinnamate (cinnamic acid). choline fermentation assay choline fermentation choline Carrine Blank choline chloride choline acidification Assays for the ability of a microorganism to ferment choline. cholesterol fermentation assay cholesterol fermentation Assays for the ability of a microorganism to ferment cholesterol. cholesterol acidification Carrine Blank cholesterol chitosan fermentation assay Assays for the ability of a microorganism to ferment chitosan. Carrine Blank chitosan chitosan fermentation chitosan acidification chitin fermentation assay Assays for the ability of a microorganism to ferment chitin. chitin fermentation chitin crab shell chitin acidification Carrine Blank L-alanine fermentation assay L-alanine fermentation L-alanine acidification Assays for the ability of a microorganism to ferment L-alanine. L-alanine Carrine Blank L-aspartic acid fermentation assay L-aspartic acid acidification L-aspartic acid sodium L-aspartate L-aspartate L-asparatate Assays for the ability of a microorganism to ferment L-aspartic acid (L-aspartate). Carrine Blank L-aspartic acid fermentation D-aspartic acid fermentation assay D-aspartic acid fermentation Assays for the ability of a microorganism to ferment D-aspartic acid (D-aspartate). Carrine Blank D-aspartic acid D-aspartic acid acidification D-aspartate D-alanine fermentation assay Carrine Blank D-alanine D-alanine fermentation Assays for the ability of a microorganism to ferment D-alanine. D-alanine acidification D-glucopyranose 6-phosphate fermentation assay D-glucopyranose 6-phosphate fermentation Carrine Blank Assays for the ability of a microorganism to ferment D-glucopyranose 6-phosphate. D-glucopyranose 6-phosphate acidification D-glucopyranose 6-phosphate cellobiose fermentation assay Carrine Blank cellibiose cellobiose fermentation Assays for the ability of a microorganism to ferment cellobiose. CEL cellobiose cellobiose acidification catechol fermentation assay catechol fermentation Assays for the ability of a microorganism to ferment catechol. catechol Carrine Blank catechol acidification carnitine fermentation assay carnitine D,L-carnitine Carrine Blank Assays for the ability of a microorganism to ferment carnitine. carnitine fermentation DL-carnitine carnitine acidification carboxymethylcellulose fermentation assay Carrine Blank carboxymethyl cellulose cm-cellulose carboxymethylcellulose acidification Assays for the ability of a microorganism to ferment carboxymethylcellulose. carboxymethylcellulose carboxymethylcellulose fermentation carboymethyl cellulose caffeic acid fermentation assay Assays for the ability of a microorganism to ferment caffeic acid (cis-caffeate or trans-caffeate). trans-caffeate caffeic acid cis-caffeate caffeic acid fermentation Carrine Blank caffeic acid acidification cadaverine fermentation assay Assays for the ability of a microorganism to ferment cadaverine. cadaverine fermentation Carrine Blank cadaverine acidification cadaverine butyric acid fermentation assay n-butyrate Assays for the ability of a microorganism to ferment butyrate (butyric acid). n-butyric acid butyrate butyric acid butyrate fermentation n-butyric butanoic acid Carrine Blank butyrate acidification butanone fermentation assay butanone fermentation butanone Carrine Blank butanone acidification Assays for the ability of a microorganism to ferment butanone. butane-2,3-dione fermentation assay Assays for the ability of a microorganism to ferment butane-2,3-dione. diacetyl butane-2,3-dione fermentation butane-2,3-dione butane-2,3-dione acidification 2,3-butanedione Carrine Blank butane-2,3-diol fermentation assay 2,3-buthanediol 2,3-butanediol butane-2,3-diol butane-2,3-diol fermentation Carrine Blank Assays for the ability of a microorganism to ferment butane-2,3-diol. butane-2,3-diol acidification butane-1,3-diol fermentation assay butane-1,3-diol fermentation butane-1,3-diol butane-1,3-diol acidification Assays for the ability of a microorganism to ferment butane-1,3-diol. 1,3-butanediol Carrine Blank gellan gum fermentation assay xanthan gum gellan gum acidification Assays for the ability of a microorganism to ferment gellan gum. Carrine Blank gellan gum fermentation gellan gum gelatin fermentation assay Carrine Blank gelatine gelatin Assays for the ability of a microorganism to ferment gelatin. gelatin fermentation gelatin acidification gallic acid fermentation assay Assays for the ability of a microorganism to ferment gallate (gallic acid). gallate acidification gallate Carrine Blank gallate fermentation gallic acid alpha-D-galacturonic acid fermentation assay a-D-galacturonic acid a-D-galacturonate Assays for the ability of a microorganism to ferment alpha-D-galacturonate (D-galacturonic acid). Carrine Blank alpha-D-galacturonate alpha-D-galacturonate acidification alpha-D-galacturonate fermentation D-galacturonic acid galacturonic acid fermentation assay Assays for the ability of a microorganism to ferment galacturonate (galacturonic acid). galactwonate galacturonate galacturonate fermentation galacturonate acidification galacturonic acid Carrine Blank L-lyxose fermentation assay L-lyxose fermentation L-lyxose Carrine Blank L-lyxose acidification Assays for the ability of a microorganism to ferment L-lyxose. galactose fermentation assay galactose fermentation galactose Assays for the ability of a microorganism to ferment galactose. Carrine Blank galactose acidification L-galactono-1,4-lactone fermentation assay galactono-1,4-lactone L-galactono-1,4-lactone acidification Assays for the ability of a microorganism to ferment L-galactono-1,4-lactone. Carrine Blank L-galacturonic acid lactone L-galactono-1,4-lactone fermentation L-galactono-1,4-lactone galactonolactone fermentation assay Assays for the ability of a microorganism to ferment galactonolactone. Carrine Blank galactonolactone acidification galactonolactone fermentation galactonolactone galactaric acid fermentation assay galactaric acid mucate galactaric acid fermentation galactarate mucic acid Assays for the ability of a microorganism to ferment galactaric acid (galactarate). Carrine Blank galactaric acid acidification gamma-cyclodextrin fermentation assay gamma-cyclodextrin fermentation gamma-cyclodextrin acidification Carrine Blank Assays for the ability of a microorganism to ferment gamma-cyclodextrin. gamma-cyclodextrin gamma-aminobutyric acid fermentation assay gamma-aminobutyrate Assays for the ability of a microorganism to ferment gamma-aminobutyrate (gamma-aminobutyric acid). Carrine Blank gamma-aminobutyrate fermentation g-aminobutryric acid g-aminobutyric acid gamma-aminobutyric acid gamma-aminobutyrate acidification 4-aminobutyric acid fusidic acid fermentation assay fusidic acid fermentation Carrine Blank fusidic acid Assays for the ability of a microorganism to ferment fusidic acid (fusidate). fusidate fusidic acid acidification fumaric acid fermentation assay fumaric acid fumarate Carrine Blank sodium fumarate fumaric acid fermentation Assays for the ability of a microorganism to ferment fumaric acid (fumarate). fumaric acid acidification (R)-lactic acid fermentation assay D-lactic acid Dlactic acid (R)-lactate fermentation Carrine Blank (R)-lactate (R)-lactate acidification D-lactate Assays for the ability of a microorganism to ferment (R)-lactate. isovanillin fermentation assay Carrine Blank 3-hydroxy-4-methoxybenzaldehyde isovanillin isovanillin fermentation Assays for the ability of a microorganism to ferment isovanillin. isovanillin acidification fucose fermentation assay D- or L-fucose Assays for the ability of a microorganism to ferment fucose. fucose fermentation fucose acidification Carrine Blank DL-fucose D- and L-fucose fucose D-fructose 6-phosphate fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment D-fructose 6-phosphate. D-fructose 6-phosphate acidification D-fructose 6-PO4 D-fructose 6-phosphate D-fructose 6-phosphate fermentation fructose 6-phosphate fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment fructose 6-phosphate. fructose 6-PO4 fructose 6-phosphate acidification fructose 6-phosphate fructose 6-phosphate fermentation fructose fermentation assay Assays for the ability of a microorganism to ferment fructose. fructose acidification FRU fructose fermentation levulose Carrine Blank fructose formic acid fermentation assay formic acid Carrine Blank formate formate acidification Assays for the ability of a microorganism to ferment formate (formic acid). formate fermentation sodium formate ferulic acid fermentation assay ferulate 4-hydroxy-3-methoxycinnamate ferulate acidification Carrine Blank ferulate fermentation Assays for the ability of a microorganism to ferment ferulate (ferulic acid). ferulic acid ethylene glycol fermentation assay ethylene glycol ethylene glycol acidification Assays for the ability of a microorganism to ferment ethylene glycol. Carrine Blank ethyleneglycol ethylene glycol fermentation ethanolamine fermentation assay ethanolamine ethanolamine acidification Carrine Blank ethanolamine fermentation Assays for the ability of a microorganism to ferment ethanolamine. ethanol fermentation assay ethyl alcohol Carrine Blank ethanol fermentation ethanol ethanol acidification Assays for the ability of a microorganism to ferment ethanol. erythrose fermentation assay Assays for the ability of a microorganism to ferment erythrose. Carrine Blank erythrose fermentation erythrose acidification erythrose dodecanoic acid fermentation assay dodecanoate fermentation dodecanoate acidification dodecanoic acid dodecanoate Carrine Blank Assays for the ability of a microorganism to ferment dodecanoate (dodecanoic acid). beta-galactosidase with resorufin Carrine Blank An assay for the activity of beta-galactosidase in a microorganism. Uses the substrate resorufin-beta-D-galactopyranoside. Beta-galactosidase will cleave the substrate, producing resorufin, which is pink. A positive result is fluorescent pink/red-orange; a negative result is orange. bGAR dimethylamine fermentation assay Carrine Blank dimethylamine fermentation Assays for the ability of a microorganism to ferment dimethylamine. dimethylamine acidification dimethylamine dimethyl sulfoxide fermentation assay Carrine Blank dimethyl sulfoxide fermentation dimethyl sulfoxide DMSO dimethyl sulfoxide acidification Assays for the ability of a microorganism to ferment dimethyl sulfoxide. dimethyl sulfide fermentation assay DMS dimethyl sulfide fermentation dimethyl sulfide Carrine Blank dimethylsulfide dimethyl sulfide acidification Assays for the ability of a microorganism to ferment dimethyl sulfide. dihydroxyacetone fermentation assay dihydroxyacetone dihydroxyacetone acidification dihydroxyacetone fermentation Assays for the ability of a microorganism to ferment dihydroxyacetone. Carrine Blank dextrin fermentation assay dexrin Assays for the ability of a microorganism to ferment dextrin. dextrin acidification dextrin dextrin fermentation dex trin dextrin crystals Carrine Blank dextran fermentation assay dextran acidification Carrine Blank dextran dextran fermentation Assays for the ability of a microorganism to ferment dextran. deoxyribonucleic acid fermentation assay deoxyribonucleic acid acidification deoxyribonucleic acid fermentation Carrine Blank deoxyribonucleic acid Assays for the ability of a microorganism to ferment deoxyribonucleic acid. DNA decanoic acid fermentation assay decanoate acidification Carrine Blank decanoic acid decanoate fermentation capric acid caprate n-capric acid Assays for the ability of a microorganism to ferment decanoate (decanoic acid). decanoate D-glucose 6-phosphate fermentation assay D-glucose 6-PO4 D-glucose 6-phosphate acidification glucose 6-phosphate Carrine Blank glucose 6-PO4 D-glucose 6-phosphate Assays for the ability of a microorganism to ferment D-glucose 6-phosphate. D-glucose 6-phosphate fermentation D-glucopyranose 1-phosphate fermentation assay D-glucopyranose 1-phosphate fermentation glucose 1-phosphate glucose 1-PO4 Carrine Blank Assays for the ability of a microorganism to ferment D-glucopyranose 1-phosphate. D-glucopyranose 1-phosphate acidification D-glucose 1-PO4 glucose 1-phospate D-glucose 1-phosphate D-glucopyranose 1-phosphate cytosine fermentation assay cytosine fermentation Carrine Blank cytosine acidification Assays for the ability of a microorganism to ferment cytosine. cytosine erythrose assimilation assay erythrose assimilation erythrose Assays for the ability of a microorganism to assimilate erythrose as a sole source of carbon and energy. Carrine Blank esculin assimilation assay Assays for the ability of a microorganism to assimilate esculin as a sole source of carbon and energy. esculine esculin ferric citrate esculin assimilation Carrine Blank aesculin ferric citrate esculin aesculin assimilation aesculin ethanol assimilation assay Carrine Blank ethanol assimilation Assays for the ability of a microorganism to assimilate ethanol as a sole source of carbon and energy. ethanol ethyl alcohol ethylene glycol assimilation assay Assays for the ability of a microorganism to assimilate ethylene glycol as a sole source of carbon and energy. ethyleneglycol Carrine Blank ethylene glycol assimilation ethylene glycol ferulic acid assimilation assay ferulate assimilation Assays for the ability of a microorganism to assimilate ferulate (ferulic acid) as a sole source of carbon and energy. ferulic acid Carrine Blank 4-hydroxy-3-methoxycinnamate ferulate fructose assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate fructose as a sole source of carbon and energy. fructose assimilation fructose fructose 6-phosphate assimilation assay fructose 6-phosphate fructose 6-phosphate assimilation Assays for the ability of a microorganism to assimilate fructose 6-phosphate as a sole source of carbon and energy. Carrine Blank fructose 6-PO4 fucose assimilation assay fucose Assays for the ability of a microorganism to assimilate fucose as a sole source of carbon and energy. Carrine Blank DL-fucose FUC D- or L-fucose D- and L-fucose fucose assimilation fusidic acid assimilation assay fusidic acid fusidate Assays for the ability of a microorganism to assimilate fusidic acid (fusidate) as a sole source of carbon and energy. fusidic acid assimilation Carrine Blank gamma-cyclodextrin assimilation assay gamma-cyclodextrin assimilation Assays for the ability of a microorganism to assimilate gamma-cyclodextrin as a sole source of carbon and energy. Carrine Blank gamma-cyclodextrin galactonolactone assimilation assay galactonate lactone Carrine Blank L-galactonate lactone galactonic acid lactone galactonic acid lactone assimilation L-galactonic acid lactone assimilation L-galactonate lactone assimilation galactonate lactone assimilation Assays for the ability of a microorganism to assimilate galactonolactone as a sole source of carbon and energy. L-galactonic acid lactone galactonolactone assimilation galactonolactone galactose assimilation assay Assays for the ability of a microorganism to assimilate galactose as a sole source of carbon and energy. galactose galactose assimilation Carrine Blank galacturonic acid assimilation assay galacturonic acid assimilation galacturonate assimilation galacturonate galacturonic acid Assays for the ability of a microorganism to assimilate galacturonate (galacturonic acid) as a sole source of carbon and energy. Carrine Blank galactwonate gallic acid assimilation assay gallic acid Assays for the ability of a microorganism to assimilate gallate (gallic acid) as a sole source of carbon and energy. gallate Carrine Blank gallate assimilation gellan gum assimilation assay gellan gum Assays for the ability of a microorganism to assimilate gellan gum as a sole source of carbon and energy. Carrine Blank xanthan gum gellan gum assimilation Glu-Glu assimilation assay glutamyl glutamate Assays for the ability of a microorganism to assimilate Glu-Glu as a sole source of carbon and energy. Glu-Glu Glu-Glu assimilation glutamyl L-glutamic acid glutamyl-glutamic acid Carrine Blank glucaric acid assimilation assay saccharic acid glucarate assimilation glucarate Assays for the ability of a microorganism to assimilate glucaric acid (glucarate) as a sole source of carbon and energy. Carrine Blank saccharate glucaric acid glucaric acid assimilation glucitol assimilation assay glucitol SOR sorbitol glucitol assimilation Carrine Blank sorbitol assimilation Assays for the ability of a microorganism to assimilate glucitol as a sole source of carbon and energy. gluconic acid assimilation assay Carrine Blank gluconic acid potassium gluconate assimilation sodium gluconate Assays for the ability of a microorganism to assimilate gluconate (gluconic acid) as a sole source of carbon and energy. GNT gluconic acid sodium salt potassium gluconate gluconate gluconate assimilation gluconic acid assimilation glucosamine assimilation assay glucosamine hydrochloride Carrine Blank glucosamine glucosamine assimilation Assays for the ability of a microorganism to assimilate glucosamine as a sole source of carbon and energy. glucose assimilation assay Carrine Blank glucose GLU Assays for the ability of a microorganism to assimilate glucose as a sole source of carbon and energy. glucose assimilation rac-methyl lactate assimilation assay Assays for the ability of a microorganism to assimilate rac-methyl lactate as a sole source of carbon and energy. lactic acid methyl ester assimilation methyl lactate lactate methyl ester assimilation rac-methyl lactate assimilation Carrine Blank lactic acid methyl ester lactate methyl ester rac-methyl lactate D-lactic acid methyl ester N-acetylglucosamine assimilation assay N-acetylglucosoamine NAG NAG NAGa N-acetylglucosamine assimilation Assays for the ability of a microorganism to assimilate N-acetylglucosamine as a sole source of carbon and energy. NAGa acetylglucosamine Carrine Blank Nacetylglucosamine Nacetyl-glucosamine N-acetylglycosamine N-acetylglucosamine N-acetyl-glucosamine methylamine assimilation assay Assays for the ability of a microorganism to assimilate methylamine as a sole source of carbon and energy. methylamine Carrine Blank methylamine assimilation monomethylamine alpha-D-glucose 6-phosphate fermentation assay alpha-D-glucose 6-phosphate fermentation Assays for the ability of a microorganism to ferment alpha-D-glucose 6-phosphate. a-D-glucose 6-phosphate Carrine Blank alpha-D-glucose 6-phosphate alpha-D-glucose 6-phosphate acidification a-D-glucose 6-PO4 glucoside fermentation assay glucoside glucoside acidification glucoside fermentation glucopyranoside D-glucopyranoside D-glucoside Assays for the ability of a microorganism to ferment glucoside. Carrine Blank glucosiduronic acid fermentation assay beta-D-glucosiduronate Carrine Blank glucosiduronic acid glucuronide glucosiduronic acid acidification glucosiduronate Assays for the ability of a microorganism to ferment glucosiduronic acid (alpha-D-glucosiduronate or beta-D-glucosiduronate). alpha-D-glucosiduronate glucosiduronic acid fermentation glucuronic acid fermentation assay glucuronate fermentation glucuronate acidification glucuronic acid Assays for the ability of a microorganism to ferment glucuronate (glucuronic acid). glucuronate Carrine Blank D-glucuronic acid fermentation assay D-glucuronic acid Carrine Blank D-glucuronate acidification Assays for the ability of a microorganism to ferment D-glucuronate (D-glucuronic acid). D-glucuronate fermentation D-glucuronate glutamic acid fermentation assay glutamic acid acidification glutamate Assays for the ability of a microorganism to ferment glutamic acid (glutamate). sodium glutamate glutatmate glutamic acid glutamic acid fermentation Carrine Blank L-glutamic acid fermentation assay Carrine Blank L-glutamic acid fermentation L-glutamate L-glutamic acid sodium L-glutamate L-glutamic acid acidification Assays for the ability of a microorganism to ferment L-glutamic acid (L-glutamate). gamma-poly(L-glutamic acid) fermentation assay Assays for the ability of a microorganism to ferment gamma-poly(L-glutamic acid). gamma-poly(L-glutamic acid) acidification Carrine Blank gamma-poly(L-glutamic acid) gamma-poly(L-glutamic acid) fermentation glutamine fermentation assay glutamine fermentation Assays for the ability of a microorganism to ferment glutamine. glutamine acidification Carrine Blank glutamine L-glutamine fermentation assay Assays for the ability of a microorganism to ferment L-glutamine. L-glutamine L-glutamine fermentation L-glutamine acidification Carrine Blank glutaric acid fermentation assay Assays for the ability of a microorganism to ferment glutarate (glutaric acid). glutarate glutarate fermentation glutarate acidification glutaric acid Carrine Blank glutathione fermentation assay glutathione acidification Carrine Blank glutathione fermentation glutathione Assays for the ability of a microorganism to ferment glutathione. Gly-Asp fermentation assay glycyl-L-aspartate glycyl-L-aspartic acid Gly-Asp acidification Gly-Asp glycyl aspartate Assays for the ability of a microorganism to ferment Gly-Asp. Gly-Asp fermentation Carrine Blank Gly-Glu fermentation assay Gly-Glu Assays for the ability of a microorganism to ferment Gly-Glu. Gly-Glu fermentation glycyl L-glutamate glycyl L-glutamic acid Carrine Blank Gly-Glu acidification glycyl glutamate Gly-Pro fermentation assay glycyl-L-prolin Gly-Pro fermentation Carrine Blank Gly-Pro Gly-Pro acidification glycyl proline Assays for the ability of a microorganism to ferment Gly-Pro. glyceric acid fermentation assay DL-glycerate Assays for the ability of a microorganism to ferment glycerate (glyceric acid). Carrine Blank glycerate acidification glycerate fermentation glyceric acid glycerate glycerol 1-phosphate fermentation assay Assays for the ability of a microorganism to ferment glycerol 1-phosphate. glycerol 1-phosphate acidification glycerol 1-phosphate fermentation glycerol 1-phosphate Carrine Blank glycine fermentation assay glycine acidification glycine fermentation Carrine Blank glycine Assays for the ability of a microorganism to ferment glycine. L-glycine glycine betaine fermentation assay glycine betaine Carrine Blank glycine betaine acidification glycine betaine fermentation Assays for the ability of a microorganism to ferment glycine betaine. glycolaldehyde fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment glycolaldehyde. glycolaldehyde glycolaldehyde fermentation glycolaldehyde acidification glycolic acid fermentation assay glycolate acidification Carrine Blank Assays for the ability of a microorganism to ferment glycolate (glycolic acid). glycolate fermentation glycolate glycolic acid Gly-Gln fermentation assay Assays for the ability of a microorganism to ferment Gly-Gln. glycyl glutamine Gly-Gln Gly-Gln fermentation Carrine Blank Gly-Gln acidification Gly-Met fermentation assay