is about
has_specified_input
has_specified_output
achieves_planned_objective
sequence-specific DNA binding
data item
genetically modified material
protein and DNA interaction identification objective
yeast one-hybrid assay
bacterial one-hybrid assay
An assay that identifies the sequence-specific target site of a DNA-binding domain. In this system, a given transcription factor (TF) is expressed as a fusion to a subunit of RNA polymerase. In parallel, a library of randomized oligonucleotides representing potential TF target sequences, is cloned into a separate vector containing the selectable genes HIS3 and URA3. If the DNA-binding domain (bait) binds a potential DNA target site (prey) in vivo, it will recruit RNA polymerase to the promoter and activate transcription of the reporter genes in that clone. The two reporter genes, HIS3 and URA3, allow for positive and negative selections, respectively. At the end of the process, positive clones are sequenced and examined with motif-finding tools in order to resolve the favoured DNA target sequence
bacterial one-hybrid assay
Marcus Chibucos
Philippe Rocca-Serra
A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. PMID:18332042
url:http://en.wikipedia.org/wiki/Bacterial_one-hybrid_system
B1H
ready for release