glycyl methionine Assays for the ability of a microorganism to ferment Gly-Met. Carrine Blank Gly-Met acidification Gly-Met fermentation Gly-Met glycyl-glycyl-glycine fermentation assay Gly-Gly-Gly Carrine Blank glycyl-glycyl-glycine acidification glycyl-glycyl-glycine fermentation Assays for the ability of a microorganism to ferment glycyl-glycyl-glycine. glycyl-glycyl-glycine glycylglycine fermentation assay Gly-Gly glycylglycine fermentation Assays for the ability of a microorganism to ferment glycylglycine. Carrine Blank glycylglycine glycylglycine acidification glyoxylic acid fermentation assay glyoxylate fermentation glyoxylic acid glyoxylate acidification Carrine Blank glyoxylate Assays for the ability of a microorganism to ferment glyoxylate (glyoxylic acid). guanidine fermentation assay Carrine Blank guanidine guanidine acidification guanidine fermentation Assays for the ability of a microorganism to ferment guanidine. guanine fermentation assay guanine fermentation Carrine Blank guanine guanine acidification Assays for the ability of a microorganism to ferment guanine. hemicellulose fermentation assay hemicellulose fermentation hemicellulose hemicellulose acidification Carrine Blank Assays for the ability of a microorganism to ferment hemicellulose. hexadecanoic acid fermentation assay palmitic acid hexadecanoic acid hexadecanoate hexadecanoate acidification palmitic straight-chain sautrated palmitic acid Assays for the ability of a microorganism to ferment hexadecanoate (hexadecanoic acid). hexadecanoate fermentation Carrine Blank palmitate trans-caffeic acid fermentation assay trans-caffeate fermentation Assays for the ability of a microorganism to ferment trans-caffeate. Carrine Blank trans-caffeate acidification trans-caffeate glucose fermentation assay glucose GLU fermentation/glucose Carrine Blank glucose fermentation glucose acidification Assays for the ability of a microorganism to ferment glucose. glucosamine fermentation assay glucosamine hydrochloride Assays for the ability of a microorganism to ferment glucosamine. glucosamine acidification glucosamine Carrine Blank glucosamine fermentation D-gluconic acid fermentation assay D-gluconic acid D-gluconate acidification Carrine Blank D-gluconate fermentation Assays for the ability of a microorganism to ferment D-gluconate (D-gluconic acid). D-gluconate cis-caffeic acid fermentation assay Assays for the ability of a microorganism to ferment cis-caffeate. cis-caffeate acidification cis-caffeate fermentation cis-caffeate Carrine Blank glucitol fermentation assay glucitol sorbitol Carrine Blank glucitol fermentation sorbitol acidification sorbitol fermentation Assays for the ability of a microorganism to ferment glucitol. SBL glucitol acidification SOR D-glucaric acid fermentation assay D-glucaric acid fermentation Carrine Blank D-saccharate D-glucarate D-glucaric acid acidification D-saccharic acid Assays for the ability of a microorganism to ferment D-glucaric acid (D-glucarate). D-glucaric acid glucaric acid fermentation assay Carrine Blank glucarate glucaric acid saccharate glucaric acid acidification saccharic acid Assays for the ability of a microorganism to ferment glucaric acid (glucarate). glucaric acid fermentation Glu-Glu fermentation assay glutamyl-glutamic acid Glu-Glu glutamyl glutamate glutamyl L-glutamic acid Glu-Glu acidification Assays for the ability of a microorganism to ferment Glu-Glu. Glu-Glu fermentation Carrine Blank propane-1,3-diol assimilation assay Carrine Blank propane-1,3-diol 1,3-propanediol Assays for the ability of a microorganism to assimilate propane-1,3-diol as a sole source of carbon and energy. propane-1,3-diol assimilation propane-1,2-diol assimilation assay Assays for the ability of a microorganism to assimilate propane-1,2-diol as a sole source of carbon and energy. propane-1,2-diol assimilation 1,2-propanediol Carrine Blank propane-1,2-diol 1,2-propandiol propyleneglycol propylene glycol propan-2-ol assimilation assay propan-2-ol Assays for the ability of a microorganism to assimilate propan-2-ol as a sole source of carbon and energy. Carrine Blank 2-propanol iso-propanol i-propanol isopropanol propan-2-ol assimilation propan-1-ol assimilation assay propan-1-ol propan-1-ol assimilation 1-propanol propanol Assays for the ability of a microorganism to assimilate propan-1-ol as a sole source of carbon and energy. Carrine Blank n-propanol proline assimilation assay PRO proline Assays for the ability of a microorganism to assimilate proline as a sole source of carbon and energy. Carrine Blank proline assimilation polysorbate 60 assimilation assay Carrine Blank Tween 60 assimilation Assays for the ability of a microorganism to assimilate polysorbate 60 as a sole source of carbon and energy. Tween 60 polysorbate 60 polysorbate 60 assimilation hemicellulose assimilation assay Assays for the ability of a microorganism to assimilate hemicellulose as a sole source of carbon and energy. hemicellulose Carrine Blank hemicellulose assimilation guanine assimilation assay guanine Carrine Blank Assays for the ability of a microorganism to assimilate guanine as a sole source of carbon and energy. guanine assimilation guanidine assimilation assay guanidine Assays for the ability of a microorganism to assimilate guanidine as a sole source of carbon and energy. guanidine assimilation Carrine Blank glycylglycine assimilation assay glycylglycine Carrine Blank Gly-Gly glycylglycine assimilation Assays for the ability of a microorganism to assimilate glycylglycine as a sole source of carbon and energy. glycyl-glycyl-glycine assimilation assay Assays for the ability of a microorganism to assimilate glycyl-glycyl-glycine as a sole source of carbon and energy. Carrine Blank glycyl-glycyl-glycine assimilation Gly-Gly-Gly glycyl-glycyl-glycine glycolic acid assimilation assay glycolate assimilation Carrine Blank Assays for the ability of a microorganism to assimilate glycolate as a sole source of carbon and energy. glycolate glycolaldehyde assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate glycolaldehyde as a sole source of carbon and energy. glycolaldehyde assimilation glycolaldehyde glycine betaine assimilation assay glycine betaine assimilation glycine betaine Assays for the ability of a microorganism to assimilate glycine betaine as a sole source of carbon and energy. Carrine Blank glycine assimilation assay glycine glycine assimilation Assays for the ability of a microorganism to assimilate glycine as a sole source of carbon and energy. Carrine Blank L-glycine glycerol 1-phosphate assimilation assay glycerol 1-phosphate assimilation Carrine Blank glycerol 1-phosphate Assays for the ability of a microorganism to assimilate glycerol 1-phosphate as a sole source of carbon and energy. glyceric acid assimilation assay Assays for the ability of a microorganism to assimilate glycerate (glyceric acid) as a sole source of carbon and energy. DL-glycerate glyceric acid glycerate Carrine Blank glycerate assimilation glutathione assimilation assay Assays for the ability of a microorganism to assimilate glutathione as a sole source of carbon and energy. glutathione Carrine Blank glutathione assimilation glutaric acid assimilation assay glutarate glutaric acid Assays for the ability of a microorganism to assimilate glutarate (glutaric acid) as a sole source of carbon and energy. glutarate assimilation Carrine Blank glutamine assimilation assay glutamine assimilation glutamine Carrine Blank Assays for the ability of a microorganism to assimilate glutamine as a sole source of carbon and energy. gamma-poly(L-glutamic acid) assimilation assay gamma-poly(L-glutamic acid) assimilation gamma-poly(L-glutamic acid) Assays for the ability of a microorganism to assimilate gamma-poly(L-glutamic acid) as a sole source of carbon and energy. Carrine Blank glutamic acid assimilation assay Carrine Blank sodium glutamate glutamic acid glutamic acid assimilation glutatmate glutamate assimilation glutamate Assays for the ability of a microorganism to assimilate glutamic acid (glutamate) as a sole source of carbon and energy. glucuronic acid assimilation assay glucuronic acid assimilation glucuronate Carrine Blank GRTas glucuronate assimilation gluronate glucuronic acid Assays for the ability of a microorganism to assimilate glucuronate (glucuronic acid) as a sole source of carbon and energy. glucosiduronic acid assimilation assay beta-D-glucosiduronate glucosiduronic acid Assays for the ability of a microorganism to assimilate glucosiduronic acid (alpha-D-glucosiduronate or beta-D-glucosiduronate) as a sole source of carbon and energy. glucuronide Carrine Blank alpha-D-glucosiduronate glucosiduronic acid assimilation glucoside assimilation assay d-glucopyranoside Carrine Blank glucoside assimilation glucoside glucopyranoside d-glucoside Assays for the ability of a microorganism to assimilate glucoside as a sole source of carbon and energy. heptanoic acid fermentation assay heptanoate acidification heptanoic acid heptanoate heptanoate fermentation Assays for the ability of a microorganism to ferment heptanoate (heptanoic acid). Carrine Blank hexanoic acid fermentation assay Carrine Blank caproate capronate hexanoate hexanoic acid caproic acid hexanoate fermentation hexanoate acidification Assays for the ability of a microorganism to ferment hexanoate (hexanoic acid). hippurate fermentation assay Assays for the ability of a microorganism to ferment hippurate. hippurate fermentation hippurate acidification hippuric acid hippurate Carrine Blank sodium hippurate histidine fermentation assay Carrine Blank histidine histidine fermentation histidine acidification Assays for the ability of a microorganism to ferment histidine. L-histidine fermentation assay Assays for the ability of a microorganism to ferment L-histidine. Carrine Blank L-histidine fermentation L-histidine L-histidine acidification hyaluronic acid fermentation assay Assays for the ability of a microorganism to ferment hyaluronate (hyaluronic acid). hyaluronate fermentation hyaluronate hyaluronic acid hyaluronate acidification Carrine Blank hydantoin fermentation assay hydantoin acidification hydantoin fermentation Assays for the ability of a microorganism to ferment hydantoin. Carrine Blank hydantoin hydroxybenzoic acid fermentation assay Assays for the ability of a microorganism to ferment hydroxybenzoate (hydroxybenzoic acid). hydroxybenzoate fermentation hydroxybenzoate acidification Carrine Blank hydroxybenzoic acid hydroxybenzoate hydroxybutyric acid fermentation assay hydroxybutyric acid acidification hydroxybutyrate hydroxybutyric acid Assays for the ability of a microorganism to ferment hydroxybutyric acid (hydroxybutyrate). Carrine Blank hydroxybutyric acid fermentation hydroxyproline fermentation assay Carrine Blank hydroxyproline acidification Assays for the ability of a microorganism to ferment hydroxyproline. hydroxyproline fermentation hydroxyproline hydroxyquinoline fermentation assay hydroxyquinoline fermentation hydroxyquinoline hydroxyquinoline acidification Assays for the ability of a microorganism to ferment hydroxyquinoline. Carrine Blank hypoxanthine fermentation assay hypoxanthine acidification Carrine Blank Assays for the ability of a microorganism to ferment hypoxanthine. hypoxanthine hypoxanthine fermentation indole fermentation assay Assays for the ability of a microorganism to ferment indole. indol indlin indole indin indole acidification Carrine Blank indole fermentation indole-3-acetic acid fermentation assay indole-3-acetate fermentation Assays for the ability of a microorganism to ferment indole-3-acetate (indole-3-acetic acid). indole-3-acetic acid Carrine Blank indole acetic acid indoleacetic acid IAA indole-3-acetate acidification indole-3-acetate inosine fermentation assay inosine fermentation inosine Carrine Blank inosine acidification Assays for the ability of a microorganism to ferment inosine. isoamylol fermentation assay isoamylol fermentation isoamyl alcohol Assays for the ability of a microorganism to ferment isoamylol. Carrine Blank isoamylol acidification isoamylol isoamy alcohol isobutanol fermentation assay isobutanol fermentation iso-butanol isobutyl alcohol isobutanol acidification Carrine Blank Assays for the ability of a microorganism to ferment isobutanol. isobutanol 2-methyl-1-propanol isobutyric acid fermentation assay isobutyric acid Assays for the ability of a microorganism to ferment isobutyrate (isobutyric acid). isobutyrate iso-butyric acid Carrine Blank isobutyrate fermentation isobutyrate acidification iso-butanoic acid i-butyrate iso-butyrate isocaproic acid fermentation assay isocaproate isocaproate acidification Carrine Blank Assays for the ability of a microorganism to ferment isocaproate (isocaproic acid). isocaproate fermentation isocaproic acid isocitric acid fermentation assay isocitric acid fermentation isocitric acid Carrine Blank Assays for the ability of a microorganism to ferment isocitric acid (isocitrate). isocitric acid acidification isocitrate isoferulic acid fermentation assay 3-hydroxy-3-methoxycinnamate 3-hydroxy-3-methoxycinnamic acid isoferulic acid isoferulate Carrine Blank isoferulic acid fermentation Assays for the ability of a microorganism to ferment isoferulic acid. isoferulic acid acidification isoleucine fermentation assay isoleucine Assays for the ability of a microorganism to ferment isoleucine. Carrine Blank isoleucine fermentation isoleucine acidification L-isoleucine fermentation assay L-isoleucine L-isoleucine fermentation Assays for the ability of a microorganism to ferment L-isoleucine. L-isoleucine acidification Carrine Blank isovaleric acid fermentation assay Carrine Blank isovalerate fermentation isovaleric acid Assays for the ability of a microorganism to ferment isovalerate (isovaleric acid). isovalerate iso-valerate iso-valeric acid isovalerate acidification i-valerate itaconic acid fermentation assay itaconate Assays for the ability of a microorganism to ferment itaconic acid (itaconate). itaconic acid itaconic acid acidification itaconic acid fermentation Carrine Blank ketogluconic acid fermentation assay ketogluconate 2- or 5-ketogluconate Carrine Blank ketogluconate acidification Assays for the ability of a microorganism to ferment ketogluconate (ketogluconic acid). ketogluconate fermentation ketogluconic acid alpha-aminobutyric acid fermentation assay alpha-aminobutyrate acidification alpha-aminobutyrate alpha-aminobutyrate fermentation Carrine Blank alpha-aminobutyric acid Assays for the ability of a microorganism to ferment alpha-aminobutyrate (alpha-aminobutyric acid). l-alaninamide fermentation assay Carrine Blank alaninamide l-alaninamide acidification Assays for the ability of a microorganism to ferment l-alaninamide. l-alaninamide l-alaninamide fermentation lactamide fermentation assay lactamide fermentation Assays for the ability of a microorganism to ferment lactamide. lactamide acidification lactamide Carrine Blank lactic acid fermentation assay lactic acid DL-lactate D- and L-lactic acid DL-lactic acid Carrine Blank D,L-iactate Assays for the ability of a microorganism to ferment lactate (rac-lactic acid, or 2-hydroxypropanoic acid). DLlactate DL-iactate D,L-lacate lactate D- or L-lactate lactate acidification D,L-lactic acid both L- and D-lactate L- and D-lactic acid lactate fermentation (S)-lactic acid fermentation assay (S)-lactate fermentation Assays for the ability of a microorganism to ferment (S)-lactate. Carrine Blank (S)-lactate L-lactate isomer sodium L-lactate L-lactic acids Llactic acid (S)-lactate acidification lactose fermentation assay LAC lactose fermentation lactose lactose acidification Assays for the ability of a microorganism to ferment lactose. lacose Carrine Blank alpha-lactose fermentation assay D-lactose alpha-lactose acidification Dlactose Carrine Blank alpha-lactose fermentation alpha-lactose Assays for the ability of a microorganism to ferment alpha-lactose. alactose lactulose fermentation assay lactulose fermentation lactulose acidification Assays for the ability of a microorganism to ferment lactulose. lactulose Carrine Blank laminarin fermentation assay laminarin Assays for the ability of a microorganism to ferment laminarin. Carrine Blank laminarin acidification laminarin fermentation lecithin fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment lecithin. egg yolk lecithin lecithin fermentation lecithin lecithin acidification egg yolk factor Leu-Gly fermentation assay Leu-Gly fermentation leucyl glycine Leu-Gly Carrine Blank Assays for the ability of a microorganism to ferment Leu-Gly. Leu-Gly acidification leucine fermentation assay leucine fermentation Assays for the ability of a microorganism to ferment leucine. xxx leucine acidification Carrine Blank L-leucine fermentation assay L-leucine fermentation L-leucine Carrine Blank Assays for the ability of a microorganism to ferment L-leucine. L-leucine acidification lichenin fermentation assay lichenan lichenin Assays for the ability of a microorganism to ferment lichenin. lichenin fermentation lichenin acidification Carrine Blank lignin fermentation assay Assays for the ability of a microorganism to ferment lignin. lignin fermentation Carrine Blank lignin acidification lignin lysine fermentation assay lysine fermentation Carrine Blank lysine lysine acidification Assays for the ability of a microorganism to ferment lysine. DL-lysine L-lysine fermentation assay L-lysine acidification L-lysine chloride Carrine Blank L-lysine L-lysine fermentation Assays for the ability of a microorganism to ferment L-lysine. lyxose fermentation assay LYX Carrine Blank lyxose acidification lyxose fermentation lyxose Assays for the ability of a microorganism to ferment lyxose. methoxybenzoic acid assimilation assay anisic acid methoxybenzoate Carrine Blank Assays for the ability of a microorganism to assimilate methoxybenzoate (methoxybenzoic acid) as a sole source of carbon and energy. methoxybenzoic acid methoxybenzoate assimilation methoxybenzene assimilation assay Assays for the ability of a microorganism to assimilate methoxybenzene as a sole source of carbon and energy. Carrine Blank anisole methoxybenzene methoxybenzenoids methoxybenzene assimilation methionine assimilation assay methionine assimilation methionine DL-methioinine DL-ethionine Carrine Blank Assays for the ability of a microorganism to assimilate methionine as a sole source of carbon and energy. methanol assimilation assay methanol methanol assimilation Assays for the ability of a microorganism to assimilate methanol as a sole source of carbon and energy. Carrine Blank L-lysine assimilation assay L-lysine assimilation L-lysine L-lysine chloride Carrine Blank Assays for the ability of a microorganism to assimilate L-lysine as a sole source of carbon and energy. lysine assimilation assay lysine assimilation Carrine Blank lysine DL-lysine Assays for the ability of a microorganism to assimilate lysine as a sole source of carbon and energy. lignin assimilation assay Carrine Blank lignin lignin assimilation Assays for the ability of a microorganism to assimilate lignin as a sole source of carbon and energy. lichenin assimilation assay Assays for the ability of a microorganism to assimilate lichenin as a sole source of carbon and energy. lichenan Carrine Blank lichenin assimilation lichenin leucine assimilation assay Assays for the ability of a microorganism to assimilate leucine as a sole source of carbon and energy. leucine assimilation leucine Carrine Blank Leu-Gly assimilation assay leucyl glycine Leu-Gly assimilation Carrine Blank Leu-Gly Assays for the ability of a microorganism to assimilate Leu-Gly as a sole source of carbon and energy. lecithin assimilation assay lecithin Assays for the ability of a microorganism to assimilate lecithin as a sole source of carbon and energy. lecithin assimilation Carrine Blank egg yolk factor egg yolk lecithin laminarin assimilation assay laminarin Carrine Blank Assays for the ability of a microorganism to assimilate laminarin as a sole source of carbon and energy. laminarin assimilation lactose assimilation assay lacose Assays for the ability of a microorganism to assimilate lactose as a sole source of carbon and energy. lactose assimilation lactose Carrine Blank LACa ketogluconic acid assimilation assay ketogluconic acid Carrine Blank ketogluconate assimilation ketogluconate Assays for the ability of a microorganism to assimilate ketogluconate (ketogluconic acid) as a sole source of carbon and energy. 2- or 5-ketogluconate isovaleric acid assimilation assay isovalerate assimilation iso-valeric acid iso-valerate i-valerate isovaleric acid Carrine Blank Assays for the ability of a microorganism to assimilate isovalerate (isovaleric acid) as a sole source of carbon and energy. isovalerate L-isoleucine assimilation assay L-isoleucine assimilation Carrine Blank Assays for the ability of a microorganism to assimilate L-isoleucine as a sole source of carbon and energy. L-isoleucine isoleucine assimilation assay isoleucine isoleucine assimilation Assays for the ability of a microorganism to assimilate isoleucine as a sole source of carbon and energy. Carrine Blank isoferulic acid assimilation assay isoferulic acid Carrine Blank 3-hydroxy-3-methoxycinnamate isoferulic acid assimilation 3-hydroxy-3-methoxycinnamic acid isoferulate Assays for the ability of a microorganism to assimilate isoferulic acid as a sole source of carbon and energy. isocitric acid assimilation assay isocitric acid assimilation isocitric acid Assays for the ability of a microorganism to assimilate isocitric acid (isocitrate) as a sole source of carbon and energy. isocitrate Carrine Blank isocaproic acid assimilation assay Assays for the ability of a microorganism to assimilate isocaproate (isocaproic acid) as a sole source of carbon and energy. isocaproate assimilation isocaproic acid Carrine Blank isocaproate isobutyric acid assimilation assay iso-butyric acid isobutyrate assimilation Carrine Blank iso-butanoic acid Assays for the ability of a microorganism to assimilate isobutyrate (isobutyric acid) as a sole source of carbon and energy. isobutyric acid iso-butyrate isobutyrate i-butyrate isobutanol assimilation assay iso-butanol isobutyl alcohol isobutanol Assays for the ability of a microorganism to assimilate isobutanol as a sole source of carbon and energy. Carrine Blank isobutanol assimilation 2-methyl-1-propanol isoamylol assimilation assay Carrine Blank isoamy alcohol isoamylol isoamylol assimilation isoamyl alcohol Assays for the ability of a microorganism to assimilate isoamylol as a sole source of carbon and energy. indole-3-acetic acid assimilation assay indole-3-acetate IAA Carrine Blank indole-3-acetic acid Assays for the ability of a microorganism to assimilate indole-3-acetate (indole-3-acetic acid) as a sole source of carbon and energy. indole acetate indole-acetic acid indole-3-acetate assimilation indole assimilation assay Carrine Blank indole assimilation indin indlin indole Assays for the ability of a microorganism to assimilate indole as a sole source of carbon and energy. indol hypoxanthine assimilation assay hypoxanthine hypoxanthine assimilation Assays for the ability of a microorganism to assimilate hypoxanthine as a sole source of carbon and energy. Carrine Blank hydroxyquinoline assimilation assay Carrine Blank hydroxyquinoline Assays for the ability of a microorganism to assimilate hydroxyquinoline as a sole source of carbon and energy. hydroxyquinoline assimilation hydroxybutyric acid assimilation assay hydroxybutyric acid hydroxybutyrate hydroxybutyric acid assimilation Carrine Blank Assays for the ability of a microorganism to assimilate hydroxybutyric acid (hydroxybutyrate) as a sole source of carbon and energy. hydroxybenzoic acid assimilation assay hydroxybenzoate assimilation Carrine Blank hydroxybenzoic acid hydroxybenzoate Assays for the ability of a microorganism to assimilate hydroxybenzoate (hydroxybenzoic acid) as a sole source of carbon and energy. hydantoin assimilation assay hydantoin assimilation Assays for the ability of a microorganism to assimilate hydantoin as a sole source of carbon and energy. Carrine Blank hydantoin hyaluronic acid assimilation assay Assays for the ability of a microorganism to assimilate hyaluronate (hyaluronic acid) as a sole source of carbon and energy. hyaluronic acid Carrine Blank hyaluronate hyaluronate assimilation histidine assimilation assay histidine assimilation Carrine Blank histidine HIS Assays for the ability of a microorganism to assimilate histidine as a sole source of carbon and energy. hippurate assimilation assay hippurate assimilation sodium hippurate hippurate Carrine Blank hippuric acid Assays for the ability of a microorganism to assimilate hippurate as a sole source of carbon and energy. hexanoic acid assimilation assay hexanoate hexanoic acid Carrine Blank capronate caproate caproic acid hexanoate assimilation Assays for the ability of a microorganism to assimilate hexanoate (hexanoic acid) as a sole source of carbon and energy. hexadecanoic acid assimilation assay palmitic acid hexadecanoate hexadecanoic acid palmitic Assays for the ability of a microorganism to assimilate hexadecanoate (hexadecanoic acid) as a sole source of carbon and energy. hexadecanoate assimilation Carrine Blank palmitate straight-chain saturated palmitic acid heptanoic acid assimilation assay heptanoate heptanoic acid heptanoate assimilation Carrine Blank Assays for the ability of a microorganism to assimilate heptanoate (heptanoic acid) as a sole source of carbon and energy. malic acid fermentation assay DL-sodium malate sodium DL-malate Assays for the ability of a microorganism to ferment malate (malic acid). malate fermentation malate DL-malic acid malate acidification DL-malate sodium malate Carrine Blank malic acid methyl D-galactoside fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment methyl D-galactoside. methyl D-galactoside fermentation methyl D-galactoside methyl D-galactoside acidification (S)-malic acid fermentation assay (S)-malic acid lMLTa (S)-malate(2-) acidification (S)-malate(2-) fermentation Assays for the ability of a microorganism to ferment (S)-malate(2-) or (S)-malic acid. Carrine Blank L-malate sodium L-malate L-malic acid (S)-malate(2-) maleic acid fermentation assay Assays for the ability of a microorganism to ferment maleate (maleic acid). maleate Carrine Blank maleate acidification maleic acid maleinate maleate fermentation maltitol fermentation assay maltitol acidification Assays for the ability of a microorganism to ferment maltitol. Carrine Blank maltitol D-maltitol maltitol fermentation maltodextrin fermentation assay maltodextrin Assays for the ability of a microorganism to ferment maltodextrin. maltodextrin fermentation maltodextrin acidification Carrine Blank maltose fermentation assay MAL maltose acidification maltose maltose fermentation Carrine Blank Assays for the ability of a microorganism to ferment maltose. malvalic acid fermentation assay Assays for the ability of a microorganism to ferment malvalic acid. malvalic acid fermentation malvalic acid malvalic acid acidification Carrine Blank malvalate mannan fermentation assay Assays for the ability of a microorganism to ferment mannan. mannan acidification mannan Carrine Blank mannan fermentation mannitol fermentation assay mannitoll mannite MAN mannitol acidification mannitol fermentation manitol mannitol Assays for the ability of a microorganism to ferment mannitol. Carrine Blank mannose fermentation assay mannose acidification mannose fermentation MNE mannose Carrine Blank Assays for the ability of a microorganism to ferment mannose. MANO mannoside fermentation assay D-mannoside mannoside acidification Assays for the ability of a microorganism to ferment mannoside. Carrine Blank mannoside mannoside fermentation Dmannopyranoside D-mannopyranoside mannopyranoside heptadecanoic acid fermentation assay margarate heptadecanoic acid Carrine Blank margarate fermentation Assays for the ability of a microorganism to ferment margarate (heptadecanoic acid). heptadecanoate margarate acidification melibiose fermentation assay Assays for the ability of a microorganism to ferment melibiose. melibose melibisse melibiose acidification melibiose Carrine Blank meliobiose melobiose melibiose fermentation mellibiose meliboise MEL mesaconic acid fermentation assay mesaconic acid acidification mesaconic acid fermentation mesaconate mesaconic acid Assays for the ability of a microorganism to ferment mesaconic acid (mesaconate). Carrine Blank meso-2,6-diaminopimelic acid fermentation assay meso-2,6-diaminopimelic acid meso-2,6-diaminopimelic acid acidification Carrine Blank meso-diaminopimelic acid meso-2,6-diaminopimelate meso-2,6-diaminopimelic acid fermentation Assays for the ability of a microorganism to ferment meso-2,6-diaminopimelic acid (meso-2,6-diaminopimelate). meso-diaminopimelate meso-tartaric acid fermentation assay meso-tartaric acid fermentation meso-tartaric acid Assays for the ability of a microorganism to ferment meso-tartaric acid (meso-tartrate). meso-tartaric acid acidification Carrine Blank meso-tartrate methanethiol fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment methanethiol. methanethiol methanethiol acidification methanethiol fermentation methanol fermentation assay methanol fermentation Carrine Blank methanol Assays for the ability of a microorganism to ferment methanol. methanol acidification methionine fermentation assay DL-ethionine methionine methionine fermentation Assays for the ability of a microorganism to ferment methionine. DL-methionine Carrine Blank methionine acidification L-methionine fermentation assay Assays for the ability of a microorganism to ferment L-methionine. Carrine Blank L-methionine acidification L-methionine L-methionine fermentation methoxybenzene fermentation assay Carrine Blank methoxybenzene acidification methoxybenzene anisole methoxybenzene fermentation methoxybenzenoids Assays for the ability of a microorganism to ferment methoxybenzene. methoxybenzoic acid fermentation assay anisic acid methoxybenzoic acid methoxybenzoate acidification methoxybenzoate fermentation Carrine Blank methoxybenzoate Assays for the ability of a microorganism to ferment methoxybenzoate (methoxybenzoic acid). rac-methyl lactate fermentation assay rac-methyl lactate acidification rac-methyl lactate fermentation Assays for the ability of a microorganism to ferment rac-methyl lactate. rac-methyl lactate methyl lactate lactic acid methyl ester Carrine Blank methyl (R)-lactate fermentation assay methyl (R)-lactate acidification Carrine Blank methyl (R)-lactate methyl (R)-lactate fermentation D-lactic acid methyl ester Assays for the ability of a microorganism to ferment methyl (R)-lactate. methyl alpha-D-galactoside fermentation assay methyl-a-D-galactoside methyl alpha-D-galactoside Assays for the ability of a microorganism to ferment methyl alpha-D-galactoside. methyl-a-galactopyranoside a-methyl-D-galactoside Carrine Blank methyl alpha-D-galactoside fermentation methyl alpha-D-galactoside acidification methyl beta-D-galactoside fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment methyl beta-D-galactoside. methyl beta-D-galactoside acidification b-methyl-D-galactoside methyl beta-D-galactoside methyl-b-D-galactoside methyl beta-galactopyranoside methyl beta-D-galactoside fermentation beta-methyl-D-galactoside methyl b-galactopyranoside N-acetylglucosamine fermentation assay Nacetyl-glucosamine N-acetylglucosamine NAG Carrine Blank N-acetylglucosamine acidification Nacetylglucosamine N-acetylglucosamine fermentation N-acetyl-glucosamine Assays for the ability of a microorganism to ferment N-acetylglucosamine. acetylglucosamine N-acetylglucosoamine N-acetylglycosamine NAG methyl pyruvate fermentation assay methylpyruvate Carrine Blank methyl pyruvate methyl pyruvate acidification pyruvic acid methyl ester Assays for the ability of a microorganism to ferment methyl pyruvate. pyruvatic acid methyl ester pyruvic acid methylester methyl pyruvate fermentation methyl pyruvic acid methylamine fermentation assay methylamine fermentation methylamine Assays for the ability of a microorganism to ferment methylamine. Carrine Blank monomethylamine methylamine acidification methyl glucoside fermentation assay methyl glucoside fermentation methyl glucoside Carrine Blank methyl glucoside acidification Assays for the ability of a microorganism to ferment methyl glucoside. methylmalonic acid fermentation assay methylmalonate methylmalonic acid methylmalonic acid fermentation Assays for the ability of a microorganism to ferment methylmalonic acid (methylmalonate). methylmalonic acid acidification Carrine Blank monomethyl succinate fermentation assay succinic acid mono-methyl ester succinic acid mono-methylester succinic acid mono methyl ester monomethyl succinate succinic acid mono-methyl-ester Assays for the ability of a microorganism to ferment monomethyl succinate. Carrine Blank monomethyl succinate fermentation succinic acid monomethyl ester monomethyl succinate acidification myo-inositol fermentation assay meso-inositol m-inositol myo-inositol fermentation myo-inositol myo-inositol acidification myoinositol i-inositol Carrine Blank mesoinositol Assays for the ability of a microorganism to ferment myo-inositol. inosite myo inositol N-acetyl-beta-D-galactosamine fermentation assay Carrine Blank N-acetyl-beta-D-galactosamine acidification Assays for the ability of a microorganism to ferment N-acetyl-beta-D-galactosamine. N-acetyl-beta-D-galactosamine fermentation N-acetyl-beta-D-galactosamine N-acetyl-beta-D-mannosamine fermentation assay Carrine Blank N-acetyl-beta-D-mannosamine acidification Assays for the ability of a microorganism to ferment N-acetyl-beta-D-mannosamine. N-acetyl-beta-D-mannosamine fermentation N-acetyl-beta-D-mannosamine N-acetyl-L-glutamic acid fermentation assay Carrine Blank N-acetyl-L-glutamic acid fermentation Assays for the ability of a microorganism to ferment N-acetyl-L-glutamic acid. N-acetyl-L-glutamate N-acetyl-L-glutamic acid acidification N-acetyl-L-glutamic acid N-acetylneuraminic acid fermentation assay N-acetylneuraminate N-acetylneuraminic acid fermentation N-acetylneuraminic acid N-acetyl-neuraminic acid N-acetylneuraminic acid acidification Assays for the ability of a microorganism to ferment N-acetylneuraminic acid (N-acetylneuraminate). Carrine Blank N-carbamoylglycine fermentation assay carbamoylglycine N-carbamoylglycine acidification Carrine Blank N-carbamoylglycine fermentation N-carbamoylglycine Assays for the ability of a microorganism to ferment N-carbamoylglycine. N-carbamoylsarcosine fermentation assay Assays for the ability of a microorganism to ferment N-carbamoylsarcosine. N-carbamoylsarcosine fermentation N-carbamoylsarcosine N-carbamoylsarcosine acidification carbamoylsarcosine Carrine Blank N-methyl-D-aspartic acid fermentation assay Assays for the ability of a microorganism to ferment N-methyl-D-aspartic acid. b-methylaspartate Carrine Blank beta-methylaspartate b-methylaspartic acid N-methyl-D-aspartic acid fermentation beta-methylaspartic acid N-methyl-D-aspartic acid acidification N-methyl-D-aspartic acid N-methyl-L-alanine fermentation assay N-methyl-L-alanine N-methyl-L-alanine acidification alanine methyl ester Assays for the ability of a microorganism to ferment N-methyl-L-alanine. N-methyl-L-alanine fermentation Carrine Blank N-methylhydantoin fermentation assay Assays for the ability of a microorganism to ferment N-methylhydantoin. N-methylhydantoin acidification N-methylhydantoin N-methylhydantoin fermentation Carrine Blank N-methylnicotinic acid fermentation assay N-methylnicotinate fermentation N-methylnicotinate N-methylnicotinic acid Carrine Blank Assays for the ability of a microorganism to ferment N-methylnicotinate (N-methylnicotinic acid). trigonelline N-methylnicotinate acidification N,N-dimethylethanolamine fermentation assay N,N-dimethylethanolamine acidification N,N-dimethylethanolamine N,N-dimethylethanolamine fermentation dimethylethanolamine Carrine Blank Assays for the ability of a microorganism to ferment N,N-dimethylethanolamine. N,N-dimethylglycine fermentation assay N,N-dimethylglycine Carrine Blank N,N-dimethylglycine acidification Assays for the ability of a microorganism to ferment N,N-dimethylglycine. N,N-dimethylglycine fermentation dimethylglycine nonanoic acid fermentation assay nonanoate acidification nonanoate fermentation Assays for the ability of a microorganism to ferment nonanoate (nonanoic acid). nonanoic acid nonanoate Carrine Blank o-cresol fermentation assay o-cresol o-cresol acidification Assays for the ability of a microorganism to ferment o-cresol. Carrine Blank o-cresol fermentation methanethiol assimilation assay methanethiol assimilation methanethiol Assays for the ability of a microorganism to assimilate methanethiol as a sole source of carbon and energy. Carrine Blank meso-2,6-diaminopimelic acid assimilation assay Carrine Blank meso-2,6-diaminopimelic acid meso-diaminopimelate meso-2,6-diaminopimelic acid assimilation meso-diaminopimelic acid Assays for the ability of a microorganism to assimilate meso-2,6-diaminopimelic acid (meso-2,6-diaminopimelate) as a sole source of carbon and energy. meso-2,6-diaminopimelate mesaconic acid assimilation assay mesaconic acid mesaconic acid assimilation Carrine Blank mesaconate Assays for the ability of a microorganism to assimilate mesaconic acid (mesaconate) as a sole source of carbon and energy. melibiose assimilation assay Carrine Blank MEL meliboise melibiose assimilation melibisse melobiose melibiose meliobiose melibose Assays for the ability of a microorganism to assimilate melibiose as a sole source of carbon and energy. mellibiose heptadecanoic acid assimilation assay Carrine Blank heptadecanoate margarate margarate assimilation heptadecanoic acid Assays for the ability of a microorganism to assimilate margarate (heptadecanoic acid) as a sole source of carbon and energy. mannoside assimilation assay Carrine Blank mannoside assimilation Dmannopyranoside mannopyranoside D-mannopyranoside mannoside Assays for the ability of a microorganism to assimilate mannoside as a sole source of carbon and energy. D-mannoside mannose assimilation assay Assays for the ability of a microorganism to assimilate mannose as a sole source of carbon and energy. mannose mannose assimilation Carrine Blank MNE mannitol assimilation assay mannitol assimilation mannite MAN mannitoll Assays for the ability of a microorganism to assimilate mannitol as a sole source of carbon and energy. Carrine Blank manitol mannitol malvalic acid assimilation assay malvalate Carrine Blank malvalic acid Assays for the ability of a microorganism to assimilate malvalic acid as a sole source of carbon and energy. malvalic acid assimilation maltose assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate maltose as a sole source of carbon and energy. maltose maltose assimilation MAL maltodextrin assimilation assay maltodextrin Assays for the ability of a microorganism to assimilate maltodextrin as a sole source of carbon and energy. Carrine Blank maltodextrin assimilation maltitol assimilation assay Assays for the ability of a microorganism to assimilate maltitol as a sole source of carbon and energy. D-maltitol maltitol Carrine Blank maltitol assimilation maleic acid assimilation assay Assays for the ability of a microorganism to assimilate maleate (maleic acid) as a sole source of carbon and energy. maleic acid Carrine Blank maleate assimilation maleate maleinate malic acid assimilation assay Assays for the ability of a microorganism to assimilate malate (malic acid) as a sole source of carbon and energy. malic acid assimilation MLT malate assimilation sodium malate DL-sodium malate DL-malate DL-malic acid malic acid malate sodium DL-malate Carrine Blank MLT D-lyxose assimilation assay D-iyxose Carrine Blank Assays for the ability of a microorganism to assimilate D-lyxose as a sole source of carbon and energy. Dlyxose D-lyxose assimilation D-lyxose lyxose assimilation assay lyxose assimilation Assays for the ability of a microorganism to assimilate lyxose as a sole source of carbon and energy. Carrine Blank lyxose polysorbate 20 assimilation assay Tween 20 polysorbate 20 Tween 20 assimilation Carrine Blank Assays for the ability of a microorganism to assimilate polysorbate 20 as a sole source of carbon and energy. polysorbate 20 assimilation polysorbate assimilation assay Carrine Blank polysorbate assimilation Assays for the ability of a microorganism to assimilate polysorbate as a sole source of carbon and energy. Tweens polysorbate poly(hydroxybutyrate) assimilation assay poly-beta-hydroxybutyrate polyhydroxybutyrate Carrine Blank PHB poly-b-hydroxybutyric acid Assays for the ability of a microorganism to assimilate poly(hydroxybutyrate) or poly-beta-hydroxybutyrate as a sole source of carbon and energy. poly(hydroxybutyrate) assimilation poly(hydroxybutyrate) poly-b-hydroxybutyrate polygalacturonates assimilation assay polygalacturonates assimilation Carrine Blank Assays for the ability of a microorganism to assimilate polygalacturonates as a sole source of carbon and energy. polygalacturonic acids polygalacturonates methyl glucoside assimilation assay methyl glucoside assimilation Carrine Blank methyl glucoside Assays for the ability of a microorganism to assimilate methyl glucoside as a sole source of carbon and energy. picolinic acid assimilation assay picolinate Assays for the ability of a microorganism to assimilate picolinate (picolinic acid) as a sole source of carbon and energy. Carrine Blank picolinic acid picolinate assimilation phthalic acid assimilation assay phthalic acid phthalic acid assimilation potassium hydrogen phthalate Assays for the ability of a microorganism to assimilate phthalic acid (phthalate) as a sole source of carbon and energy. phthalate Carrine Blank phosphocholine assimilation assay phosphocholine phosphorylcholine Assays for the ability of a microorganism to assimilate phosphocholine as a sole source of carbon and energy. phosphocholine assimilation Carrine Blank phosphatidylcholine assimilation assay Assays for the ability of a microorganism to assimilate phosphatidylcholine as a sole source of carbon and energy. Carrine Blank phosphatidylcholine assimilation phosphatidylcholine phloroglucinol assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate phloroglucinol as a sole source of carbon and energy. phloroglucinol phloroglucinol assimilation phenylalanine assimilation assay Assays for the ability of a microorganism to assimilate phenylalanine as a sole source of carbon and energy. Carrine Blank phenylalanine assimilation phenylalanine phenol assimilation assay Carrine Blank phenol Assays for the ability of a microorganism to assimilate phenol as a sole source of carbon and energy. phenol assimilation perseitol assimilation assay Assays for the ability of a microorganism to assimilate perseitol as a sole source of carbon and energy. Carrine Blank perseitol assimilation perseitol pentenoic acid assimilation assay Assays for the ability of a microorganism to assimilate pentenoate (pentanoic acid) as a sole source of carbon and energy. pentenoate Carrine Blank pentenoate assimilation pentanoic acid pentan-1-ol assimilation assay pentan-1-ol pentanol pentan-1-ol assimilation 1-pentanol Assays for the ability of a microorganism to assimilate pentan-1-ol as a sole source of carbon and energy. Carrine Blank pentadecanoic acid assimilation assay pentadecanoic acid pentadecanoate assimilation Carrine Blank Assays for the ability of a microorganism to assimilate pentadecanoate (pentadecanoic acid) as a sole source of carbon and energy. pentadecanoate p-cresol assimilation assay Assays for the ability of a microorganism to assimilate p-cresol as a sole source of carbon and energy. p-cresol Carrine Blank p-cresol assimilation oxopentanoic acid assimilation assay oxopentanoic acid Carrine Blank oxopentanoates assimilation oxopentanoates ketovalerate ketovaleric acid Assays for the ability of a microorganism to assimilate oxopentanoates (oxopentanoic acid) as a sole source of carbon and energy. oxaloacetic acid assimilation assay Carrine Blank oxaloacetic acid OAA oxaloacetate oxaloacetic acid assimilation Assays for the ability of a microorganism to assimilate oxaloacetic acid (oxaloacetate) as a sole source of carbon and energy. oxalic acid assimilation assay oxalate ammonium oxalate Carrine Blank oxalate assimilation oxalic acid Assays for the ability of a microorganism to assimilate oxalate (oxalic acid) as a sole source of carbon and energy. ornithine assimilation assay ornithine assimilation Assays for the ability of a microorganism to assimilate ornithine as a sole source of carbon and energy. ornithine Carrine Blank oleic acid assimilation assay oleate Carrine Blank oleic acid assimilation Assays for the ability of a microorganism to assimilate oleic acid (oleate) as a sole source of carbon and energy. oleic acid octanol assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate octanol as a sole source of carbon and energy. octanol assimilation octanol octanoic acid assimilation assay octanoic acid caprylate Carrine Blank Assays for the ability of a microorganism to assimilate octanoate (octanoic acid) as a sole source of carbon and energy. octanoate assimilation octanoate caprylic acid o-cresol assimilation assay Carrine Blank o-cresol assimilation Assays for the ability of a microorganism to assimilate o-cresol as a sole source of carbon and energy. o-cresol nonanoic acid assimilation assay nonanoate nonanoate assimilation Assays for the ability of a microorganism to assimilate nonanoate (nonanoic acid) as a sole source of carbon and energy. nonanoic acid Carrine Blank N,N-dimethylglycine assimilation assay Carrine Blank dimethylglycine N,N-dimethylglycine N,N-dimethylglycine assimilation Assays for the ability of a microorganism to assimilate N,N-dimethylglycine as a sole source of carbon and energy. N,N-dimethylethanolamine assimilation assay N,N-dimethylethanolamine assimilation Carrine Blank dimethylethanolamine Assays for the ability of a microorganism to assimilate N,N-dimethylethanolamine as a sole source of carbon and energy. N,N-dimethylethanolamine N-methylnicotinic acid assimilation assay trigonelline Assays for the ability of a microorganism to assimilate N-methylnicotinate (N-methylnicotinic acid) as a sole source of carbon and energy. N-methylnicotinate Carrine Blank N-methylnicotinic acid N-methylnicotinate assimilation N-methylhydantoin assimilation assay Assays for the ability of a microorganism to assimilate N-methylhydantoin as a sole source of carbon and energy. N-methylhydantoin assimilation N-methylhydantoin Carrine Blank N-methyl-L-alanine assimilation assay Carrine Blank alanine methyl ester N-methyl-L-alanine assimilation N-methyl-L-alanine Assays for the ability of a microorganism to assimilate N-methyl-L-alanine as a sole source of carbon and energy. N-methyl-D-aspartic acid assimilation assay Assays for the ability of a microorganism to assimilate N-methyl-D-aspartic acid as a sole source of carbon and energy. N-methyl-D-aspartic acid assimilation beta-methylaspartic acid b-methylaspartate beta-methylaspartate N-methyl-D-aspartic acid b-methylaspartic acid Carrine Blank N-carbamoylsarcosine assimilation assay N-carbamoylsarcosine Assays for the ability of a microorganism to assimilate N-carbamoylsarcosine as a sole source of carbon and energy. carbamoylsarcosine Carrine Blank N-carbamoylsarcosine assimilation N-carbamoylglycine assimilation assay Assays for the ability of a microorganism to assimilate N-carbamoylglycine as a sole source of carbon and energy. carbamoylglycine N-carbamoylglycine assimilation N-carbamoylglycine Carrine Blank N-acetyl-beta-D-mannosamine assimilation assay N-acetyl-beta-mannosamine N-acetyl-beta-D-mannosamine assimilation N-acetyl-beta-D-mannosamine Assays for the ability of a microorganism to assimilate N-acetyl-beta-D-mannosamine as a sole source of carbon and energy. N-acetyl-b-mannosmaine N-acetyl-beta-mannosamine assimilation Carrine Blank N-acetyl-b-mannosmaine assimilation methylmalonic acid assimilation assay Assays for the ability of a microorganism to assimilate methylmalonic acid (methylmalonate) as a sole source of carbon and energy. Carrine Blank methylmalonate methylmalonic acid assimilation methylmalonic acid beta-lactose assimilation assay Assays for the ability of a microorganism to assimilate beta-lactose as a sole source of carbon and energy. Carrine Blank beta-lactose beta-lactose assimilation octanoic acid fermentation assay Assays for the ability of a microorganism to ferment octanoate (octanoic acid). caprylic acid octanoic acid octanoate caprylate octanoate fermentation Carrine Blank octanoate acidification octanol fermentation assay octanol octanol fermentation Carrine Blank octanol acidification Assays for the ability of a microorganism to ferment octanol. oleic acid fermentation assay oleate Assays for the ability of a microorganism to ferment oleic acid (oleate). oleic acid acidification oleic acid fermentation oleic acid Carrine Blank ornithine fermentation assay ornithine acidification Assays for the ability of a microorganism to ferment ornithine. Carrine Blank ornithine ornithine fermentation L-ornithine fermentation assay L-ornithine Assays for the ability of a microorganism to ferment L-ornithine. L-ornithine acidification Carrine Blank L-ornithine fermentation oxalic acid fermentation assay oxalate acidification Carrine Blank oxalic acid ammonium oxalate Assays for the ability of a microorganism to ferment oxalate (oxalic acid). oxalate oxalate fermentation oxaloacetic acid fermentation assay oxaloacetic acid fermentation oxaloacetic acid Carrine Blank oxaloacetic acid acidification Assays for the ability of a microorganism to ferment oxaloacetic acid (oxaloacetate). oxaloacetate OAA oxopentanoic acid fermentation assay oxopentanoic acid oxopentanoates fermentation oxopentanoates Assays for the ability of a microorganism to ferment oxopentanoates. Carrine Blank oxopentanoates acidification p-cresol fermentation assay Carrine Blank p-cresol fermentation p-cresol p-cresol acidification Assays for the ability of a microorganism to ferment p-cresol. pectin fermentation assay pectin acidification pectin pectin fermentation Assays for the ability of a microorganism to ferment pectin. Carrine Blank pecin pentadecanoic acid fermentation assay pentadecanoate fermentation Assays for the ability of a microorganism to ferment pentadecanoate (pentadecanoic acid). Carrine Blank pentadecanoic acid pentadecanoate acidification pentadecanoate pentan-1-ol fermentation assay Assays for the ability of a microorganism to ferment pentan-1-ol. pentan-1-ol acidification pentan-1-ol fermentation 1-pentanol pentan-1-ol Carrine Blank pentanol pentenoic acid fermentation assay pentenoate Assays for the ability of a microorganism to ferment pentenoate (pentanoic acid). pentanoic acid Carrine Blank pentenoate acidification pentenoate fermentation perseitol fermentation assay Assays for the ability of a microorganism to ferment perseitol. perseitol acidification perseitol fermentation Carrine Blank perseitol phenol fermentation assay Assays for the ability of a microorganism to ferment phenol. Carrine Blank phenol phenol fermentation phenol acidification trehalose fermentation assay tregalose TRE trehalose trehalose fermentation treharose threhalose Assays for the ability of a microorganism to ferment trehalose. trehalose acidification Carrine Blank salicyl alcohol fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment salicyl alcohol. salicyl alcohol acidification salicyl alcohol salicyl alcohol fermentation saligenin rutin fermentation assay Carrine Blank rutin Assays for the ability of a microorganism to ferment rutin. rutin fermentation rutin acidification ribose fermentation assay ribose RIB Carrine Blank Assays for the ability of a microorganism to ferment ribose. ribose fermentation ribose acidification ribitol fermentation assay ribitol fermentation adoniol adonitol acidification ADOn Assays for the ability of a microorganism to ferment ribitol. Carrine Blank ADO ribitol acidification adonitol fermentation adonitol adonite ribitol rhamnose fermentation assay Assays for the ability of a microorganism to ferment rhamnose. rhaninose rhamnose acidification rhammose ramnose rnannose rhimnose hamnose Carrine Blank rhamnose fermentation RHA rhamnose rham-nose quinic acid fermentation assay Assays for the ability of a microorganism to ferment quinate (quinic acid). quinate acidification quinate quinic acid quinate fermentation Carrine Blank L-pyrrolysine fermentation assay L-pyrrolysine fermentation L-pyrrolysine acidification L-pyrrolysine Carrine Blank Assays for the ability of a microorganism to ferment L-pyrrolysine. pyrrolysine fermentation assay Assays for the ability of a microorganism to ferment pyrrolysine. pyrrolysine acidification pyrrolysine Carrine Blank pyrrolysine fermentation pyrogallol fermentation assay pyrogallol fermentation Assays for the ability of a microorganism to ferment pyrogallol. pyrogallol pyrogallol acidification Carrine Blank pyrazinecarboxamide fermentation assay pyrazinecarboxamide fermentation pyrazinamide pyrazinecarboxamide Carrine Blank pyrazinecarboxamide acidification Assays for the ability of a microorganism to ferment pyrazinecarboxamide. putrescine fermentation assay putrescine Carrine Blank putrescine acidification putrescine fermentation Assays for the ability of a microorganism to ferment putrescine. purines fermentation assay purines acidification Assays for the ability of a microorganism to ferment purines. purines fermentation purines Carrine Blank D-psicose fermentation assay D-psicose D-psicose fermentation D-psicose acidification Assays for the ability of a microorganism to ferment D-psicose. Carrine Blank psicose fermentation assay Carrine Blank psicose psicose fermentation psicose acidification Assays for the ability of a microorganism to ferment psicose. propionic acid fermentation assay sodium propionate propionic acid propionate fermentation Carrine Blank propionic Assays for the ability of a microorganism to ferment propionate (propionic acid). propionate propionate acidification propanediol fermentation assay propanediol acidification Assays for the ability of a microorganism to ferment propanediol. propanediol Carrine Blank propanediol fermentation propane-1,3-diol fermentation assay 1,3-propanediol propane-1,3-diol propane-1,3-diol fermentation Carrine Blank Assays for the ability of a microorganism to ferment propane-1,3-diol. propane-1,3-diol acidification propane-1,2-diol fermentation assay Carrine Blank propane-1,2-diol propane-1,2-diol acidification propane-1,2-diol fermentation 1,2-propandiol propyleneglycol Assays for the ability of a microorganism to ferment propane-1,2-diol. 1,2-propanediol propylene glycol propan-2-ol fermentation assay 2-propanol propan-2-ol fermentation isopropanol Carrine Blank propan-2-ol acidification propan-2-ol iso-propanol Assays for the ability of a microorganism to ferment propan-2-ol. i-propanol propan-1-ol fermentation assay Carrine Blank 1-propanol n-propanol propanol Assays for the ability of a microorganism to ferment propan-1-ol. propan-1-ol acidification propan-1-ol propan-1-ol fermentation L-proline fermentation assay Carrine Blank L-proline fermentation Assays for the ability of a microorganism to ferment L-proline. L-proline L-proline acidification proline fermentation assay proline proline acidification proline fermentation Assays for the ability of a microorganism to ferment proline. Carrine Blank polysorbate 80 fermentation assay Carrine Blank polysorbate 80 polysorbate 80 fermentation polysorbate 80 acidification Tween 80 Assays for the ability of a microorganism to ferment polysorbate 80. polysorbate 60 fermentation assay polysorbate 60 fermentation Carrine Blank polysorbate 60 acidification Assays for the ability of a microorganism to ferment polysorbate 60. polysorbate 60 Tween 60 polysorbate 40 fermentation assay Carrine Blank Tween 40 Assays for the ability of a microorganism to ferment polysorbate 40. polysorbate 40 polysorbate 40 fermentation polysorbate 40 acidification polysorbate 20 fermentation assay polysorbate 20 fermentation polysorbate 20 Assays for the ability of a microorganism to ferment polysorbate 20. Carrine Blank polysorbate 20 acidification Tween 20 polysorbate fermentation assay Assays for the ability of a microorganism to ferment polysorbate. Carrine Blank polysorbate polysorbate acidification polysorbate fermentation Tweens poly(hydroxybutyrate) fermentation assay PHB Assays for the ability of a microorganism to ferment poly(hydroxybutyrate) or poly-beta-hydroxybutyrate. poly-beta-hydroxybutyrate poly(hydroxybutyrate) acidification poly(hydroxybutyrate) poly(hydroxybutyrate) fermentation poly-b-hydroxybutyric acid poly-b-hydroxybutyrate Carrine Blank polygalacturonates fermentation assay Assays for the ability of a microorganism to ferment polygalacturonates. polygalacturonates acidification polygalacturonic acids polygalacturonates polygalacturonates fermentation Carrine Blank poly(ethylene glycol) fermentation assay polyethylene glycol Assays for the ability of a microorganism to ferment poly(ethylene glycol). poly(ethylene glycol) poly(ethylene glycol) fermentation poly(ethylene glycol) acidification PEG Carrine Blank picolinic acid fermentation assay Carrine Blank picolinic acid picolinate acidification picolinate Assays for the ability of a microorganism to ferment picolinate (picolinic acid). picolinate fermentation phthalic acid fermentation assay phthalic acid acidification phthalic acid phthalic acid fermentation Carrine Blank potassium hydrogen phthalate phthalate Assays for the ability of a microorganism to ferment phthalic acid (phthalate). phosphocholine fermentation assay Carrine Blank phosphocholine acidification Assays for the ability of a microorganism to ferment phosphocholine. phosphorylcholine phosphocholine fermentation phosphocholine phosphatidylcholine fermentation assay phosphatidylcholine fermentation Carrine Blank Assays for the ability of a microorganism to ferment phosphatidylcholine. phosphatidylcholine phosphatidylcholine acidification phloroglucinol fermentation assay phloroglucinol acidification Assays for the ability of a microorganism to ferment phloroglucinol. phloroglucinol Carrine Blank phloroglucinol fermentation L-phenylalanine fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment L-phenylalanine. L-phenylalanine acidification L-phenylalanine L-phenylalanine fermentation phenylalanine fermentation assay Carrine Blank phenylalanine acidification phenylalanine fermentation phenylalanine Assays for the ability of a microorganism to ferment phenylalanine. phenylacetic acid fermentation assay phenyl acetic acid phenylacetate fermentation phenylacetic acid o-toluic acid Carrine Blank phenyl acetate o-toluate phenylacetate phenylacetate acidification Assays for the ability of a microorganism to ferment phenylacetate (phenylacetic acid). propanediol assimilation assay propanediol assimilation Carrine Blank Assays for the ability of a microorganism to assimilate propanediol as a sole source of carbon and energy. propanediol psicose assimilation assay Assays for the ability of a microorganism to assimilate psicose as a sole source of carbon and energy. Carrine Blank psicose assimilation psicose pullulan assimilation assay pullan Carrine Blank pullulan assimilation Assays for the ability of a microorganism to assimilate pullulan as a sole source of carbon and energy. pullulan purines assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate purines as a sole source of carbon and energy. purines assimilation purines pyrazinecarboxamide assimilation assay pyrazinamide Assays for the ability of a microorganism to assimilate pyrazinecarboxamide as a sole source of carbon and energy. pyrazinecarboxamide pyrazinecarboxamide assimilation Carrine Blank pyrogallol assimilation assay Carrine Blank pyrogallol Assays for the ability of a microorganism to assimilate pyrogallol as a sole source of carbon and energy. pyrogallol assimilation pyrrolysine assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate pyrrolysine as a sole source of carbon and energy. pyrrolysine pyrrolysine assimilation L-pyrrolysine assimilation assay Carrine Blank L-pyrrolysine L-pyrrolysine assimilation Assays for the ability of a microorganism to assimilate L-pyrrolysine as a sole source of carbon and energy. rhamnose assimilation assay rnannose rhaninose rhamnose rhammose rhamnose assimilation hamnose rham-nose Assays for the ability of a microorganism to assimilate rhamnose as a sole source of carbon and energy. ramnose Carrine Blank RHA rhimnose D-ribitol assimilation assay D-ribitol assimilation D-adonitol assimilation Assays for the ability of a microorganism to assimilate D-ribitol as a sole source of carbon and energy. D-ribitol D-adonitol Carrine Blank D-adonito Dadonitol ribose assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate ribose as a sole source of carbon and energy. ribose assimilation RIB ribose rutin assimilation assay Assays for the ability of a microorganism to assimilate rutin as a sole source of carbon and energy. Carrine Blank rutin rutin assimilation salicyl alcohol assimilation assay salicyl alcohol assimilation Carrine Blank saligenin Assays for the ability of a microorganism to assimilate salicyl alcohol as a sole source of carbon and energy. salicyl alcohol sarcosine assimilation assay sarcosine assimilation Carrine Blank Assays for the ability of a microorganism to assimilate sarcosine as a sole source of carbon and energy. sarcosine sedoheptulose assimilation assay sedoheptulose Assays for the ability of a microorganism to assimilate sedoheptulose as a sole source of carbon and energy. sedoheptulose assimilation Carrine Blank selenocysteine assimilation assay selenocysteine Assays for the ability of a microorganism to assimilate selenocysteine as a sole source of carbon and energy. Carrine Blank selenocysteine assimilation serine assimilation assay Assays for the ability of a microorganism to assimilate serine as a sole source of carbon and energy. SER serin Carrine Blank serine assimilation serine sorbose assimilation assay DL-sorbose Assays for the ability of a microorganism to assimilate sorbose as a sole source of carbon and energy. sorbose assimilation sotbose sorbose Carrine Blank spermidine assimilation assay Carrine Blank spermidine spermidine assimilation Assays for the ability of a microorganism to assimilate spermidine as a sole source of carbon and energy. spermine assimilation assay Carrine Blank spermine spermine assimilation Assays for the ability of a microorganism to assimilate spermine as a sole source of carbon and energy. starch assimilation assay amidon sarch Assays for the ability of a microorganism to assimilate starch as a sole source of carbon and energy. starch Carrine Blank potato starch starch assimilation sterculic acid assimilation assay Carrine Blank sterculic acid assimilation sterculic acid sterculate Assays for the ability of a microorganism to assimilate sterculic acid as a sole source of carbon and energy. syringic acid assimilation assay Assays for the ability of a microorganism to assimilate syringate (syringic acid) as a sole source of carbon and energy. Carrine Blank syringate 4-hydroxy-3,5-dimethoxybenzoic acid syringate assimilation 4-hydroxy-3,5-dimethoxybenzoate syringic acid tagatose assimilation assay Assays for the ability of a microorganism to assimilate tagatose as a sole source of carbon and energy. tagatose Carrine Blank tagatose assimilation tartaric acid assimilation assay tartrate assimilation tartaric acid tartrate Carrine Blank DL-tartrate Assays for the ability of a microorganism to assimilate tartrate (tartaric acid) as a sole source of carbon and energy. DL-tartaric acid D- and L-tartrate tetradecanoic acid assimilation assay tetradecanoate Carrine Blank tetradecanoate assimilation tetradecanoic acid myristic acid Assays for the ability of a microorganism to assimilate tetradecanoate (tetradecanoic acid) as a sole source of carbon and energy. myristate thioacetamide assimilation assay Carrine Blank thioacetamide assimilation Assays for the ability of a microorganism to assimilate thioacetamide as a sole source of carbon and energy. thioacetamide thiocyanate assimilation assay Carrine Blank thiocyanate assimilation potassium thiocyanate thiocyanate Assays for the ability of a microorganism to assimilate thiocyanate as a sole source of carbon and energy. thioglycolic acid assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate thioglycolic acid (or thioglycolate) as a sole source of carbon and energy. thioglycolic acid assimilation sodium thioglycollate sodium thioglycolate thioglycollate thioglycolic acid thioglycolate threonine assimilation assay Assays for the ability of a microorganism to assimilate threonine as a sole source of carbon and energy. threonine threonine assimilation Carrine Blank threose assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate threose as a sole source of carbon and energy. threose assimilation threose thymine assimilation assay Assays for the ability of a microorganism to assimilate thymine as a sole source of carbon and energy. Carrine Blank thymine assimilation thymine toluene assimilation assay Carrine Blank toluene Assays for the ability of a microorganism to assimilate toluene as a sole source of carbon and energy. toluene assimilation trans-sinapic acid assimilation assay 5-dimethoxycinnamate trans-sinapate trans-sinapic acid sinapate sinapic acid trans-sinapate assimilation 5-dimethoxycinnamic acid Assays for the ability of a microorganism to assimilate trans-sinapate (trans-sinapic acid) as a sole source of carbon and energy. Carrine Blank xylene assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate xylene as a sole source of carbon and energy. xylene assimilation xylene xylan assimilation assay Carrine Blank xylan assimilation oat spelt xylan Assays for the ability of a microorganism to assimilate xylan as a sole source of carbon and energy. xylan xanthine assimilation assay Assays for the ability of a microorganism to assimilate xanthine as a sole source of carbon and energy. xanthine assimilation xanthine xanthin Carrine Blank veratraldehyde assimilation assay Carrine Blank 4-dimethoxybenzaldehyde veratraldehyde Assays for the ability of a microorganism to assimilate veratraldehyde as a sole source of carbon and energy. veratraldehyde assimilation trans-sinapic acid fermentation assay sinapate trans-sinapate fermentation trans-sinapic acid trans-sinapate 5-dimethoxycinnamate dimethoxycinnamate Assays for the ability of a microorganism to ferment trans-sinapate (trans-sinapic acid). sinapic acid trans-sinapate acidification 5-dimethoxycinnamic acid Carrine Blank trans-aconitic acid fermentation assay Assays for the ability of a microorganism to ferment trans-aconitic acid (trans-aconitate). trans-aconitate Carrine Blank transaconitate trans-aconitic acid acidification trans-aconitic acid fermentation trans-aconitic acid toluene fermentation assay toluene acidification Carrine Blank toluene toluene fermentation Assays for the ability of a microorganism to ferment toluene. thymine fermentation assay thymine acidification thymine fermentation thymine Carrine Blank Assays for the ability of a microorganism to ferment thymine. thymidine 5'-monophosphate fermentation assay Carrine Blank thymidine 5'-monophosphate acidification thymidine 5'-monophosphate fermentation TMP Assays for the ability of a microorganism to ferment thymidine 5'-monophosphate. thymidine 5'-monophosphate thymidine fermentation assay Carrine Blank thymidine thymidine fermentation thymidine acidification Assays for the ability of a microorganism to ferment thymidine. threose fermentation assay threose Carrine Blank threose acidification threose fermentation Assays for the ability of a microorganism to ferment threose. L-threonine fermentation assay L-threonine acidification Carrine Blank Assays for the ability of a microorganism to ferment L-threonine. L-threonine fermentation L-threonine threonine fermentation assay threonine threonine fermentation threonine acidification Assays for the ability of a microorganism to ferment threonine. Carrine Blank thioglycolic acid fermentation assay Assays for the ability of a microorganism to ferment thioglycolic acid (thioglycolate). thioglycolic acid acidification thioglycolic acid fermentation thioglycolic acid sodium thioglycolate thioglycolate Carrine Blank sodium thioglycollate thioglycollate thiocyanate fermentation assay Carrine Blank potassium thiocyanate thiocyanate acidification thiocyanate thiocyanate fermentation Assays for the ability of a microorganism to ferment thiocyanate. thioacetamide fermentation assay thioacetamide thioacetamide fermentation Carrine Blank thioacetamide acidification Assays for the ability of a microorganism to ferment thioacetamide. tetradecanoic acid fermentation assay tetradecanoic acid Assays for the ability of a microorganism to ferment tetradecanoate (tetradecanoic acid). tetradecanoate fermentation tetradecanoate tetradecanoate acidification myristate myristic acid Carrine Blank tartaric acid fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment tartrate (tartaric acid). D- and L-tartrate DL-tartaric acid tartrate fermentation tartaric acid tartrate acidification tartrate DL-tartrate tagatose fermentation assay tagatose TAG tagatose fermentation tagatose acidification Assays for the ability of a microorganism to ferment tagatose. Carrine Blank syringic acid fermentation assay 4-hydroxy-3,5-dimethoxybenzoic acid Carrine Blank Assays for the ability of a microorganism to ferment syringate (syringic acid). syringic acid 4-hydroxy-3,5-dimethoxybenzoate syringate dimethoxybenzoate syringate fermentation syringate acidification succinic acid fermentation assay Assays for the ability of a microorganism to ferment succinate (succinic acid). succinate succinic acid Carrine Blank succinic acids succinate acidification succinic succinate fermentation sodium succinate disodium succinate succinamic acid fermentation assay Carrine Blank succinamic acid fermentation succinamic acid Assays for the ability of a microorganism to ferment succinamic acid. succinamic acid acidification suberic acid fermentation assay Carrine Blank suberate Assays for the ability of a microorganism to ferment suberate (suberic acid). suberate acidification suberic acid suberate fermentation sterculic acid fermentation assay sterculic acid sterculate sterculic acid acidification sterculic acid fermentation Assays for the ability of a microorganism to ferment sterculic acid. Carrine Blank stachyose fermentation assay stachyose acidification stachyose fermentation stachyose Carrine Blank Assays for the ability of a microorganism to ferment stachyose. spermine fermentation assay spermine fermentation Carrine Blank spermine acidification spermine Assays for the ability of a microorganism to ferment spermine. spermidine fermentation assay spermidine spermidine acidification spermidine fermentation Carrine Blank Assays for the ability of a microorganism to ferment spermidine. sorbose fermentation assay sorbose acidification Carrine Blank SBE DL-sorbose sotbose sorbose fermentation Assays for the ability of a microorganism to ferment sorbose. sorbose D-serine fermentation assay D-serine fermentation Assays for the ability of a microorganism to ferment D-serine. D-serine acidification Carrine Blank D-serine L-serine fermentation assay L-serine Assays for the ability of a microorganism to ferment L-serine. L-serine fermentation L-serine acidification Lserine Carrine Blank serine fermentation assay serine serine acidification Assays for the ability of a microorganism to ferment serine. Carrine Blank serin serine fermentation selenocysteine fermentation assay selenocysteine acidification selenocysteine Assays for the ability of a microorganism to ferment selenocysteine. Carrine Blank selenocysteine fermentation sedoheptulose fermentation assay sedoheptulose Carrine Blank sedoheptulose acidification sedoheptulose fermentation Assays for the ability of a microorganism to ferment sedoheptulose. sedoheptulosan fermentation assay sedoheptulosan fermentation Assays for the ability of a microorganism to ferment sedoheptulosan. Carrine Blank sedoheptulosan sedoheptulosan acidification sebacic acid fermentation assay sebacate Carrine Blank Assays for the ability of a microorganism to ferment sebacate (sebacic acid). sebacic acid sebacate acidification sebacate fermentation sarcosine fermentation assay sarcosine fermentation sarcosine acidification Assays for the ability of a microorganism to ferment sarcosine. Carrine Blank sarcosine glycerol phosphate fermentation assay Carrine Blank DL-glycerol PO4 glycerol phosphate glycerol phosphate fermentation L-a-glycerol phosphate Assays for the ability of a microorganism to ferment glycerol phosphate. glycerol phosphate acidification DL-glycerol phosphate DL-a-glycerol phosphate tributyrin fermentation assay tributyrate LIP tributyrin tributyrin acidification Carrine Blank tributyrin fermentation glyceryl tributyrate EST Assays for the ability of a microorganism to ferment tributyrin. tricarballylic acid fermentation assay tricarballylate Assays for the ability of a microorganism to ferment tricarballylate (tricarballylic acid). tricarballylate acidification tricarballylic acid tricarballylate fermentation Carrine Blank trihydroxybenzoic acid fermentation assay trihydroxybenzoate trihydroxybenzoate acidification trihydroxybenzoate fermentation trihydroxybenzoic acid Carrine Blank Assays for the ability of a microorganism to ferment trihydroxybenzoate (trihydroxybenzoic acid). trimethylamine fermentation assay trimethylamine trimethylamine acidification Carrine Blank trimethylammonium chloride trimethylamine fermentation Assays for the ability of a microorganism to ferment trimethylamine. trimethlamine tryptamine fermentation assay Carrine Blank tryptamine tryptamine fermentation Assays for the ability of a microorganism to ferment tryptamine. tryptamine acidification tryptophan fermentation assay tryptophan acidification tryptophan DLtryptophan tryptophan fermentation DL-tryptophan Carrine Blank tryptophane Assays for the ability of a microorganism to ferment tryptophan. L-tryptophan fermentation assay Carrine Blank L-tryptophan acidification L-tryptophane L-tryptophan fermentation Assays for the ability of a microorganism to ferment L-tryptophan. L-tryptophan tyrosine fermentation assay tyrosine tyrosin Assays for the ability of a microorganism to ferment tyrosine. tyrosine acidification Carrine Blank tyrosine fermentation TyrA L-tyrosine fermentation assay Assays for the ability of a microorganism to ferment L-tyrosine. Carrine Blank L-tyrosine acidification L-tyrosine fermentation L-tyrosine uracil fermentation assay uracil fermentation uracil Carrine Blank Assays for the ability of a microorganism to ferment uracil. uracil acidification urea fermentation assay urea acidification urea Carrine Blank urea fermentation Assays for the ability of a microorganism to ferment urea. uric acid fermentation assay Carrine Blank uric acid fermentation urate Assays for the ability of a microorganism to ferment uric acid (urate). uric acid acidification uric acid uridine fermentation assay Assays for the ability of a microorganism to ferment uridine. uridine acidification Carrine Blank uridine fermentation uridine UMP fermentation assay UMP acidification UMP fermentation Carrine Blank uridine 5'-monophosphate Assays for the ability of a microorganism to ferment UMP. UMP uridine monophosphate urocanic acid fermentation assay Carrine Blank urocanic acid urocanate Assays for the ability of a microorganism to ferment urocanate (urocanic acid). urocanate acidification urocanate fermentation valeric acid fermentation assay valerate acidification Carrine Blank n-valeric acid pentanoate Assays for the ability of a microorganism to ferment valerate (valeric acid). vaerate valerate n-valerate valerate fermentation valeric acid valine fermentation assay Assays for the ability of a microorganism to ferment valine. Carrine Blank valine valine acidification valine fermentation L-valine fermentation assay L-valine acidification Carrine Blank L-valine Assays for the ability of a microorganism to ferment L-valine. L-valine fermentation vanillic acid fermentation assay 4-hydroxy-3-methoxybenzoic acid vanillate Assays for the ability of a microorganism to ferment vanillate (vanillic acid). vanilate vanillate acidification vanillic acid vanillate fermentation 4-hydroxy-3-methoxybenzoate Carrine Blank vanillin fermentation assay vanillin acidification vanillin fermentation 4-hydroxy-3-methoxybenzaldehyde vanillin Carrine Blank Assays for the ability of a microorganism to ferment vanillin. veratraldehyde fermentation assay 4-dimethoxybenzaldehyde veratraldehyde veratraldehyde acidification Assays for the ability of a microorganism to ferment veratraldehyde. Carrine Blank veratraldehyde fermentation xanthine fermentation assay Assays for the ability of a microorganism to ferment xanthine. xanthine xanthine fermentation xanthine acidification xanthin Carrine Blank methyl mannoside fermentation assay methyl mannoside acidification Assays for the ability of a microorganism to ferment methyl mannoside. methyl mmannoside methyl mannoside fermentation methyl mannoside Carrine Blank vanillin assimilation assay 4-hydroxy-3-methoxybenzaldehyde Carrine Blank vanillin Assays for the ability of a microorganism to assimilate vanillin as a sole source of carbon and energy. vanillin assimilation vanillic acid assimilation assay Assays for the ability of a microorganism to assimilate vanillate (vanillic acid) as a sole source of carbon and energy. 4-hydroxy-3-methoxybenzoic acid vanillate vanillate assimilation Carrine Blank 4-hydroxy-3-methoxybenzoate vanilate vanillic acid valine assimilation assay Assays for the ability of a microorganism to assimilate valine as a sole source of carbon and energy. valine Carrine Blank valine assimilation uric acid assimilation assay uric acid assimilation Carrine Blank uric acid urate Assays for the ability of a microorganism to assimilate uric acid (urate) as a sole source of carbon and energy. urea assimilation assay Carrine Blank urea urea assimilation Assays for the ability of a microorganism to assimilate urea as a sole source of carbon and energy. uracil assimilation assay uracil assimilation uracil Assays for the ability of a microorganism to assimilate uracil as a sole source of carbon and energy. Carrine Blank L-tyrosine assimilation assay Carrine Blank L-tyrosine Assays for the ability of a microorganism to assimilate L-tyrosine as a sole source of carbon and energy. L-tyrosine assimilation tyrosine assimilation assay tyrosine assimilation tyrosine Assays for the ability of a microorganism to assimilate tyrosine as a sole source of carbon and energy. tyrosin Carrine Blank L-tryptophan assimilation assay L-tryptophane L-tryptophan Assays for the ability of a microorganism to assimilate L-tryptophan as a sole source of carbon and energy. Carrine Blank L-tryptophan assimilation tryptophan assimilation assay Carrine Blank tryptophan tryptophan assimilation DL-tryptophan tryptophane DLtryptophan Assays for the ability of a microorganism to assimilate tryptophan as a sole source of carbon and energy. tryptamine assimilation assay Assays for the ability of a microorganism to assimilate tryptamine as a sole source of carbon and energy. Carrine Blank tryptamine tryptamine assimilation trimethylamine assimilation assay trimethylamine assimilation trimethylamine trimethylammonium chloride trimethlamine Carrine Blank Assays for the ability of a microorganism to assimilate trimethylamine as a sole source of carbon and energy. trihydroxybenzoic acid assimilation assay trihydroxybenzoate assimilation trihydroxybenzoate trihydroxybenzoic acid Carrine Blank Assays for the ability of a microorganism to assimilate trihydroxybenzoate (trihydroxybenzoic acid) as a sole source of carbon and energy. tricarballylic acid assimilation assay Carrine Blank tricarballylate tricarballylic acid tricarballylate assimilation Assays for the ability of a microorganism to assimilate tricarballylate (tricarballylic acid) as a sole source of carbon and energy. tributyrin assimilation assay Assays for the ability of a microorganism to assimilate tributyrin as a sole source of carbon and energy. glyceryl tributyrate tributyrin tributyrin assimilation tributyrate Carrine Blank trehalose assimilation assay trehalose tregalose Carrine Blank trehalose assimilation treharose Assays for the ability of a microorganism to assimilate trehalose as a sole source of carbon and energy. threhalose xylose assimilation assay DL-xylose Assays for the ability of a microorganism to assimilate xylose as a sole source of carbon and energy. xylose D- and L-xylose D or Lxylose xylose assimilation Carrine Blank D- or L-xylose methyl mannoside assimilation assay methyl-mmannoside Assays for the ability of a microorganism to assimilate methyl mannoside as a sole source of carbon and energy. Carrine Blank methyl mannoside assimilation methyl mannoside methyl galactoside assimilation assay methyl galactoside Assays for the ability of a microorganism to assimilate methyl galactoside as a sole source of carbon and energy. methyl galactoside assimilation Carrine Blank N-acetylgalactosamine assimilation assay Carrine Blank N-acetyl-galactosamine Assays for the ability of a microorganism to assimilate N-acetylgalactosamine as a sole source of carbon and energy. N-acetylgalactosamine N-acetylgalactosamine assimilation N-acetylmannosamine assimilation assay N-acetylmannosamine assimilation Assays for the ability of a microorganism to assimilate N-acetylmannosamine as a sole source of carbon and energy. Carrine Blank N-acetylmannosamine N-acetyl-D-glucosamine assimilation assay Assays for the ability of a microorganism to assimilate N-acetyl-D-glucosamine as a sole source of carbon and energy. N-acetyl-Dglucosamine N-acetyl-D-glucosamine N-acetyl-D-glucosamine assimilation Carrine Blank NAG N-acetyl-D-galactosamine assimilation assay Assays for the ability of a microorganism to assimilate N-acetyl-D-galactosamine as a sole source of carbon and energy. N-acetyl-D-galactosamine N-acetyl-D-galactosamine assimilation Carrine Blank N-acetyl-Dgalactosamine alpha-D-glucose assimilation assay Assays for the ability of a microorganism to assimilate alpha-D-glucose as a sole source of carbon and energy. a-Dglucose alpha-D-glucose assimilation a-D-glucose alpha-D-glucose Carrine Blank xylene fermentation assay xylene xylene fermentation Carrine Blank Assays for the ability of a microorganism to ferment xylene. xylene acidification xylooligosaccharide fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment xylooligosaccharide. xylooligosaccharide xylooligosaccharide acidification xylooligosaccharide fermentation xylooligosaccharides xylose fermentation assay Assays for the ability of a microorganism to ferment xylose. D- or L-xylose D- and L-xylose DL-xylose D or Lxylose xylose acidification Carrine Blank xylose xylose fermentation methyl galactoside fermentation assay Assays for the ability of a microorganism to ferment methyl galactoside. methyl galactoside methyl galactoside acidification Carrine Blank methyl galactoside fermentation N-acetylgalactosamine fermentation assay N-acetyl-galactosamine N-acetylgalactosamine fermentation Assays for the ability of a microorganism to ferment N-acetylgalactosamine. N-acetylgalactosamine acidification N-acetylgalactosamine Carrine Blank N-acetylmannosamine fermentation assay N-acetylmannosamine acidification Assays for the ability of a microorganism to ferment N-acetylmannosamine. Carrine Blank N-acetylmannosamine fermentation N-acetylmannosamine N-acetyl-D-glucosamine fermentation assay Carrine Blank N-acetyl-D-glucosamine fermentation Assays for the ability of a microorganism to ferment N-acetyl-D-glucosamine. N-acetyl-D-glucosamine acidification NAG N-acetyl-D-glucosamine N-acetyl-Dglucosamine N-acetyl-D-galactosamine fermentation assay Carrine Blank N-acetyl-D-galactosamine fermentation N-acetyl-Dgalactosamine N-acetyl-D-galactosamine acidification N-acetyl-D-galactosamine Assays for the ability of a microorganism to ferment N-acetyl-D-galactosamine. L-mannitol fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment L-mannitol. L-mannitol fermentation L-mannitol acidification L-mannitol alpha-D-mannoside fermentation assay Assays for the ability of a microorganism to ferment alpha-D-mannoside. alpha-D-mannoside acidification a-mannopyranoside alpha-D-mannoside alpha-D-mannoside fermentation Carrine Blank glycerol monophosphate fermentation assay glycerol monophosphate acidification glycerol monophosphate glycerol monophosphate fermentation Carrine Blank Assays for the ability of a microorganism to ferment glycerol monophosphate. D-galactono-1,4-lactone fermentation assay Carrine Blank D-galactono-1,4-lactone D-galactono-1,4-lactone acidification D-galactono-1,4-lactone fermentation D-galacutronic acid lactone Assays for the ability of a microorganism to ferment D-galactono-1,4-lactone. glucuronolactone fermentation assay gamma-glucuronic acid glucuronolactone acidification Carrine Blank glucuronolactone fermentation Assays for the ability of a microorganism to ferment glucuronolactone. glucuronolactone alpha-D-glucose fermentation assay a-D-glucose Assays for the ability of a microorganism to ferment alpha-D-glucose. a-Dglucose alpha-D-glucose alpha-D-glucose fermentation alpha-D-glucose acidification Carrine Blank L-arabinitol fermentation assay L-arabinitol acidification Larabitol L-arabinitol L-arabitol Carrine Blank L-arabinitol fermentation Assays for the ability of a microorganism to ferment L-arabinitol. trans-caffeic acid assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate trans-caffeate as a sole source of carbon and energy. trans-caffeate trans-caffeate assimilation cis-caffeic acid assimilation assay cis-caffeate Carrine Blank cis-caffeate assimilation Assays for the ability of a microorganism to assimilate cis-caffeate as a sole source of carbon and energy. D-arabinose assimilation assay Assays for the ability of a microorganism to assimilate D-arabinose as a sole source of carbon and energy. Carrine Blank D-arabinose assimilation Darabinose D-arabinose L-arabinitol assimilation assay L-arabitol Larabitol L-arabinitol L-arabinitol assimilation Carrine Blank Assays for the ability of a microorganism to assimilate L-arabinitol as a sole source of carbon and energy. alpha-D-glucose 6-phosphate assimilation assay alpha-D-glucose 6-phosphate assimilation a-D-glucose 6-phosphate a-D-glucose 6-PO4 Carrine Blank alpha-D-glucose 6-phosphate Assays for the ability of a microorganism to assimilate alpha-D-glucose 6-phosphate as a sole source of carbon and energy. alpha-D-glucose 6-PO4 (R)-lactic acid assimilation assay (R)-lactate assimilation D-lactic acid Carrine Blank D-lactate (R)-lactate Dlactic acid Assays for the ability of a microorganism to assimilate (R)-lactate as a sole source of carbon and energy. L-lyxose assimilation assay Assays for the ability of a microorganism to assimilate L-lyxose as a sole source of carbon and energy. L-lyxose Carrine Blank L-lyxose assimilation L-mannitol assimilation assay Assays for the ability of a microorganism to assimilate L-mannitol as a sole source of carbon and energy. L-mannitol assimilation L-mannitol Carrine Blank alpha-D-mannoside assimilation assay alpha-D-mannoside assimilation a-mannopyranoside Carrine Blank alpha-D-mannoside Assays for the ability of a microorganism to assimilate alpha-D-mannoside as a sole source of carbon and energy. glycerol monophosphate assimilation assay glycerol monophosphate Assays for the ability of a microorganism to assimilate glycerol monophosphate as a sole source of carbon and energy. L-a-glycerol phosphate glycerol monophosphate assimilation Carrine Blank D-galacturonic acid assimilation assay D-galacturonic acid dGATa D-galacturonic acid assimilation Carrine Blank Assays for the ability of a microorganism to assimilate D-galacturonic acid (D-galacturonate) as a sole source of carbon and energy. Dgalacturonate D-galacturonate L-glucitol assimilation assay Carrine Blank L-glucitol L-sorbitol L-glucitol assimilation Assays for the ability of a microorganism to assimilate L-glucitol as a sole source of carbon and energy. D-glucosamine assimilation assay D-glucosamine assimilation D-glucosamine Carrine Blank Assays for the ability of a microorganism to assimilate D-glucosamine as a sole source of carbon and energy. beta-D-glucosamine assimilation assay beta-D-glucosamine assimilation b-glucosamine Carrine Blank Assays for the ability of a microorganism to assimilate beta-D-glucosamine as a sole source of carbon and energy. beta-D-glucosamine alpha-glucoside assimilation assay alpha-glucoside alpha-glucoside assimilation Assays for the ability of a microorganism to assimilate alpha-glucoside as a sole source of carbon and energy. a-glucopyranoside Carrine Blank beta-glucoside assimilation assay Carrine Blank beta-glucoside assimilation Assays for the ability of a microorganism to assimilate beta-glucoside as a sole source of carbon and energy. b-glucopyranoside beta-glucoside glucuronolactone assimilation assay Assays for the ability of a microorganism to assimilate glucuronolactone as a sole source of carbon and energy. Carrine Blank glucuronolactone glucuronolactone assimilation gamma-glucuronic acid D-galactono-1,5-lactone fermentation assay D-galactono-1,5-lactone fermentation Assays for the ability of a microorganism to ferment D-galactono-1,5-lactone. D-galactono-1,5-lactone D-galactono-1,5-lactone acidification D-galacutronic acid lactone Carrine Blank monounsaturated fatty acid fermentation assay Assays for the ability of a microorganism to ferment monounsaturated fatty acid. Carrine Blank monounsaturated fatty acid acidification monounsaturated fatty acid monounsaturated fatty acid fermentation beta-D-fucose fermentation assay b-fucose beta-D-fucose fermentation beta-D-fucose acidification Carrine Blank beta-D-fucose Assays for the ability of a microorganism to ferment beta-D-fucose. beta-fucose beta-L-fucose fermentation assay beta-L-fucose acidification Carrine Blank beta-L-fucose fermentation b-fucose Assays for the ability of a microorganism to ferment beta-L-fucose. beta-fucose beta-L-fucose alpha-L-fucose fermentation assay a-L-fucose Assays for the ability of a microorganism to ferment alpha-L-fucose. alpha-L-fucose acidification alpha-L-fucose alpha-L-fucose fermentation Carrine Blank D-galacturonic acid fermentation assay Carrine Blank D-galacturonic acid acidification Assays for the ability of a microorganism to ferment D-galacturonic acid (D-galacturonate). D-galacturonic acid D-galacturonic acid fermentation D-galacturonate Dgalacturonate L-glucitol fermentation assay Carrine Blank L-glucitol acidification L-glucitol fermentation Assays for the ability of a microorganism to ferment L-glucitol. L-glucitol L-sorbitol D-glucosamine fermentation assay D-glucosamine D-glucosamine fermentation D-glucosamine acidification Carrine Blank Assays for the ability of a microorganism to ferment D-glucosamine. beta-D-glucosamine fermentation assay b-glucosamine beta-D-glucosamine acidification beta-D-glucosamine fermentation beta-D-glucosamine Assays for the ability of a microorganism to ferment beta-D-glucosamine. Carrine Blank alpha-glucoside fermentation assay a-glucopyranoside alpha-glucoside fermentation alpha-glucoside acidification Assays for the ability of a microorganism to ferment alpha-glucoside. Carrine Blank alpha-glucoside alpha-glucopyranoside beta-glucoside fermentation assay Carrine Blank beta-glucoside fermentation Assays for the ability of a microorganism to ferment beta-glucoside. beta-glucoside b-glucopyranoside beta-glucoside acidification fatty acid assimilation assay fatty acid fatty acid assimilation Carrine Blank Assays for the ability of a microorganism to assimilate fatty acid as a sole source of carbon and energy. L-citrulline assimilation assay Assays for the ability of a microorganism to assimilate L-citrulline as a sole source of carbon and energy. L-citrulline assimilation L-citrulline Carrine Blank L-citruline beta-D-fructofuranose assimilation assay beta-D-fructose Assays for the ability of a microorganism to assimilate beta-D-fructofuranose as a sole source of carbon and energy. b-D-fructose beta-D-fructofuranose beta-D-fructofuranose assimilation Carrine Blank bdfructose alpha-D-fructofuranose assimilation assay Assays for the ability of a microorganism to assimilate alpha-D-fructofuranose as a sole source of carbon and energy. Carrine Blank alpha-D-fructose a-D-fructose alpha-D-fructofuranose assimilation alpha-D-fructofuranose L-fructose assimilation assay Carrine Blank L-fructose L-fructose assimilation Assays for the ability of a microorganism to assimilate L-fructose as a sole source of carbon and energy. beta-D-fucose assimilation assay beta-D-fucose beta-D-fucose assimilation beta-fucose b-fucose Carrine Blank Assays for the ability of a microorganism to assimilate beta-D-fucose as a sole source of carbon and energy. beta-L-fucose assimilation assay b-fucose beta-L-fucose assimilation Assays for the ability of a microorganism to assimilate beta-L-fucose as a sole source of carbon and energy. beta-fucose beta-L-fucose Carrine Blank alpha-L-fucose assimilation assay alpha-L-fucose Assays for the ability of a microorganism to assimilate alpha-L-fucose as a sole source of carbon and energy. Carrine Blank alpha-L-fucose assimilation a-L-fucose D-galactono-1,5-lactone assimilation assay D-galactono-1,5-lactone assimilation D-galacutronic acid lactone Assays for the ability of a microorganism to assimilate D-galactono-1,5-lactone as a sole source of carbon and energy. D-galactono-1,5-lactone Carrine Blank D-galactono-1,4-lactone assimilation assay D-galactono-1,4-lactone assimilation Carrine Blank D-galacutronic acid lactone D-galactono-1,4-lactone Assays for the ability of a microorganism to assimilate D-galactono-1,4-lactone as a sole source of carbon and energy. L-fructose fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment L-fructose. L-fructose L-fructose fermentation L-fructose acidification alpha-D-fructofuranose fermentation assay alpha-D-fructofuranose acidification alpha-D-fructose Carrine Blank alpha-D-fructofuranose a-D-fructose alpha-D-fructofuranose fermentation Assays for the ability of a microorganism to ferment alpha-D-fructofuranose. beta-D-fructofuranose fermentation assay beta-D-fructofuranose acidification beta-D-fructofuranose fermentation beta-D-fructofuranose bdfructose Assays for the ability of a microorganism to ferment beta-D-fructofuranose. beta-D-fructose b-D-fructose Carrine Blank L-citrulline fermentation assay L-citrulline L-citrulline acidification Assays for the ability of a microorganism to ferment L-citrulline. L-citruline Carrine Blank L-citrulline fermentation creatinine fermentation assay L-creatinine Carrine Blank creatinine Assays for the ability of a microorganism to ferment creatinine. creatinine fermentation creatinine acidification long-chain fatty acid fermentation assay long-chain fatty acid Carrine Blank long-chain fatty acid fermentation Assays for the ability of a microorganism to ferment long-chain fatty acid. long-chain fatty acid acidification branched-chain fatty acid fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment branched-chain fatty acid. branched-chain fatty acid fermentation branched-chain fatty acid branched-chain fatty acid acidification hydroxy fatty acid fermentation assay hydroxy fatty acid acidification Assays for the ability of a microorganism to ferment hydroxy fatty acid. hydroxy fatty acid hydroxy fatty acid fermentation Carrine Blank medium-chain fatty acid fermentation assay Carrine Blank medium-chain fatty acid fermentation medium-chain fatty acid medium-chain fatty acid acidification Assays for the ability of a microorganism to ferment medium-chain fatty acid. short-chain fatty acid fermentation assay short-chain fatty acid acidification short-chain fatty acid fermentation Assays for the ability of a microorganism to ferment short-chain fatty acid. Carrine Blank short-chain fatty acid straight-chain fatty acid fermentation assay Assays for the ability of a microorganism to ferment straight-chain fatty acid. Carrine Blank straight-chain fatty acid fermentation straight-chain fatty acid acidification straight-chain fatty acid unsaturated fatty acid fermentation assay Carrine Blank unsaturated fatty acid fermentation unsaturated fatty acid Assays for the ability of a microorganism to ferment unsaturated fatty acid. unsaturated fatty acid acidification fatty acid fermentation assay Assays for the ability of a microorganism to ferment fatty acid. fatty acid acidification fatty acid Carrine Blank fatty acid fermentation methyl-branched fatty acid fermentation assay methyl-branched fatty acid acidification Assays for the ability of a microorganism to ferment methyl-branched fatty acid. methyl-branched fatty acid fermentation Carrine Blank methyl-branched fatty acid cyclic fatty acid fermentation assay Assays for the ability of a microorganism to ferment cyclic fatty acid. cyclic fatty acid fermentation cyclic fatty acid acidification Carrine Blank cyclic fatty acid cyclopropeny fatty acid fermentation assay Carrine Blank cyclopropeny fatty acid acidification cyclopropeny fatty acid fermentation Assays for the ability of a microorganism to ferment cyclopropeny fatty acid. cyclopropeny fatty acid nitrogen-containing fatty acid fermentation assay nitrogen-containing fatty acid fermentation nitrogen-containing fatty acid acidification Carrine Blank Assays for the ability of a microorganism to ferment nitrogen-containing fatty acid. nitrogen-containing fatty acid L-asparagine fermentation assay L-asparagine fermentation Carrine Blank L-asparagine acidification Assays for the ability of a microorganism to ferment L-asparagine. L-asparagine unsaturated fatty acid assimilation assay unsaturated fatty acid Carrine Blank Assays for the ability of a microorganism to assimilate unsaturated fatty acid as a sole source of carbon and energy. unsaturated fatty acid assimilation straight-chain fatty acid assimilation assay Carrine Blank straight-chain fatty acid assimilation straight-chain fatty acid Assays for the ability of a microorganism to assimilate straight-chain fatty acid as a sole source of carbon and energy. short-chain fatty acid assimilation assay short-chain fatty acid Carrine Blank short-chain fatty acid assimilation Assays for the ability of a microorganism to assimilate short-chain fatty acid as a sole source of carbon and energy. medium-chain fatty acid assimilation assay Carrine Blank medium-chain fatty acid medium-chain fatty acid assimilation Assays for the ability of a microorganism to assimilate medium-chain fatty acid as a sole source of carbon and energy. hydroxy fatty acid assimilation assay hydroxy fatty acid assimilation Carrine Blank hydroxy fatty acid Assays for the ability of a microorganism to assimilate hydroxy fatty acid as a sole source of carbon and energy. branched-chain fatty acid assimilation assay Carrine Blank branched-chain fatty acid Assays for the ability of a microorganism to assimilate branched-chain fatty acid as a sole source of carbon and energy. branched-chain fatty acid assimilation long-chain fatty acid assimilation assay Assays for the ability of a microorganism to assimilate long-chain fatty acid as a sole source of carbon and energy. Carrine Blank long-chain fatty acid assimilation long-chain fatty acid monounsaturated fatty acid assimilation assay monounsaturated fatty acid assimilation monounsaturated fatty acid Carrine Blank Assays for the ability of a microorganism to assimilate monounsaturated fatty acid as a sole source of carbon and energy. methyl-branched fatty acid assimilation assay Carrine Blank methyl-branched fatty acid assimilation methyl-branched fatty acid Assays for the ability of a microorganism to assimilate methyl-branched fatty acid as a sole source of carbon and energy. cyclic fatty acid assimilation assay cyclic fatty acid assimilation Assays for the ability of a microorganism to assimilate cyclic fatty acid as a sole source of carbon and energy. cyclic fatty acid Carrine Blank cyclopropeny fatty acid assimilation assay Assays for the ability of a microorganism to assimilate cyclopropeny fatty acid as a sole source of carbon and energy. Carrine Blank cyclopropeny fatty acid cyclopropeny fatty acid assimilation nitrogen-containing fatty acid assimilation assay nitrogen-containing fatty acid Carrine Blank Assays for the ability of a microorganism to assimilate nitrogen-containing fatty acid as a sole source of carbon and energy. nitrogen-containing fatty acid assimilation oxo fatty acid assimilation assay Carrine Blank oxo fatty acid assimilation oxo fatty acid Assays for the ability of a microorganism to assimilate oxo fatty acid as a sole source of carbon and energy. saturated fatty acid assimilation assay saturated fatty acid assimilation saturated fatty acid Carrine Blank Assays for the ability of a microorganism to assimilate saturated fatty acid as a sole source of carbon and energy. methylbutyric acid assimilation assay methylbutyric acid Carrine Blank Assays for the ability of a microorganism to assimilate methylbutyric acid as a sole source of carbon and energy. methylbutyric acid assimilation lipoic acid assimilation assay Assays for the ability of a microorganism to assimilate lipoic acid as a sole source of carbon and energy. lipoic acid lipoic acid assimilation Carrine Blank straight-chain saturated fatty acid assimilation assay straight-chain saturated fatty acid assimilation Assays for the ability of a microorganism to assimilate straight-chain saturated fatty acid as a sole source of carbon and energy. Carrine Blank straight-chain saturated fatty acid oxo fatty acid fermentation assay Assays for the ability of a microorganism to ferment oxo fatty acid. oxo fatty acid acidification Carrine Blank oxo fatty acid fermentation oxo fatty acid saturated fatty acid fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment saturated fatty acid. saturated fatty acid acidification saturated fatty acid fermentation saturated fatty acid methylbutyric acid fermentation assay methylbutyric acid fermentation Assays for the ability of a microorganism to ferment methylbutyric acid. methylbutyric acid acidification Carrine Blank methylbutyric acid lipoic acid fermentation assay lipoic acid fermentation Carrine Blank Assays for the ability of a microorganism to ferment lipoic acid. lipoic acid acidification lipoic acid carbohydrate fermentation assay carbohydrate fermentation Assays for the ability of a microorganism to ferment carbohydrate. carbohydrate acidification Carrine Blank carbohydrate alditol fermentation assay alditol Assays for the ability of a microorganism to ferment alditol. alditol fermentation alditol acidification Carrine Blank hexitol fermentation assay hexitol hexitol fermentation Carrine Blank Assays for the ability of a microorganism to ferment hexitol. hexitol acidification pentitol fermentation assay Assays for the ability of a microorganism to ferment pentitol. Carrine Blank pentitol acidification pentitol pentitol fermentation tetritol fermentation assay tetritol Assays for the ability of a microorganism to ferment tetritol. tetritol acidification Carrine Blank tetritol fermentation polysaccharide derivative fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment polysaccharide derivative. polysaccharide derivative fermentation polysaccharide derivative acidification polysaccharide derivative methyl glycoside fermentation assay methyl glycoside fermentation Assays for the ability of a microorganism to ferment methyl glycoside. methyl glycoside acidification methyl glycoside Carrine Blank straight-chain saturated fatty acid fermentation assay straight-chain saturated fatty acid fermentation straight-chain saturated fatty acid Carrine Blank Assays for the ability of a microorganism to ferment straight-chain saturated fatty acid. straight-chain saturated fatty acid acidification carbohydrate assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate carbohydrate as a sole source of carbon and energy. carbohydrate carbohydrate assimilation alditol assimilation assay Carrine Blank alditol assimilation alditol Assays for the ability of a microorganism to assimilate alditol as a sole source of carbon and energy. hexitol assimilation assay Assays for the ability of a microorganism to assimilate hexitol as a sole source of carbon and energy. hexitol Carrine Blank hexitol assimilation pentitol assimilation assay Assays for the ability of a microorganism to assimilate pentitol as a sole source of carbon and energy. pentitol assimilation pentitol Carrine Blank tetritol assimilation assay Carrine Blank tetritol tetritol assimilation Assays for the ability of a microorganism to assimilate tetritol as a sole source of carbon and energy. aromatic ether assimilation assay aromatic ether Carrine Blank aromatic ether assimilation Assays for the ability of a microorganism to assimilate aromatic ether as a sole source of carbon and energy. dimethoxybenzene assimilation assay Carrine Blank dimethoxybenzene dimethoxybenzene assimilation Assays for the ability of a microorganism to assimilate dimethoxybenzene as a sole source of carbon and energy. monomethoxybenzene assimilation assay monomethoxybenzene assimilation Assays for the ability of a microorganism to assimilate monomethoxybenzene as a sole source of carbon and energy. Carrine Blank monomethoxybenzene carbohydrate acid assimilation assay Assays for the ability of a microorganism to assimilate carbohydrate acid as a sole source of carbon and energy. carbohydrate acid carbohydrate acid assimilation Carrine Blank aldaric acid assimilation assay Carrine Blank aldaric acid Assays for the ability of a microorganism to assimilate aldaric acid as a sole source of carbon and energy. aldaric acid assimilation soluble starch fermentation assay soluble starch fermentation soluble starch acidification Assays for the ability of a microorganism to ferment soluble starch. soluble starch Carrine Blank soluble potato starch polysaccharide fermentation assay polysaccharide fermentation Carrine Blank polysaccharide acidification polysaccharide Assays for the ability of a microorganism to ferment polysaccharide. trisaccharide fermentation assay trisaccharide Assays for the ability of a microorganism to ferment trisaccharide. trisaccharide fermentation Carrine Blank trisaccharide acidification raffinose family oligosaccharide fermentation assay Assays for the ability of a microorganism to ferment raffinose family oligosaccharide. raffinose family oligosaccharide raffinose family oligosaccharide fermentation raffinose family oligosaccharide acidification Carrine Blank maltooligosaccharide fermentation assay maltooligosaccharide Assays for the ability of a microorganism to ferment maltooligosaccharide. Carrine Blank maltooligosaccharide acidification maltooligosaccharidex fermentation disaccharide fermentation assay Assays for the ability of a microorganism to ferment disaccharide. disaccharide disaccharide acidification disaccharide fermentation Carrine Blank cyclic oligosaccharide fermentation assay Assays for the ability of a microorganism to ferment cyclic oligosaccharide. cyclic oligosaccharide fermentation Carrine Blank cyclic oligosaccharide acidification cyclic oligosaccharide oligosaccharide fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment oligosaccharide. oligosaccharide oligosaccharide fermentation oligosaccharide acidification diketoaldonic acid fermentation assay diketoaldonic acid fermentation diketoaldonic acid diketoaldonic acid acidification Carrine Blank Assays for the ability of a microorganism to ferment diketoaldonic acid. deoxy sugar fermentation assay Assays for the ability of a microorganism to ferment deoxy sugar. deoxy sugar acidification deoxy sugar Carrine Blank deoxy sugar fermentation ketoaldonic acid fermentation assay ketoaldonic acid ketoaldonic acid fermentation Carrine Blank ketoaldonic acid acidification Assays for the ability of a microorganism to ferment ketoaldonic acid. uronic acid fermentation assay Carrine Blank uronic acid uronic acid fermentation uronic acid acidification Assays for the ability of a microorganism to ferment uronic acid. hexaric acid fermentation assay hexaric acid fermentation hexaric acid hexaric acid acidification Carrine Blank Assays for the ability of a microorganism to ferment hexaric acid. tetraric acid fermentation assay tetraric acid acidification Carrine Blank tetraric acid tetraric acid fermentation Assays for the ability of a microorganism to ferment tetraric acid. hexonic acid fermentation assay hexonic acid fermentation Assays for the ability of a microorganism to ferment hexonic acid. hexonic acid Carrine Blank hexonic acid acidification aldonic acid fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment aldonic acid. aldonic acid acidification aldonic acid aldonic acid fermentation aldaric acid fermentation assay Assays for the ability of a microorganism to ferment aldaric acid. aldaric acid Carrine Blank aldaric acid fermentation aldaric acid acidification carbohydrate acid fermentation assay Assays for the ability of a microorganism to ferment carbohydrate acid. Carrine Blank carbohydrate acid carbohydrate acid acidification carbohydrate acid fermentation monomethoxybenzene fermentation assay monomethoxybenzene monomethoxybenzene acidification Assays for the ability of a microorganism to ferment monomethoxybenzene. Carrine Blank monomethoxybenzene fermentation dimethoxybenzene fermentation assay dimethoxybenzene acidification Carrine Blank dimethoxybenzene fermentation Assays for the ability of a microorganism to ferment dimethoxybenzene. dimethoxybenzene aromatic ether fermentation assay aromatic ether aromatic ether fermentation aromatic ether acidification Assays for the ability of a microorganism to ferment aromatic ether. Carrine Blank aldonic acid assimilation assay Assays for the ability of a microorganism to assimilate aldonic acid as a sole source of carbon and energy. aldonic acid assimilation aldonic acid Carrine Blank hexonic acid assimilation assay hexonic acid assimilation Carrine Blank hexanoic acid Assays for the ability of a microorganism to assimilate hexonic acid as a sole source of carbon and energy. tetraric acid assimilation assay Carrine Blank tetraric acid assimilation tetraric acid Assays for the ability of a microorganism to assimilate tetraric acid as a sole source of carbon and energy. hexaric acid assimilation assay hexaric acid assimilation hexaric acid Assays for the ability of a microorganism to assimilate hexaric acid as a sole source of carbon and energy. Carrine Blank uronic acid assimilation assay uronic acid Carrine Blank Assays for the ability of a microorganism to assimilate uronic acid as a sole source of carbon and energy. uronic acid assimilation ketoaldonic acid assimilation assay ketoaldonic acid assimilation ketoaldonic acid Assays for the ability of a microorganism to assimilate ketoaldonic acid as a sole source of carbon and energy. Carrine Blank deoxy sugar assimilation assay deoxy sugar Assays for the ability of a microorganism to assimilate deoxy sugar as a sole source of carbon and energy. Carrine Blank deoxy sugar assimilation diketoaldonic acid assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate diketoaldonic acid as a sole source of carbon and energy. diketoaldonic acid diketoaldonic acid assimilation oligosaccharide assimilation assay oligosaccharide assimilation Assays for the ability of a microorganism to assimilate oligosaccharide as a sole source of carbon and energy. oligosaccharide Carrine Blank cyclic oligosaccharide assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate cyclic oligosaccharide as a sole source of carbon and energy. cyclic oligosaccharide assimilation cyclic oligosaccharide disaccharide assimilation assay Carrine Blank disaccharide assimilation disaccharide Assays for the ability of a microorganism to assimilate disaccharide as a sole source of carbon and energy. maltooligosaccharide assimilation assay maltooligosaccharide Assays for the ability of a microorganism to assimilate maltooligosaccharide as a sole source of carbon and energy. maltooligosaccharide assimilation Carrine Blank raffinose family oligosaccharide assimilation assay raffinose family oligosaccharide assimilation Assays for the ability of a microorganism to assimilate raffinose family oligosaccharide as a sole source of carbon and energy. Carrine Blank raffinose family oligosaccharide trisaccharide assimilation assay trisaccharide assimilation trisaccharide Assays for the ability of a microorganism to assimilate trisaccharide as a sole source of carbon and energy. Carrine Blank polysaccharide assimilation assay Assays for the ability of a microorganism to assimilate polysaccharide as a sole source of carbon and energy. polysaccharide polysaccharide assimilation Carrine Blank inulobiose assimilation assay inulobiose assimilation Carrine Blank Assays for the ability of a microorganism to assimilate inulobiose as a sole source of carbon and energy. inulobiose soluble starch assimilation assay soluble starch Assays for the ability of a microorganism to assimilate soluble starch as a sole source of carbon and energy. soluble potato starch soluble starch assimilation Carrine Blank monosaccharide assimilation assay Assays for the ability of a microorganism to assimilate monosaccharide as a sole source of carbon and energy. monosaccharide Carrine Blank monosaccharide assimilation aldohexose assimilation assay Assays for the ability of a microorganism to assimilate aldohexose as a sole source of carbon and energy. aldohexose aldohexose assimilation Carrine Blank aldose assimilation assay aldose Carrine Blank aldose assimilation Assays for the ability of a microorganism to assimilate aldose as a sole source of carbon and energy. aldotetrose assimilation assay Assays for the ability of a microorganism to assimilate aldotetrose as a sole source of carbon and energy. aldotetrose aldotetrose assimilation Carrine Blank ahydro sugar assimilation assay ahydro sugar assimilation ahydro sugar Assays for the ability of a microorganism to assimilate ahydro sugar as a sole source of carbon and energy. Carrine Blank hexose assimilation assay Carrine Blank hexose Assays for the ability of a microorganism to assimilate hexose as a sole source of carbon and energy. hexose assimilation pentose assimilation assay Assays for the ability of a microorganism to assimilate pentose as a sole source of carbon and energy. pentose Carrine Blank pentose assimilation carbohydrate derivative assimilation assay Assays for the ability of a microorganism to assimilate carbohydrate derivative as a sole source of carbon and energy. carbohydrate derivative Carrine Blank carbohydrate derivative assimilation alditol phosphate assimilation assay alditol phosphate alditol phosphate assimilation Assays for the ability of a microorganism to assimilate alditol phosphate as a sole source of carbon and energy. Carrine Blank amino sugar assimilation assay amino sugar assimilation Carrine Blank Assays for the ability of a microorganism to assimilate amino sugar as a sole source of carbon and energy. amino sugar carbohydrate acid derivative assimilation assay carbohydrate acid derivative carbohydrate acid derivative assimilation Assays for the ability of a microorganism to assimilate carbohydrate acid derivative as a sole source of carbon and energy. Carrine Blank carbohydrate lactone assimilation assay carbohydrate lactone assimilation carbohydrate lactone Assays for the ability of a microorganism to assimilate carbohydrate lactone as a sole source of carbon and energy. Carrine Blank carbohydrate phosphate assimilation assay carbohydrate phosphate assimilation Carrine Blank carbohydrate phosphate Assays for the ability of a microorganism to assimilate carbohydrate phosphate as a sole source of carbon and energy. glycoside assimilation assay Carrine Blank glycoside assimilation Assays for the ability of a microorganism to assimilate glycoside as a sole source of carbon and energy. glycoside disaccharide derivative assimilation assay disaccharide derivative assimilation disaccharide derivative Carrine Blank Assays for the ability of a microorganism to assimilate disaccharide derivative as a sole source of carbon and energy. monosaccharide derivative assimilation assay Assays for the ability of a microorganism to assimilate monosaccharide derivative as a sole source of carbon and energy. monosaccharide derivative assimilation monosaccharide derivative Carrine Blank disaccharide derivative fermentation assay Carrine Blank disaccharide derivative fermentation disaccharide derivative Assays for the ability of a microorganism to ferment disaccharide derivative. disaccharide derivative acidification glycoside fermentation assay Assays for the ability of a microorganism to ferment glycoside. glycoside fermentation Carrine Blank glycoside glycoside acidification carbohydrate phosphate fermentation assay carbohydrate phosphate fermentation carbohydrate phosphate Carrine Blank carbohydrate phosphate acidification Assays for the ability of a microorganism to ferment carbohydrate phosphate. carbohydrate lactone fermentation assay carbohydrate lactone acidification carbohydrate lactone Carrine Blank carbohydrate lactone fermentation Assays for the ability of a microorganism to ferment carbohydrate lactone. carbohydrate acid derivative fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment carbohydrate acid derivative. carbohydrate acid derivative acidification carbohydrate acid derivative carbohydrate acid derivative fermentation amino sugar fermentation assay amino sugar acidification Carrine Blank Assays for the ability of a microorganism to ferment amino sugar. amino sugar fermentation amino sugar alditol phosphate fermentation assay Assays for the ability of a microorganism to ferment alditol phosphate. Carrine Blank alditol phosphate acidification alditol phosphate fermentation alditol phosphate carbohydrate derivative fermentation assay carbohydrate derivative Assays for the ability of a microorganism to ferment carbohydrate derivative. carbohydrate derivative fermentation carbohydrate derivative acidification Carrine Blank pentose fermentation assay Carrine Blank pentose acidification pentose fermentation Assays for the ability of a microorganism to ferment pentose. pentose hexose fermentation assay hexose acidification hexose Assays for the ability of a microorganism to ferment hexose. hexose fermentation Carrine Blank ahydro sugar fermentation assay Carrine Blank ahydro sugar fermentation Assays for the ability of a microorganism to ferment ahydro sugar. ahydro sugar acidification ahydro sugar aldotetrose fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment aldotetrose. aldotetrose fermentation aldotetrose acidification aldotetrose aldose fermentation assay Assays for the ability of a microorganism to ferment aldose. Carrine Blank aldose aldose acidification aldose fermentation aldohexose fermentation assay aldohexose Assays for the ability of a microorganism to ferment aldohexose. aldohexose acidification aldohexose fermentation Carrine Blank monosaccharide fermentation assay monosaccharide acidification monosaccharide monosaccharide fermentation Carrine Blank Assays for the ability of a microorganism to ferment monosaccharide. methyl glycoside assimilation assay Carrine Blank methyl glycoside Assays for the ability of a microorganism to assimilate methyl glycoside as a sole source of carbon and energy. methyl glycoside assimilation hexoside assimilation assay Assays for the ability of a microorganism to assimilate hexoside as a sole source of carbon and energy. hexoside Carrine Blank hexoside assimilation galactoside assimilation assay Carrine Blank galactoside assimilation galactoside Assays for the ability of a microorganism to assimilate galactoside as a sole source of carbon and energy. polysaccharide derivative assimilation assay Assays for the ability of a microorganism to assimilate polysaccharide derivative as a sole source of carbon and energy. Carrine Blank polysaccharide derivative assimilation polysaccharide derivative aminoglycan assimilation assay aminoglycan Carrine Blank Assays for the ability of a microorganism to assimilate aminoglycan as a sole source of carbon and energy. aminoglycan assimilation carbohydrate sulfate assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate carbohydrate sulfate as a sole source of carbon and energy. carbohydrate sulfate carbohydrate sulfate assimilation nucleobase assimilation assay nucleobase assimilation Assays for the ability of a microorganism to assimilate nucleobase as a sole source of carbon and energy. nucleobase Carrine Blank quinolines assimilation assay Carrine Blank quinolines quinolines assimilation Assays for the ability of a microorganism to assimilate quinolines as a sole source of carbon and energy. proteinogenic amino acid assimilation assay Assays for the ability of a microorganism to assimilate proteinogenic amino acid as a sole source of carbon and energy. Carrine Blank proteinogenic amino acid assimilation proteinogenic amino acid organonitrogen compound assimilation assay Carrine Blank organonitrogen compound assimilation Assays for the ability of a microorganism to assimilate organonitrogen compound as a sole source of carbon and energy. organonitrogen compound monosaccharide derivative fermentation assay monosaccharide derivative monosaccharide derivative fermentation Assays for the ability of a microorganism to ferment monosaccharide derivative. monosaccharide derivative acidification Carrine Blank aminoglycan fermentation assay aminoglycan fermentation Assays for the ability of a microorganism to ferment aminoglycan. aminoglycan aminoglycan acidification Carrine Blank carbohydrate sulfate fermentation assay carbohydrate sulfate fermentation carbohydrate sulfate acidification carbohydrate sulfate Assays for the ability of a microorganism to ferment carbohydrate sulfate. Carrine Blank organonitrogen compound fermentation assay Assays for the ability of a microorganism to ferment organonitrogen compound. organonitrogen compound Carrine Blank organonitrogen compound fermentation organonitrogen compound acidification proteinogenic amino acid fermentation assay proteinogenic amino acid Assays for the ability of a microorganism to ferment proteinogenic amino acid. Carrine Blank proteinogenic amino acid acidification proteinogenic amino acid fermentation quinolines fermentation assay quinolines Carrine Blank quinolines acidification Assays for the ability of a microorganism to ferment quinolines. quinolines fermentation nucleobase fermentation assay Carrine Blank nucleobase fermentation nucleobase acidification nucleobase Assays for the ability of a microorganism to ferment nucleobase. lactam fermentation assay lactam Carrine Blank lactam acidification Assays for the ability of a microorganism to ferment lactam. lactam fermentation galactoside fermentation assay galactoside fermentation galactoside galactoside acidification Assays for the ability of a microorganism to ferment galactoside. Carrine Blank hexoside fermentation assay hexoside fermentation hexoside Assays for the ability of a microorganism to ferment hexoside. Carrine Blank hexoside acidification lactam assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate lactam as a sole source of carbon and energy. lactam lactam assimilation lactam assimilation assay lactam assimilation Assays for the ability of a microorganism to assimilate lactam as a sole source of carbon and energy. Carrine Blank lactam pyrimidines assimilation assay Assays for the ability of a microorganism to assimilate pyrimidines as a sole source of carbon and energy. Carrine Blank pyrimidines pyrimidines assimilation amino acid assimilation assay Assays for the ability of a microorganism to assimilate amino acid as a sole source of carbon and energy. amino acid assimilation amino acid Carrine Blank carboxylic acid assimilation assay carboxylic acid assimilation Carrine Blank carboxylic acid Assays for the ability of a microorganism to assimilate carboxylic acid as a sole source of carbon and energy. organosulfur compound assimilation assay organosulfur compound Assays for the ability of a microorganism to assimilate organosulfur compound as a sole source of carbon and energy. Carrine Blank organosulfur compound assimilation organochlorine compound assimilation assay Assays for the ability of a microorganism to assimilate organochlorine compound as a sole source of carbon and energy. organochlorine compound assimilation Carrine Blank organochlorine compound benzenes assimilation assay Carrine Blank benzenes assimilation Assays for the ability of a microorganism to assimilate benzenes as a sole source of carbon and energy. benzenes phenols assimilation assay phenols phenols assimilation Assays for the ability of a microorganism to assimilate phenols as a sole source of carbon and energy. Carrine Blank cinnamic acids assimilation assay Assays for the ability of a microorganism to assimilate cinnamic acids as a sole source of carbon and energy. cinnamic acids assimilation Carrine Blank cinnamic acids polyol assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate polyol as a sole source of carbon and energy. polyol assimilation polyol alcohol assimilation assay alcohol assimilation Assays for the ability of a microorganism to assimilate alcohol as a sole source of carbon and energy. alcohol Carrine Blank lipid assimilation assay lipid lipid assimilation Carrine Blank Assays for the ability of a microorganism to assimilate lipid as a sole source of carbon and energy. benzoic acids assimilation assay Assays for the ability of a microorganism to assimilate benzoic acids as a sole source of carbon and energy. benzoic acids benzoic acids assimilation Carrine Blank amino acid derivative assimilation assay amino acid derivative assimilation amino acid derivative Carrine Blank Assays for the ability of a microorganism to assimilate amino acid derivative as a sole source of carbon and energy. carboxamide assimilation assay carboxamide assimilation Carrine Blank carboxamide Assays for the ability of a microorganism to assimilate carboxamide as a sole source of carbon and energy. pyrimidines fermentation assay pyrimidines Assays for the ability of a microorganism to ferment pyrimidines. Carrine Blank pyrimidines acidification pyrimidines fermentation amino acid fermentation assay Assays for the ability of a microorganism to ferment amino acid. Carrine Blank amino acid amino acid fermentation amino acid acidification carboxylic acid fermentation assay Assays for the ability of a microorganism to ferment carboxylic acid. carboxylic acid fermentation carboxylic acid acidification Carrine Blank carboxylic acid ketone fermentation assay Assays for the ability of a microorganism to ferment ketone. ketone acidification ketone fermentation ketone Carrine Blank exopolysaccharide fermentation assay exopolysaccharide fermentation exopolysaccharide Carrine Blank exopolysaccharide acidification Assays for the ability of a microorganism to ferment exopolysaccharide. biomacromolecule fermentation assay Assays for the ability of a microorganism to ferment biomacromolecule. biomacromolecule fermentation Carrine Blank biomacromolecule biomacromolecule acidification information biomacromolecule fermentation assay information biomacromolecule fermentation Assays for the ability of a microorganism to ferment information biomacromolecule. information biomacromolecule Carrine Blank information biomacromolecule acidification phenylethylamine fermentation assay phenylethylamine fermentation Carrine Blank Assays for the ability of a microorganism to ferment phenylethylamine. phenylethylamine acidification phenylethylamine organic amino compound fermentation assay organic amino compound fermentation organic amino compound organic amino compound acidification Assays for the ability of a microorganism to ferment organic amino compound. Carrine Blank amine fermentation assay amine amine acidification Assays for the ability of a microorganism to ferment amine. Carrine Blank amine fermentation peptide fermentation assay Assays for the ability of a microorganism to ferment peptide. Carrine Blank peptide acidification peptides peptide compounds peptide peptide fermentation carboxamide fermentation assay carboxamide acidification carboxamide fermentation carboxamide Carrine Blank Assays for the ability of a microorganism to ferment carboxamide. amino acid derivative fermentation assay amino acid derivative fermentation amino acid derivative Carrine Blank Assays for the ability of a microorganism to ferment amino acid derivative. amino acid derivative acidification benzoic acids fermentation assay benzoic acids benzoic acids acidification Assays for the ability of a microorganism to ferment benzoic acids. Carrine Blank benzoic acids fermentation lipid fermentation assay Carrine Blank lipid lipid fermentation lipid acidification Assays for the ability of a microorganism to ferment lipid. alcohol fermentation assay alcohol fermentation Assays for the ability of a microorganism to ferment alcohol. alcohol Carrine Blank alcohol acidification polyol fermentation assay polyol fermentation Carrine Blank Assays for the ability of a microorganism to ferment polyol. polyol polyol acidification cinnamic acids fermentation assay cinnamic acids fermentation Assays for the ability of a microorganism to ferment cinnamic acids. Carrine Blank cinnamic acids cinnamic acids acidification phenols fermentation assay Carrine Blank phenols fermentation Assays for the ability of a microorganism to ferment phenols. phenols phenols acidification benzenes fermentation assay benzenes fermentation Assays for the ability of a microorganism to ferment benzenes. benzenes acidification Carrine Blank benzenes organochlorine compound fermentation assay organochlorine compound fermentation Carrine Blank organochlorine compound Assays for the ability of a microorganism to ferment organochlorine compound. organochlorine compound acidification organosulfur compound fermentation assay Assays for the ability of a microorganism to ferment organosulfur compound. organosulfur compound acidification Carrine Blank organosulfur compound organosulfur compound fermentation peptide assimilation assay Carrine Blank peptide compounds peptides peptide assimilation peptide Assays for the ability of a microorganism to assimilate peptide as a sole source of carbon and energy. D-tartaric acid assimilation assay Assays for the ability of a microorganism to assimilate D-tartaric acid (D-tartrate) as a sole source of carbon and energy. Carrine Blank D-tartaric acid D-tartrate D-tartaric acid assimilation talose assimilation assay talose assimilation talose Assays for the ability of a microorganism to assimilate talose as a sole source of carbon and energy. Carrine Blank D-talose assimilation assay Assays for the ability of a microorganism to assimilate D-talose as a sole source of carbon and energy. Carrine Blank D-talose assimilation D-talose D-tlose D-sorbose assimilation assay D-sorbose Assays for the ability of a microorganism to assimilate D-sorbose as a sole source of carbon and energy. D-sorbose assimilation Carrine Blank L-ribose assimilation assay L-ribose Carrine Blank L-ribose assimilation Assays for the ability of a microorganism to assimilate L-ribose as a sole source of carbon and energy. alpha-L-rhamnopyranose assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate alpha-L-rhamnopyranose as a sole source of carbon and energy. alpha-L-rhamnopyranose a-L-rhamnose alpha-L-rhamnose alpha-L-rhamnopyranose assimilation methylamines assimilation assay Assays for the ability of a microorganism to assimilate methylamines as a sole source of carbon and energy. Carrine Blank methylamines methylamines assimilation amine assimilation assay amine assimilation Assays for the ability of a microorganism to assimilate amine as a sole source of carbon and energy. Carrine Blank amine organic amino compound assimilation assay organic amino compound Carrine Blank Assays for the ability of a microorganism to assimilate organic amino compound as a sole source of carbon and energy. organic amino compound assimilation D-rhamnose assimilation assay D-rhamnose assimilation Assays for the ability of a microorganism to assimilate D-rhamnose as a sole source of carbon and energy. D-rhamnose Carrine Blank information biomacromolecule assimilation assay information biomacromolecule assimilation Assays for the ability of a microorganism to assimilate information biomacromolecule as a sole source of carbon and energy. information biomacromolecule Carrine Blank biomacromolecule assimilation assay biomacromolecule Carrine Blank Assays for the ability of a microorganism to assimilate biomacromolecule as a sole source of carbon and energy. biomacromolecule assimilation exopolysaccharide assimilation assay Assays for the ability of a microorganism to assimilate exopolysaccharide as a sole source of carbon and energy. exopolysaccharide exopolysaccharide assimilation Carrine Blank ketone assimilation assay ketone assimilation Assays for the ability of a microorganism to assimilate ketone as a sole source of carbon and energy. Carrine Blank ketone methylamines fermentation assay Carrine Blank methylamines fermentation Assays for the ability of a microorganism to ferment methylamines. methylamines acidification methylamines alpha-L-rhamnopyranose fermentation assay alpha-L-rhamnopyranose Assays for the ability of a microorganism to ferment alpha-L-rhamnopyranose. alpha-L-rhamnopyranose acidification Carrine Blank alpha-L-rhamnopyranose fermentation a-L-rhamnose alpha-L-rhamnose D-rhamnose fermentation assay D-rhamnose acidification Assays for the ability of a microorganism to ferment D-rhamnose. Carrine Blank D-rhamnose fermentation D-rhamnose N-acetyl-D-mannosamine fermentation assay N-acetyl-D-manosamine Assays for the ability of a microorganism to ferment N-acetyl-D-mannosamine. N-acetyl-D-mannosamine N-acetyl-D-mannosamine fermentation Carrine Blank N-acetyl-D-mannosamine acidification mixture fermentation assay mixture acidification mixture fermentation mixture Assays for the ability of a microorganism to ferment mixture. Carrine Blank mixture metabolic assay Carrine Blank Assays for the ability of a microorganize to metabolize a mixture. D-threonine fermentation assay D-threonine D-threonine fermentation Carrine Blank Assays for the ability of a microorganism to ferment D-threonine. D-threonine acidification L-tartartic acid fermentation assay L-tartartic acid acidification Carrine Blank L-tartrate L-tartartic acid L-tartartic acid fermentation Assays for the ability of a microorganism to ferment L-tartartic acid (L-tartrate). D-tartaric acid fermentation assay D-tartaric acid D-tartaric acid fermentation D-tartaric acid acidification Assays for the ability of a microorganism to ferment D-tartaric acid (D-tartrate). D-tartrate Carrine Blank talose fermentation assay Assays for the ability of a microorganism to ferment talose. Carrine Blank talose acidification talose fermentation talose D-talose fermentation assay Carrine Blank D-talose fermentation Assays for the ability of a microorganism to ferment D-talose. D-talose acidification D-talose D-tlose D-sorbose fermentation assay Assays for the ability of a microorganism to ferment D-sorbose. D-sorbose D-sorbose fermentation Carrine Blank D-sorbose acidification L-ribose fermentation assay Carrine Blank L-ribose fermentation L-ribose acidification L-ribose Assays for the ability of a microorganism to ferment L-ribose. L-tartartic acid assimilation assay Assays for the ability of a microorganism to assimilate L-tartartic acid (L-tartrate) as a sole source of carbon and energy. Carrine Blank L-tartrate L-tartartic acid assimilation L-tartartic acid D-threonine assimilation assay D-threonine assimilation Carrine Blank D-threonine Assays for the ability of a microorganism to assimilate D-threonine as a sole source of carbon and energy. L-xylose assimilation assay L-xylose Lxylose L-xylose assimilation Assays for the ability of a microorganism to assimilate L-xylose as a sole source of carbon and energy. Carrine Blank chemical entity metabolic assay Carrine Blank Assays for the ability of a microorganism to metabolize (oxidize, reduce, or ferment) a chemical entity as a sole source of carbon and/or energy. mixture assimilation assay Assays for the ability of a microorganism to assimilate mixture as a sole source of carbon and energy. mixture mixture assimilation Carrine Blank undefined organic chemical mixture assimilation assay Assays for the ability of a microorganism to assimilate undefined organic chemical mixture as a sole source of carbon and energy. undefined organic chemical mixture Carrine Blank undefined organic chemical mixture assimilation yeast extract assimilation assay yeast extract Assays for the ability of a microorganism to assimilate yeast extract as a sole source of carbon and energy. Carrine Blank yeast extract assimilation tryptone assimilation assay tryptone assimilation bacto-tryptone tryptone Carrine Blank Assays for the ability of a microorganism to assimilate tryptone as a sole source of carbon and energy. Trypticase peptone assimilation assay Carrine Blank bio-trypcase tryptic peptone Trypticase peptone assimilation Assays for the ability of a microorganism to assimilate Trypticase peptone as a sole source of carbon and energy. trypticase bio-trypticase Trypticase peptone soy peptone assimilation assay soytone Assays for the ability of a microorganism to assimilate soy peptone as a sole source of carbon and energy. Carrine Blank bacto soytone soy peptone soy peptone assimilation soya peptone proteose peptone assimilation assay proteose peptone proteose peptone assimilation bacto proteose peptone Carrine Blank Assays for the ability of a microorganism to assimilate proteose peptone as a sole source of carbon and energy. protein assimilation assay proteins Carrine Blank Assays for the ability of a microorganism to assimilate protein as a sole source of carbon and energy. protein assimilation proteinaceous substrates protein peptone assimilation assay peptones peptone Assays for the ability of a microorganism to assimilate peptone as a sole source of carbon and energy. bacto peptone bacterial peptone peptone assimilation Carrine Blank diol fermentation assay diol acidification diol fermentation diol Carrine Blank Assays for the ability of a microorganism to ferment diol. chopped meat fermentation assay chopped meat chopped meat acidification Carrine Blank Assays for the ability of a microorganism to ferment chopped meat. chopped meat fermentation meat products peptone fermentation assay Assays for the ability of a microorganism to ferment peptone substrates. Carrine Blank peptone peptones peptone acidification peptone fermentation bacto peptone bacterial peptone protein fermentation assay Assays for the ability of a microorganism to ferment protein. proteinaceous substrates protein acidification proteins protein fermentation Carrine Blank protein proteose peptone fermentation assay bacto proteose peptone proteose peptone Assays for the ability of a microorganism to ferment proteose peptone. proteose peptone acidification Carrine Blank proteose peptone fermentation soy peptone fermentation assay soytone soya peptone soy peptone acidification Assays for the ability of a microorganism to ferment soy peptone. Carrine Blank soy peptone fermentation bacto soytone soy peptone Trypticase peptone fermentation assay Trypticase peptone bio-trypcase bio-trypticase Trypticase peptone acidification tryptic peptone trypticase Assays for the ability of a microorganism to ferment Trypticase peptone. Trypticase peptone fermentation Carrine Blank tryptone fermentation assay tryptone acidification tryptone fermentation Assays for the ability of a microorganism to ferment tryptone. tryptone Carrine Blank bacto-tryptone yeast extract fermentation assay yeast extract fermentation yeast extract Assays for the ability of a microorganism to ferment yeast extract. yeast extract acidification Carrine Blank undefined organic chemical mixture fermentation assay Carrine Blank undefined organic chemical mixture Assays for the ability of a microorganism to ferment undefined organic chemical mixture. undefined organic chemical mixture fermentation undefined organic chemical mixture acidification chopped meat assimilation assay Assays for the ability of a microorganism to assimilate chopped meat as a sole source of carbon and energy. chopped meat assimilation chopped meat Carrine Blank meat products diol assimilation assay Assays for the ability of a microorganism to assimilate diol as a sole source of carbon and energy. Carrine Blank diol assimilation diol casitone assimilation assay casein peptone Assays for the ability of a microorganism to assimilate casitone as a sole source of carbon and energy. Carrine Blank casitone casitone assimilation casein hydrolysate assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate casein hydrolysate as a sole source of carbon and energy. casein hydrolysate casein hydrolysate assimilation casein assimilation assay milk peptonization Assays for the ability of a microorganism to assimilate casein as a sole source of carbon and energy. caseinolytic Carrine Blank casein casein assimilation benzyl alcohol assimilation assay benzyl alcohol Assays for the ability of a microorganism to assimilate benzyl alcohol as a sole source of carbon and energy. benzyl alcohol assimilation Carrine Blank benzene assimilation assay benzene Assays for the ability of a microorganism to assimilate benzene as a sole source of carbon and energy. benzene assimilation Carrine Blank nicotinate fermentation assay nicotinate fermentation Assays for the ability of a microorganism to ferment nicotinate (nicotinic acid). nicotinic acid Carrine Blank nicotinate nicotinate acidification orotic acid fermentation assay Carrine Blank orotate orotic acid fermentation orotic acid orotic acid acidification Assays for the ability of a microorganism to ferment orotic acid (orotate). salicylic acid fermentation assay salicylic acid acidification Assays for the ability of a microorganism to ferment salicylic acid (salicylate). Carrine Blank salicylic acid salicylic acid fermentation salicylate sorbic acid fermentation assay sorbic acid acidification Carrine Blank sorbic acid fermentation Assays for the ability of a microorganism to ferment sorbic acid (sorbate). sorbic acid sorbate alpha-mannosidase assay with BrNaphthol 6-bromo-2-naphthyl-a-mannosidase Carrine Blank 6-Br-2-naphthyl-alpha-mannosidase 6-Br-2-naphthyl-a-mannosidase 6-bromo-2-naphthyl-alpha-mannosidase An assay for the activity of alpha-mannosidase in a microorganism. An assay for alpha-mannosidase activity using 6-bromo-2-naphthyl-alpha-L-mannoside. Alpha-mannosidase will cleave the substrate, producing 6-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (a bromo subsituted azo dye) that is purple in color. casein fermentation assay Carrine Blank Assays for the ability of a microorganism to ferment casein. casein acidification casein milk peptonization caseinolytic casein fermentation casein hydrolysate fermentation assay casein hydrolysate casein hydrolysate fermentation Assays for the ability of a microorganism to ferment casein hydrolysate. casein hydrolysate acidification Carrine Blank casitone fermentation assay casitone acidification casitone fermentation Carrine Blank casein peptone casitone Assays for the ability of a microorganism to ferment casitone. Carrine Blank beta-glucuronidase with resorufin Carrine Blank bGUR An assay for the activity of beta-glucuronidase in a microorganism. Uses the substrate resorufin-beta-D-glucuronide. Beta-glucuronidase will cleave the substrate, producing resorufin, which is pink. A positive result is fluorescent pink/red-orange; a negative result is orange. L-proline arylamidase assay using methoxy-NA Carrine Blank L-proline 4-methoxy-2-naphthylamide Uses the substrate L-proline 4-methoxy-2-naphthylamide at pH 7.5. Proline arylamidase activity (which could be from proline aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. ProA L-arginine dihydrolase assay ADH The purpose of this assay is to determine if a microbial isolate is capable of metabolizing arginine under anaerobic conditions. When arginine is metabolized, the pH of the medium increases due to the accumulation of ammonia and organic amines (e.g. putrescine). This turns color of the pH indicator (e.g. bromcresol purple, cresol red) to red/orange. A positive test yields a red/orange color; a negative test is yellow. Arginine dihydrolase (arginine deiminase) catalyzes the reaction: L-arginine + H2O <=> L-citrulline + NH3a Carrine Blank chymotrypsin assay using Glu-Phe-pNA N-glutaryl-phenylalanine-2-naphthylamide Chymotrypsin assay that uses the substrate N-glutaryl-phenylalanine 2-naphthylamide. Trypsin activity will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color. Carrine Blank gamma glutamyl transferase assay gamma glutamyl transferase An amino acid arylamidase assay that measures the activity of gamma glutamyl transferase. gamma-glutamic acid arylamidase g-glutamyltranferase gamma-glutamate aminopeptidase gamma-gluatmic acid aminopeptidase Carrine Blank g-glutamyl transferase GGT gamma-glutamate arylamidase gamma-glutamyltranferase 5-gamma-glutamylaminopeptidase activity beta-D-fucosidase assay Carrine Blank beta-D-fucopyranosidase An assay for the activity of beta-D-fucosidase in a microorganism using various chromogenic substrates. beta-D-fucosidase BdFUC L-fucosidase activity Carrine Blank L-proline arylamidase assay Carrine Blank An alpha-amino acid arylamidase assay. Proline arylamidase activity (which could be from proline aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing a colored product. proline arylamidase proline aminopeptidase ProA PRO beta-L-fucosidase activity Carrine Blank L-arginine arylamidase assay using methoxy-NA Carrine Blank ArgA An L-arginine arylamidase assay that uses the substrate L-arginine-4-methoxy-2-naphthylamide (L-arginine-4-methoxy-beta-naphthylamide) at pH 7.5. Arginine arylamidase activity (which could be from arginine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. L-arginine arylamidase assay ARG Carrine Blank arginine aminopeptidase An alpha-amino acid arylamidase assay that assays for L-arginine arylamidase activity. arginine arylamidase ArgA D-fucosidase activity Carrine Blank L-arabinofuranosidase activity Carrine Blank beta-mannosidase assay beta-mannosidase b-mannosidase BMAN Carrine Blank A carbohydrate hydrolysis assay for the activity of beta-mannosidase in a microorganism. L-arabinosidase activity Carrine Blank N-acetyl-beta-glucosaminidase assay with BrNaphthol bNAG Carrine Blank An assay for the activity of N-acetyl-beta-glucosaminidase in a microorganism. Uses the substrate 6-bromo-2-naphthyl-N-acetyl-beta-D-glucosaminide. N-acetyl-beta-glucosaminidase activity (catalyzing the hydrolysis of terminal beta-N-acetylglucosamine residues from oligosaccharides) will cleave the substrate, producing 6-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is brown in color. beta-mannosidase assay using pNP p-nitrophenyl-b-D-mannopyranoside Carrine Blank bMAN 4-nitrophenyl-beta-D-mannoside 4-nitrophenyl-beta-D-mannopyranoside 4-nitrophenyl-b-D-mannoside A carbohydrate hydrolysis assay for the activity of beta-mannosidase in a microorganism using the substrate 4-nitrophenyl-beta-D-mannopyranoside. beta-Mannosidase activity (catalyzing the hydrolysis of terminal non-reducing beta-D-mannose residues in beta-D-mannosides) will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. 4-nitrophenyl-b-D-mannopyranoside p-nitrophenyl-beta-D-mannopyranoside L-arabinofuranosidase assay An L-arabinosidase assay for the activity of L-arabinofuranosidase in a microorganism using various chromogenic substrates which carries out the hydrolysis of terminal L-arabinofuranoside residues in alpha-L-arabinofuranosides. L-arabinofuranosidase AARA Carrine Blank L-arabinosidase assay L-arabinosidase A carbohydrate hydrolysis assay for the activity of L-arabinosidase in a microorganism using various chromogenic substrates which carries out the hydrolysis of terminal L-arabinoside residues in L-arabinosides. Carrine Blank gamma glutamyl transferase assay using nitroanilide GGT L-glutamic acid gamma-(4-nitroanilide) gamma-L-glutamate-p-nitroanilide γ-Glutamyl ρ-nitroanilide An amino acid arylamidase assay that measures the activity of gamma glutamyl transferase using L-glutamic acid gamma-(4-nitroanilide). Gamma-glutamyl transferase activity cleaves the substrate, releasing p-nitroaniline which is bright yellow in color. A positive test is yellow; a negative test is colorless. Carrine Blank alpha-L-fucosidase assay AlFUC alpha-L-fucosidase alpha-L-fucopyranosidase Carrine Blank gamma glutamyl transferase assay using carboxy-nitroanilide An amino acid arylamidase assay that measures the activity of gamma glutamyl transferase using L-glutamic acid gamma-(3-carboxy-4-nitroanilide). Gamma-glutamyl transferase activity cleaves the substrate, releasing 3-carboxy-4-nitroaniline which is bright yellow in color. A positive test is yellow; a negative test is colorless. Carrine Blank bovine serum albumin assimilation assay bovine serum albumin assimilation Carrine Blank Assays for the ability of a microorganism to assimilate bovine serum albumin as a sole source of carbon and energy. bovine serum albumin diphenylamine assimilation assay Carrine Blank diphenylamine assimilation Assays for the ability of a microorganism to assimilate diphenylamine as a sole source of carbon and energy. diphenylamine fibrin assimilation assay Carrine Blank fibrin Assays for the ability of a microorganism to assimilate fibrin as a sole source of carbon and energy. fibrin assimilation fucoidan assimilation assay fucoidan Assays for the ability of a microorganism to assimilate fucoidan as a sole source of carbon and energy. Carrine Blank fucoidan assimilation galactan assimilation assay Carrine Blank Assays for the ability of a microorganism to assimilate galactan as a sole source of carbon and energy. galactan assimilation galactan galactomannan assimilation assay Assays for the ability of a microorganism to assimilate galactomannan as a sole source of carbon and energy. galactomannan assimilation galactomannan Carrine Blank methyl-3,4-dimethoxycinnamate assimilation assay methyl-3,4-dimethoxycinnamate Carrine Blank methyl-3,4-dimethoxycinnamate assimilation methyl-3,4-dimethoxycinnamic acid Assays for the ability of a microorganism to assimilate methyl-3,4-dimethoxycinnamate (methyl-3,4-dimethoxycinnamic acid) as a sole source of carbon and energy. naphthalene assimilation assay naphthalene assimilation naphthalene Assays for the ability of a microorganism to assimilate naphthalene as a sole source of carbon and energy. Carrine Blank nicotinate assimilation assay Carrine Blank nicotinate assimilation nicotinate nicotinic acid Assays for the ability of a microorganism to assimilate nicotinate (nicotinic acid) as a sole source of carbon and energy. orotic acid assimilation assay orotic acid orotate orotic acid assimilation Assays for the ability of a microorganism to assimilate orotic acid (orotate) as a sole source of carbon and energy. Carrine Blank salicylic acid assimilation assay salicylate Carrine Blank Assays for the ability of a microorganism to assimilate salicylic acid (salicylate) as a sole source of carbon and energy. salicylic acid assimilation salicylic acid naphthalene fermentation assay naphthalene acidification naphthalene naphthalene fermentation Assays for the ability of a microorganism to ferment naphthalene. Carrine Blank methyl-3,4-dimethoxycinnamate fermentation assay methyl-3,4-dimethoxycinnamate methyl-3,4-dimethoxycinnamate fermentation Carrine Blank methyl-3,4-dimethoxycinnamic acid methyl-3,4-dimethoxycinnamate acidification Assays for the ability of a microorganism to ferment methyl-3,4-dimethoxycinnamate (methyl-3,4-dimethoxycinnamic acid). galactomannan fermentation assay Assays for the ability of a microorganism to ferment galactomannan. galactomannan acidification galactomannan Carrine Blank galactomannan fermentation galactan fermentation assay galactan fermentation Carrine Blank Assays for the ability of a microorganism to ferment galactan. galactan acidification galactan fucoidan fermentation assay Carrine Blank fucoidan acidification fucoidan fucoidan fermentation Assays for the ability of a microorganism to ferment fucoidan. fibrin fermentation assay fibrin acidification fibrin Carrine Blank Assays for the ability of a microorganism to ferment fibrin. fibrin fermentation diphenylamine fermentation assay diphenylamine fermentation diphenylamine Carrine Blank diphenylamine acidification Assays for the ability of a microorganism to ferment diphenylamine. bovine serum albumin fermentation assay bovine serum albumin bovine serum albumin acidification bovine serum albumin fermentation Carrine Blank Assays for the ability of a microorganism to ferment bovine serum albumin. benzyl alcohol fermentation assay benzyl alcohol benzyl alcohol fermentation benzyl alcohol acidification Assays for the ability of a microorganism to ferment benzyl alcohol. Carrine Blank benzene fermentation assay benzene acidification Carrine Blank Assays for the ability of a microorganism to ferment benzene. benzene benzene fermentation sorbic acid assimilation assay sorbate Assays for the ability of a microorganism to assimilate sorbic acid (sorbate) as a sole source of carbon and energy. sorbic acid assimilation Carrine Blank sorbic acid soya extract assimilation assay soya extract assimilation soya extract Assays for the ability of a microorganism to assimilate soya extract as a sole source of carbon and energy. Carrine Blank trimethylamine N-oxide assimilation assay trimethylamine N-oxide assimilation Carrine Blank Assays for the ability of a microorganism to assimilate trimethylamine N-oxide as a sole source of carbon and energy. trimethylamine N-oxide benzenoid aromatic compound assimilation assay Assays for the ability of a microorganism to assimilate benzenoid aromatic compound as a sole source of carbon and energy. benzenoid aromatic compound assimilation benzenoid aromatic compound Carrine Blank organic aromatic compound assimilation assay Assays for the ability of a microorganism to assimilate organic aromatic compound as a sole source of carbon and energy. organic aromatic compound Carrine Blank organic aromatic compound assimilation keratin assimilation assay keratin Assays for the ability of a microorganism to assimilate keratin as a sole source of carbon and energy. keratin assimilation Carrine Blank Carrine Blank beta-glucosidase with resorufin An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate resorufin-beta-D-glucopyranoside. Beta-glucosidase will cleave the substrate, producing resorufin, which is pink. A positive result is fluorescent pink/red-orange; a negative result is orange. bGLU Carrine Blank soya extract fermentation assay soya extract acidification Carrine Blank soya extract Assays for the ability of a microorganism to ferment soya extract. soya extract fermentation trimethylamine N-oxide fermentation assay trimethylamine N-oxide acidification trimethylamine N-oxide fermentation Carrine Blank Assays for the ability of a microorganism to ferment trimethylamine N-oxide. trimethylamine N-oxide benzenoid aromatic compound fermentation assay Carrine Blank benzenoid aromatic compound acidification Assays for the ability of a microorganism to ferment benzenoid aromatic compound. benzenoid aromatic compound benzenoid aromatic compound fermentation organic aromatic compound fermentation assay Assays for the ability of a microorganism to ferment organic aromatic compound. organic aromatic compound organic aromatic compound acidification organic aromatic compound fermentation Carrine Blank Carrine Blank alkaline phosphatase assay using pNP-CHA Alkaline phosphatase assay that uses the substrate 4-nitrophenyl-beta-D-galactopyranoside-2-CHA (para-nitrophenyl-beta-D-galactopyranoside-2-CHA). Under alkaline conditions, alkaline phosphatase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. Carrine Blank 4-nitrophenyl-bD-galactopyranoside-2-CHA PAL keratin fermentation assay keratin keratin acidification Assays for the ability of a microorganism to ferment keratin. keratin fermentation Carrine Blank isovanillin-8 pubchem term is: 3-Hydroxy-4-methoxybenzaldehyde 6-bromo-2-naphthyl alpha-D-mannoside-9 4-nitrophenyl beta-D-fucoside-8 4-nitrophenyl-beta-D-galactopyranoside-2-CHA-8 cyclohexyl ammonium salt, e.g. http://www.melford.co.uk/index.php?sid=396401522&t=sub_pages&cat=39 4-nitroaniline-8 resorufin-beta-D-glucuronide-8 resorufin-beta-D-glucopyranoside-8 resorufin-beta-D-galactopyranoside-8 resorufin-8 N-(gamma-L-glutamyl)-4-methoxy-2-naphthylamide-8 L-proline 4-methoxy-2-naphthylamide-8 L-arginine 4-methoxy-2-naphthylamide-8 pubchem term is: Arg-MNA 6-bromo-2-naphthyl-N-acetyl-beta-D-glucosaminide-8 4-nitrophenyl-beta-D-mannopyranoside-8 3-carboxy-4-nitroaniline-8 pubchem term is 5-Amino-2-nitrobenzoic acid temp2 temp3 temp4 temp5 temp6 temp7 temp8 temp9 L-glutamic acid gamma-(3-carboxy-4-nitroanilide)-8 L-glutamic acid gamma-(4-nitroanilide)-8 L-proline 4-nitroanilide-8 temp13 temp14 temp15 temp16 temp17 temp18 temp19 temp20 n cells Unit of cell width defined as an integer number of cells (i.e. 2 cells' width or 3 cells' width). degrees Carrine Blank Temperature, in degrees Celsius. pH Wikipedia: pH In chemistry, pH (/piːˈeɪtʃ/) is a numeric scale used to specify the acidity or alkalinity of an aqueous solution. It is the negative of the logarithm to base 10 of the activity of the hydrogen ion. Solutions with a pH less than 7 are acidic and solutions with a pH greater than 7 are alkaline or basic. Pure water is neutral, being neither an acid nor a base. Contrary to popular belief, the pH value can be less than 0 or greater than 14 for very strong acids and bases respectively.[1] The log of the hydrogen ion concentration. Carrine Blank atmospheres Unit of atmospheric pressure at the surface of the Earth. um Carrine Blank Micrometers (or microns), a measurement of length, 10^-6 of a meter. Carrine Blank percent A measurement of concentration of solution in weight per 100 g (w/w), or in weight per 100 mL (w/v). Carrine Blank